Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,284,989	(C)_5→4	coding (1932/2511 nt)	yejO ←	putative ATP‑binding component of a transport system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,284,985	0	C	.	99.2%	1107.7 / ‑0.6	247	coding (1936/2511 nt)	yejO	putative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand): ref base C (1/1); new base . (116/129); total (117/130)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

Only 100 of 275 total aligned reads displayed.

CTCATCTTGCCATGGATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAGCCCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGAATT  >  NC_000913/2284894‑2285073

    tCTTGCCATGGATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAGccccag                                                                                     <  1:10035666/93‑3 (MQ=255)

        gCCATGGATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGta                                                                                >  2:5072836/1‑93 (MQ=255)

         ccATGGATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGtat                                                                               <  1:2017067/93‑1 (MQ=255)

          cATGGATGGTGTTTGCAAAACGGTCCACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGtata                                                                              <  1:7801744/93‑1 (MQ=255)

          cATGGATGGTGTTGGCAAAACGGTCAACTTTCACCACCTCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGtata                                                                              <  2:3415204/93‑1 (MQ=255)

          cATGGATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCGAACCGGCATAAG‑CCCCAGGGtata                                                                              <  2:665538/93‑1 (MQ=255)

           atggatggTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAg                                                                             >  2:8038187/1‑93 (MQ=255)

             ggatggTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCt                                                                           <  2:8905648/93‑1 (MQ=255)

             ggatggTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCt                                                                           <  2:4697726/93‑1 (MQ=255)

             ggatggTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCt                                                                           <  1:4062767/93‑1 (MQ=255)

              gatggTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTa                                                                          >  2:228619/1‑93 (MQ=255)

              gatggTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTa                                                                          <  2:7419499/93‑1 (MQ=255)

               atggTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTAt                                                                         <  2:6088513/93‑1 (MQ=255)

               atggTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTAt                                                                         >  2:8128312/1‑93 (MQ=255)

                tggTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATc                                                                        >  1:4075077/1‑93 (MQ=255)

                 ggTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCg                                                                       <  2:2756754/93‑1 (MQ=255)

                 ggTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCg                                                                       >  2:8501750/1‑93 (MQ=255)

                  gtgtTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGa                                                                      <  2:4227155/93‑1 (MQ=255)

                    gtTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGata                                                                    <  1:3142921/93‑1 (MQ=255)

                    gtTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGata                                                                    >  2:938340/1‑93 (MQ=255)

                      tGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATAtt                                                                  <  1:1998482/93‑1 (MQ=255)

                          aaaaCGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCt                                                              <  2:2336417/93‑1 (MQ=255)

                           aaaTGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCtt                                                             <  1:3358218/93‑1 (MQ=255)

                           aaaCGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCtt                                                             >  2:6311836/1‑93 (MQ=255)

                               cgTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTgcc                                                         <  1:5267134/92‑1 (MQ=255)

                                 tCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTgccgc                                                       >  1:981848/1‑93 (MQ=255)

                                 tCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTgccgc                                                       <  2:1854425/93‑1 (MQ=255)

                                 tCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTgccgc                                                       <  2:6455005/93‑1 (MQ=255)

                                 tCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTgccgc                                                       <  1:1115341/93‑1 (MQ=255)

                                 tCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTgccgc                                                       >  1:4473080/1‑93 (MQ=255)

                                  taaCTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTgccgcc                                                      <  2:7334410/92‑1 (MQ=255)

                                    aCTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCcgccgc                                                    <  2:6573303/93‑1 (MQ=255)

                                     cTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCcgcg                                                   >  1:4227393/1‑93 (MQ=255)

                                      tttCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGa                                                  <  1:6429661/93‑1 (MQ=255)

                                      tttCACCACCCCATCAACATAGGCACCGTTCTGATGCCCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGa                                                  <  1:5489529/93‑1 (MQ=255)

                                       ttCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGAt                                                 >  2:6563508/1‑93 (MQ=255)

                                        tCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATc                                                >  2:5810201/1‑93 (MQ=255)

                                          accaccCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCaa                                              >  2:150852/1‑93 (MQ=255)

                                          accaccCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCaa                                              >  1:8831894/1‑93 (MQ=255)

                                            caccCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCaaaa                                            >  2:8338751/1‑93 (MQ=255)

                                            caccCCATCAACATAGGCACCGTGCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCaaaa                                            <  1:2424706/93‑1 (MQ=255)

                                             accCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAAc                                           >  2:4362224/1‑93 (MQ=255)

                                              ccccATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAAcc                                          >  1:7785548/1‑93 (MQ=255)

                                               cccATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCa                                         >  1:2667680/1‑93 (MQ=255)

                                                ccATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCaa                                        >  2:3630481/1‑93 (MQ=255)

                                                   tCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAatat                                     >  2:2757879/1‑93 (MQ=255)

                                                   tCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAatat                                     >  1:3517740/1‑93 (MQ=255)

                                                   tCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAatat                                     >  1:1047017/1‑93 (MQ=255)

                                                    cAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATc                                    <  1:2795704/93‑1 (MQ=255)

                                                    cAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATc                                    <  1:8545825/93‑1 (MQ=255)

                                                     aaCATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGGATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCa                                   >  2:1989926/1‑93 (MQ=255)

                                                      acacAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAg                                  <  2:9257155/93‑1 (MQ=255)

                                                        aTAGGCACCGTTCTGATGCTCCCTACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGaa                                <  2:5507959/93‑1 (MQ=255)

                                                        aTAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGaa                                <  2:1177861/93‑1 (MQ=255)

                                                        aTAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGaa                                <  1:1173110/93‑1 (MQ=255)

                                                        aTAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGaa                                >  1:2434492/1‑93 (MQ=255)

                                                        aTAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGaa                                >  1:9159060/1‑93 (MQ=255)

                                                        aTAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGaa                                <  1:5197792/93‑1 (MQ=255)

                                                        aTAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGaa                                <  1:6983145/93‑1 (MQ=255)

                                                         tAGGCACCGTCCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAAt                               <  2:7805477/93‑1 (MQ=255)

                                                            gCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATgag                            >  1:1237641/1‑93 (MQ=255)

                                                            gCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATgag                            >  1:4883701/1‑93 (MQ=255)

                                                             cACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATgaga                           <  1:6106084/93‑1 (MQ=255)

                                                             cACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATgaga                           <  1:8747388/93‑1 (MQ=255)

                                                             cACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATgaga                           <  2:9151284/93‑1 (MQ=255)

                                                              aCCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGagag                          <  1:6466361/93‑1 (MQ=255)

                                                              aCCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGagag                          >  2:3085482/1‑93 (MQ=255)

                                                               ccGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGt                         >  2:380502/1‑93 (MQ=255)

                                                                  ttCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAAc                      >  2:822732/1‑93 (MQ=255)

                                                                  ttCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAAc                      <  1:468216/93‑1 (MQ=255)

                                                                  ttCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAAc                      >  1:1609642/1‑93 (MQ=255)

                                                                  ttCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAAc                      >  1:813588/1‑93 (MQ=255)

                                                                  ttCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAAc                      >  2:2103315/1‑93 (MQ=255)

                                                                   tCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTGCCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAAcc                     <  1:5405741/93‑1 (MQ=255)

                                                                   tCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAAcc                     >  1:5824616/1‑93 (MQ=255)

                                                                     tGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCaa                   <  2:7141858/93‑1 (MQ=255)

                                                                     tGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCaa                   >  1:2969515/1‑93 (MQ=255)

                                                                     tGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCaa                   >  2:8775790/1‑93 (MQ=255)

                                                                     tGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCaa                   >  1:7421165/1‑93 (MQ=255)

                                                                       aTGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAg                 <  2:6876603/93‑1 (MQ=255)

                                                                       aTGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAg                 <  1:7896451/93‑1 (MQ=255)

                                                                         gCTCCCAACCGGCATAAGCCCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGa                <  1:8963132/93‑1 (MQ=255)

                                                                         gCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTa                        >  1:4340938/1‑84 (MQ=255)

                                                                         gCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGAt               <  2:1652618/93‑1 (MQ=255)

                                                                           tCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCa             >  1:3779644/1‑93 (MQ=255)

                                                                            cccAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGCTCAAAACCAATATCAGAATGAGAGTAAGCAAAGATCaa            >  2:5205792/1‑93 (MQ=255)

                                                                             ccAAGCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAg           >  2:3446272/1‑93 (MQ=255)

                                                                             ccAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAg           <  1:1323023/93‑1 (MQ=255)

                                                                              cAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGc          <  2:6035814/93‑1 (MQ=255)

                                                                               aaCCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGcc         >  2:2471394/1‑93 (MQ=255)

                                                                                aCCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCcg        >  2:9645700/1‑93 (MQ=255)

                                                                                aCCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCcg        >  1:2305227/1‑93 (MQ=255)

                                                                                 ccGGCATAAG‑CCCCAGGGTATAGCTATTGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCcgc       <  2:1043141/93‑1 (MQ=255)

                                                                                 ccGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCcgc       <  2:48554/93‑1 (MQ=255)

                                                                                    gCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGaa    >  1:3711861/1‑93 (MQ=255)

                                                                                    gCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGaa    >  1:967463/1‑93 (MQ=255)

                                                                                     cATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGAAt   >  1:6820387/1‑93 (MQ=255)

                                                                                     cATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGAAt   >  2:1421823/1‑93 (MQ=255)

                                                                                      aTAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGAAtt  <  2:8376111/93‑1 (MQ=255)

                                                                                      aTAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGAAtt  <  2:4335823/93‑1 (MQ=255)

CTCATCTTGCCATGGATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAGCCCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGAATT  >  NC_000913/2284894‑2285073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCTTGCCATGGATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAGCCCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGAATTGTACTGCT  >  NC_000913/2284898‑2285081

TCTTGCCATGGATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGG                                                                                           <  SRR4302540.9692402/94‑1 (MQ=60)

TCTTGCCATGGATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGG                                                                                           <  SRR4302540.10152382/94‑1 (MQ=60)

 CTTGCCATGGATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGG                                                                                          >  SRR4302540.8809287/1‑94 (MQ=60)

  TTGCCATGGATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGT                                                                                         <  SRR4302540.7737810/94‑1 (MQ=60)

  TTGCCATGGATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGT                                                                                         <  SRR4302540.507183/94‑1 (MQ=60)

    GCCATGGATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCTTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTAT                                                                                       <  SRR4302540.4843485/94‑1 (MQ=60)

    GCCATGGATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTAT                                                                                       <  SRR4302540.8954369/94‑1 (MQ=60)

    GCCATGGATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTAT                                                                                       <  SRR4302540.1092370/94‑1 (MQ=60)

    GCCATGGATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTAT                                                                                       <  SRR4302540.4215148/94‑1 (MQ=60)

    GCCATGGATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTAT                                                                                       <  SRR4302540.4843486/94‑1 (MQ=60)

     CCATGGATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATA                                                                                      <  SRR4302540.2038875/94‑1 (MQ=60)

     CCATGGATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATA                                                                                      <  SRR4302540.536812/94‑1 (MQ=60)

      CATGGATGGTGTTTGCAAAACGGTCCACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAG                                                                                     <  SRR4302540.7892927/94‑1 (MQ=60)

      CATGGATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAG                                                                                     <  SRR4302540.7892926/94‑1 (MQ=60)

      CATGGATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAG                                                                                     >  SRR4302540.6043776/1‑94 (MQ=60)

      CATGGATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGACATAAG‑CCCCAGGGTATAG                                                                                     >  SRR4302540.1027172/1‑94 (MQ=60)

       ATGGATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGC                                                                                    >  SRR4302540.5314424/1‑94 (MQ=60)

       ATGGATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGC                                                                                    >  SRR4302540.3777614/1‑94 (MQ=60)

       ATGGATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGC                                                                                    >  SRR4302540.3004739/1‑94 (MQ=60)

         GGATGGTGTTGGCAGAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTA                                                                                  <  SRR4302540.4106577/94‑1 (MQ=60)

         GGATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTA                                                                                  <  SRR4302540.4106575/94‑1 (MQ=60)

           ATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATC                                                                                >  SRR4302540.4119003/1‑94 (MQ=60)

            TGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCG                                                                               <  SRR4302540.889754/94‑1 (MQ=60)

              GTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGAT                                                                             <  SRR4302540.6515816/94‑1 (MQ=60)

              GTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGAT                                                                             <  SRR4302540.6515817/94‑1 (MQ=60)

              GTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGAT                                                                             >  SRR4302540.3804372/1‑94 (MQ=60)

              GTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGAT                                                                             >  SRR4302540.4691252/1‑94 (MQ=60)

                GTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATAT                                                                           >  SRR4302540.99368/1‑94 (MQ=60)

                GTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATAT                                                                           <  SRR4302540.3177182/94‑1 (MQ=60)

                  TGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTA                                                                         <  SRR4302540.2020129/94‑1 (MQ=60)

                   GGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTAC                                                                        <  SRR4302540.250502/94‑1 (MQ=60)

                     CAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCT                                                                      <  SRR4302540.1154177/94‑1 (MQ=60)

                      AAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTT                                                                     >  SRR4302540.1258727/1‑94 (MQ=60)

                       AAATGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTT                                                                    <  SRR4302540.3395000/94‑1 (MQ=60)

                       AAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTT                                                                    <  SRR4302540.3696456/94‑1 (MQ=60)

                       AAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTT                                                                    >  SRR4302540.8407715/1‑94 (MQ=60)

                           CGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCG                                                                <  SRR4302540.5324617/94‑1 (MQ=60)

                            GTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGC                                                               >  SRR4302540.991902/1‑94 (MQ=60)

                            GTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGC                                                               >  SRR4302540.4521595/1‑94 (MQ=60)

                             TCTACTCTCACTACCCCATCAACATAGGCATCGGTCTGATGCTCCCAATCGGTATAAG‑CCCCAGGGTATAGCTATCGATAATACCTTTGCCGCC                                                              >  SRR4302540.9093321/1‑94 (MQ=54)

                             TCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCC                                                              <  SRR4302540.1126896/94‑1 (MQ=60)

                             TCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCC                                                              <  SRR4302540.2134553/94‑1 (MQ=60)

                               AACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGC                                                            <  SRR4302540.1143185/94‑1 (MQ=60)

                                ACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCG                                                           >  SRR4302540.4273416/1‑94 (MQ=60)

                                ACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCG                                                           >  SRR4302540.4273415/1‑94 (MQ=60)

                                ACTTTCACCACCCCATCAACAGAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCG                                                           <  SRR4302540.4126585/94‑1 (MQ=60)

                                  TTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGAT                                                         <  SRR4302540.6501227/94‑1 (MQ=60)

                                  TTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGAT                                                         >  SRR4302540.7910241/1‑94 (MQ=60)

                                  TTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGAT                                                         >  SRR4302540.8124634/1‑94 (MQ=60)

                                  TTTCACCACCCCATCAACATAGGCACCGTTCTGATGCCCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGAT                                                         <  SRR4302540.5549960/94‑1 (MQ=60)

                                    TCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCA                                                       >  SRR4302540.2456513/1‑94 (MQ=60)

                                     CACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAA                                                      <  SRR4302540.4066784/94‑1 (MQ=60)

                                     CACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAA                                                      >  SRR4302540.8934038/1‑94 (MQ=60)

                                       CCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAA                                                    >  SRR4302540.1198288/1‑94 (MQ=60)

                                        CACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAAC                                                   <  SRR4302540.2450828/94‑1 (MQ=60)

                                        CACCCCATCAACATAGGCACCGTGCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAAC                                                   <  SRR4302540.2450827/94‑1 (MQ=60)

                                         ACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACC                                                  >  SRR4302540.7876553/1‑94 (MQ=60)

                                          CCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAACACCA                                                 >  SRR4302540.4389741/1‑94 (MQ=60)

                                          CCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCA                                                 >  SRR4302540.2696588/1‑94 (MQ=60)

                                           CCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAA                                                >  SRR4302540.8270550/1‑94 (MQ=60)

                                            CCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAAT                                               <  SRR4302540.889817/94‑1 (MQ=60)

                                             CATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATA                                              <  SRR4302540.326608/94‑1 (MQ=60)

                                              ATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATAT                                             >  SRR4302540.3556066/1‑94 (MQ=60)

                                              ATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATAT                                             >  SRR4302540.1057658/1‑94 (MQ=60)

                                               TCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATC                                            >  SRR4302540.3381256/1‑94 (MQ=60)

                                                CAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCA                                           >  SRR4302540.1188997/1‑94 (MQ=60)

                                                CAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCA                                           <  SRR4302540.6782177/94‑1 (MQ=60)

                                                CAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCA                                           <  SRR4302540.8645110/94‑1 (MQ=60)

                                                CAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCA                                           <  SRR4302540.2825891/94‑1 (MQ=60)

                                                 AACATAGGCAGCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAG                                          <  SRR4302540.1344256/94‑1 (MQ=60)

                                                 AACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAG                                          <  SRR4302540.982178/94‑1 (MQ=60)

                                                 AACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAG                                          <  SRR4302540.2540263/94‑1 (MQ=60)

                                                  ACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGA                                         <  SRR4302540.2035970/94‑1 (MQ=60)

                                                   CATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAA                                        >  SRR4302540.2460718/1‑94 (MQ=60)

                                                   CATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAA                                        >  SRR4302540.9268998/1‑94 (MQ=60)

                                                    atatggacccgtcgtATGCTCCCAACCGGCACAAG‑CCGCAGGGTATAGCTATCGATATTTCCTTTGCCGCCGCGATCAAAACCAATATCAGAAT                                       <  SRR4302540.2984761/79‑1 (MQ=60)

                                                    ATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAAT                                       <  SRR4302540.5254395/94‑1 (MQ=60)

                                                    ATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAAT                                       <  SRR4302540.1185247/94‑1 (MQ=60)

                                                    ATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAAT                                       <  SRR4302540.696486/94‑1 (MQ=60)

                                                    ATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAAT                                       <  SRR4302540.7060699/94‑1 (MQ=60)

                                                    ATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAAT                                       <  SRR4302540.2970310/94‑1 (MQ=60)

                                                    ATAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAAT                                       >  SRR4302540.5204205/1‑94 (MQ=60)

                                                     TAGGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATG                                      <  SRR4302540.5931000/94‑1 (MQ=60)

                                                      AGGCACCGTTCTGATGCTCCCAACCGGCATAAGCCCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATG                                      >  SRR4302540.1927223/1‑94 (MQ=60)

                                                       GGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAG                                    >  SRR4302540.1250276/1‑94 (MQ=60)

                                                       GGCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAG                                    >  SRR4302540.4936159/1‑94 (MQ=60)

                                                        GCACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGA                                   <  SRR4302540.3747015/94‑1 (MQ=60)

                                                         CACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAG                                  <  SRR4302540.8848897/94‑1 (MQ=60)

                                                         CACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAG                                  <  SRR4302540.3026957/94‑1 (MQ=60)

                                                         CACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAG                                  <  SRR4302540.6174089/94‑1 (MQ=60)

                                                          ACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGT                                 <  SRR4302540.6538319/94‑1 (MQ=60)

                                                          ACCGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGCATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGT                                 >  SRR4302540.1563937/1‑94 (MQ=60)

                                                            CGTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAA                               >  SRR4302540.5705301/1‑94 (MQ=60)

                                                             GTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAAC                              >  SRR4302540.1627272/1‑94 (MQ=60)

                                                             GTTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAAC                              >  SRR4302540.822228/1‑94 (MQ=60)

                                                              TTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACC                             >  SRR4302540.5889004/1‑94 (MQ=60)

                                                              TTCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACC                             <  SRR4302540.473224/94‑1 (MQ=60)

                                                               TCTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTGCCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCA                            <  SRR4302540.5465179/94‑1 (MQ=60)

                                                                CTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGCTCAAAACCAATATCAGAATGAGAGAAACCAA                           >  SRR4302540.7503824/1‑94 (MQ=60)

                                                                CTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAA                           >  SRR4302540.3002313/1‑94 (MQ=60)

                                                                CTGATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAA                           >  SRR4302540.7503825/1‑94 (MQ=60)

                                                                   ATGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGA                        <  SRR4302540.7988578/94‑1 (MQ=60)

                                                                    TGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAAaagcgcta                       >  SRR4302540.4388196/1‑86 (MQ=60)

                                                                    TGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGAT                       <  SRR4302540.7565449/94‑1 (MQ=60)

                                                                    TGCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGAT                       <  SRR4302540.4401124/94‑1 (MQ=60)

                                                                     GCTCCCAACCGGCATAAGCCCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGAT                       <  SRR4302540.9069254/94‑1 (MQ=60)

                                                                     GCTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATC                      <  SRR4302540.5868727/94‑1 (MQ=60)

                                                                      CTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCA                     >  SRR4302540.3820875/1‑94 (MQ=60)

                                                                      CTCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCA                     <  SRR4302540.1114648/94‑1 (MQ=60)

                                                                       TCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAA                    >  SRR4302540.2728625/1‑94 (MQ=60)

                                                                       TCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCGTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAA                    <  SRR4302540.1807841/94‑1 (MQ=60)

                                                                       CCCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAA                    <  SRR4302540.3988025/94‑1 (MQ=60)

                                                                        CCCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAG                   >  SRR4302540.8774295/1‑94 (MQ=60)

                                                                         CCAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGC                  <  SRR4302540.1336814/94‑1 (MQ=60)

                                                                          CAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCC                 <  SRR4302540.5524295/94‑1 (MQ=60)

                                                                          CAACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCC                 >  SRR4302540.2414481/1‑94 (MQ=60)

                                                                           AACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCG                <  SRR4302540.9567803/94‑1 (MQ=60)

                                                                           AACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCG                >  SRR4302540.2330029/1‑94 (MQ=60)

                                                                           AACCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCG                <  SRR4302540.5532989/94‑1 (MQ=60)

                                                                             CCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCG              <  SRR4302540.7981628/94‑1 (MQ=60)

                                                                             CCGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCG              <  SRR4302540.290562/94‑1 (MQ=60)

                                                                              CGGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGA             >  SRR4302540.4373222/1‑94 (MQ=60)

                                                                               GGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGAA            >  SRR4302540.977441/1‑94 (MQ=60)

                                                                               GGCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGAA            >  SRR4302540.3752367/1‑94 (MQ=60)

                                                                                GCATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGAAT           >  SRR4302540.6895889/1‑94 (MQ=60)

                                                                                 CATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGAATT          <  SRR4302540.2020416/94‑1 (MQ=60)

                                                                                  ATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGAATTG         >  SRR4302540.1710431/1‑94 (MQ=60)

                                                                                  ATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGAATTG         >  SRR4302540.7651371/1‑94 (MQ=60)

                                                                                  ATAAG‑CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGAATTG         >  SRR4302540.920631/1‑94 (MQ=60)

                                                                                      agCCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGAATTGTAC      <  SRR4302540.5640525/92‑1 (MQ=60)

                                                                                       GCCCCAGGGTATAGCTATCTATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGAATTGTACT     <  SRR4302540.9891817/94‑1 (MQ=60)

                                                                                       GCCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGAATTGTACT     <  SRR4302540.8969747/94‑1 (MQ=60)

                                                                                       GCCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGAATTGTACT     >  SRR4302540.3593267/1‑94 (MQ=60)

                                                                                        CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGAATTGTACTG    <  SRR4302540.1058218/94‑1 (MQ=60)

                                                                                        CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGAATTGTACTG    >  SRR4302540.7367371/1‑94 (MQ=60)

                                                                                        CCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGAATTGTACTG    >  SRR4302540.8206294/1‑94 (MQ=60)

                                                                                         CCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAGCCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGAATTGTACTGC   <  SRR4302540.113046/94‑1 (MQ=60)

                                                                                         CCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGAATTGTACTGT   >  SRR4302540.8641836/1‑94 (MQ=60)

                                                                                          CCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGAATTGTACTGCT  >  SRR4302540.6936316/1‑94 (MQ=60)

                                                                                          CCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGAATTGTACTGCT  >  SRR4302540.5468041/1‑94 (MQ=60)

TCTTGCCATGGATGGTGTTGGCAAAACGGTCAACTTTCACCACCCCATCAACATAGGCACCGTTCTGATGCTCCCAACCGGCATAAGCCCCCAGGGTATAGCTATCGATATTACCTTTGCCGCCGCGATCAAAACCAATATCAGAATGAGAGTAACCAAAGATCAAGCCGCGAATTGTACTGCT  >  NC_000913/2284898‑2285081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`