Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,532,993 A→G D303G (GAC→GGC)  ptsI → PEP‑protein phosphotransferase system enzyme I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,532,9930AG100.0% 730.8 / NA 226D303G (GAC→GGC) ptsIPEP‑protein phosphotransferase system enzyme I
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (104/122);  total (104/122)

Only 100 of 232 total aligned reads displayed.

CTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGACCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGTA  >  NC_000913/2532903‑2533082
                                                                                          |                                                                                         
cTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGcc                                                                                         >  2:3246974/1‑93 (MQ=255)
cTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGcc                                                                                         <  2:3723692/93‑1 (MQ=255)
cTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGcc                                                                                         <  2:5208463/93‑1 (MQ=255)
cTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGcc                                                                                         <  1:223252/93‑1 (MQ=255)
 tAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCcg                                                                                        >  1:3724222/1‑93 (MQ=255)
  aaCATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCcgc                                                                                       >  2:4843495/1‑93 (MQ=255)
   aCATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCcgcg                                                                                      >  1:3159500/1‑93 (MQ=255)
     aTTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGAc                                                                                    >  1:3407227/1‑93 (MQ=255)
     aTTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGAc                                                                                    <  1:4176176/93‑1 (MQ=255)
      ttGGTACGTTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGGTACTGTTCATGGGCCgcggcg                                                                                   >  2:3922079/1‑93 (MQ=255)
      ttGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGAcc                                                                                   >  2:4111034/1‑92 (MQ=255)
      ttGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACg                                                                                   >  1:971319/1‑93 (MQ=255)
         gTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCAc                                                                                <  1:3981007/93‑1 (MQ=255)
         gTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCAc                                                                                <  2:3107112/93‑1 (MQ=255)
         gTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCAc                                                                                <  2:1441547/93‑1 (MQ=255)
           aCGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTg                                                                              >  2:1723476/1‑93 (MQ=255)
            cGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGc                                                                             <  2:562425/93‑1 (MQ=255)
            cGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGc                                                                             <  2:5927891/93‑1 (MQ=255)
            cGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGc                                                                             <  2:748079/93‑1 (MQ=255)
              gTTGGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGCAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGccc                                                                           <  2:1945562/93‑1 (MQ=255)
              gTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGccc                                                                           <  2:1360655/93‑1 (MQ=255)
                  gTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTg                                                                       >  1:2666814/1‑93 (MQ=255)
                   tGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTga                                                                      >  2:5134898/1‑93 (MQ=255)
                    gACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaa                                                                     <  2:2416468/93‑1 (MQ=255)
                    gACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaa                                                                     >  1:658344/1‑93 (MQ=255)
                      cGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaaga                                                                   <  1:4652362/93‑1 (MQ=255)
                       gTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaagaa                                                                  >  1:1341206/1‑93 (MQ=255)
                       gTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaagaa                                                                  <  1:5693878/93‑1 (MQ=255)
                        ttGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaagaag                                                                 >  1:5187781/1‑93 (MQ=255)
                        ttGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaagaag                                                                 >  1:5084756/1‑93 (MQ=255)
                          gAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaagaagaa                                                               >  1:1086869/1‑93 (MQ=255)
                          gAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaagaagaa                                                               <  1:6247269/93‑1 (MQ=255)
                          gAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTgaagaagaa                                                               >  1:2578720/1‑93 (MQ=255)
                           aaGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAAc                                                              <  2:4904838/93‑1 (MQ=255)
                           aaGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAAc                                                              <  2:5397770/93‑1 (MQ=255)
                            aGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACa                                                             <  2:3486134/93‑1 (MQ=255)
                               tGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGtt                                                          <  1:2019569/93‑1 (MQ=255)
                                gCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGttt                                                         <  1:6148452/93‑1 (MQ=255)
                                  agagCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTtgc                                                       <  2:3013205/93‑1 (MQ=255)
                                  agagCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTtgc                                                       <  1:4292343/93‑1 (MQ=255)
                                   gagCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTtgct                                                      >  1:5367355/1‑93 (MQ=255)
                                   gagCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTtgct                                                      <  1:5793405/93‑1 (MQ=255)
                                   gagCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTtgct                                                      >  2:1939364/1‑93 (MQ=255)
                                     gCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTtgctgc                                                    >  2:4627616/1‑93 (MQ=255)
                                      cGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTgctgct                                                   <  2:4364495/93‑1 (MQ=255)
                                        tAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTa                                                 >  1:4254551/1‑93 (MQ=255)
                                          aCGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACa                                               >  1:589520/1‑93 (MQ=255)
                                          aCGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAAAAGAACAGTTTGCTGCTTACa                                               <  2:3664184/93‑1 (MQ=255)
                                                cTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAg                                         >  2:2215214/1‑93 (MQ=255)
                                                cTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAg                                         <  1:4607465/93‑1 (MQ=255)
                                                 tGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGt                                        <  1:3675727/93‑1 (MQ=255)
                                                 tGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGt                                        <  2:2852709/93‑1 (MQ=255)
                                                 tGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGt                                        <  2:2590212/93‑1 (MQ=255)
                                                  gAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTg                                       <  2:1528873/93‑1 (MQ=255)
                                                  gAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTg                                       <  2:1609850/93‑1 (MQ=255)
                                                  gAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTg                                       <  2:3304466/93‑1 (MQ=255)
                                                    aGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGc                                     >  2:73890/1‑93 (MQ=255)
                                                    aGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGc                                     <  1:333429/93‑1 (MQ=255)
                                                     ggCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCt                                    <  1:1468576/93‑1 (MQ=255)
                                                     ggCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCt                                    >  1:1792345/1‑93 (MQ=255)
                                                      gCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTg                                   >  1:264115/1‑93 (MQ=255)
                                                       cGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGa                                  >  1:4893094/1‑93 (MQ=255)
                                                        gTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGaa                                 <  1:1886224/93‑1 (MQ=255)
                                                         ttGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAg                                >  2:4692951/1‑93 (MQ=255)
                                                          tGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGc                               >  1:6071152/1‑93 (MQ=255)
                                                          tGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGc                               >  2:6065138/1‑93 (MQ=255)
                                                           ggTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCg                              >  2:2676102/1‑93 (MQ=255)
                                                           ggTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCg                              >  1:3484973/1‑93 (MQ=255)
                                                            gTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCgt                             <  1:1260806/93‑1 (MQ=255)
                                                            gTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCgt                             <  1:74904/93‑1 (MQ=255)
                                                              cTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCgtgt                           <  1:4530498/93‑1 (MQ=255)
                                                               tGTATCGTACTGAGTTCCTGTTCATGGGCCGCGAGGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGtgtg                          >  1:1001188/1‑93 (MQ=255)
                                                               tGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGtgtg                          <  2:702907/93‑1 (MQ=255)
                                                               tGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGtgtg                          <  1:2373039/93‑1 (MQ=255)
                                                               tGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGtgtg                          <  2:4872294/93‑1 (MQ=255)
                                                               tGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGtgtg                          <  2:1254556/93‑1 (MQ=255)
                                                                  aTCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCt                       <  2:2521062/93‑1 (MQ=255)
                                                                   tCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTc                      >  2:1073172/1‑93 (MQ=255)
                                                                     gTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGc                    >  1:4561310/1‑93 (MQ=255)
                                                                      tACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCa                   >  2:4979589/1‑93 (MQ=255)
                                                                       aCTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCaa                  >  2:4075760/1‑93 (MQ=255)
                                                                       aCTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCaa                  >  1:2405045/1‑93 (MQ=255)
                                                                       aCTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCaa                  <  1:1134367/93‑1 (MQ=255)
                                                                        cTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCaac                 >  2:65707/1‑92 (MQ=255)
                                                                          gAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAgag               >  2:6250620/1‑91 (MQ=255)
                                                                            gTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGt             >  2:5729598/1‑93 (MQ=255)
                                                                               ccTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAt          <  2:3487597/93‑1 (MQ=255)
                                                                               ccTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGGTAt          >  2:3349199/1‑93 (MQ=255)
                                                                                cTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGGTAtc         >  2:3966720/1‑93 (MQ=255)
                                                                                 tGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcg        >  2:4290594/1‑93 (MQ=255)
                                                                                 tGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcg        >  1:4396731/1‑93 (MQ=255)
                                                                                  gTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcgt       >  1:5037801/1‑93 (MQ=255)
                                                                                   ttCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcgtt      <  1:3044418/93‑1 (MQ=255)
                                                                                   ttCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcgtt      >  2:2100433/1‑93 (MQ=255)
                                                                                    tCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcgttc     <  1:4419666/93‑1 (MQ=255)
                                                                                    tCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcgttc     <  1:3900261/93‑1 (MQ=255)
                                                                                     cATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcgttcg    >  1:4980682/1‑93 (MQ=255)
                                                                                     cATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTAtcgttcg    >  2:2196810/1‑93 (MQ=255)
                                                                                       tGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGTa  <  1:1609177/93‑1 (MQ=255)
                                                                                       tGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGTa  <  2:1758486/93‑1 (MQ=255)
                                                                                          |                                                                                         
CTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGACCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGTA  >  NC_000913/2532903‑2533082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGACCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGTACCA  >  NC_000913/2532902‑2533085
                                                                                           |                                                                                            
GCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCC                                                                                            <  SRR4302533.5135782/94‑1 (MQ=60)
GCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGGAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCC                                                                                            <  SRR4302533.522954/94‑1 (MQ=60)
 CTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCG                                                                                           >  SRR4302533.3747071/1‑94 (MQ=60)
 CTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCG                                                                                           <  SRR4302533.224644/94‑1 (MQ=60)
  TAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGC                                                                                          >  SRR4302533.4199107/1‑94 (MQ=60)
  TAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGC                                                                                          >  SRR4302533.5773438/1‑94 (MQ=60)
   AACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCG                                                                                         >  SRR4302533.3178944/1‑94 (MQ=60)
     CATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGAC                                                                                       >  SRR4302533.3428271/1‑94 (MQ=60)
     CATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGAC                                                                                       >  SRR4302533.4267264/1‑94 (MQ=60)
      ATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACG                                                                                      <  SRR4302533.4201479/94‑1 (MQ=60)
      ATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACG                                                                                      >  SRR4302533.977021/1‑94 (MQ=60)
          GTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACT                                                                                  <  SRR4302533.4005244/94‑1 (MQ=60)
           TACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTG                                                                                 <  SRR4302533.2027793/94‑1 (MQ=60)
               GTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCA                                                                             >  SRR4302533.1454502/1‑94 (MQ=60)
                  CGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTG                                                                          >  SRR4302533.2683177/1‑94 (MQ=60)
                  CGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTG                                                                          >  SRR4302533.5796608/1‑94 (MQ=60)
                  CGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTG                                                                          <  SRR4302533.2422499/94‑1 (MQ=60)
                    TGACGTTGAAGGTGCAGAGCGTAACGGCTCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAA                                                                        <  SRR4302533.3462466/94‑1 (MQ=60)
                    TGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAA                                                                        >  SRR4302533.662225/1‑94 (MQ=60)
                       CGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAA                                                                     <  SRR4302533.6144866/94‑1 (MQ=60)
                       CGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAA                                                                     <  SRR4302533.4680554/94‑1 (MQ=60)
                       CGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAA                                                                     >  SRR4302533.2394826/1‑94 (MQ=60)
                       CGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAA                                                                     <  SRR4302533.3786226/94‑1 (MQ=60)
                       CGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAA                                                                     >  SRR4302533.1349281/1‑94 (MQ=60)
                        GTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAG                                                                    >  SRR4302533.5219091/1‑94 (MQ=60)
                        GTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAG                                                                    <  SRR4302533.5728261/94‑1 (MQ=60)
                        GTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAG                                                                    >  SRR4302533.5115484/1‑94 (MQ=60)
                        GTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGGCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAG                                                                    >  SRR4302533.409974/1‑94 (MQ=60)
                         TTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGA                                                                   <  SRR4302533.3730457/94‑1 (MQ=60)
                         TTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGA                                                                   >  SRR4302533.2836890/1‑94 (MQ=60)
                         TTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGA                                                                   <  SRR4302533.4456325/94‑1 (MQ=60)
                          TGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAA                                                                  >  SRR4302533.1093274/1‑94 (MQ=60)
                          TGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAA                                                                  >  SRR4302533.2594589/1‑94 (MQ=60)
                          TGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCACGGGCCGCGACGCACTGCCCACTGAAGAAGAA                                                                  <  SRR4302533.1162250/94‑1 (MQ=60)
                           GAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAAC                                                                 <  SRR4302533.4431706/94‑1 (MQ=60)
                           GAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAAC                                                                 <  SRR4302533.4834821/94‑1 (MQ=60)
                           GAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAAC                                                                 <  SRR4302533.6284655/94‑1 (MQ=60)
                                TGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTT                                                            <  SRR4302533.2032007/94‑1 (MQ=60)
                                 GCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTG                                                           <  SRR4302533.6185466/94‑1 (MQ=60)
                                  CAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGC                                                          <  SRR4302533.5084353/94‑1 (MQ=60)
                                   AGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCTCACTGAAGAAGAACAGTTTGCT                                                         <  SRR4302533.5188701/94‑1 (MQ=60)
                                   AGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCT                                                         <  SRR4302533.4580798/94‑1 (MQ=60)
                                   AGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCT                                                         <  SRR4302533.4974291/94‑1 (MQ=60)
                                   AGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCT                                                         <  SRR4302533.5188702/94‑1 (MQ=60)
                                   AGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCT                                                         <  SRR4302533.4318158/94‑1 (MQ=60)
                                   AGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCT                                                         >  SRR4302533.5399600/1‑94 (MQ=60)
                                    GAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTG                                                        >  SRR4302533.2752867/1‑94 (MQ=60)
                                    GAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTG                                                        >  SRR4302533.2213000/1‑94 (MQ=60)
                                    GAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTG                                                        <  SRR4302533.5828405/94‑1 (MQ=60)
                                       CGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTT                                                     <  SRR4302533.3358131/94‑1 (MQ=60)
                                        GTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTA                                                    <  SRR4302533.3358824/94‑1 (MQ=60)
                                        GTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTA                                                    >  SRR4302533.4280206/1‑94 (MQ=60)
                                         TAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTAC                                                   <  SRR4302533.1345627/94‑1 (MQ=60)
                                          AACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACA                                                  >  SRR4302533.3349799/1‑94 (MQ=60)
                                          AACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACA                                                  >  SRR4302533.593003/1‑94 (MQ=60)
                                          AACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACA                                                  <  SRR4302533.1781049/94‑1 (MQ=60)
                                             GGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAG                                               <  SRR4302533.1510088/94‑1 (MQ=60)
                                              GCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGC                                              <  SRR4302533.4440190/94‑1 (MQ=60)
                                               CGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCA                                             >  SRR4302533.1087818/1‑94 (MQ=60)
                                                GCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAG                                            <  SRR4302533.5924506/94‑1 (MQ=60)
                                                 CTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGT                                           <  SRR4302533.6190725/94‑1 (MQ=60)
                                                 CTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGT                                           <  SRR4302533.4635370/94‑1 (MQ=60)
                                                 CTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGT                                           >  SRR4302533.3103970/1‑94 (MQ=60)
                                                 CTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGT                                           <  SRR4302533.3595452/94‑1 (MQ=60)
                                                 CTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGT                                           >  SRR4302533.4365074/1‑94 (MQ=60)
                                                  TGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTG                                          >  SRR4302533.1698381/1‑94 (MQ=60)
                                                  TGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTG                                          <  SRR4302533.3698315/94‑1 (MQ=60)
                                                     AGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCT                                       >  SRR4302533.1803248/1‑94 (MQ=60)
                                                     AGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCT                                       >  SRR4302533.2428380/1‑94 (MQ=60)
                                                     AGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCT                                       <  SRR4302533.335468/94‑1 (MQ=60)
                                                      GGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTG                                      <  SRR4302533.1477431/94‑1 (MQ=60)
                                                      GGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTG                                      >  SRR4302533.265717/1‑94 (MQ=60)
                                                       GCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGA                                     >  SRR4302533.4922754/1‑94 (MQ=60)
                                                        CGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAA                                    <  SRR4302533.2637862/94‑1 (MQ=60)
                                                        CGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAA                                    <  SRR4302533.4669138/94‑1 (MQ=60)
                                                        CGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAA                                    <  SRR4302533.979017/94‑1 (MQ=60)
                                                         GTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACGAAGCAGTGGCTGAAG                                   <  SRR4302533.5001615/94‑1 (MQ=60)
                                                         GTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAG                                   <  SRR4302533.1897744/94‑1 (MQ=60)
                                                         GTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAG                                   <  SRR4302533.6157924/94‑1 (MQ=60)
                                                          TTGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGC                                  >  SRR4302533.6107883/1‑94 (MQ=60)
                                                           TGGTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCG                                 >  SRR4302533.3506465/1‑94 (MQ=60)
                                                             GTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTG                               <  SRR4302533.1268346/94‑1 (MQ=60)
                                                             GTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTG                               <  SRR4302533.75391/94‑1 (MQ=60)
                                                             GTCTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTG                               >  SRR4302533.2366802/1‑94 (MQ=60)
                                                               CTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGAGGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTG                             >  SRR4302533.1007033/1‑94 (MQ=60)
                                                               CTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTG                             <  SRR4302533.6339372/94‑1 (MQ=60)
                                                               CTGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTG                             <  SRR4302533.4557932/94‑1 (MQ=60)
                                                                TGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGG                            >  SRR4302533.5058279/1‑94 (MQ=60)
                                                                TGTATCGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGG                            <  SRR4302533.2387724/94‑1 (MQ=60)
                                                                     CGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGC                       >  SRR4302533.4588973/1‑94 (MQ=60)
                                                                     CGTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGC                       >  SRR4302533.830779/1‑94 (MQ=60)
                                                                      GTACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCA                      >  SRR4302533.4610102/1‑94 (MQ=60)
                                                                       TACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAA                     >  SRR4302533.2419898/1‑94 (MQ=60)
                                                                       TACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAA                     >  SRR4302533.3701661/1‑94 (MQ=60)
                                                                        ACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAG                    <  SRR4302533.1141111/94‑1 (MQ=60)
                                                                        ACTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAG                    <  SRR4302533.749715/94‑1 (MQ=60)
                                                                         CTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGC                   >  SRR4302533.745654/1‑94 (MQ=60)
                                                                         CTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGC                   >  SRR4302533.4313881/1‑94 (MQ=60)
                                                                         CTGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGC                   <  SRR4302533.1114177/94‑1 (MQ=60)
                                                                          TGAGTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCG                  >  SRR4302533.5705779/1‑94 (MQ=60)
                                                                             GTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTT               <  SRR4302533.2111407/94‑1 (MQ=60)
                                                                             GTTCCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTT               <  SRR4302533.2659737/94‑1 (MQ=60)
                                                                                CCTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATC            >  SRR4302533.5951529/1‑94 (MQ=60)
                                                                                 CTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCG           >  SRR4302533.4423277/1‑94 (MQ=60)
                                                                                 CTGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCG           <  SRR4302533.5381401/94‑1 (MQ=60)
                                                                                  TGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGT          >  SRR4302533.5068307/1‑94 (MQ=60)
                                                                                  TGTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGT          >  SRR4302533.460953/1‑94 (MQ=60)
                                                                                   GTTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTT         >  SRR4302533.3996062/1‑94 (MQ=60)
                                                                                    TTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTC        <  SRR4302533.1291841/94‑1 (MQ=60)
                                                                                    TTCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTC        <  SRR4302533.3063053/94‑1 (MQ=60)
                                                                                     TCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCG       <  SRR4302533.3924066/94‑1 (MQ=60)
                                                                                     TCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCG       <  SRR4302533.4446387/94‑1 (MQ=60)
                                                                                     TCATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCG       >  SRR4302533.5010880/1‑94 (MQ=60)
                                                                                      CATGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGT      >  SRR4302533.186708/1‑94 (MQ=60)
                                                                                        TGGGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGTAC    <  SRR4302533.1618937/94‑1 (MQ=60)
                                                                                          GGCCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGTACCA  <  SRR4302533.2929793/94‑1 (MQ=60)
                                                                                           |                                                                                            
GCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGACCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTACAAAGCAGTGGCTGAAGCGTGTGGCTCGCAAGCGGTTATCGTTCGTACCA  >  NC_000913/2532902‑2533085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑