Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,967,307 A→G D222G (GAC→GGC)  wzzE → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,967,3070AG99.8% 1271.1 / ‑6.1 395D222G (GAC→GGC) wzzEputative transport protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (186/207);  total (186/209)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 408 total aligned reads displayed.

ATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAAT  >  NC_000913/3967217‑3967396
                                                                                          |                                                                                         
atgatgAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGcc                                                                                         <  1:2268340/93‑1 (MQ=255)
atgatgAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGcc                                                                                         >  2:4106144/1‑93 (MQ=255)
atgatgAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGcc                                                                                         >  1:7008760/1‑93 (MQ=255)
  gatgaGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGccgc                                                                                       >  1:5875052/1‑93 (MQ=255)
  gatgaGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGccgc                                                                                       >  1:6340358/1‑93 (MQ=255)
  gatgaGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGccgc                                                                                       >  1:3233174/1‑93 (MQ=255)
  gatgaGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGGGGCGAAAGCCATCTACGGccgc                                                                                       >  2:6961035/1‑93 (MQ=255)
    tgaGCTGAAAGGCGCATGGGCGGCGCGTCCCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGccgccg                                                                                     <  1:2839040/93‑1 (MQ=255)
       gCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGAt                                                                                  >  1:3435853/1‑93 (MQ=255)
       gCTGAAAGCCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGTAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGAt                                                                                  <  1:1359959/93‑1 (MQ=255)
        cTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATg                                                                                 <  1:2011859/93‑1 (MQ=255)
         tGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGa                                                                                >  1:3756017/1‑93 (MQ=255)
          taaaGGCGCATGGGCGGCGCGTTCCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGaa                                                                               <  1:7596675/92‑1 (MQ=255)
          gAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGaa                                                                               <  1:2166231/93‑1 (MQ=255)
          gAAAGGCGAATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGaa                                                                               <  2:3077033/93‑1 (MQ=255)
             aGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAg                                                                            >  2:6525289/1‑93 (MQ=255)
               gcgcATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCa                                                                          <  1:9444945/93‑1 (MQ=255)
                  cATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTg                                                                       >  1:1944209/1‑93 (MQ=255)
                   aTGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGa                                                                      <  1:6610377/93‑1 (MQ=255)
                    tGGGCGGCGCGTCCCCTCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAg                                                                     <  2:8959557/93‑1 (MQ=255)
                      ggcggcGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCa                                                                   <  1:1125434/93‑1 (MQ=255)
                       gcggcgCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAg                                                                  <  2:2164309/93‑1 (MQ=255)
                        cggcgCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAgg                                                                 <  1:6129185/93‑1 (MQ=255)
                         ggcgCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGgc                                                                >  1:954043/1‑93 (MQ=255)
                          gcgcGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGgcg                                                               >  1:7462170/1‑93 (MQ=255)
                             cgTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTg                                                            >  1:4562649/1‑93 (MQ=255)
                             cgTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTg                                                            <  1:9749269/93‑1 (MQ=255)
                             cgTACCATCCAGATGAAAGCGCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTg                                                            <  1:8510202/93‑1 (MQ=255)
                               tACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCa                                                                   <  1:268357/84‑1 (MQ=255)
                               tACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGaa                                                          >  1:8259858/1‑93 (MQ=255)
                               tACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGaa                                                          >  1:1565809/1‑93 (MQ=255)
                                 ccATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGaaaa                                                        <  1:3021252/93‑1 (MQ=255)
                                 acATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGaaaa                                                        <  1:7642405/92‑1 (MQ=255)
                                  cATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAAt                                                       <  1:559464/93‑1 (MQ=255)
                                  cATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAAt                                                       >  1:3142006/1‑93 (MQ=255)
                                   aTCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAAtt                                                      <  2:1144688/93‑1 (MQ=255)
                                   aTCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAAtt                                                      <  2:8142089/93‑1 (MQ=255)
                                     ccAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGc                                                    <  2:9533278/93‑1 (MQ=255)
                                        gATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGa                                                 <  2:1021284/93‑1 (MQ=255)
                                        gATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGa                                                 >  2:9161853/1‑93 (MQ=255)
                                         aTGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGag                                                >  1:4183738/1‑93 (MQ=255)
                                          tGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGagc                                               >  2:390577/1‑93 (MQ=255)
                                          tGAAACCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGagc                                               <  2:3736603/93‑1 (MQ=255)
                                           gAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGagca                                              >  2:1925842/1‑93 (MQ=255)
                                                 tCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCataa                                        >  1:2480169/1‑93 (MQ=255)
                                                 tCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCataa                                        <  1:8097991/93‑1 (MQ=255)
                                                   aGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCataata                                      >  2:3195502/1‑93 (MQ=255)
                                                   aGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCataata                                      >  1:4295786/1‑93 (MQ=255)
                                                   aGGTGAAGCGTCAGGAAGAGGGGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCataata                                      >  2:4291555/1‑93 (MQ=255)
                                                     gTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATAtt                                    >  2:7240064/1‑93 (MQ=255)
                                                     gTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATAtt                                    <  2:6678658/93‑1 (MQ=255)
                                                       gAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTc                                  <  2:8554306/93‑1 (MQ=255)
                                                       gAAGCGGCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTc                                  <  2:8603255/93‑1 (MQ=255)
                                                        aaGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTcg                                 >  2:5126651/1‑93 (MQ=255)
                                                        aaGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTcg                                 <  2:704766/93‑1 (MQ=255)
                                                         aGCGTCAGGAAGAGGTGGTGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTcgc                                <  1:2906403/93‑1 (MQ=255)
                                                         aGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTcgc                                <  1:5048918/93‑1 (MQ=255)
                                                          gCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTcgcg                               >  1:3556408/1‑93 (MQ=255)
                                                            gTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGCTGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCa                             >  1:6524976/1‑93 (MQ=255)
                                                            gTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCa                             <  2:1684135/93‑1 (MQ=255)
                                                             tCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAg                            >  1:926148/1‑93 (MQ=255)
                                                             tCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAg                            >  2:7985247/1‑93 (MQ=255)
                                                             tCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAg                            >  1:8600566/1‑93 (MQ=255)
                                                              cAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGt                           <  1:6089258/93‑1 (MQ=255)
                                                              cAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGt                           <  2:2779014/93‑1 (MQ=255)
                                                               aGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTg                          >  2:1447591/1‑93 (MQ=255)
                                                                ggAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGc                         >  1:4498723/1‑93 (MQ=255)
                                                                ggAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGc                         <  2:2899969/93‑1 (MQ=255)
                                                                ggAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGc                         >  2:4453204/1‑93 (MQ=255)
                                                                 gAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCg                        <  2:2272813/93‑1 (MQ=255)
                                                                 gAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCg                        >  2:3521419/1‑93 (MQ=255)
                                                                 gAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCg                        >  2:3635719/1‑93 (MQ=255)
                                                                  aaGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGa                       <  2:9800410/93‑1 (MQ=255)
                                                                  aaGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAAAAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGAAGTGCGa                       >  1:7257655/1‑93 (MQ=255)
                                                                   agagGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGAc                      >  1:4796938/1‑93 (MQ=255)
                                                                    gagGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACa                     >  2:2565791/1‑93 (MQ=255)
                                                                      ggTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGa                   <  1:3654796/93‑1 (MQ=255)
                                                                      ggTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGa                   <  2:9332002/93‑1 (MQ=255)
                                                                      ggTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGa                   <  2:9004235/93‑1 (MQ=255)
                                                                       gTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGAt                  >  2:867540/1‑93 (MQ=255)
                                                                       gTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGAt                  >  2:6098533/1‑93 (MQ=255)
                                                                        tGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATg                 >  2:2665202/1‑93 (MQ=255)
                                                                          gCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTa               <  1:9963711/93‑1 (MQ=255)
                                                                           cGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTAc              <  1:7944954/93‑1 (MQ=255)
                                                                           cGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTAc              >  2:778304/1‑93 (MQ=255)
                                                                            gAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTAcc             <  1:10026374/93‑1 (MQ=255)
                                                                            gAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTAcc             <  1:5283085/93‑1 (MQ=255)
                                                                            gAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTAcc             >  2:5437836/1‑93 (MQ=255)
                                                                            gAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTAcc             <  1:687772/93‑1 (MQ=255)
                                                                            gAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTAcc             <  1:6435374/93‑1 (MQ=255)
                                                                             aaaGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCt            >  2:7623615/1‑93 (MQ=255)
                                                                             aaaGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCt            >  1:1671464/1‑93 (MQ=255)
                                                                             aaaGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCt            <  2:6212401/93‑1 (MQ=255)
                                                                                gCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGcc         >  2:4812389/1‑93 (MQ=255)
                                                                                   aTCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAg      >  2:8440095/1‑93 (MQ=255)
                                                                                   aTCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAg      >  1:3892114/1‑93 (MQ=255)
                                                                                    tCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAgg     <  2:7426350/93‑1 (MQ=255)
                                                                                    tCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAgg     >  1:9072964/1‑93 (MQ=255)
                                                                                    tCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAgg     >  2:5319048/1‑93 (MQ=255)
                                                                                       aCGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAAt  <  1:8739582/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAAT  >  NC_000913/3967217‑3967396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 233 total aligned reads displayed.
TGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACC  >  NC_000913/3967214‑3967400
                                                                                             |                                                                                             
TGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGG                                                                                               <  SRR4302540.1056766/94‑1 (MQ=60)
TGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGG                                                                                               >  SRR4302540.1254321/1‑94 (MQ=60)
 GAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGC                                                                                              <  SRR4302540.8740756/94‑1 (MQ=60)
  AATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCC                                                                                             >  SRR4302540.2609311/1‑94 (MQ=60)
  AATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCC                                                                                             <  SRR4302540.3409689/94‑1 (MQ=60)
  AATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCC                                                                                             >  SRR4302540.7086554/1‑94 (MQ=60)
  AAGGAGGAGCTGAAAGGCGCATGGGCGTCGCGTACCATCCAGATGAAAGCTCAGGGCAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCC                                                                                             <  SRR4302540.2796764/94‑1 (MQ=60)
   ATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCG                                                                                            <  SRR4302540.2292677/94‑1 (MQ=60)
    TGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGC                                                                                           >  SRR4302540.3268332/1‑94 (MQ=60)
    TGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGC                                                                                           >  SRR4302540.6410968/1‑94 (MQ=60)
    TGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGC                                                                                           >  SRR4302540.232710/1‑94 (MQ=60)
    TGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGC                                                                                           >  SRR4302540.5939995/1‑94 (MQ=60)
     GATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCC                                                                                          >  SRR4302540.5479629/1‑94 (MQ=60)
     GATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCC                                                                                          <  SRR4302540.8613359/94‑1 (MQ=60)
     GATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCC                                                                                          <  SRR4302540.6894264/94‑1 (MQ=60)
     GATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCC                                                                                          <  SRR4302540.9452679/94‑1 (MQ=60)
       TGAGCTGAAAGGCGCATGGGCGGCGCGTCCCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGG                                                                                        <  SRR4302540.2869750/94‑1 (MQ=60)
        GAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGA                                                                                       <  SRR4302540.6298198/94‑1 (MQ=60)
         AGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGAT                                                                                      <  SRR4302540.7071069/94‑1 (MQ=60)
         AGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGAT                                                                                      >  SRR4302540.3473202/1‑94 (MQ=60)
          GCTGAAAGCCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGTAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATG                                                                                     <  SRR4302540.1374307/94‑1 (MQ=60)
           CTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGA                                                                                    >  SRR4302540.3796992/1‑94 (MQ=60)
           CTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGA                                                                                    <  SRR4302540.5061791/94‑1 (MQ=60)
           CTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGA                                                                                    <  SRR4302540.2033616/94‑1 (MQ=60)
            TGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAA                                                                                   >  SRR4302540.9861099/1‑94 (MQ=60)
            CGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAA                                                                                   <  SRR4302540.7047964/94‑1 (MQ=60)
             TAAAGGCGCATGGGCGGCGCGTTCCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAAC                                                                                  <  SRR4302540.7682020/94‑1 (MQ=60)
             GAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAAC                                                                                  >  SRR4302540.3053709/1‑94 (MQ=60)
             GAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAAC                                                                                  <  SRR4302540.7682021/94‑1 (MQ=60)
             GAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAAC                                                                                  <  SRR4302540.2189564/94‑1 (MQ=60)
              AAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACA                                                                                 <  SRR4302540.9569853/94‑1 (MQ=60)
              AAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACA                                                                                 <  SRR4302540.4326431/94‑1 (MQ=60)
              AAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACA                                                                                 <  SRR4302540.6526975/94‑1 (MQ=60)
                  GCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCAT                                                                             <  SRR4302540.1951901/94‑1 (MQ=60)
                  GCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCAT                                                                             <  SRR4302540.9557073/94‑1 (MQ=60)
                   CGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATT                                                                            >  SRR4302540.4645337/1‑94 (MQ=60)
                   CGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATT                                                                            >  SRR4302540.8695493/1‑94 (MQ=60)
                   CGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATT                                                                            <  SRR4302540.3929604/94‑1 (MQ=60)
                   CGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATT                                                                            <  SRR4302540.386504/94‑1 (MQ=60)
                   CGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATT                                                                            <  SRR4302540.712551/94‑1 (MQ=60)
                    GCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTG                                                                           <  SRR4302540.7109004/94‑1 (MQ=60)
                    GCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTG                                                                           <  SRR4302540.3185506/94‑1 (MQ=60)
                    GCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTG                                                                           <  SRR4302540.1977717/94‑1 (MQ=60)
                    GCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTG                                                                           >  SRR4302540.1965374/1‑94 (MQ=60)
                     CATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGA                                                                          >  SRR4302540.1109342/1‑94 (MQ=60)
                     CATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGA                                                                          >  SRR4302540.2543932/1‑94 (MQ=60)
                     CATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGA                                                                          <  SRR4302540.4684076/94‑1 (MQ=60)
                     CATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGA                                                                          >  SRR4302540.8760886/1‑94 (MQ=60)
                      ATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAG                                                                         >  SRR4302540.6608409/1‑94 (MQ=60)
                      ATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAG                                                                         <  SRR4302540.6683568/94‑1 (MQ=60)
                      ATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAG                                                                         >  SRR4302540.3081982/1‑94 (MQ=60)
                      ATGGGAGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAG                                                                         <  SRR4302540.6683571/94‑1 (MQ=60)
                         tagcgcttGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAG                                                                      <  SRR4302540.271003/86‑1 (MQ=60)
                         GGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAG                                                                      <  SRR4302540.2880994/94‑1 (MQ=60)
                         GGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAG                                                                      <  SRR4302540.1137142/94‑1 (MQ=60)
                         GGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAG                                                                      <  SRR4302540.7180758/94‑1 (MQ=60)
                          GCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGG                                                                     <  SRR4302540.2013111/94‑1 (MQ=60)
                           CGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGC                                                                    <  SRR4302540.6197454/94‑1 (MQ=60)
                           CGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGC                                                                    <  SRR4302540.3666537/94‑1 (MQ=60)
                           CGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGC                                                                    >  SRR4302540.963941/1‑94 (MQ=60)
                           CGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGC                                                                    <  SRR4302540.6197453/94‑1 (MQ=60)
                            GGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCG                                                                   >  SRR4302540.7540704/1‑94 (MQ=60)
                              CGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCT                                                                 >  SRR4302540.2129204/1‑94 (MQ=60)
                              CGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCT                                                                 <  SRR4302540.901935/94‑1 (MQ=60)
                               GCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCGTTGAGCAGGCGCTG                                                                <  SRR4302540.6614574/94‑1 (MQ=60)
                               GCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTG                                                                >  SRR4302540.4611936/1‑94 (MQ=60)
                               GCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTG                                                                <  SRR4302540.4260180/94‑1 (MQ=60)
                                CGTCCCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGAGGAACAGCATTGAGCAGGCGCTGA                                                               <  SRR4302540.2873657/94‑1 (MQ=60)
                                CGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGA                                                               <  SRR4302540.9863675/94‑1 (MQ=60)
                                CGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGA                                                               <  SRR4302540.8140773/94‑1 (MQ=60)
                                CGTACCATCCAGATGAAAGCGCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGA                                                               <  SRR4302540.8609103/94‑1 (MQ=60)
                                 GTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAA                                                              >  SRR4302540.8355842/1‑94 (MQ=60)
                                 GTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAA                                                              >  SRR4302540.1582991/1‑94 (MQ=60)
                                  TACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAA                                                             >  SRR4302540.9604065/1‑94 (MQ=60)
                                  TACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAA                                                             >  SRR4302540.3368317/1‑94 (MQ=60)
                                   ACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAA                                                            <  SRR4302540.4388658/94‑1 (MQ=60)
                                    CCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAAT                                                           >  SRR4302540.4056871/1‑94 (MQ=60)
                                    CCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAAT                                                           >  SRR4302540.3176258/1‑94 (MQ=60)
                                    CCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAAT                                                           >  SRR4302540.2815490/1‑94 (MQ=60)
                                    CCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAAT                                                           <  SRR4302540.3054458/94‑1 (MQ=60)
                                    ACATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAAT                                                           <  SRR4302540.7728419/94‑1 (MQ=60)
                                     CATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATT                                                          >  SRR4302540.10051642/1‑94 (MQ=60)
                                     CATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATT                                                          <  SRR4302540.565548/94‑1 (MQ=60)
                                     CATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATT                                                          >  SRR4302540.6626709/1‑94 (MQ=60)
                                     CATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGATTTGAGCAGGCGGTGAAACTT                                                          >  SRR4302540.3190879/1‑94 (MQ=60)
                                       TCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGGTGAACAGCATTGAGCAGGCGCTGAAAATTGC                                                        >  SRR4302540.2128212/1‑94 (MQ=60)
                                       TCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCAGCCGGATGAACAGCATTGCGCAGGCGCTGAAAATTGC                                                        >  SRR4302540.6194339/1‑94 (MQ=60)
                                        CCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGGAAGCCATCTACGGCCGCCGGATGAACAGCATTGCGCAGGCGCTGAAAATTGCT                                                       >  SRR4302540.7670709/1‑94 (MQ=60)
                                        CCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCT                                                       >  SRR4302540.1315807/1‑94 (MQ=60)
                                        CCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCT                                                       >  SRR4302540.6184208/1‑94 (MQ=60)
                                         CAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACCGCATTGAGCAGGCGCTGAAAATTGCTG                                                      >  SRR4302540.1538260/1‑94 (MQ=60)
                                         CAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTG                                                      >  SRR4302540.3580422/1‑94 (MQ=60)
                                          AGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGG                                                     >  SRR4302540.1546944/1‑94 (MQ=60)
                                           GATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAG                                                    >  SRR4302540.4229199/1‑94 (MQ=60)
                                             TGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCA                                                  <  SRR4302540.6495178/94‑1 (MQ=60)
                                              GAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAG                                                 <  SRR4302540.8766343/94‑1 (MQ=60)
                                               AAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGC                                                >  SRR4302540.1655790/1‑94 (MQ=60)
                                               AAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGC                                                <  SRR4302540.5681470/94‑1 (MQ=60)
                                               AAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGC                                                >  SRR4302540.8194739/1‑94 (MQ=60)
                                                 AGCTCAGGTTAAGCGGCATGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCAT                                              <  SRR4302540.7908985/94‑1 (MQ=60)
                                                 AGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCAA                                              >  SRR4302540.3642057/1‑94 (MQ=60)
                                                  GCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATA                                             >  SRR4302540.2899684/1‑94 (MQ=60)
                                                  GCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATA                                             >  SRR4302540.7515537/1‑94 (MQ=60)
                                                  GCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATA                                             <  SRR4302540.4097643/94‑1 (MQ=60)
                                                   CTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAA                                            <  SRR4302540.9260227/94‑1 (MQ=60)
                                                   CTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAA                                            >  SRR4302540.3052543/1‑94 (MQ=60)
                                                   CTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAA                                            >  SRR4302540.5441054/1‑94 (MQ=60)
                                                   CTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAA                                            >  SRR4302540.4374053/1‑94 (MQ=60)
                                                    TCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAAT                                           <  SRR4302540.6478170/94‑1 (MQ=60)
                                                    TCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAAT                                           <  SRR4302540.9936242/94‑1 (MQ=60)
                                                    TCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAAT                                           >  SRR4302540.7685819/1‑94 (MQ=60)
                                                    TCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAAT                                           <  SRR4302540.8191985/94‑1 (MQ=60)
                                                     CAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATA                                          >  SRR4302540.1631412/1‑94 (MQ=60)
                                                     CAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATA                                          >  SRR4302540.4342529/1‑94 (MQ=60)
                                                     CAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATA                                          >  SRR4302540.3713841/1‑94 (MQ=60)
                                                     CAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATA                                          >  SRR4302540.8574627/1‑94 (MQ=60)
                                                      AGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATAT                                         >  SRR4302540.2225287/1‑94 (MQ=60)
                                                       GGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATT                                        >  SRR4302540.2153220/1‑94 (MQ=60)
                                                        GTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAATAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTT                                       <  SRR4302540.3789091/94‑1 (MQ=60)
                                                        GTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTT                                       >  SRR4302540.700013/1‑94 (MQ=60)
                                                        GTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTT                                       <  SRR4302540.1069553/94‑1 (MQ=60)
                                                        GTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTT                                       <  SRR4302540.3030327/94‑1 (MQ=60)
                                                         TGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTC                                      <  SRR4302540.6856911/94‑1 (MQ=60)
                                                         TGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTC                                      <  SRR4302540.4572447/94‑1 (MQ=60)
                                                            AGCGTCAGGAAGAGGTGGTGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCG                                   <  SRR4302540.2938138/94‑1 (MQ=60)
                                                            AGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATTTTTCGCG                                   <  SRR4302540.7396767/94‑1 (MQ=60)
                                                            AGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCG                                   >  SRR4302540.9783744/1‑94 (MQ=60)
                                                            AGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCG                                   <  SRR4302540.5103515/94‑1 (MQ=60)
                                                             GCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGC                                  <  SRR4302540.5087765/94‑1 (MQ=60)
                                                             GCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGC                                  <  SRR4302540.3663517/94‑1 (MQ=60)
                                                              CGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGCTGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCA                                 >  SRR4302540.6597444/1‑94 (MQ=60)
                                                               GTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAG                                >  SRR4302540.5202816/1‑94 (MQ=60)
                                                               GTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAG                                >  SRR4302540.935901/1‑94 (MQ=60)
                                                               GTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAG                                <  SRR4302540.3855627/94‑1 (MQ=60)
                                                               GTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAG                                <  SRR4302540.1960988/94‑1 (MQ=60)
                                                               GTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAG                                <  SRR4302540.4633837/94‑1 (MQ=60)
                                                               GTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAG                                <  SRR4302540.8674153/94‑1 (MQ=60)
                                                                TCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGG                               >  SRR4302540.2451063/1‑94 (MQ=60)
                                                                 CAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTG                              <  SRR4302540.8908637/94‑1 (MQ=60)
                                                                 CAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTG                              <  SRR4302540.6157024/94‑1 (MQ=60)
                                                                  AGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGC                             <  SRR4302540.7662693/94‑1 (MQ=60)
                                                                  AGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGC                             >  SRR4302540.4547493/1‑94 (MQ=60)
                                                                  AGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGC                             <  SRR4302540.879150/94‑1 (MQ=60)
                                                                   GGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCG                            >  SRR4302540.9459552/1‑94 (MQ=60)
                                                                   GGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCG                            <  SRR4302540.4887225/94‑1 (MQ=60)
                                                                    GAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGA                           <  SRR4302540.3323145/94‑1 (MQ=60)
                                                                    GAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGA                           >  SRR4302540.9285183/1‑94 (MQ=60)
                                                                    GAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAAAAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGAAGTGCGA                           >  SRR4302540.7338636/1‑94 (MQ=60)
                                                                     AAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGAC                          >  SRR4302540.2216957/1‑94 (MQ=60)
                                                                     AAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGAC                          >  SRR4302540.4848272/1‑94 (MQ=60)
                                                                      AGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACA                         <  SRR4302540.5935884/94‑1 (MQ=60)
                                                                      AGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACA                         <  SRR4302540.6001481/94‑1 (MQ=60)
                                                                      AGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACA                         <  SRR4302540.10091237/94‑1 (MQ=60)
                                                                      AGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATCTCGCGCAGTGCGACA                         >  SRR4302540.765335/1‑94 (MQ=60)
                                                                       GAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAG                        <  SRR4302540.9551576/94‑1 (MQ=60)
                                                                       GAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAG                        <  SRR4302540.8238492/94‑1 (MQ=60)
                                                                         GGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGAT                      <  SRR4302540.3694826/94‑1 (MQ=60)
                                                                         GGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGAT                      <  SRR4302540.3569552/94‑1 (MQ=60)
                                                                         GGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGAT                      >  SRR4302540.3065127/1‑94 (MQ=60)
                                                                         GGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGAT                      <  SRR4302540.2835066/94‑1 (MQ=60)
                                                                         GGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGAT                      <  SRR4302540.161026/94‑1 (MQ=60)
                                                                          GTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATG                     <  SRR4302540.4466408/94‑1 (MQ=60)
                                                                          GTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATG                     >  SRR4302540.8055317/1‑94 (MQ=60)
                                                                           caatgctgttCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGT                    <  SRR4302540.6491139/84‑1 (MQ=60)
                                                                           TGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGT                    <  SRR4302540.9733907/94‑1 (MQ=60)
                                                                            GGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTA                   >  SRR4302540.3179855/1‑94 (MQ=60)
                                                                            GGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTA                   <  SRR4302540.6497963/94‑1 (MQ=60)
                                                                            GGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTA                   <  SRR4302540.6537654/94‑1 (MQ=60)
                                                                             GCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTAC                  >  SRR4302540.2631505/1‑94 (MQ=60)
                                                                             GCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTAC                  <  SRR4302540.10079962/94‑1 (MQ=60)
                                                                             GCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTAC                  <  SRR4302540.6550950/94‑1 (MQ=60)
                                                                              CGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACC                 <  SRR4302540.8037558/94‑1 (MQ=60)
                                                                              CGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACC                 >  SRR4302540.7860123/1‑94 (MQ=60)
                                                                               GAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCT                <  SRR4302540.8603981/94‑1 (MQ=60)
                                                                               GAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCT                <  SRR4302540.10143036/94‑1 (MQ=60)
                                                                               GAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCT                <  SRR4302540.5340809/94‑1 (MQ=60)
                                                                               GAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCT                <  SRR4302540.5136224/94‑1 (MQ=60)
                                                                               GAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCT                >  SRR4302540.831195/1‑94 (MQ=60)
                                                                               GAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCT                <  SRR4302540.779950/94‑1 (MQ=60)
                                                                               GAAAGCAATCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTCCCT                <  SRR4302540.1370909/94‑1 (MQ=60)
                                                                                   GCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCG            <  SRR4302540.6194058/94‑1 (MQ=60)
                                                                                     CATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAG          >  SRR4302540.3934366/1‑94 (MQ=60)
                                                                                      ATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCANTGCGACAGATGTACCTGCCGAGG         >  SRR4302540.5454629/1‑94 (MQ=60)
                                                                                      ATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCTAGG         >  SRR4302540.4584637/1‑94 (MQ=60)
                                                                                      ATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGG         <  SRR4302540.35211/94‑1 (MQ=60)
                                                                                      ATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGG         >  SRR4302540.6093106/1‑94 (MQ=60)
                                                                                      ATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGG         >  SRR4302540.1841432/1‑94 (MQ=60)
                                                                                      ATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGG         >  SRR4302540.6901823/1‑94 (MQ=60)
                                                                                      ATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGG         >  SRR4302540.9229860/1‑94 (MQ=60)
                                                                                      ATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGG         >  SRR4302540.9181921/1‑94 (MQ=60)
                                                                                       TCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGA        >  SRR4302540.4025270/1‑94 (MQ=60)
                                                                                        CTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAA       >  SRR4302540.2382428/1‑94 (MQ=60)
                                                                                        CTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAA       >  SRR4302540.1247499/1‑94 (MQ=60)
                                                                                          ACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATT     <  SRR4302540.8841038/94‑1 (MQ=60)
                                                                                            GGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTAC   <  SRR4302540.5744632/94‑1 (MQ=60)
                                                                                            GGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTAC   >  SRR4302540.7318270/1‑94 (MQ=60)
                                                                                            GGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTAC   <  SRR4302540.3935035/94‑1 (MQ=60)
                                                                                             GCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACC  >  SRR4302540.3654029/1‑94 (MQ=60)
                                                                                             GCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACC  <  SRR4302540.1697875/94‑1 (MQ=60)
                                                                                             GCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACC  <  SRR4302540.6180429/94‑1 (MQ=60)
                                                                                             |                                                                                             
TGAATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACC  >  NC_000913/3967214‑3967400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑