Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,532,993 A→G D303G (GAC→GGC)  ptsI → PEP‑protein phosphotransferase system enzyme I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,532,9930AG99.7% 1023.6 / ‑5.4 321D303G (GAC→GGC) ptsIPEP‑protein phosphotransferase system enzyme I
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (149/171);  total (149/172)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

Only 100 of 329 total aligned reads displayed.

CTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGACCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGTA  >  NC_000913/2532903‑2533082
                                                                                          |                                                                                         
cTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGcc                                                                                         <  1:5056752/93‑1 (MQ=255)
cTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGcc                                                                                         >  1:3478492/1‑93 (MQ=255)
 tAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCcg                                                                                        >  1:4706393/1‑93 (MQ=255)
 tAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCcg                                                                                        >  1:7219739/1‑93 (MQ=255)
 tAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCcg                                                                                        <  1:2208430/93‑1 (MQ=255)
 tAACATTGGTACAGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCcg                                                                                        <  1:6028613/93‑1 (MQ=255)
  aaCATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCcgc                                                                                       <  2:1564305/93‑1 (MQ=255)
  aaCATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCcgc                                                                                       >  1:7348869/1‑93 (MQ=255)
    cATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGa                                                                                     <  2:4671256/93‑1 (MQ=255)
     aTTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGAc                                                                                    <  1:3843558/93‑1 (MQ=255)
      ttGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACg                                                                                   >  2:4192547/1‑93 (MQ=255)
        ggTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCa                                                                                 <  2:1847806/93‑1 (MQ=255)
        ggTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCa                                                                                 >  2:8062833/1‑93 (MQ=255)
        ggTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCa                                                                                 <  2:6955198/93‑1 (MQ=255)
         gTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCAc                                                                                <  2:8007884/93‑1 (MQ=255)
          tACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACt                                                                               <  1:8925543/93‑1 (MQ=255)
          tACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACt                                                                               >  1:271677/1‑93 (MQ=255)
           aCGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTg                                                                              >  1:6149172/1‑93 (MQ=255)
            cGGTTCGTGACGTTGGAGGTGCAGAGCGTAACGACGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGc                                                                             <  1:8982993/93‑1 (MQ=255)
            cGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGc                                                                             >  1:6290614/1‑93 (MQ=255)
            cGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGc                                                                             >  1:4173936/1‑93 (MQ=255)
             ggTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGcc                                                                            <  2:1405552/93‑1 (MQ=255)
              gTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGccc                                                                           >  1:4319893/1‑93 (MQ=255)
              gTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGccc                                                                           <  2:3380964/93‑1 (MQ=255)
               ttCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCa                                                                          <  1:8757817/93‑1 (MQ=255)
               ttCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCa                                                                          >  2:815546/1‑93 (MQ=255)
               ttCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCa                                                                          <  2:3017294/93‑1 (MQ=255)
               ttCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCa                                                                          <  2:6150687/93‑1 (MQ=255)
                  gTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTg                                                                       >  2:8849948/1‑93 (MQ=255)
                  ctGACGTTGAAGGTGCAGAGCGTAACGGCTCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTg                                                                       <  1:5330413/92‑1 (MQ=255)
                   tGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTga                                                                      >  2:2410629/1‑93 (MQ=255)
                   tGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTga                                                                      >  2:9372741/1‑93 (MQ=255)
                   tGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCCGTTCATGGGCCGCGACGCACTGCCCACTga                                                                      >  1:8215121/1‑93 (MQ=255)
                     aCGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgcag                                                                    >  1:1024420/1‑93 (MQ=255)
                     aCGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaag                                                                    <  2:1395382/93‑1 (MQ=255)
                        ttGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaagaag                                                                 >  2:1867059/1‑93 (MQ=255)
                         tGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaagaaga                                                                >  2:8638354/1‑93 (MQ=255)
                          gAAGGTGCAGAGCGTAACGGCTCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTGCATGGGCCGCGACGCACTGCCCACTgaagaagaa                                                               <  1:3527282/93‑1 (MQ=255)
                           aaGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAAc                                                              <  2:2838208/93‑1 (MQ=255)
                             ggTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAg                                                            >  1:7818826/1‑93 (MQ=255)
                              gTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGt                                                           <  2:7522506/93‑1 (MQ=255)
                              gTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGt                                                           >  1:8152384/1‑93 (MQ=255)
                               tGCAGAGCGTAACGGCGCTTAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGtt                                                          <  2:2603986/93‑1 (MQ=255)
                               tGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGtt                                                          <  1:9971445/93‑1 (MQ=255)
                                gCAGAGCGTAACGGCTCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGttt                                                         <  2:3916649/93‑1 (MQ=255)
                                gCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGttt                                                         >  2:4524499/1‑93 (MQ=255)
                                gCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGttt                                                         <  2:6881967/93‑1 (MQ=255)
                                gCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGttt                                                         <  2:6456563/93‑1 (MQ=255)
                                   gagCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTtgct                                                      >  1:5516187/1‑93 (MQ=255)
                                   gagCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTtgct                                                      >  1:7665837/1‑93 (MQ=255)
                                   gagCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTtgct                                                      <  1:6118155/93‑1 (MQ=255)
                                   gagCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGGACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTGtgct                                                      >  2:480164/1‑93 (MQ=255)
                                      cGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTgctgct                                                   >  2:9653740/1‑93 (MQ=255)
                                           cGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACaa                                              >  2:1307521/1‑93 (MQ=255)
                                           cGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACaa                                              <  1:4195837/93‑1 (MQ=255)
                                             gcgcTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAg                                            >  2:7295983/1‑93 (MQ=255)
                                             gcgcTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAg                                            <  1:9056309/93‑1 (MQ=255)
                                               gcTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCa                                          >  1:2919716/1‑93 (MQ=255)
                                               gcTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCa                                          <  1:7158882/93‑1 (MQ=255)
                                                 tGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGt                                        <  1:8561581/93‑1 (MQ=255)
                                                 tGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGt                                        <  2:7573752/93‑1 (MQ=255)
                                                  gAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTg                                       <  2:5608551/93‑1 (MQ=255)
                                                  gAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTg                                       <  1:5868939/93‑1 (MQ=255)
                                                  gAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTg                                       <  2:9546438/93‑1 (MQ=255)
                                                   aaGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTgg                                      <  1:1290360/93‑1 (MQ=255)
                                                   aaGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTgg                                      >  1:1053284/1‑93 (MQ=255)
                                                    aGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGc                                     >  1:9523668/1‑93 (MQ=255)
                                                    aGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGc                                     >  1:7549960/1‑93 (MQ=255)
                                                    aGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGc                                     <  1:6421410/93‑1 (MQ=255)
                                                    aGGCGTTGGTCTGTATCGTACTGAGTTCCTGGTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGATTGCTGCTTACAAAGCAGTGGc                                     >  2:7036157/1‑93 (MQ=255)
                                                      gCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTg                                   >  2:5321804/1‑93 (MQ=255)
                                                       cGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGa                                  <  2:1087445/93‑1 (MQ=255)
                                                         ttGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGGCGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAg                                <  2:1008767/93‑1 (MQ=255)
                                                          tGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGc                               <  1:9378783/93‑1 (MQ=255)
                                                            gTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCgt                             >  2:2067721/1‑93 (MQ=255)
                                                             tCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCgtg                            >  2:3978163/1‑93 (MQ=255)
                                                              cTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCgtgt                           <  1:9763671/93‑1 (MQ=255)
                                                              cTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCgtgt                           <  1:9175362/93‑1 (MQ=255)
                                                               tGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGtgtg                          <  1:9496016/93‑1 (MQ=255)
                                                                 tATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGc                        <  2:526891/93‑1 (MQ=255)
                                                                  aTCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCt                       <  2:9749283/93‑1 (MQ=255)
                                                                  aTCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCt                       >  1:1735799/1‑93 (MQ=255)
                                                                     gTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGc                    >  2:5866490/1‑93 (MQ=255)
                                                                     gTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGc                    >  1:5712744/1‑93 (MQ=255)
                                                                      tACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACACAGCAGTGGCTGAAGCGTGTGGCTCTCa                   >  1:4755657/1‑93 (MQ=255)
                                                                       aCTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCaa                  <  1:4550285/93‑1 (MQ=255)
                                                                       aCTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCAACTGAAGAAGAACAGATTGCTGCTTGCAAAGAAGTGGCTGAAGAGTGTGGCTCGcta                  >  1:1608926/1‑91 (MQ=255)
                                                                        cTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAg                 <  2:6588433/93‑1 (MQ=255)
                                                                        cTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAg                 >  1:1792366/1‑93 (MQ=255)
                                                                         tGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGc                <  2:5043873/93‑1 (MQ=255)
                                                                         tGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGc                >  1:6632911/1‑93 (MQ=255)
                                                                         tGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGc                <  2:6235231/93‑1 (MQ=255)
                                                                         tGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGc                <  1:4085267/93‑1 (MQ=255)
                                                                           aGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCgg              >  1:5967176/1‑93 (MQ=255)
                                                                           aGTGCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCgg              <  1:3207157/93‑1 (MQ=255)
                                                                              tCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTa           <  1:2526300/93‑1 (MQ=255)
                                                                               ccTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAt          <  2:5692000/93‑1 (MQ=255)
                                                                                 tGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcg        >  2:8251162/1‑93 (MQ=255)
                                                                                    tCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcgttc     <  2:1221382/93‑1 (MQ=255)
                                                                                       tGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGTa  <  2:5956122/93‑1 (MQ=255)
                                                                                          |                                                                                         
CTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGACCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGTA  >  NC_000913/2532903‑2533082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGACCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGTACCA  >  NC_000913/2532900‑2533085
                                                                                             |                                                                                            
acgctaacatgaacacggttagtaACGTTGAAGGTGCAGAGCGTCACCGCACTGACGGCGTTGGCCTCTATCGTACTGAGTTCCTGTTCATGGG                                                                                              <  SRR4302540.6942469/70‑1 (MQ=41)
 CGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGC                                                                                             >  SRR4302540.6476016/1‑94 (MQ=60)
 CGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGC                                                                                             >  SRR4302540.5865329/1‑94 (MQ=60)
  GCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCC                                                                                            <  SRR4302540.4218586/94‑1 (MQ=60)
  GCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCC                                                                                            >  SRR4302540.3516364/1‑94 (MQ=60)
   CTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCG                                                                                           >  SRR4302540.9833340/1‑94 (MQ=60)
   CTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCG                                                                                           >  SRR4302540.7300209/1‑94 (MQ=60)
   CTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCG                                                                                           >  SRR4302540.7300208/1‑94 (MQ=60)
   CTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCG                                                                                           <  SRR4302540.5111421/94‑1 (MQ=60)
   CTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCG                                                                                           >  SRR4302540.4756682/1‑94 (MQ=60)
    TAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGC                                                                                          >  SRR4302540.7430859/1‑94 (MQ=60)
    TAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGC                                                                                          <  SRR4302540.2232282/94‑1 (MQ=60)
    TAACATTGGTACAGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGC                                                                                          <  SRR4302540.6095508/94‑1 (MQ=60)
     AACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCG                                                                                         >  SRR4302540.7386173/1‑94 (MQ=60)
      ACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGA                                                                                        <  SRR4302540.7788170/94‑1 (MQ=60)
        ATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACG                                                                                      >  SRR4302540.1754990/1‑94 (MQ=60)
        ATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACG                                                                                      <  SRR4302540.3885357/94‑1 (MQ=60)
         TTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGC                                                                                     >  SRR4302540.8267579/1‑94 (MQ=60)
          TGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCA                                                                                    >  SRR4302540.7856189/1‑94 (MQ=60)
          TGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCA                                                                                    <  SRR4302540.7527501/94‑1 (MQ=60)
           GGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGAAC                                                                                   >  SRR4302540.3212670/1‑94 (MQ=60)
            GTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACT                                                                                  <  SRR4302540.918163/94‑1 (MQ=60)
            GTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACT                                                                                  >  SRR4302540.274352/1‑94 (MQ=60)
            GTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACT                                                                                  >  SRR4302540.4375217/1‑94 (MQ=60)
            GTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACT                                                                                  <  SRR4302540.4359939/94‑1 (MQ=60)
             TACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGTCGCTGAAGGCGTTGGTCTGTATCTTACTGAGTTCCTGTTCATGGGCCGCGACGCACTG                                                                                 <  SRR4302540.9145661/94‑1 (MQ=60)
             TACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTG                                                                                 >  SRR4302540.6217659/1‑94 (MQ=60)
             TACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTG                                                                                 <  SRR4302540.9029322/94‑1 (MQ=60)
              ACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGC                                                                                >  SRR4302540.6360642/1‑94 (MQ=60)
              ACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGC                                                                                >  SRR4302540.4219224/1‑94 (MQ=60)
               CGGTTCGTGACGTTGGAGGTGCAGAGCGTAACGACGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCC                                                                               <  SRR4302540.9090155/94‑1 (MQ=60)
               CGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCC                                                                               <  SRR4302540.1902382/94‑1 (MQ=60)
                GGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGACGGCGATGGTCTGTATCGTACTGCGTTGCTGTTCATGGGCCGCGACGCACTGCCC                                                                              >  SRR4302540.7736119/1‑94 (MQ=60)
                GGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCC                                                                              >  SRR4302540.4366919/1‑94 (MQ=60)
                GGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCC                                                                              >  SRR4302540.147149/1‑94 (MQ=60)
                GGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCC                                                                              >  SRR4302540.9392604/1‑94 (MQ=60)
                 GTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCTCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCA                                                                             <  SRR4302540.8687265/94‑1 (MQ=60)
                 GTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCGGCGACGCACTGCCCC                                                                             >  SRR4302540.5959862/1‑94 (MQ=60)
                  TTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCAC                                                                            <  SRR4302540.8859386/94‑1 (MQ=60)
                  TTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCAC                                                                            >  SRR4302540.6325561/1‑94 (MQ=60)
                   TCGTGACGTTGAAGGTGCAGAGCGTAACGGCTCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACT                                                                           <  SRR4302540.10102303/94‑1 (MQ=60)
                   TCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACT                                                                           <  SRR4302540.1049355/94‑1 (MQ=60)
                   TCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACT                                                                           <  SRR4302540.1049058/94‑1 (MQ=60)
                     GTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCCGTTCATGGGCCGCGACGCACTGCCCACTGA                                                                         >  SRR4302540.8310607/1‑94 (MQ=60)
                     CTGACGTTGAAGGTGCAGAGCGTAACGGCTCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGA                                                                         <  SRR4302540.5388809/94‑1 (MQ=60)
                       GACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGCAG                                                                       >  SRR4302540.1034812/1‑94 (MQ=60)
                       GACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAG                                                                       <  SRR4302540.4744339/94‑1 (MQ=60)
                         CGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAA                                                                     >  SRR4302540.5741565/1‑94 (MQ=60)
                         CGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAA                                                                     >  SRR4302540.4133053/1‑94 (MQ=60)
                         CGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGACGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAA                                                                     <  SRR4302540.8981252/94‑1 (MQ=60)
                          GTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAG                                                                    >  SRR4302540.2850532/1‑94 (MQ=60)
                          GTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAG                                                                    <  SRR4302540.5436565/94‑1 (MQ=60)
                          GTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAG                                                                    >  SRR4302540.9302266/1‑94 (MQ=60)
                          GTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAG                                                                    >  SRR4302540.4226084/1‑94 (MQ=60)
                           TTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGA                                                                   >  SRR4302540.5614994/1‑94 (MQ=60)
                            TGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAA                                                                  <  SRR4302540.4217080/94‑1 (MQ=60)
                             GAAGGTGCAGAGCGTAACGGCTCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTGCATGGGCCGCGACGCACTGCCCACTGAAGAAGAAC                                                                 <  SRR4302540.3565751/94‑1 (MQ=60)
                             GAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAAC                                                                 <  SRR4302540.3565750/94‑1 (MQ=60)
                             GAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAAC                                                                 <  SRR4302540.7654497/94‑1 (MQ=60)
                               AGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAG                                                               <  SRR4302540.6294509/94‑1 (MQ=60)
                               AGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAG                                                               <  SRR4302540.469311/94‑1 (MQ=60)
                               AGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAG                                                               >  SRR4302540.7910199/1‑94 (MQ=60)
                               AGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAG                                                               <  SRR4302540.7077563/94‑1 (MQ=60)
                               AGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAG                                                               <  SRR4302540.6484991/94‑1 (MQ=60)
                                GGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGT                                                              <  SRR4302540.175487/94‑1 (MQ=60)
                                GGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGT                                                              >  SRR4302540.8915986/1‑94 (MQ=60)
                                GGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGT                                                              >  SRR4302540.8247032/1‑94 (MQ=60)
                                  TGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTT                                                            <  SRR4302540.10087759/94‑1 (MQ=60)
                                    GAGAGCGTAACTGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGC                                                          <  SRR4302540.4799047/94‑1 (MQ=60)
                                    CAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGC                                                          <  SRR4302540.4799048/94‑1 (MQ=60)
                                     AGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCT                                                         >  SRR4302540.7752201/1‑94 (MQ=60)
                                     AGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCT                                                         >  SRR4302540.5576944/1‑94 (MQ=60)
                                     AGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCT                                                         <  SRR4302540.2725637/94‑1 (MQ=60)
                                     AGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCT                                                         <  SRR4302540.3517813/94‑1 (MQ=60)
                                      GAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTG                                                        <  SRR4302540.6186293/94‑1 (MQ=60)
                                         CGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTT                                                     >  SRR4302540.6192655/1‑94 (MQ=60)
                                          GTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTA                                                    >  SRR4302540.9590351/1‑94 (MQ=60)
                                          GTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTA                                                    <  SRR4302540.7263217/94‑1 (MQ=60)
                                            AACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACA                                                  >  SRR4302540.5067746/1‑94 (MQ=60)
                                             ACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAA                                                 >  SRR4302540.7944977/1‑94 (MQ=60)
                                             ACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAA                                                 <  SRR4302540.6039563/94‑1 (MQ=60)
                                              CGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAA                                                <  SRR4302540.4241477/94‑1 (MQ=60)
                                              CGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAA                                                <  SRR4302540.4241478/94‑1 (MQ=60)
                                               GGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAG                                               <  SRR4302540.7873716/94‑1 (MQ=60)
                                                GCGCTGAAGGCGTTTGTCTGTATCGGACTGAGTTCCTGTTCATGGACCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGC                                              <  SRR4302540.3572607/94‑1 (MQ=60)
                                                GCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGC                                              <  SRR4302540.9165031/94‑1 (MQ=60)
                                                 CGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCA                                             >  SRR4302540.2951696/1‑94 (MQ=60)
                                                 CGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCA                                             >  SRR4302540.915370/1‑94 (MQ=60)
                                                 CGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCA                                             >  SRR4302540.6183260/1‑94 (MQ=60)
                                                  GCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAG                                            >  SRR4302540.8404692/1‑94 (MQ=60)
                                                  GCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAG                                            <  SRR4302540.7238675/94‑1 (MQ=60)
                                                  GCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAG                                            <  SRR4302540.2606415/94‑1 (MQ=60)
                                                    TGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTG                                          <  SRR4302540.8661015/94‑1 (MQ=60)
                                                    TGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTG                                          <  SRR4302540.8502244/94‑1 (MQ=60)
                                                    TGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTG                                          <  SRR4302540.5121065/94‑1 (MQ=60)
                                                     GAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGG                                         <  SRR4302540.5933818/94‑1 (MQ=60)
                                                     GAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGG                                         >  SRR4302540.1064004/1‑94 (MQ=60)
                                                      AAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGC                                        >  SRR4302540.7634587/1‑94 (MQ=60)
                                                      AAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGC                                        >  SRR4302540.1649111/1‑94 (MQ=60)
                                                      AAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGC                                        <  SRR4302540.1303695/94‑1 (MQ=60)
                                                      AAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGC                                        >  SRR4302540.9636435/1‑94 (MQ=60)
                                                      AAGGCGGTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGC                                        <  SRR4302540.7008675/94‑1 (MQ=60)
                                                       AGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCT                                       >  SRR4302540.4502446/1‑94 (MQ=60)
                                                       AGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCT                                       >  SRR4302540.8707500/1‑94 (MQ=60)
                                                       AGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCT                                       <  SRR4302540.6492881/94‑1 (MQ=60)
                                                        GGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTG                                      <  SRR4302540.653043/94‑1 (MQ=60)
                                                        GGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTG                                      <  SRR4302540.120828/94‑1 (MQ=60)
                                                        GGCGTTGGGCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTA                                      >  SRR4302540.7424241/1‑94 (MQ=60)
                                                          CGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAA                                    >  SRR4302540.5998202/1‑94 (MQ=60)
                                                          CGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAA                                    <  SRR4302540.3036604/94‑1 (MQ=60)
                                                           GTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAG                                   >  SRR4302540.4118717/1‑94 (MQ=60)
                                                           GTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAG                                   >  SRR4302540.6572159/1‑94 (MQ=60)
                                                           GTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAG                                   <  SRR4302540.910142/94‑1 (MQ=60)
                                                           GTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAG                                   >  SRR4302540.4118718/1‑94 (MQ=60)
                                                           GTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAG                                   >  SRR4302540.7560285/1‑94 (MQ=60)
                                                           GTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAG                                   >  SRR4302540.5799428/1‑94 (MQ=60)
                                                           GTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAG                                   >  SRR4302540.5799429/1‑94 (MQ=60)
                                                             TGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCG                                 >  SRR4302540.3712708/1‑94 (MQ=60)
                                                             TGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCG                                 <  SRR4302540.9490424/94‑1 (MQ=60)
                                                               GTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTG                               >  SRR4302540.960191/1‑94 (MQ=60)
                                                                TCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGT                              <  SRR4302540.2451950/94‑1 (MQ=60)
                                                                 CTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTG                             <  SRR4302540.9285417/94‑1 (MQ=60)
                                                                 CTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTG                             <  SRR4302540.9878178/94‑1 (MQ=60)
                                                                 CTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTG                             <  SRR4302540.6461761/94‑1 (MQ=60)
                                                                  TGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGG                            <  SRR4302540.9608558/94‑1 (MQ=60)
                                                                  TGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGG                            <  SRR4302540.5470778/94‑1 (MQ=60)
                                                                   GTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGC                           >  SRR4302540.2483593/1‑94 (MQ=60)
                                                                    TATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCT                          >  SRR4302540.1754543/1‑94 (MQ=60)
                                                                     ATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTG                         >  SRR4302540.1070827/1‑94 (MQ=60)
                                                                     ATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTC                         >  SRR4302540.9143720/1‑94 (MQ=60)
                                                                      TCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCG                        <  SRR4302540.9718618/94‑1 (MQ=60)
                                                                       CGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGACGCGTGTGGCTCGC                       >  SRR4302540.6169870/1‑94 (MQ=60)
                                                                       CGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGC                       >  SRR4302540.5775628/1‑94 (MQ=60)
                                                                        GTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACACAGCAGTGGCTGAAGCGTGTGGCTCTCA                      >  SRR4302540.4806399/1‑94 (MQ=60)
                                                                        GTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCA                      >  SRR4302540.1683023/1‑94 (MQ=60)
                                                                        GTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCA                      >  SRR4302540.4806400/1‑94 (MQ=60)
                                                                         TACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCAACTGAAGAAGAACAGATTGCTGCTTGCAAAGAAGTGGCTGAAGAGTGTGGCTCGCTA                     >  SRR4302540.1626542/1‑94 (MQ=60)
                                                                          ACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAG                    <  SRR4302540.3894267/94‑1 (MQ=60)
                                                                          ACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAG                    <  SRR4302540.3293443/94‑1 (MQ=60)
                                                                          ACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAG                    <  SRR4302540.4599499/94‑1 (MQ=60)
                                                                          ACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAG                    >  SRR4302540.1811843/1‑94 (MQ=60)
                                                                          ACTGAGTTCCAGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAG                    <  SRR4302540.3293442/94‑1 (MQ=60)
                                                                           CTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGC                   <  SRR4302540.10026289/94‑1 (MQ=60)
                                                                           CTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGC                   >  SRR4302540.6706324/1‑94 (MQ=60)
                                                                            TGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCG                  <  SRR4302540.4129323/94‑1 (MQ=60)
                                                                            TGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCG                  <  SRR4302540.6196468/94‑1 (MQ=60)
                                                                            TGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCG                  <  SRR4302540.8769379/94‑1 (MQ=60)
                                                                            TGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCG                  <  SRR4302540.1749431/94‑1 (MQ=60)
                                                                             GAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGG                 >  SRR4302540.6033203/1‑94 (MQ=60)
                                                                             GAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGG                 >  SRR4302540.6033202/1‑94 (MQ=60)
                                                                              AGTTCCTGTTCATGGGCCGCGACGCACTGCTGACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGT                <  SRR4302540.9609913/94‑1 (MQ=60)
                                                                              AGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGT                <  SRR4302540.9609912/94‑1 (MQ=60)
                                                                              AGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGT                <  SRR4302540.5286227/94‑1 (MQ=60)
                                                                              AGTGCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGT                <  SRR4302540.3241984/94‑1 (MQ=60)
                                                                               GTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTT               >  SRR4302540.4907679/1‑94 (MQ=60)
                                                                                TTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTA              >  SRR4302540.4232909/1‑94 (MQ=60)
                                                                                 TCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAT             <  SRR4302540.2553566/94‑1 (MQ=60)
                                                                                 TCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAT             <  SRR4302540.2245394/94‑1 (MQ=60)
                                                                                 TCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAT             <  SRR4302540.3325581/94‑1 (MQ=60)
                                                                                  CCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATC            <  SRR4302540.6103036/94‑1 (MQ=60)
                                                                                   CTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCG           >  SRR4302540.808674/1‑94 (MQ=60)
                                                                                    TGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGT          <  SRR4302540.7278774/94‑1 (MQ=60)
                                                                                    TGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGT          <  SRR4302540.9543689/94‑1 (MQ=60)
                                                                                    CGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGT          <  SRR4302540.8296983/94‑1 (MQ=60)
                                                                                    CGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGT          <  SRR4302540.1007026/94‑1 (MQ=60)
                                                                                     GTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTT         <  SRR4302540.1325406/94‑1 (MQ=60)
                                                                                     GTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTT         >  SRR4302540.6621699/1‑94 (MQ=60)
                                                                                     GTTCATGGGCCGCGACGAACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTT         <  SRR4302540.9067953/94‑1 (MQ=60)
                                                                                      TTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGGTATCGTTC        >  SRR4302540.4489438/1‑94 (MQ=60)
                                                                                        CATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGT      >  SRR4302540.57736/1‑94 (MQ=60)
                                                                                         ATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAgggttcgtc     >  SRR4302540.2173326/1‑85 (MQ=60)
                                                                                         ATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGTA     >  SRR4302540.288389/1‑94 (MQ=60)
                                                                                          TGGGCAGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGTAC    <  SRR4302540.4850121/94‑1 (MQ=60)
                                                                                           GGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGTACC   >  SRR4302540.3497092/1‑94 (MQ=60)
                                                                                           GGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGTACC   <  SRR4302540.8831934/94‑1 (MQ=60)
                                                                                           GGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGTACC   <  SRR4302540.8820369/94‑1 (MQ=60)
                                                                                            GGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCTGTTATCGTTCGTACCA  >  SRR4302540.9030462/1‑94 (MQ=60)
                                                                                             |                                                                                            
GCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGACCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGTACCA  >  NC_000913/2532900‑2533085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑