Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,847,139	A→G	G42G (GGT→GGC)	hycC ←	membrane‑spanning protein of hydrogenase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,847,139	0	A	G	99.7%	1003.0 / NA	305	G42G (GGT→GGC)	hycC	membrane‑spanning protein of hydrogenase 3
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (151/153); minor base C (0/1); total (151/154)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 314 total aligned reads displayed.

TTGCACATCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTACCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACAAACCA  >  NC_000913/2847049‑2847228

   cacaTCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAAc                                                                                      <  2:4543251/93‑1 (MQ=255)

    ccaTCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAAcc                                                                                     <  1:2960409/92‑1 (MQ=255)

    acaTCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAAcc                                                                                     >  1:8030868/1‑93 (MQ=255)

      aTCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACcgc                                                                                   >  2:1847206/1‑93 (MQ=255)

      aTCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACcgc                                                                                   >  2:2053078/1‑93 (MQ=255)

      aTCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACcgc                                                                                   >  2:9471579/1‑93 (MQ=255)

      aTCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACcgc                                                                                   >  2:6392799/1‑93 (MQ=255)

       tCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACcgcg                                                                                  >  2:5201576/1‑93 (MQ=255)

       tCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACcgcg                                                                                  >  1:381954/1‑93 (MQ=255)

       tCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACcgcg                                                                                  >  2:730911/1‑93 (MQ=255)

         gTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCCACCGcgcc                                                                                >  2:9776426/1‑93 (MQ=255)

         gTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGcgcc                                                                                >  1:1832203/1‑93 (MQ=255)

         gTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGcgcc                                                                                >  1:9850400/1‑93 (MQ=255)

          tAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGcgccg                                                                               >  2:8276517/1‑93 (MQ=255)

          tAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGcgccg                                                                               >  2:2230497/1‑93 (MQ=255)

            gCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCgccgcc                                                                             >  2:7930816/1‑93 (MQ=255)

              ttACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCaa                                                                           <  1:4063742/93‑1 (MQ=255)

               tACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAAt                                                                          <  2:4375221/93‑1 (MQ=255)

                aCCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAAtt                                                                         <  1:2073645/93‑1 (MQ=255)

                  cAGCGACGGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTcc                                                                       >  1:6300726/1‑93 (MQ=255)

                   aGCGACAGCGCCCCGCTGACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCa                                                                      <  2:843399/93‑1 (MQ=255)

                    gCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAg                                                                     <  2:2471807/93‑1 (MQ=255)

                     cGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGc                                                                    <  2:4940609/93‑1 (MQ=255)

                       aCAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTa                                                                  <  2:6520429/93‑1 (MQ=255)

                       aCAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTa                                                                  >  1:3162217/1‑93 (MQ=255)

                       aCAGCGCACCGCTCACGCCAACCGCGCAAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTa                                                                  <  1:8276630/93‑1 (MQ=255)

                        cAGCGCCCCGCTCACTCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCTAATTCCAGCTAt                                                                 <  2:3484734/93‑1 (MQ=255)

                         aGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATc                                                                <  1:7666320/93‑1 (MQ=255)

                         aGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATc                                                                <  1:8594962/93‑1 (MQ=255)

                          gcgcACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATcc                                                               <  2:10019907/93‑1 (MQ=255)

                          gagcACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTACAGCTATcc                                                               >  2:1294469/3‑93 (MQ=255)

                           cgcACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCa                                                              <  1:5763331/93‑1 (MQ=255)

                               ccGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCa                                                          >  1:1108666/1‑93 (MQ=255)

                               ccGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCa                                                          <  2:8101486/93‑1 (MQ=255)

                                  cTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACtt                                                       >  2:7194409/1‑93 (MQ=255)

                                   tCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTa                                                      >  1:5267185/1‑93 (MQ=255)

                                     aCGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAAc                                                    >  2:397265/1‑93 (MQ=255)

                                       gCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGc                                                  <  2:6993100/93‑1 (MQ=255)

                                        ccAACCGCTCCGTTCAGTACAGGGAAGCCCGCGGCTTCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCt                                                 <  1:3239445/93‑1 (MQ=255)

                                        ccAACCGCGCGAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCt                                                 <  1:9171734/93‑1 (MQ=255)

                                        ccAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCCCTCAACGCt                                                 >  2:6263180/1‑93 (MQ=255)

                                           aCCGCCCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCtttt                                              <  1:7906024/93‑1 (MQ=255)

                                             cgcgCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTg                                            >  1:7244183/1‑93 (MQ=255)

                                              gcgcCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTga                                           <  2:2089967/93‑1 (MQ=255)

                                                 ccAGTCAGTACGGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTgaaaa                                        >  2:5865357/1‑93 (MQ=255)

                                                 ccAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTgaaaa                                        <  1:1561818/93‑1 (MQ=255)

                                                  cagtcagtACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTgaaaaa                                       >  2:2992189/1‑92 (MQ=255)

                                                  cagtcaggACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTgaaaag                                       <  1:6005326/93‑1 (MQ=255)

                                                   agtcagtACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTgaaaaga                                      <  2:4150223/93‑1 (MQ=255)

                                                    gtcagtACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTgaaaagaa                                     >  1:10045744/1‑93 (MQ=255)

                                                    gtcagtACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTgaaaagaa                                     <  1:9624725/93‑1 (MQ=255)

                                                     tcagtACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTgaaaggaaa                                    <  1:8912991/93‑1 (MQ=255)

                                                      cagtACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTgaaaagaaaa                                   <  1:551270/93‑1 (MQ=255)

                                                       agtCCAGTGAAGCCCGCGGCTGCCGTATACGGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGaaaagaaaag                                  <  1:3257196/93‑1 (MQ=255)

                                                       agtACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGaaaagaaaag                                  <  2:2284807/93‑1 (MQ=255)

                                                       agtACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGaaaagaaaag                                  <  1:7127894/93‑1 (MQ=255)

                                                       agtACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGaaaagaaaag                                  <  2:3116543/93‑1 (MQ=255)

                                                         tACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAagag                                <  2:6902385/93‑1 (MQ=255)

                                                          aCAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAgaga                               <  1:617609/93‑1 (MQ=255)

                                                          aCAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAgaga                               <  1:2607527/93‑1 (MQ=255)

                                                          aCAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAgaga                               <  2:4303009/93‑1 (MQ=255)

                                                           cAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGaa                              <  1:48105/93‑1 (MQ=255)

                                                           cAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGaa                              <  1:4941206/93‑1 (MQ=255)

                                                           cAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGaa                              <  1:4174498/93‑1 (MQ=255)

                                                            aGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGaaa                             <  2:385666/93‑1 (MQ=255)

                                                            aGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGaaa                             <  2:6154641/93‑1 (MQ=255)

                                                            aGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGaaa                             <  2:2853554/93‑1 (MQ=255)

                                                            aGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGaaa                             <  2:7604456/93‑1 (MQ=255)

                                                             gTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAAt                            <  2:5322914/93‑1 (MQ=255)

                                                             gTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAAt                            >  2:8701508/1‑93 (MQ=255)

                                                                aaGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGcc                         <  1:6378230/93‑1 (MQ=255)

                                                                 aGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCa                        <  2:1770634/93‑1 (MQ=255)

                                                                 aGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCa                        <  2:5628601/93‑1 (MQ=255)

                                                                  gCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAg                       >  2:2161257/1‑93 (MQ=255)

                                                                   cccGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGa                      >  2:8126855/1‑93 (MQ=255)

                                                                    ccGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGaa                     <  1:7334271/93‑1 (MQ=255)

                                                                       ctgCTGCCGTATACAGACTGCCAACCGCTCCGCTAATTCCGGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAg                  <  1:3598224/91‑1 (MQ=255)

                                                                        ggCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGACATGCCAGAACAgc                 >  2:3341720/1‑93 (MQ=255)

                                                                         gCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAgcg                <  1:8768658/93‑1 (MQ=255)

                                                                         gCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAgcg                >  1:5132624/1‑93 (MQ=255)

                                                                         gCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAgcg                >  1:936126/1‑93 (MQ=255)

                                                                          cTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAgcgg               >  1:1857035/1‑93 (MQ=255)

                                                                          cTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAgcgg               <  2:2370279/93‑1 (MQ=255)

                                                                           tGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAgcggc              >  2:4755549/1‑93 (MQ=255)

                                                                           tGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAgcggc              <  2:5102238/93‑1 (MQ=255)

                                                                            gCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAgcggcg             >  2:2751338/1‑93 (MQ=255)

                                                                              cGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAgcggcggc           <  2:8862692/93‑1 (MQ=255)

                                                                               gTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAgcggcggcg          >  1:6609295/1‑93 (MQ=255)

                                                                               gTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAgcggcggcg          >  2:8198216/1‑93 (MQ=255)

                                                                               gTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAgcggcggcg          <  1:233441/93‑1 (MQ=255)

                                                                                tataCAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGa         >  2:5765259/1‑93 (MQ=255)

                                                                                tataCAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGa         >  2:964726/1‑93 (MQ=255)

                                                                                  taCAGACTGCCAACCGCGCCGCCAATTCCAGCTATTCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGAGACa       >  2:3682250/1‑93 (MQ=255)

                                                                                  taCAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGACAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACa       >  1:3961186/1‑93 (MQ=255)

                                                                                  taCAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACa       <  1:10021292/93‑1 (MQ=255)

                                                                                  taCAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACa       >  2:9857231/1‑93 (MQ=255)

                                                                                  taCAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACa       >  2:1572872/1‑93 (MQ=255)

                                                                                       aCTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACAAAccc  >  1:8998281/1‑92 (MQ=255)

                                                                                       aCTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACAAAcca  <  2:9072806/93‑1 (MQ=255)

                                                                                       aCTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACAAAcca  <  1:2523247/93‑1 (MQ=255)

TTGCACATCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTACCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACAAACCA  >  NC_000913/2847049‑2847228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GATTTGCACATCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTACCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACAAACCACGCC  >  NC_000913/2847046‑2847232

GATTTGCACATCGTAGCTTACCAGCGACAGCGCCCCGATCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTG                                                                                               <  SRR4302540.929872/94‑1 (MQ=60)

GATTTGCACATCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTG                                                                                               <  SRR4302540.2952461/94‑1 (MQ=60)

 ATTTGCACATCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGC                                                                                              >  SRR4302540.7060845/1‑94 (MQ=60)

 ATTTGCACATCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGC                                                                                              >  SRR4302540.7679891/1‑94 (MQ=60)

 ATTTGCACATCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCCGCCGGATACAGACTGC                                                                                              >  SRR4302540.6619596/1‑94 (MQ=60)

  TTTGCACATCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCC                                                                                             <  SRR4302540.1174159/94‑1 (MQ=60)

  TTTGCACATCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCC                                                                                             >  SRR4302540.3795060/1‑94 (MQ=60)

   TTGCACATCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCA                                                                                            <  SRR4302540.3428486/94‑1 (MQ=60)

    TGCACATCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAA                                                                                           <  SRR4302540.3290143/94‑1 (MQ=60)

    TGCACATCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAA                                                                                           >  SRR4302540.6328543/1‑94 (MQ=60)

      CACATCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACC                                                                                         >  SRR4302540.8124237/1‑94 (MQ=60)

       CCATCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCG                                                                                        <  SRR4302540.2993046/94‑1 (MQ=60)

       ACATCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCG                                                                                        >  SRR4302540.9270117/1‑94 (MQ=60)

        CATCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGC                                                                                       >  SRR4302540.1038885/1‑94 (MQ=60)

         ATCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCG                                                                                      >  SRR4302540.8236740/1‑94 (MQ=60)

         ATCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCG                                                                                      >  SRR4302540.385905/1‑94 (MQ=60)

           CGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCC                                                                                    >  SRR4302540.9965533/1‑94 (MQ=60)

           CGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCC                                                                                    >  SRR4302540.1852249/1‑94 (MQ=60)

            GTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCG                                                                                   >  SRR4302540.8670205/1‑94 (MQ=60)

              AGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCC                                                                                 >  SRR4302540.8900454/1‑94 (MQ=60)

              AGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCC                                                                                 <  SRR4302540.5123728/94‑1 (MQ=60)

               GCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCA                                                                                >  SRR4302540.2232742/1‑94 (MQ=60)

                CTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAA                                                                               >  SRR4302540.6202625/1‑94 (MQ=60)

                CTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAA                                                                               <  SRR4302540.3277297/94‑1 (MQ=60)

                 TTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAAT                                                                              <  SRR4302540.4107555/94‑1 (MQ=60)

                 CTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAAT                                                                              <  SRR4302540.4925852/94‑1 (MQ=60)

                  TCCCAGCGACAGCGCCCCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATT                                                                             <  SRR4302540.7549171/94‑1 (MQ=60)

                   GCCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTC                                                                            >  SRR4302540.9271356/1‑94 (MQ=60)

                   ACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTC                                                                            >  SRR4302540.6285518/1‑94 (MQ=60)

                   ACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTC                                                                            <  SRR4302540.2095941/94‑1 (MQ=60)

                    CCAGCGACGGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCC                                                                           >  SRR4302540.6370889/1‑94 (MQ=60)

                    CCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCC                                                                           >  SRR4302540.428708/1‑94 (MQ=60)

                        CGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCT                                                                       <  SRR4302540.8193066/94‑1 (MQ=60)

                        CGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCT                                                                       <  SRR4302540.248643/94‑1 (MQ=60)

                         GACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTA                                                                      >  SRR4302540.3196617/1‑94 (MQ=60)

                         GACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTA                                                                      >  SRR4302540.2008668/1‑94 (MQ=60)

                          ACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTAT                                                                     >  SRR4302540.5860515/1‑94 (MQ=60)

                          ACAGCGCACCGCTCACGCCAACCGCGCAAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTAT                                                                     <  SRR4302540.8372774/94‑1 (MQ=60)

                           CAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATC                                                                    >  SRR4302540.6446256/1‑94 (MQ=60)

                            AGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCC                                                                   <  SRR4302540.8694872/94‑1 (MQ=60)

                            AGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCC                                                                   <  SRR4302540.7752698/94‑1 (MQ=60)

                             GCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCA                                                                  >  SRR4302540.8819816/1‑94 (MQ=60)

                             GCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCA                                                                  <  SRR4302540.4928101/94‑1 (MQ=60)

                              CGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAG                                                                 <  SRR4302540.5826967/94‑1 (MQ=60)

                              CGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAG                                                                 <  SRR4302540.4141521/94‑1 (MQ=60)

                                CACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCC                                                               >  SRR4302540.1722712/1‑94 (MQ=60)

                                 ACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCA                                                              >  SRR4302540.185469/1‑94 (MQ=60)

                                 ACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCA                                                              >  SRR4302540.736582/1‑94 (MQ=60)

                                 ACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCA                                                              >  SRR4302540.7938612/1‑94 (MQ=60)

                                 ACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCA                                                              >  SRR4302540.1120123/1‑94 (MQ=60)

                                  CCGCCCACCCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCAC                                                             <  SRR4302540.6050038/94‑1 (MQ=60)

                                   CGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACT                                                            >  SRR4302540.9327771/1‑94 (MQ=60)

                                    gccccccccgcCCGCGCCAGTCATTACAGTGACGCCCTCGGCTGCGGTATACAGCCTGCCAACCGCTCCGCCAATTCCAGCTATCCAGCCACTT                                                           <  SRR4302540.3320829/83‑1 (MQ=57)

                                    GCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTT                                                           >  SRR4302540.5241405/1‑94 (MQ=60)

                                     CTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTA                                                          <  SRR4302540.6605519/94‑1 (MQ=60)

                                     CTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTA                                                          >  SRR4302540.5324669/1‑94 (MQ=60)

                                      TCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAA                                                         >  SRR4302540.3828610/1‑94 (MQ=60)

                                        CCGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACG                                                       <  SRR4302540.7221035/94‑1 (MQ=60)

                                         CGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGC                                                      >  SRR4302540.2692465/1‑94 (MQ=60)

                                           CCAACCGCTCCGTTCAGTACAGGGAAGCCCGCGGCTTCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTT                                                    <  SRR4302540.3274690/94‑1 (MQ=60)

                                           CCAACCGCGCGAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTT                                                    <  SRR4302540.9281755/94‑1 (MQ=60)

                                           CCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTT                                                    >  SRR4302540.3230099/1‑94 (MQ=60)

                                           CCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTT                                                    >  SRR4302540.9663643/1‑94 (MQ=60)

                                           CCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTT                                                    >  SRR4302540.8614032/1‑94 (MQ=60)

                                           CCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTT                                                    >  SRR4302540.9851593/1‑94 (MQ=60)

                                            CAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTT                                                   >  SRR4302540.314204/1‑94 (MQ=60)

                                              ACCGCCCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTT                                                 <  SRR4302540.7998223/94‑1 (MQ=60)

                                               CCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTG                                                >  SRR4302540.7324999/1‑94 (MQ=60)

                                                CGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGA                                               >  SRR4302540.5433240/1‑94 (MQ=60)

                                                CGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGA                                               <  SRR4302540.2096334/94‑1 (MQ=60)

                                                 GCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAA                                              <  SRR4302540.2513670/94‑1 (MQ=60)

                                                  CGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAA                                             >  SRR4302540.3895747/1‑94 (MQ=60)

                                                  CGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAA                                             >  SRR4302540.3644445/1‑94 (MQ=60)

                                                    CCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAG                                           <  SRR4302540.1578966/94‑1 (MQ=60)

                                                     CAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGA                                          <  SRR4302540.6071905/94‑1 (MQ=60)

                                                     CAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTCTACGCTTTTTGAAAAGA                                          >  SRR4302540.9093738/1‑94 (MQ=60)

                                                     CAGTCAGGACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGA                                          <  SRR4302540.6071904/94‑1 (MQ=60)

                                                      AGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAA                                         <  SRR4302540.6680423/94‑1 (MQ=60)

                                                      AGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAA                                         >  SRR4302540.10162526/1‑94 (MQ=60)

                                                      AGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAA                                         >  SRR4302540.10162524/1‑94 (MQ=60)

                                                       GTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAA                                        <  SRR4302540.9738159/94‑1 (MQ=60)

                                                       GTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAA                                        <  SRR4302540.9738160/94‑1 (MQ=60)

                                                        TCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAGGAAAA                                       <  SRR4302540.9016204/94‑1 (MQ=60)

                                                         CAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAG                                      <  SRR4302540.557281/94‑1 (MQ=60)

                                                         CAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAG                                      <  SRR4302540.1043791/94‑1 (MQ=60)

                                                         CAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAG                                      >  SRR4302540.7144449/1‑94 (MQ=60)

                                                         CAGTACAGTGAAGCCCGCGGCGGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAG                                      <  SRR4302540.8006365/94‑1 (MQ=60)

                                                          AGTCCAGTGAAGCCCGCGGCTGCCGTATACGGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGA                                     <  SRR4302540.3292705/94‑1 (MQ=60)

                                                          AGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGA                                     <  SRR4302540.7207286/94‑1 (MQ=60)

                                                          AGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGA                                     <  SRR4302540.5479174/94‑1 (MQ=60)

                                                          AGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGA                                     <  SRR4302540.9043949/94‑1 (MQ=60)

                                                          AGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGA                                     >  SRR4302540.2570621/1‑94 (MQ=60)

                                                           GTACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAG                                    >  SRR4302540.4312199/1‑94 (MQ=60)

                                                            TACAGTGAAGCCGGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACTCTTTTTGAAAAGAAAAGAGA                                   <  SRR4302540.9859449/94‑1 (MQ=60)

                                                             ACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAA                                  <  SRR4302540.2635668/94‑1 (MQ=60)

                                                             ACAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAA                                  <  SRR4302540.624193/94‑1 (MQ=60)

                                                              CAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAA                                 <  SRR4302540.5234441/94‑1 (MQ=60)

                                                              CAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAA                                 <  SRR4302540.4219800/94‑1 (MQ=60)

                                                              CAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAA                                 <  SRR4302540.48593/94‑1 (MQ=60)

                                                              CAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAA                                 <  SRR4302540.4994529/94‑1 (MQ=60)

                                                              CAGTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAA                                 >  SRR4302540.3907794/1‑94 (MQ=60)

                                                                GTGAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAagcgctagatcggaagagcgggtcagcaggaatgc                               >  SRR4302540.643422/1‑59 (MQ=60)

                                                                  GAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCC                             >  SRR4302540.1600762/1‑94 (MQ=60)

                                                                  GAAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCC                             <  SRR4302540.9405922/94‑1 (MQ=60)

                                                                   AAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCA                            <  SRR4302540.6449240/94‑1 (MQ=60)

                                                                   AAGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCA                            <  SRR4302540.7759155/94‑1 (MQ=60)

                                                                   AAGCCCGCGGCTGCAGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCA                            >  SRR4302540.6792938/1‑94 (MQ=60)

                                                                    AGCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAG                           >  SRR4302540.8715434/1‑94 (MQ=60)

                                                                     GCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGA                          >  SRR4302540.4126521/1‑94 (MQ=60)

                                                                     GCCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGA                          <  SRR4302540.8176750/94‑1 (MQ=60)

                                                                     ACCCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGA                          <  SRR4302540.5231209/94‑1 (MQ=60)

                                                                       CCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAAC                        <  SRR4302540.564401/94‑1 (MQ=60)

                                                                       CCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAAC                        <  SRR4302540.1192321/94‑1 (MQ=60)

                                                                       CCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAAC                        <  SRR4302540.7416128/94‑1 (MQ=60)

                                                                       CCGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAAC                        <  SRR4302540.5712076/94‑1 (MQ=60)

                                                                        CGCGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACA                       <  SRR4302540.6572952/94‑1 (MQ=60)

                                                                          CTGCTGCCGTATACAGACTGCCAACCGCTCCGCTAATTCCGGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGC                     <  SRR4302540.3637537/94‑1 (MQ=60)

                                                                          CGGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGC                     >  SRR4302540.335248/1‑94 (MQ=60)

                                                                          CGCCTGCCGTATACAGACTGCCACCCGCGCCGCCAATTCCAGCTATCCAGCCACTTANCGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGC                     <  SRR4302540.4005339/94‑1 (MQ=60)

                                                                           GGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCG                    >  SRR4302540.945937/1‑94 (MQ=60)

                                                                           GGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCG                    <  SRR4302540.2486983/94‑1 (MQ=60)

                                                                           GGCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCG                    >  SRR4302540.5188211/1‑94 (MQ=60)

                                                                            GCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGG                   <  SRR4302540.10057223/94‑1 (MQ=60)

                                                                            GCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGG                   >  SRR4302540.1877397/1‑94 (MQ=60)

                                                                            GCTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGG                   <  SRR4302540.8870289/94‑1 (MQ=60)

                                                                             CTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGC                  >  SRR4302540.7707602/1‑94 (MQ=60)

                                                                             CTGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCCATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGC                  >  SRR4302540.7371802/1‑94 (MQ=60)

                                                                              TGCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCG                 >  SRR4302540.7303980/1‑94 (MQ=60)

                                                                               GCCGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGG                >  SRR4302540.9474230/1‑94 (MQ=60)

                                                                                 CGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCG              >  SRR4302540.10144532/1‑94 (MQ=60)

                                                                                 CGTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCG              >  SRR4302540.6682479/1‑94 (MQ=60)

                                                                                  GTATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGA             <  SRR4302540.235695/94‑1 (MQ=60)

                                                                                    ATACAGACTGCCAACCGCGCCGCGAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACA           >  SRR4302540.1864244/1‑94 (MQ=60)

                                                                                    ATACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGACAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACA           >  SRR4302540.4003909/1‑94 (MQ=60)

                                                                                     TACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACAA          <  SRR4302540.10137915/94‑1 (MQ=60)

                                                                                     TACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACAA          <  SRR4302540.306528/94‑1 (MQ=60)

                                                                                     TACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACAA          >  SRR4302540.4068746/1‑94 (MQ=60)

                                                                                      ACAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACAAA         >  SRR4302540.3220444/1‑94 (MQ=60)

                                                                                       CAGACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACAAAC        <  SRR4302540.2009037/94‑1 (MQ=60)

                                                                                        AGACTGCCAACCGCGCCGCCAATTCCAGCTACCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACAAACC       <  SRR4302540.2967672/94‑1 (MQ=60)

                                                                                         GACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACAAACCC      >  SRR4302540.9106231/1‑94 (MQ=60)

                                                                                         GACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACAAACCA      >  SRR4302540.5175744/1‑94 (MQ=60)

                                                                                          ACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACAAACCAC     <  SRR4302540.2550487/94‑1 (MQ=60)

                                                                                          ACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACAAACCAC     <  SRR4302540.1550025/94‑1 (MQ=60)

                                                                                          ACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACAAACCAC     <  SRR4302540.8086146/94‑1 (MQ=60)

                                                                                          ACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACAAACCAC     <  SRR4302540.8877030/94‑1 (MQ=60)

                                                                                          ACTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACAAACCAC     <  SRR4302540.2550486/94‑1 (MQ=60)

                                                                                           CTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACAAACCACG    >  SRR4302540.3920995/1‑94 (MQ=60)

                                                                                           CTGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACAAACCACG    >  SRR4302540.3926354/1‑94 (MQ=60)

                                                                                            TGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACAACCCACGC   >  SRR4302540.3719053/1‑94 (MQ=60)

                                                                                            TGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACAAACCACGC   >  SRR4302540.2055234/1‑94 (MQ=60)

                                                                                            TGCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACAAACCACGC   >  SRR4302540.2348649/1‑94 (MQ=60)

                                                                                             GCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACAAACCACGCC  >  SRR4302540.4907203/1‑94 (MQ=60)

                                                                                             GCCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACAAACCACGCC  <  SRR4302540.6808021/94‑1 (MQ=60)

GATTTGCACATCGTAGCTTACCAGCGACAGCGCACCGCTCACGCCAACCGCGCCAGTCAGTACAGTGAAGCCCGCGGCTGCCGTATACAGACTACCAACCGCGCCGCCAATTCCAGCTATCCAGCCACTTAACGCTTTTTGAAAAGAAAAGAGAAATGCCAGAACAGCGGCGGCGACAAACCACGCC  >  NC_000913/2847046‑2847232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`