Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,690,602 T→C T988A (ACT→GCT)  purL ← phosphoribosylformyl‑glycineamide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,690,6020TC100.0% 743.3 / NA 225T988A (ACT→GCT) purLphosphoribosylformyl‑glycineamide synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (115/110);  total (115/110)

Only 100 of 230 total aligned reads displayed.

CAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCG  >  NC_000913/2690513‑2690691
                                                                                         |                                                                                         
cAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCCGGTAGCTTc                                                                                        >  1:8729609/1‑93 (MQ=255)
cAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTc                                                                                        <  2:5465939/93‑1 (MQ=255)
cAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTc                                                                                        <  2:1754186/93‑1 (MQ=255)
 aGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCt                                                                                       <  2:9863397/93‑1 (MQ=255)
   gCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGc                                                                                     <  1:5182042/93‑1 (MQ=255)
      cGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCcca                                                                                  <  1:5682241/93‑1 (MQ=255)
      cGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCcca                                                                                  >  2:1406080/1‑93 (MQ=255)
       ggATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCTGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTCTccac                                                                                 >  2:3899809/1‑93 (MQ=255)
        gATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCccacc                                                                                >  2:7958518/1‑93 (MQ=255)
         gtCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCccacca                                                                               <  1:2506063/92‑1 (MQ=255)
          tCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAg                                                                              >  2:1028222/1‑93 (MQ=255)
          tCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAg                                                                              <  2:311156/93‑1 (MQ=255)
          tCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAg                                                                              >  2:6536249/1‑93 (MQ=255)
           cGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGa                                                                             >  2:6154191/1‑93 (MQ=255)
            ggCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGac                                                                            <  1:1604483/93‑1 (MQ=255)
             gCGTCGTTAGATTTCGCCTGATGCTCCTTATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGaca                                                                           >  2:6310674/1‑93 (MQ=255)
             gCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGaca                                                                           >  2:3525513/1‑93 (MQ=255)
             gCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGaca                                                                           <  1:7771779/93‑1 (MQ=255)
             gCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGaca                                                                           <  2:4715344/93‑1 (MQ=255)
               gtcgtTAGATTTCGCCTGATGCCCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACg                                                                         >  2:9357457/1‑93 (MQ=255)
                tcgtTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGc                                                                        >  2:326333/1‑93 (MQ=255)
                 cgtTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCa                                                                       >  2:8291599/1‑93 (MQ=255)
                 cgtTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCa                                                                       <  2:9116187/93‑1 (MQ=255)
                  gtTAGATTTCGCCTGATGCTCCTGATCGGCCCACTCCGGGTTGTCACGCAGGAGCTGCCTCTGCCAGGGCGGTTCTGCCCACCAGAAACGCaa                                                                      >  1:4106671/1‑93 (MQ=255)
                   ttAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCATGTAGCTTCTGCCCACCAGACACGCAAc                                                                     >  1:221139/1‑93 (MQ=255)
                   ttAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAAc                                                                     <  1:8378195/93‑1 (MQ=255)
                   ttAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAAc                                                                     >  2:2443260/1‑93 (MQ=255)
                     aGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACgt                                                                   >  1:3937576/1‑93 (MQ=255)
                      gATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACgtg                                                                  <  2:6261481/93‑1 (MQ=255)
                       aTTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACgtgg                                                                 <  1:4583762/93‑1 (MQ=255)
                       aTTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACgtgg                                                                 >  1:3688029/1‑93 (MQ=255)
                       aTTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACgtgg                                                                 >  2:4026478/1‑93 (MQ=255)
                        tttCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACgtggt                                                                >  2:3929351/1‑93 (MQ=255)
                        tttCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACgtggt                                                                >  2:9316927/1‑93 (MQ=255)
                         ttCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACgtggtg                                                               <  1:5118976/93‑1 (MQ=255)
                            gCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCgg                                                            >  2:1170136/1‑93 (MQ=255)
                             ccTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGc                                                           <  1:2558176/93‑1 (MQ=255)
                              cTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCt                                                          >  2:1991019/1‑93 (MQ=255)
                              cTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCt                                                          <  1:3032249/93‑1 (MQ=255)
                              cTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCt                                                          >  1:9578762/1‑93 (MQ=255)
                              cTGATGCTCCTGATCGGCACACTCCGGGGTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCt                                                          >  1:5591932/1‑93 (MQ=255)
                               tGATGCTCCTGATCGGCACCCTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCtt                                                         <  1:3125205/93‑1 (MQ=255)
                               tGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCtt                                                         >  1:6340167/1‑93 (MQ=255)
                               tGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCtt                                                         >  1:7576154/1‑93 (MQ=255)
                                gATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCttt                                                        <  1:2678755/93‑1 (MQ=255)
                                 aTGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTc                                                       <  1:8759498/93‑1 (MQ=255)
                                  tGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCg                                                      <  2:3653466/93‑1 (MQ=255)
                                  ggCTCCTGATCGGCACGCTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCg                                                      <  1:733979/92‑1 (MQ=255)
                                   gCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGc                                                     <  1:1588398/93‑1 (MQ=255)
                                    cTCCTGATCGGCACGCTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCt                                                    <  1:1929043/93‑1 (MQ=255)
                                    cTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCt                                                    <  1:7631282/93‑1 (MQ=255)
                                    cTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCt                                                    >  1:4796386/1‑93 (MQ=255)
                                      ccTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGa                                                  <  1:6516490/93‑1 (MQ=255)
                                       cTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGaa                                                 >  1:5863128/1‑93 (MQ=255)
                                       cTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGaa                                                 >  1:2376163/1‑93 (MQ=255)
                                        tGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAAt                                                >  2:4601427/1‑93 (MQ=255)
                                        tGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAAt                                                <  1:6781607/93‑1 (MQ=255)
                                         gATCGGCACACGCCGGGTTGTCACGCAGGCTCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATa                                               <  1:4948151/93‑1 (MQ=255)
                                          aTCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATAc                                              >  2:7064602/1‑93 (MQ=255)
                                          aTCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATAc                                              <  2:3158143/93‑1 (MQ=255)
                                           gcGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACa                                             <  1:2768216/92‑1 (MQ=255)
                                             ggCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGt                                           <  1:9226214/93‑1 (MQ=255)
                                             ggCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGt                                           <  1:3653041/93‑1 (MQ=255)
                                             ggCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGt                                           <  2:7733540/93‑1 (MQ=255)
                                              gCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTc                                          <  2:8726413/93‑1 (MQ=255)
                                              gCACACTCCGGGTTGTCACACAGGCGCTGCATCAGCCAGGTCGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTc                                          <  1:8963217/93‑1 (MQ=255)
                                               cacaCTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCt                                         >  1:3556237/1‑93 (MQ=255)
                                               cacaCTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCt                                         <  1:819289/93‑1 (MQ=255)
                                                 cacTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGc                                       >  2:5643148/1‑93 (MQ=255)
                                                   cTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGccc                                     <  1:5342860/93‑1 (MQ=255)
                                                   cTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGccc                                     >  1:7695077/1‑93 (MQ=255)
                                                      cGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGtt                                  <  2:4550436/93‑1 (MQ=255)
                                                      cGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGtt                                  <  1:6294737/93‑1 (MQ=255)
                                                      cGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGtt                                  <  1:2907462/93‑1 (MQ=255)
                                                       gggTTGTCACGCAGACGCTGCATCTGCAAGGTAGCTTCTGCCCACCAGACCCGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTg                                 <  2:864972/93‑1 (MQ=255)
                                                         gTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGc                               >  1:5696364/1‑93 (MQ=255)
                                                          ttGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCg                              >  2:8341207/1‑93 (MQ=255)
                                                          ttGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCg                              >  2:9552881/1‑93 (MQ=255)
                                                          ttGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCg                              >  1:3773418/1‑93 (MQ=255)
                                                            gTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGt                            >  1:695764/1‑93 (MQ=255)
                                                              cACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTaa                          <  2:5840661/93‑1 (MQ=255)
                                                                ggaaGGCGCTGCATCTGCTAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGACTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATc                        <  2:6855400/90‑1 (MQ=255)
                                                                cGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATc                        <  1:4352461/93‑1 (MQ=255)
                                                                  cAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATcac                      <  2:8788984/93‑1 (MQ=255)
                                                                    ggCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACaa                    <  2:8618538/93‑1 (MQ=255)
                                                                     gcgcTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACaaa                   <  2:5066275/93‑1 (MQ=255)
                                                                      cgcTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTTAATACAGTCTGCCCGTTGGCGGTAATCACaaaa                  <  2:8172467/93‑1 (MQ=255)
                                                                        cTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACg                >  2:6599829/1‑93 (MQ=255)
                                                                         tGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACgg               <  2:6915046/93‑1 (MQ=255)
                                                                         tGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACgg               >  2:6667895/1‑93 (MQ=255)
                                                                         tGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACgg               <  2:1640058/93‑1 (MQ=255)
                                                                          gCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGt              >  2:9917909/1‑93 (MQ=255)
                                                                          gCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGt              <  2:1966599/93‑1 (MQ=255)
                                                                             tCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCAc           <  1:1864784/93‑1 (MQ=255)
                                                                             tCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCAc           <  1:8652726/93‑1 (MQ=255)
                                                                                gCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCgg        >  2:3392530/1‑93 (MQ=255)
                                                                                   aGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGaaa     >  2:695648/1‑93 (MQ=255)
                                                                                    ggTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAAc    >  1:107310/1‑93 (MQ=255)
                                                                                      tAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCg  >  1:7101317/1‑93 (MQ=255)
                                                                                      tAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCg  <  2:8011620/93‑1 (MQ=255)
                                                                                         |                                                                                         
CAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCG  >  NC_000913/2690513‑2690691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAAC‑GGTCACCGGAAACCGCCT  >  NC_000913/2690510‑2690694
                                                                                            |                                                                                             
ATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCT                                                                                              >  SRR4302540.7101004/1‑94 (MQ=60)
  TCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCCGGTAGCTTC                                                                                            >  SRR4302540.8830981/1‑94 (MQ=60)
  TCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTC                                                                                            >  SRR4302540.8830988/1‑94 (MQ=60)
     GGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGC                                                                                         >  SRR4302540.173621/1‑94 (MQ=60)
      GCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCC                                                                                        <  SRR4302540.5238451/94‑1 (MQ=60)
       CCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCAGGCAGGCGCTGCCTCTGCCAGGTAGCTTCTGCCC                                                                                       >  SRR4302540.8772722/1‑94 (MQ=60)
       CCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCC                                                                                       >  SRR4302540.345100/1‑94 (MQ=60)
       CCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCC                                                                                       >  SRR4302540.2500903/1‑94 (MQ=60)
        CCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCA                                                                                      >  SRR4302540.4855383/1‑94 (MQ=60)
         CGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCGAC                                                                                     >  SRR4302540.6915599/1‑94 (MQ=60)
         CGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCAC                                                                                     <  SRR4302540.5744801/94‑1 (MQ=60)
         CGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCACCTGCCAGGTAGCTTCTGCCCAC                                                                                     >  SRR4302540.7847992/1‑94 (MQ=60)
          GGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACC                                                                                    >  SRR4302540.1926854/1‑94 (MQ=60)
          GGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACC                                                                                    >  SRR4302540.5808144/1‑94 (MQ=60)
          GGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACC                                                                                    >  SRR4302540.811619/1‑94 (MQ=60)
           GATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCA                                                                                   <  SRR4302540.1957468/94‑1 (MQ=60)
            GTCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAG                                                                                  <  SRR4302540.2533120/94‑1 (MQ=60)
             TCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGA                                                                                 >  SRR4302540.1611741/1‑94 (MQ=60)
              CGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGAC                                                                                <  SRR4302540.5138082/94‑1 (MQ=60)
              CGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGAC                                                                                >  SRR4302540.1005159/1‑94 (MQ=60)
               GGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACA                                                                               <  SRR4302540.1622039/94‑1 (MQ=60)
                GCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACAC                                                                              <  SRR4302540.2829556/94‑1 (MQ=60)
                GCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACAC                                                                              <  SRR4302540.7862637/94‑1 (MQ=60)
                  GTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGC                                                                            >  SRR4302540.5489670/1‑94 (MQ=60)
                  GTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGC                                                                            >  SRR4302540.3005237/1‑94 (MQ=60)
                  GTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGC                                                                            >  SRR4302540.4600827/1‑94 (MQ=60)
                   TCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCA                                                                           >  SRR4302540.8873347/1‑94 (MQ=60)
                    CGTTAGATTTCGCCTGATGCTCCTGATCGGCCCACTCCGGGTTGTCACGCAGGAGCTGCCTCTGCCAGGGCGGTTCTGCCCACCAGAAACGCAA                                                                          >  SRR4302540.4150976/1‑94 (MQ=60)
                    CGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAA                                                                          >  SRR4302540.8890679/1‑94 (MQ=60)
                    CGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAA                                                                          >  SRR4302540.4151304/1‑94 (MQ=60)
                     GTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCATGTAGCTTCTGCCCACCAGACACGCAAC                                                                         >  SRR4302540.223284/1‑94 (MQ=60)
                     GTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAAC                                                                         <  SRR4302540.6678469/94‑1 (MQ=60)
                     GTTAGATTTCCCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAAC                                                                         <  SRR4302540.2789491/94‑1 (MQ=60)
                      TTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACG                                                                        <  SRR4302540.8475418/94‑1 (MQ=60)
                       TAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGT                                                                       >  SRR4302540.3980122/1‑94 (MQ=60)
                         GATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGG                                                                     <  SRR4302540.3134196/94‑1 (MQ=60)
                         GATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGG                                                                     >  SRR4302540.3728325/1‑94 (MQ=60)
                          ATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGT                                                                    <  SRR4302540.4633156/94‑1 (MQ=60)
                          ATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGT                                                                    <  SRR4302540.7700463/94‑1 (MQ=60)
                          ATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGT                                                                    <  SRR4302540.3813488/94‑1 (MQ=60)
                            TTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGC                                                                  <  SRR4302540.5174398/94‑1 (MQ=60)
                                CCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCT                                                              >  SRR4302540.9691884/1‑94 (MQ=60)
                                CCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCT                                                              <  SRR4302540.2585746/94‑1 (MQ=60)
                                CCTGATGCTCCTGATCGGCACACTCCGGGGTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCT                                                              >  SRR4302540.5653462/1‑94 (MQ=60)
                                 CTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTT                                                             <  SRR4302540.7262/94‑1 (MQ=60)
                                 CTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTT                                                             >  SRR4302540.7661163/1‑94 (MQ=60)
                                 CTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTT                                                             >  SRR4302540.7749278/1‑94 (MQ=60)
                                 CTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTT                                                             >  SRR4302540.6410775/1‑94 (MQ=60)
                                 CTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTT                                                             <  SRR4302540.3065540/94‑1 (MQ=60)
                                  TGATGCTCCTGATCGGCACCCTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTT                                                            <  SRR4302540.3159335/94‑1 (MQ=60)
                                   GATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTC                                                           >  SRR4302540.5791669/1‑94 (MQ=60)
                                   GATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTC                                                           <  SRR4302540.2707739/94‑1 (MQ=60)
                                   GATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTC                                                           <  SRR4302540.261613/94‑1 (MQ=60)
                                    ATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCG                                                          <  SRR4302540.8861075/94‑1 (MQ=60)
                                    ATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCG                                                          >  SRR4302540.8542661/1‑94 (MQ=60)
                                    ATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCG                                                          <  SRR4302540.2463815/94‑1 (MQ=60)
                                     GGCTCCTGATCGGCACGCTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGC                                                         <  SRR4302540.741829/94‑1 (MQ=60)
                                      GCTCCTGATCGGCACACTCCGGGTTGTCACGCCGGCGCTGCATCTGCCTGGTAGCTTCTGCCCACCAGACACGCAACGTGGAGCGGCTGTCGCG                                                        >  SRR4302540.4847714/1‑94 (MQ=60)
                                      GCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCT                                                        <  SRR4302540.1605752/94‑1 (MQ=60)
                                      GCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCT                                                        >  SRR4302540.4847715/1‑94 (MQ=60)
                                       CTCCTGATCGGCACGCTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTG                                                       <  SRR4302540.1950068/94‑1 (MQ=60)
                                       CTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTG                                                       <  SRR4302540.7717122/94‑1 (MQ=60)
                                         CCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAA                                                     <  SRR4302540.5591579/94‑1 (MQ=60)
                                         CCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAA                                                     >  SRR4302540.5927956/1‑94 (MQ=60)
                                         CCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAA                                                     <  SRR4302540.6588882/94‑1 (MQ=60)
                                         CCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAA                                                     >  SRR4302540.4772872/1‑94 (MQ=60)
                                         CCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAA                                                     >  SRR4302540.2401774/1‑94 (MQ=60)
                                           TGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATA                                                   >  SRR4302540.3971573/1‑94 (MQ=60)
                                           TGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATA                                                   <  SRR4302540.6856593/94‑1 (MQ=60)
                                           TGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGACCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATA                                                   >  SRR4302540.3796734/1‑94 (MQ=60)
                                            GATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATAC                                                  <  SRR4302540.1331312/94‑1 (MQ=60)
                                            GATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATAC                                                  <  SRR4302540.5001591/94‑1 (MQ=60)
                                            GATCGGCACACGCCGGGTTGTCACGCAGGCTCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATAC                                                  <  SRR4302540.5001590/94‑1 (MQ=60)
                                             ATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACA                                                 <  SRR4302540.9591467/94‑1 (MQ=60)
                                              TCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTCCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAG                                                <  SRR4302540.2798134/94‑1 (MQ=60)
                                              GCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAG                                                <  SRR4302540.2798135/94‑1 (MQ=60)
                                                GGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTC                                              <  SRR4302540.9336835/94‑1 (MQ=60)
                                                GGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTC                                              <  SRR4302540.231958/94‑1 (MQ=60)
                                                GGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTC                                              <  SRR4302540.3693051/94‑1 (MQ=60)
                                                GGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTC                                              <  SRR4302540.6618680/94‑1 (MQ=60)
                                                 GCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCT                                             <  SRR4302540.5185918/94‑1 (MQ=60)
                                                 GCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCT                                             >  SRR4302540.3595095/1‑94 (MQ=60)
                                                 GCACACTCCGGGTTGTCACACAGGCGCTGCATCAGCCAGGTCGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCT                                             <  SRR4302540.9069343/94‑1 (MQ=60)
                                                  CACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTG                                            <  SRR4302540.827998/94‑1 (MQ=60)
                                                  CACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTG                                            <  SRR4302540.741503/94‑1 (MQ=60)
                                                    CACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCC                                          <  SRR4302540.8356246/94‑1 (MQ=60)
                                                     ACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCC                                         <  SRR4302540.3622653/94‑1 (MQ=60)
                                                     ACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCC                                         >  SRR4302540.7782730/1‑94 (MQ=60)
                                                     ACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCC                                         >  SRR4302540.7782729/1‑94 (MQ=60)
                                                     ACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCC                                         <  SRR4302540.7167593/94‑1 (MQ=60)
                                                      CTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCG                                        <  SRR4302540.5401461/94‑1 (MQ=60)
                                                        CCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTT                                      <  SRR4302540.6470715/94‑1 (MQ=60)
                                                         CGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTG                                     <  SRR4302540.2939214/94‑1 (MQ=60)
                                                         CGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTG                                     <  SRR4302540.6364823/94‑1 (MQ=60)
                                                           GGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGC                                   >  SRR4302540.5759081/1‑94 (MQ=60)
                                                           GGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGC                                   <  SRR4302540.1897946/94‑1 (MQ=60)
                                                           GGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGC                                   >  SRR4302540.5759082/1‑94 (MQ=60)
                                                            GTTGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCG                                  >  SRR4302540.3814591/1‑94 (MQ=60)
                                                              TGTCACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGT                                >  SRR4302540.703228/1‑94 (MQ=60)
                                                                 CACGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAAT                             >  SRR4302540.9163086/1‑94 (MQ=60)
                                                                   CGCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCA                           <  SRR4302540.4399804/94‑1 (MQ=60)
                                                                    GCAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCAC                          >  SRR4302540.6061205/1‑94 (MQ=60)
                                                                     CAGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACA                         >  SRR4302540.4099119/1‑94 (MQ=60)
                                                                      AGGCGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAA                        <  SRR4302540.8673583/94‑1 (MQ=60)
                                                                         CGCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAAC                     >  SRR4302540.5251171/1‑94 (MQ=60)
                                                                          GCTGCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAAC‑G                   >  SRR4302540.2544831/1‑94 (MQ=60)
                                                                             GCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAAC‑GGTC                <  SRR4302540.7450742/94‑1 (MQ=60)
                                                                             GCATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAAC‑GGTC                <  SRR4302540.4386917/94‑1 (MQ=60)
                                                                              CATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAAC‑GGTCA               <  SRR4302540.9966514/94‑1 (MQ=60)
                                                                               ATCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAAC‑GGTCAC              <  SRR4302540.5680396/94‑1 (MQ=60)
                                                                                TCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAAC‑GGTCACC             <  SRR4302540.1885226/94‑1 (MQ=60)
                                                                                TCTGCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAAC‑GGTCACC             <  SRR4302540.8753241/94‑1 (MQ=60)
                                                                                   GCCAGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAAC‑GGTCACCGGA          <  SRR4302540.3422468/94‑1 (MQ=60)
                                                                                      AGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCGGTT‑GCGGGAAACACAAAACGGGTAACCGGAAAC       >  SRR4302540.6409358/1‑94 (MQ=60)
                                                                                      AGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAAC‑GGTCACCGGAAAC       >  SRR4302540.108460/1‑94 (MQ=60)
                                                                                      AGGTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAAC‑GGTCACCGGAAAC       <  SRR4302540.3727067/94‑1 (MQ=60)
                                                                                        GTAGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAAC‑GGTCACCGGAAACCG     >  SRR4302540.7180331/1‑94 (MQ=60)
                                                                                          AGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAAC‑GGTCACCGGAAACCGCC   <  SRR4302540.8630477/94‑1 (MQ=60)
                                                                                          AGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAAC‑GGTCACCGGAAACCGCC   >  SRR4302540.6883275/1‑94 (MQ=60)
                                                                                          AGCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAAC‑GGTCACCGGAAACCGCC   <  SRR4302540.1326477/94‑1 (MQ=60)
                                                                                           GCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAAC‑GGTCACCGGAAACCGCCT  <  SRR4302540.8699990/94‑1 (MQ=60)
                                                                                           GCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAAC‑GGTCACCGGAAACCGCCT  >  SRR4302540.5710642/1‑94 (MQ=60)
                                                                                           GCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAAC‑GGTCACCGGAAACCGCCT  <  SRR4302540.9428110/94‑1 (MQ=60)
                                                                                           GCTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTCCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAAC‑GGTCACCGGAAACCGCCT  <  SRR4302540.5331469/94‑1 (MQ=60)
                                                                                            |                                                                                             
ATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAAC‑GGTCACCGGAAACCGCCT  >  NC_000913/2690510‑2690694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑