Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,074,403 T→C V25A (GTG→GCG)  yeeS → putative DNA repair protein, RADC family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,074,4030TC99.1% 668.4 / ‑3.9 213V25A (GTG→GCG) yeeSputative DNA repair protein, RADC family
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (103/108);  total (104/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 221 total aligned reads displayed.

GGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGA  >  NC_000913/2074313‑2074492
                                                                                          |                                                                                         
ggTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCgg                                                                                         >  2:4578490/1‑93 (MQ=255)
   aaaTTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCt                                                                                      <  2:3757567/93‑1 (MQ=255)
    aaGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCtt                                                                                     >  2:8072218/1‑93 (MQ=255)
     aGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTc                                                                                    <  2:6143117/93‑1 (MQ=255)
      gTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCa                                                                                   >  1:6648558/1‑93 (MQ=255)
       tAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCAc                                                                                  >  2:811940/1‑93 (MQ=255)
        agagGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCAcc                                                                                 <  2:966993/93‑1 (MQ=255)
         gaggagAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCt                                                                                >  2:3776224/1‑93 (MQ=255)
         gaggagAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCt                                                                                <  2:6206880/93‑1 (MQ=255)
         gaggagAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCt                                                                                >  2:1537593/1‑93 (MQ=255)
            gagagaTGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCa                                                                             <  1:9523302/93‑1 (MQ=255)
            gagagaTGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCa                                                                             >  2:1168269/1‑93 (MQ=255)
              gagaTGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCAcc                                                                           >  1:2504345/1‑93 (MQ=255)
              gagaTGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCAcc                                                                           >  1:7382487/1‑93 (MQ=255)
              gagaTGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCAcc                                                                           <  2:8775706/93‑1 (MQ=255)
               agaTGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCAccc                                                                          >  2:5992309/1‑93 (MQ=255)
                gctGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCGTTCACCTCCACCCg                                                                         >  2:8308908/3‑93 (MQ=255)
                gaTGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCg                                                                         >  1:8718721/1‑93 (MQ=255)
                 aTGGCGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAATCCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGt                                                                        <  1:8466438/93‑1 (MQ=255)
                  tGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGTCTTCACCTCCACCCGTg                                                                       >  1:8970560/1‑93 (MQ=255)
                  cgACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATCAACCCGGCGCGGCCTTCACCTCCCCCCGTg                                                                       <  2:9620594/92‑1 (MQ=255)
                   gACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTgc                                                                      <  2:7753951/93‑1 (MQ=255)
                   gACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTgc                                                                      <  1:6331914/93‑1 (MQ=255)
                   gACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTgc                                                                      <  1:2944660/93‑1 (MQ=255)
                   gACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCATGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTgc                                                                      <  2:7912279/93‑1 (MQ=255)
                       cccGGCGAGCGTAGCCTCATTCAACGGGCCCTTAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTgcagca                                                                  <  1:5233971/93‑1 (MQ=255)
                       cccGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTgcagca                                                                  >  2:9061754/1‑93 (MQ=255)
                       cccGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTgcagca                                                                  <  1:143612/93‑1 (MQ=255)
                       cccGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTgcagca                                                                  <  1:1258813/93‑1 (MQ=255)
                       cccGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTgcagca                                                                  >  2:1901026/1‑93 (MQ=255)
                        ccGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCAc                                                                 >  1:3132131/1‑93 (MQ=255)
                         cGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACg                                                                <  2:7511181/93‑1 (MQ=255)
                          ggCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACgg                                                               <  1:5492881/93‑1 (MQ=255)
                              ggcgcAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAAt                                                           <  1:5777110/92‑1 (MQ=255)
                              aGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAAt                                                           >  1:9727959/1‑93 (MQ=255)
                                 gcAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGc                                                        <  1:8233660/93‑1 (MQ=255)
                                  cAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCt                                                       >  1:282616/1‑93 (MQ=255)
                                  cAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCt                                                       <  1:4460888/93‑1 (MQ=255)
                                  cAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCt                                                       >  2:4285168/1‑93 (MQ=255)
                                   aGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTg                                                      >  2:1791165/1‑93 (MQ=255)
                                    gCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGa                                                     >  1:1976943/1‑93 (MQ=255)
                                    gCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGa                                                     >  1:7831645/1‑93 (MQ=255)
                                     ccTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGAt                                                    <  2:2105695/93‑1 (MQ=255)
                                     ccTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGCGCAGCACGGGAATGGCTGAt                                                    >  2:9312048/1‑93 (MQ=255)
                                      cTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGAtt                                                   <  1:9406555/93‑1 (MQ=255)
                                       tCATTCAACGGGCCTTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTc                                                  >  2:4990484/1‑93 (MQ=255)
                                       tCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTc                                                  >  1:9576122/1‑93 (MQ=255)
                                       tCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTc                                                  <  2:9809150/93‑1 (MQ=255)
                                       tCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTc                                                  >  1:9914859/1‑93 (MQ=255)
                                        cATTCAACGTGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCt                                                 <  2:4683547/93‑1 (MQ=255)
                                        cATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCt                                                 >  1:7565175/1‑93 (MQ=255)
                                         aTTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTg                                                <  1:106528/93‑1 (MQ=255)
                                         aTTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTg                                                <  2:5887139/93‑1 (MQ=255)
                                          ttCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGa                                               >  1:7247644/1‑93 (MQ=255)
                                           tCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTTaa                                              >  1:9237260/1‑93 (MQ=255)
                                           tCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGaa                                              >  2:6635149/1‑93 (MQ=255)
                                           tCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGaa                                              >  1:5002967/1‑93 (MQ=255)
                                            cggcGGGCCCTGTCTACCCTAAACCGCCTTCTTCTTGAACCCGGAGCGGCCTTCACCTACACCCATGCAGAGCGAGAATGGCTGATTCTGGAc                                             <  1:7693012/90‑1 (MQ=255)
                                             aaCGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACa                                            <  1:1113109/93‑1 (MQ=255)
                                             aaCGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACa                                            >  2:9403255/1‑93 (MQ=255)
                                             aaCGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACa                                            >  1:7040264/1‑93 (MQ=255)
                                              aCGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGCGCAGCACGGGAATGGCTGATTCTGAACAt                                           >  1:8391144/1‑93 (MQ=255)
                                              aCGGCCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACAt                                           <  2:2668695/93‑1 (MQ=255)
                                                 ggCCCTGAAAACCACGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGAAGAACGGGAATGGCTGATTCTGAACATGGc                                        <  2:7683622/93‑1 (MQ=255)
                                                   cccTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACCTGGCgg                                      >  1:6939294/1‑93 (MQ=255)
                                                   cccTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCgg                                      <  1:4297250/93‑1 (MQ=255)
                                                       gAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACt                                  >  1:2599757/1‑93 (MQ=255)
                                                        aaaaCCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTg                                 >  1:5167344/1‑93 (MQ=255)
                                                         aaccccTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTgg                                <  2:187002/90‑1 (MQ=255)
                                                         aaaCCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTgg                                <  1:1639714/93‑1 (MQ=255)
                                                         aaaCCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTgg                                <  2:5802557/93‑1 (MQ=255)
                                                           aCCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAg                              <  1:6067804/93‑1 (MQ=255)
                                                              cTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCgg                           >  2:5309993/1‑92 (MQ=255)
                                                              cTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGt                           >  2:3239860/1‑93 (MQ=255)
                                                              cTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGt                           <  2:4506689/93‑1 (MQ=255)
                                                               tGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTg                          >  2:7836012/1‑93 (MQ=255)
                                                                gggccGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTga                         <  1:4895666/90‑1 (MQ=255)
                                                                ggACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTga                         <  2:8559409/93‑1 (MQ=255)
                                                                ggACCGCCATCTTCAAGAACCCGGCGCGGCCTTCACCCTCACCCGTGCAGCACGGGGATGGCTGAATCCGGACATGGCGGGACTGGAGCGTga                         <  2:3389612/93‑1 (MQ=255)
                                                                 gACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTgaa                        <  1:5901743/93‑1 (MQ=255)
                                                                 gACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTgaa                        >  1:9895515/1‑93 (MQ=255)
                                                                        aTCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTcc                 <  1:4026649/93‑1 (MQ=255)
                                                                        aTCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTcc                 <  2:9292283/93‑1 (MQ=255)
                                                                         tCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCg                <  2:4820910/93‑1 (MQ=255)
                                                                         tCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCg                >  1:977139/1‑93 (MQ=255)
                                                                         tCTTCATGAACCCGGCGCGGCCTCCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCg                <  2:7230301/93‑1 (MQ=255)
                                                                          cTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCgg               <  1:3748047/93‑1 (MQ=255)
                                                                           ttCATGAACCCGGCGCGTCCTTCACGTCCACCCGTGCAGCACGGTAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCggg              <  2:6945457/93‑1 (MQ=38)
                                                                           ttCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGCCTGGAGAGTGAAGGATTCCggg              >  2:8154662/1‑93 (MQ=255)
                                                                            tCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGt             <  1:4605411/93‑1 (MQ=255)
                                                                              aTGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGc           >  2:36046/1‑93 (MQ=255)
                                                                              aTGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGGGAAGAATTCCGGGGGc           >  1:2435188/1‑93 (MQ=255)
                                                                               tGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGCAAGTGAAGCATTCCGGGTGCt          >  2:1808488/1‑93 (MQ=255)
                                                                               tGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGGGAAGAATTCCGGGTGCt          >  2:2540041/1‑93 (MQ=255)
                                                                                  aCCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTa       <  1:617113/93‑1 (MQ=255)
                                                                                   cccGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTAt      <  1:392431/93‑1 (MQ=255)
                                                                                    ccGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATc     >  1:6651432/1‑93 (MQ=255)
                                                                                    ccGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATc     >  1:1812886/1‑93 (MQ=255)
                                                                                    ccGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATc     >  2:5371156/1‑93 (MQ=255)
                                                                                       gcgcGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGGGCTGGATCTGa  >  1:5080923/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGA  >  NC_000913/2074313‑2074492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAACAA  >  NC_000913/2074310‑2074496
                                                                                             |                                                                                             
GAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGC                                                                                               <  SRR4302540.314391/94‑1 (MQ=60)
 AAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCG                                                                                              >  SRR4302540.8380674/1‑94 (MQ=60)
   GGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGC                                                                                            >  SRR4302540.7813562/1‑94 (MQ=60)
       AAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTC                                                                                        <  SRR4302540.2950491/94‑1 (MQ=60)
       AAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTC                                                                                        <  SRR4302540.5926277/94‑1 (MQ=60)
        AGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCA                                                                                       >  SRR4302540.6722104/1‑94 (MQ=60)
         GTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCAC                                                                                      <  SRR4302540.4971856/94‑1 (MQ=60)
         GTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCAC                                                                                      <  SRR4302540.9663157/94‑1 (MQ=60)
           AGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCT                                                                                    >  SRR4302540.3413553/1‑94 (MQ=60)
           AGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCT                                                                                    >  SRR4302540.719555/1‑94 (MQ=60)
           AGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCC                                                                                    >  SRR4302540.719556/1‑94 (MQ=60)
             AGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCC                                                                                  >  SRR4302540.2551737/1‑94 (MQ=60)
               GAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCAC                                                                                <  SRR4302540.9636069/94‑1 (MQ=60)
                AGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACC                                                                               >  SRR4302540.7464745/1‑94 (MQ=60)
                AGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACC                                                                               >  SRR4302540.2531371/1‑94 (MQ=60)
                  AGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCG                                                                             >  SRR4302540.8819949/1‑94 (MQ=60)
                    ATGGCGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAATCCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTG                                                                           <  SRR4302540.8564743/94‑1 (MQ=60)
                    ATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGTCTTCACCTCCACCCGTG                                                                           >  SRR4302540.9077051/1‑94 (MQ=60)
                    ATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTG                                                                           >  SRR4302540.4659794/1‑94 (MQ=60)
                      GACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCA                                                                         <  SRR4302540.2045828/94‑1 (MQ=60)
                      GACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCA                                                                         <  SRR4302540.1690167/94‑1 (MQ=60)
                      GACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCA                                                                         <  SRR4302540.6402422/94‑1 (MQ=60)
                      GACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCA                                                                         <  SRR4302540.2977038/94‑1 (MQ=60)
                      GACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCA                                                                         <  SRR4302540.6793790/94‑1 (MQ=60)
                         GCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCA                                                                      <  SRR4302540.5081715/94‑1 (MQ=60)
                          CCCGGCGAGCGTAGCCTCATTCAACGGGCCCTTAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCAC                                                                     <  SRR4302540.5291061/94‑1 (MQ=60)
                          CCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCAC                                                                     <  SRR4302540.1271722/94‑1 (MQ=60)
                          CCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCAC                                                                     <  SRR4302540.145109/94‑1 (MQ=60)
                          CCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCAC                                                                     >  SRR4302540.3166308/1‑94 (MQ=60)
                          CCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCAC                                                                     <  SRR4302540.982929/94‑1 (MQ=60)
                           CCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACG                                                                    >  SRR4302540.1101080/1‑94 (MQ=60)
                            ccgcgcgcggcGCCTCATTCAACGCGCCCTGAAAACCCTGGACCCCCATCCTCACGAACCCGGCGCGGCCTTCACCTCCCCCCGTGCAGCACGG                                                                   <  SRR4302540.1368326/83‑1 (MQ=60)
                             GGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGG                                                                  <  SRR4302540.5553356/94‑1 (MQ=60)
                                GAGCGCAGCCTCATTCAACGGGCCCTGAAAGCCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAAT                                                               <  SRR4302540.8840163/94‑1 (MQ=60)
                                GAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAAT                                                               <  SRR4302540.7937927/94‑1 (MQ=60)
                                GAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAAT                                                               <  SRR4302540.2741392/94‑1 (MQ=60)
                                GAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAAT                                                               <  SRR4302540.1473940/94‑1 (MQ=60)
                                GAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAAT                                                               >  SRR4302540.9842205/1‑94 (MQ=60)
                                 GGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATG                                                              <  SRR4302540.5840925/94‑1 (MQ=60)
                                 AGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATG                                                              <  SRR4302540.5179316/94‑1 (MQ=60)
                                  GCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGG                                                             <  SRR4302540.8563048/94‑1 (MQ=60)
                                   CGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGC                                                            >  SRR4302540.1661887/1‑94 (MQ=60)
                                    GCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCT                                                           >  SRR4302540.285389/1‑94 (MQ=60)
                                    GCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCT                                                           <  SRR4302540.8329365/94‑1 (MQ=60)
                                     CAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTG                                                          <  SRR4302540.5514526/94‑1 (MQ=60)
                                     CAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTG                                                          <  SRR4302540.4509250/94‑1 (MQ=60)
                                      AGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGA                                                         >  SRR4302540.7923150/1‑94 (MQ=60)
                                      AGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGA                                                         >  SRR4302540.1998390/1‑94 (MQ=60)
                                       GCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGAT                                                        <  SRR4302540.2463240/94‑1 (MQ=60)
                                        CCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATT                                                       <  SRR4302540.9905531/94‑1 (MQ=60)
                                         CTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTC                                                      <  SRR4302540.9518424/94‑1 (MQ=60)
                                         CTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTC                                                      >  SRR4302540.10030660/1‑94 (MQ=60)
                                         CTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTC                                                      >  SRR4302540.10030661/1‑94 (MQ=60)
                                         CTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTC                                                      >  SRR4302540.9616034/1‑94 (MQ=60)
                                         CTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTC                                                      >  SRR4302540.9689222/1‑94 (MQ=60)
                                          TCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCT                                                     >  SRR4302540.7650029/1‑94 (MQ=60)
                                           CATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTG                                                    <  SRR4302540.5290701/94‑1 (MQ=60)
                                            ATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGA                                                   <  SRR4302540.107671/94‑1 (MQ=60)
                                            ATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGA                                                   >  SRR4302540.7328505/1‑94 (MQ=60)
                                             TTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTTAA                                                  >  SRR4302540.9347928/1‑94 (MQ=60)
                                             TTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAA                                                  >  SRR4302540.5057144/1‑94 (MQ=60)
                                              TCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAAC                                                 >  SRR4302540.4441158/1‑94 (MQ=60)
                                              TCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAAC                                                 <  SRR4302540.8991451/94‑1 (MQ=60)
                                               CAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACA                                                <  SRR4302540.7780567/94‑1 (MQ=60)
                                               CAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACA                                                >  SRR4302540.7118539/1‑94 (MQ=60)
                                                AACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCCCCCGTGCAGCACGGGAATGGCTGATTCTGAACAT                                               <  SRR4302540.8647910/94‑1 (MQ=60)
                                                AACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACAT                                               <  SRR4302540.1124640/94‑1 (MQ=60)
                                                AACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGCGCAGCACGGGAATGGCTGATTCTGAACAT                                               >  SRR4302540.8488514/1‑94 (MQ=60)
                                                 ACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATG                                              >  SRR4302540.9711680/1‑94 (MQ=60)
                                                 ACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATG                                              <  SRR4302540.9743637/94‑1 (MQ=60)
                                                    GGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCG                                           <  SRR4302540.8043253/94‑1 (MQ=60)
                                                     GCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACCTGGCGG                                          >  SRR4302540.7016533/1‑94 (MQ=60)
                                                      CCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGG                                         <  SRR4302540.6311908/94‑1 (MQ=60)
                                                      CCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGG                                         <  SRR4302540.4034848/94‑1 (MQ=60)
                                                      CCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGG                                         <  SRR4302540.650828/94‑1 (MQ=60)
                                                      CCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGG                                         <  SRR4302540.4344016/94‑1 (MQ=60)
                                                       CCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGA                                        >  SRR4302540.5413900/1‑94 (MQ=60)
                                                         TGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACT                                      >  SRR4302540.2627788/1‑94 (MQ=60)
                                                          GAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTG                                     >  SRR4302540.5223548/1‑94 (MQ=60)
                                                           AAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGG                                    >  SRR4302540.7421402/1‑94 (MQ=60)
                                                            AAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGA                                   <  SRR4302540.1657635/94‑1 (MQ=60)
                                                              ACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGC                                 <  SRR4302540.6135267/94‑1 (MQ=60)
                                                               CCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCG                                <  SRR4302540.3890027/94‑1 (MQ=60)
                                                                CCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGG                               >  SRR4302540.2776091/1‑94 (MQ=60)
                                                                 CTGGACCTCCATCTTCATGAACCCGGCGCGGCCTTCACCTACACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTG                              <  SRR4302540.9445713/94‑1 (MQ=60)
                                                                 CTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTG                              <  SRR4302540.4048435/94‑1 (MQ=60)
                                                                 CTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTG                              <  SRR4302540.9197645/94‑1 (MQ=60)
                                                                   GGGCCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAA                            <  SRR4302540.4948320/94‑1 (MQ=60)
                                                                   GGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAA                            >  SRR4302540.10011076/1‑94 (MQ=60)
                                                                    GACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGGGTGAAG                           <  SRR4302540.5967210/94‑1 (MQ=60)
                                                                    GACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAG                           <  SRR4302540.5966915/94‑1 (MQ=60)
                                                                       CGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAAT                        <  SRR4302540.6165461/94‑1 (MQ=60)
                                                                       CGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAAT                        >  SRR4302540.1961376/1‑94 (MQ=60)
                                                                          CATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCC                     >  SRR4302540.4598109/1‑94 (MQ=60)
                                                                           ATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCG                    <  SRR4302540.4070072/94‑1 (MQ=60)
                                                                           ATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGTACATGGCGGGACcgttgcgcgttgaattccg                    >  SRR4302540.4467681/1‑75 (MQ=60)
                                                                           ATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCG                    >  SRR4302540.987162/1‑94 (MQ=60)
                                                                           ATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCG                    >  SRR4302540.1173445/1‑94 (MQ=60)
                                                                           ATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCG                    >  SRR4302540.582123/1‑94 (MQ=60)
                                                                           ATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCG                    >  SRR4302540.1285800/1‑94 (MQ=60)
                                                                           ATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCG                    >  SRR4302540.8482396/1‑94 (MQ=60)
                                                                           ATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGAGTGAAGAATTGCG                    >  SRR4302540.4108970/1‑94 (MQ=60)
                                                                             CTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGG                  <  SRR4302540.3788944/94‑1 (MQ=60)
                                                                               TCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTG                <  SRR4302540.4654878/94‑1 (MQ=60)
                                                                               TCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTG                >  SRR4302540.7618588/1‑94 (MQ=60)
                                                                                CATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGGGAAGAATTCCGGGGGC               >  SRR4302540.2461420/1‑94 (MQ=60)
                                                                                   GAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTTCGGGTGCTGT            >  SRR4302540.7093650/1‑94 (MQ=60)
                                                                                    AACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTA           >  SRR4302540.5051793/1‑94 (MQ=60)
                                                                                     ACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTAT          <  SRR4302540.623694/94‑1 (MQ=60)
                                                                                      CCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATC         >  SRR4302540.6725006/1‑94 (MQ=60)
                                                                                      CCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATC         >  SRR4302540.1832674/1‑94 (MQ=60)
                                                                                      CCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATC         <  SRR4302540.396494/94‑1 (MQ=60)
                                                                                       CCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCT        <  SRR4302540.6337667/94‑1 (MQ=60)
                                                                                       CCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCT        <  SRR4302540.6724155/94‑1 (MQ=60)
                                                                                         GGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGA      <  SRR4302540.3060995/94‑1 (MQ=60)
                                                                                         GGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGGGCTGGATCTGA      >  SRR4302540.5135857/1‑94 (MQ=60)
                                                                                           CGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAAC    <  SRR4302540.8967125/94‑1 (MQ=60)
                                                                                             CGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAACAA  <  SRR4302540.2781250/94‑1 (MQ=60)
                                                                                             |                                                                                             
GAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAACAA  >  NC_000913/2074310‑2074496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑