Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,811,545	G→A	A303A (GCG→GCA)	emrB →	EmrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,811,545	0	G	A	89.9%	707.9 / 43.6	247	A303A (GCG→GCA)	emrB	EmrB
Reads supporting (aligned to +/- strand): ref base G (9/16); new base A (101/121); total (110/137)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.79e-01

Only 100 of 259 total aligned reads displayed.

GCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATAT  >  NC_000913/2811454‑2811634

gctgctTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGa                                                                                          >  2:1427146/1‑93 (MQ=255)

 ctgctTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAAc                                                                                         <  1:3264857/93‑1 (MQ=255)

   gctTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCt                                                                                       >  2:5005730/1‑93 (MQ=255)

   gctTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCt                                                                                       >  2:5447793/1‑93 (MQ=255)

     ttGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTgg                                                                                     >  2:1484426/1‑93 (MQ=255)

     ttGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTgg                                                                                     <  1:4381830/93‑1 (MQ=255)

      tgtgTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTggg                                                                                    >  2:9609075/1‑93 (MQ=255)

      tgtgTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTggg                                                                                    >  2:836572/1‑93 (MQ=255)

      tgtgTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTggg                                                                                    >  2:8343107/1‑93 (MQ=255)

       gtgtATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGc                                                                                   <  2:6628610/93‑1 (MQ=255)

          tATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAgg                                                                                <  1:6373799/93‑1 (MQ=255)

           aTCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGt                                                                               >  2:4822179/1‑93 (MQ=255)

           aTCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGt                                                                               >  2:7862235/1‑93 (MQ=255)

           aTCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGt                                                                               <  2:8683026/93‑1 (MQ=255)

            tCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGtt                                                                              <  1:7904373/93‑1 (MQ=255)

            tCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGtt                                                                              >  2:2986105/1‑93 (MQ=255)

             cAGCCTGGCGTACATGCTCTACTTCGGCGCTATTGTTCTGCTGCTGCAGTGGCTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGttt                                                                             <  2:4445708/93‑1 (MQ=255)

                 cTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGcc                                                                         >  1:8549750/1‑93 (MQ=255)

                 cTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGcc                                                                         <  1:4256295/93‑1 (MQ=255)

                 cTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGcc                                                                         <  1:9117256/93‑1 (MQ=255)

                  tCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCct                                                                        <  2:6112651/93‑1 (MQ=255)

                  tCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCct                                                                        >  1:2876672/1‑93 (MQ=255)

                   cgcgTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCctc                                                                       >  2:9316742/1‑93 (MQ=255)

                    gcgTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCctct                                                                      >  2:9282594/1‑93 (MQ=255)

                    gcctataTGCTCTACTTCGGCACTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAGCCTGGGCAGGTTTGGCctct                                                                      <  2:8041490/90‑1 (MQ=255)

                     cgTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTg                                                                     <  1:6061354/93‑1 (MQ=255)

                      gTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTgc                                                                    <  1:8006682/93‑1 (MQ=255)

                      gTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTgc                                                                    <  1:5662674/93‑1 (MQ=255)

                       tataTGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTgcg                                                                   >  2:2828647/1‑93 (MQ=255)

                        atatGCTCTACTTCGGCGCTATGGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTgcgc                                                                  <  2:6872130/93‑1 (MQ=255)

                        atatGCTCTACTTCGGCGCTAGTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTgcgc                                                                  <  2:2207736/93‑1 (MQ=255)

                         tatGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGcc                                                                 >  1:710800/1‑93 (MQ=255)

                         tatGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGcc                                                                 >  2:6902237/1‑93 (MQ=255)

                          atGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCGGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCg                                                                >  1:1424289/1‑93 (MQ=255)

                             gtctGCTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTa                                                             <  1:1640777/92‑1 (MQ=255)

                             ctctACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGtg                                                             >  2:3057762/1‑92 (MQ=255)

                             ctctACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGtg                                                             >  2:7455756/1‑92 (MQ=255)

                             ctctACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTa                                                             <  2:5242961/93‑1 (MQ=255)

                               ctACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAgg                                                           <  1:9653029/93‑1 (MQ=255)

                                     cGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGattat                                                     >  1:9173048/1‑93 (MQ=255)

                                          cTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGCTTGGCCTCTGCGCCGGTAGGGATTATTCCgg                                                >  2:1073689/1‑93 (MQ=255)

                                          cTATTGTTCTGCCGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCgg                                                >  2:7086029/1‑93 (MQ=255)

                                           tATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGTGCCGGTAGGGATTATTCCGGt                                               <  1:7597002/93‑1 (MQ=255)

                                             ttGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGa                                             <  1:8433700/93‑1 (MQ=255)

                                               gTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATc                                           >  2:8936902/1‑93 (MQ=255)

                                                ttCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATcc                                          >  1:372990/1‑93 (MQ=255)

                                                ttCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATcc                                          >  1:4085262/1‑93 (MQ=255)

                                                ttCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATcc                                          >  2:7685558/1‑93 (MQ=255)

                                                  ctgctgCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTg                                        >  1:6171602/1‑93 (MQ=255)

                                                  ctgctgCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTg                                        <  1:5848868/93‑1 (MQ=255)

                                                   tgctgcCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGGCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGt                                       <  1:4821746/93‑1 (MQ=255)

                                                   tgctgcCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGt                                       <  1:9398785/93‑1 (MQ=255)

                                                   tgctgcCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGt                                       <  2:4780253/93‑1 (MQ=255)

                                                    gctgcCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTc                                      >  2:9611922/1‑93 (MQ=255)

                                                      tgcCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGc                                    >  2:4375331/1‑93 (MQ=255)

                                                      tgcCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGc                                    >  1:4110466/1‑93 (MQ=255)

                                                      tgcCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGc                                    >  2:4539549/1‑93 (MQ=255)

                                                      tgcCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCCCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGc                                    <  1:3076773/93‑1 (MQ=255)

                                                       gcCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGcc                                   <  2:567825/93‑1 (MQ=255)

                                                       gcCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGcc                                   >  1:1990194/1‑93 (MQ=255)

                                                        ccGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCg                                  >  1:5537407/1‑93 (MQ=255)

                                                          gCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCggt                                >  2:7146932/1‑91 (MQ=255)

                                                          gCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGAt                                <  2:6347712/93‑1 (MQ=255)

                                                             gttgttGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTAt                             >  1:35704/1‑93 (MQ=255)

                                                             gttgttGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTAt                             >  1:6963789/1‑93 (MQ=255)

                                                             gttgttGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTAt                             >  2:6683401/1‑93 (MQ=255)

                                                             gttgttGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTAt                             >  2:3885641/1‑93 (MQ=255)

                                                              ttgttgCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATc                            >  1:8782436/1‑93 (MQ=255)

                                                              ttgttgCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATc                            <  1:89895/93‑1 (MQ=255)

                                                                gttgCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCgg                          >  1:4681992/1‑93 (MQ=255)

                                                                gttgCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCgg                          <  2:1992183/93‑1 (MQ=255)

                                                                gttgCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCgg                          >  1:3152548/1‑93 (MQ=255)

                                                                 ttgCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGc                         <  2:3392941/93‑1 (MQ=255)

                                                                 gtgCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGc                         <  1:5283875/92‑1 (MQ=255)

                                                                  tgCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGcc                        <  2:6458690/93‑1 (MQ=255)

                                                                   gCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCg                       >  1:8710668/1‑93 (MQ=255)

                                                                    cAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGc                      <  2:7051604/93‑1 (MQ=255)

                                                                      ggaggTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCtt                    >  1:2493801/1‑93 (MQ=255)

                                                                      ggaggTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCtt                    >  1:1010808/1‑93 (MQ=255)

                                                                       gaggTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTc                   >  2:9782214/1‑93 (MQ=255)

                                                                       gaggTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTc                   <  1:6395115/93‑1 (MQ=255)

                                                                       gaggTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTc                   <  2:3510029/93‑1 (MQ=255)

                                                                         ggTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTcgc                 <  2:7195592/93‑1 (MQ=255)

                                                                           tCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCgcgc               <  2:6203071/93‑1 (MQ=255)

                                                                           tCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCgcgc               >  2:1017441/1‑93 (MQ=255)

                                                                              aCGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATa            >  1:7298682/1‑93 (MQ=255)

                                                                              aCGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATa            <  1:3424020/93‑1 (MQ=255)

                                                                                ggatACACGGCGACCTGGGAAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATaaa          <  2:9206920/90‑1 (MQ=255)

                                                                                ggTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATaaa          <  1:3491347/93‑1 (MQ=255)

                                                                                ggTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATaaa          <  2:4194351/93‑1 (MQ=255)

                                                                                ggTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATaaa          <  2:2532793/93‑1 (MQ=255)

                                                                                 gTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAAc         >  1:2258484/1‑93 (MQ=255)

                                                                                 gTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAAc         <  1:2035984/93‑1 (MQ=255)

                                                                                  ttACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACt        <  1:4591456/93‑1 (MQ=255)

                                                                                  ttACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACt        <  2:6312604/93‑1 (MQ=255)

                                                                                   tACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTg       <  2:5791775/93‑1 (MQ=255)

                                                                                    acacGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTgg      <  1:1750596/93‑1 (MQ=255)

                                                                                      acGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGat    <  2:1811144/93‑1 (MQ=255)

                                                                                       cGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGata   <  1:5249082/93‑1 (MQ=255)

                                                                                       cGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGata   <  1:2804899/93‑1 (MQ=255)

GCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATAT  >  NC_000913/2811454‑2811634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATATGCGG  >  NC_000913/2811452‑2811638

CGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCA                                                                                               <  SRR4302540.519219/94‑1 (MQ=60)

CGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCA                                                                                               <  SRR4302540.519218/94‑1 (MQ=60)

CGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTACTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCA                                                                                               <  SRR4302540.857128/94‑1 (MQ=60)

 GGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAA                                                                                              >  SRR4302540.5837863/1‑94 (MQ=60)

  GCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGCGGTCTACGGTTACACGGCAAC                                                                                             >  SRR4302540.3270791/1‑94 (MQ=60)

  GCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAAC                                                                                             <  SRR4302540.5084723/94‑1 (MQ=60)

   CTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACC                                                                                            <  SRR4302540.3300479/94‑1 (MQ=60)

    TGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCT                                                                                           >  SRR4302540.5618494/1‑94 (MQ=60)

     GCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTG                                                                                          <  SRR4302540.3130966/94‑1 (MQ=60)

       TTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGG                                                                                        <  SRR4302540.4429413/94‑1 (MQ=60)

         GTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCA                                                                                      <  SRR4302540.3781390/94‑1 (MQ=60)

          TGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAG                                                                                     <  SRR4302540.2051896/94‑1 (MQ=60)

            TATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGT                                                                                   <  SRR4302540.6444763/94‑1 (MQ=60)

            TATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGT                                                                                   >  SRR4302540.684260/1‑94 (MQ=60)

             ATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTT                                                                                  >  SRR4302540.1958411/1‑94 (MQ=60)

              TCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTT                                                                                 <  SRR4302540.9280227/94‑1 (MQ=60)

              TCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTT                                                                                 <  SRR4302540.7996561/94‑1 (MQ=60)

              TCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTT                                                                                 <  SRR4302540.745243/94‑1 (MQ=60)

               CAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTG                                                                                <  SRR4302540.3250041/94‑1 (MQ=60)

                AGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGG                                                                               <  SRR4302540.9368393/94‑1 (MQ=60)

                AGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGG                                                                               <  SRR4302540.6165592/94‑1 (MQ=60)

                  CCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCC                                                                             >  SRR4302540.8649067/1‑94 (MQ=60)

                  CCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTGGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCC                                                                             <  SRR4302540.9755306/94‑1 (MQ=60)

                   CTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCT                                                                            <  SRR4302540.4302599/94‑1 (MQ=60)

                   CTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCT                                                                            >  SRR4302540.6371873/1‑94 (MQ=60)

                   CTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCT                                                                            >  SRR4302540.2907947/1‑94 (MQ=60)

                   CTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCT                                                                            <  SRR4302540.9226845/94‑1 (MQ=60)

                    TCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTC                                                                           <  SRR4302540.7130814/94‑1 (MQ=60)

                     CGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCT                                                                          >  SRR4302540.212328/1‑94 (MQ=60)

                      GCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTG                                                                         >  SRR4302540.2161400/1‑94 (MQ=60)

                       CGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGC                                                                        <  SRR4302540.4700662/94‑1 (MQ=60)

                       CGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGC                                                                        <  SRR4302540.6128720/94‑1 (MQ=60)

                        GTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCG                                                                       <  SRR4302540.5725028/94‑1 (MQ=60)

                        GTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCG                                                                       <  SRR4302540.8099853/94‑1 (MQ=60)

                        GTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCG                                                                       <  SRR4302540.3506246/94‑1 (MQ=60)

                         TATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGC                                                                      <  SRR4302540.8155175/94‑1 (MQ=60)

                          ATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCC                                                                     <  SRR4302540.4626040/94‑1 (MQ=60)

                          ATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCC                                                                     >  SRR4302540.7549620/1‑94 (MQ=60)

                          ATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCC                                                                     >  SRR4302540.718436/1‑94 (MQ=60)

                           TATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCGGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCG                                                                    >  SRR4302540.1439709/1‑94 (MQ=60)

                           TATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCG                                                                    >  SRR4302540.2382152/1‑94 (MQ=60)

                            ATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGG                                                                   <  SRR4302540.7372651/94‑1 (MQ=60)

                             TGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGT                                                                  >  SRR4302540.1130548/1‑94 (MQ=60)

                             TGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGT                                                                  >  SRR4302540.10047540/1‑94 (MQ=60)

                             TGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGT                                                                  <  SRR4302540.4873803/94‑1 (MQ=60)

                               gtctgCTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAG                                                                <  SRR4302540.1658709/89‑1 (MQ=60)

                               CTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAG                                                                <  SRR4302540.3729047/94‑1 (MQ=60)

                               CTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAG                                                                >  SRR4302540.8755373/1‑94 (MQ=60)

                                TCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGG                                                               <  SRR4302540.5900725/94‑1 (MQ=60)

                                TCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGG                                                               >  SRR4302540.9251353/1‑94 (MQ=60)

                                 gaacttcggctatactgttctgttaCCGCAGGTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGG                                                              <  SRR4302540.9766684/69‑1 (MQ=60)

                                 CTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGG                                                              <  SRR4302540.9766683/94‑1 (MQ=60)

                                  TACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGA                                                             <  SRR4302540.1505087/94‑1 (MQ=60)

                                   TCTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGAT                                                            <  SRR4302540.3584999/94‑1 (MQ=60)

                                    CTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATT                                                           >  SRR4302540.937742/1‑94 (MQ=60)

                                      TCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTAT                                                         <  SRR4302540.8084028/94‑1 (MQ=60)

                                      TCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTAT                                                         >  SRR4302540.9283082/1‑94 (MQ=60)

                                      TCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTAT                                                         <  SRR4302540.8007069/94‑1 (MQ=60)

                                       tcgggtctgTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATT                                                        <  SRR4302540.6431516/85‑1 (MQ=60)

                                       CGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATT                                                        <  SRR4302540.6431518/94‑1 (MQ=60)

                                       CGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATT                                                        >  SRR4302540.8816376/1‑94 (MQ=60)

                                         GCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCC                                                      >  SRR4302540.3117551/1‑94 (MQ=60)

                                             gtttgtgcgggtgccggtggtcgtgcaagagctagACGGTTACACGACAACCTTGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTG                                                  <  SRR4302540.8141907/59‑1 (MQ=60)

                                             TATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTG                                                  <  SRR4302540.8929378/94‑1 (MQ=60)

                                             TATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGTGCCGGTAGGGATTATTCCGGTG                                                  <  SRR4302540.7682352/94‑1 (MQ=60)

                                             TATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTG                                                  <  SRR4302540.6806959/94‑1 (MQ=60)

                                               TTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTGCGGTTACACGGCACCCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGAT                                                <  SRR4302540.2881966/94‑1 (MQ=60)

                                               TTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGAT                                                <  SRR4302540.8531610/94‑1 (MQ=60)

                                                TGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATC                                               <  SRR4302540.5575948/94‑1 (MQ=60)

                                                TGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATC                                               <  SRR4302540.8525997/94‑1 (MQ=60)

                                                 GTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGTTGATCC                                              >  SRR4302540.4129320/1‑94 (MQ=60)

                                                 GTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCC                                              >  SRR4302540.376845/1‑94 (MQ=60)

                                                 GTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCC                                              >  SRR4302540.4129318/1‑94 (MQ=60)

                                                   TCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTG                                            <  SRR4302540.9849512/94‑1 (MQ=60)

                                                   TCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTG                                            >  SRR4302540.6240361/1‑94 (MQ=60)

                                                   TCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGGTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTG                                            >  SRR4302540.3868145/1‑94 (MQ=60)

                                                    CTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGT                                           <  SRR4302540.2351261/94‑1 (MQ=60)

                                                    CTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGT                                           <  SRR4302540.5913555/94‑1 (MQ=60)

                                                     TGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTC                                          <  SRR4302540.8591376/94‑1 (MQ=60)

                                                     TGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTC                                          <  SRR4302540.4787787/94‑1 (MQ=60)

                                                     TGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGGCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTC                                          <  SRR4302540.4873362/94‑1 (MQ=60)

                                                     TGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTC                                          <  SRR4302540.9206295/94‑1 (MQ=60)

                                                     TGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTC                                          <  SRR4302540.9510605/94‑1 (MQ=60)

                                                     TGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTC                                          <  SRR4302540.5257212/94‑1 (MQ=60)

                                                      gtgaCCGCAGTTGTTGCAGGAGGTTTACGGTTACTCGGCAGCCTGGGCAGGTTTGGGCTCCGCGCCGGTAGGGATTATTCCGGTGATCCTGTCG                                         <  SRR4302540.8681181/90‑1 (MQ=60)

                                                      GCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGAGCCTGTCC                                         >  SRR4302540.7067514/1‑94 (MQ=60)

                                                      GCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCG                                         <  SRR4302540.3382374/94‑1 (MQ=60)

                                                       CTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGC                                        >  SRR4302540.5957465/1‑94 (MQ=60)

                                                       CTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGC                                        >  SRR4302540.4154836/1‑94 (MQ=60)

                                                        TGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCC                                       >  SRR4302540.1505295/1‑94 (MQ=60)

                                                        TGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCC                                       <  SRR4302540.6001810/94‑1 (MQ=60)

                                                        TGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCC                                       >  SRR4302540.2011764/1‑94 (MQ=60)

                                                        TGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCCCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCC                                       <  SRR4302540.3110530/94‑1 (MQ=60)

                                                         GCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCG                                      >  SRR4302540.5598395/1‑94 (MQ=60)

                                                           CGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGAT                                    >  SRR4302540.1818855/1‑94 (MQ=60)

                                                              AGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTAT                                 >  SRR4302540.7041232/1‑94 (MQ=60)

                                                              AGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTAT                                 <  SRR4302540.4427129/94‑1 (MQ=60)

                                                              AGTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTAT                                 >  SRR4302540.36076/1‑94 (MQ=60)

                                                               GTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATC                                >  SRR4302540.8884105/1‑94 (MQ=60)

                                                               GTTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATC                                <  SRR4302540.5572241/94‑1 (MQ=60)

                                                                TTGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCG                               <  SRR4302540.90841/94‑1 (MQ=60)

                                                                 TGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGG                              >  SRR4302540.4732079/1‑94 (MQ=60)

                                                                 TGTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGG                              >  SRR4302540.3186870/1‑94 (MQ=60)

                                                                  GTTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGC                             >  SRR4302540.4253558/1‑94 (MQ=60)

                                                                   GTGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCC                            <  SRR4302540.5341614/94‑1 (MQ=60)

                                                                    TGCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCG                           >  SRR4302540.8811785/1‑94 (MQ=60)

                                                                     GCAGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGC                          >  SRR4302540.448720/1‑94 (MQ=60)

                                                                       AGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTT                        >  SRR4302540.2520672/1‑94 (MQ=60)

                                                                       AGGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTT                        >  SRR4302540.1021057/1‑94 (MQ=60)

                                                                        GGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTC                       >  SRR4302540.3497267/1‑94 (MQ=60)

                                                                        GGAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTC                       <  SRR4302540.5315358/94‑1 (MQ=60)

                                                                         GAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCG                      <  SRR4302540.9022216/94‑1 (MQ=60)

                                                                         GAGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCG                      <  SRR4302540.6466302/94‑1 (MQ=60)

                                                                          AGGTCTACGGTTATACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGC                     >  SRR4302540.7756957/1‑94 (MQ=60)

                                                                          AGGTCTACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGC                     >  SRR4302540.6465645/1‑94 (MQ=60)

                                                                               TACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATA                >  SRR4302540.7380146/1‑94 (MQ=60)

                                                                                ACGGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAA               <  SRR4302540.3461247/94‑1 (MQ=60)

                                                                                  GGTTGCACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTAATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAAC             <  SRR4302540.5735233/94‑1 (MQ=60)

                                                                                  GGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAAC             <  SRR4302540.3529371/94‑1 (MQ=60)

                                                                                  GGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAAC             >  SRR4302540.5774621/1‑94 (MQ=60)

                                                                                  GGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAAC             >  SRR4302540.2282724/1‑94 (MQ=60)

                                                                                  GGTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGGGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAAG             >  SRR4302540.2559432/1‑94 (MQ=60)

                                                                                   GTTACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACT            <  SRR4302540.2057939/94‑1 (MQ=60)

                                                                                    TTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTG           <  SRR4302540.4640873/94‑1 (MQ=60)

                                                                                      ACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGA         <  SRR4302540.1769558/94‑1 (MQ=60)

                                                                                      ACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGA         <  SRR4302540.1769559/94‑1 (MQ=60)

                                                                                      ACACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGA         >  SRR4302540.4762504/1‑94 (MQ=60)

                                                                                       CACGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGAT        <  SRR4302540.8694047/94‑1 (MQ=60)

                                                                                         CGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATAT      <  SRR4302540.2835193/94‑1 (MQ=60)

                                                                                         CGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATAT      <  SRR4302540.3670953/94‑1 (MQ=60)

                                                                                         CGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATAT      <  SRR4302540.2835195/94‑1 (MQ=60)

                                                                                         CGGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATAT      <  SRR4302540.5306335/94‑1 (MQ=60)

                                                                                          GGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATATG     >  SRR4302540.2976057/1‑94 (MQ=60)

                                                                                          GGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATATG     <  SRR4302540.3016187/94‑1 (MQ=60)

                                                                                          GGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATATG     <  SRR4302540.4250511/94‑1 (MQ=60)

                                                                                          GGCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATATG     >  SRR4302540.2976058/1‑94 (MQ=60)

                                                                                           GCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATATGC    <  SRR4302540.3857698/94‑1 (MQ=60)

                                                                                           GCAACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATATGC    <  SRR4302540.10001266/94‑1 (MQ=60)

                                                                                             AACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATATGCGG  >  SRR4302540.2045319/1‑94 (MQ=60)

                                                                                             AACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATATGCGG  >  SRR4302540.835824/1‑94 (MQ=60)

CGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATATGCGG  >  NC_000913/2811452‑2811638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`