Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,586,558	A→G	Q315R (CAG→CGG)	acrD →	AcrD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,586,558	0	A	G	99.1%	683.2 / ‑3.3	213	Q315R (CAG→CGG)	acrD	AcrD
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (99/112); total (100/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

Only 100 of 221 total aligned reads displayed.

CCAGCCTCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCAGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAAAACGCTGC  >  NC_000913/2586467‑2586647

  cgccTCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTa                                                                                        <  2:379577/92‑1 (MQ=255)

   gccTCCTGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTAt                                                                                       <  1:4562558/93‑1 (MQ=255)

   gccTCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTAt                                                                                       >  2:9884605/1‑93 (MQ=255)

   gccTCCGGGCTGGGGGTAAAACTGGCCGCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTAt                                                                                       <  1:6037954/93‑1 (MQ=255)

    ccTCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTAtt                                                                                      >  1:3098043/1‑93 (MQ=255)

     cTCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTTAATCGTCTCGACGAGCTGGCGCGGTAttt                                                                                     >  1:5496501/1‑93 (MQ=255)

      tCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTc                                                                                    >  1:1063559/1‑93 (MQ=255)

      tCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTc                                                                                    >  1:6443541/1‑93 (MQ=255)

      tCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTc                                                                                    <  2:8382761/93‑1 (MQ=255)

         gggCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCg                                                                                 >  2:8135971/1‑93 (MQ=255)

          ggCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGc                                                                                >  1:9541598/1‑93 (MQ=255)

          ggCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGc                                                                                <  1:4358046/93‑1 (MQ=255)

           gCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCa                                                                               >  2:7973996/1‑93 (MQ=255)

           gCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCa                                                                               <  1:4505258/93‑1 (MQ=255)

           gCTGGGGGTAAAACTGGCCTCCGGCACTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCa                                                                               <  2:878726/93‑1 (MQ=255)

             tGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATg                                                                             >  2:6233968/1‑93 (MQ=255)

               gggaTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCAGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGa                                                                           <  1:8926022/93‑1 (MQ=255)

                gggTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGAc                                                                          <  1:9389098/93‑1 (MQ=255)

                      aaCTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAAt                                                                    >  1:3617886/1‑93 (MQ=255)

                      aaCTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAAt                                                                    >  1:9557728/1‑93 (MQ=255)

                       aCTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATa                                                                   <  1:8016012/93‑1 (MQ=255)

                        cTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATAc                                                                  <  2:779902/93‑1 (MQ=255)

                        cTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATAc                                                                  <  2:846015/93‑1 (MQ=255)

                         tGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACa                                                                 <  1:10021902/93‑1 (MQ=255)

                         tGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACa                                                                 >  2:887634/1‑93 (MQ=255)

                           gCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAg                                                               <  1:3410820/93‑1 (MQ=255)

                           gCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAg                                                               <  2:4625279/93‑1 (MQ=255)

                           gCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAg                                                               <  2:2884608/93‑1 (MQ=255)

                            ccTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAgg                                                              <  2:91046/93‑1 (MQ=255)

                            ccTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAgg                                                              <  2:4764655/93‑1 (MQ=255)

                            ccTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAgg                                                              <  1:9267278/93‑1 (MQ=255)

                             cTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCGCGCATGGACTGGAACACAAGGt                                                             <  2:7470327/93‑1 (MQ=255)

                             cTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGt                                                             >  2:6065185/1‑93 (MQ=255)

                             cTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGt                                                             <  1:2173847/93‑1 (MQ=255)

                              tCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTg                                                            >  2:9571359/1‑93 (MQ=255)

                              tCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTg                                                            <  2:1828465/93‑1 (MQ=255)

                              tCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTg                                                            <  1:7547164/93‑1 (MQ=255)

                              tCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTg                                                            >  1:2487992/1‑93 (MQ=255)

                               ccGGCGCTAACGAAATGGCGACCGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTgg                                                           >  1:9638585/1‑93 (MQ=255)

                               ccGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTgg                                                           <  2:3643290/93‑1 (MQ=255)

                                cGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGc                                                          <  2:2606007/93‑1 (MQ=255)

                                 ggCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCg                                                         <  1:7242673/93‑1 (MQ=255)

                                   cgcTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTa                                                       <  2:6885631/93‑1 (MQ=255)

                                    gcTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTAt                                                      >  2:4965602/1‑93 (MQ=255)

                                    gcTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTAt                                                      >  2:2954390/1‑93 (MQ=255)

                                    gcTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACCAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTAt                                                      >  2:7014561/1‑93 (MQ=255)

                                     cTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATg                                                     >  2:9507502/1‑93 (MQ=255)

                                      tAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGa                                                    <  1:8274953/93‑1 (MQ=255)

                                      tAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGa                                                    <  2:7533008/93‑1 (MQ=255)

                                      tAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGa                                                    >  1:2717838/1‑93 (MQ=255)

                                         cGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACGAGGTGGCGTATGAAac                                                 >  1:3674519/1‑93 (MQ=255)

                                         cGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAac                                                 <  2:2428895/93‑1 (MQ=255)

                                          gAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAacc                                                <  1:3537713/93‑1 (MQ=255)

                                           aaaTGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAacca                                               <  2:5466553/93‑1 (MQ=255)

                                           aaaTGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAacca                                               <  2:1748342/93‑1 (MQ=255)

                                           aaaTGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAacca                                               <  1:1324494/93‑1 (MQ=255)

                                            aaTGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAaccac                                              >  1:8359828/1‑93 (MQ=255)

                                            aaTGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAaccac                                              <  2:1409927/93‑1 (MQ=255)

                                               ggCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTc                                           >  2:9901688/1‑93 (MQ=255)

                                                  gACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATAGAAGGTGGCGTATGAAACCACCTCGtt                                        <  2:2860129/93‑1 (MQ=255)

                                                   aCAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGttt                                       >  2:7597466/1‑93 (MQ=255)

                                                    cAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTg                                      >  1:3714735/1‑93 (MQ=255)

                                                    cAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTg                                      <  2:6232772/93‑1 (MQ=255)

                                                     aGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGt                                     >  1:4602863/1‑93 (MQ=255)

                                                     aGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGt                                     >  1:8838681/1‑93 (MQ=255)

                                                           gCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGc                               >  2:3591052/1‑93 (MQ=255)

                                                           gCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGc                               <  1:7339887/93‑1 (MQ=255)

                                                           gCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGc                               >  1:984169/1‑93 (MQ=255)

                                                            cTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGcc                              >  1:2337559/1‑93 (MQ=255)

                                                            cTGGTGCTAAATCGTCTCGACGAGCTGGCGCAGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTAGTTTGTTACAGcc                              >  1:4193361/1‑93 (MQ=255)

                                                             tGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCt                             >  1:6996849/1‑93 (MQ=255)

                                                              cgTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTc                            <  1:65819/92‑1 (MQ=255)

                                                                 gCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCAt                         >  1:3044393/1‑93 (MQ=255)

                                                                  cTCAATCGTCTCGACGAGCTGGCGCAGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCAtt                        <  1:5472879/93‑1 (MQ=255)

                                                                   tCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTg                       >  1:8565876/1‑93 (MQ=255)

                                                                   tCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTg                       <  2:3266816/93‑1 (MQ=255)

                                                                    cAATCGTTTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCATCCATTGa                      >  2:2271561/1‑93 (MQ=255)

                                                                     aaTCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGaa                     <  2:92585/93‑1 (MQ=255)

                                                                      aTCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAg                    >  2:9437012/1‑93 (MQ=255)

                                                                      aTCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAg                    <  2:6514272/93‑1 (MQ=255)

                                                                      aTCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAg                    <  1:6916239/93‑1 (MQ=255)

                                                                      aTCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAg                    >  2:3481968/1‑93 (MQ=255)

                                                                       tCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGa                   <  2:1654354/93‑1 (MQ=255)

                                                                       tCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGa                   >  1:6227773/1‑93 (MQ=255)

                                                                       tCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGa                   <  1:5303290/93‑1 (MQ=255)

                                                                       tCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGGTAAAGCCTCCATTGAAGa                   <  2:1183601/93‑1 (MQ=255)

                                                                          tctcGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACgt                >  2:6747401/1‑93 (MQ=255)

                                                                            tcGAGGGGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGCCgtgg              <  2:1926576/93‑1 (MQ=255)

                                                                              gacgaGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACgtggtg            >  1:1569093/1‑93 (MQ=255)

                                                                               acgaGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGa           <  1:566142/93‑1 (MQ=255)

                                                                                cgaGCTGGCGCGGGATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACgtggtgca          >  2:1896119/1‑91 (MQ=255)

                                                                                 gaGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAACCCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGaaa         <  2:6676033/93‑1 (MQ=255)

                                                                                 gaGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGaaa         <  1:7791469/93‑1 (MQ=255)

                                                                                  aGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGaaaa        <  2:8531732/93‑1 (MQ=255)

                                                                                    cTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAAAACg      <  2:2245856/93‑1 (MQ=255)

                                                                                     tGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAAAACgc     <  1:6669150/93‑1 (MQ=255)

                                                                                     tGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAAAACgc     <  1:7852003/93‑1 (MQ=255)

                                                                                     tGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAAAACgc     >  2:5253837/1‑93 (MQ=255)

                                                                                     tGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAAAACgc     >  2:5110885/1‑93 (MQ=255)

                                                                                       gcgcGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAAAACgctg   <  2:9424690/93‑1 (MQ=255)

CCAGCCTCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCAGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAAAACGCTGC  >  NC_000913/2586467‑2586647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCCAGCCTCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCAGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAAAACGCTGCTGGA  >  NC_000913/2586466‑2586651

GCCAGCCTCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGG                                                                                              >  SRR4302540.8275238/1‑94 (MQ=60)

 CCACCCTCCGGGCTGGTGTGAAAACTGGCCTCGGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTC‑ATCGTCTCGACGAGCTGGCGCGGTA                                                                                            <  SRR4302540.5941702/94‑1 (MQ=60)

  CAGCCTCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTA                                                                                            >  SRR4302540.4039767/1‑94 (MQ=60)

   AGCCTCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTAT                                                                                           >  SRR4302540.8566849/1‑94 (MQ=60)

    GCCTCCTGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATT                                                                                          <  SRR4302540.4611844/94‑1 (MQ=60)

    GCCTCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATT                                                                                          <  SRR4302540.472529/94‑1 (MQ=60)

    GCCTCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATT                                                                                          >  SRR4302540.3131993/1‑94 (MQ=60)

    GCCTCCGGGCTGGGGGTAAAACTGGCCGCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATT                                                                                          <  SRR4302540.6104989/94‑1 (MQ=60)

     GCTCCGGGATGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTT                                                                                         <  SRR4302540.8992304/94‑1 (MQ=60)

     CCTCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTTAATCGTCTCGACGAGCTGGCGCGGTATTT                                                                                         >  SRR4302540.5557020/1‑94 (MQ=60)

      CTCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTC                                                                                        >  SRR4302540.1074394/1‑94 (MQ=60)

      CTCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTC                                                                                        >  SRR4302540.6515266/1‑94 (MQ=60)

          GGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGC                                                                                    >  SRR4302540.9654505/1‑94 (MQ=60)

          GGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGC                                                                                    >  SRR4302540.8068432/1‑94 (MQ=60)

           GGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCA                                                                                   >  SRR4302540.9682477/1‑94 (MQ=60)

           GGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCA                                                                                   <  SRR4302540.4405444/94‑1 (MQ=60)

            GCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCAT                                                                                  <  SRR4302540.4554090/94‑1 (MQ=60)

            GCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGGATTTCCCGCAT                                                                                  >  SRR4302540.5552926/1‑94 (MQ=60)

             CTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATG                                                                                 <  SRR4302540.7665412/94‑1 (MQ=60)

              TGGGGGTAAAACTGGCCTGCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGG                                                                                <  SRR4302540.3095916/94‑1 (MQ=60)

              TGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGG                                                                                <  SRR4302540.2321986/94‑1 (MQ=60)

              TGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGG                                                                                <  SRR4302540.9594191/94‑1 (MQ=60)

              TGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGG                                                                                >  SRR4302540.3186495/1‑94 (MQ=60)

              TGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGG                                                                                >  SRR4302540.6719818/1‑94 (MQ=60)

                GGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGAC                                                                              <  SRR4302540.9029841/94‑1 (MQ=60)

                GGGATAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCAGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGAC                                                                              <  SRR4302540.9029842/94‑1 (MQ=60)

                 GGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACT                                                                             <  SRR4302540.5956401/94‑1 (MQ=60)

                 GGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACT                                                                             <  SRR4302540.9500837/94‑1 (MQ=60)

                  GGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTG                                                                            <  SRR4302540.3019248/94‑1 (MQ=60)

                    TAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGA                                                                          <  SRR4302540.9435264/94‑1 (MQ=60)

                      AAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAAT                                                                        >  SRR4302540.9670742/1‑94 (MQ=60)

                      AAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAAT                                                                        >  SRR4302540.3657461/1‑94 (MQ=60)

                      AAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAAT                                                                        >  SRR4302540.9670741/1‑94 (MQ=60)

                       AACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATA                                                                       >  SRR4302540.3633529/1‑94 (MQ=60)

                        ACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATAC                                                                      <  SRR4302540.8109251/94‑1 (MQ=60)

                        ACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATAC                                                                      >  SRR4302540.1363214/1‑94 (MQ=60)

                         CTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACA                                                                     >  SRR4302540.2239414/1‑94 (MQ=60)

                          TGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAA                                                                    <  SRR4302540.10138530/94‑1 (MQ=60)

                          TGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAA                                                                    <  SRR4302540.2583657/94‑1 (MQ=60)

                          TGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAA                                                                    <  SRR4302540.10138529/94‑1 (MQ=60)

                            GCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGG                                                                  <  SRR4302540.3447968/94‑1 (MQ=60)

                             CCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGT                                                                 <  SRR4302540.9378113/94‑1 (MQ=60)

                              CTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTG                                                                >  SRR4302540.7617410/1‑94 (MQ=60)

                              CTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTG                                                                >  SRR4302540.2514789/1‑94 (MQ=60)

                              CTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTG                                                                <  SRR4302540.2197291/94‑1 (MQ=60)

                               TCCGGCGCTAACGAAATGGCGACCGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGG                                                               >  SRR4302540.9752129/1‑94 (MQ=60)

                               TCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGG                                                               >  SRR4302540.1879463/1‑94 (MQ=60)

                               TCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGG                                                               <  SRR4302540.7631750/94‑1 (MQ=60)

                                  GGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGT                                                            <  SRR4302540.7323467/94‑1 (MQ=60)

                                  GGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGT                                                            <  SRR4302540.7250527/94‑1 (MQ=60)

                                    CGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTAT                                                          <  SRR4302540.492090/94‑1 (MQ=60)

                                     GCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATG                                                         >  SRR4302540.3439855/1‑94 (MQ=60)

                                     GCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATG                                                         <  SRR4302540.65061/94‑1 (MQ=60)

                                      CTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGA                                                        >  SRR4302540.2747142/1‑94 (MQ=60)

                                       TAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAA                                                       <  SRR4302540.8371079/94‑1 (MQ=60)

                                        AACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAA                                                      <  SRR4302540.4233042/94‑1 (MQ=60)

                                         ACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACGAGGTGGCGTATGAAAC                                                     >  SRR4302540.3714698/1‑94 (MQ=60)

                                         ACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAAC                                                     >  SRR4302540.3714695/1‑94 (MQ=60)

                                           GAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCA                                                   <  SRR4302540.4643890/94‑1 (MQ=60)

                                           GAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCA                                                   >  SRR4302540.7390763/1‑94 (MQ=60)

                                           GAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCA                                                   <  SRR4302540.4541883/94‑1 (MQ=60)

                                           GAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCA                                                   <  SRR4302540.3576339/94‑1 (MQ=60)

                                            gaaagcgggccaCGGAGGTGGTGCTCAATCGTCTCCACGAGCTGGCGCGGTATTTCCCCCATGGACTGGAATACAAGGTGGCGTATGAAACCAC                                                  <  SRR4302540.1338310/82‑1 (MQ=60)

                                            AAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCAC                                                  <  SRR4302540.1338309/94‑1 (MQ=60)

                                            AAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCAC                                                  <  SRR4302540.6613894/94‑1 (MQ=60)

                                            AAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCAC                                                  >  SRR4302540.8456840/1‑94 (MQ=60)

                                               GGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTC                                               <  SRR4302540.8345016/94‑1 (MQ=60)

                                                  CGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTT                                            >  SRR4302540.1298467/1‑94 (MQ=60)

                                                   GACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTT                                           >  SRR4302540.9902913/1‑94 (MQ=60)

                                                   GACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTT                                           >  SRR4302540.8757218/1‑94 (MQ=60)

                                                    ACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTG                                          >  SRR4302540.3755270/1‑94 (MQ=60)

                                                     CAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGT                                         >  SRR4302540.4652317/1‑94 (MQ=60)

                                                     CAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGT                                         >  SRR4302540.8940911/1‑94 (MQ=60)

                                                          GAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAG                                    <  SRR4302540.427266/94‑1 (MQ=60)

                                                           AGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGC                                   >  SRR4302540.994235/1‑94 (MQ=60)

                                                           AGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGC                                   >  SRR4302540.6972993/1‑94 (MQ=60)

                                                            GCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCC                                  >  SRR4302540.2362750/1‑94 (MQ=60)

                                                            GCTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCC                                  <  SRR4302540.7421793/94‑1 (MQ=60)

                                                            GCTGGTGCTAAATCGTCTCGACGAGCTGGCGCAGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTAGTTTGTTACAGCC                                  >  SRR4302540.4238979/1‑94 (MQ=60)

                                                             CTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCT                                 >  SRR4302540.994966/1‑94 (MQ=60)

                                                             CTGGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCT                                 >  SRR4302540.7074508/1‑94 (MQ=60)

                                                               CGTGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCC                               <  SRR4302540.66485/94‑1 (MQ=60)

                                                                 TGCTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCAT                             >  SRR4302540.3077800/1‑94 (MQ=60)

                                                                   CTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTG                           >  SRR4302540.3728805/1‑94 (MQ=60)

                                                                   CTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTG                           <  SRR4302540.6206917/94‑1 (MQ=60)

                                                                   CTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTG                           >  SRR4302540.8665351/1‑94 (MQ=60)

                                                                   CTCAATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTG                           >  SRR4302540.8520052/1‑94 (MQ=60)

                                                                   CTCAATCGTCTCGACGAGCTGGCGCAGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTG                           <  SRR4302540.5533119/94‑1 (MQ=60)

                                                                      AATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAG                        <  SRR4302540.701572/94‑1 (MQ=60)

                                                                      AATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAG                        >  SRR4302540.3466310/1‑94 (MQ=60)

                                                                       ATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGA                       <  SRR4302540.6993197/94‑1 (MQ=60)

                                                                       ATCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGA                       >  SRR4302540.6297036/1‑94 (MQ=60)

                                                                        TCGTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGAC                      <  SRR4302540.5361313/94‑1 (MQ=60)

                                                                          GTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGT                    >  SRR4302540.2502797/1‑94 (MQ=60)

                                                                          GTCTCGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGT                    >  SRR4302540.5640915/1‑94 (MQ=60)

                                                                              CGACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTG                >  SRR4302540.1586296/1‑94 (MQ=60)

                                                                               GACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGA               <  SRR4302540.5518333/94‑1 (MQ=60)

                                                                                ACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAA              <  SRR4302540.6850830/94‑1 (MQ=60)

                                                                                ACGAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAA              <  SRR4302540.572298/94‑1 (MQ=60)

                                                                                  GAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAAAA            <  SRR4302540.7882546/94‑1 (MQ=60)

                                                                                  GAGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAAAA            >  SRR4302540.8442535/1‑94 (MQ=60)

                                                                                   AGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAAAAC           <  SRR4302540.4596466/94‑1 (MQ=60)

                                                                                   AGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAAAAC           <  SRR4302540.9432008/94‑1 (MQ=60)

                                                                                   AGCTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGGATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAAAAC           >  SRR4302540.7696977/1‑94 (MQ=60)

                                                                                     CTGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAAAACGC         >  SRR4302540.9359546/1‑94 (MQ=60)

                                                                                      TGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAAAACGCT        <  SRR4302540.7943751/94‑1 (MQ=60)

                                                                                      TGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAAAACGCT        <  SRR4302540.7943750/94‑1 (MQ=60)

                                                                                      TGGCGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAAAACGCT        <  SRR4302540.6742854/94‑1 (MQ=60)

                                                                                         CGCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAAAACGCTGCT     >  SRR4302540.5222833/1‑94 (MQ=60)

                                                                                          GCGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAAAACGCTGCTG    >  SRR4302540.2471586/1‑94 (MQ=60)

                                                                                           CGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGAGGTGGTGAAAACGCTGCTGG   <  SRR4302540.1989639/94‑1 (MQ=60)

                                                                                           CGGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAAAACGCTGCTGG   >  SRR4302540.3440651/1‑94 (MQ=60)

                                                                                            GGTATTTCCCGCGTGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAAAACGCTGCTGGA  <  SRR4302540.2843100/94‑1 (MQ=60)

                                                                                            GGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAAAACGCTGCTGGA  <  SRR4302540.19717/94‑1 (MQ=60)

                                                                                            GGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAAAACGCTGCTGGA  >  SRR4302540.6794151/1‑94 (MQ=60)

                                                                                            GGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAAAACGCTGCTGGA  >  SRR4302540.6853295/1‑94 (MQ=60)

GCCAGCCTCCGGGCTGGGGGTAAAACTGGCCTCCGGCGCTAACGAAATGGCGACAGCGGAGCTGGTGCTCAATCGTCTCGACGAGCTGGCGCAGTATTTCCCGCATGGACTGGAATACAAGGTGGCGTATGAAACCACCTCGTTTGTTAAAGCCTCCATTGAAGACGTGGTGAAAACGCTGCTGGA  >  NC_000913/2586466‑2586651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`