Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,133,804 T→C H106R (CAC→CGC)  wzb ← putative protein‑tyrosine‑phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,133,8040TC99.6% 813.9 / NA 255H106R (CAC→CGC) wzbputative protein‑tyrosine‑phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (125/129);  minor base G (1/0);  total (126/129)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

GGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCG  >  NC_000913/2133715‑2133893
                                                                                         |                                                                                         
gggCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGAc                                                                                        >  1:616352/1‑93 (MQ=255)
 ggCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCTAGCGAcc                                                                                       >  1:1456258/1‑93 (MQ=255)
    aGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGAGTTCACATTCGTTATCCCAGCGACCcaa                                                                                    >  1:160310/1‑93 (MQ=255)
     gACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAAc                                                                                   <  2:746583/93‑1 (MQ=255)
     gACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAAc                                                                                   >  2:3186429/1‑93 (MQ=255)
       ccGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAg                                                                                 <  1:5731208/93‑1 (MQ=255)
        cGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGc                                                                                >  1:5376182/1‑93 (MQ=255)
         gTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCa                                                                               >  1:3369139/1‑93 (MQ=255)
         gTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCa                                                                               <  2:550434/93‑1 (MQ=255)
           tCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATc                                                                             >  2:1108275/1‑93 (MQ=255)
           tCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATc                                                                             <  2:2664551/93‑1 (MQ=255)
           tCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATc                                                                             <  1:2209235/93‑1 (MQ=255)
            cAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCa                                                                            >  1:4815039/1‑93 (MQ=255)
              aGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACt                                                                          >  2:1072196/1‑93 (MQ=255)
               gTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACtt                                                                         <  2:4680896/93‑1 (MQ=255)
               gTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACtt                                                                         <  1:1631733/93‑1 (MQ=255)
                tAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACttt                                                                        <  1:112258/93‑1 (MQ=255)
                    gtgtACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCcg                                                                    >  2:2781846/1‑93 (MQ=255)
                      gtACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCcgcg                                                                  <  2:3754197/93‑1 (MQ=255)
                        acacCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCa                                                                >  1:271682/1‑93 (MQ=255)
                        acacCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCa                                                                >  1:6207120/1‑93 (MQ=255)
                        acacCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGACGCGCa                                                                >  1:3859319/1‑93 (MQ=255)
                          acCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAtc                                                              <  2:4995399/93‑1 (MQ=255)
                          acCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAtc                                                              <  2:1663377/93‑1 (MQ=255)
                            cGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAtctc                                                            <  2:3876356/93‑1 (MQ=255)
                            cGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAtctc                                                            <  2:441691/93‑1 (MQ=255)
                            cGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAtctc                                                            <  2:5109531/93‑1 (MQ=255)
                             gCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTcc                                                           <  1:4571214/93‑1 (MQ=255)
                              cTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCg                                                          <  1:1347916/93‑1 (MQ=255)
                               tGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCgg                                                         <  2:1219105/93‑1 (MQ=255)
                               tGCAAACGTTTCCCGGCTCTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCgg                                                         <  2:5349314/93‑1 (MQ=255)
                                gCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGt                                                        <  2:5681904/93‑1 (MQ=255)
                                gCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGt                                                        <  1:821404/93‑1 (MQ=255)
                                gCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGt                                                        <  2:4623914/93‑1 (MQ=255)
                                 cAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTg                                                       >  1:474072/1‑93 (MQ=255)
                                   aaCGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGGGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTgac                                                     >  2:2861785/1‑91 (MQ=255)
                                   aaCGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGcc                                                     >  1:4426701/1‑93 (MQ=255)
                                   aaCGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGcc                                                     <  1:338064/93‑1 (MQ=255)
                                   aaCGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGcc                                                     >  1:3363529/1‑93 (MQ=255)
                                   aaCGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGcc                                                     >  2:4269515/1‑93 (MQ=255)
                                   aaCGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGcc                                                     >  1:2666033/1‑93 (MQ=255)
                                    aCGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAAc                                                                                   <  2:917793/62‑1 (MQ=255)
                                    aCGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAAc                                                                                   >  1:917793/1‑62 (MQ=255)
                                    aCGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCa                                                    >  1:1846428/1‑93 (MQ=255)
                                     cGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAt                                                   <  2:1486699/93‑1 (MQ=255)
                                     cGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAt                                                   >  1:4475830/1‑93 (MQ=255)
                                      gTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAtc                                                  >  1:2429292/1‑93 (MQ=255)
                                       tttCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAtct                                                 <  1:5176035/93‑1 (MQ=255)
                                        ttCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAtctc                                                <  1:2974582/93‑1 (MQ=255)
                                           ccGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCa                                             >  1:105706/1‑93 (MQ=255)
                                           ccGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCa                                             >  1:4280918/1‑93 (MQ=255)
                                             ggCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATa                                           <  2:5896006/93‑1 (MQ=255)
                                             ggCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATa                                           >  2:3983306/1‑93 (MQ=255)
                                              gCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTTCCGCGCATCTCCGGTGCCATCTCGCATaa                                          >  2:2572480/1‑93 (MQ=255)
                                              gCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATaa                                          <  1:3966539/93‑1 (MQ=255)
                                                ttttGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAgt                                        >  1:3045912/1‑92 (MQ=255)
                                                ttttGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGc                                        >  2:681081/1‑93 (MQ=255)
                                                ttttGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGc                                        >  1:5988347/1‑93 (MQ=255)
                                                ttttGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGc                                        >  1:3660613/1‑93 (MQ=255)
                                                ttttGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGc                                        <  2:2516402/93‑1 (MQ=255)
                                                 tttGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCg                                       <  2:5317982/93‑1 (MQ=255)
                                                  ttGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGt                                      <  2:4107929/93‑1 (MQ=255)
                                                     cGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCg                                   >  2:2237197/1‑93 (MQ=255)
                                                     cGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCg                                   <  1:4523842/93‑1 (MQ=255)
                                                      gATACGGATCGGGTATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                  <  2:3449849/93‑1 (MQ=255)
                                                       aTACGGATCGGTGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCTCTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGat                                 <  1:2142575/93‑1 (MQ=255)
                                                       aTACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGat                                 >  1:3274618/1‑93 (MQ=255)
                                                        tACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGata                                >  2:1874844/1‑93 (MQ=255)
                                                        tACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGata                                <  2:1302046/93‑1 (MQ=255)
                                                        tACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGata                                >  2:5758098/1‑93 (MQ=255)
                                                        tACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGata                                <  1:5037742/93‑1 (MQ=255)
                                                         aCGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGatat                               <  1:5924784/93‑1 (MQ=255)
                                                          cGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATg                              <  1:411213/93‑1 (MQ=255)
                                                           ggATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATgg                             >  2:1955529/1‑93 (MQ=255)
                                                            gATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGgc                            <  2:5379626/93‑1 (MQ=255)
                                                             aTCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGgcg                           >  2:2439144/1‑93 (MQ=255)
                                                              tCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGgcgc                          <  2:3497532/93‑1 (MQ=255)
                                                                 gggATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCttt                       <  1:190147/93‑1 (MQ=255)
                                                                 gggATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCttt                       >  1:3496183/1‑93 (MQ=255)
                                                                  ggATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCtttt                      >  2:3048171/1‑93 (MQ=255)
                                                                   gATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTc                     >  1:5244931/1‑93 (MQ=255)
                                                                   gATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTc                     <  2:3293148/93‑1 (MQ=255)
                                                                    aTTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTcc                    <  2:1170653/93‑1 (MQ=255)
                                                                     tttCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATTTCGCATAAGCGTTCGATATGGCGCTTTTCCa                   >  1:1812965/1‑93 (MQ=255)
                                                                      ttCACCTTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCAt                  <  2:4822352/93‑1 (MQ=255)
                                                                      ttCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCAt                  >  2:1606876/1‑93 (MQ=255)
                                                                        cacaTTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATgg                >  2:4305586/1‑93 (MQ=255)
                                                                        cacaTTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATgg                >  1:4680136/1‑93 (MQ=255)
                                                                        cacaTTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATgg                <  2:47963/93‑1 (MQ=255)
                                                                         acaTTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGt               >  2:4567036/1‑93 (MQ=255)
                                                                          caTTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTc              <  1:4998358/93‑1 (MQ=255)
                                                                                ttATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATc        >  2:3142810/1‑93 (MQ=255)
                                                                                 tATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCa       >  2:1794862/1‑93 (MQ=255)
                                                                                 tATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCa       <  1:3412603/93‑1 (MQ=255)
                                                                                  aTCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAg      >  1:276671/1‑93 (MQ=255)
                                                                                   tCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAgg     <  1:2510517/93‑1 (MQ=255)
                                                                                   tCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAgg     <  2:6134614/93‑1 (MQ=255)
                                                                                   tCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAgg     <  1:1287938/93‑1 (MQ=255)
                                                                                    cccAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGt    <  1:5522460/93‑1 (MQ=255)
                                                                                     ccAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTc   >  1:549705/1‑93 (MQ=255)
                                                                                         |                                                                                         
GGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCG  >  NC_000913/2133715‑2133893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGT  >  NC_000913/2133712‑2133897
                                                                                            |                                                                                             
GGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCG                                                                                              <  SRR4302533.395242/94‑1 (MQ=60)
  CGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGAC                                                                                            >  SRR4302533.2584723/1‑94 (MQ=60)
  CGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGAC                                                                                            >  SRR4302533.619971/1‑94 (MQ=60)
   GGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCTAGCGACC                                                                                           >  SRR4302533.1465039/1‑94 (MQ=60)
     GCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAA                                                                                         <  SRR4302533.5271890/94‑1 (MQ=60)
      CAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGAGTTCACATTCGTTATCCCAGCGACCCAA                                                                                        >  SRR4302533.161335/1‑94 (MQ=60)
       AGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTTACATTCGTTATCCCAGCGACCAAAC                                                                                       >  SRR4302533.1206851/1‑94 (MQ=60)
       AGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAAC                                                                                       >  SRR4302533.4504669/1‑94 (MQ=60)
       AGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAAC                                                                                       >  SRR4302533.5284430/1‑94 (MQ=60)
       AGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAAC                                                                                       <  SRR4302533.1872916/94‑1 (MQ=60)
       AGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAAC                                                                                       >  SRR4302533.1946125/1‑94 (MQ=60)
       AGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAAC                                                                                       >  SRR4302533.3972066/1‑94 (MQ=60)
         ACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAG                                                                                     >  SRR4302533.347416/1‑94 (MQ=60)
          CCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGC                                                                                    <  SRR4302533.5765810/94‑1 (MQ=60)
          CCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGC                                                                                    <  SRR4302533.5593503/94‑1 (MQ=60)
          CCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGC                                                                                    >  SRR4302533.5408492/1‑94 (MQ=60)
          CCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGC                                                                                    <  SRR4302533.1075274/94‑1 (MQ=60)
          CCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTATGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGC                                                                                    >  SRR4302533.3400335/1‑94 (MQ=60)
           CGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCA                                                                                   >  SRR4302533.3389948/1‑94 (MQ=60)
           CGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCA                                                                                   <  SRR4302533.915638/94‑1 (MQ=60)
             TTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATA                                                                                 >  SRR4302533.4158270/1‑94 (MQ=60)
              TCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCA                                                                                >  SRR4302533.524988/1‑94 (MQ=60)
              TCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCA                                                                                >  SRR4302533.4844224/1‑94 (MQ=60)
              TCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCA                                                                                <  SRR4302533.2734498/94‑1 (MQ=60)
              TCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCA                                                                                <  SRR4302533.2222860/94‑1 (MQ=60)
               CAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCAC                                                                               >  SRR4302533.4510880/1‑94 (MQ=60)
                AAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACT                                                                              <  SRR4302533.2478780/94‑1 (MQ=60)
                 AGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTT                                                                             <  SRR4302533.3679767/94‑1 (MQ=60)
                 AGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTT                                                                             <  SRR4302533.4340665/94‑1 (MQ=60)
                 AGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTT                                                                             <  SRR4302533.2157113/94‑1 (MQ=60)
                  GTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTT                                                                            <  SRR4302533.1641617/94‑1 (MQ=60)
                   TAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTG                                                                           <  SRR4302533.112965/94‑1 (MQ=60)
                   TAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTG                                                                           <  SRR4302533.3329578/94‑1 (MQ=60)
                   TAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTG                                                                           <  SRR4302533.460168/94‑1 (MQ=60)
                   TAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTG                                                                           <  SRR4302533.6026836/94‑1 (MQ=60)
                     ATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCC                                                                         >  SRR4302533.1439252/1‑94 (MQ=60)
                     ATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCC                                                                         <  SRR4302533.5149186/94‑1 (MQ=60)
                       GTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGC                                                                       >  SRR4302533.2962825/1‑94 (MQ=60)
                        TGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCG                                                                      >  SRR4302533.4592483/1‑94 (MQ=60)
                          TACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCA                                                                    >  SRR4302533.273336/1‑94 (MQ=60)
                          TACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCA                                                                    >  SRR4302533.6244343/1‑94 (MQ=60)
                          TACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGACGCGCA                                                                    >  SRR4302533.3882881/1‑94 (MQ=60)
                             ACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCT                                                                 >  SRR4302533.2903360/1‑94 (MQ=60)
                                GCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCG                                                              <  SRR4302533.4598918/94‑1 (MQ=60)
                                 CTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGG                                                             <  SRR4302533.1356028/94‑1 (MQ=60)
                                 CTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGG                                                             >  SRR4302533.4014073/1‑94 (MQ=60)
                                   GCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTG                                                           >  SRR4302533.476902/1‑94 (MQ=60)
                                   GCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTG                                                           <  SRR4302533.826224/94‑1 (MQ=60)
                                    CAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCCGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGC                                                          >  SRR4302533.4709546/1‑94 (MQ=60)
                                    CAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGC                                                          >  SRR4302533.1633488/1‑94 (MQ=60)
                                     AAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCC                                                         >  SRR4302533.3384312/1‑94 (MQ=60)
                                     AAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCC                                                         >  SRR4302533.2682390/1‑94 (MQ=60)
                                     AAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCC                                                         >  SRR4302533.4453456/1‑94 (MQ=60)
                                     AAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCC                                                         >  SRR4302533.4482643/1‑94 (MQ=60)
                                      AACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAatacagcagatcggaagagcggttcagcag                                                        >  SRR4302533.923186/1‑64 (MQ=60)
                                      AACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCA                                                        <  SRR4302533.340127/94‑1 (MQ=60)
                                      AACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCA                                                        >  SRR4302533.1857687/1‑94 (MQ=60)
                                      AACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCA                                                        >  SRR4302533.1887624/1‑94 (MQ=60)
                                       ACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAT                                                       >  SRR4302533.4502893/1‑94 (MQ=60)
                                        CGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATC                                                      >  SRR4302533.2444309/1‑94 (MQ=60)
                                         GTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCT                                                     <  SRR4302533.4965517/94‑1 (MQ=60)
                                          TTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTC                                                    >  SRR4302533.5815642/1‑94 (MQ=60)
                                          TTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTC                                                    <  SRR4302533.5207299/94‑1 (MQ=60)
                                           TTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCG                                                   <  SRR4302533.2992782/94‑1 (MQ=60)
                                           TTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCG                                                   <  SRR4302533.1843419/94‑1 (MQ=60)
                                             CCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCA                                                 >  SRR4302533.106365/1‑94 (MQ=60)
                                             CCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCA                                                 >  SRR4302533.4306654/1‑94 (MQ=60)
                                             CCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCA                                                 >  SRR4302533.4762292/1‑94 (MQ=60)
                                                 GCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAG                                             <  SRR4302533.3990702/94‑1 (MQ=60)
                                                  CTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGT                                            >  SRR4302533.3064560/1‑94 (MQ=60)
                                                  CTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGC                                            <  SRR4302533.1552589/94‑1 (MQ=60)
                                                  CTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGC                                            >  SRR4302533.6024613/1‑94 (MQ=60)
                                                  CTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGC                                            <  SRR4302533.3393934/94‑1 (MQ=60)
                                                  CTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGC                                            >  SRR4302533.3683121/1‑94 (MQ=60)
                                                  CTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGC                                            >  SRR4302533.3430297/1‑94 (MQ=60)
                                                  CTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCCGATAAGC                                            >  SRR4302533.6010666/1‑94 (MQ=60)
                                                   TTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCG                                           <  SRR4302533.3384806/94‑1 (MQ=60)
                                                    TTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGT                                          <  SRR4302533.3144542/94‑1 (MQ=60)
                                                    TTTGCGATACGGATCGGGGACTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGT                                          <  SRR4302533.4229749/94‑1 (MQ=60)
                                                     TTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGGATCACTTTGGCGTGCATCTCCGGTGCGATCTCGCATAAGCGTT                                         >  SRR4302533.169536/1‑94 (MQ=60)
                                                      TGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTC                                        <  SRR4302533.2686294/94‑1 (MQ=60)
                                                      TGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTC                                        <  SRR4302533.4377417/94‑1 (MQ=60)
                                                      TGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTC                                        <  SRR4302533.1306708/94‑1 (MQ=60)
                                                        CGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGA                                      >  SRR4302533.3005804/1‑94 (MQ=60)
                                                        CGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGA                                      <  SRR4302533.4551228/94‑1 (MQ=60)
                                                         GATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGAT                                     >  SRR4302533.3294835/1‑94 (MQ=60)
                                                         GATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGAT                                     <  SRR4302533.238651/94‑1 (MQ=60)
                                                          ATACGGATCGGTGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCTCTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATA                                    <  SRR4302533.2155776/94‑1 (MQ=60)
                                                          ATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATCAGCGTTCGATA                                    >  SRR4302533.5883914/1‑94 (MQ=60)
                                                          ATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATA                                    >  SRR4302533.5633251/1‑94 (MQ=60)
                                                          ATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATA                                    >  SRR4302533.629546/1‑94 (MQ=60)
                                                           TACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATAT                                   >  SRR4302533.5503699/1‑94 (MQ=60)
                                                           TACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATAT                                   <  SRR4302533.5068248/94‑1 (MQ=60)
                                                            ACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATG                                  <  SRR4302533.5960602/94‑1 (MQ=60)
                                                             CGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGG                                 <  SRR4302533.413678/94‑1 (MQ=60)
                                                              GGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGC                                <  SRR4302533.1741402/94‑1 (MQ=60)
                                                                  CGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTT                            >  SRR4302533.4699576/1‑94 (MQ=60)
                                                                   GGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTT                           >  SRR4302533.1397077/1‑94 (MQ=60)
                                                                   GGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTT                           <  SRR4302533.835917/94‑1 (MQ=60)
                                                                   GGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTT                           >  SRR4302533.3517743/1‑94 (MQ=60)
                                                                    GGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTT                          <  SRR4302533.191336/94‑1 (MQ=60)
                                                                    GGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTT                          >  SRR4302533.3900223/1‑94 (MQ=60)
                                                                    GGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTT                          >  SRR4302533.3492000/1‑94 (MQ=60)
                                                                     GGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATCAGCGTTCGATATGGCGCTTTTC                         >  SRR4302533.3795935/1‑94 (MQ=60)
                                                                     GGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTC                         >  SRR4302533.5276454/1‑94 (MQ=60)
                                                                      GATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCC                        >  SRR4302533.3307164/1‑94 (MQ=60)
                                                                       ATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATTTCGCATAAGCGTTCGATATGGCGCTTTTCCA                       >  SRR4302533.1824037/1‑94 (MQ=60)
                                                                       ATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCA                       >  SRR4302533.2684728/1‑94 (MQ=60)
                                                                       ATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCA                       <  SRR4302533.715849/94‑1 (MQ=60)
                                                                          TCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGG                    >  SRR4302533.4708498/1‑94 (MQ=60)
                                                                           CACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGT                   >  SRR4302533.5542624/1‑94 (MQ=60)
                                                                           CACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGT                   <  SRR4302533.3527393/94‑1 (MQ=60)
                                                                            ACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTC                  <  SRR4302533.545274/94‑1 (MQ=60)
                                                                            ACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTC                  >  SRR4302533.4979597/1‑94 (MQ=60)
                                                                             CATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCA                 >  SRR4302533.1723372/1‑94 (MQ=60)
                                                                             CATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCA                 <  SRR4302533.5028660/94‑1 (MQ=60)
                                                                             CATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCA                 >  SRR4302533.1227604/1‑94 (MQ=60)
                                                                             CATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCA                 <  SRR4302533.2651272/94‑1 (MQ=60)
                                                                                TCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAA              >  SRR4302533.1966088/1‑94 (MQ=60)
                                                                                 CGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAAT             <  SRR4302533.1529209/94‑1 (MQ=60)
                                                                                  GTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATC            <  SRR4302533.6200156/94‑1 (MQ=60)
                                                                                  GTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATC            >  SRR4302533.319615/1‑94 (MQ=60)
                                                                                   TTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGAGCTTTTCCATGGTCAAAATCA           >  SRR4302533.6235834/1‑94 (MQ=60)
                                                                                    TATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAG          <  SRR4302533.3433682/94‑1 (MQ=60)
                                                                                    TATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAG          >  SRR4302533.233654/1‑94 (MQ=60)
                                                                                    TATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAG          <  SRR4302533.4836045/94‑1 (MQ=60)
                                                                                    TATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAG          >  SRR4302533.278355/1‑94 (MQ=60)
                                                                                      TCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGT        <  SRR4302533.2525989/94‑1 (MQ=60)
                                                                                      TCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGT        >  SRR4302533.5207145/1‑94 (MQ=60)
                                                                                      TCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGT        <  SRR4302533.1295686/94‑1 (MQ=60)
                                                                                       CCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTC       <  SRR4302533.5555805/94‑1 (MQ=60)
                                                                                       CCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTC       >  SRR4302533.552969/1‑94 (MQ=60)
                                                                                        CCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCG      >  SRR4302533.50540/1‑94 (MQ=60)
                                                                                           GCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAG   >  SRR4302533.6330368/1‑94 (MQ=60)
                                                                                            CGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGT  >  SRR4302533.4440344/1‑94 (MQ=60)
                                                                                            |                                                                                             
GGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGT  >  NC_000913/2133712‑2133897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑