Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,133,804	T→C	H106R (CAC→CGC)	wzb ←	putative protein‑tyrosine‑phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,133,804	0	T	C	99.6%	781.7 / ‑4.9	246	H106R (CAC→CGC)	wzb	putative protein‑tyrosine‑phosphatase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (109/136); total (110/136)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.41e-01

Only 100 of 260 total aligned reads displayed.

GGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCG  >  NC_000913/2133715‑2133893

gggCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGGTATCCCAGCGAc                                                                                        >  1:1043577/1‑93 (MQ=255)

 ggCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGAcc                                                                                       >  1:4589798/1‑93 (MQ=255)

 ggCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGAcc                                                                                       >  1:3455996/1‑93 (MQ=255)

  gCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCa                                                                                      >  2:1217659/1‑93 (MQ=255)

   cAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCaa                                                                                     >  1:4153306/1‑93 (MQ=255)

    aGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCcaa                                                                                    >  1:7076110/1‑93 (MQ=255)

    aGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCaaa                                                                                    <  2:2639727/93‑1 (MQ=255)

     gACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAAc                                                                                   >  1:6989422/1‑93 (MQ=255)

     gACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAAc                                                                                   <  2:7961460/93‑1 (MQ=255)

     gACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCAGGGATTTCACATTCGTTATCCCAGCGACCAAAc                                                                                   <  1:678651/93‑1 (MQ=255)

       ccGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACac                                                                                 >  2:9766539/1‑92 (MQ=255)

       ccGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAg                                                                                 >  1:4957254/1‑93 (MQ=255)

        cGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGc                                                                                >  2:5993631/1‑93 (MQ=255)

        cGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGc                                                                                <  1:3634809/93‑1 (MQ=255)

         gTTCAAGTGATGTGTACGCCGCTGCAAACGTTTCCCGGCTTATACGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCa                                                                               <  2:8979890/93‑1 (MQ=255)

          ttCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCAt                                                                              <  1:1880625/93‑1 (MQ=255)

          ttCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCAt                                                                              <  2:7657741/93‑1 (MQ=255)

          ttCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTCATCCCAGCGACCAAACAGCAt                                                                              >  2:2801680/1‑93 (MQ=255)

           tCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATc                                                                             >  2:3638675/1‑93 (MQ=255)

            cAAGAAATGTGTCCACCGCGGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCTTTTTCCCAGCGACC‑AACAGCATCAc                                                                           <  2:4542118/93‑1 (MQ=255)

              aGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACt                                                                          >  2:6575947/1‑93 (MQ=255)

                tAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACttt                                                                        >  1:8269876/1‑93 (MQ=255)

                tAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACttt                                                                        <  2:4370664/93‑1 (MQ=255)

                  aTGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGc                                                                      <  2:354275/93‑1 (MQ=255)

                   tgtgTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGcc                                                                     >  2:254052/1‑93 (MQ=255)

                    gtgtACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCcg                                                                    >  2:1865675/1‑93 (MQ=255)

                    gtgtACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGACTTGACATTCGCTATCCCAGCGACCAAACAGCATAACTTTGctg                                                                    >  2:4960544/1‑91 (MQ=255)

                        acacCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCa                                                                <  2:6739492/93‑1 (MQ=255)

                        acacCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTAGCCGCGCa                                                                <  2:324888/93‑1 (MQ=255)

                           ccGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAtct                                                             >  2:5252720/1‑93 (MQ=255)

                           ccGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAtct                                                             <  1:1237050/93‑1 (MQ=255)

                           ccGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGGTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAtct                                                             <  1:3282870/93‑1 (MQ=255)

                            cGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAtctc                                                            <  1:2606356/93‑1 (MQ=255)

                            cGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAtctc                                                            <  2:8080275/93‑1 (MQ=255)

                              cTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCg                                                          <  2:1439219/93‑1 (MQ=255)

                              cTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCg                                                          >  1:9079793/1‑93 (MQ=255)

                              cTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCg                                                          >  1:9993467/1‑93 (MQ=255)

                              cTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCg                                                          <  1:5957647/93‑1 (MQ=255)

                              cTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCg                                                          <  1:6616361/93‑1 (MQ=255)

                               tGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCgg                                                         <  2:7406806/93‑1 (MQ=255)

                               tGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCgg                                                         <  1:7739341/93‑1 (MQ=255)

                               tGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCgg                                                         <  1:6262028/93‑1 (MQ=255)

                               tGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCgg                                                         <  1:8820196/93‑1 (MQ=255)

                                 cAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTg                                                       >  1:3234965/1‑93 (MQ=255)

                                 cAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTg                                                       >  1:3146159/1‑93 (MQ=255)

                                 cAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTg                                                       >  2:4037002/1‑93 (MQ=255)

                                 cAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTg                                                       >  2:789076/1‑93 (MQ=255)

                                   aaCGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGcc                                                     >  2:3332373/1‑93 (MQ=255)

                                       tttCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAtct                                                 >  1:8701319/1‑93 (MQ=255)

                                        ttCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAtctc                                                <  2:8371290/93‑1 (MQ=255)

                                           ccGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCa                                             <  1:2482226/93‑1 (MQ=255)

                                           ccGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCa                                             >  2:4822262/1‑93 (MQ=255)

                                             ggCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATa                                           >  1:4435520/1‑93 (MQ=255)

                                              gCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATaa                                          >  2:2451539/1‑93 (MQ=255)

                                                ttttGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGc                                        >  2:7258696/1‑93 (MQ=255)

                                                ttttGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGc                                        >  1:9789997/1‑93 (MQ=255)

                                                   tGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGtt                                     <  1:4801752/93‑1 (MQ=255)

                                                   tGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGtt                                     <  1:9016951/93‑1 (MQ=255)

                                                    gCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTc                                    >  2:9602862/1‑93 (MQ=255)

                                                    gCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTc                                    <  1:9705831/93‑1 (MQ=255)

                                                     cGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCCGTGCCATCTCGCATAAGCGTTCg                                   >  2:5524005/1‑93 (MQ=255)

                                                      gATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                  >  1:1962445/1‑93 (MQ=255)

                                                      gATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                  <  2:6443069/93‑1 (MQ=255)

                                                       aTACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGat                                 >  1:8305097/1‑93 (MQ=255)

                                                        tACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGata                                <  2:2764840/93‑1 (MQ=255)

                                                        tACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGata                                <  1:4043704/93‑1 (MQ=255)

                                                        tACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGata                                <  1:5740487/93‑1 (MQ=255)

                                                        tACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGata                                <  1:7099856/93‑1 (MQ=255)

                                                        tACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGata                                <  2:8809774/93‑1 (MQ=255)

                                                        tACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGata                                <  2:1785082/93‑1 (MQ=255)

                                                         aCGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGatat                               >  2:4160702/1‑93 (MQ=255)

                                                          cGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATg                              <  1:4743981/93‑1 (MQ=255)

                                                           ggATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATgg                             <  1:4830580/93‑1 (MQ=255)

                                                            gATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGgc                            >  2:3521649/1‑93 (MQ=255)

                                                            gATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGgc                            <  1:7943963/93‑1 (MQ=255)

                                                             aTCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGgcg                           >  1:6305420/1‑93 (MQ=255)

                                                             aTCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGgcg                           >  2:5070659/1‑93 (MQ=255)

                                                             aTCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGgcg                           <  1:3957801/93‑1 (MQ=255)

                                                              tCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGgcgc                          <  1:5464646/93‑1 (MQ=255)

                                                               cGGTGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCt                         <  2:3069387/93‑1 (MQ=255)

                                                                ggggATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCtt                        >  1:2981216/1‑93 (MQ=255)

                                                                 gggATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCttt                       <  2:6400636/93‑1 (MQ=255)

                                                                 gggATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGCCGCttt                       >  2:8572987/1‑93 (MQ=255)

                                                                    aTTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTcc                    >  2:913198/1‑93 (MQ=255)

                                                                     tttCACATTCGTTATCCCAGCGACCAAACAGCATCCCTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGAGTTCGATATGGCGCTTTTCCa                   <  1:1808467/93‑1 (MQ=255)

                                                                     tttCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCa                   <  1:6289617/93‑1 (MQ=255)

                                                                     tttCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCa                   <  1:9461945/93‑1 (MQ=255)

                                                                      ttCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCAt                  >  2:1067138/1‑93 (MQ=255)

                                                                       tCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATg                 <  1:9158458/93‑1 (MQ=255)

                                                                           aTTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCa             >  2:3917340/1‑93 (MQ=255)

                                                                              cGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCaaaa          >  1:72638/1‑93 (MQ=255)

                                                                              cGTTATCCCAGCGACCAAACAGAATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCaaaa          <  1:7911563/93‑1 (MQ=255)

                                                                                ttATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATc        >  2:20661/1‑93 (MQ=255)

                                                                                 tATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCa       >  1:1532363/1‑93 (MQ=255)

                                                                                 tATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCAGGGTCAAAATCa       <  1:284729/93‑1 (MQ=255)

                                                                                   tCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGGGCCATCTCGCATAAGCGTTCGATATGGCGCTTCTCCATGGTCAAAATCAgg     >  1:5353666/1‑93 (MQ=255)

                                                                                    cccAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGt    <  1:579708/93‑1 (MQ=255)

                                                                                    cccAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGt    <  2:4657849/93‑1 (MQ=255)

                                                                                    cccAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGt    >  1:1425964/1‑93 (MQ=255)

                                                                                      cAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCg  <  2:563796/93‑1 (MQ=255)

GGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCG  >  NC_000913/2133715‑2133893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGT  >  NC_000913/2133711‑2133897

TGGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGC                                                                                               <  SRR4302540.5060072/94‑1 (MQ=60)

  GCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGA                                                                                             <  SRR4302540.992671/94‑1 (MQ=60)

   CGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGGTATCCCAGCGAC                                                                                            >  SRR4302540.1054183/1‑94 (MQ=60)

    GGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACC                                                                                           >  SRR4302540.3493607/1‑94 (MQ=60)

    GGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACC                                                                                           >  SRR4302540.4639208/1‑94 (MQ=60)

      GCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAA                                                                                         >  SRR4302540.4198294/1‑94 (MQ=60)

       CAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCCAA                                                                                        >  SRR4302540.7154850/1‑94 (MQ=60)

       CAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAA                                                                                        >  SRR4302540.9652365/1‑94 (MQ=60)

        AGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTTCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAAC                                                                                       >  SRR4302540.7286237/1‑94 (MQ=60)

        AGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAAC                                                                                       >  SRR4302540.3071375/1‑94 (MQ=60)

        AGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAAC                                                                                       >  SRR4302540.7067012/1‑94 (MQ=60)

         GACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACA                                                                                      >  SRR4302540.2682917/1‑94 (MQ=60)

         GACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCAGGGATTTCACATTCGTTATCCCAGCGACCAAACA                                                                                      <  SRR4302540.685918/94‑1 (MQ=60)

          ACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAG                                                                                     <  SRR4302540.1425726/94‑1 (MQ=60)

          ACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAG                                                                                     >  SRR4302540.277801/1‑94 (MQ=60)

          ACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAG                                                                                     >  SRR4302540.5010828/1‑94 (MQ=60)

          ACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAG                                                                                     <  SRR4302540.2091841/94‑1 (MQ=60)

           CCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGC                                                                                    <  SRR4302540.6097877/94‑1 (MQ=60)

           CCGCTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGC                                                                                    <  SRR4302540.9188371/94‑1 (MQ=60)

            CGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCA                                                                                   <  SRR4302540.3674598/94‑1 (MQ=60)

             GTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCAT                                                                                  >  SRR4302540.4743149/1‑94 (MQ=60)

             GTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCAT                                                                                  <  SRR4302540.5140683/94‑1 (MQ=60)

             GTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCAT                                                                                  <  SRR4302540.6967976/94‑1 (MQ=60)

              TTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGGTTTCACATTCGTTATCCCAGCGACCAAACAGCATC                                                                                 <  SRR4302540.7556093/94‑1 (MQ=60)

              TTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATC                                                                                 >  SRR4302540.7036678/1‑94 (MQ=60)

              TTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATC                                                                                 <  SRR4302540.1901220/94‑1 (MQ=60)

              TTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATC                                                                                 >  SRR4302540.6012991/1‑94 (MQ=60)

                CAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCTATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCAC                                                                               <  SRR4302540.8218255/94‑1 (MQ=60)

                CAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCAC                                                                               <  SRR4302540.635687/94‑1 (MQ=60)

                CAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCAC                                                                               >  SRR4302540.8107947/1‑94 (MQ=60)

                 AAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACT                                                                              <  SRR4302540.9374243/94‑1 (MQ=60)

                  AGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTT                                                                             >  SRR4302540.8025595/1‑94 (MQ=60)

                   GTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGGTTTCACATTCGGTATCCCCGCGACCCAACAGCATCACTTT                                                                            >  SRR4302540.337197/1‑94 (MQ=60)

                   GTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTT                                                                            <  SRR4302540.4041590/94‑1 (MQ=60)

                   GTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTT                                                                            >  SRR4302540.8365965/1‑94 (MQ=60)

                   GTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTT                                                                            >  SRR4302540.8425092/1‑94 (MQ=60)

                    TAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTG                                                                           <  SRR4302540.1600026/94‑1 (MQ=60)

                     AATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGC                                                                          >  SRR4302540.1005303/1‑94 (MQ=60)

                         TGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATATGGATCGTGTGTTTCACATTCGTTGTCCCAGCGACAAAACAGCATAACTTGGCCGCG                                                                      >  SRR4302540.639872/1‑94 (MQ=53)

                          GTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGC                                                                     <  SRR4302540.9010835/94‑1 (MQ=60)

                             CACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATC                                                                  >  SRR4302540.2985942/1‑94 (MQ=60)

                               CCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTC                                                                <  SRR4302540.1249679/94‑1 (MQ=60)

                               CCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGGTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTC                                                                <  SRR4302540.3318752/94‑1 (MQ=60)

                                cccgggAAACGTTTCCCCGCTGTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCC                                                               <  SRR4302540.7716213/88‑1 (MQ=60)

                                CGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCC                                                               >  SRR4302540.1845498/1‑94 (MQ=60)

                                CGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCC                                                               <  SRR4302540.6064160/94‑1 (MQ=60)

                                CGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCC                                                               <  SRR4302540.2634488/94‑1 (MQ=60)

                                 GCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCG                                                              >  SRR4302540.9188868/1‑94 (MQ=60)

                                 GCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCG                                                              <  SRR4302540.6715922/94‑1 (MQ=60)

                                 GCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCG                                                              <  SRR4302540.7171905/94‑1 (MQ=60)

                                 GCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCG                                                              >  SRR4302540.10109931/1‑94 (MQ=60)

                                  CTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGG                                                             <  SRR4302540.6689638/94‑1 (MQ=60)

                                  CTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGG                                                             <  SRR4302540.6023540/94‑1 (MQ=60)

                                   TGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCCTCTCCGGG                                                            >  SRR4302540.2583614/1‑94 (MQ=60)

                                   TGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGT                                                            <  SRR4302540.8922180/94‑1 (MQ=60)

                                   TGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGT                                                            <  SRR4302540.6331695/94‑1 (MQ=60)

                                   TGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGT                                                            <  SRR4302540.7829697/94‑1 (MQ=60)

                                    GCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCGCTTTGCCGCGCATCTCCGGTG                                                           >  SRR4302540.5995925/1‑94 (MQ=60)

                                    GCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTG                                                           >  SRR4302540.3270145/1‑94 (MQ=60)

                                    GCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTG                                                           >  SRR4302540.3180436/1‑94 (MQ=60)

                                    GCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTG                                                           <  SRR4302540.88982/94‑1 (MQ=60)

                                     CAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGC                                                          >  SRR4302540.3526731/1‑94 (MQ=60)

                                      AAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCC                                                         <  SRR4302540.3568894/94‑1 (MQ=60)

                                      AAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCC                                                         >  SRR4302540.185984/1‑94 (MQ=60)

                                      AAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCC                                                         >  SRR4302540.401134/1‑94 (MQ=60)

                                      AAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCC                                                         >  SRR4302540.1490074/1‑94 (MQ=60)

                                        ACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAT                                                       <  SRR4302540.579825/94‑1 (MQ=60)

                                         CGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATC                                                      <  SRR4302540.5496772/94‑1 (MQ=60)

                                          GTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCT                                                     >  SRR4302540.9689461/1‑94 (MQ=60)

                                          GTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCT                                                     >  SRR4302540.8802321/1‑94 (MQ=60)

                                           TTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTC                                                    <  SRR4302540.8495409/94‑1 (MQ=60)

                                           TTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTC                                                    <  SRR4302540.4394508/94‑1 (MQ=60)

                                               CCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCAT                                                <  SRR4302540.2508968/94‑1 (MQ=60)

                                                CGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATA                                               >  SRR4302540.4483579/1‑94 (MQ=60)

                                                 GGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAA                                              >  SRR4302540.9876636/1‑94 (MQ=60)

                                                 GGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAA                                              <  SRR4302540.4617053/94‑1 (MQ=60)

                                                 GGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAA                                              >  SRR4302540.3843701/1‑94 (MQ=60)

                                                 GGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAA                                              >  SRR4302540.7171002/1‑94 (MQ=60)

                                                   CTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGC                                            >  SRR4302540.5126755/1‑94 (MQ=60)

                                                   CTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGC                                            >  SRR4302540.9904686/1‑94 (MQ=60)

                                                   CTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGC                                            >  SRR4302540.4353738/1‑94 (MQ=60)

                                                    TTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGACGCGCATCTCCGGTGACATCTCGCATAAGCG                                           >  SRR4302540.795941/1‑94 (MQ=60)

                                                     TTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGT                                          >  SRR4302540.7467468/1‑94 (MQ=60)

                                                      TTGCGATACGGATCGGTGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTT                                         <  SRR4302540.1975182/94‑1 (MQ=60)

                                                       TGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTC                                        >  SRR4302540.993643/1‑94 (MQ=60)

                                                       TGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTC                                        <  SRR4302540.9125148/94‑1 (MQ=60)

                                                       TGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTC                                        <  SRR4302540.1010008/94‑1 (MQ=60)

                                                       TGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTC                                        <  SRR4302540.4853139/94‑1 (MQ=60)

                                                       TGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTC                                        <  SRR4302540.4853141/94‑1 (MQ=60)

                                                        GCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCG                                       <  SRR4302540.9819908/94‑1 (MQ=60)

                                                         CGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGA                                      >  SRR4302540.1983763/1‑94 (MQ=60)

                                                          GATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGAT                                     >  SRR4302540.8401534/1‑94 (MQ=60)

                                                           ATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATA                                    <  SRR4302540.1366002/94‑1 (MQ=60)

                                                           ATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATA                                    >  SRR4302540.3276585/1‑94 (MQ=60)

                                                            TACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATAT                                   <  SRR4302540.4569036/94‑1 (MQ=60)

                                                            TACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATAT                                   <  SRR4302540.5803762/94‑1 (MQ=60)

                                                            TACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATAT                                   <  SRR4302540.7949200/94‑1 (MQ=60)

                                                            TACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATAT                                   <  SRR4302540.7949207/94‑1 (MQ=60)

                                                            TACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATAT                                   <  SRR4302540.4087299/94‑1 (MQ=60)

                                                            TACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATAT                                   <  SRR4302540.7178858/94‑1 (MQ=60)

                                                             ACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCTATCTCGCATAAGCGTTCGATATG                                  >  SRR4302540.9549075/1‑94 (MQ=60)

                                                             ACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATG                                  <  SRR4302540.4850176/94‑1 (MQ=60)

                                                              CGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGG                                 <  SRR4302540.4794605/94‑1 (MQ=60)

                                                               GGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGC                                <  SRR4302540.4882323/94‑1 (MQ=60)

                                                               GGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGC                                <  SRR4302540.4882322/94‑1 (MQ=60)

                                                                GATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCG                               >  SRR4302540.6375636/1‑94 (MQ=60)

                                                                GATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCG                               <  SRR4302540.8036557/94‑1 (MQ=60)

                                                                 ATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGC                              <  SRR4302540.710494/94‑1 (MQ=60)

                                                                 ATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGC                              <  SRR4302540.4000497/94‑1 (MQ=60)

                                                                 ATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGC                              >  SRR4302540.5660651/1‑94 (MQ=60)

                                                                  TCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCT                             <  SRR4302540.5524797/94‑1 (MQ=60)

                                                                  TCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCT                             <  SRR4302540.3330414/94‑1 (MQ=60)

                                                                   CGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTT                            >  SRR4302540.3014133/1‑94 (MQ=60)

                                                                    GGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTT                           <  SRR4302540.1738933/94‑1 (MQ=60)

                                                                       GATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCC                        >  SRR4302540.484129/1‑94 (MQ=60)

                                                                        ATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCA                       >  SRR4302540.4895588/1‑94 (MQ=60)

                                                                         TTTCACATTCGTTATCCCAGCGACCAAACAGCATCCCTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGAGTTCGATATGGCGCTTTTCCAT                      <  SRR4302540.1828171/94‑1 (MQ=60)

                                                                         TTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCAT                      <  SRR4302540.6359627/94‑1 (MQ=60)

                                                                         TTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCAT                      <  SRR4302540.6359626/94‑1 (MQ=60)

                                                                         TTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCAT                      <  SRR4302540.6359628/94‑1 (MQ=60)

                                                                         TTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCAT                      <  SRR4302540.9574211/94‑1 (MQ=60)

                                                                          TTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATG                     <  SRR4302540.583336/94‑1 (MQ=60)

                                                                           TCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGG                    <  SRR4302540.9268393/94‑1 (MQ=60)

                                                                           TCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGG                    <  SRR4302540.9143746/94‑1 (MQ=60)

                                                                            CACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGT                   >  SRR4302540.9254508/1‑94 (MQ=60)

                                                                            CACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGT                   >  SRR4302540.4192664/1‑94 (MQ=60)

                                                                             ACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTC                  <  SRR4302540.1200767/94‑1 (MQ=60)

                                                                                TTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAA               <  SRR4302540.8389364/94‑1 (MQ=60)

                                                                                 TCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAA              >  SRR4302540.73385/1‑94 (MQ=60)

                                                                                  CGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAAT             <  SRR4302540.8903770/94‑1 (MQ=60)

                                                                                  CGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAAT             >  SRR4302540.3218823/1‑94 (MQ=60)

                                                                                  CGTTATCCCAGCGACCAAACAGAATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAAT             <  SRR4302540.8003810/94‑1 (MQ=60)

                                                                                   GTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATC            >  SRR4302540.1993311/1‑94 (MQ=60)

                                                                                    TTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCA           >  SRR4302540.1549330/1‑94 (MQ=60)

                                                                                     TATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCAGGGTCAAAATCAG          <  SRR4302540.287532/94‑1 (MQ=60)

                                                                                      ATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGGGCCATCTCGCATAAGCGTTCGATATGGCGCTTCTCCATGGTCAAAATCAGG         >  SRR4302540.5412446/1‑94 (MQ=60)

                                                                                       TCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGT        >  SRR4302540.1441400/1‑94 (MQ=60)

                                                                                        CCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTC       <  SRR4302540.291363/94‑1 (MQ=60)

                                                                                        CCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTC       <  SRR4302540.585980/94‑1 (MQ=60)

                                                                                         CCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTTCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCG      <  SRR4302540.6826055/94‑1 (MQ=60)

                                                                                         CCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCG      <  SRR4302540.3237392/94‑1 (MQ=60)

                                                                                           AGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTA    >  SRR4302540.4631083/1‑94 (MQ=60)

                                                                                           AGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTA    <  SRR4302540.5248001/94‑1 (MQ=60)

                                                                                            GCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAG   <  SRR4302540.7483622/94‑1 (MQ=60)

                                                                                            GCGACAAAACAGCATCACTTTACCGCGCAGCTCCGTTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAG   <  SRR4302540.1826116/94‑1 (MQ=60)

                                                                                             CGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGT  <  SRR4302540.3628471/94‑1 (MQ=60)

                                                                                             CGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGT  >  SRR4302540.9606193/1‑94 (MQ=60)

                                                                                             CGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGT  <  SRR4302540.1540814/94‑1 (MQ=60)

TGGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGT  >  NC_000913/2133711‑2133897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`