Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,327,687	A→G	V44A (GTG→GCG)	b2226 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,327,687	0	A	G	100.0%	779.6 / NA	242	V44A (GTG→GCG)	b2226	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (138/104); total (138/104)

Only 100 of 251 total aligned reads displayed.

TGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGA  >  NC_000913/2327597‑2327776

tGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTcgcg                                                                                         >  1:4086415/1‑93 (MQ=255)

tGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTcgcg                                                                                         >  2:3553958/1‑93 (MQ=255)

tGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTcgcg                                                                                         >  2:5695573/1‑93 (MQ=255)

  ggTATCCTCAAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGcc                                                                                       <  1:5125048/93‑1 (MQ=255)

     aTCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCtga                                                                                    <  2:4882759/93‑1 (MQ=255)

      tcctccAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCtgat                                                                                   >  2:3901182/1‑93 (MQ=255)

      tcctccAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCtgat                                                                                   <  2:5513690/93‑1 (MQ=255)

       cctccAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCtgatg                                                                                  <  1:5848371/93‑1 (MQ=255)

         tccAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATgac                                                                                <  2:1124329/93‑1 (MQ=255)

         tccAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATgac                                                                                >  2:2840827/1‑93 (MQ=255)

         tccAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATgac                                                                                >  1:2547415/1‑93 (MQ=255)

         tccAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATgac                                                                                <  2:5988684/93‑1 (MQ=255)

          ccagccagGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATgacg                                                                               >  1:2009926/1‑93 (MQ=255)

           cagccagGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATgacga                                                                              <  1:4620737/93‑1 (MQ=255)

           cagccagGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATgacga                                                                              <  2:3058963/93‑1 (MQ=255)

           cagccagGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATgacga                                                                              >  1:4324285/1‑93 (MQ=255)

            agccagGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATgacgac                                                                             <  2:4456577/93‑1 (MQ=255)

             gccagGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACa                                                                            <  1:6030868/93‑1 (MQ=255)

             gccagGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACa                                                                            >  1:2048794/1‑93 (MQ=255)

               cagGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGc                                                                          <  1:2586268/93‑1 (MQ=255)

               cagGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGc                                                                          <  1:996842/93‑1 (MQ=255)

               cagGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGc                                                                          <  1:3771709/93‑1 (MQ=255)

               cagGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGc                                                                          >  1:6173908/1‑93 (MQ=255)

                cggCGTAAACATACAGTTTCCACAACCTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCt                                                                         <  1:4304008/92‑1 (MQ=255)

                agGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCt                                                                         <  1:3695550/93‑1 (MQ=255)

                 ggCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTg                                                                        >  1:1305512/1‑93 (MQ=255)

                  gCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGa                                                                       <  1:4209769/93‑1 (MQ=255)

                  gCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGa                                                                       <  1:3282783/93‑1 (MQ=255)

                    gTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATa                                                                     >  1:4020794/1‑93 (MQ=255)

                    gTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATa                                                                     >  1:2336987/1‑93 (MQ=255)

                     tAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATAc                                                                    <  2:4086146/93‑1 (MQ=255)

                      aaaCATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACa                                                                   <  2:2523837/93‑1 (MQ=255)

                      aaaCATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACa                                                                   >  1:5952981/1‑93 (MQ=255)

                       aaCATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAg                                                                  >  2:4598341/1‑93 (MQ=255)

                        aCATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGt                                                                 >  2:779842/1‑93 (MQ=255)

                         cATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGtt                                                                >  2:4342829/1‑93 (MQ=255)

                          aTACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTg                                                               >  1:10495/1‑93 (MQ=255)

                           tACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGa                                                              <  2:4940827/93‑1 (MQ=255)

                           tACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGa                                                              <  1:2470682/93‑1 (MQ=255)

                           tACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGa                                                              >  1:1933524/1‑93 (MQ=255)

                            aCAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGAt                                                             >  2:1388835/1‑93 (MQ=255)

                            aCAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGAt                                                             <  2:5060981/93‑1 (MQ=255)

                             cAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATc                                                            <  2:4869691/93‑1 (MQ=255)

                              aGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCg                                                           <  1:1751083/93‑1 (MQ=255)

                                 ttCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGtgc                                                        <  1:1059657/93‑1 (MQ=255)

                                  tCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGtgct                                                       <  1:58820/93‑1 (MQ=255)

                                    cacaATCTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGtgctgc                                                     <  2:2461044/93‑1 (MQ=255)

                                    cacaAACTCCCGAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGtgctgc                                                     <  1:5373068/93‑1 (MQ=255)

                                     acaAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTgcg                                                    >  1:843292/1‑93 (MQ=255)

                                        aaCTCCCTAACGGCGTGTTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCAc                                                 <  2:6299264/93‑1 (MQ=255)

                                        aaCTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCAc                                                 >  2:1384346/1‑93 (MQ=255)

                                         aCTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCAcc                                                <  1:4934377/93‑1 (MQ=255)

                                         aCTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCAcc                                                >  2:2120219/1‑93 (MQ=255)

                                          cTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACcg                                               <  1:2540110/93‑1 (MQ=255)

                                          cTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACcg                                               >  2:286374/1‑93 (MQ=255)

                                          cTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACcg                                               >  2:4038965/1‑93 (MQ=255)

                                           tCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACcgc                                              >  1:4944940/1‑93 (MQ=255)

                                           tCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACcgc                                              >  2:6260862/1‑93 (MQ=255)

                                              cTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCgca                                           >  1:3475296/1‑93 (MQ=255)

                                              cTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCgca                                           >  1:2683451/1‑93 (MQ=255)

                                               tAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCgcgcgg                                          >  2:2939030/1‑91 (MQ=255)

                                               tAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCgcag                                          >  2:1809410/1‑93 (MQ=255)

                                                 aCGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCgcagcc                                        >  1:3396758/1‑92 (MQ=255)

                                                  cGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCAc                                       <  2:1346628/93‑1 (MQ=255)

                                                    gCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCa                                     >  2:3160697/1‑93 (MQ=255)

                                                    gCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCa                                     >  2:227078/1‑93 (MQ=255)

                                                       tggtgAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGtt                                  >  2:4193151/1‑93 (MQ=255)

                                                            aGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTaaa                             >  2:228670/1‑92 (MQ=255)

                                                            aGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAAc                             <  2:6264705/93‑1 (MQ=255)

                                                              cGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACgg                           <  1:1465787/93‑1 (MQ=255)

                                                               gAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACggg                          >  2:5820770/1‑92 (MQ=255)

                                                                 gTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGt                        >  1:3098648/1‑93 (MQ=255)

                                                                  tCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGtc                       >  2:3384012/1‑93 (MQ=255)

                                                                  tCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGtc                       >  1:3203907/1‑93 (MQ=255)

                                                                  tCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGtc                       <  1:702513/93‑1 (MQ=255)

                                                                   cAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGGTGTAACGGCGtct                      >  2:1244448/1‑93 (MQ=255)

                                                                    aGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGtctc                     >  2:47481/1‑93 (MQ=255)

                                                                     ggTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTctcc                    >  1:2150714/1‑93 (MQ=255)

                                                                     ggTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTctcc                    >  2:1986224/1‑93 (MQ=255)

                                                                     ggTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTctcc                    >  2:3122072/1‑93 (MQ=255)

                                                                       tAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTctcctc                  >  1:665885/1‑93 (MQ=255)

                                                                       tAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTctcctc                  <  2:649150/93‑1 (MQ=255)

                                                                        aaTGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCtcctcc                 >  2:2073749/1‑93 (MQ=255)

                                                                        aaTGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCtcctcc                 <  1:1847141/93‑1 (MQ=255)

                                                                          tGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGc               >  2:6022783/1‑93 (MQ=255)

                                                                            cTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCaa             >  2:1595244/1‑93 (MQ=255)

                                                                             tGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAg            <  1:4696033/93‑1 (MQ=255)

                                                                              gagaCTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAgt           <  1:3821023/93‑1 (MQ=255)

                                                                                gaCTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAgtgt         <  1:5199398/93‑1 (MQ=255)

                                                                                gaCTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAgtgt         <  1:5093433/93‑1 (MQ=255)

                                                                                 aCTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGtt        >  1:2785425/1‑93 (MQ=255)

                                                                                 aCTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGtt        <  1:3997206/93‑1 (MQ=255)

                                                                                  cTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTg       <  2:3600665/93‑1 (MQ=255)

                                                                                   tttGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTgg      >  2:5015838/1‑93 (MQ=255)

                                                                                   tttGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTgg      >  1:766861/1‑93 (MQ=255)

                                                                                   tttGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTgg      <  2:4146690/93‑1 (MQ=255)

                                                                                     tGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCa    <  1:265792/93‑1 (MQ=255)

                                                                                     tGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCa    >  1:3033822/1‑93 (MQ=255)

                                                                                       gTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCaga  >  2:158018/1‑93 (MQ=255)

                                                                                       gTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCaga  >  1:4477934/1‑93 (MQ=255)

TGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGA  >  NC_000913/2327597‑2327776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAA  >  NC_000913/2327594‑2327780

TGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCG                                                                                               <  SRR4302533.4015340/94‑1 (MQ=60)

TGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCG                                                                                               <  SRR4302533.1671754/94‑1 (MQ=60)

 GATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGC                                                                                              >  SRR4302533.2161283/1‑94 (MQ=60)

 GATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGC                                                                                              >  SRR4302533.3588793/1‑94 (MQ=60)

  ATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCG                                                                                             >  SRR4302533.4111293/1‑94 (MQ=60)

   TGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGC                                                                                            >  SRR4302533.4920198/1‑94 (MQ=60)

     GGTATCCTCAAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCT                                                                                          <  SRR4302533.5156008/94‑1 (MQ=60)

       TATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGA                                                                                        >  SRR4302533.844046/1‑94 (MQ=60)

        ATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGAT                                                                                       >  SRR4302533.5908265/1‑94 (MQ=60)

        ATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGAT                                                                                       >  SRR4302533.4902793/1‑94 (MQ=60)

        ATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGAT                                                                                       <  SRR4302533.2590325/94‑1 (MQ=60)

         TCCTCCAGCCATGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATG                                                                                      <  SRR4302533.2193406/94‑1 (MQ=60)

          CCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGA                                                                                     <  SRR4302533.5883725/94‑1 (MQ=60)

           CTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGAC                                                                                    >  SRR4302533.5219999/1‑94 (MQ=60)

           CTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGAC                                                                                    <  SRR4302533.4690330/94‑1 (MQ=60)

           CTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGAC                                                                                    >  SRR4302533.2563093/1‑94 (MQ=60)

            TCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACG                                                                                   >  SRR4302533.2022307/1‑94 (MQ=60)

             CCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGA                                                                                  >  SRR4302533.4350326/1‑94 (MQ=60)

             CCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGA                                                                                  <  SRR4302533.6276747/94‑1 (MQ=60)

             CCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGA                                                                                  <  SRR4302533.4344927/94‑1 (MQ=60)

              CAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGAC                                                                                 <  SRR4302533.4648742/94‑1 (MQ=60)

               AGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACA                                                                                >  SRR4302533.2061428/1‑94 (MQ=60)

                GCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAG                                                                               <  SRR4302533.6067417/94‑1 (MQ=60)

                 CCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGC                                                                              <  SRR4302533.4038296/94‑1 (MQ=60)

                 CCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGC                                                                              >  SRR4302533.1058970/1‑94 (MQ=60)

                 CCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGC                                                                              >  SRR4302533.6211011/1‑94 (MQ=60)

                  CAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCT                                                                             <  SRR4302533.1002663/94‑1 (MQ=60)

                  CAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCT                                                                             <  SRR4302533.2602168/94‑1 (MQ=60)

                  CAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCT                                                                             <  SRR4302533.3794830/94‑1 (MQ=60)

                  CAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCT                                                                             <  SRR4302533.3196705/94‑1 (MQ=60)

                  CAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCT                                                                             >  SRR4302533.4036005/1‑94 (MQ=60)

                   CGGCGTAAACATACAGTTTCCACAACCTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTG                                                                            <  SRR4302533.4329903/94‑1 (MQ=60)

                   AGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTG                                                                            <  SRR4302533.2508857/94‑1 (MQ=60)

                   AGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTG                                                                            <  SRR4302533.3718250/94‑1 (MQ=60)

                   AGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTG                                                                            >  SRR4302533.3375075/1‑94 (MQ=60)

                   AGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTG                                                                            >  SRR4302533.1313357/1‑94 (MQ=60)

                     GCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGAT                                                                          <  SRR4302533.3303048/94‑1 (MQ=60)

                     GCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGAT                                                                          <  SRR4302533.4235235/94‑1 (MQ=60)

                      CGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGCTA                                                                         >  SRR4302533.1896382/1‑94 (MQ=60)

                      CGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATA                                                                         >  SRR4302533.2351452/1‑94 (MQ=60)

                      CGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATA                                                                         >  SRR4302533.4045276/1‑94 (MQ=60)

                      CGTAAACATACAGTTCCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATA                                                                         <  SRR4302533.5877217/94‑1 (MQ=60)

                       GTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATAC                                                                        <  SRR4302533.4209148/94‑1 (MQ=60)

                       GTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATAC                                                                        >  SRR4302533.121398/1‑94 (MQ=60)

                        TAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACA                                                                       >  SRR4302533.5988991/1‑94 (MQ=60)

                          AACATACAGTTTCCACAACCTCCCT‑ACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTT                                                                    <  SRR4302533.1855743/94‑1 (MQ=60)

                          AACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGT                                                                     <  SRR4302533.898828/94‑1 (MQ=60)

                          AACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGT                                                                     >  SRR4302533.702256/1‑94 (MQ=60)

                          AACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGT                                                                     >  SRR4302533.2165219/1‑94 (MQ=60)

                            CATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTG                                                                   >  SRR4302533.4974818/1‑94 (MQ=60)

                            CATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTG                                                                   >  SRR4302533.10581/1‑94 (MQ=60)

                             ATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGA                                                                  >  SRR4302533.4248466/1‑94 (MQ=60)

                             ATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGA                                                                  >  SRR4302533.1945322/1‑94 (MQ=60)

                             ATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGA                                                                  >  SRR4302533.6130539/1‑94 (MQ=60)

                              TACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGATACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGAT                                                                 <  SRR4302533.203387/94‑1 (MQ=60)

                              TACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGAT                                                                 <  SRR4302533.2485921/94‑1 (MQ=60)

                               ACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATC                                                                <  SRR4302533.3521432/94‑1 (MQ=60)

                                 AGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGT                                                              >  SRR4302533.5130665/1‑94 (MQ=60)

                                 AGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGT                                                              <  SRR4302533.1761745/94‑1 (MQ=60)

                                  GTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTG                                                             >  SRR4302533.3089938/1‑94 (MQ=60)

                                    TTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCT                                                           <  SRR4302533.5449752/94‑1 (MQ=60)

                                    TTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCT                                                           <  SRR4302533.1065887/94‑1 (MQ=60)

                                     TCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTG                                                          <  SRR4302533.2755734/94‑1 (MQ=60)

                                     TCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTG                                                          <  SRR4302533.59207/94‑1 (MQ=60)

                                     TCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTG                                                          >  SRR4302533.3321147/1‑94 (MQ=60)

                                      CCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGC                                                         >  SRR4302533.1539294/1‑94 (MQ=60)

                                       CACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCG                                                        >  SRR4302533.848223/1‑94 (MQ=60)

                                       CACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCG                                                        <  SRR4302533.2543773/94‑1 (MQ=60)

                                       CACAAACTCCCGAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCG                                                        <  SRR4302533.5405351/94‑1 (MQ=60)

                                        ACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGC                                                       >  SRR4302533.4335558/1‑94 (MQ=60)

                                         CAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCA                                                      >  SRR4302533.391773/1‑94 (MQ=60)

                                         CAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCA                                                      >  SRR4302533.1921622/1‑94 (MQ=60)

                                          AAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCAC                                                     <  SRR4302533.766196/94‑1 (MQ=60)

                                          AAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTAGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCAC                                                     <  SRR4302533.1523367/94‑1 (MQ=60)

                                           AACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACC                                                    >  SRR4302533.2051894/1‑94 (MQ=60)

                                           AACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACC                                                    >  SRR4302533.3833814/1‑94 (MQ=60)

                                            ACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCG                                                   <  SRR4302533.4964256/94‑1 (MQ=60)

                                            ACTCCCTAACGGCGGGGTGAGCGAGGCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTtgctgacc                                                   >  SRR4302533.5331902/1‑86 (MQ=60)

                                             CTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGC                                                  <  SRR4302533.2555747/94‑1 (MQ=60)

                                             CTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGC                                                  <  SRR4302533.5986419/94‑1 (MQ=60)

                                             CTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGC                                                  >  SRR4302533.4974868/1‑94 (MQ=60)

                                              TCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCG                                                 >  SRR4302533.700146/1‑94 (MQ=60)

                                                CCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCA                                               >  SRR4302533.959555/1‑94 (MQ=60)

                                                CCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCA                                               >  SRR4302533.2699895/1‑94 (MQ=60)

                                                CCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCA                                               >  SRR4302533.3496739/1‑94 (MQ=60)

                                                   AACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCC                                            >  SRR4302533.2893523/1‑94 (MQ=60)

                                                   AACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCC                                            >  SRR4302533.3417733/1‑94 (MQ=60)

                                                      GGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCA                                         >  SRR4302533.3812584/1‑94 (MQ=60)

                                                      GGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCA                                         >  SRR4302533.5085741/1‑94 (MQ=60)

                                                         GTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTT                                      >  SRR4302533.262233/1‑94 (MQ=60)

                                                          TGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTG                                     >  SRR4302533.446532/1‑94 (MQ=60)

                                                          TGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTG                                     <  SRR4302533.5064417/94‑1 (MQ=60)

                                                            GTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTA                                   <  SRR4302533.2959103/94‑1 (MQ=60)

                                                            GTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTA                                   >  SRR4302533.3441281/1‑94 (MQ=60)

                                                            GTGAGCGAGTCAGGTAATGCTGACACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTA                                   >  SRR4302533.1460609/1‑94 (MQ=60)

                                                             TGAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAA                                  <  SRR4302533.269371/94‑1 (MQ=60)

                                                              GAGCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAAC                                 <  SRR4302533.405193/94‑1 (MQ=60)

                                                               AGTGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACG                                <  SRR4302533.5385575/94‑1 (MQ=60)

                                                                GCGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGG                               >  SRR4302533.4226096/1‑94 (MQ=60)

                                                                 CGAGTCAGTTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGC                              <  SRR4302533.3570339/94‑1 (MQ=60)

                                                                 CGAGTCAGGTAATGCTGAGACTTTGGTCGCGCTTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGC                              <  SRR4302533.4556749/94‑1 (MQ=60)

                                                                 CGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGC                              >  SRR4302533.1234027/1‑94 (MQ=60)

                                                                 CGAGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGC                              <  SRR4302533.1474628/94‑1 (MQ=60)

                                                                   AGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGT                            >  SRR4302533.2692037/1‑94 (MQ=60)

                                                                   AGTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGT                            >  SRR4302533.3117698/1‑94 (MQ=60)

                                                                    GTCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTC                           >  SRR4302533.3223627/1‑94 (MQ=60)

                                                                     TCAGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCT                          <  SRR4302533.706668/94‑1 (MQ=60)

                                                                       AGGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCC                        >  SRR4302533.2163958/1‑94 (MQ=60)

                                                                        GGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCT                       <  SRR4302533.784437/94‑1 (MQ=60)

                                                                        GGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCT                       <  SRR4302533.2130811/94‑1 (MQ=60)

                                                                        GGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCT                       >  SRR4302533.6063477/1‑94 (MQ=60)

                                                                        GGTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCCTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCT                       <  SRR4302533.3152391/94‑1 (MQ=60)

                                                                         GTAATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTC                      >  SRR4302533.669819/1‑94 (MQ=60)

                                                                           AATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCG                    <  SRR4302533.1858401/94‑1 (MQ=60)

                                                                            ATGCTGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGC                   >  SRR4302533.5163304/1‑94 (MQ=60)

                                                                                TGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGT               <  SRR4302533.1958887/94‑1 (MQ=60)

                                                                                TGAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGT               <  SRR4302533.4724497/94‑1 (MQ=60)

                                                                                 GAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTG              <  SRR4302533.3228988/94‑1 (MQ=60)

                                                                                 GAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTG              <  SRR4302533.2561830/94‑1 (MQ=60)

                                                                                 GAGACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTG              <  SRR4302533.3844387/94‑1 (MQ=60)

                                                                                  AGACGTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGT             <  SRR4302533.2469282/94‑1 (MQ=60)

                                                                                   GACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTT            <  SRR4302533.5230746/94‑1 (MQ=60)

                                                                                   GACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTT            >  SRR4302533.2802540/1‑94 (MQ=60)

                                                                                   GACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTT            <  SRR4302533.5124202/94‑1 (MQ=60)

                                                                                    ACTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTG           <  SRR4302533.4021546/94‑1 (MQ=60)

                                                                                     CTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGG          >  SRR4302533.5920595/1‑94 (MQ=60)

                                                                                     CTTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGG          >  SRR4302533.771385/1‑94 (MQ=60)

                                                                                      TTTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGC         <  SRR4302533.6111332/94‑1 (MQ=60)

                                                                                       TTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCA        >  SRR4302533.2567409/1‑94 (MQ=60)

                                                                                       TTGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCA        >  SRR4302533.3052385/1‑94 (MQ=60)

                                                                                        TGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAG       <  SRR4302533.267411/94‑1 (MQ=60)

                                                                                        TGGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAG       <  SRR4302533.3742226/94‑1 (MQ=60)

                                                                                         GGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGA      >  SRR4302533.4612617/1‑94 (MQ=60)

                                                                                         GGTCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGA      >  SRR4302533.4505029/1‑94 (MQ=60)

                                                                                           TCGCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGT    <  SRR4302533.5026968/94‑1 (MQ=60)

                                                                                             GCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGGACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAA  >  SRR4302533.2003873/1‑94 (MQ=60)

                                                                                             GCGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAA  <  SRR4302533.4360340/94‑1 (MQ=60)

TGATGGGTATCCTCCAGCCAGGCGTAAACATACAGTTTCCACAAACTCCCTAACGGCGTGGTGAGCGAGTCAGGTAATGCTGAGACTTTGGTCACGCCTGATGACGACAGCTGATACAGTTGATCGTGCTGCGCACCGCGCAGCACCAGTTGTAACGGCGTCTCCTCCGCAAGTGTTGGCAGAGTAA  >  NC_000913/2327594‑2327780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`