Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,691,024 A→T F847Y (TTT→TAT)  purL ← phosphoribosylformyl‑glycineamide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,691,0240AT99.7% 1047.2 / NA 285F847Y (TTT→TAT) purLphosphoribosylformyl‑glycineamide synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (134/150);  total (134/151)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 296 total aligned reads displayed.

CGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGAAAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGC  >  NC_000913/2690934‑2691113
                                                                                          |                                                                                         
 gCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGataaa                                                                                        <  2:3084988/93‑1 (MQ=255)
 gCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGataaa                                                                                        >  1:9721206/1‑93 (MQ=255)
   tgtgCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAAcc                                                                                      >  1:5498676/1‑93 (MQ=255)
   tgtgCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAAcc                                                                                      <  1:6855186/93‑1 (MQ=255)
    gtgCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCt                                                                                     <  1:3416200/93‑1 (MQ=255)
     tgCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTg                                                                                    >  2:4543106/1‑93 (MQ=255)
      gCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTgc                                                                                   >  2:1374643/1‑93 (MQ=255)
      gCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTgc                                                                                   <  2:6202875/93‑1 (MQ=255)
          ccAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCa                                                                               <  1:3198070/93‑1 (MQ=255)
            aGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTTGCCAAGCTGACGATAAACCTGCGCCatc                                                                             >  1:7215542/1‑91 (MQ=255)
            aGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAgc                                                                             >  2:9704254/1‑93 (MQ=255)
            aGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAgc                                                                             >  1:7105816/1‑93 (MQ=255)
             gcgcCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAgcg                                                                            >  2:6286240/1‑93 (MQ=255)
             gcgcCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAgcg                                                                            <  1:3720495/93‑1 (MQ=255)
              cgcCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAgcgc                                                                           <  2:4470786/93‑1 (MQ=255)
              cgcCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAgcgc                                                                           <  2:2972493/93‑1 (MQ=255)
                ccTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCg                                                                         <  2:1017425/93‑1 (MQ=255)
                 cTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGt                                                                        <  2:8787928/93‑1 (MQ=255)
                  tGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGtt                                                                       >  2:3412602/1‑93 (MQ=255)
                  tGAATCGCGGCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGTTGGGTCCCCAAGCTGACGATAAACCTGCGGCAGCGCCGtt                                                                       <  2:8630406/93‑1 (MQ=255)
                   gAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTg                                                                      <  2:5572462/93‑1 (MQ=255)
                     aTCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTgcg                                                                    >  2:6061727/1‑93 (MQ=255)
                     aTCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTgcg                                                                    >  2:4828324/1‑93 (MQ=255)
                      tCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCACGCTGACGATAAACCTGCGCCCACCCCGTTgcgc                                                                   >  2:1878817/1‑93 (MQ=255)
                      tCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTgcgc                                                                   >  2:6567927/1‑93 (MQ=255)
                         cgTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCa                                                                <  2:2240481/93‑1 (MQ=255)
                           tCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAgc                                                              >  2:8055482/1‑93 (MQ=255)
                            cATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAgcg                                                             >  2:5236599/1‑93 (MQ=255)
                             aTAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAgcgc                                                            >  1:8407026/1‑93 (MQ=255)
                              tAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGcgcg                                                           >  2:66159/1‑93 (MQ=255)
                              tAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGcgcg                                                           >  2:7537171/1‑93 (MQ=255)
                               aGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGt                                                          >  1:6754462/1‑93 (MQ=255)
                                 aaGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTa                                                        <  1:5574730/93‑1 (MQ=255)
                                  aGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTAt                                                       >  1:6524885/1‑93 (MQ=255)
                                  aGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTAt                                                       <  1:391035/93‑1 (MQ=255)
                                  aGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTAt                                                       <  2:2546272/93‑1 (MQ=255)
                                  aGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTAt                                                       <  1:2218890/93‑1 (MQ=255)
                                  aGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGATTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTAt                                                       <  2:6894172/93‑1 (MQ=255)
                                   gCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTact                                                      >  1:8242615/1‑91 (MQ=255)
                                    ccTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTCTTg                                                     >  1:9211259/1‑93 (MQ=255)
                                      tttCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGcc                                                   >  1:4969514/1‑93 (MQ=255)
                                        tCAGTTGCGCGACATCGGGTACATCTGCCGGTTTGGCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCATTATTGCCtt                                                 <  1:2659002/93‑1 (MQ=255)
                                        tCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCtt                                                 <  2:3709093/93‑1 (MQ=255)
                                        tCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCtt                                                 <  1:5964732/93‑1 (MQ=255)
                                         cAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCttt                                                <  2:6385702/93‑1 (MQ=255)
                                          aGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTg                                               <  1:208488/93‑1 (MQ=255)
                                          aGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTg                                               <  2:476879/93‑1 (MQ=255)
                                            ttGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGcc                                             <  2:729799/93‑1 (MQ=255)
                                            ttGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGcc                                             <  2:1626028/93‑1 (MQ=255)
                                                gcgACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCaaa                                         <  1:7055231/93‑1 (MQ=255)
                                                gcgACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCaaa                                         >  2:8848492/1‑93 (MQ=255)
                                                 cgACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaat                                        <  2:1668060/93‑1 (MQ=255)
                                                 cgACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaat                                        <  1:612431/93‑1 (MQ=255)
                                                   aCATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatca                                      >  2:760210/1‑93 (MQ=255)
                                                      tCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatcaatc                                   >  2:5456046/1‑93 (MQ=255)
                                                      tCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatcaatc                                   <  2:857439/93‑1 (MQ=255)
                                                      tCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatcaatc                                   <  1:2354817/93‑1 (MQ=255)
                                                      tCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatcaatc                                   <  1:2242728/93‑1 (MQ=255)
                                                      tCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatcaatc                                   >  1:9493246/1‑93 (MQ=255)
                                                      tCGCGTACATCTGCCGGTTGGTCGCCAAGCGGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTGATTGCCTTGGCCCAaatcaatc                                   <  1:4802673/93‑1 (MQ=255)
                                                       cgcgTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAatcaatca                                  <  1:287102/93‑1 (MQ=255)
                                                         cgTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATcagc                                <  2:4413611/93‑1 (MQ=255)
                                                         cgTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATcagc                                <  2:4182556/93‑1 (MQ=255)
                                                         cgTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATcagc                                >  2:9930676/1‑93 (MQ=255)
                                                         cgTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATcagc                                <  1:586486/93‑1 (MQ=255)
                                                         cgTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATcagc                                >  1:4705860/1‑93 (MQ=255)
                                                         cgTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATcagc                                <  1:4316282/93‑1 (MQ=255)
                                                         cgTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATcagc                                >  1:9043752/1‑93 (MQ=255)
                                                          gTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATcagca                               >  2:6712178/1‑93 (MQ=255)
                                                             cATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATcagcagca                            <  2:4050046/93‑1 (MQ=255)
                                                             cATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATcagcagca                            <  2:5988297/93‑1 (MQ=255)
                                                             cATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATcagcagca                            <  2:3184311/93‑1 (MQ=255)
                                                               tCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGt                          <  1:2496508/93‑1 (MQ=255)
                                                               tCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGt                          <  2:8923876/93‑1 (MQ=255)
                                                                  gCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCg                       >  2:8287395/1‑93 (MQ=255)
                                                                  gCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCg                       <  1:1611710/93‑1 (MQ=255)
                                                                    cGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGtt                     <  1:8025407/93‑1 (MQ=255)
                                                                     ggTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTa                    <  2:5516766/93‑1 (MQ=255)
                                                                     ggTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTa                    <  1:8607841/93‑1 (MQ=255)
                                                                     ggTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTa                    <  1:1121271/93‑1 (MQ=255)
                                                                       tttGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATc                  <  1:1990321/93‑1 (MQ=255)
                                                                         tGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCtt                <  1:1011340/93‑1 (MQ=255)
                                                                         tGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCtt                <  1:6959471/93‑1 (MQ=255)
                                                                          gTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTc               >  2:2795693/1‑93 (MQ=255)
                                                                            cGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCgg             <  1:5396389/93‑1 (MQ=255)
                                                                            cGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCgg             >  2:1604994/1‑93 (MQ=255)
                                                                            cGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCgg             >  2:2193178/1‑93 (MQ=255)
                                                                             gCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGt            >  1:4870540/1‑93 (MQ=255)
                                                                             gCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGt            >  1:7935524/1‑93 (MQ=255)
                                                                             gCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGt            <  2:9769858/93‑1 (MQ=255)
                                                                              ccAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTa           >  2:7190353/1‑93 (MQ=255)
                                                                              ccAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTa           <  1:4096667/93‑1 (MQ=255)
                                                                              ccAAGCTGACGAAAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTa           <  1:3079357/93‑1 (MQ=255)
                                                                               cAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAg          >  1:7832769/1‑93 (MQ=255)
                                                                               cAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAg          <  2:5722284/93‑1 (MQ=255)
                                                                                aaGCTGACGAGAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGa         <  2:1713747/93‑1 (MQ=255)
                                                                                 aGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGaa        >  2:3561509/1‑93 (MQ=255)
                                                                                 aGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGaa        >  2:1111057/1‑93 (MQ=255)
                                                                                   cTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAg      <  2:7704041/93‑1 (MQ=255)
                                                                                     gACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCt    <  1:7375418/93‑1 (MQ=255)
                                                                                          |                                                                                         
CGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGAAAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGC  >  NC_000913/2690934‑2691113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTACGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGAAAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCGGC  >  NC_000913/2690931‑2691116
                                                                                             |                                                                                            
TTACGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGAT                                                                                              <  SRR4302540.7291226/94‑1 (MQ=60)
TTACGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGAT                                                                                              >  SRR4302540.8779310/1‑94 (MQ=60)
TTACGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGAT                                                                                              >  SRR4302540.2247735/1‑94 (MQ=60)
 TACGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATA                                                                                             >  SRR4302540.6331616/1‑94 (MQ=60)
   CGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAA                                                                                           >  SRR4302540.3596690/1‑94 (MQ=60)
   CGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAA                                                                                           >  SRR4302540.9835405/1‑94 (MQ=60)
   CGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAA                                                                                           <  SRR4302540.4200598/94‑1 (MQ=60)
   CGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAA                                                                                           <  SRR4302540.2079507/94‑1 (MQ=60)
    GCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAAC                                                                                          >  SRR4302540.9160239/1‑94 (MQ=60)
    GCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAAC                                                                                          >  SRR4302540.2136241/1‑94 (MQ=60)
    GCTGGGCAATCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTTCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAAC                                                                                          <  SRR4302540.3172028/94‑1 (MQ=60)
     CTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACC                                                                                         >  SRR4302540.5559216/1‑94 (MQ=60)
     CTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACC                                                                                         >  SRR4302540.1254734/1‑94 (MQ=60)
      TGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCT                                                                                        <  SRR4302540.6931130/94‑1 (MQ=60)
      TGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCT                                                                                        >  SRR4302540.8581003/1‑94 (MQ=60)
       atgaAACCAGCGCCTCAATCGCGTCCGAGAAGCCTTACAGTTGCGCGAGATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTG                                                                                       <  SRR4302540.6874711/90‑1 (MQ=60)
       GTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTG                                                                                       <  SRR4302540.6799773/94‑1 (MQ=60)
       GTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTG                                                                                       <  SRR4302540.3453377/94‑1 (MQ=60)
        TGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGC                                                                                      <  SRR4302540.5568915/94‑1 (MQ=60)
           AACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCC                                                                                   <  SRR4302540.9689345/94‑1 (MQ=60)
            ACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCA                                                                                  >  SRR4302540.2190251/1‑94 (MQ=60)
            ACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCA                                                                                  >  SRR4302540.6812558/1‑94 (MQ=60)
            ACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCA                                                                                  >  SRR4302540.5980127/1‑94 (MQ=60)
             CCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAG                                                                                 <  SRR4302540.3232807/94‑1 (MQ=60)
             CCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAG                                                                                 <  SRR4302540.5002737/94‑1 (MQ=60)
              CAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTTGCCAAGCTGACGATAAACCTGCGCCATC                                                                                >  SRR4302540.7295972/1‑94 (MQ=60)
              CAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGC                                                                                >  SRR4302540.7184877/1‑94 (MQ=60)
              CAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGC                                                                                <  SRR4302540.9463367/94‑1 (MQ=60)
                GCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGC                                                                              <  SRR4302540.3761086/94‑1 (MQ=60)
                 CGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCC                                                                             >  SRR4302540.4416261/1‑94 (MQ=60)
                  GCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCG                                                                            <  SRR4302540.6172620/94‑1 (MQ=60)
                   CCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGT                                                                           >  SRR4302540.1801736/1‑94 (MQ=60)
                    CTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTT                                                                          >  SRR4302540.8725318/1‑94 (MQ=60)
                      GAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGC                                                                        >  SRR4302540.4795908/1‑94 (MQ=60)
                        ATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGC                                                                      <  SRR4302540.3710952/94‑1 (MQ=60)
                             GTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGC                                                                 <  SRR4302540.3922629/94‑1 (MQ=60)
                             GTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGC                                                                 >  SRR4302540.4299883/1‑94 (MQ=60)
                              TCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCG                                                                <  SRR4302540.10017898/94‑1 (MQ=60)
                               CATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGC                                                               >  SRR4302540.1082269/1‑94 (MQ=60)
                               CATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGC                                                               >  SRR4302540.5590569/1‑94 (MQ=60)
                               CATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGC                                                               >  SRR4302540.4795208/1‑94 (MQ=60)
                               CATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGC                                                               >  SRR4302540.8504605/1‑94 (MQ=60)
                                ATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCG                                                              >  SRR4302540.543202/1‑94 (MQ=60)
                                ATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCG                                                              >  SRR4302540.8463278/1‑94 (MQ=60)
                                ATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCG                                                              >  SRR4302540.7366413/1‑94 (MQ=60)
                                ATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCG                                                              >  SRR4302540.2602048/1‑94 (MQ=60)
                                 TAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGT                                                             >  SRR4302540.6829093/1‑94 (MQ=60)
                                  AGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTT                                                            <  SRR4302540.7112497/94‑1 (MQ=60)
                                  AGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTT                                                            <  SRR4302540.235183/94‑1 (MQ=60)
                                   GAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTA                                                           >  SRR4302540.2460831/1‑94 (MQ=60)
                                    AAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTAT                                                          <  SRR4302540.5636105/94‑1 (MQ=60)
                                    AAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTAT                                                          <  SRR4302540.2934083/94‑1 (MQ=60)
                                    AAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTAT                                                          >  SRR4302540.2368696/1‑94 (MQ=60)
                                    AAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTAT                                                          >  SRR4302540.697409/1‑94 (MQ=60)
                                    AAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTAT                                                          >  SRR4302540.6597353/1‑94 (MQ=60)
                                     AGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATT                                                         <  SRR4302540.3243555/94‑1 (MQ=60)
                                     AGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATT                                                         >  SRR4302540.8301479/1‑94 (MQ=60)
                                     AGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATT                                                         <  SRR4302540.5720155/94‑1 (MQ=60)
                                     AGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATT                                                         <  SRR4302540.395082/94‑1 (MQ=60)
                                     AGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATT                                                         <  SRR4302540.2242818/94‑1 (MQ=60)
                                     AGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTACT                                                         >  SRR4302540.8338408/1‑94 (MQ=60)
                                      GCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTCTTG                                                        >  SRR4302540.9321784/1‑94 (MQ=60)
                                      GCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTG                                                        <  SRR4302540.4632346/94‑1 (MQ=60)
                                      GCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTG                                                        >  SRR4302540.2713675/1‑94 (MQ=60)
                                      GCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGACAGCGCCGTTGCGCCCCGCGCGTTATTC                                                        >  SRR4302540.7065115/1‑94 (MQ=60)
                                        CTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCC                                                      >  SRR4302540.7616331/1‑94 (MQ=60)
                                        CTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCC                                                      >  SRR4302540.7224829/1‑94 (MQ=60)
                                        CTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCC                                                      >  SRR4302540.5023284/1‑94 (MQ=60)
                                           TCAGTTGCGCGACATCGGGTACATCTGCCGGTTTGGCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCATTATTGCCTTT                                                   <  SRR4302540.2687856/94‑1 (MQ=60)
                                           TCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTT                                                   <  SRR4302540.3372417/94‑1 (MQ=60)
                                           TCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTT                                                   <  SRR4302540.6030731/94‑1 (MQ=60)
                                           TCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTT                                                   <  SRR4302540.8131765/94‑1 (MQ=60)
                                            CAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTG                                                  >  SRR4302540.2042035/1‑94 (MQ=60)
                                             AGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGC                                                 >  SRR4302540.7992116/1‑94 (MQ=60)
                                             AGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGC                                                 <  SRR4302540.210535/94‑1 (MQ=60)
                                              GTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCC                                                <  SRR4302540.7017886/94‑1 (MQ=60)
                                              GTTGCGCGACATCGCGCACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCC                                                <  SRR4302540.5518420/94‑1 (MQ=60)
                                                 GCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAA                                             <  SRR4302540.7745946/94‑1 (MQ=60)
                                                   GCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAAT                                           <  SRR4302540.7133691/94‑1 (MQ=60)
                                                    CGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATC                                          <  SRR4302540.618970/94‑1 (MQ=60)
                                                     GACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCA                                         >  SRR4302540.4994620/1‑94 (MQ=60)
                                                     GACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCA                                         >  SRR4302540.6076216/1‑94 (MQ=60)
                                                     CACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCA                                         <  SRR4302540.7241489/94‑1 (MQ=60)
                                                       CATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAAT                                       >  SRR4302540.2255627/1‑94 (MQ=60)
                                                        ATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATC                                      >  SRR4302540.9605769/1‑94 (MQ=60)
                                                        ATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATC                                      >  SRR4302540.9946200/1‑94 (MQ=60)
                                                        ATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATC                                      >  SRR4302540.3218908/1‑94 (MQ=60)
                                                         TCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCA                                     <  SRR4302540.2380184/94‑1 (MQ=60)
                                                         TCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCA                                     <  SRR4302540.2266829/94‑1 (MQ=60)
                                                         TCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCA                                     <  SRR4302540.920454/94‑1 (MQ=60)
                                                         TCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCA                                     <  SRR4302540.4854074/94‑1 (MQ=60)
                                                         TCGCGTACATCTGCCGGTTGGTCGCCAAGCGGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTGATTGCCTTGGCCCAAATCAATCA                                     <  SRR4302540.4854072/94‑1 (MQ=60)
                                                         TCGCGTACAGCTGCCGGGTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCA                                     <  SRR4302540.4854073/94‑1 (MQ=60)
                                                          CGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAG                                    <  SRR4302540.5488777/94‑1 (MQ=60)
                                                          CGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAG                                    <  SRR4302540.289934/94‑1 (MQ=60)
                                                           GCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGC                                   >  SRR4302540.9409300/1‑94 (MQ=60)
                                                           GCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGC                                   >  SRR4302540.4756146/1‑94 (MQ=60)
                                                           GCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGC                                   >  SRR4302540.9152311/1‑94 (MQ=60)
                                                            CGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCA                                  <  SRR4302540.592816/94‑1 (MQ=60)
                                                            CGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCA                                  <  SRR4302540.4363277/94‑1 (MQ=60)
                                                             GTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAG                                 >  SRR4302540.6318035/1‑94 (MQ=60)
                                                             GTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAG                                 <  SRR4302540.310984/94‑1 (MQ=60)
                                                                CATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAG                              <  SRR4302540.8365245/94‑1 (MQ=60)
                                                                CATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAG                              <  SRR4302540.2731301/94‑1 (MQ=60)
                                                                CATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAG                              <  SRR4302540.3990802/94‑1 (MQ=60)
                                                                 ATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGG                             >  SRR4302540.411997/1‑94 (MQ=60)
                                                                  TCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTG                            <  SRR4302540.2523415/94‑1 (MQ=60)
                                                                  TCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTG                            >  SRR4302540.713698/1‑94 (MQ=60)
                                                                   CTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGC                           <  SRR4302540.6908082/94‑1 (MQ=60)
                                                                   CTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGC                           >  SRR4302540.8059354/1‑94 (MQ=60)
                                                                     GCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGT                         <  SRR4302540.1629363/94‑1 (MQ=60)
                                                                      CCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTT                        <  SRR4302540.8511371/94‑1 (MQ=60)
                                                                       CGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTA                       <  SRR4302540.8118738/94‑1 (MQ=60)
                                                                        GGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTAT                      <  SRR4302540.8707867/94‑1 (MQ=60)
                                                                        GGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTAT                      <  SRR4302540.1765759/94‑1 (MQ=60)
                                                                        GGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTAT                      <  SRR4302540.1132908/94‑1 (MQ=60)
                                                                         GTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATC                     >  SRR4302540.8017845/1‑94 (MQ=60)
                                                                          TTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCT                    <  SRR4302540.2011892/94‑1 (MQ=60)
                                                                            TGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTC                  <  SRR4302540.1021595/94‑1 (MQ=60)
                                                                            TGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTC                  <  SRR4302540.7036888/94‑1 (MQ=60)
                                                                              TCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCTAAATCAATCAGCAGCAGTGCGTTATCTTCGG                >  SRR4302540.8870049/1‑94 (MQ=60)
                                                                               CGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGT               >  SRR4302540.8028015/1‑94 (MQ=60)
                                                                               CGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGT               >  SRR4302540.8028016/1‑94 (MQ=60)
                                                                               CGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGT               >  SRR4302540.4922788/1‑94 (MQ=60)
                                                                               CGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGT               <  SRR4302540.5455722/94‑1 (MQ=60)
                                                                                GCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTA              <  SRR4302540.3849685/94‑1 (MQ=60)
                                                                                 CCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAG             <  SRR4302540.4140860/94‑1 (MQ=60)
                                                                                 CCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAG             >  SRR4302540.7924290/1‑94 (MQ=60)
                                                                                 CCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAG             >  SRR4302540.2263020/1‑94 (MQ=60)
                                                                                 CCAAGCTGACGAAAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAG             <  SRR4302540.3113147/94‑1 (MQ=60)
                                                                                  CAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGA            >  SRR4302540.2596261/1‑94 (MQ=60)
                                                                                  CAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGA            >  SRR4302540.1485817/1‑94 (MQ=60)
                                                                                  CAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGA            >  SRR4302540.5431651/1‑94 (MQ=60)
                                                                                  CAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGA            <  SRR4302540.5696230/94‑1 (MQ=60)
                                                                                   AAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAA           <  SRR4302540.7156330/94‑1 (MQ=60)
                                                                                    AGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAA          <  SRR4302540.6235520/94‑1 (MQ=60)
                                                                                    AGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAA          <  SRR4302540.9717221/94‑1 (MQ=60)
                                                                                     GCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAG         >  SRR4302540.69223/1‑94 (MQ=60)
                                                                                      CTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGC        >  SRR4302540.2461099/1‑94 (MQ=60)
                                                                                      CTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGC        >  SRR4302540.7394209/1‑94 (MQ=60)
                                                                                        GACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTG      <  SRR4302540.7457619/94‑1 (MQ=60)
                                                                                          CGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCG    >  SRR4302540.299898/1‑94 (MQ=60)
                                                                                           GATAAACCTGCGCCAGCGCCTTTGCGCCCAGCGCTTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATC‑TCGGTAGAAAGCTGCGGC  <  SRR4302540.4326756/94‑1 (MQ=60)
                                                                                           GATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCGG   <  SRR4302540.502534/94‑1 (MQ=60)
                                                                                            ATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCGGC  >  SRR4302540.8615519/1‑94 (MQ=60)
                                                                                            ATAAACCGGCGCCAGCGCCGTTGCTCCCAGCGCGTTATTGCCTTTGCGCAAATCAACCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCGGC  <  SRR4302540.7574041/94‑1 (MQ=60)
                                                                                             |                                                                                            
TTACGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGAAAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCGGC  >  NC_000913/2690931‑2691116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑