Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,999,772 T→C H4R (CAC→CGC)  b1922 ← sigma factor 28

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,999,7720TC100.0% 635.0 / NA 198H4R (CAC→CGC) b1922sigma factor 28
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (91/107);  total (91/107)

Only 100 of 204 total aligned reads displayed.

ATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT  >  NC_000913/1999684‑1999861
                                                                                        |                                                                                         
 tCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTa                                                                                      <  2:4103450/93‑1 (MQ=255)
 acGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTa                                                                                      <  2:5018774/92‑1 (MQ=255)
  cGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTAt                                                                                     <  1:2580035/93‑1 (MQ=255)
  cGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTAt                                                                                     <  2:3401091/93‑1 (MQ=255)
  cGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTAt                                                                                     >  2:586569/1‑93 (MQ=255)
   gTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATc                                                                                    >  1:4912592/1‑93 (MQ=255)
   gTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATc                                                                                    >  2:3963241/1‑93 (MQ=255)
   gTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATc                                                                                    >  2:1927384/1‑93 (MQ=255)
    tCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATcc                                                                                   >  2:2628336/1‑93 (MQ=255)
     cAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCa                                                                                  <  1:5982545/93‑1 (MQ=255)
         ttCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTac                                                                              <  1:1435042/93‑1 (MQ=255)
           ccACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTacac                                                                            <  2:5410659/93‑1 (MQ=255)
            cACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACAcc                                                                           >  2:204124/1‑93 (MQ=255)
            cACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACAcc                                                                           >  1:1729570/1‑93 (MQ=255)
            cACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACAcc                                                                           <  1:4769245/93‑1 (MQ=255)
              cGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCtt                                                                         >  2:3220456/1‑93 (MQ=255)
                cTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCa                                                                       <  2:1847527/93‑1 (MQ=255)
                 tCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGCGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAg                                                                      <  1:2457558/93‑1 (MQ=255)
                   gcgGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCg                                                                    <  2:4820410/93‑1 (MQ=255)
                   gcgGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCg                                                                    >  1:2504969/1‑93 (MQ=255)
                    cgGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCgg                                                                   >  1:3949303/1‑93 (MQ=255)
                    cgGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCgg                                                                   <  1:4125291/93‑1 (MQ=255)
                      ggCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGta                                                                 >  2:4890242/1‑93 (MQ=255)
                      ggCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGta                                                                 >  1:6224293/1‑93 (MQ=255)
                         aGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATag                                                              >  1:2035497/1‑93 (MQ=255)
                          gTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATaga                                                             >  2:1887373/1‑93 (MQ=255)
                           tCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATagag                                                            <  1:5906629/93‑1 (MQ=255)
                            cGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACACAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGt                                                           <  2:1502138/93‑1 (MQ=255)
                              aaCCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGa                                                         >  1:904614/1‑93 (MQ=255)
                               aCCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGaa                                                        >  2:4573486/1‑93 (MQ=255)
                                 cTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAAtt                                                      >  1:2230768/1‑93 (MQ=255)
                                 cTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAAtt                                                      >  2:1758887/1‑93 (MQ=255)
                                  tGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTc                                                     <  2:3528304/93‑1 (MQ=255)
                                   gCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCa                                                    >  2:711746/1‑93 (MQ=255)
                                    gaGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCAc                                                   <  2:5222860/92‑1 (MQ=255)
                                      ggCGCAATGCTTCGTGACGCGCCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGa                                                 <  1:1923717/93‑1 (MQ=255)
                                      ggCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGa                                                 <  2:5555161/93‑1 (MQ=255)
                                      ggCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGa                                                 <  1:5370643/93‑1 (MQ=255)
                                         gcAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATaa                                              >  1:3543659/1‑93 (MQ=255)
                                         gcAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATaa                                              >  1:998534/1‑93 (MQ=255)
                                          cAATGCTTCGTGTGGCACCAGCGGTACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATaaa                                             <  2:5113479/93‑1 (MQ=255)
                                          cAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATaaa                                             <  1:3879208/93‑1 (MQ=255)
                                          cAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATaaa                                             >  2:5486771/1‑93 (MQ=255)
                                           aaTGCTTCTTGACGCACCACCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAAc                                            >  2:6143887/1‑93 (MQ=255)
                                           aaTGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAAc                                            >  2:5055729/1‑93 (MQ=255)
                                           aaTGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAAc                                            >  1:1770934/1‑93 (MQ=255)
                                             tGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAg                                          <  1:2813221/93‑1 (MQ=255)
                                             tGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAg                                          <  1:6273389/93‑1 (MQ=255)
                                                ttCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGCGAATTCACGATAAACAGccc                                       >  1:4256681/1‑93 (MQ=255)
                                                 tCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCt                                      >  1:1340727/1‑93 (MQ=255)
                                                   gttaCGCACAAGCTATACATAACGCTTCCCCAGCGAGCGTTTCTCCATTACCCCTTCAGCGGTATAGAGTAAATTCACGATAAACAGCCCTGc                                    <  2:2697439/90‑1 (MQ=255)
                                                   gTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGc                                    <  2:2740727/93‑1 (MQ=255)
                                                   gTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGc                                    <  2:1636285/93‑1 (MQ=255)
                                                   gTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGc                                    <  2:4219253/93‑1 (MQ=255)
                                                   gTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGc                                    >  2:3729988/1‑93 (MQ=255)
                                                     gACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGt                                  >  1:5616586/1‑93 (MQ=255)
                                                     gACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGt                                  <  2:2478080/93‑1 (MQ=255)
                                                     gACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGt                                  >  1:446358/1‑93 (MQ=255)
                                                      aCGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGtt                                 <  1:2962410/93‑1 (MQ=255)
                                                        gCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTtat                               <  1:1669462/93‑1 (MQ=255)
                                                         cACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTtata                              >  2:2863466/1‑93 (MQ=255)
                                                         cACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTtata                              <  1:5823097/93‑1 (MQ=255)
                                                           ccAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAACCAGCCCTGCGTTATATg                            <  1:3423314/93‑1 (MQ=255)
                                                           ccAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATg                            <  2:2951254/93‑1 (MQ=255)
                                                            cAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGa                           <  2:1806006/93‑1 (MQ=255)
                                                            cAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGa                           <  2:2192523/93‑1 (MQ=255)
                                                            cAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGa                           >  1:1498993/1‑93 (MQ=255)
                                                             aGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAg                          <  2:1200746/93‑1 (MQ=255)
                                                              gCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGt                         >  1:2679280/1‑93 (MQ=255)
                                                              gCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGt                         <  1:6192349/93‑1 (MQ=255)
                                                                 ggACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTAt                      <  1:4819821/93‑1 (MQ=255)
                                                                 ggACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTAt                      <  1:2163902/93‑1 (MQ=255)
                                                                 ggACATAACGCTGCCACAGCGAGCGTTTATCCATGACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTAt                      <  1:5412354/93‑1 (MQ=255)
                                                                  gACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATc                     <  2:943549/93‑1 (MQ=255)
                                                                  gACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATc                     <  2:2270964/93‑1 (MQ=255)
                                                                   aCATAACGCTGCCACAGCGAGCTGTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCg                    <  1:5451754/93‑1 (MQ=255)
                                                                   aCATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCg                    <  2:2912297/93‑1 (MQ=255)
                                                                   aCATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCg                    >  1:4986448/1‑93 (MQ=255)
                                                                     aTAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCgga                  >  1:2132865/1‑92 (MQ=255)
                                                                      tAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCa                 >  1:168091/1‑93 (MQ=255)
                                                                       aaCGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCAt                >  2:3493115/1‑93 (MQ=255)
                                                                        aCGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATg               >  1:3535630/1‑93 (MQ=255)
                                                                        aCGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATg               >  1:1146849/1‑93 (MQ=255)
                                                                        aCGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATg               <  2:5732723/93‑1 (MQ=255)
                                                                          gCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGAt             <  1:3652590/93‑1 (MQ=255)
                                                                            tGCCACAGCGAGCGTTTATTCATTACACCTTCAGCGGTATAGAGTGAATTCACGATCAACAGCCCTGCGTTATATGAGTTCTCGGCATGAttt           >  1:2727839/1‑92 (MQ=255)
                                                                            tGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTa           <  1:3507951/93‑1 (MQ=255)
                                                                             gCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTAt          <  1:2538854/93‑1 (MQ=255)
                                                                             gCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTAt          >  1:1630835/1‑93 (MQ=255)
                                                                              ccACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATc         <  1:4338943/93‑1 (MQ=255)
                                                                              ccACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATc         <  1:2643580/93‑1 (MQ=255)
                                                                               cacaGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATcc        <  1:1252573/93‑1 (MQ=255)
                                                                               cacaGCGAGCGTTTATCCAGTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATcc        <  2:1549866/93‑1 (MQ=255)
                                                                                acaGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCg       >  1:509715/1‑93 (MQ=255)
                                                                                acaGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCg       >  1:2822523/1‑93 (MQ=255)
                                                                                   gCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGttt    <  2:3949303/93‑1 (MQ=255)
                                                                                   gCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGttt    >  2:3680977/1‑93 (MQ=255)
                                                                                    cGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTc   <  1:4631769/93‑1 (MQ=255)
                                                                                    cGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTc   <  2:1340727/93‑1 (MQ=255)
                                                                                     gAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCt  >  1:1018432/1‑93 (MQ=255)
                                                                                        |                                                                                         
ATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT  >  NC_000913/1999684‑1999861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAG  >  NC_000913/1999681‑1999865
                                                                                           |                                                                                             
CAGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGT                                                                                             <  SRR4302533.5379902/94‑1 (MQ=60)
   ATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTA                                                                                          >  SRR4302533.5272324/1‑94 (MQ=60)
    TCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTAT                                                                                         <  SRR4302533.3563348/94‑1 (MQ=60)
     CGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATC                                                                                        >  SRR4302533.3932947/1‑94 (MQ=60)
     CGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATC                                                                                        <  SRR4302533.2595912/94‑1 (MQ=60)
     CGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATC                                                                                        >  SRR4302533.4942351/1‑94 (MQ=60)
        CAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCAT                                                                                     <  SRR4302533.6018777/94‑1 (MQ=60)
         AAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATT                                                                                    >  SRR4302533.588872/1‑94 (MQ=60)
         AAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATT                                                                                    <  SRR4302533.2663642/94‑1 (MQ=60)
           GTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTAC                                                                                  <  SRR4302533.5025443/94‑1 (MQ=60)
            TTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACA                                                                                 <  SRR4302533.1443704/94‑1 (MQ=60)
            TTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACA                                                                                 <  SRR4302533.2514949/94‑1 (MQ=60)
              CCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACC                                                                               <  SRR4302533.283677/94‑1 (MQ=60)
              CCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACC                                                                               >  SRR4302533.1740056/1‑94 (MQ=60)
               CACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCT                                                                              <  SRR4302533.4798152/94‑1 (MQ=60)
                   CTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCGCCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAG                                                                          <  SRR4302533.4183487/94‑1 (MQ=60)
                    TCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGCGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGC                                                                         <  SRR4302533.2472723/94‑1 (MQ=60)
                     CGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCG                                                                        >  SRR4302533.2520412/1‑94 (MQ=60)
                      GCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGG                                                                       >  SRR4302533.3973383/1‑94 (MQ=60)
                      GCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGG                                                                       >  SRR4302533.3973384/1‑94 (MQ=60)
                       CGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGT                                                                      <  SRR4302533.4150334/94‑1 (MQ=60)
                        GGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTA                                                                     >  SRR4302533.6261591/1‑94 (MQ=60)
                         GGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTAT                                                                    >  SRR4302533.1429682/1‑94 (MQ=60)
                           CAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAG                                                                  >  SRR4302533.4595313/1‑94 (MQ=60)
                           CAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAG                                                                  >  SRR4302533.2048050/1‑94 (MQ=60)
                             GTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAG                                                                <  SRR4302533.2397530/94‑1 (MQ=60)
                              TCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGT                                                               <  SRR4302533.5942297/94‑1 (MQ=60)
                                GAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGA                                                             >  SRR4302533.909917/1‑94 (MQ=60)
                                 AACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAA                                                            >  SRR4302533.670557/1‑94 (MQ=60)
                                  ACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAAT                                                           <  SRR4302533.4776742/94‑1 (MQ=60)
                                  ACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAAT                                                           >  SRR4302533.301085/1‑94 (MQ=60)
                                   CCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATT                                                          >  SRR4302533.2555251/1‑94 (MQ=60)
                                   CCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATT                                                          >  SRR4302533.2244532/1‑94 (MQ=60)
                                     TGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCA                                                        >  SRR4302533.2929995/1‑94 (MQ=60)
                                       CAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACG                                                      >  SRR4302533.3519101/1‑94 (MQ=60)
                                       CAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACG                                                      >  SRR4302533.6228687/1‑94 (MQ=60)
                                        AGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGCTTATCCATTACACCTTCAGCGGCATAGAGTGAATTCACGA                                                     >  SRR4302533.561960/1‑94 (MQ=60)
                                         GGCGCAATGCTTCGTGACGCGCCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGAT                                                    <  SRR4302533.1935454/94‑1 (MQ=60)
                                         GGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGAT                                                    <  SRR4302533.5402912/94‑1 (MQ=60)
                                           CGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAA                                                  >  SRR4302533.3565498/1‑94 (MQ=60)
                                           CGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAA                                                  >  SRR4302533.1004363/1‑94 (MQ=60)
                                           CGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAA                                                  <  SRR4302533.4913237/94‑1 (MQ=60)
                                           CGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAA                                                  >  SRR4302533.5403925/1‑94 (MQ=60)
                                           CGCAATGCGTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAA                                                  <  SRR4302533.3608967/94‑1 (MQ=60)
                                            GCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAA                                                 >  SRR4302533.1234589/1‑94 (MQ=60)
                                             CAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAAC                                                >  SRR4302533.1781715/1‑94 (MQ=60)
                                             CAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAAC                                                >  SRR4302533.1039543/1‑94 (MQ=60)
                                             CAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAAC                                                >  SRR4302533.1769451/1‑94 (MQ=60)
                                             CAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAAC                                                <  SRR4302533.2771033/94‑1 (MQ=60)
                                             CAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAAC                                                <  SRR4302533.147794/94‑1 (MQ=60)
                                             CAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAAC                                                <  SRR4302533.3902879/94‑1 (MQ=60)
                                              AATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACA                                               <  SRR4302533.2156960/94‑1 (MQ=60)
                                              AATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACA                                               >  SRR4302533.2109468/1‑94 (MQ=60)
                                                TGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTCCACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGC                                             <  SRR4302533.3545099/94‑1 (MQ=60)
                                                TGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGC                                             <  SRR4302533.2830536/94‑1 (MQ=60)
                                                TGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGC                                             <  SRR4302533.6310859/94‑1 (MQ=60)
                                                  CTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCC                                           <  SRR4302533.1547574/94‑1 (MQ=60)
                                                  CTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGCGAATTCACGATAAACAGCCC                                           >  SRR4302533.4282345/1‑94 (MQ=60)
                                                   TTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCT                                          <  SRR4302533.6296104/94‑1 (MQ=60)
                                                   TTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCT                                          >  SRR4302533.5532787/1‑94 (MQ=60)
                                                   TTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCT                                          >  SRR4302533.1348797/1‑94 (MQ=60)
                                                   TTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCT                                          <  SRR4302533.265681/94‑1 (MQ=60)
                                                      GTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCG                                       <  SRR4302533.3794254/94‑1 (MQ=60)
                                                      GTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCG                                       >  SRR4302533.2730128/1‑94 (MQ=60)
                                                       TGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGT                                      >  SRR4302533.5650540/1‑94 (MQ=60)
                                                       TGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGT                                      >  SRR4302533.449031/1‑94 (MQ=60)
                                                         ACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTA                                    <  SRR4302533.2980520/94‑1 (MQ=60)
                                                           GCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATA                                  <  SRR4302533.1679547/94‑1 (MQ=60)
                                                            CACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATAT                                 <  SRR4302533.5858269/94‑1 (MQ=60)
                                                             ACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATG                                <  SRR4302533.4824778/94‑1 (MQ=60)
                                                              CCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAACCAGCCCTGCGTTATATGA                               <  SRR4302533.3444462/94‑1 (MQ=60)
                                                              CCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGA                               >  SRR4302533.1508026/1‑94 (MQ=60)
                                                                AGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCTGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGT                             <  SRR4302533.4475679/94‑1 (MQ=60)
                                                                AGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGT                             >  SRR4302533.2695703/1‑94 (MQ=60)
                                                                 GCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTT                            <  SRR4302533.6229509/94‑1 (MQ=60)
                                                                 GCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTT                            <  SRR4302533.2531157/94‑1 (MQ=60)
                                                                 GCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTT                            <  SRR4302533.1950807/94‑1 (MQ=60)
                                                                  CGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTA                           <  SRR4302533.544947/94‑1 (MQ=60)
                                                                   GGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTAT                          >  SRR4302533.1313574/1‑94 (MQ=60)
                                                                    GGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATC                         <  SRR4302533.2177215/94‑1 (MQ=60)
                                                                    GGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATC                         <  SRR4302533.4849028/94‑1 (MQ=60)
                                                                    GGACATAACGCTGCCACAGCGAGCGTTTATCCATGACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATC                         <  SRR4302533.5444899/94‑1 (MQ=60)
                                                                     GACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCG                        >  SRR4302533.5016682/1‑94 (MQ=60)
                                                                      ACATAACGCTGCCACAGCGAGCTGTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGG                       <  SRR4302533.5484595/94‑1 (MQ=60)
                                                                      ACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGG                       <  SRR4302533.1655019/94‑1 (MQ=60)
                                                                       CATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGC                      >  SRR4302533.1684422/1‑94 (MQ=60)
                                                                       CATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGA                      >  SRR4302533.2146020/1‑94 (MQ=60)
                                                                        ATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCA                     >  SRR4302533.169152/1‑94 (MQ=60)
                                                                         TAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCAT                    >  SRR4302533.2184946/1‑94 (MQ=60)
                                                                          AACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATG                   >  SRR4302533.6176505/1‑94 (MQ=60)
                                                                          AACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATG                   >  SRR4302533.3557421/1‑94 (MQ=60)
                                                                          AACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATG                   >  SRR4302533.1153694/1‑94 (MQ=60)
                                                                          AACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATG                   <  SRR4302533.6009491/94‑1 (MQ=60)
                                                                             GCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATT                <  SRR4302533.5006079/94‑1 (MQ=60)
                                                                             GCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATT                <  SRR4302533.3675049/94‑1 (MQ=60)
                                                                              CTGCCACAGCGAGCGTTTATTCATTACACCTTCAGCGGTATAGAGTGAATTCACGATCAACAGCCCTGCGTTATATGAGTTCTCGGCATGATTT               >  SRR4302533.2744573/1‑94 (MQ=60)
                                                                              CTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTA               >  SRR4302533.1000303/1‑94 (MQ=60)
                                                                               TGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTAT              <  SRR4302533.3529583/94‑1 (MQ=60)
                                                                               TGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTAT              >  SRR4302533.1640712/1‑94 (MQ=60)
                                                                               TGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTAT              >  SRR4302533.1788354/1‑94 (MQ=60)
                                                                               TGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTAT              <  SRR4302533.4306580/94‑1 (MQ=60)
                                                                                GCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATC             <  SRR4302533.2554486/94‑1 (MQ=60)
                                                                                 CCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCC            <  SRR4302533.6231490/94‑1 (MQ=60)
                                                                                 CCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCC            <  SRR4302533.4365091/94‑1 (MQ=60)
                                                                                 CCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCC            <  SRR4302533.2659824/94‑1 (MQ=60)
                                                                                  CACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCG           >  SRR4302533.512744/1‑94 (MQ=60)
                                                                                  CACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCG           <  SRR4302533.1260062/94‑1 (MQ=60)
                                                                                  CACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCG           >  SRR4302533.2839893/1‑94 (MQ=60)
                                                                                    CAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTT         >  SRR4302533.2848281/1‑94 (MQ=60)
                                                                                       CGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT      >  SRR4302533.5690995/1‑94 (MQ=60)
                                                                                       CGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT      <  SRR4302533.1735909/94‑1 (MQ=60)
                                                                                       CGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT      <  SRR4302533.4659836/94‑1 (MQ=60)
                                                                                       CGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT      >  SRR4302533.1024420/1‑94 (MQ=60)
                                                                                        GAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTG     >  SRR4302533.4488415/1‑94 (MQ=60)
                                                                                         AGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAGCAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGC    <  SRR4302533.941713/94‑1 (MQ=60)
                                                                                         AGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGC    >  SRR4302533.4273191/1‑94 (MQ=60)
                                                                                         AGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGC    <  SRR4302533.4942345/94‑1 (MQ=60)
                                                                                          GCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCA   <  SRR4302533.1707563/94‑1 (MQ=60)
                                                                                          GCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCA   <  SRR4302533.1401750/94‑1 (MQ=60)
                                                                                          GCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCA   <  SRR4302533.5225485/94‑1 (MQ=60)
                                                                                           CGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAG  <  SRR4302533.3419295/94‑1 (MQ=60)
                                                                                           CGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAG  <  SRR4302533.2122315/94‑1 (MQ=60)
                                                                                           |                                                                                             
CAGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAG  >  NC_000913/1999681‑1999865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑