Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,092,192 A→G T235A (ACC→GCC)  b2022 → imidazoleglycerolphosphate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,092,1920AG99.2% 771.9 / ‑3.6 241T235A (ACC→GCC) b2022imidazoleglycerolphosphate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (119/120);  total (121/120)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

Only 100 of 259 total aligned reads displayed.

CTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATACCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAA  >  NC_000913/2092102‑2092281
                                                                                          |                                                                                         
cTGGATCCGATCGCTCCCCACGGCGGTTGCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGcc                                                                                         <  1:313381/93‑1 (MQ=255)
cTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGcc                                                                                         >  1:9102383/1‑93 (MQ=255)
 tGGATCAGATCGCTACCCACGGCTGTTTCCGGATGGAAATCAACGTCAAAGGCGTCCTCTATATCGACGATCACCACACCGTCGAAGATGCCg                                                                                        <  1:3034414/93‑1 (MQ=255)
  ggATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCgg                                                                                       <  2:2365960/93‑1 (MQ=255)
   gATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCACCGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGc                                                                                      >  1:4069762/1‑93 (MQ=255)
   gATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGc                                                                                      <  2:5949331/93‑1 (MQ=255)
    aTCAGATCGCTACCCACGGCGGTTTCCGCATGGCAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATACCGGcc                                                                                     >  1:7606628/1‑93 (MQ=255)
    aTCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGcc                                                                                     <  1:9256413/93‑1 (MQ=255)
    aTCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGcc                                                                                     >  2:4701753/1‑93 (MQ=255)
    aTCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACCATCACCACACCGTCGAAGATGCCgggc                                                                                     >  2:5830773/1‑91 (MQ=255)
     tCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCt                                                                                    >  2:7706170/1‑93 (MQ=255)
     tCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCt                                                                                    >  1:6606200/1‑93 (MQ=255)
     tCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCCTCGAAGATGCCGGccg                                                                                    >  2:2927933/1‑92 (MQ=255)
      cAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTg                                                                                   >  2:1078566/1‑93 (MQ=255)
      cAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTg                                                                                   <  1:3072802/93‑1 (MQ=255)
      cAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTg                                                                                   <  2:9582339/93‑1 (MQ=255)
        gATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGgc                                                                                 >  1:7855472/1‑93 (MQ=255)
          tCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGgcgc                                                                               >  2:277647/1‑93 (MQ=255)
          tCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGgcgc                                                                               >  1:8729845/1‑93 (MQ=255)
          tCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGgcgc                                                                               >  1:5007529/1‑93 (MQ=255)
            gCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTg                                                                             <  2:8494652/93‑1 (MQ=255)
            gCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTg                                                                             <  2:3537214/93‑1 (MQ=255)
             cTACCCACGGCGGTTGCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACTCCGTCGAAGATGCCGGCCTGGCGCTgg                                                                            <  2:5573147/93‑1 (MQ=255)
                cccACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCg                                                                         >  2:4420806/1‑93 (MQ=255)
                 ccACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGa                                                                        <  1:3454408/93‑1 (MQ=255)
                  cACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGaa                                                                       >  2:2072542/1‑93 (MQ=255)
                    cggcggTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGAGGCCTGCCTGGCGCTGGGGGaaag                                                                     >  2:6865887/1‑91 (MQ=255)
                       cggTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCt                                                                  <  2:7883902/93‑1 (MQ=255)
                         gTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTaa                                                                >  2:4483269/1‑93 (MQ=255)
                           ttCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTaaaa                                                              >  1:9647933/1‑93 (MQ=255)
                              cGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATc                                                           >  2:7363768/1‑93 (MQ=255)
                                cATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGc                                                         <  2:6530892/93‑1 (MQ=255)
                                 ctGGAAAACAACGTCAAAGGCGACCTCTATGACGACGCTCACCACACCGTGGAAGATGCCGGCCGGGTGCTGGGCGAAGCGCTAAAAATCGcc                                                        <  1:6898751/92‑1 (MQ=255)
                                 aTGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGcc                                                        <  2:521041/93‑1 (MQ=255)
                                  tGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGccc                                                       >  1:2131513/1‑93 (MQ=255)
                                    gAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTc                                                     <  2:2237112/93‑1 (MQ=255)
                                    gAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTc                                                     <  2:1936263/93‑1 (MQ=255)
                                     aaaTCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCg                                                    <  1:3937905/93‑1 (MQ=255)
                                       aTCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGga                                                  >  1:9847067/1‑93 (MQ=255)
                                         cAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGgaga                                                >  2:1842806/1‑93 (MQ=255)
                                           aCGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGGGAAGCGCTAAAAATCGCCTTCGgagaaa                                              >  2:8239041/1‑91 (MQ=255)
                                            cggcAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACaa                                             <  2:2503748/90‑1 (MQ=255)
                                            cGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGAGAAGCGCTAAAAATCGCCCTCGGAGACaa                                             >  1:1225060/1‑93 (MQ=255)
                                             gTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACaaa                                            <  1:2839154/93‑1 (MQ=255)
                                              tCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAAc                                           <  2:3758334/93‑1 (MQ=255)
                                              tCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAAc                                           >  1:139019/1‑93 (MQ=255)
                                              tCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAAc                                           <  2:2542319/93‑1 (MQ=255)
                                                  aGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCgg                                       >  2:729788/1‑93 (MQ=255)
                                                  aGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCgg                                       <  2:3278169/93‑1 (MQ=255)
                                                   ggCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGt                                      >  2:5248613/1‑93 (MQ=255)
                                                   ggCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGt                                      <  2:9357696/93‑1 (MQ=255)
                                                   ggCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGt                                      >  2:742253/1‑93 (MQ=255)
                                                   ggCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGt                                      >  1:1892621/1‑93 (MQ=255)
                                                   ggCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGt                                      >  1:5296941/1‑93 (MQ=255)
                                                     cGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTAt                                    >  1:4514010/1‑93 (MQ=255)
                                                      gACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTAtt                                   >  2:812251/1‑93 (MQ=255)
                                                      gACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTAtt                                   >  1:229978/1‑93 (MQ=255)
                                                      gACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTAtt                                   <  1:6513967/93‑1 (MQ=255)
                                                       aCCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTAttt                                  <  2:5287792/93‑1 (MQ=255)
                                                       aCCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTAttt                                  <  2:5072020/93‑1 (MQ=255)
                                                       aCCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTAttt                                  >  1:5438414/1‑93 (MQ=255)
                                                       aCCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTAttt                                  <  2:7908488/93‑1 (MQ=255)
                                                         ctctATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGGCCAACGCGGTAtttcg                                >  2:4526110/1‑91 (MQ=255)
                                                         ctctATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGc                                >  1:171521/1‑93 (MQ=255)
                                                             agatCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCt                            <  1:4271493/91‑1 (MQ=255)
                                                              tatCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCtt                           >  1:3116622/1‑93 (MQ=255)
                                                               atCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCttt                          >  1:6779/1‑93 (MQ=255)
                                                               atCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCttt                          <  2:10032537/93‑1 (MQ=255)
                                                               atCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCttt                          <  2:941866/93‑1 (MQ=255)
                                                                 cgacgaTCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTgg                        <  1:1603794/93‑1 (MQ=255)
                                                                  gacgaTCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGt                       <  1:3768566/93‑1 (MQ=255)
                                                                   acgaTCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGtt                      <  1:7792421/93‑1 (MQ=255)
                                                                    cgaTCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGttt                     >  1:7105831/1‑93 (MQ=255)
                                                                      aTCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTtg                   >  1:4719964/1‑93 (MQ=255)
                                                                      aTCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTtg                   >  2:9242414/1‑93 (MQ=255)
                                                                       tCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTtgt                  <  1:5406837/93‑1 (MQ=255)
                                                                       tCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTtgt                  >  1:5869837/1‑93 (MQ=255)
                                                                         cccacACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGc                <  2:4116632/92‑1 (MQ=255)
                                                                           cacaCCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTa              <  2:2297312/93‑1 (MQ=255)
                                                                            acacCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTAc             <  2:5986417/93‑1 (MQ=255)
                                                                            acacCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTAc             >  1:9381984/1‑93 (MQ=255)
                                                                             cacCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTAcc            <  1:2652382/93‑1 (MQ=255)
                                                                               ccGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGa          <  1:7349898/93‑1 (MQ=255)
                                                                                cGTCGAAGATGCCGGCCTTGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGAt         >  2:9834963/1‑93 (MQ=255)
                                                                                 gTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATg        <  2:1540652/93‑1 (MQ=255)
                                                                                 gTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATg        <  1:9159084/93‑1 (MQ=255)
                                                                                 gTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATg        <  1:8335609/93‑1 (MQ=255)
                                                                                 gTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATg        >  1:8039206/1‑93 (MQ=255)
                                                                                 gTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATg        >  1:1080591/1‑93 (MQ=255)
                                                                                  tCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATgg       >  2:6990509/1‑93 (MQ=255)
                                                                                  tCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATgg       <  2:4601708/93‑1 (MQ=255)
                                                                                    gAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGag     >  2:2052016/1‑92 (MQ=255)
                                                                                    gAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGAc     <  1:537603/93‑1 (MQ=255)
                                                                                     aaGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACg    >  2:4953575/1‑93 (MQ=255)
                                                                                     aaGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACg    >  1:978082/1‑93 (MQ=255)
                                                                                      aGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGa   >  2:751667/1‑93 (MQ=255)
                                                                                      aGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGa   <  2:3121936/93‑1 (MQ=255)
                                                                                       gATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGaa  <  2:2618886/93‑1 (MQ=255)
                                                                                       gATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGaa  <  1:10003602/93‑1 (MQ=255)
                                                                                       gATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGaa  <  1:116070/93‑1 (MQ=255)
                                                                                          |                                                                                         
CTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATACCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAA  >  NC_000913/2092102‑2092281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATACCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAATGC  >  NC_000913/2092099‑2092284
                                                                                             |                                                                                            
ATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATG                                                                                              >  SRR4302540.3437072/1‑94 (MQ=60)
  GCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCC                                                                                            <  SRR4302540.4494016/94‑1 (MQ=60)
  GCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCC                                                                                            <  SRR4302540.4002668/94‑1 (MQ=60)
  GCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCC                                                                                            >  SRR4302540.9211761/1‑94 (MQ=60)
  GCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCC                                                                                            >  SRR4302540.95618/1‑94 (MQ=60)
   CTGGATCCGATCGCTCCCCACGGCGGTTGCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCG                                                                                           <  SRR4302540.316503/94‑1 (MQ=60)
   CTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCG                                                                                           >  SRR4302540.10016377/1‑94 (MQ=60)
   CTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCG                                                                                           <  SRR4302540.316504/94‑1 (MQ=60)
   CTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCG                                                                                           <  SRR4302540.8288388/94‑1 (MQ=60)
   CTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCG                                                                                           <  SRR4302540.1731964/94‑1 (MQ=60)
   CTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCG                                                                                           >  SRR4302540.1256123/1‑94 (MQ=60)
   CTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCG                                                                                           >  SRR4302540.1256122/1‑94 (MQ=60)
    TGGATCAGATCGCTACCCACGGCTGTTTCCGGATGGAAATCAACGTCAAAGGCGTCCTCTATATCGACGATCACCACACCGTCGAAGATGCCGG                                                                                          <  SRR4302540.3067715/94‑1 (MQ=60)
     GGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCACCGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGC                                                                                         >  SRR4302540.4113631/1‑94 (MQ=60)
     GGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGC                                                                                         >  SRR4302540.4113632/1‑94 (MQ=60)
      GATCAGATCGCTACCCACGGCGGTTTCCGCATGGCAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATACCGGCC                                                                                        >  SRR4302540.7692106/1‑94 (MQ=60)
      GATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCC                                                                                        >  SRR4302540.4819705/1‑94 (MQ=60)
      GATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCC                                                                                        <  SRR4302540.3112511/94‑1 (MQ=60)
       ATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCT                                                                                       <  SRR4302540.9367176/94‑1 (MQ=60)
       ATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCT                                                                                       <  SRR4302540.4036088/94‑1 (MQ=60)
       ATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCT                                                                                       >  SRR4302540.6679349/1‑94 (MQ=60)
       ATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCT                                                                                       >  SRR4302540.6679350/1‑94 (MQ=60)
       ATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCT                                                                                       >  SRR4302540.7849903/1‑94 (MQ=60)
       ATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCT                                                                                       >  SRR4302540.7849904/1‑94 (MQ=60)
        TCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTG                                                                                      <  SRR4302540.3075733/94‑1 (MQ=60)
         CAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGG                                                                                     <  SRR4302540.3106515/94‑1 (MQ=60)
         CAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGG                                                                                     >  SRR4302540.7976081/1‑94 (MQ=60)
          AGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGC                                                                                    >  SRR4302540.7947262/1‑94 (MQ=60)
            ATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGC                                                                                  >  SRR4302540.5061761/1‑94 (MQ=60)
            ATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGC                                                                                  >  SRR4302540.8831219/1‑94 (MQ=60)
             TCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCT                                                                                 >  SRR4302540.1207721/1‑94 (MQ=60)
               GCTACCCACGGCGGTTTCCGCATGGACATCAACGTCAAAGGCGACCTCTATATCGTCGATCACCACACCGTCGTAGATGCCGGCCTGGCGCTCG                                                                               >  SRR4302540.9260900/1‑94 (MQ=60)
                CTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGG                                                                              <  SRR4302540.174493/94‑1 (MQ=60)
                CTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGG                                                                              >  SRR4302540.81344/1‑94 (MQ=60)
                CTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGCCCTGGCGCTGGG                                                                              >  SRR4302540.254011/1‑94 (MQ=60)
                  CCCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCG                                                                            <  SRR4302540.4107914/94‑1 (MQ=60)
                    CCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAA                                                                          <  SRR4302540.3491998/94‑1 (MQ=60)
                      ACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGC                                                                        <  SRR4302540.9920811/94‑1 (MQ=60)
                      ACGGCGGTTTCCGCATGGAAATAAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGC                                                                        <  SRR4302540.5482043/94‑1 (MQ=60)
                           GGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAA                                                                   >  SRR4302540.8633730/1‑94 (MQ=60)
                           GGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAA                                                                   >  SRR4302540.445674/1‑94 (MQ=60)
                            GTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAA                                                                  >  SRR4302540.7288602/1‑94 (MQ=60)
                            GTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAA                                                                  >  SRR4302540.1128482/1‑94 (MQ=60)
                             TTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAA                                                                 >  SRR4302540.9761547/1‑94 (MQ=60)
                             TTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAA                                                                 <  SRR4302540.5444062/94‑1 (MQ=60)
                              TTCCGCATGGAAATCAACGTCAAAGGCGGCCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCCGGGCGAACCGCTAACAA                                                                >  SRR4302540.9580836/1‑94 (MQ=60)
                                CCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATC                                                              <  SRR4302540.6852159/94‑1 (MQ=60)
                                 CGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGGGAAGCGCTAAAAATCC                                                             >  SRR4302540.9142565/1‑94 (MQ=60)
                                 CGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCG                                                             >  SRR4302540.9142564/1‑94 (MQ=60)
                                  GCATGGAAATCAACGTCAAAGGCGACCTCTATAGCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGC                                                            <  SRR4302540.9821674/94‑1 (MQ=60)
                                    CTGGAAAACAACGTCAAAGGCGACCTCTATGACGACGCTCACCACACCGTGGAAGATGCCGGCCGGGTGCTGGGCGAAGCGCTAAAAATCGCCC                                                          <  SRR4302540.6975368/94‑1 (MQ=60)
                                    ATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCC                                                          >  SRR4302540.3160095/1‑94 (MQ=60)
                                    ATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCC                                                          >  SRR4302540.2154388/1‑94 (MQ=60)
                                     TGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCT                                                         >  SRR4302540.9334778/1‑94 (MQ=60)
                                      GGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTC                                                        >  SRR4302540.540856/1‑94 (MQ=60)
                                       GAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCG                                                       <  SRR4302540.2720654/94‑1 (MQ=60)
                                        AAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGG                                                      <  SRR4302540.3980454/94‑1 (MQ=60)
                                         AATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGA                                                     >  SRR4302540.9962174/1‑94 (MQ=60)
                                          ATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAG                                                    <  SRR4302540.1844307/94‑1 (MQ=60)
                                          ATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAG                                                    <  SRR4302540.2956484/94‑1 (MQ=60)
                                            CAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGAC                                                  <  SRR4302540.10041424/94‑1 (MQ=60)
                                             AACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGAGAAGCGCTAAAAATCGCCCTCGGAGACA                                                 >  SRR4302540.6700618/1‑94 (MQ=60)
                                              ACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGAGAAGCGCTAAAAATCGCCCTCGGAGACAA                                                >  SRR4302540.1237587/1‑94 (MQ=60)
                                                GTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGACGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAAC                                              >  SRR4302540.140467/1‑94 (MQ=60)
                                                GTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAAC                                              <  SRR4302540.5624482/94‑1 (MQ=60)
                                                GTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAAC                                              >  SRR4302540.140466/1‑94 (MQ=60)
                                                GTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAAC                                              <  SRR4302540.2869866/94‑1 (MQ=60)
                                                  CAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGC                                            >  SRR4302540.5281195/1‑94 (MQ=60)
                                                   AAAGGCGACCTTTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCG                                           <  SRR4302540.513816/94‑1 (MQ=60)
                                                   AAAGGCGACCTCTATATCGACGATCACCACACCGTCCAAGATGCCGGACTCGCGATGGGCGAAACGGCAAAAATCGCCCTCGGgtcacacggcg                                           >  SRR4302540.9715016/1‑83 (MQ=44)
                                                     AGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGT                                         <  SRR4302540.9464090/94‑1 (MQ=60)
                                                     AGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGT                                         >  SRR4302540.1913338/1‑94 (MQ=60)
                                                     AGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGT                                         >  SRR4302540.5354885/1‑94 (MQ=60)
                                                       GCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTAT                                       >  SRR4302540.4562915/1‑94 (MQ=60)
                                                       GCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTAT                                       <  SRR4302540.10140999/94‑1 (MQ=60)
                                                        CGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATT                                      >  SRR4302540.232203/1‑94 (MQ=60)
                                                        CGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATT                                      >  SRR4302540.3387628/1‑94 (MQ=60)
                                                         ccCCTCTATATCGACGATCCCCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTT                                     <  SRR4302540.3770854/92‑1 (MQ=60)
                                                         GACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTT                                     <  SRR4302540.1147916/94‑1 (MQ=60)
                                                         GACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTT                                     <  SRR4302540.2128708/94‑1 (MQ=60)
                                                         GACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTT                                     >  SRR4302540.9313625/1‑94 (MQ=60)
                                                         GACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTT                                     <  SRR4302540.6586331/94‑1 (MQ=60)
                                                         GACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTT                                     >  SRR4302540.5498242/1‑94 (MQ=60)
                                                         CACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTT                                     <  SRR4302540.3770525/94‑1 (MQ=60)
                                                           CCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGC                                   <  SRR4302540.5854806/94‑1 (MQ=60)
                                                           CCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGC                                   >  SRR4302540.173239/1‑94 (MQ=60)
                                                              CTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGC                                >  SRR4302540.6240726/1‑94 (MQ=60)
                                                                ATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTT                              >  SRR4302540.3150696/1‑94 (MQ=60)
                                                                ATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCGT                              >  SRR4302540.7154448/1‑94 (MQ=60)
                                                                AGATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTT                              <  SRR4302540.4317951/94‑1 (MQ=60)
                                                                 TATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTT                             >  SRR4302540.6854/1‑94 (MQ=60)
                                                                  ATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTG                            <  SRR4302540.691714/94‑1 (MQ=60)
                                                                    CGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGT                          >  SRR4302540.6179028/1‑94 (MQ=60)
                                                                    CGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGT                          <  SRR4302540.7108856/94‑1 (MQ=60)
                                                                    CGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGT                          <  SRR4302540.1621338/94‑1 (MQ=60)
                                                                     GACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTT                         <  SRR4302540.7437755/94‑1 (MQ=60)
                                                                     GACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTT                         <  SRR4302540.3809682/94‑1 (MQ=60)
                                                                      ACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTT                        >  SRR4302540.7184893/1‑94 (MQ=60)
                                                                      ACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTT                        <  SRR4302540.7883511/94‑1 (MQ=60)
                                                                        GATCACCACACCGTCGAAGATGCCGGCCTGGTGCTGGGCGAAGCGCTACAAATCGCCCTCGGCGACAAACGCGGCATTTGCCGCTGTGGTTTTG                      >  SRR4302540.4770382/1‑94 (MQ=60)
                                                                        GATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTG                      >  SRR4302540.4770381/1‑94 (MQ=60)
                                                                         ATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGT                     >  SRR4302540.8015072/1‑94 (MQ=60)
                                                                         ATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGT                     <  SRR4302540.2801486/94‑1 (MQ=60)
                                                                         ATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGT                     >  SRR4302540.5934720/1‑94 (MQ=60)
                                                                          TCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTG                    <  SRR4302540.5466294/94‑1 (MQ=60)
                                                                          TCACCACACCGACGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTG                    <  SRR4302540.5466293/94‑1 (MQ=60)
                                                                           CACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGC                   <  SRR4302540.2214574/94‑1 (MQ=60)
                                                                             CCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTA                 <  SRR4302540.7891387/94‑1 (MQ=60)
                                                                              CACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTAC                >  SRR4302540.9493659/1‑94 (MQ=60)
                                                                               ACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGTTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACC               >  SRR4302540.4931744/1‑94 (MQ=60)
                                                                                CACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCG              <  SRR4302540.4356131/94‑1 (MQ=60)
                                                                                CACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCG              <  SRR4302540.2681159/94‑1 (MQ=60)
                                                                                  CCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGTCGCTTTGGTTTTGTGCTACCGAT            <  SRR4302540.3120415/94‑1 (MQ=60)
                                                                                  CCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGAT            <  SRR4302540.7431891/94‑1 (MQ=60)
                                                                                  CCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGAT            <  SRR4302540.7431890/94‑1 (MQ=60)
                                                                                  CCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGAT            <  SRR4302540.9772622/94‑1 (MQ=60)
                                                                                   CGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATG           >  SRR4302540.8132631/1‑94 (MQ=60)
                                                                                   CGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATG           >  SRR4302540.1091647/1‑94 (MQ=60)
                                                                                    GTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGG          <  SRR4302540.8432364/94‑1 (MQ=60)
                                                                                    GTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGG          <  SRR4302540.9269023/94‑1 (MQ=60)
                                                                                      CGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGAC        >  SRR4302540.2247235/1‑94 (MQ=60)
                                                                                       GAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACG       >  SRR4302540.988110/1‑94 (MQ=60)
                                                                                       GAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACG       >  SRR4302540.465608/1‑94 (MQ=60)
                                                                                       GAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACG       >  SRR4302540.1661983/1‑94 (MQ=60)
                                                                                       GAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACG       <  SRR4302540.300400/94‑1 (MQ=60)
                                                                                       GAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACG       <  SRR4302540.543423/94‑1 (MQ=60)
                                                                                         AGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAA     >  SRR4302540.3978842/1‑94 (MQ=60)
                                                                                          GATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAAT    >  SRR4302540.8435553/1‑94 (MQ=60)
                                                                                          GATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAAT    >  SRR4302540.8435551/1‑94 (MQ=60)
                                                                                          GATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAAT    <  SRR4302540.117312/94‑1 (MQ=60)
                                                                                          GATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAAT    <  SRR4302540.10120124/94‑1 (MQ=60)
                                                                                           ATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAATG   <  SRR4302540.7168732/94‑1 (MQ=60)
                                                                                           ATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAATG   <  SRR4302540.3267753/94‑1 (MQ=60)
                                                                                           ATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAATG   <  SRR4302540.5438818/94‑1 (MQ=60)
                                                                                            TGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAATGC  >  SRR4302540.1470438/1‑94 (MQ=60)
                                                                                            TGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAATGC  >  SRR4302540.886212/1‑94 (MQ=60)
                                                                                             |                                                                                            
ATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATACCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAATGC  >  NC_000913/2092099‑2092284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑