Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,582,538 A→G G336G (GGT→GGC)  yffG ← putative oxidoreductase Fe‑S subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,582,5380AG100.0% 638.2 / NA 201G336G (GGT→GGC) yffGputative oxidoreductase Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (91/110);  total (91/110)

Only 100 of 208 total aligned reads displayed.

GAGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAAT  >  NC_000913/2582448‑2582627
                                                                                          |                                                                                         
gAGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGcc                                                                                         >  1:258887/1‑93 (MQ=255)
 aGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCt                                                                                        >  1:986476/1‑93 (MQ=255)
  gCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTg                                                                                       >  1:5859954/1‑93 (MQ=255)
    aaGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCa                                                                                     >  1:4556513/1‑93 (MQ=255)
     aGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCAc                                                                                    >  2:2830875/1‑93 (MQ=255)
      gCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCAcc                                                                                   >  2:79805/1‑93 (MQ=255)
        cACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGa                                                                                 >  2:4971468/1‑93 (MQ=255)
        cACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGa                                                                                 >  2:1815620/1‑93 (MQ=255)
        cACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGa                                                                                 <  2:3658984/93‑1 (MQ=255)
          ccGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATa                                                                               >  1:848981/1‑93 (MQ=255)
          ccGATTTCTGGATGGCGATCGTACACCGTCACCCCCAAGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATa                                                                               >  2:5244407/1‑93 (MQ=255)
           cGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATaa                                                                              <  2:2538931/93‑1 (MQ=255)
           cGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATaa                                                                              <  1:5271434/93‑1 (MQ=255)
            gATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAAt                                                                             <  1:6225997/93‑1 (MQ=255)
             aTTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATc                                                                            <  2:226972/93‑1 (MQ=255)
              tttCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCg                                                                           >  1:1137780/1‑93 (MQ=255)
              tttCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCg                                                                           >  2:4073247/1‑93 (MQ=255)
                tCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGcc                                                                         <  2:2191908/93‑1 (MQ=255)
                 cTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCa                                                                        <  2:3489806/93‑1 (MQ=255)
                  tGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCAc                                                                       >  2:2556919/1‑93 (MQ=255)
                  tGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCAc                                                                       <  1:4291292/93‑1 (MQ=255)
                     aTGGCGATCGTACACCGTCTCCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCg                                                                    >  2:6165992/1‑93 (MQ=255)
                      tGGCGAGCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGc                                                                   <  2:1332054/93‑1 (MQ=255)
                       ggCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCt                                                                  <  1:2258535/93‑1 (MQ=255)
                        gCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCtt                                                                 <  1:5067922/93‑1 (MQ=255)
                        gCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCtt                                                                 <  1:2461161/93‑1 (MQ=255)
                         cGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTg                                                                >  1:1025629/1‑93 (MQ=255)
                          gATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGt                                                               >  2:3774987/1‑93 (MQ=255)
                          gATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGt                                                               >  2:4252058/1‑93 (MQ=255)
                           aTCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTc                                                              <  2:6046142/93‑1 (MQ=255)
                           aTCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTc                                                              >  1:5060367/1‑93 (MQ=255)
                           aTCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTc                                                              >  1:1596007/1‑93 (MQ=255)
                           aTCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTc                                                              >  2:3498260/1‑93 (MQ=255)
                            tCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTcc                                                             <  2:5924155/93‑1 (MQ=255)
                            tCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTcc                                                             >  1:1089486/1‑93 (MQ=255)
                             cGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCa                                                            <  1:6236154/93‑1 (MQ=255)
                             cGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCa                                                            <  2:4859152/93‑1 (MQ=255)
                             cGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCa                                                            <  1:2079895/93‑1 (MQ=255)
                              gTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCAc                                                           >  2:3885101/1‑93 (MQ=255)
                                acacCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACtt                                                         >  2:3923011/1‑93 (MQ=255)
                                 cacCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACttt                                                        <  1:1781965/93‑1 (MQ=255)
                                 cacCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACttt                                                        <  1:5556440/93‑1 (MQ=255)
                                  acCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTg                                                       >  1:3359645/1‑93 (MQ=255)
                                   ccGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTgg                                                      >  2:1549607/1‑93 (MQ=255)
                                   ccGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTgg                                                      >  2:3176487/1‑93 (MQ=255)
                                    cGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGt                                                     <  1:2421521/93‑1 (MQ=255)
                                      tCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGa                                                   >  2:5846467/1‑93 (MQ=255)
                                      tCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGa                                                   >  1:5650482/1‑93 (MQ=255)
                                         cccccACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACAt                                                >  1:6047366/1‑93 (MQ=255)
                                          ccccACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATg                                               <  1:5805173/93‑1 (MQ=255)
                                           cccACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATgg                                              >  1:2536759/1‑93 (MQ=255)
                                            ccACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGc                                             <  1:2330654/93‑1 (MQ=255)
                                              aCGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCtt                                           >  1:5290741/1‑93 (MQ=255)
                                               cGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTa                                          <  1:4328102/93‑1 (MQ=255)
                                               cGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTa                                          >  2:5117415/1‑93 (MQ=255)
                                                gCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTaa                                         >  2:4499374/1‑93 (MQ=255)
                                                gCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTaa                                         >  1:4750212/1‑93 (MQ=255)
                                                 ccATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAg                                        <  2:3815148/93‑1 (MQ=255)
                                                   aTTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTc                                      >  2:5614833/1‑93 (MQ=255)
                                                   aTTCCGCGTCAGAACACCCGCACAGCCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTc                                      <  2:5705570/93‑1 (MQ=25)
                                                    ttGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCa                                     <  1:2720145/93‑1 (MQ=255)
                                                    ttGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGGGACATGGCTTAAGTCa                                     >  1:566248/1‑93 (MQ=255)
                                                     tGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAg                                    <  1:175502/93‑1 (MQ=255)
                                                      gCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCTACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAgg                                   <  1:4912339/93‑1 (MQ=255)
                                                       cGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGa                                  <  2:1826166/93‑1 (MQ=255)
                                                       cGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGa                                  <  1:3492923/93‑1 (MQ=255)
                                                         ggTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACg                                <  1:381566/93‑1 (MQ=255)
                                                           tCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGcc                              >  2:334928/1‑93 (MQ=255)
                                                              gAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAAc                           >  2:1943231/1‑93 (MQ=255)
                                                               aaCATCCGCGCAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAAcc                          <  1:3770725/93‑1 (MQ=255)
                                                                 cATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCtt                        >  1:2098582/1‑93 (MQ=255)
                                                                  aTCCGCACAGACCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCttt                       <  2:3138224/93‑1 (MQ=255)
                                                                   tCCGCACAGGCCGGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTc                      <  1:5431619/93‑1 (MQ=255)
                                                                   tCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTCAGTCCGGACGCCAACCCTTc                      >  1:2849547/1‑93 (MQ=37)
                                                                   tCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTc                      <  2:3260827/93‑1 (MQ=255)
                                                                        ccaGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCaa                 <  2:642607/92‑1 (MQ=255)
                                                                         caGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAAc                <  2:1665341/93‑1 (MQ=255)
                                                                         caGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAAc                <  2:1597127/93‑1 (MQ=255)
                                                                          aGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACg               >  1:3006671/1‑93 (MQ=255)
                                                                          aGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTCGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACg               >  1:1402026/1‑93 (MQ=255)
                                                                           ggCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGc              <  2:5755965/93‑1 (MQ=255)
                                                                           ggCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGc              >  2:86558/1‑93 (MQ=255)
                                                                            gCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGcc             <  1:2024575/93‑1 (MQ=255)
                                                                            gCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGcc             <  1:3960967/93‑1 (MQ=255)
                                                                               aGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGa          <  1:644529/93‑1 (MQ=255)
                                                                               aGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGa          >  2:3823114/1‑93 (MQ=255)
                                                                               aGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGa          <  2:3052249/93‑1 (MQ=255)
                                                                                gCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGAt         <  1:589124/93‑1 (MQ=255)
                                                                                gCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGAt         >  1:5737264/1‑93 (MQ=255)
                                                                                 cccTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATc        <  1:1297217/93‑1 (MQ=255)
                                                                                 cccTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATc        >  2:923208/1‑93 (MQ=255)
                                                                                  ccTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCt       >  1:2919644/1‑93 (MQ=255)
                                                                                  ccTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCt       <  1:1972549/93‑1 (MQ=255)
                                                                                   cTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTg      >  2:4721609/1‑93 (MQ=255)
                                                                                    tGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGa     <  1:5469391/93‑1 (MQ=255)
                                                                                    tGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGa     >  2:903398/1‑93 (MQ=255)
                                                                                      ccGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGaaa   <  2:3118102/93‑1 (MQ=255)
                                                                                      ccGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGaaa   <  1:3247592/93‑1 (MQ=255)
                                                                                       cGGGCCTGCACCGATAATCGCCACCCGCTTGTCCGCTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAAt  <  1:5188572/93‑1 (MQ=255)
                                                                                       cGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAAt  >  2:2045728/1‑93 (MQ=255)
                                                                                          |                                                                                         
GAGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAAT  >  NC_000913/2582448‑2582627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGAGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAG  >  NC_000913/2582447‑2582631
                                                                                           |                                                                                             
TGAGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCC                                                                                             >  SRR4302533.260460/1‑94 (MQ=60)
 GAGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCT                                                                                            >  SRR4302533.992251/1‑94 (MQ=60)
  AGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTG                                                                                           >  SRR4302533.5895380/1‑94 (MQ=60)
    CAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCA                                                                                         >  SRR4302533.4584146/1‑94 (MQ=60)
     AAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCAC                                                                                        >  SRR4302533.5182085/1‑94 (MQ=60)
      AGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACC                                                                                       >  SRR4302533.1606278/1‑94 (MQ=60)
         CACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGAT                                                                                    >  SRR4302533.5809419/1‑94 (MQ=60)
          ACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATA                                                                                   >  SRR4302533.853950/1‑94 (MQ=60)
           CCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAA                                                                                  <  SRR4302533.5984328/94‑1 (MQ=60)
            CGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAAT                                                                                 <  SRR4302533.2142996/94‑1 (MQ=60)
            CGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAAT                                                                                 <  SRR4302533.5303049/94‑1 (MQ=60)
             GATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATC                                                                                <  SRR4302533.6263299/94‑1 (MQ=60)
              ATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCG                                                                               >  SRR4302533.1144548/1‑94 (MQ=60)
              ATTTCTGGATGGCGATCGTACACCGACACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCG                                                                               >  SRR4302533.5322570/1‑94 (MQ=60)
                  CTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCAC                                                                           >  SRR4302533.2576004/1‑94 (MQ=60)
                   TGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACC                                                                          <  SRR4302533.4317098/94‑1 (MQ=60)
                   TGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACC                                                                          >  SRR4302533.5750430/1‑94 (MQ=60)
                   TGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCAATAATCGCCACC                                                                          <  SRR4302533.4717160/94‑1 (MQ=60)
                     GATGGCGAGCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCG                                                                        <  SRR4302533.524447/94‑1 (MQ=60)
                     GATGGCCATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCG                                                                        <  SRR4302533.3665384/94‑1 (MQ=60)
                       TGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCT                                                                      <  SRR4302533.2306141/94‑1 (MQ=60)
                        GGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTT                                                                     <  SRR4302533.3506094/94‑1 (MQ=60)
                        GGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTT                                                                     <  SRR4302533.2272510/94‑1 (MQ=60)
                        GGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTT                                                                     <  SRR4302533.2477725/94‑1 (MQ=60)
                         GCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTG                                                                    <  SRR4302533.5098527/94‑1 (MQ=60)
                         GCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTG                                                                    <  SRR4302533.2476347/94‑1 (MQ=60)
                         GCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTG                                                                    >  SRR4302533.1031656/1‑94 (MQ=60)
                           GATCGTACACCGTCCCCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTC                                                                  <  SRR4302533.4820705/94‑1 (MQ=60)
                           GATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTC                                                                  >  SRR4302533.1778385/1‑94 (MQ=60)
                           GATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTC                                                                  >  SRR4302533.1605666/1‑94 (MQ=60)
                           GATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTC                                                                  >  SRR4302533.5090944/1‑94 (MQ=60)
                            ATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCC                                                                 <  SRR4302533.2292809/94‑1 (MQ=60)
                            ATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCC                                                                 >  SRR4302533.1095917/1‑94 (MQ=60)
                            ATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCC                                                                 >  SRR4302533.5054654/1‑94 (MQ=60)
                              CGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCAC                                                               <  SRR4302533.6273497/94‑1 (MQ=60)
                              CGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCAC                                                               <  SRR4302533.2092692/94‑1 (MQ=60)
                                 ACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTT                                                            <  SRR4302533.2473804/94‑1 (MQ=60)
                                 ACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTT                                                            <  SRR4302533.5497033/94‑1 (MQ=60)
                                 ACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTT                                                            <  SRR4302533.708141/94‑1 (MQ=60)
                                  CACCGTCGCCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTG                                                           <  SRR4302533.4021418/94‑1 (MQ=60)
                                  CACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTG                                                           <  SRR4302533.5590019/94‑1 (MQ=60)
                                  CACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTG                                                           <  SRR4302533.1792806/94‑1 (MQ=60)
                                  CACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTG                                                           >  SRR4302533.3380402/1‑94 (MQ=60)
                                     CGTCCCCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTG                                                        <  SRR4302533.592052/94‑1 (MQ=60)
                                     CGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTG                                                        <  SRR4302533.2436471/94‑1 (MQ=60)
                                      GTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGA                                                       >  SRR4302533.5684638/1‑94 (MQ=60)
                                        CACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACA                                                     >  SRR4302533.3098089/1‑94 (MQ=60)
                                         ACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACAT                                                    >  SRR4302533.6084009/1‑94 (MQ=60)
                                          CCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATG                                                   >  SRR4302533.6278922/1‑94 (MQ=60)
                                           CCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGG                                                  >  SRR4302533.1860994/1‑94 (MQ=60)
                                           CCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGG                                                  <  SRR4302533.5689479/94‑1 (MQ=60)
                                           CCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGG                                                  >  SRR4302533.2552376/1‑94 (MQ=60)
                                           CCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGG                                                  <  SRR4302533.5840228/94‑1 (MQ=60)
                                            CCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCCGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGC                                                 <  SRR4302533.626994/94‑1 (MQ=60)
                                             CCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCT                                                <  SRR4302533.2345084/94‑1 (MQ=60)
                                             CCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCT                                                <  SRR4302533.1998115/94‑1 (MQ=60)
                                              CACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTT                                               >  SRR4302533.5322422/1‑94 (MQ=60)
                                                CGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAA                                             >  SRR4302533.4779013/1‑94 (MQ=60)
                                                CGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAA                                             <  SRR4302533.4354175/94‑1 (MQ=60)
                                                   CATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTC                                          >  SRR4302533.1057326/1‑94 (MQ=60)
                                                    ATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCA                                         <  SRR4302533.1367331/94‑1 (MQ=60)
                                                    ATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGGGACATGGCTTAAGTCA                                         >  SRR4302533.569602/1‑94 (MQ=60)
                                                     TTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCGGGGCCTGCACCGATAATCGCCTCCCGCTTGTCGTCTTTGGTGACATGGCTTAAGTCAG                                        <  SRR4302533.985398/94‑1 (MQ=60)
                                                     TTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAG                                        <  SRR4302533.235636/94‑1 (MQ=60)
                                                     TTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAG                                        <  SRR4302533.2736841/94‑1 (MQ=60)
                                                      TGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGG                                       <  SRR4302533.2899410/94‑1 (MQ=60)
                                                      TGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGG                                       <  SRR4302533.3364856/94‑1 (MQ=60)
                                                      TGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGG                                       <  SRR4302533.176607/94‑1 (MQ=60)
                                                       GCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCTACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGA                                      <  SRR4302533.4942097/94‑1 (MQ=60)
                                                       GCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGA                                      >  SRR4302533.5528047/1‑94 (MQ=60)
                                                        CGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGAC                                     <  SRR4302533.3514463/94‑1 (MQ=60)
                                                        CGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGAC                                     <  SRR4302533.2087726/94‑1 (MQ=60)
                                                         GGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTGCACTTTGGTGACATGGCTTAAGTCAGGACG                                    >  SRR4302533.6095994/1‑94 (MQ=60)
                                                         GGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACG                                    <  SRR4302533.6329227/94‑1 (MQ=60)
                                                          GGTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGC                                   <  SRR4302533.383868/94‑1 (MQ=60)
                                                           GTCAGAACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCC                                  <  SRR4302533.5342307/94‑1 (MQ=60)
                                                                AACATCCGCGCAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCT                             <  SRR4302533.3793843/94‑1 (MQ=60)
                                                                 ACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTT                            <  SRR4302533.5091676/94‑1 (MQ=60)
                                                                 ACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTT                            >  SRR4302533.2111529/1‑94 (MQ=60)
                                                                 ACATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTT                            <  SRR4302533.2993581/94‑1 (MQ=60)
                                                                  CATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTT                           >  SRR4302533.5863745/1‑94 (MQ=60)
                                                                   ATCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTCAGTCCGGACGCCAACCCTTC                          >  SRR4302533.2867080/1‑94 (MQ=60)
                                                                    TCCGCACAGGCCGGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCG                         <  SRR4302533.5464328/94‑1 (MQ=60)
                                                                    TCCGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCG                         <  SRR4302533.2094068/94‑1 (MQ=60)
                                                                      CGCACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCC                       >  SRR4302533.2845524/1‑94 (MQ=60)
                                                                        CACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAA                     >  SRR4302533.1143424/1‑94 (MQ=60)
                                                                        CACAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAA                     <  SRR4302533.3939091/94‑1 (MQ=60)
                                                                         ACAGGCCAGCCCTGTCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAAC                    >  SRR4302533.1282411/1‑94 (MQ=60)
                                                                          CAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACG                   >  SRR4302533.3025062/1‑94 (MQ=60)
                                                                          CAGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTCGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACG                   >  SRR4302533.1410481/1‑94 (MQ=60)
                                                                           AGGCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGC                  >  SRR4302533.530473/1‑94 (MQ=60)
                                                                             GCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCT                <  SRR4302533.2037075/94‑1 (MQ=60)
                                                                             GCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCT                >  SRR4302533.4344110/1‑94 (MQ=60)
                                                                             GCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCT                <  SRR4302533.3985100/94‑1 (MQ=60)
                                                                             GCCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTACACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCT                >  SRR4302533.4469177/1‑94 (MQ=60)
                                                                              CCAGCCCTGCCGGGCCTGCACCGATAATTGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTA               >  SRR4302533.931425/1‑94 (MQ=60)
                                                                              CCAGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTG               <  SRR4302533.563278/94‑1 (MQ=60)
                                                                                AGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCTAACCTTTCGCCAACGCCTGAT             <  SRR4302533.3807000/94‑1 (MQ=60)
                                                                                AGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGAT             >  SRR4302533.5771892/1‑94 (MQ=60)
                                                                                AGCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGAT             <  SRR4302533.648324/94‑1 (MQ=60)
                                                                                 GCCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATC            <  SRR4302533.592606/94‑1 (MQ=60)
                                                                                  CCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCT           >  SRR4302533.2937532/1‑94 (MQ=60)
                                                                                  CCCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCT           <  SRR4302533.1305026/94‑1 (MQ=60)
                                                                                   CCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTG          <  SRR4302533.1984621/94‑1 (MQ=60)
                                                                                   CCTGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTG          <  SRR4302533.3645035/94‑1 (MQ=60)
                                                                                     TGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAA        <  SRR4302533.265109/94‑1 (MQ=60)
                                                                                     TGCCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAA        <  SRR4302533.5502354/94‑1 (MQ=60)
                                                                                       CCGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAAT      <  SRR4302533.3267621/94‑1 (MQ=60)
                                                                                        CGGGCCTGCACCGATAATCGCCACCCGCTTGTCCGCTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATG     <  SRR4302533.5219884/94‑1 (MQ=60)
                                                                                        CGGGCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCATAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATG     >  SRR4302533.3631828/1‑94 (MQ=60)
                                                                                           GCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAG  <  SRR4302533.3660027/94‑1 (MQ=60)
                                                                                           GCCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAG  <  SRR4302533.408724/94‑1 (MQ=60)
                                                                                           |                                                                                             
TGAGCAAGCCACCGATTTCTGGATGGCGATCGTACACCGTCACCCCCACGCCATTGCGGGTCAGAACATCCGCACAGGCCAGCCCTGCCGGACCTGCACCGATAATCGCCACCCGCTTGTCCACTTTGGTGACATGGCTTAAGTCAGGACGCCAACCTTTCGCCAACGCCTGATCTGAAATGTAG  >  NC_000913/2582447‑2582631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑