Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,327,687	A→G	V44A (GTG→GCG)	b2226 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,327,687	0	A	G	100.0%	784.1 / NA	243	V44A (GTG→GCG)	b2226	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (145/98); total (145/98)

Only 100 of 252 total aligned reads displayed.

TGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGA  >  NC_000913/2327597‑2327776

tGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTcgcg                                                                                         >  1:2796203/1‑93 (MQ=255)

    tATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCtg                                                                                     >  2:2771429/1‑93 (MQ=255)

    gaTCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCtg                                                                                     <  2:4601927/92‑1 (MQ=255)

     aTCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCtga                                                                                    >  1:9473294/1‑93 (MQ=255)

     aTCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCtga                                                                                    <  2:8031020/93‑1 (MQ=255)

      tcctccAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCtgat                                                                                   >  1:4920115/1‑93 (MQ=255)

      tcctccAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCtgat                                                                                   >  1:5003112/1‑93 (MQ=255)

      tcctccAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCtgat                                                                                   <  2:1138814/93‑1 (MQ=255)

      tcctccAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCtgat                                                                                   <  2:9404868/93‑1 (MQ=255)

        ctccAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCtgatga                                                                                 <  1:6627842/93‑1 (MQ=255)

         tccAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATgac                                                                                >  1:13909/1‑93 (MQ=255)

         tccAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATgac                                                                                >  2:4124156/1‑93 (MQ=255)

         cccagccagGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATgac                                                                                <  2:1380852/92‑1 (MQ=255)

          ccagccagGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATgacg                                                                               >  2:6531966/1‑93 (MQ=255)

           cagccagGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATgacga                                                                              <  1:2352327/93‑1 (MQ=255)

             gccagGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACa                                                                            <  2:6304710/93‑1 (MQ=255)

               cagGCGTAAACATACAGTTTCCACAAACTCCCTACCGGCGTGGGGAGCGAGTCAGGTAATGCTGGGAATTTGGTCGCGCCTGATGAAGACAGc                                                                          >  2:5031497/1‑93 (MQ=255)

               cagGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGc                                                                          >  1:2832504/1‑93 (MQ=255)

               cagGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGc                                                                          >  2:7542131/1‑93 (MQ=255)

               cagGCGTAAACATACAGTTTCCACAAACGCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAAACTTTGGTCGCGCCTGATGACGACAGc                                                                          <  1:868124/93‑1 (MQ=255)

                agGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCt                                                                         >  2:1980587/1‑93 (MQ=255)

                agGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCt                                                                         >  1:4377341/1‑93 (MQ=255)

                    gTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATa                                                                     >  2:9506613/1‑93 (MQ=255)

                     tAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTTATGACAACAGCTGGTAc                                                                    >  2:6315867/1‑93 (MQ=255)

                     tAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATAc                                                                    >  1:4317552/1‑93 (MQ=255)

                     tAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATAc                                                                    >  2:8918507/1‑93 (MQ=255)

                     tAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGATGATAc                                                                    >  2:7963364/1‑93 (MQ=255)

                      aaaCATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACa                                                                   >  2:3069699/1‑93 (MQ=255)

                      aaaCACACAGTTTCCACAAACTCTCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACa                                                                   <  2:6900728/93‑1 (MQ=255)

                       aaCATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAg                                                                  >  1:1030425/1‑93 (MQ=255)

                          aTACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTg                                                               >  1:3525865/1‑93 (MQ=255)

                          aTACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTg                                                               >  2:2131576/1‑93 (MQ=255)

                           tACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGa                                                              <  2:610557/93‑1 (MQ=255)

                            aCAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGAt                                                             >  2:5724607/1‑93 (MQ=255)

                            aCAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGAt                                                             <  1:8765049/93‑1 (MQ=255)

                            aCAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTAAt                                                             >  1:150506/1‑93 (MQ=255)

                            aCAGATTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGAt                                                             >  2:6490201/1‑93 (MQ=255)

                             cAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATc                                                            >  2:734003/1‑93 (MQ=255)

                             cAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATc                                                            >  2:9130073/1‑93 (MQ=255)

                              aGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCg                                                           <  2:2654592/93‑1 (MQ=255)

                               gTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGt                                                          <  2:8214575/93‑1 (MQ=255)

                               gTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGt                                                          <  1:1757277/93‑1 (MQ=255)

                                tttCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCggt                                                         >  2:3730221/1‑91 (MQ=255)

                                tttCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGtg                                                         >  2:5203812/1‑93 (MQ=255)

                                tttCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGtg                                                         <  2:2994118/93‑1 (MQ=255)

                                 ttCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGtgc                                                        <  1:1545659/93‑1 (MQ=255)

                                 ttCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGtgc                                                        <  1:9383728/93‑1 (MQ=255)

                                  tCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGtgct                                                       <  2:4986180/93‑1 (MQ=255)

                                  tCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGtgct                                                       >  1:7445706/1‑93 (MQ=255)

                                   ccACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGtgctg                                                      >  2:9772080/1‑93 (MQ=255)

                                   ccACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGtgctg                                                      <  1:5205075/93‑1 (MQ=255)

                                   ccACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGtgctg                                                      <  1:8304100/93‑1 (MQ=255)

                                        aaCTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCAc                                                 >  1:6328976/1‑93 (MQ=255)

                                        aaCTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCGGCGCAc                                                 >  1:1982713/1‑93 (MQ=255)

                                         aCTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCAcc                                                >  1:7028131/1‑93 (MQ=255)

                                          cTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACcg                                               <  2:4968627/93‑1 (MQ=255)

                                           tCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATGCAGTTGATCGTGCTGCGCACcgc                                              >  2:5533324/1‑93 (MQ=255)

                                           tCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTTCGCACcgc                                              >  1:3801182/1‑93 (MQ=255)

                                           tCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACcgc                                              >  2:9344262/1‑93 (MQ=255)

                                             ccTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACAgcgc                                            >  2:3103896/1‑93 (MQ=255)

                                              cTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCgca                                           >  2:1344974/1‑93 (MQ=255)

                                              cTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCgca                                           <  1:9980801/93‑1 (MQ=255)

                                              cTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCgca                                           >  1:9055741/1‑93 (MQ=255)

                                                aaCGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCgcagc                                         <  2:7983290/93‑1 (MQ=255)

                                                 aCGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCgcagca                                        >  1:7874975/1‑93 (MQ=255)

                                                 aCGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCgcagca                                        >  2:831338/1‑93 (MQ=255)

                                                      gtggtgAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGt                                   >  1:2047650/1‑93 (MQ=255)

                                                         gtgAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGt                                >  2:4096829/1‑93 (MQ=255)

                                                          tgAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTa                               >  1:8543866/1‑93 (MQ=255)

                                                          tgAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTa                               >  1:5997721/1‑93 (MQ=255)

                                                          ggAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTa                               <  2:6303519/92‑1 (MQ=255)

                                                          ggAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTa                               <  1:5376601/92‑1 (MQ=255)

                                                           gAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTaa                              <  2:2024103/93‑1 (MQ=255)

                                                           gAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGGaa                              >  2:8698422/1‑93 (MQ=255)

                                                            aGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAAc                             >  1:3494255/1‑93 (MQ=255)

                                                            aGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAAc                             <  2:7635249/93‑1 (MQ=255)

                                                            aGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAAc                             <  2:7388250/93‑1 (MQ=255)

                                                             gCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACg                            <  1:4180088/93‑1 (MQ=255)

                                                               gAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGc                          <  1:7748769/93‑1 (MQ=255)

                                                                 gTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGt                        <  1:6192295/93‑1 (MQ=255)

                                                                  tCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGtc                       >  1:6516102/1‑93 (MQ=255)

                                                                   cAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGtct                      <  2:4230821/93‑1 (MQ=255)

                                                                    aGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGtctc                     >  2:9630577/1‑93 (MQ=255)

                                                                     ggTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTctcc                    >  1:3198113/1‑93 (MQ=255)

                                                                     ggTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTctcc                    >  2:5650886/1‑93 (MQ=255)

                                                                      gTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTctcct                   >  2:6270768/1‑93 (MQ=255)

                                                                        aaTGCTGAGACTTTGGTCGGACCTGATGGCGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCtcctcc                 <  2:3403013/93‑1 (MQ=255)

                                                                         aTGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCg                >  2:7017626/1‑93 (MQ=255)

                                                                         aTGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCg                >  2:3624141/1‑93 (MQ=255)

                                                                              gagaCTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAgt           >  1:4565388/1‑93 (MQ=255)

                                                                              gagaCTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAgt           <  1:9938170/93‑1 (MQ=255)

                                                                                  cTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTg       <  1:295060/93‑1 (MQ=255)

                                                                                   tttGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTgg      >  2:3229321/1‑93 (MQ=255)

                                                                                   tttGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGCGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTgg      >  2:1538334/1‑93 (MQ=255)

                                                                                    ttGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGc     <  1:1826370/93‑1 (MQ=255)

                                                                                     tGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCa    <  1:2202295/93‑1 (MQ=255)

                                                                                     tGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGGCTCCTCCCCAAGTGTTGGCa    >  2:4304244/1‑93 (MQ=255)

                                                                                       gTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCagg  >  1:3919283/1‑92 (MQ=255)

                                                                                       gTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCaga  >  1:4120835/1‑93 (MQ=255)

                                                                                       gTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCaga  >  2:2471058/1‑93 (MQ=255)

TGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGA  >  NC_000913/2327597‑2327776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAA  >  NC_000913/2327594‑2327780

TGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCG                                                                                               >  SRR4302540.9011467/1‑94 (MQ=60)

 GATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGC                                                                                              >  SRR4302540.125822/1‑94 (MQ=60)

  ATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCG                                                                                             >  SRR4302540.2826396/1‑94 (MQ=60)

  ATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCG                                                                                             <  SRR4302540.10113475/94‑1 (MQ=60)

     GGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCT                                                                                          <  SRR4302540.231804/94‑1 (MQ=60)

     GGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCT                                                                                          >  SRR4302540.8821779/1‑94 (MQ=60)

      GTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTG                                                                                         >  SRR4302540.154672/1‑94 (MQ=60)

       TATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGA                                                                                        >  SRR4302540.9585656/1‑94 (MQ=60)

        ATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGAT                                                                                       >  SRR4302540.5057290/1‑94 (MQ=60)

        ATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGAT                                                                                       >  SRR4302540.4973116/1‑94 (MQ=60)

          CCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGA                                                                                     >  SRR4302540.8424275/1‑94 (MQ=60)

           CTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGAC                                                                                    >  SRR4302540.14064/1‑94 (MQ=60)

           CTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGAC                                                                                    <  SRR4302540.3543791/94‑1 (MQ=60)

           CTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGAC                                                                                    >  SRR4302540.4542594/1‑94 (MQ=60)

           CTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGAC                                                                                    <  SRR4302540.6701218/94‑1 (MQ=60)

           CTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGAC                                                                                    <  SRR4302540.181191/94‑1 (MQ=60)

            TCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACG                                                                                   >  SRR4302540.9346640/1‑94 (MQ=60)

             CCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGA                                                                                  >  SRR4302540.4107874/1‑94 (MQ=60)

              CAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGAC                                                                                 <  SRR4302540.2377659/94‑1 (MQ=60)

              CAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGAC                                                                                 <  SRR4302540.2851410/94‑1 (MQ=60)

              CAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGAC                                                                                 <  SRR4302540.7384098/94‑1 (MQ=60)

              CAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGAC                                                                                 <  SRR4302540.1913296/94‑1 (MQ=60)

              CAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGAC                                                                                 <  SRR4302540.1417994/94‑1 (MQ=60)

                GCCAGGCGTAAACATACAGTTTCCACAATCTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAG                                                                               <  SRR4302540.849510/94‑1 (MQ=60)

                 CCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGC                                                                              >  SRR4302540.3295573/1‑94 (MQ=60)

                 CCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGC                                                                              >  SRR4302540.2863138/1‑94 (MQ=60)

                  CAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCT                                                                             >  SRR4302540.4424872/1‑94 (MQ=60)

                  CAGGCGTAAACATACAGTTTCCACAAACGCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAAACTTTGGTCGCGCCTGATGACGACAGCT                                                                             <  SRR4302540.877334/94‑1 (MQ=60)

                      CGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATA                                                                         >  SRR4302540.6927893/1‑94 (MQ=60)

                       GTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATAC                                                                        >  SRR4302540.4364556/1‑94 (MQ=60)

                       GTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATAC                                                                        >  SRR4302540.5824736/1‑94 (MQ=60)

                        TAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACA                                                                       <  SRR4302540.4415842/94‑1 (MQ=60)

                         AAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAG                                                                      >  SRR4302540.1040887/1‑94 (MQ=60)

                         AAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAG                                                                      <  SRR4302540.8783998/94‑1 (MQ=60)

                          AACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGT                                                                     >  SRR4302540.1473034/1‑94 (MQ=60)

                          AACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGT                                                                     >  SRR4302540.4562598/1‑94 (MQ=60)

                          AACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGT                                                                     <  SRR4302540.2694617/94‑1 (MQ=60)

                          AACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGT                                                                     >  SRR4302540.3785538/1‑94 (MQ=60)

                            CATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTG                                                                   >  SRR4302540.10040312/1‑94 (MQ=60)

                            CATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTG                                                                   >  SRR4302540.3564318/1‑94 (MQ=60)

                            CATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTG                                                                   <  SRR4302540.4231038/94‑1 (MQ=60)

                            CATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTG                                                                   >  SRR4302540.9124348/1‑94 (MQ=60)

                            CATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTG                                                                   >  SRR4302540.8090950/1‑94 (MQ=60)

                            CATACAGTTTCCACAAACTCCCTAACGGCGTGGGGAGCGAGTCAGGTAATGGTGAGACTTTGGTGACGCCTGATGACGACAGATGATACAGTTA                                                                   >  SRR4302540.2960672/1‑94 (MQ=60)

                             ATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGA                                                                  >  SRR4302540.7906477/1‑94 (MQ=60)

                             ATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGA                                                                  >  SRR4302540.9384154/1‑94 (MQ=60)

                             ATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGA                                                                  >  SRR4302540.7906476/1‑94 (MQ=60)

                             ATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGA                                                                  >  SRR4302540.3956767/1‑94 (MQ=60)

                              TACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGAT                                                                 <  SRR4302540.4178770/94‑1 (MQ=60)

                              TACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTAAT                                                                 >  SRR4302540.152056/1‑94 (MQ=60)

                               ACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATC                                                                <  SRR4302540.8866658/94‑1 (MQ=60)

                                 AGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGT                                                              >  SRR4302540.3567263/1‑94 (MQ=60)

                                  ccgtcaacaatgaCCCTAAAGGCGTCGTGAGCGAGTCATGTAATGCTGACACTTTGGCCGCGCCTGATGACGACAGCTGATACAGTTGATCGTG                                                             <  SRR4302540.1776322/81‑1 (MQ=60)

                                  GTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTG                                                             >  SRR4302540.6309458/1‑94 (MQ=60)

                                  GTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTG                                                             <  SRR4302540.1776321/94‑1 (MQ=60)

                                   TTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGC                                                            <  SRR4302540.2016481/94‑1 (MQ=60)

                                    TTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCT                                                           <  SRR4302540.9495418/94‑1 (MQ=60)

                                    TTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCT                                                           <  SRR4302540.1562716/94‑1 (MQ=60)

                                    TTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCT                                                           >  SRR4302540.7528667/1‑94 (MQ=60)

                                      CCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGC                                                         <  SRR4302540.8400528/94‑1 (MQ=60)

                                      CCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGC                                                         <  SRR4302540.6265554/94‑1 (MQ=60)

                                      CCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGC                                                         <  SRR4302540.5261771/94‑1 (MQ=60)

                                       CACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCG                                                        >  SRR4302540.1986147/1‑94 (MQ=60)

                                          AAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCAC                                                     >  SRR4302540.6399451/1‑94 (MQ=60)

                                          AAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCGGCGCAC                                                     >  SRR4302540.2004211/1‑94 (MQ=60)

                                           AACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACC                                                    >  SRR4302540.7106220/1‑94 (MQ=60)

                                            ACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCG                                                   >  SRR4302540.4993787/1‑94 (MQ=60)

                                             CTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTTCGCACCGC                                                  >  SRR4302540.3842593/1‑94 (MQ=60)

                                              TCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTGGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCG                                                 <  SRR4302540.6693248/94‑1 (MQ=60)

                                              CCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCG                                                 <  SRR4302540.3485855/94‑1 (MQ=60)

                                                CCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCA                                               >  SRR4302540.9164457/1‑94 (MQ=60)

                                                 CTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAG                                              <  SRR4302540.10097182/94‑1 (MQ=60)

                                                   AACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCA                                            >  SRR4302540.7966960/1‑94 (MQ=60)

                                                    ACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCAC                                           <  SRR4302540.2920145/94‑1 (MQ=60)

                                                        CGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGT                                       >  SRR4302540.2069707/1‑94 (MQ=60)

                                                         GTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTT                                      >  SRR4302540.6681422/1‑94 (MQ=60)

                                                         GTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTT                                      >  SRR4302540.2144634/1‑94 (MQ=60)

                                                          TGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTG                                     <  SRR4302540.571161/94‑1 (MQ=60)

                                                            GTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTA                                   >  SRR4302540.6064187/1‑94 (MQ=60)

                                                            GTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTA                                   >  SRR4302540.8643133/1‑94 (MQ=60)

                                                             GGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAA                                  <  SRR4302540.5435728/94‑1 (MQ=60)

                                                              GAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAAC                                 >  SRR4302540.3532311/1‑94 (MQ=60)

                                                              GAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAAC                                 <  SRR4302540.5048953/94‑1 (MQ=60)

                                                               AGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACG                                <  SRR4302540.2779634/94‑1 (MQ=60)

                                                                GCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGG                               <  SRR4302540.4225487/94‑1 (MQ=60)

                                                                  GAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCG                             <  SRR4302540.7839244/94‑1 (MQ=60)

                                                                  GAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCG                             <  SRR4302540.5151995/94‑1 (MQ=60)

                                                                   AGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGT                            >  SRR4302540.435055/1‑94 (MQ=60)

                                                                   AGTCAGGTAATGCTGAGACTTTGGTCGCGACTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGT                            <  SRR4302540.216449/94‑1 (MQ=60)

                                                                    GTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTC                           >  SRR4302540.6588493/1‑94 (MQ=60)

                                                                    GTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTC                           <  SRR4302540.6261245/94‑1 (MQ=60)

                                                                    GTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTC                           >  SRR4302540.1668383/1‑94 (MQ=60)

                                                                    GTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTC                           >  SRR4302540.1668382/1‑94 (MQ=60)

                                                                    GTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTC                           >  SRR4302540.8717328/1‑94 (MQ=60)

                                                                       AGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCC                        >  SRR4302540.3232850/1‑94 (MQ=60)

                                                                         GTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTC                      <  SRR4302540.7574069/94‑1 (MQ=60)

                                                                          TAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCC                     >  SRR4302540.9707058/1‑94 (MQ=60)

                                                                           AATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCG                    >  SRR4302540.954059/1‑94 (MQ=60)

                                                                              GCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCCCCGCGCAGCACCCGTTGTAACGGCGTATCCTCCGCAA                 >  SRR4302540.4925536/1‑94 (MQ=60)

                                                                               CTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGGACCGCGCAGCGCCAGTTGTAAtcgcctcccctcctctcg                >  SRR4302540.5504345/1‑76 (MQ=60)

                                                                               CTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAG                <  SRR4302540.6255748/94‑1 (MQ=60)

                                                                                TGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGT               >  SRR4302540.122698/1‑94 (MQ=60)

                                                                                TGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGT               >  SRR4302540.4614687/1‑94 (MQ=60)

                                                                                 GAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTG              <  SRR4302540.10054159/94‑1 (MQ=60)

                                                                                 GAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTG              >  SRR4302540.7421454/1‑94 (MQ=60)

                                                                                 GAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTG              >  SRR4302540.5388236/1‑94 (MQ=60)

                                                                                  AGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGGCCACCGCCCAGCACCAGTTGTAACAGCGCCTCCTCCGCCAGTGT             >  SRR4302540.3964611/1‑94 (MQ=60)

                                                                                    ACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTG           >  SRR4302540.10095272/1‑94 (MQ=60)

                                                                                     CTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGG          <  SRR4302540.297974/94‑1 (MQ=60)

                                                                                     CTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGG          <  SRR4302540.5212492/94‑1 (MQ=60)

                                                                                       TTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCA        <  SRR4302540.1846342/94‑1 (MQ=60)

                                                                                        TGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAG       <  SRR4302540.2226085/94‑1 (MQ=60)

                                                                                        TGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAG       <  SRR4302540.6562380/94‑1 (MQ=60)

                                                                                         GGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGG      >  SRR4302540.3961709/1‑94 (MQ=60)

                                                                                         GGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGA      >  SRR4302540.4165329/1‑94 (MQ=60)

                                                                                          GTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCAGCAGTTGTCACGGCGTCTCCTCCGCAAGTGTTGGCAGCG     >  SRR4302540.7769283/1‑94 (MQ=60)

                                                                                          GTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAG     <  SRR4302540.3097080/94‑1 (MQ=60)

                                                                                          GTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGGCTCCTCCGCAAGTGTTGGCAGAG     >  SRR4302540.7769284/1‑94 (MQ=60)

                                                                                             GCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAA  <  SRR4302540.9613013/94‑1 (MQ=60)

                                                                                             GCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGGAA  >  SRR4302540.2246020/1‑94 (MQ=60)

TGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAA  >  NC_000913/2327594‑2327780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`