Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,075,662 A→G I24M (ATA→ATG)  yeeV → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,075,6620AG99.2% 847.8 / ‑3.6 264I24M (ATA→ATG) yeeVhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (133/129);  total (134/130)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

Only 100 of 278 total aligned reads displayed.

TATCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGA  >  NC_000913/2075572‑2075751
                                                                                          |                                                                                         
tatCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGct                                                                                         >  2:6693530/1‑93 (MQ=255)
tatCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGct                                                                                         >  2:10011094/1‑93 (MQ=255)
tatCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGct                                                                                         >  1:6006504/1‑93 (MQ=255)
tatCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGct                                                                                         >  1:3299400/1‑93 (MQ=255)
 atCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGctg                                                                                        >  1:2123681/1‑93 (MQ=255)
 atCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGctg                                                                                        >  2:7852310/1‑93 (MQ=255)
  tCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGAtgctgc                                                                                       <  2:3043050/93‑1 (MQ=255)
  tCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGAtgctgc                                                                                       >  2:2483513/1‑93 (MQ=255)
   cAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATgctgct                                                                                      <  1:3602513/93‑1 (MQ=255)
    aGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGctgctg                                                                                     <  2:1428815/93‑1 (MQ=255)
       aaaaTCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTcc                                                                                  <  2:7468168/93‑1 (MQ=255)
       aaaaTCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTcc                                                                                  >  2:1913944/1‑93 (MQ=255)
        aaaTCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTccc                                                                                 <  2:8877275/93‑1 (MQ=255)
         aaTCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCg                                                                                >  2:9699987/1‑93 (MQ=255)
          aTCTTCAGTATGAAAGCATTACCTGTATGACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGa                                                                               <  1:6473168/93‑1 (MQ=255)
          aTCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGa                                                                               <  1:2691654/93‑1 (MQ=255)
          aTCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGa                                                                               >  2:3415994/1‑93 (MQ=255)
           tCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGAc                                                                              <  1:6330119/93‑1 (MQ=255)
             ttCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGActg                                                                            >  2:1106936/1‑93 (MQ=255)
              tCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGActgc                                                                           >  2:1272785/1‑93 (MQ=255)
              tCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGActgc                                                                           >  2:8579506/1‑93 (MQ=255)
                aGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGActgctg                                                                         <  1:8104466/93‑1 (MQ=255)
                aGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGActgctg                                                                         >  2:6514255/1‑93 (MQ=255)
                  tATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCGCGACTGCTGGa                                                                       >  2:6018556/1‑93 (MQ=255)
                  tATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGa                                                                       <  2:7347911/93‑1 (MQ=255)
                    tGAAAACATTATCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCGGGAcc                                                                     >  1:5007590/1‑93 (MQ=255)
                    tGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGAcc                                                                     >  1:2889307/1‑93 (MQ=255)
                    tGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGAcc                                                                     >  2:7429001/1‑93 (MQ=255)
                     gAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCa                                                                    >  1:3521976/1‑93 (MQ=255)
                      aaaaCATTACCTGTATTACCCGGGCTGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAg                                                                   <  2:4922105/93‑1 (MQ=255)
                      aaaaCATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAg                                                                   <  1:5264668/93‑1 (MQ=255)
                       aaaCATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGc                                                                  <  2:3152328/93‑1 (MQ=255)
                       aaaCATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGc                                                                  <  2:7976254/93‑1 (MQ=255)
                       aaaCATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGc                                                                  <  2:1572294/93‑1 (MQ=255)
                        aaCATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCa                                                                 <  1:8642499/93‑1 (MQ=255)
                          cATTACTTGTATTACACGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGATGCTGTCCCGACTGCTGGAGCAGCACt                                                               >  2:346798/1‑93 (MQ=255)
                          cATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACt                                                               >  2:6789174/1‑93 (MQ=255)
                          cATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACt                                                               >  2:6956920/1‑93 (MQ=255)
                           aTTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTa                                                              <  2:6456401/93‑1 (MQ=255)
                           aTTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTa                                                              <  1:1698870/93‑1 (MQ=255)
                           aTTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTa                                                              <  2:9128507/93‑1 (MQ=255)
                            ttACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTAt                                                             <  2:8201682/93‑1 (MQ=255)
                             tACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATg                                                            <  2:7639269/93‑1 (MQ=255)
                             tACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATg                                                            >  1:4337129/1‑93 (MQ=255)
                                cTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGcc                                                         >  1:8894955/1‑93 (MQ=255)
                                 tGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCt                                                        >  1:6337774/1‑93 (MQ=255)
                                  gTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTc                                                       >  1:449692/1‑93 (MQ=255)
                                   tATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTca                                                      <  1:1002130/93‑1 (MQ=255)
                                     ttACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTcaca                                                    <  1:9853654/93‑1 (MQ=255)
                                       aCCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACt                                                  >  1:3437349/1‑93 (MQ=255)
                                       aCCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACt                                                  <  1:5738498/93‑1 (MQ=255)
                                       aCCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACt                                                  >  2:1670015/1‑93 (MQ=255)
                                        cccGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTg                                                 >  1:9467460/1‑93 (MQ=255)
                                        cccGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTg                                                 >  2:5770750/1‑93 (MQ=255)
                                         ccGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGa                                                <  2:9503376/93‑1 (MQ=255)
                                         ccGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGa                                                >  2:9306354/1‑93 (MQ=255)
                                          cGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGaa                                               <  1:8335680/93‑1 (MQ=255)
                                          cGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGaa                                               >  1:7458239/1‑93 (MQ=255)
                                          cGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGaa                                               <  2:8286619/93‑1 (MQ=255)
                                                ggcggcCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGacac                                         >  2:259904/1‑93 (MQ=255)
                                                ggcggcCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGacac                                         >  2:5326940/1‑93 (MQ=255)
                                                  cggcCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGacacac                                       <  1:4075181/93‑1 (MQ=255)
                                                   ggcCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACAcc                                      <  2:3823172/93‑1 (MQ=255)
                                                    gcCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCt                                     >  1:4785831/1‑93 (MQ=255)
                                                    gcCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCt                                     <  1:7021650/93‑1 (MQ=255)
                                                    gcCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCt                                     >  1:6199196/1‑93 (MQ=255)
                                                     ccAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCtt                                    >  2:27430/1‑93 (MQ=255)
                                                     ccAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCtt                                    <  2:56485/93‑1 (MQ=255)
                                                      cAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCttt                                   >  1:8972577/1‑93 (MQ=255)
                                                      cAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCttt                                   <  2:4224993/93‑1 (MQ=255)
                                                       aGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCtttt                                  <  2:6248417/93‑1 (MQ=255)
                                                        gTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTg                                 >  2:3676098/1‑93 (MQ=255)
                                                        gTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTg                                 >  1:4569303/1‑93 (MQ=255)
                                                         ttCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGc                                >  1:6740693/1‑93 (MQ=255)
                                                         ttCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGc                                >  1:4906233/1‑93 (MQ=255)
                                                          tctcGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGcc                               >  1:263471/1‑93 (MQ=255)
                                                           ctcGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCg                              <  1:5611288/93‑1 (MQ=255)
                                                              gCCCGTCTCCTGTTGAAATCTGGGAGATGCTGCTGTCCCGACTTCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATg                           <  1:7591084/93‑1 (MQ=255)
                                                              gCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATg                           >  1:4198524/1‑93 (MQ=255)
                                                               cccGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGa                          >  2:4574658/1‑93 (MQ=255)
                                                               cccGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGa                          >  2:7244779/1‑93 (MQ=255)
                                                                ccGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGaa                         >  1:226175/1‑93 (MQ=255)
                                                                ccGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGaa                         <  2:5149519/93‑1 (MQ=255)
                                                                ccGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGaa                         <  1:2202272/93‑1 (MQ=255)
                                                                ccGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGaa                         >  1:2976274/1‑93 (MQ=255)
                                                                 cGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAAc                        <  1:877846/93‑1 (MQ=255)
                                                                   tctcCTGTTGAGATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACgt                      <  2:8953166/93‑1 (MQ=255)
                                                                      ccTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGtgtg                   <  2:2944372/93‑1 (MQ=255)
                                                                       cTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGa                  <  1:3943416/93‑1 (MQ=255)
                                                                       cTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGa                  <  1:2454349/93‑1 (MQ=255)
                                                                        tGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGAt                 >  1:7181938/1‑93 (MQ=255)
                                                                         gTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGAtt                <  1:3859533/93‑1 (MQ=255)
                                                                         gTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGAtt                <  1:5524802/93‑1 (MQ=255)
                                                                          ttGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTg               <  1:5168440/93‑1 (MQ=255)
                                                                            gAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTAAGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGag             <  2:2346334/93‑1 (MQ=255)
                                                                             aaaTCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGagc            <  1:926762/93‑1 (MQ=255)
                                                                              aaTCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGagca           >  1:1248783/1‑93 (MQ=255)
                                                                                  tGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCat       >  1:7232880/1‑93 (MQ=255)
                                                                                    gCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCatat     <  1:7734802/93‑1 (MQ=255)
                                                                                      aGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTg   <  2:2709501/93‑1 (MQ=255)
                                                                                          |                                                                                         
TATCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGA  >  NC_000913/2075572‑2075751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCATATCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGA  >  NC_000913/2075569‑2075751
                                                                                             |                                                                                         
GCATATCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATG                                                                                           <  SRR4302540.3717345/94‑1 (MQ=60)
 CATATCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGC                                                                                          >  SRR4302540.1424105/1‑94 (MQ=60)
  ATATCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCT                                                                                         <  SRR4302540.4051492/94‑1 (MQ=60)
  ATATCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCT                                                                                         >  SRR4302540.6073097/1‑94 (MQ=60)
  ATATCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCT                                                                                         >  SRR4302540.3335515/1‑94 (MQ=60)
  ATATCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCT                                                                                         <  SRR4302540.6173180/94‑1 (MQ=60)
   TATCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTG                                                                                        <  SRR4302540.2197912/94‑1 (MQ=60)
   TATCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTG                                                                                        >  SRR4302540.2146462/1‑94 (MQ=60)
      CAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTG                                                                                     <  SRR4302540.8492016/94‑1 (MQ=60)
      CAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTG                                                                                     <  SRR4302540.3641881/94‑1 (MQ=60)
       AGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGT                                                                                    <  SRR4302540.2610033/94‑1 (MQ=60)
       AGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGT                                                                                    >  SRR4302540.2972642/1‑94 (MQ=60)
         GAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCC                                                                                  >  SRR4302540.9594528/1‑94 (MQ=60)
             ATCTTCAGTATGAAAGCATTACCTGTATGACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGAC                                                                              <  SRR4302540.6545192/94‑1 (MQ=60)
             ATCTTCAGTATGAAAACATTACCTGTATTCCCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGAC                                                                              <  SRR4302540.4458221/94‑1 (MQ=60)
             ATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGAC                                                                              >  SRR4302540.3655679/1‑94 (MQ=60)
             ATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGAC                                                                              <  SRR4302540.2720729/94‑1 (MQ=60)
             ATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGAC                                                                              <  SRR4302540.4610198/94‑1 (MQ=60)
              TCTTCAGTATGAAAACATTACCTGTATTCCCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACT                                                                             <  SRR4302540.6400612/94‑1 (MQ=60)
              TCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACT                                                                             <  SRR4302540.9012245/94‑1 (MQ=60)
              TCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACT                                                                             <  SRR4302540.6400611/94‑1 (MQ=60)
               CTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTG                                                                            <  SRR4302540.3133335/94‑1 (MQ=60)
                TTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGC                                                                           >  SRR4302540.8222744/1‑94 (MQ=60)
                 TCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCT                                                                          >  SRR4302540.5291122/1‑94 (MQ=60)
                 TCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCT                                                                          <  SRR4302540.4320895/94‑1 (MQ=60)
                  CAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTG                                                                         <  SRR4302540.4180265/94‑1 (MQ=60)
                  CAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTG                                                                         <  SRR4302540.7279223/94‑1 (MQ=60)
                  CAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTG                                                                         >  SRR4302540.6288337/1‑94 (MQ=60)
                   AGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGG                                                                        <  SRR4302540.8198530/94‑1 (MQ=60)
                      ATGAAAACATTATCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCGGGACC                                                                     >  SRR4302540.5061822/1‑94 (MQ=60)
                      ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACC                                                                     >  SRR4302540.2920778/1‑94 (MQ=60)
                      ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACC                                                                     >  SRR4302540.5061821/1‑94 (MQ=60)
                      ATGAAAACATGTCCTGTATTTCTCGGGCAGGCGGGCAGTGCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACC                                                                     <  SRR4302540.4532548/94‑1 (MQ=60)
                       TGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCA                                                                    >  SRR4302540.3560364/1‑94 (MQ=60)
                       TGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCA                                                                    >  SRR4302540.10110810/1‑94 (MQ=60)
                       TGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCA                                                                    <  SRR4302540.5345206/94‑1 (MQ=60)
                         AAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGC                                                                  <  SRR4302540.5322120/94‑1 (MQ=60)
                           AACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCAC                                                                <  SRR4302540.8742893/94‑1 (MQ=60)
                           AACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCAC                                                                <  SRR4302540.8742891/94‑1 (MQ=60)
                            ACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACT                                                               >  SRR4302540.9491403/1‑94 (MQ=60)
                             CATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTA                                                              >  SRR4302540.5632297/1‑94 (MQ=60)
                              ATTACCTTTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTAT                                                             <  SRR4302540.4007737/94‑1 (MQ=60)
                              ATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTAT                                                             <  SRR4302540.6171159/94‑1 (MQ=60)
                              ATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTAT                                                             >  SRR4302540.5222364/1‑94 (MQ=60)
                              ATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTAT                                                             >  SRR4302540.92132/1‑94 (MQ=60)
                              ATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTAT                                                             <  SRR4302540.1717270/94‑1 (MQ=60)
                              ATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTAT                                                             >  SRR4302540.3581135/1‑94 (MQ=60)
                              ATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTAT                                                             <  SRR4302540.5819748/94‑1 (MQ=60)
                              ATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTAT                                                             <  SRR4302540.4948012/94‑1 (MQ=60)
                              ATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGGCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTAT                                                             <  SRR4302540.5115590/94‑1 (MQ=60)
                               TTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATG                                                            >  SRR4302540.4384346/1‑94 (MQ=60)
                               TTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATG                                                            <  SRR4302540.8804701/94‑1 (MQ=60)
                               TTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATG                                                            >  SRR4302540.8149227/1‑94 (MQ=60)
                                  CCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCC                                                         >  SRR4302540.7686469/1‑94 (MQ=60)
                                  CCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCC                                                         >  SRR4302540.8997916/1‑94 (MQ=60)
                                   CTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCT                                                        <  SRR4302540.4562680/94‑1 (MQ=60)
                                   CTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCT                                                        >  SRR4302540.6408351/1‑94 (MQ=60)
                                   CTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCT                                                        >  SRR4302540.6408352/1‑94 (MQ=60)
                                    TGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTC                                                       >  SRR4302540.3475711/1‑94 (MQ=60)
                                    TGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTC                                                       >  SRR4302540.454485/1‑94 (MQ=60)
                                      TATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCAC                                                     <  SRR4302540.1012310/94‑1 (MQ=60)
                                      TATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCAC                                                     <  SRR4302540.6114759/94‑1 (MQ=60)
                                      TATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCAC                                                     >  SRR4302540.7653510/1‑94 (MQ=60)
                                       ATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACA                                                    <  SRR4302540.9035830/94‑1 (MQ=60)
                                       ATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACA                                                    >  SRR4302540.2489764/1‑94 (MQ=60)
                                        TTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACAC                                                   <  SRR4302540.9968812/94‑1 (MQ=60)
                                        TTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACAC                                                   >  SRR4302540.5790494/1‑94 (MQ=60)
                                         TACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTNGNNCAGCACTATGNCNTCACNCN                                                  >  SRR4302540.1914998/1‑94 (MQ=60)
                                         TACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACT                                                  >  SRR4302540.4725168/1‑94 (MQ=60)
                                         TACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACT                                                  >  SRR4302540.3474715/1‑94 (MQ=60)
                                         TACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACT                                                  <  SRR4302540.5225760/94‑1 (MQ=60)
                                         TACCCGGGCAGGCGGCCAGTTCTCGACCGTCTCCTGTTCAAATCTGGCAGATCCTCCTGTCCCacatgctggcccagcatcatggcctcccaca                                                  >  SRR4302540.3474714/1‑63 (MQ=58)
                                          ACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTG                                                 >  SRR4302540.9579770/1‑94 (MQ=60)
                                          ACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTG                                                 >  SRR4302540.9579769/1‑94 (MQ=60)
                                          ACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTG                                                 >  SRR4302540.5991600/1‑94 (MQ=60)
                                          ACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTG                                                 <  SRR4302540.5801747/94‑1 (MQ=60)
                                           CCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGA                                                <  SRR4302540.2232930/94‑1 (MQ=60)
                                           CCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGA                                                <  SRR4302540.6419779/94‑1 (MQ=60)
                                           CCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGA                                                <  SRR4302540.1524194/94‑1 (MQ=60)
                                            CCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAA                                               >  SRR4302540.7541391/1‑94 (MQ=60)
                                             CGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAAT                                              <  SRR4302540.7205021/94‑1 (MQ=60)
                                             CGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAAT                                              <  SRR4302540.8432436/94‑1 (MQ=60)
                                              GGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATG                                             <  SRR4302540.1243845/94‑1 (MQ=60)
                                              GGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATG                                             >  SRR4302540.3089390/1‑94 (MQ=60)
                                                  AGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACAC                                         <  SRR4302540.3693143/94‑1 (MQ=60)
                                                  AGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACAC                                         >  SRR4302540.2689889/1‑94 (MQ=60)
                                                   GGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACA                                        <  SRR4302540.3357588/94‑1 (MQ=60)
                                                    GCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACAC                                       <  SRR4302540.2681773/94‑1 (MQ=60)
                                                     CGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACC                                      <  SRR4302540.4119107/94‑1 (MQ=60)
                                                      GGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCT                                     >  SRR4302540.4836999/1‑94 (MQ=60)
                                                      GGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCT                                     >  SRR4302540.6268203/1‑94 (MQ=60)
                                                       GCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTT                                    <  SRR4302540.7099641/94‑1 (MQ=60)
                                                        CCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTT                                   >  SRR4302540.9079173/1‑94 (MQ=60)
                                                        CCAGTGCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTT                                   <  SRR4302540.8418471/94‑1 (MQ=60)
                                                         CAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTT                                  <  SRR4302540.9610931/94‑1 (MQ=60)
                                                          AGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTG                                 >  SRR4302540.4618628/1‑94 (MQ=60)
                                                          AGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTG                                 >  SRR4302540.8731206/1‑94 (MQ=60)
                                                          AGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTG                                 >  SRR4302540.695118/1‑94 (MQ=60)
                                                          AGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTG                                 >  SRR4302540.5730639/1‑94 (MQ=60)
                                                           GTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGC                                >  SRR4302540.6815152/1‑94 (MQ=60)
                                                           GTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGC                                >  SRR4302540.278710/1‑94 (MQ=60)
                                                           GTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGC                                >  SRR4302540.4959041/1‑94 (MQ=60)
                                                           GTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGC                                >  SRR4302540.2198844/1‑94 (MQ=60)
                                                           GTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGC                                >  SRR4302540.4959347/1‑94 (MQ=60)
                                                            TTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCC                               >  SRR4302540.266040/1‑94 (MQ=60)
                                                              CTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGA                             <  SRR4302540.5673017/94‑1 (MQ=60)
                                                              CTCGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGA                             >  SRR4302540.9189314/1‑94 (MQ=60)
                                                                CGCCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATG                           >  SRR4302540.4244202/1‑94 (MQ=60)
                                                                 GCCCGTCTCCTGTTGAAATCTGGGAGATGCTGCTGTCCCGACTTCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGA                          <  SRR4302540.7676349/94‑1 (MQ=60)
                                                                  CCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAA                         >  SRR4302540.3974445/1‑94 (MQ=60)
                                                                  CCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAA                         >  SRR4302540.3009167/1‑94 (MQ=60)
                                                                  CCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAA                         <  SRR4302540.651213/94‑1 (MQ=60)
                                                                  CCCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAA                         >  SRR4302540.228361/1‑94 (MQ=60)
                                                                   CCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAAC                        >  SRR4302540.2032218/1‑94 (MQ=60)
                                                                   CCGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAAC                        <  SRR4302540.2226061/94‑1 (MQ=60)
                                                                    CGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACG                       >  SRR4302540.4831649/1‑94 (MQ=60)
                                                                    CGTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACG                       <  SRR4302540.887163/94‑1 (MQ=60)
                                                                     GTCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGG                      >  SRR4302540.6589585/1‑94 (MQ=60)
                                                                      TCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTG                     >  SRR4302540.156182/1‑94 (MQ=60)
                                                                      TCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTG                     <  SRR4302540.8802749/94‑1 (MQ=60)
                                                                      TCTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTG                     >  SRR4302540.349246/1‑94 (MQ=60)
                                                                       CTCCTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGT                    <  SRR4302540.7911046/94‑1 (MQ=60)
                                                                          CTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTTGACCAGCACTATGGCCTCACCCTGAATGACACTCCTTTTGCCGATGAACGTGTGAT                 <  SRR4302540.2409799/94‑1 (MQ=60)
                                                                          CTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGAT                 >  SRR4302540.7262005/1‑94 (MQ=60)
                                                                          CTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGAT                 <  SRR4302540.2480767/94‑1 (MQ=60)
                                                                          CTGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGAT                 <  SRR4302540.3986009/94‑1 (MQ=60)
                                                                           TGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATT                <  SRR4302540.5465329/94‑1 (MQ=60)
                                                                           TGTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATT                >  SRR4302540.1985875/1‑94 (MQ=60)
                                                                            GTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTG               <  SRR4302540.3901486/94‑1 (MQ=60)
                                                                            GTTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTG               <  SRR4302540.5585639/94‑1 (MQ=60)
                                                                             TTGAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGA              <  SRR4302540.5224659/94‑1 (MQ=60)
                                                                               GAAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGC            <  SRR4302540.8993837/94‑1 (MQ=60)
                                                                                AAATCTGGCTGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCA           <  SRR4302540.2824826/94‑1 (MQ=60)
                                                                                AAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCA           <  SRR4302540.7328078/94‑1 (MQ=60)
                                                                                AAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCA           >  SRR4302540.7560281/1‑94 (MQ=60)
                                                                                AAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCA           <  SRR4302540.936520/94‑1 (MQ=60)
                                                                                AAATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCA           >  SRR4302540.1261535/1‑94 (MQ=60)
                                                                                 AATCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAG          <  SRR4302540.4220082/94‑1 (MQ=60)
                                                                                   TCTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCA        >  SRR4302540.3961592/1‑94 (MQ=60)
                                                                                    CTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCAT       >  SRR4302540.7349539/1‑94 (MQ=60)
                                                                                    CTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCAT       >  SRR4302540.7313549/1‑94 (MQ=60)
                                                                                    CTGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCAT       >  SRR4302540.7313548/1‑94 (MQ=60)
                                                                                     TGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATA      <  SRR4302540.69339/94‑1 (MQ=60)
                                                                                     TGGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATA      >  SRR4302540.6660012/1‑94 (MQ=60)
                                                                                      GGCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATAT     <  SRR4302540.2478300/94‑1 (MQ=60)
                                                                                       GCAGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATT    <  SRR4302540.7825102/94‑1 (MQ=60)
                                                                                         AGATGCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGA  >  SRR4302540.4042145/1‑94 (MQ=60)
                                                                                             |                                                                                         
GCATATCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGA  >  NC_000913/2075569‑2075751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑