Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,582,538 A→G G336G (GGT→GGC)  yffG ← putative oxidoreductase Fe‑S subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,582,5380AG100.0% 627.7 / NA 200G336G (GGT→GGC) yffGputative oxidoreductase Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (77/123);  total (77/123)

Only 100 of 217 total aligned reads displayed.

GAGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAAT  >  NC_000913/2582448‑2582627
                                                                                          |                                                                                         
gAGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGcc                                                                                         >  1:7351036/1‑93 (MQ=255)
gAGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGcc                                                                                         >  2:5430471/1‑93 (MQ=255)
  gCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTg                                                                                       <  1:3084504/93‑1 (MQ=255)
  gCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTg                                                                                       >  2:4808829/1‑93 (MQ=255)
   cAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGc                                                                                      <  1:5644339/93‑1 (MQ=255)
   cAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGc                                                                                      >  1:2497402/1‑93 (MQ=255)
    aaGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGcc                                                                                     >  1:8301605/1‑92 (MQ=255)
     aGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCAc                                                                                    >  2:5233560/1‑93 (MQ=255)
      gCCACCGATTTCTGGATGGCGATCGTACACCGTCCCCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCAcc                                                                                   <  1:2207547/93‑1 (MQ=255)
      gCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCAcc                                                                                   <  1:8224784/93‑1 (MQ=255)
        cACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGa                                                                                 <  2:5398144/93‑1 (MQ=255)
         aCCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGAt                                                                                >  2:2145099/1‑93 (MQ=255)
         aCCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGAt                                                                                >  1:8907059/1‑93 (MQ=255)
          ccGATTTCTGGATGGCGATCGTACACCGTCCCCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATa                                                                               <  2:233583/93‑1 (MQ=255)
           cGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACACCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATaa                                                                              <  1:8450903/93‑1 (MQ=255)
           cGATTGCTGGATGTCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATaa                                                                              <  2:7619144/93‑1 (MQ=255)
            gATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGACAGCCCTGCCGGGCCTGCACCGATAAt                                                                             >  2:1811504/1‑93 (MQ=255)
             aTTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATc                                                                            <  1:6061065/93‑1 (MQ=255)
             aTTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATc                                                                            <  2:1450347/93‑1 (MQ=255)
               ttCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGc                                                                          >  2:2846470/1‑93 (MQ=255)
                  tGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCAc                                                                       <  1:6795164/93‑1 (MQ=255)
                  tGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCAc                                                                       <  1:5772646/93‑1 (MQ=255)
                   ggATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCAcc                                                                      >  2:961244/1‑93 (MQ=255)
                   ggATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGACTGCACCGATAATCGCCAcc                                                                      >  2:6869006/1‑93 (MQ=255)
                         cGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTg                                                                <  2:2242285/93‑1 (MQ=255)
                           aTCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTc                                                              >  2:390346/1‑93 (MQ=255)
                               tACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCCCt                                                          >  2:3682268/1‑93 (MQ=255)
                               tACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACt                                                          >  2:4682491/1‑93 (MQ=255)
                               tACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACt                                                          >  1:1504138/1‑93 (MQ=255)
                                acacCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACtt                                                         <  1:4588878/93‑1 (MQ=255)
                                  cccGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTg                                                       <  1:1224430/92‑1 (MQ=255)
                                  acCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTg                                                       >  1:7507499/1‑93 (MQ=255)
                                   ccGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTgg                                                      <  2:4582667/93‑1 (MQ=255)
                                    cGTCTCCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGt                                                     <  1:4967416/93‑1 (MQ=255)
                                     gTCCCCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTg                                                    <  1:2703161/93‑1 (MQ=255)
                                     gTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTg                                                    <  1:4501645/93‑1 (MQ=255)
                                        ccccccACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACa                                                 <  2:4471681/92‑1 (MQ=255)
                                        ccccccACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACa                                                 <  2:5434633/92‑1 (MQ=255)
                                         cccccACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACAt                                                <  2:197984/93‑1 (MQ=255)
                                         cccccACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACAt                                                >  2:4810641/1‑93 (MQ=255)
                                         cccccACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACAt                                                <  2:380736/93‑1 (MQ=255)
                                         cccccACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCAACTTTGGTGACAt                                                >  2:339334/1‑93 (MQ=255)
                                           cccACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATgg                                              <  1:565952/93‑1 (MQ=255)
                                           cccACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATgg                                              >  2:8071502/1‑93 (MQ=255)
                                           cccACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGAAATgg                                              >  1:2439538/1‑93 (MQ=255)
                                           cccACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCAACCCGCTTGTCCACTTTGGTGGCATgg                                              >  1:3772677/1‑93 (MQ=255)
                                            ccACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGc                                             >  1:7260050/1‑93 (MQ=255)
                                              aCGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCtt                                           >  2:4337017/1‑93 (MQ=255)
                                              aCGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCtt                                           >  2:5702364/1‑93 (MQ=255)
                                               cGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCGCTTTGGTGACATGGCTTa                                          <  1:785966/93‑1 (MQ=255)
                                               cGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTa                                          <  1:2651015/93‑1 (MQ=255)
                                                  cATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGt                                       <  1:4448347/93‑1 (MQ=255)
                                                  cATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGt                                       <  1:1382565/93‑1 (MQ=255)
                                                   aTTGCGGGTCAGAACATCGGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTc                                      <  2:985272/93‑1 (MQ=255)
                                                   aTTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTc                                      >  1:7451170/1‑93 (MQ=255)
                                                   aTTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTc                                      <  2:6449613/93‑1 (MQ=255)
                                                    ttGCGGTTCAGACCACCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCa                                     <  1:6582211/93‑1 (MQ=255)
                                                    ttGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCa                                     <  1:5514003/93‑1 (MQ=255)
                                                     tGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAg                                    <  2:592558/93‑1 (MQ=255)
                                                     tGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAg                                    >  1:6863634/1‑93 (MQ=255)
                                                      gCGGGTCATAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAgg                                   <  1:8026453/93‑1 (MQ=255)
                                                       cGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCCCTTTGGTGACATGGCTTAAGTCAGGa                                  <  1:6478886/93‑1 (MQ=255)
                                                       cGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGa                                  <  2:6946609/93‑1 (MQ=255)
                                                       cGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGa                                  <  2:10037484/93‑1 (MQ=255)
                                                       cGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGa                                  >  2:3089330/1‑93 (MQ=255)
                                                         ggTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACg                                <  1:4296744/93‑1 (MQ=255)
                                                          gTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGc                               >  1:771714/1‑93 (MQ=255)
                                                             aGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCaa                            <  1:5464759/93‑1 (MQ=255)
                                                             aGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCaa                            >  2:7177632/1‑93 (MQ=255)
                                                               aaCATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAAcc                          <  1:529608/93‑1 (MQ=255)
                                                                aCATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCCACCt                         >  2:2936895/1‑93 (MQ=255)
                                                                 cATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCtt                        <  2:7368215/93‑1 (MQ=255)
                                                                  ctCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCttt                       <  2:8364036/92‑1 (MQ=255)
                                                                  aTCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCttt                       <  1:9487801/93‑1 (MQ=255)
                                                                   tCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTc                      >  1:9630429/1‑93 (MQ=255)
                                                                   tCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTc                      <  2:966239/93‑1 (MQ=255)
                                                                   tCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTc                      >  2:9927836/1‑93 (MQ=255)
                                                                   tCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTc                      <  1:9966303/93‑1 (MQ=255)
                                                                    ccGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCg                     <  1:6522880/93‑1 (MQ=255)
                                                                      gCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGcc                   >  1:3004538/1‑93 (MQ=255)
                                                                        acaGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCaa                 <  2:6139283/93‑1 (MQ=255)
                                                                        acaGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCaa                 <  1:3504233/93‑1 (MQ=255)
                                                                          aGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAAcc               >  2:9035408/1‑92 (MQ=255)
                                                                           ggCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGc              <  2:2046485/93‑1 (MQ=255)
                                                                            gCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGcc             <  1:1671801/93‑1 (MQ=255)
                                                                            gCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGcc             <  2:7730539/93‑1 (MQ=255)
                                                                            gCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGcc             <  1:3745549/93‑1 (MQ=255)
                                                                             ccAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCt            <  1:1344289/93‑1 (MQ=255)
                                                                              caacccTGCCGCGCCTGCGCCGATAAGCGCCTCCGGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGCCGCCACCCTTTCGCCAACGCCTg           <  1:3067184/90‑1 (MQ=255)
                                                                              cAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTg           <  2:4521699/93‑1 (MQ=255)
                                                                                gCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGAt         <  1:9536547/93‑1 (MQ=255)
                                                                                gCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGAt         <  1:1688064/93‑1 (MQ=255)
                                                                                gCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACAGGGCTTAAGTCAGGACGCAAACCTTTCGCCAACGCCTGAt         <  1:1743024/93‑1 (MQ=255)
                                                                                 cccTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATc        >  1:9045791/1‑93 (MQ=255)
                                                                                   cTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTg      >  1:5020500/1‑93 (MQ=255)
                                                                                    tGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGa     >  2:7941505/1‑93 (MQ=255)
                                                                                      ccGGGCCTTCCCCGATAAACCCCCCCCCCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGaaa   <  2:1689549/93‑1 (MQ=255)
                                                                                      ccGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGaaa   <  2:8301604/93‑1 (MQ=255)
                                                                                      ccGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGaaa   <  2:2420637/93‑1 (MQ=255)
                                                                                       cGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAAt  <  2:4758615/93‑1 (MQ=255)
                                                                                          |                                                                                         
GAGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAAT  >  NC_000913/2582448‑2582627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGTGAGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAG  >  NC_000913/2582445‑2582631
                                                                                             |                                                                                             
AGTGACCGAGCCACCGATTTCTGGATGGCGATCCTACCCCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGG                                                                                               <  SRR4302540.5685160/94‑1 (MQ=60)
  TGAGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCC                                                                                             >  SRR4302540.7433036/1‑94 (MQ=60)
   GAGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCT                                                                                            <  SRR4302540.322576/94‑1 (MQ=60)
   GAGCAAGCCACCGATTTCTGGATGGCGATCGGTCACCGGCCCCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCT                                                                                            <  SRR4302540.9577313/94‑1 (MQ=60)
     GCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGC                                                                                          >  SRR4302540.2524327/1‑94 (MQ=60)
     GCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGC                                                                                          <  SRR4302540.3118349/94‑1 (MQ=60)
      CAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCC                                                                                         >  SRR4302540.8397992/1‑94 (MQ=60)
      CAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCA                                                                                         <  SRR4302540.5706459/94‑1 (MQ=60)
       AAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCAC                                                                                        >  SRR4302540.1611283/1‑94 (MQ=60)
       AAGCAACCGATTTCTGGATGGCGATCGTAAACCGTCAGCCCCACGCCATTGCGGGTCAGAACATCAGCACAGGCCAGCCCTGCCGGGGCTGCAC                                                                                        >  SRR4302540.1611284/1‑94 (MQ=60)
        AGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACC                                                                                       >  SRR4302540.8977310/1‑94 (MQ=60)
         GCCACCGATTTCTGGATGGCGATCGTACACCGTCCCCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCG                                                                                      <  SRR4302540.2231390/94‑1 (MQ=60)
         GCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCG                                                                                      <  SRR4302540.8320396/94‑1 (MQ=60)
          CCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGA                                                                                     >  SRR4302540.1924108/1‑94 (MQ=60)
           CACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGAT                                                                                    >  SRR4302540.9010196/1‑94 (MQ=60)
            ACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATA                                                                                   >  SRR4302540.138915/1‑94 (MQ=60)
            ACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATA                                                                                   >  SRR4302540.6051849/1‑94 (MQ=60)
             CCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAA                                                                                  >  SRR4302540.5684615/1‑94 (MQ=60)
              CGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAAT                                                                                 <  SRR4302540.5094628/94‑1 (MQ=60)
              CGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAAT                                                                                 <  SRR4302540.3737250/94‑1 (MQ=60)
              CGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACACCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAAT                                                                                 <  SRR4302540.8549014/94‑1 (MQ=60)
                ATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCG                                                                               <  SRR4302540.6128427/94‑1 (MQ=60)
                ATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCG                                                                               >  SRR4302540.6879684/1‑94 (MQ=60)
                  TTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCC                                                                             <  SRR4302540.5524640/94‑1 (MQ=60)
                    CTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCCCCGATAATCGCCAC                                                                           >  SRR4302540.3000958/1‑94 (MQ=60)
                    CTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCAC                                                                           >  SRR4302540.3000956/1‑94 (MQ=60)
                    CTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCAC                                                                           <  SRR4302540.1746854/94‑1 (MQ=60)
                     TGGATGTCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACC                                                                          <  SRR4302540.5050255/94‑1 (MQ=60)
                     TGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACC                                                                          <  SRR4302540.5836412/94‑1 (MQ=60)
                     TGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACC                                                                          <  SRR4302540.6870316/94‑1 (MQ=60)
                      GGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCC                                                                         >  SRR4302540.3798639/1‑94 (MQ=60)
                             GATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTC                                                                  <  SRR4302540.232978/94‑1 (MQ=60)
                              ATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCC                                                                 <  SRR4302540.5146376/94‑1 (MQ=60)
                                CGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCAC                                                               >  SRR4302540.8103657/1‑94 (MQ=60)
                                CGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCAC                                                               >  SRR4302540.4674739/1‑94 (MQ=60)
                                 GTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACT                                                              >  SRR4302540.1520729/1‑94 (MQ=60)
                                   ACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTT                                                            <  SRR4302540.4638285/94‑1 (MQ=60)
                                    CACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTG                                                           >  SRR4302540.7591413/1‑94 (MQ=60)
                                    CACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTG                                                           >  SRR4302540.1434497/1‑94 (MQ=60)
                                     CCCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGG                                                          <  SRR4302540.1236954/94‑1 (MQ=60)
                                       CGTCTCCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTG                                                        <  SRR4302540.5021148/94‑1 (MQ=60)
                                       CGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTG                                                        <  SRR4302540.840159/94‑1 (MQ=60)
                                        ctccCCCCCGCGCCATTGCGGGTCAGAACAACCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGA                                                       <  SRR4302540.7487464/90‑1 (MQ=60)
                                        GTCCCCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGA                                                       <  SRR4302540.7909458/94‑1 (MQ=60)
                                        GTCCCCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGA                                                       <  SRR4302540.2732325/94‑1 (MQ=60)
                                        GTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGA                                                       <  SRR4302540.4550443/94‑1 (MQ=60)
                                           CCCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACAT                                                    <  SRR4302540.7391118/94‑1 (MQ=60)
                                             CCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGAAATGG                                                  >  SRR4302540.2465805/1‑94 (MQ=60)
                                             CCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCAACCCGCTTGTCCACTTTGGTGGCATGG                                                  >  SRR4302540.3813836/1‑94 (MQ=60)
                                              CCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGC                                                 <  SRR4302540.572108/94‑1 (MQ=60)
                                              CCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGC                                                 <  SRR4302540.572109/94‑1 (MQ=60)
                                              CCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGC                                                 >  SRR4302540.7341062/1‑94 (MQ=60)
                                                CACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTT                                               <  SRR4302540.2731407/94‑1 (MQ=60)
                                                CACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCACCACCCGCTTGTCCACTTTGGTGACATGGCTT                                               >  SRR4302540.2600760/1‑94 (MQ=60)
                                                  CGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCGCTTTGGTGACATGGCTTAA                                             <  SRR4302540.794323/94‑1 (MQ=60)
                                                  CGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAA                                             <  SRR4302540.2679776/94‑1 (MQ=60)
                                                  CGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAA                                             <  SRR4302540.7991767/94‑1 (MQ=60)
                                                   GCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAG                                            <  SRR4302540.3952344/94‑1 (MQ=60)
                                                     CATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTC                                          <  SRR4302540.4496557/94‑1 (MQ=60)
                                                     CATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTC                                          >  SRR4302540.7534222/1‑94 (MQ=60)
                                                     CATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTC                                          <  SRR4302540.1397322/94‑1 (MQ=60)
                                                     CATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCCATAATCGCCACACGCTTGTCCACTTTGGGGACATGGCTTAAGTC                                          >  SRR4302540.660582/1‑94 (MQ=60)
                                                       TTGCGGTTCAGACCACCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAG                                        <  SRR4302540.6655121/94‑1 (MQ=60)
                                                       TTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAG                                        >  SRR4302540.6939700/1‑94 (MQ=60)
                                                       TTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAG                                        <  SRR4302540.5574733/94‑1 (MQ=60)
                                                       TTGCGGGACAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAG                                        <  SRR4302540.5574732/94‑1 (MQ=60)
                                                         GCGGGTCATAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGA                                      <  SRR4302540.8119795/94‑1 (MQ=60)
                                                          CGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCCCTTTGGTGACATGGCTTAAGTCAGGAC                                     <  SRR4302540.6550962/94‑1 (MQ=60)
                                                          CGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGAC                                     <  SRR4302540.6551227/94‑1 (MQ=60)
                                                           GGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACG                                    >  SRR4302540.5719793/1‑94 (MQ=60)
                                                            GGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGC                                   >  SRR4302540.779924/1‑94 (MQ=60)
                                                            GGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGC                                   <  SRR4302540.10114033/94‑1 (MQ=60)
                                                            GGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGC                                   <  SRR4302540.4343501/94‑1 (MQ=60)
                                                              GCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCA                                 <  SRR4302540.7536070/94‑1 (MQ=60)
                                                               CAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAC                                >  SRR4302540.8363547/1‑94 (MQ=60)
                                                                AGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAAC                               <  SRR4302540.5524910/94‑1 (MQ=60)
                                                                 GAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACC                              <  SRR4302540.9795644/94‑1 (MQ=60)
                                                                  AACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCT                             <  SRR4302540.535329/94‑1 (MQ=60)
                                                                    CATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTT                           <  SRR4302540.6052179/94‑1 (MQ=60)
                                                                    CATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTT                           >  SRR4302540.9616131/1‑94 (MQ=60)
                                                                     ATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTC                          >  SRR4302540.9743910/1‑94 (MQ=60)
                                                                     ATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTC                          <  SRR4302540.9600285/94‑1 (MQ=60)
                                                                     ATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTC                          >  SRR4302540.1699857/1‑94 (MQ=60)
                                                                      TCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCG                         <  SRR4302540.4619600/94‑1 (MQ=60)
                                                                      TCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCG                         <  SRR4302540.10082578/94‑1 (MQ=60)
                                                                      CCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCG                         <  SRR4302540.4543886/94‑1 (MQ=60)
                                                                       CCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGC                        <  SRR4302540.6595325/94‑1 (MQ=60)
                                                                        CGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCC                       <  SRR4302540.7090524/94‑1 (MQ=60)
                                                                        CGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCC                       >  SRR4302540.3037621/1‑94 (MQ=60)
                                                                        CGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCC                       <  SRR4302540.6747494/94‑1 (MQ=60)
                                                                           ccagcCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAAC                    <  SRR4302540.8189839/89‑1 (MQ=60)
                                                                           CCAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAAC                    <  SRR4302540.9031078/94‑1 (MQ=60)
                                                                           ACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAAC                    <  SRR4302540.3542404/94‑1 (MQ=60)
                                                                            CAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACG                   <  SRR4302540.3997301/94‑1 (MQ=60)
                                                                             AGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGC                  >  SRR4302540.1316458/1‑94 (MQ=60)
                                                                               GCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCT                <  SRR4302540.3786423/94‑1 (MQ=60)
                                                                               GCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCT                <  SRR4302540.9339974/94‑1 (MQ=60)
                                                                               GCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCT                <  SRR4302540.1689980/94‑1 (MQ=60)
                                                                               GCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCT                <  SRR4302540.7742842/94‑1 (MQ=60)
                                                                                CCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTG               <  SRR4302540.1358396/94‑1 (MQ=60)
                                                                                 CAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGA              <  SRR4302540.2467018/94‑1 (MQ=60)
                                                                                 CAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGA              <  SRR4302540.309810/94‑1 (MQ=60)
                                                                                 CAACCCTGCCGCGCCTGCGCCGATAAGCGCCTCCGGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGCCGCCACCCTTTCGCCAACGCCTGA              <  SRR4302540.3100834/94‑1 (MQ=57)
                                                                                   GCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATC            <  SRR4302540.1706373/94‑1 (MQ=60)
                                                                                   GCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATC            <  SRR4302540.1706372/94‑1 (MQ=60)
                                                                                   GCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATC            >  SRR4302540.9154374/1‑94 (MQ=60)
                                                                                   GCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATC            <  SRR4302540.9649426/94‑1 (MQ=60)
                                                                                   GCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACAGGGCTTAAGTCAGGACGCAAACCTTTCGCCAACGCCTGATC            <  SRR4302540.1761887/94‑1 (MQ=60)
                                                                                    CCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGTCATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCT           <  SRR4302540.3065139/94‑1 (MQ=60)
                                                                                    CCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCT           <  SRR4302540.9698578/94‑1 (MQ=60)
                                                                                     CCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTG          >  SRR4302540.5074848/1‑94 (MQ=60)
                                                                                     CCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTAAGGACGCCAACCTTTCGCCAACGCCTGATCTG          <  SRR4302540.6321797/94‑1 (MQ=60)
                                                                                          CGGTCCTGCACCGTTAATCGCCGCCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATG     <  SRR4302540.70831/94‑1 (MQ=60)
                                                                                          CGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATG     <  SRR4302540.4488115/94‑1 (MQ=60)
                                                                                             GCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAG  <  SRR4302540.9395136/94‑1 (MQ=60)
                                                                                             GCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAG  <  SRR4302540.7440722/94‑1 (MQ=60)
                                                                                             |                                                                                             
AGTGAGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAG  >  NC_000913/2582445‑2582631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑