Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,790,474	T→C	L394P (CTG→CCG)	gabD →	succinate‑semialdehyde dehydrogenase, NADP‑dependent activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,790,474	0	T	C	99.1%	721.3 / ‑2.9	230	L394P (CTG→CCG)	gabD	succinate‑semialdehyde dehydrogenase, NADP‑dependent activity
Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (136/91); total (139/91)
Fisher's exact test for biased strand distribution p-value = 5.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.36e-01

Only 100 of 263 total aligned reads displayed.

GCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCTGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTGATTTAAGC  >  NC_000913/2790384‑2790565

gcggcAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGt                                                                                           >  1:9328476/1‑93 (MQ=255)

gcggcAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGt                                                                                           >  1:8232174/1‑93 (MQ=255)

gcggcAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGt                                                                                           >  1:226589/1‑93 (MQ=255)

  ggcAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGGCCCCTCGCCCCCCCGTTc                                                                                         >  2:7582179/1‑93 (MQ=255)

     aaCTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCACAGCGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGc                                                                                      >  1:3446878/1‑93 (MQ=255)

      aCTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCt                                                                                     >  1:7261297/1‑93 (MQ=255)

       cttcttCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCtt                                                                                    <  2:6438828/93‑1 (MQ=255)

       cttcttCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCtt                                                                                    >  2:7764021/1‑93 (MQ=255)

        ttcttcCAGCCGACCATTCTGGTGGACGTTCCGGGCAACGCCAAAGTGTCGACAGAAGAGACGTT‑CGGACCCCTCGCCCCGCCGTTCCGCttt                                                                                   >  1:8467872/1‑93 (MQ=255)

        ttcttcCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCttt                                                                                   <  2:3646678/93‑1 (MQ=255)

         tcttcCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTa                                                                                  >  2:7468159/1‑93 (MQ=255)

          cctcCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTaa                                                                                 <  1:5871526/91‑1 (MQ=255)

            tcCAGCCGA‑CATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGa                                                                              >  1:5715775/1‑93 (MQ=255)

             ccAGCCGATCATTGTGGTGGACGTCCCGTCCAACGCCAAAGTGTCGAAAGAAGAGACGTG‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGa                                                                              <  2:3000835/93‑1 (MQ=255)

              cgtccGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGAt                                                                             <  2:4527971/90‑1 (MQ=255)

              cAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGAt                                                                             >  2:7819236/1‑93 (MQ=255)

              cAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGAt                                                                             <  1:4085424/93‑1 (MQ=255)

               aGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATg                                                                            <  1:3119012/93‑1 (MQ=255)

                gCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGa                                                                           >  1:891541/1‑93 (MQ=255)

                  cGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAg                                                                         >  1:1736190/1‑93 (MQ=255)

                   gACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGc                                                                        >  1:2484530/1‑93 (MQ=255)

                   gACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGc                                                                        <  1:3644699/93‑1 (MQ=255)

                   caCCATTCTGGTGGACGTTCCGGCCAACGCCAAGGGGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGc                                                                        <  1:2108422/92‑1 (MQ=255)

                      cATTCTGATGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGa                                                                     <  2:4227307/93‑1 (MQ=255)

                       aTTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGAt                                                                    <  1:7666755/93‑1 (MQ=255)

                        ttCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGAtg                                                                   >  1:9989070/1‑93 (MQ=255)

                        ttCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGAtg                                                                   >  1:6108807/1‑93 (MQ=255)

                        ttCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGAtg                                                                   >  2:8417363/1‑93 (MQ=255)

                           tggtggACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACTTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGa                                                                <  1:723647/93‑1 (MQ=255)

                           tggtggACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGa                                                                <  2:481316/93‑1 (MQ=255)

                             gtggACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGAtt                                                              <  1:5025027/93‑1 (MQ=255)

                             gtggACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGAtt                                                              >  1:4739278/1‑93 (MQ=255)

                              tggACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTg                                                             >  2:3197358/1‑93 (MQ=255)

                              tggACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTg                                                             <  1:1582654/93‑1 (MQ=255)

                               ggACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTgc                                                            >  2:1432834/1‑93 (MQ=255)

                               ggACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTgc                                                            >  1:6993686/1‑93 (MQ=255)

                               ggACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTgc                                                            >  2:2618925/1‑93 (MQ=255)

                               ggACGTGCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTgc                                                            <  2:4294943/93‑1 (MQ=255)

                                   gTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCaa                                                        >  1:8047517/1‑93 (MQ=255)

                                    ttCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAg                                                       <  2:1695962/93‑1 (MQ=255)

                                    ttCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAg                                                       >  1:861270/1‑93 (MQ=255)

                                    ttCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAg                                                       >  1:6834019/1‑93 (MQ=255)

                                     tCCGGCCAACGCCAAAGTGTCGAAAGAAGAGCCGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGc                                                      <  2:4029415/93‑1 (MQ=255)

                                      ccGGCCAACGCCAAAGTGTCAAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGcc                                                     <  2:8312921/93‑1 (MQ=255)

                                       cgcccAACGCCAAAGTGTCAAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCa                                                    <  2:8231384/90‑1 (MQ=255)

                                       cGGCCAACGCCAAAGTGTTGAAAGACGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAAccc                                                    >  2:764382/1‑92 (MQ=255)

                                        ggCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCACGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCaa                                                   <  1:3213595/93‑1 (MQ=255)

                                         gCCAACGCAAAAGTGTCAAAAAAAGAGCCGTT‑CGGCCCCCTCCCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAAt                                                  <  2:2002029/93‑1 (MQ=255)

                                          ccAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATg                                                 >  2:9916766/1‑93 (MQ=255)

                                          ccAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATg                                                 <  2:8563189/93‑1 (MQ=255)

                                           cAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGa                                                >  2:8895263/1‑93 (MQ=255)

                                           cAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGa                                                >  1:2954641/1‑93 (MQ=255)

                                           cAACGCCAAAGTGTCAAAAGAAGAGACGTT‑CGCCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGa                                                <  2:675457/93‑1 (MQ=255)

                                            aaCGCCTAAGCGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCGAGCCAATGac                                               >  2:9282438/1‑93 (MQ=255)

                                             aCGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGaca                                              >  2:5470473/1‑93 (MQ=255)

                                             aCGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGaca                                              >  1:4390450/1‑93 (MQ=255)

                                              cGCCAAAGTGTCAAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGacac                                             <  1:1034387/93‑1 (MQ=255)

                                               cccAATGTGCAGAAAGAAG‑GCCGTTCCGCCCCCCCCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACAcc                                            <  2:229375/92‑1 (MQ=255)

                                                 cAAGGTGTCGAAAGAAGAGACTTT‑CGCCCCCCCCCCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGa                                          <  2:978620/93‑1 (MQ=255)

                                                 cAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGa                                          >  1:1384190/1‑93 (MQ=255)

                                                  aaaGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAg                                         >  2:3945183/1‑93 (MQ=255)

                                                  aaaGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAg                                         >  2:2106721/1‑93 (MQ=255)

                                                        tCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGc                                   >  2:4246126/1‑93 (MQ=255)

                                                        tCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGc                                   >  1:9293605/1‑93 (MQ=255)

                                                         cGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGcc                                  <  1:5145107/93‑1 (MQ=255)

                                                         cGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGcc                                  >  1:9783145/1‑93 (MQ=255)

                                                          gAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCt                                 >  1:2782359/1‑93 (MQ=255)

                                                          gAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCt                                 >  1:7950755/1‑93 (MQ=255)

                                                          gAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCt                                 >  2:1908361/1‑93 (MQ=255)

                                                          aaaaaaaGAGAGGTT‑CGCCCCCCGCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCt                                 <  2:80223/92‑1 (MQ=255)

                                                            aagaagAGACTTT‑CGCCCCCCTCCCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTg                               <  1:7626845/93‑1 (MQ=255)

                                                            aagaagAGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTg                               >  2:8105281/1‑93 (MQ=255)

                                                             agaagaGACGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTgc                              >  2:9520453/1‑93 (MQ=255)

                                                             agaagaGACGTG‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTgc                              <  1:5311342/93‑1 (MQ=255)

                                                                 gagaCGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTgccgcc                          >  1:9568222/1‑93 (MQ=255)

                                                                   gagttt‑CGCCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTa                        <  1:4772890/89‑1 (MQ=255)

                                                                   accGTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTa                        <  1:2841961/91‑1 (MQ=255)

                                                                      gTT‑CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTCGTCCTGCCGCCTAttt                     >  1:5613023/1‑93 (MQ=255)

                                                                        ttgggCCCCCCCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTc                    <  1:5393183/90‑1 (MQ=255)

                                                                           ggCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGAGCAAGCCAATGACACCGAGTTTGGCCTTGCCGACTATTTCTAc                 >  2:9693689/1‑93 (MQ=255)

                                                                            gCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACg                >  2:5630868/1‑93 (MQ=255)

                                                                               cccTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGccc             >  2:594388/1‑93 (MQ=255)

                                                                                ccTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGGCCg            >  2:5027483/1‑93 (MQ=255)

                                                                                 cTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGt           >  2:4614893/1‑93 (MQ=255)

                                                                                 cTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGt           >  1:600768/1‑93 (MQ=255)

                                                                                 cTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGt           >  2:2531092/1‑93 (MQ=255)

                                                                                  tCGCCCCGCTGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTg          >  2:862039/1‑93 (MQ=255)

                                                                                  tCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCggg          >  2:4855691/1‑91 (MQ=255)

                                                                                  tCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTg          >  2:7572724/1‑93 (MQ=255)

                                                                                  tCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTg          >  2:5265306/1‑93 (MQ=255)

                                                                                  cccccccGCCGTGCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTg          <  1:6280010/90‑1 (MQ=255)

                                                                                     ccccccCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTGCGCCCGTGAtt       <  1:671759/93‑1 (MQ=255)

                                                                                     ccccGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTGAtt       <  2:2856109/93‑1 (MQ=255)

                                                                                     ccccGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTGAtt       <  2:3445404/93‑1 (MQ=255)

                                                                                      cccGCCGTGCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTGAttt      <  2:9226923/93‑1 (MQ=255)

                                                                                       ccgccgTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTGATTTa     >  1:3699378/1‑93 (MQ=255)

                                                                                       ccgccgTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTGATTTa     <  2:5126699/93‑1 (MQ=255)

                                                                                        cGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTGATTTag    >  2:5874151/1‑92 (MQ=255)

                                                                                        cGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTGATTTaa    <  2:6543095/93‑1 (MQ=255)

                                                                                        cGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTGATTTaa    >  1:4505938/1‑93 (MQ=255)

GCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTT‑CGGCCCCCTCGCCCCGCTGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTGATTTAAGC  >  NC_000913/2790384‑2790565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTGATTTAAGCCG  >  NC_000913/2790381‑2790567

GCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCT                                                                                               <  SRR4302540.1928150/94‑1 (MQ=60)

GCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCC                                                                                               <  SRR4302540.5464527/94‑1 (MQ=60)

  GGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGT                                                                                             >  SRR4302540.8327858/1‑94 (MQ=60)

  GGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGT                                                                                             >  SRR4302540.9439716/1‑94 (MQ=60)

  GGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGT                                                                                             >  SRR4302540.9439715/1‑94 (MQ=60)

  GGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGT                                                                                             >  SRR4302540.228777/1‑94 (MQ=60)

     GGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCC                                                                                          >  SRR4302540.7817870/1‑94 (MQ=60)

      GCACCTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCG                                                                                         <  SRR4302540.646643/94‑1 (MQ=60)

      GCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCG                                                                                         <  SRR4302540.9242483/94‑1 (MQ=60)

       CAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCACAGCGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGC                                                                                        >  SRR4302540.3484371/1‑94 (MQ=60)

        AACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCT                                                                                       >  SRR4302540.7342317/1‑94 (MQ=60)

        AACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCT                                                                                       >  SRR4302540.1997168/1‑94 (MQ=60)

        AACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCT                                                                                       >  SRR4302540.506780/1‑94 (MQ=60)

          CTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGGCAACGCCAAAGTGTCGACAGAAGAGACGTTCGGACCCCTCGCCCCGCCGTTCCGCTTT                                                                                     >  SRR4302540.8566193/1‑94 (MQ=60)

          CTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTT                                                                                     >  SRR4302540.8566189/1‑94 (MQ=60)

             CCTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAA                                                                                  <  SRR4302540.5936428/94‑1 (MQ=60)

              TTCCAGCCGA‑CATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGA                                                                                >  SRR4302540.5778692/1‑94 (MQ=60)

               TCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGA                                                                                <  SRR4302540.4959928/94‑1 (MQ=60)

               TCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGACCCCATCGCCCCGCGGTTCCGCTTTcaaca                                                                                >  SRR4302540.8303532/1‑89 (MQ=60)

                 CAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATG                                                                              <  SRR4302540.5179114/94‑1 (MQ=60)

                 CAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATG                                                                              <  SRR4302540.4129484/94‑1 (MQ=60)

                  ATCCGACCCTTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCTAAAGAAGAGGCGTTCGGCCCCCTCCCCCCGCCGTTCCGCTTTAAAGATGA                                                                             <  SRR4302540.6913253/94‑1 (MQ=60)

                  AGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGA                                                                             >  SRR4302540.7442370/1‑94 (MQ=60)

                  AGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGA                                                                             <  SRR4302540.3153103/94‑1 (MQ=60)

                  AGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGA                                                                             >  SRR4302540.901015/1‑94 (MQ=60)

                   cccgcccatcCTGGTGGACCTTCCGGCCACCGCCAAAGTCGCGAAAGAAGAGCCGTTCCGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAA                                                                            <  SRR4302540.3338989/84‑1 (MQ=58)

                   CCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAA                                                                            <  SRR4302540.3338995/94‑1 (MQ=60)

                    CCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAG                                                                           <  SRR4302540.413947/94‑1 (MQ=60)

                    CCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAG                                                                           >  SRR4302540.6845802/1‑94 (MQ=60)

                    CCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAG                                                                           >  SRR4302540.1754937/1‑94 (MQ=60)

                    CCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAG                                                                           <  SRR4302540.7096528/94‑1 (MQ=60)

                     CGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGC                                                                          >  SRR4302540.2839207/1‑94 (MQ=60)

                     CGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGC                                                                          >  SRR4302540.2511297/1‑94 (MQ=60)

                      GACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCT                                                                         <  SRR4302540.7722650/94‑1 (MQ=60)

                      GACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCT                                                                         <  SRR4302540.3684623/94‑1 (MQ=60)

                      GACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCT                                                                         >  SRR4302540.4793551/1‑94 (MQ=60)

                      CACCATTCTGGTGGACGTTCCGGCCAACGCCAAGGGGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCT                                                                         <  SRR4302540.2131050/94‑1 (MQ=60)

                          ATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATG                                                                     >  SRR4302540.6176838/1‑94 (MQ=60)

                          ATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATG                                                                     >  SRR4302540.10105504/1‑94 (MQ=60)

                          ATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATG                                                                     <  SRR4302540.7753150/94‑1 (MQ=60)

                              TGGTGGACGTTCGGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGAT                                                                 <  SRR4302540.69833/94‑1 (MQ=60)

                              TGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACTTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGAT                                                                 <  SRR4302540.731403/94‑1 (MQ=60)

                              TGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGAT                                                                 <  SRR4302540.3649201/94‑1 (MQ=60)

                              TGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGAT                                                                 <  SRR4302540.5572715/94‑1 (MQ=60)

                              TGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGAT                                                                 >  SRR4302540.6265050/1‑94 (MQ=60)

                               GGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATT                                                                >  SRR4302540.4789859/1‑94 (MQ=60)

                               GGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATT                                                                >  SRR4302540.9571295/1‑94 (MQ=60)

                                GTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTG                                                               <  SRR4302540.5079421/94‑1 (MQ=60)

                                 TGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGC                                                              >  SRR4302540.7071307/1‑94 (MQ=60)

                                 TGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGC                                                              <  SRR4302540.1599944/94‑1 (MQ=60)

                                 TGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGC                                                              >  SRR4302540.1572760/1‑94 (MQ=60)

                                  GGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCG                                                             >  SRR4302540.5376315/1‑94 (MQ=60)

                                  GGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCG                                                             >  SRR4302540.6111573/1‑94 (MQ=60)

                                     CGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAA                                                          >  SRR4302540.8141001/1‑94 (MQ=60)

                                      GTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAG                                                         >  SRR4302540.7322818/1‑94 (MQ=60)

                                      GTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAG                                                         >  SRR4302540.6909707/1‑94 (MQ=60)

                                      GTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAG                                                         >  SRR4302540.7322819/1‑94 (MQ=60)

                                      GTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAG                                                         >  SRR4302540.2913654/1‑94 (MQ=60)

                                      GTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAG                                                         >  SRR4302540.870414/1‑94 (MQ=60)

                                         CCGGTCAACGCCAAAGTGTCGAAAGAACAGTCGTTCGGCCTCCGCGCCCCGCCTTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCA                                                      <  SRR4302540.9330519/94‑1 (MQ=60)

                                           gacaaccgccagagtgtcaaaaaacGAGACTTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAAT                                                    <  SRR4302540.3455942/69‑1 (MQ=60)

                                           GGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAAT                                                    <  SRR4302540.2716621/94‑1 (MQ=60)

                                           GGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAAT                                                    <  SRR4302540.5335707/94‑1 (MQ=60)

                                           GGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCACGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAAT                                                    <  SRR4302540.3248485/94‑1 (MQ=60)

                                           GCCCAACGCCAAAGTGTCGAAAGAAGAGCCGTCCGCCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAAT                                                    <  SRR4302540.5450381/94‑1 (MQ=60)

                                            GCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATG                                                   >  SRR4302540.959515/1‑94 (MQ=60)

                                             CCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGA                                                  >  SRR4302540.2987181/1‑94 (MQ=60)

                                               cactccaaatggtggaaaaaatAGACGTTCGGCCCCCTCTCCCCGCCTTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACA                                                <  SRR4302540.3889972/72‑1 (MQ=60)

                                               AACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACA                                                >  SRR4302540.4438094/1‑94 (MQ=60)

                                               AACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACA                                                <  SRR4302540.3174120/94‑1 (MQ=60)

                                               AACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACA                                                >  SRR4302540.3731960/1‑94 (MQ=60)

                                               AACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACA                                                <  SRR4302540.1633022/94‑1 (MQ=60)

                                               AACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACA                                                >  SRR4302540.7067811/1‑94 (MQ=60)

                                                ACGCAAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACAC                                               <  SRR4302540.314346/94‑1 (MQ=60)

                                                 CGCCAAAGTGTCAAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACC                                              <  SRR4302540.1044904/94‑1 (MQ=60)

                                                  GCCAAAGTGTAGAAAGAAGAGCCGTTCGCCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCG                                             <  SRR4302540.4604933/94‑1 (MQ=60)

                                                  CCCAAAGTGTCGAAAGAAGAGACGTTCGCCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCG                                             <  SRR4302540.800067/94‑1 (MQ=60)

                                                   CCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGA                                            >  SRR4302540.1398979/1‑94 (MQ=60)

                                                   CCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGA                                            <  SRR4302540.60384/94‑1 (MQ=60)

                                                      AAGTGTCGAAAGAAGAGGCGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTT                                         <  SRR4302540.5918431/94‑1 (MQ=60)

                                                       AGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTT                                        >  SRR4302540.8728942/1‑94 (MQ=60)

                                                       AGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTT                                        >  SRR4302540.5843798/1‑94 (MQ=60)

                                                        GTGTAGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTG                                       <  SRR4302540.1105279/94‑1 (MQ=60)

                                                         TGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGG                                      >  SRR4302540.5705528/1‑94 (MQ=60)

                                                         TGTCGAAAAAAGAGACGTCCGCCCCCCCCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGG                                      <  SRR4302540.598138/94‑1 (MQ=60)

                                                          GTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGC                                     >  SRR4302540.9404648/1‑94 (MQ=60)

                                                           TCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCC                                    >  SRR4302540.9897791/1‑94 (MQ=60)

                                                            CGAAAGAAGAGCCGTTCGCCCCCCTCCCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCT                                   <  SRR4302540.4262621/94‑1 (MQ=60)

                                                            CGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCT                                   >  SRR4302540.2812415/1‑94 (MQ=60)

                                                            CGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCT                                   >  SRR4302540.8043438/1‑94 (MQ=60)

                                                            CGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCT                                   >  SRR4302540.2297011/1‑94 (MQ=60)

                                                            CGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCT                                   <  SRR4302540.5200838/94‑1 (MQ=60)

                                                             gaagaaagacacgttggcccccccccCCCCGCCGTTCCGTTTTAAAGATGAAGCTGATGTGATTGCGAAAGCCAATGACCCCGAGTTTGGCCTT                                  <  SRR4302540.1989349/68‑1 (MQ=60)

                                                             GAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGCTTGGCCTT                                  >  SRR4302540.6293953/1‑94 (MQ=60)

                                                              AAAAAAGAGACTTTCGCCCCCCCCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTG                                 <  SRR4302540.353267/94‑1 (MQ=60)

                                                               AAGAAGAGACTTTCGCCCCCCTCCCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGC                                <  SRR4302540.7712622/94‑1 (MQ=60)

                                                               AAGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGC                                >  SRR4302540.2767877/1‑94 (MQ=60)

                                                               AAGAAAGGACGTCCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGC                                <  SRR4302540.557049/94‑1 (MQ=60)

                                                                aaaagagcctttcgcccccccccCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCC                               <  SRR4302540.3410717/71‑1 (MQ=60)

                                                                AGAAGGGACGTTGGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCC                               <  SRR4302540.1554850/94‑1 (MQ=60)

                                                                AGAAGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCC                               >  SRR4302540.8168705/1‑94 (MQ=60)

                                                                AGAAGAGACGTGCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCC                               <  SRR4302540.5369491/94‑1 (MQ=60)

                                                                AGAAGAGACGTGCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCC                               <  SRR4302540.8120021/94‑1 (MQ=60)

                                                                AAAAGAGACGTTCGCCCCCCTGGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCC                               <  SRR4302540.10056773/94‑1 (MQ=60)

                                                                   AGAGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCC                            >  SRR4302540.9681287/1‑94 (MQ=60)

                                                                     AGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGGGCAAGCCAATGACACCGAGTTTGGCCTTGCGGCCTA                          >  SRR4302540.8389049/1‑94 (MQ=60)

                                                                     AGACGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTA                          >  SRR4302540.7414749/1‑94 (MQ=60)

                                                                      gagtttcgcCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTAT                         <  SRR4302540.4823880/85‑1 (MQ=60)

                                                                      acCGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTAT                         <  SRR4302540.2872714/92‑1 (MQ=60)

                                                                        CGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCCCCTATTT                       >  SRR4302540.2139572/1‑94 (MQ=60)

                                                                        CGTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTCGTCCTGCCGCCTATTT                       >  SRR4302540.5674771/1‑94 (MQ=60)

                                                                         GTTCGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTC                      >  SRR4302540.9863862/1‑94 (MQ=60)

                                                                          TTGGGCCCCCCCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCT                     <  SRR4302540.5452483/94‑1 (MQ=60)

                                                                            CGGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTAC                   <  SRR4302540.4462427/94‑1 (MQ=60)

                                                                            CCGCCCCCTCGCCCCGCCGGTCCGCTTTAAAGATGAAGTTGATGTGATTGCGCAAGCCAATGACACTGAGTTTGGCCTTGCCGCCTATTTCTAC                   <  SRR4302540.5152198/94‑1 (MQ=60)

                                                                             GGCCCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACG                  >  SRR4302540.3126751/1‑94 (MQ=60)

                                                                                CCCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCC               <  SRR4302540.2236460/94‑1 (MQ=60)

                                                                                 CCCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCG              >  SRR4302540.8615522/1‑94 (MQ=60)

                                                                                  CCTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGT             >  SRR4302540.607207/1‑94 (MQ=60)

                                                                                   CTCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTG            >  SRR4302540.455808/1‑94 (MQ=60)

                                                                                    TCTCCCCGCTGTTCCGCTTGAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTGA           <  SRR4302540.2824475/94‑1 (MQ=60)

                                                                                    TCGCCCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTGA           >  SRR4302540.9924814/1‑94 (MQ=60)

                                                                                       CCCCCCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTGCGCCCGTGATTT        <  SRR4302540.678958/94‑1 (MQ=60)

                                                                                        CCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTGATTTA       >  SRR4302540.8861272/1‑94 (MQ=60)

                                                                                        CCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTGATTTA       >  SRR4302540.3739761/1‑94 (MQ=60)

                                                                                        CCCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTGATTTA       >  SRR4302540.4083495/1‑94 (MQ=60)

                                                                                         CCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTGATTTAA      >  SRR4302540.4554776/1‑94 (MQ=60)

                                                                                         CCGCCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTGATTTAA      >  SRR4302540.6865901/1‑94 (MQ=60)

                                                                                            CCGTTCCGTTTTAAAGATGAAGCTAAGGTGATTGCGAAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTGATTTAAGCC   <  SRR4302540.739560/94‑1 (MQ=60)

                                                                                            CCGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTGATTTAAGCC   >  SRR4302540.5780608/1‑94 (MQ=60)

                                                                                             CGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTGATTTAAGCCG  >  SRR4302540.201478/1‑94 (MQ=60)

                                                                                             CGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTGATTTAAGCCG  >  SRR4302540.594844/1‑94 (MQ=60)

                                                                                             CGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTGATTTAAGCCG  >  SRR4302540.1086385/1‑94 (MQ=60)

                                                                                             CGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTGATTTAAGCCG  >  SRR4302540.4243096/1‑94 (MQ=60)

GCGGCGGCAACTTCTTCCAGCCGACCATTCTGGTGGACGTTCCGGCCAACGCCAAAGTGTCGAAAGAAGAGACGTTCGGCCCCCTCGCCCCGCTGTTCCGCTTTAAAGATGAAGCTGATGTGATTGCGCAAGCCAATGACACCGAGTTTGGCCTTGCCGCCTATTTCTACGCCCGTGATTTAAGCCG  >  NC_000913/2790381‑2790567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`