Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,766,475 A→G T249A (ACC→GCC)  b2632 → putative GTP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,766,4750AG99.6% 773.5 / NA 240T249A (ACC→GCC) b2632putative GTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (136/103);  minor base T (0/1);  total (136/104)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 247 total aligned reads displayed.

GCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCA  >  NC_000913/2766386‑2766561
                                                                                         |                                                                                      
gcagcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCa                                                                                     <  1:6698106/93‑1 (MQ=255)
gcagcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCa                                                                                     >  1:8774340/1‑93 (MQ=255)
 cagcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCAt                                                                                    <  1:2769033/93‑1 (MQ=255)
   gcCCGGTAGTAGCGCTACTGCAACATCCCTTTTGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGCGAAACCGTAGGTGCCATAc                                                                                  >  2:6820256/1‑93 (MQ=255)
   gcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTCGGTGCCATAc                                                                                  >  1:8412691/1‑93 (MQ=255)
    cccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACt                                                                                 >  2:7418640/1‑93 (MQ=255)
    cccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACt                                                                                 <  1:8593818/93‑1 (MQ=255)
    cccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACt                                                                                 >  1:2291753/1‑93 (MQ=255)
     ccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTc                                                                                >  2:7108825/1‑93 (MQ=255)
      cGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCg                                                                               >  2:6759132/1‑93 (MQ=255)
      cGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCg                                                                               >  1:4489969/1‑93 (MQ=255)
       ggTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGa                                                                              >  2:5739750/1‑93 (MQ=255)
         tagtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATa                                                                            <  2:1079661/93‑1 (MQ=255)
         tagtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATa                                                                            <  2:5304924/93‑1 (MQ=255)
         tagtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATa                                                                            <  2:7591913/93‑1 (MQ=255)
         tagtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATa                                                                            <  1:3784029/93‑1 (MQ=255)
            tagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACgg                                                                         >  1:7262414/1‑93 (MQ=255)
            tagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACgg                                                                         >  1:7205147/1‑93 (MQ=255)
            tagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACgg                                                                         >  1:9912336/1‑93 (MQ=255)
            tagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACgg                                                                         <  2:3238862/93‑1 (MQ=255)
             agCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGt                                                                        <  1:6527594/93‑1 (MQ=255)
               cgcTACTGCAACTTCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTaa                                                                      <  2:8117755/93‑1 (MQ=255)
               cgcTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTaa                                                                      <  1:548467/93‑1 (MQ=255)
                 cTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGATGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGc                                                                    <  2:6758772/93‑1 (MQ=255)
                 cTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGc                                                                    >  2:300470/1‑93 (MQ=255)
                 cTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGc                                                                    <  2:9419296/93‑1 (MQ=255)
                   aCTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCAc                                                                  >  1:1363994/1‑93 (MQ=255)
                     tGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCt                                                                >  1:5790978/1‑93 (MQ=255)
                     tGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCt                                                                <  1:1245357/93‑1 (MQ=255)
                     tGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGAAATCACCt                                                                >  1:6244604/1‑93 (MQ=255)
                      gCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCCCCtt                                                               >  2:6286179/1‑93 (MQ=255)
                      gCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCtt                                                               >  2:3344366/1‑93 (MQ=255)
                      gCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCtt                                                               <  1:2157923/93‑1 (MQ=255)
                      gCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCtt                                                               <  2:645355/93‑1 (MQ=255)
                        aaCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTc                                                             >  1:2422338/1‑93 (MQ=255)
                         aCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTcc                                                            >  2:5819707/1‑93 (MQ=255)
                         aCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTcc                                                            >  2:9201403/1‑93 (MQ=255)
                         aCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTcc                                                            <  2:4408356/93‑1 (MQ=255)
                         aCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTcc                                                            >  1:764683/1‑93 (MQ=255)
                         aCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTcc                                                            >  1:7383384/1‑93 (MQ=255)
                           aTCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTcccc                                                          <  2:5253624/93‑1 (MQ=255)
                           aTCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTcccc                                                          >  1:7369363/1‑93 (MQ=255)
                                 ttCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTc                                                    <  2:306947/93‑1 (MQ=255)
                                       caacggTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGccc                                              >  2:2303843/5‑93 (MQ=255)
                                         acggTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCg                                            >  2:4528822/3‑93 (MQ=255)
                                          cggTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCgg                                           >  1:9413036/2‑93 (MQ=255)
                                          cggTAGCCCGGGAGCAGGCACGTGACGATTTCAGTGAAACCGTAGGTGCCATCCTCGATGCGGTAAGCGCCTTTCCCCTTATTCCCGCCCCgg                                           <  2:629112/92‑1 (MQ=255)
                                           ggTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGt                                          >  1:8318440/1‑93 (MQ=255)
                                           ggTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGt                                          <  2:8892197/93‑1 (MQ=255)
                                            gTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTg                                         >  1:5939682/1‑93 (MQ=255)
                                            gTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTg                                         <  1:7493714/93‑1 (MQ=255)
                                             tAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGc                                        <  2:3774287/93‑1 (MQ=255)
                                               gCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCgg                                      >  1:9485454/1‑93 (MQ=255)
                                                cccGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGa                                     >  2:8169702/1‑93 (MQ=255)
                                                  cGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGAcc                                   >  1:9924315/1‑93 (MQ=255)
                                                  cGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGAcc                                   >  2:345383/1‑93 (MQ=255)
                                                   gggAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCa                                  >  1:7249318/1‑93 (MQ=255)
                                                    ggAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCat                                 >  2:1802055/1‑93 (MQ=255)
                                                    ggAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCat                                 >  1:1767087/1‑93 (MQ=255)
                                                     gaccAGGCGTGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCGCCTTTCCCCTTATTCCCGCCCCGGTGCGGACCatt                                <  2:97151/90‑1 (MQ=25)
                                                     gAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCatt                                >  2:4844237/1‑93 (MQ=255)
                                                      aGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCatta                               <  1:5310464/93‑1 (MQ=255)
                                                       gCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCattat                              >  2:6204052/1‑93 (MQ=255)
                                                        cAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCattctt                             >  1:332090/1‑93 (MQ=255)
                                                        cAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCattatt                             >  1:7100944/1‑93 (MQ=255)
                                                         aGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTc                            >  1:2324715/1‑93 (MQ=255)
                                                         aGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTc                            <  2:5184195/93‑1 (MQ=255)
                                                          gtcACGTGACGATTTCGGTGAAACCGTAGGTGCCATCCTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCa                           <  1:4445066/91‑1 (MQ=37)
                                                          ggCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTGTTCa                           >  2:9186587/1‑93 (MQ=255)
                                                          ggCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCa                           >  2:3569983/1‑93 (MQ=255)
                                                          ggCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCa                           >  2:6030621/1‑93 (MQ=255)
                                                          ggCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCa                           >  1:7706145/1‑93 (MQ=255)
                                                             aCGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCAGGTGCGGACCATTATTCAGGc                        >  2:658366/1‑93 (MQ=255)
                                                             aCGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCCTATTCCCGCCCCGGTGCGGACCATTATTCAGGc                        >  1:6761862/1‑93 (MQ=255)
                                                              cGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGcc                       >  1:5646351/1‑93 (MQ=255)
                                                               gTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCg                      <  2:4994498/93‑1 (MQ=255)
                                                                tGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGt                     >  1:4959469/1‑93 (MQ=255)
                                                                tGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGt                     >  2:3986011/1‑93 (MQ=255)
                                                                 gACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTg                    >  1:9984710/1‑93 (MQ=255)
                                                                 gACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTg                    <  1:546892/93‑1 (MQ=255)
                                                                    gATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGt                 >  1:4623734/1‑93 (MQ=255)
                                                                    gATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGt                 <  2:7867955/93‑1 (MQ=255)
                                                                    gATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGt                 <  2:2494337/93‑1 (MQ=255)
                                                                    gATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGt                 >  2:6124342/1‑93 (MQ=255)
                                                                     aTTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGtt                >  2:6368667/1‑93 (MQ=255)
                                                                     aTTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGtt                >  1:3747416/1‑93 (MQ=255)
                                                                      tttCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTc               >  1:7660042/1‑93 (MQ=255)
                                                                       ttCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTcc              <  1:7349455/93‑1 (MQ=255)
                                                                        tCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCt             >  2:555542/1‑93 (MQ=255)
                                                                          ggTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCg           >  1:1879113/1‑93 (MQ=255)
                                                                           gTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCgg          >  1:1004108/1‑93 (MQ=255)
                                                                           gTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCgg          <  1:6084256/93‑1 (MQ=255)
                                                                            tGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCggt         <  2:7100163/93‑1 (MQ=255)
                                                                            tGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCggt         <  1:3102874/93‑1 (MQ=255)
                                                                            tGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCggt         <  1:9621818/93‑1 (MQ=255)
                                                                             gAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTACCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCggtg        >  2:8592117/1‑93 (MQ=255)
                                                                               aaCCGTAGGTGCCATACTCGATACGGTAAGTACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCggtggt      >  2:1627128/1‑93 (MQ=255)
                                                                                aCCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGgtggtg     <  1:28325/93‑1 (MQ=255)
                                                                                   gTAGTTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCa  <  2:7309701/93‑1 (MQ=255)
                                                                                   gTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCa  >  2:3483691/1‑93 (MQ=255)
                                                                                         |                                                                                      
GCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCA  >  NC_000913/2766386‑2766561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCC  >  NC_000913/2766385‑2766568
                                                                                          |                                                                                             
AGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCA                                                                                            >  SRR4302540.8875987/1‑94 (MQ=60)
 GCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCAT                                                                                           <  SRR4302540.6772042/94‑1 (MQ=60)
 GCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCAT                                                                                           <  SRR4302540.6772043/94‑1 (MQ=60)
  CAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATA                                                                                          <  SRR4302540.2798958/94‑1 (MQ=60)
   AGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTCGGTGCCATAC                                                                                         >  SRR4302540.8510338/1‑94 (MQ=60)
   AGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAC                                                                                         >  SRR4302540.310694/1‑94 (MQ=60)
    GCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACT                                                                                        >  SRR4302540.5020801/1‑94 (MQ=60)
    GCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACT                                                                                        >  SRR4302540.2316418/1‑94 (MQ=60)
     CCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTC                                                                                       <  SRR4302540.8693716/94‑1 (MQ=60)
     CCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTC                                                                                       >  SRR4302540.7311882/1‑94 (MQ=60)
     CCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTC                                                                                       >  SRR4302540.7311885/1‑94 (MQ=60)
      CCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCG                                                                                      >  SRR4302540.4538660/1‑94 (MQ=60)
       CGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGA                                                                                     <  SRR4302540.9570083/94‑1 (MQ=60)
       CGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGA                                                                                     <  SRR4302540.8632487/94‑1 (MQ=60)
       CGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGA                                                                                     >  SRR4302540.7737605/1‑94 (MQ=60)
       CGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGA                                                                                     >  SRR4302540.7737604/1‑94 (MQ=60)
        GGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGAT                                                                                    >  SRR4302540.9401897/1‑94 (MQ=60)
        GGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGAT                                                                                    <  SRR4302540.10098375/94‑1 (MQ=60)
        GGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGAT                                                                                    <  SRR4302540.9672477/94‑1 (MQ=60)
         GTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATA                                                                                   <  SRR4302540.3221419/94‑1 (MQ=60)
         GTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATA                                                                                   <  SRR4302540.4525889/94‑1 (MQ=60)
          TGGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAC                                                                                  <  SRR4302540.3825297/94‑1 (MQ=60)
          TAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAC                                                                                  <  SRR4302540.7110457/94‑1 (MQ=60)
          TAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAC                                                                                  <  SRR4302540.3825298/94‑1 (MQ=60)
          TAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAC                                                                                  >  SRR4302540.8660806/1‑94 (MQ=60)
           AGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACG                                                                                 <  SRR4302540.5772093/94‑1 (MQ=60)
           AGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACG                                                                                 <  SRR4302540.3668387/94‑1 (MQ=60)
            GTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGG                                                                                >  SRR4302540.10028111/1‑94 (MQ=60)
            GTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGG                                                                                >  SRR4302540.7343444/1‑94 (MQ=60)
            GTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGG                                                                                >  SRR4302540.7285470/1‑94 (MQ=60)
            GTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGG                                                                                >  SRR4302540.5669804/1‑94 (MQ=60)
              AGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTA                                                                              <  SRR4302540.6600084/94‑1 (MQ=60)
                CGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAG                                                                            <  SRR4302540.554443/94‑1 (MQ=60)
                CGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAG                                                                            <  SRR4302540.6746055/94‑1 (MQ=60)
                 GCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGC                                                                           >  SRR4302540.4224297/1‑94 (MQ=60)
                   TACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCAC                                                                         >  SRR4302540.1378406/1‑94 (MQ=60)
                   TACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCAC                                                                         >  SRR4302540.1378407/1‑94 (MQ=60)
                   TACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCAC                                                                         <  SRR4302540.219928/94‑1 (MQ=60)
                   TACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTAGATACGGTAAGCAC                                                                         >  SRR4302540.1790482/1‑94 (MQ=60)
                    ACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACC                                                                        <  SRR4302540.991303/94‑1 (MQ=60)
                    ACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACC                                                                        <  SRR4302540.7439725/94‑1 (MQ=60)
                     CTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCT                                                                       >  SRR4302540.2813283/1‑94 (MQ=60)
                     CTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCT                                                                       >  SRR4302540.5854961/1‑94 (MQ=60)
                     CTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCT                                                                       >  SRR4302540.9641298/1‑94 (MQ=60)
                     CTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCT                                                                       >  SRR4302540.7639101/1‑94 (MQ=60)
                     CTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGAAATCACCT                                                                       >  SRR4302540.6314095/1‑94 (MQ=60)
                      TGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTT                                                                      <  SRR4302540.1258060/94‑1 (MQ=60)
                       GCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTT                                                                     <  SRR4302540.2181159/94‑1 (MQ=60)
                       GCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTT                                                                     <  SRR4302540.5429635/94‑1 (MQ=60)
                        CAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTC                                                                    >  SRR4302540.2448437/1‑94 (MQ=60)
                         AACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCC                                                                   >  SRR4302540.7570791/1‑94 (MQ=60)
                         AACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCC                                                                   >  SRR4302540.6397318/1‑94 (MQ=60)
                         AACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCC                                                                   >  SRR4302540.7465652/1‑94 (MQ=60)
                         AACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCC                                                                   >  SRR4302540.772825/1‑94 (MQ=60)
                          ACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCC                                                                  >  SRR4302540.830015/1‑94 (MQ=60)
                           CATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCC                                                                 >  SRR4302540.7451511/1‑94 (MQ=60)
                            ATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCT                                                                >  SRR4302540.5535142/1‑94 (MQ=60)
                            ATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCT                                                                >  SRR4302540.4631672/1‑94 (MQ=60)
                                CTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATT                                                            >  SRR4302540.8480142/1‑94 (MQ=60)
                                CTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATT                                                            <  SRR4302540.3385616/94‑1 (MQ=60)
                                  ttcgaacaacGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCC                                                          <  SRR4302540.2429511/84‑1 (MQ=60)
                                   tcgaacaacGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCGCCTTTCCCCTTATTCCC                                                         <  SRR4302540.5938219/85‑1 (MQ=60)
                                   tcgaacaacGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCC                                                         <  SRR4302540.5463775/85‑1 (MQ=60)
                                   tcgaacaacGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCC                                                         <  SRR4302540.9521697/85‑1 (MQ=60)
                                     gaacaacGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTCTCCCCTTATTCCCGC                                                       >  SRR4302540.9539414/8‑94 (MQ=60)
                                      aacaacGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCC                                                      >  SRR4302540.8554266/7‑94 (MQ=60)
                                      aacaacGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCC                                                      <  SRR4302540.8187611/88‑1 (MQ=60)
                                       acaacGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCC                                                     <  SRR4302540.8715077/89‑1 (MQ=60)
                                        caacGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCC                                                    >  SRR4302540.1465938/5‑94 (MQ=60)
                                          acGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGG                                                  >  SRR4302540.9524953/3‑94 (MQ=60)
                                           CGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGT                                                 >  SRR4302540.8415004/1‑94 (MQ=60)
                                            GGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTG                                                >  SRR4302540.2772167/1‑94 (MQ=60)
                                            GGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTG                                                >  SRR4302540.6005343/1‑94 (MQ=60)
                                            GGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTG                                                >  SRR4302540.6005344/1‑94 (MQ=60)
                                             GTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGC                                               <  SRR4302540.7577407/94‑1 (MQ=60)
                                             GTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGC                                               <  SRR4302540.204205/94‑1 (MQ=60)
                                               AGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGG                                             >  SRR4302540.9597917/1‑94 (MQ=60)
                                                  CCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACC                                          >  SRR4302540.10040187/1‑94 (MQ=60)
                                                   CGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCA                                         >  SRR4302540.7342099/1‑94 (MQ=60)
                                                   CGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCA                                         >  SRR4302540.7330211/1‑94 (MQ=60)
                                                    GGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCAT                                        >  SRR4302540.1786259/1‑94 (MQ=60)
                                                     GGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATT                                       <  SRR4302540.8186035/94‑1 (MQ=60)
                                                      GAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTA                                      <  SRR4302540.6055221/94‑1 (MQ=60)
                                                       AGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTAT                                     <  SRR4302540.5368589/94‑1 (MQ=60)
                                                       AGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTAT                                     <  SRR4302540.6956024/94‑1 (MQ=60)
                                                        GCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTCTT                                    >  SRR4302540.335436/1‑94 (MQ=60)
                                                        GCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATT                                    <  SRR4302540.7526201/94‑1 (MQ=60)
                                                        GCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATT                                    >  SRR4302540.7179955/1‑94 (MQ=60)
                                                         CAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTC                                   >  SRR4302540.2349485/1‑94 (MQ=60)
                                                         CAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTC                                   >  SRR4302540.2948770/1‑94 (MQ=60)
                                                         CAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTC                                   >  SRR4302540.2349752/1‑94 (MQ=60)
                                                         CAGGCACGTGACGATTTCGGTGAAACCGTAGCTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTC                                   >  SRR4302540.6291586/1‑94 (MQ=60)
                                                          AGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCA                                  >  SRR4302540.7356345/1‑94 (MQ=60)
                                                          AGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCA                                  >  SRR4302540.74347/1‑94 (MQ=60)
                                                          AGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCA                                  >  SRR4302540.7794452/1‑94 (MQ=60)
                                                          AGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCA                                  >  SRR4302540.5878322/1‑94 (MQ=60)
                                                           GTCACGTGACGATTTCGGTGAAACCGTAGGTGCCATCCTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAG                                 <  SRR4302540.4493240/94‑1 (MQ=60)
                                                           GGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAG                                 <  SRR4302540.5482528/94‑1 (MQ=60)
                                                             CACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCCTATTCCCGCCCCGGTGCGGACCATTATTCAGGC                               >  SRR4302540.6836592/1‑94 (MQ=60)
                                                              ACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCC                              >  SRR4302540.5708496/1‑94 (MQ=60)
                                                               CGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCG                             >  SRR4302540.10034499/1‑94 (MQ=60)
                                                               CGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCAG                             >  SRR4302540.10034353/1‑94 (MQ=60)
                                                                GTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGT                            >  SRR4302540.5013088/1‑94 (MQ=60)
                                                                GTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGT                            <  SRR4302540.231767/94‑1 (MQ=60)
                                                                 TGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTG                           >  SRR4302540.10101118/1‑94 (MQ=60)
                                                                  GACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGC                          <  SRR4302540.552846/94‑1 (MQ=60)
                                                                  GACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGC                          <  SRR4302540.2167948/94‑1 (MQ=60)
                                                                  GACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGC                          >  SRR4302540.6239104/1‑94 (MQ=60)
                                                                  GACGATTTCGGTGAAACCGTAGGGGCCATACTCGATACGGTAGGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGC                          >  SRR4302540.4749758/1‑94 (MQ=60)
                                                                    CGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGT                        >  SRR4302540.5594936/1‑94 (MQ=60)
                                                                    CGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGT                        >  SRR4302540.4673284/1‑94 (MQ=60)
                                                                    CGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGT                        >  SRR4302540.1273064/1‑94 (MQ=60)
                                                                    CGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGT                        <  SRR4302540.7771272/94‑1 (MQ=60)
                                                                     GATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTT                       >  SRR4302540.3788309/1‑94 (MQ=60)
                                                                      ATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTC                      >  SRR4302540.7746299/1‑94 (MQ=60)
                                                                        TTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCT                    <  SRR4302540.8854057/94‑1 (MQ=60)
                                                                        TTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCT                    <  SRR4302540.84307/94‑1 (MQ=60)
                                                                        TTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCT                    <  SRR4302540.7431447/94‑1 (MQ=60)
                                                                        TTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCT                    >  SRR4302540.4121598/1‑94 (MQ=60)
                                                                          CGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCG                  >  SRR4302540.1899693/1‑94 (MQ=60)
                                                                           GGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGG                 >  SRR4302540.2773794/1‑94 (MQ=60)
                                                                           GGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGG                 <  SRR4302540.6809531/94‑1 (MQ=60)
                                                                           GGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGG                 >  SRR4302540.1014299/1‑94 (MQ=60)
                                                                            GTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGT                <  SRR4302540.6151968/94‑1 (MQ=60)
                                                                            GTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGT                >  SRR4302540.7451646/1‑94 (MQ=60)
                                                                             TGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTG               <  SRR4302540.9735231/94‑1 (MQ=60)
                                                                             TGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTG               <  SRR4302540.3136851/94‑1 (MQ=60)
                                                                             TGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTG               >  SRR4302540.4939833/1‑94 (MQ=60)
                                                                              GAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGG              <  SRR4302540.1219289/94‑1 (MQ=60)
                                                                              GAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGACCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGG              >  SRR4302540.943499/1‑94 (MQ=60)
                                                                                 ACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGT           <  SRR4302540.28627/94‑1 (MQ=60)
                                                                                    GTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAG        >  SRR4302540.1273408/1‑94 (MQ=60)
                                                                                    GTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAG        <  SRR4302540.7193464/94‑1 (MQ=60)
                                                                                    GTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAG        >  SRR4302540.175928/1‑94 (MQ=60)
                                                                                    GTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAG        >  SRR4302540.7406147/1‑94 (MQ=60)
                                                                                     TAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGT       >  SRR4302540.7571441/1‑94 (MQ=60)
                                                                                     TAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGT       <  SRR4302540.2061527/94‑1 (MQ=60)
                                                                                     TAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGT       <  SRR4302540.5985233/94‑1 (MQ=60)
                                                                                     TAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATCCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGT       <  SRR4302540.3736833/94‑1 (MQ=60)
                                                                                         TGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCC   >  SRR4302540.1465159/1‑94 (MQ=60)
                                                                                         TGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCC   >  SRR4302540.2752616/1‑94 (MQ=60)
                                                                                          GCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCC  <  SRR4302540.9120339/94‑1 (MQ=60)
                                                                                          |                                                                                             
AGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCC  >  NC_000913/2766385‑2766568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑