Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,011,430 A→G H263R (CAT→CGT)  metE → tetrahydropteroyltriglutamate methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,011,4300AG99.0% 1210.4 / ‑2.1 380H263R (CAT→CGT) metEtetrahydropteroyltriglutamate methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (1/3);  new base G (192/184);  total (193/187)
Fisher's exact test for biased strand distribution p-value = 3.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 399 total aligned reads displayed.

AGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCATGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATG  >  NC_000913/4011340‑4011519
                                                                                          |                                                                                         
aGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTg                                                                                         >  1:7193149/1‑93 (MQ=255)
 gggACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCgtgt                                                                                        <  2:9833923/93‑1 (MQ=255)
 gggACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCgtgt                                                                                        >  2:7375911/1‑93 (MQ=255)
 gggACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCgtgt                                                                                        >  2:5020742/1‑93 (MQ=255)
 gggACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCgtgt                                                                                        >  1:2406744/1‑93 (MQ=255)
  ggACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGtt                                                                                       <  1:2891486/93‑1 (MQ=255)
     cAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGAc                                                                                    <  2:3164244/93‑1 (MQ=255)
     cAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGAc                                                                                    >  1:8788763/1‑93 (MQ=255)
     cAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGAc                                                                                    <  1:3332971/93‑1 (MQ=255)
      aGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGAcc                                                                                   >  1:5707377/1‑93 (MQ=255)
       ggTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCt                                                                                  <  2:4732581/93‑1 (MQ=255)
       ggTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCt                                                                                  >  2:4115791/1‑93 (MQ=255)
       ggTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCt                                                                                  <  2:3891835/93‑1 (MQ=255)
        gTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTc                                                                                 <  1:9171575/93‑1 (MQ=255)
        gTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTc                                                                                 <  1:2859407/93‑1 (MQ=255)
         tGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCg                                                                                >  1:1628822/1‑93 (MQ=255)
         tGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCg                                                                                >  2:7034248/1‑93 (MQ=255)
          gAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGt                                                                               >  1:208659/1‑93 (MQ=255)
           aaaCTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGACTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTa                                                                              <  2:9195245/93‑1 (MQ=255)
            aaCTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTAc                                                                             >  2:3095597/1‑93 (MQ=255)
            aaCTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTAc                                                                             >  2:967129/1‑93 (MQ=255)
              ctggtgctgACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACAt                                                                           <  1:6396194/93‑1 (MQ=255)
              ctgctgctgACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACAt                                                                           <  2:7186370/93‑1 (MQ=255)
              ctgctgctgACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACAt                                                                           <  1:4191101/93‑1 (MQ=255)
                 ctgctgACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGt                                                                        >  1:1533491/1‑93 (MQ=255)
                  tgctgACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTa                                                                       <  1:6201191/93‑1 (MQ=255)
                   gctgAACAATTATTTTGAAGGCGTAACGCCAAATCACGACGCGATTACTGTGCTTCCTGTTCAGGGTCTGCGTGTTGACTTCGTACATGGTaa                                                                      <  2:6305603/93‑1 (MQ=255)
                     tgACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAg                                                                    >  1:7107102/1‑93 (MQ=255)
                     tgACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAg                                                                    >  2:7663593/1‑93 (MQ=255)
                     tgACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAg                                                                    >  2:897494/1‑93 (MQ=255)
                      gACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGa                                                                   >  2:10044986/1‑93 (MQ=255)
                      gACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGa                                                                   >  2:357280/1‑93 (MQ=255)
                      gACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGa                                                                   >  1:3770367/1‑93 (MQ=255)
                        ccaccTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATg                                                                 >  1:4934119/1‑93 (MQ=255)
                        ccaccTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATg                                                                 >  1:2226605/1‑93 (MQ=255)
                        ccaccTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATg                                                                 <  2:1234337/93‑1 (MQ=255)
                           ccTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACg                                                              <  2:6637272/93‑1 (MQ=255)
                            cTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGt                                                             >  1:6121992/1‑93 (MQ=255)
                            cTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGt                                                             <  1:3969542/93‑1 (MQ=255)
                            cTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGt                                                             >  1:7562440/1‑93 (MQ=255)
                             tATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGtt                                                            <  1:2196894/93‑1 (MQ=255)
                              aTTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTg                                                           >  1:3499889/1‑93 (MQ=255)
                               ttttGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGc                                                          <  2:4683961/93‑1 (MQ=255)
                               ttttGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGc                                                          <  1:5375475/93‑1 (MQ=255)
                                 ttGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTg                                                        <  2:8816628/93‑1 (MQ=255)
                                  tGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGa                                                       >  2:3517767/1‑93 (MQ=255)
                                   gAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGaa                                                      <  2:2876984/93‑1 (MQ=255)
                                    aaGGCGTAACTCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTTACCTCGTACATGGTAAAGATGACGTTGCTGAAc                                                     <  1:1364806/93‑1 (MQ=255)
                                     aGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACt                                                    <  1:4141095/93‑1 (MQ=255)
                                      ggCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTg                                                   >  1:6988947/1‑93 (MQ=255)
                                        cGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGca                                                 <  1:251153/93‑1 (MQ=255)
                                          tAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGcaca                                               >  1:9401634/1‑93 (MQ=255)
                                           aaCGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACaa                                              <  2:9552962/93‑1 (MQ=255)
                                           aaCGCCAAATCTCGACACGATCACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACaa                                              <  2:331284/93‑1 (MQ=255)
                                            aCGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAg                                             <  1:47475/93‑1 (MQ=255)
                                             cGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAgc                                            <  1:129432/93‑1 (MQ=255)
                                             cGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAgc                                            <  1:9428430/93‑1 (MQ=255)
                                             cGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAgc                                            <  2:652580/93‑1 (MQ=255)
                                             cGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAgc                                            >  2:8465041/1‑93 (MQ=255)
                                              gCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAgcg                                           >  1:9711058/1‑93 (MQ=255)
                                               ccAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAgcgc                                          <  2:5569056/93‑1 (MQ=255)
                                               ccAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAgcgc                                          <  1:6492769/93‑1 (MQ=255)
                                                cAAATCTCGACACGAGTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCgcc                                         <  1:2755318/93‑1 (MQ=255)
                                                 aaaTCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCgcct                                        >  2:1820664/1‑93 (MQ=255)
                                                 aaaTCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCgcct                                        >  1:121102/1‑93 (MQ=255)
                                                 aaaTCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCgcct                                        >  1:7498590/1‑93 (MQ=255)
                                                 aaaTCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCgcct                                        <  1:7913707/93‑1 (MQ=255)
                                                   aTCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCgcctgc                                      <  2:6109333/93‑1 (MQ=255)
                                                    tctcGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCgcctgcc                                     >  2:1998161/1‑93 (MQ=255)
                                                     ctcGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCgcctgcct                                    <  2:7269294/93‑1 (MQ=255)
                                                      tcGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCtt                                   <  1:2584317/93‑1 (MQ=255)
                                                       cGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTc                                  <  1:5220233/93‑1 (MQ=255)
                                                       cGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCATGTTGAACTCGTACATGGTAAAGATGAAGTTTCTGAACTCCCCAAGAGCCCGCAttt                                  >  1:8112533/1‑92 (MQ=255)
                                                          cacGATTACTGCGCTGCCTGTTCCGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGa                               <  2:2307497/93‑1 (MQ=255)
                                                          cacGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGa                               <  1:3675246/93‑1 (MQ=255)
                                                            cGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACt                             >  2:597365/1‑93 (MQ=255)
                                                              aTTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTgg                           <  1:6834429/93‑1 (MQ=255)
                                                               ttACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGt                          >  2:1056295/1‑93 (MQ=255)
                                                                tACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGtt                         >  2:4350441/1‑93 (MQ=255)
                                                                tACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGtt                         <  2:7846309/93‑1 (MQ=255)
                                                                tACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGtt                         >  2:6755891/1‑93 (MQ=255)
                                                                tACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGtt                         >  2:4515858/1‑93 (MQ=255)
                                                                    gcgcTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTg                     >  1:8383010/1‑93 (MQ=255)
                                                                     cgcTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGt                    <  1:5506137/93‑1 (MQ=255)
                                                                      gcTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTc                   <  2:4606303/93‑1 (MQ=255)
                                                                       cTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCt                  <  1:171100/93‑1 (MQ=255)
                                                                        tGCCTGTTCTGGGTCTGCGTGTTGCCCTCGTACATGGTAAAGCTGCCGTTGCTGACCTGCACCAGCGCCTGCCTTCTGACTGGTTGCTGTCTg                 <  1:7693154/93‑1 (MQ=255)
                                                                        tGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTg                 >  1:911775/1‑93 (MQ=255)
                                                                         gCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGc                <  2:8644628/93‑1 (MQ=255)
                                                                            tGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCggg             >  2:5242487/1‑93 (MQ=255)
                                                                               tCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCt          >  1:1032341/1‑93 (MQ=255)
                                                                               tCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCt          <  1:4453056/93‑1 (MQ=255)
                                                                               tCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCt          <  1:2273160/93‑1 (MQ=255)
                                                                               tCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCt          >  2:7993269/1‑93 (MQ=255)
                                                                                 aGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGa        >  2:8351318/1‑93 (MQ=255)
                                                                                 aGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGa        <  2:5452167/93‑1 (MQ=255)
                                                                                  gggTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGAt       <  2:6057314/93‑1 (MQ=255)
                                                                                    gTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCa     <  1:7327080/93‑1 (MQ=255)
                                                                                    gTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCa     >  1:3380968/1‑93 (MQ=255)
                                                                                     tCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCaa    >  1:8664799/1‑93 (MQ=255)
                                                                                          |                                                                                         
AGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCATGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATG  >  NC_000913/4011340‑4011519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 227 total aligned reads displayed.
CCAGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCATGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGGTCG  >  NC_000913/4011338‑4011523
                                                                                            |                                                                                             
 CAGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTG                                                                                             <  SRR4302540.9894345/94‑1 (MQ=60)
 CAGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTG                                                                                             >  SRR4302540.7273332/1‑94 (MQ=60)
  AGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGT                                                                                            >  SRR4302540.2432693/1‑94 (MQ=60)
  AGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGT                                                                                            >  SRR4302540.7853716/1‑94 (MQ=60)
   GGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTT                                                                                           >  SRR4302540.5128056/1‑94 (MQ=60)
    GGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTG                                                                                          <  SRR4302540.5408628/94‑1 (MQ=60)
    GGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTG                                                                                          >  SRR4302540.446512/1‑94 (MQ=60)
    GGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTG                                                                                          >  SRR4302540.4389227/1‑94 (MQ=60)
    GGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTG                                                                                          <  SRR4302540.2922998/94‑1 (MQ=60)
     GACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGA                                                                                         <  SRR4302540.5258081/94‑1 (MQ=60)
      ACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGAC                                                                                        <  SRR4302540.2531241/94‑1 (MQ=60)
      ACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGAC                                                                                        <  SRR4302540.2899415/94‑1 (MQ=60)
      ACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGAC                                                                                        <  SRR4302540.10065566/94‑1 (MQ=60)
       CAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACC                                                                                       <  SRR4302540.3487867/94‑1 (MQ=60)
       CAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACC                                                                                       >  SRR4302540.7700728/1‑94 (MQ=60)
       CAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACC                                                                                       <  SRR4302540.3369569/94‑1 (MQ=60)
       CAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACC                                                                                       >  SRR4302540.5770210/1‑94 (MQ=60)
        AGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCT                                                                                      >  SRR4302540.3632064/1‑94 (MQ=60)
        AGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCT                                                                                      >  SRR4302540.5775882/1‑94 (MQ=60)
         GGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTC                                                                                     <  SRR4302540.3591573/94‑1 (MQ=60)
          GTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCG                                                                                    >  SRR4302540.1646655/1‑94 (MQ=60)
          GTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCG                                                                                    <  SRR4302540.4954236/94‑1 (MQ=60)
          GTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCG                                                                                    <  SRR4302540.2890399/94‑1 (MQ=60)
          GTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCG                                                                                    >  SRR4302540.2876470/1‑94 (MQ=60)
          GTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCG                                                                                    <  SRR4302540.9281595/94‑1 (MQ=60)
          GTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCG                                                                                    <  SRR4302540.8980434/94‑1 (MQ=60)
          GTGAAACGGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCG                                                                                    <  SRR4302540.1588474/94‑1 (MQ=60)
           TGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGT                                                                                   <  SRR4302540.7649427/94‑1 (MQ=60)
           TGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGT                                                                                   >  SRR4302540.8008694/1‑94 (MQ=60)
           TGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGT                                                                                   >  SRR4302540.210708/1‑94 (MQ=60)
            GAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTA                                                                                  >  SRR4302540.7547122/1‑94 (MQ=60)
             AAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTAC                                                                                 >  SRR4302540.226973/1‑94 (MQ=60)
             AAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTAC                                                                                 >  SRR4302540.2914037/1‑94 (MQ=60)
              AACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACA                                                                                >  SRR4302540.7331199/1‑94 (MQ=60)
              AACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACA                                                                                <  SRR4302540.7085281/94‑1 (MQ=60)
              AACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACA                                                                                >  SRR4302540.1230639/1‑94 (MQ=60)
               ACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACAT                                                                               <  SRR4302540.2729728/94‑1 (MQ=60)
                CTGGTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATG                                                                              <  SRR4302540.6467393/94‑1 (MQ=60)
                CTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATG                                                                              <  SRR4302540.6467394/94‑1 (MQ=60)
                CTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATG                                                                              <  SRR4302540.4236683/94‑1 (MQ=60)
                CTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATG                                                                              >  SRR4302540.6562074/1‑94 (MQ=60)
                CTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATG                                                                              <  SRR4302540.2861421/94‑1 (MQ=60)
                 TGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGG                                                                             >  SRR4302540.1194512/1‑94 (MQ=60)
                  GCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGT                                                                            >  SRR4302540.3415849/1‑94 (MQ=60)
                  GCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGT                                                                            >  SRR4302540.5764296/1‑94 (MQ=60)
                  GCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGA                                                                            >  SRR4302540.2695771/1‑94 (MQ=60)
                   CTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTA                                                                           >  SRR4302540.9749734/1‑94 (MQ=60)
                   CTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTAGTACATGGTA                                                                           <  SRR4302540.9534592/94‑1 (MQ=60)
                    TGCTGACCACCTATTTTGAAGGCGTACCGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAA                                                                          <  SRR4302540.2511515/94‑1 (MQ=60)
                    TGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAA                                                                          >  SRR4302540.4710810/1‑94 (MQ=60)
                    TGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAA                                                                          <  SRR4302540.6270222/94‑1 (MQ=60)
                     GCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAA                                                                         <  SRR4302540.7215186/94‑1 (MQ=60)
                      CTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAG                                                                        <  SRR4302540.315211/94‑1 (MQ=60)
                      CTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAG                                                                        >  SRR4302540.3874386/1‑94 (MQ=60)
                      CTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAG                                                                        >  SRR4302540.7186187/1‑94 (MQ=60)
                      CTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAG                                                                        >  SRR4302540.5712722/1‑94 (MQ=60)
                      CTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAG                                                                        >  SRR4302540.2601422/1‑94 (MQ=60)
                      CTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGCACATGGTAAAG                                                                        >  SRR4302540.2783946/1‑94 (MQ=60)
                       TGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGA                                                                       >  SRR4302540.3811499/1‑94 (MQ=60)
                        GACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGAT                                                                      <  SRR4302540.1701962/94‑1 (MQ=60)
                        GACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGAT                                                                      <  SRR4302540.2156342/94‑1 (MQ=60)
                        GACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGAT                                                                      <  SRR4302540.4196949/94‑1 (MQ=60)
                         ACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATG                                                                     <  SRR4302540.6987848/94‑1 (MQ=60)
                         ACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATG                                                                     <  SRR4302540.7507459/94‑1 (MQ=60)
                         ACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATG                                                                     >  SRR4302540.2250595/1‑94 (MQ=60)
                         ACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATG                                                                     <  SRR4302540.8880404/94‑1 (MQ=60)
                         ACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATG                                                                     >  SRR4302540.4987333/1‑94 (MQ=60)
                          CCGCCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCATGTTGACCTCGTACATGGTAAAGATGA                                                                    <  SRR4302540.8821623/94‑1 (MQ=60)
                          CCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGC                                                                    >  SRR4302540.1458688/1‑94 (MQ=60)
                          CCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGA                                                                    >  SRR4302540.9161547/1‑94 (MQ=60)
                          CCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGA                                                                    >  SRR4302540.1458689/1‑94 (MQ=60)
                           CACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGAC                                                                   >  SRR4302540.6238276/1‑94 (MQ=60)
                           CACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGAC                                                                   <  SRR4302540.2006967/94‑1 (MQ=60)
                           CACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGAC                                                                   >  SRR4302540.6901760/1‑94 (MQ=60)
                            ACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACG                                                                  >  SRR4302540.4833151/1‑94 (MQ=60)
                            ACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACG                                                                  >  SRR4302540.2247491/1‑94 (MQ=60)
                            ACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACG                                                                  <  SRR4302540.5804884/94‑1 (MQ=60)
                             CCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGT                                                                 >  SRR4302540.3668599/1‑94 (MQ=60)
                             CCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGT                                                                 >  SRR4302540.6190176/1‑94 (MQ=60)
                               TATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTG                                                               <  SRR4302540.2220619/94‑1 (MQ=60)
                               TATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTG                                                               <  SRR4302540.2220620/94‑1 (MQ=60)
                               TATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTG                                                               >  SRR4302540.3538005/1‑94 (MQ=60)
                                ATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGC                                                              >  SRR4302540.4001165/1‑94 (MQ=60)
                                ATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGC                                                              >  SRR4302540.9515384/1‑94 (MQ=60)
                                ATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGC                                                              >  SRR4302540.2542627/1‑94 (MQ=60)
                                 TTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCT                                                             <  SRR4302540.5434579/94‑1 (MQ=60)
                                 TTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCT                                                             >  SRR4302540.3491724/1‑94 (MQ=60)
                                  TTTGAAGGCGTAACGCCAAATCTCGACCCGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTG                                                            <  SRR4302540.4709000/94‑1 (MQ=60)
                                  TTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTG                                                            >  SRR4302540.1806464/1‑94 (MQ=60)
                                    TGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAA                                                          <  SRR4302540.7288020/94‑1 (MQ=60)
                                    TGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAA                                                          <  SRR4302540.2006283/94‑1 (MQ=60)
                                    TGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAA                                                          >  SRR4302540.8761272/1‑94 (MQ=60)
                                     TAAGGCGCAACGCCCAATCTCGAAACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAAC                                                         <  SRR4302540.3516841/94‑1 (MQ=60)
                                     GAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTAtatgagaaagatgaacgtgctgcac                                                         >  SRR4302540.9174979/1‑69 (MQ=60)
                                     GAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAAC                                                         <  SRR4302540.832078/94‑1 (MQ=60)
                                      AAGGCGTAACTCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTTACCTCGTACATGGTAAAGATGACGTTGCTGAACT                                                        <  SRR4302540.1379230/94‑1 (MQ=60)
                                      AAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACT                                                        <  SRR4302540.1379229/94‑1 (MQ=60)
                                      AAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACT                                                        <  SRR4302540.7589731/94‑1 (MQ=60)
                                      AAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACT                                                        >  SRR4302540.1194914/1‑94 (MQ=60)
                                      AAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACT                                                        <  SRR4302540.7043222/94‑1 (MQ=60)
                                      AAGCCGTACCGCCAAGTCTCGACACGATTGTTGCGCTGCCTGTGCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACT                                                        <  SRR4302540.7589730/94‑1 (MQ=60)
                                       AGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTG                                                       <  SRR4302540.4185901/94‑1 (MQ=60)
                                       AGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTG                                                       >  SRR4302540.6965220/1‑94 (MQ=60)
                                       AGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTG                                                       <  SRR4302540.3805233/94‑1 (MQ=60)
                                       AGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTG                                                       >  SRR4302540.7066535/1‑94 (MQ=60)
                                        GGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGC                                                      >  SRR4302540.7471922/1‑94 (MQ=60)
                                         GCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCA                                                     <  SRR4302540.9507311/94‑1 (MQ=60)
                                         GCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCA                                                     >  SRR4302540.312310/1‑94 (MQ=60)
                                          CGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCAC                                                    >  SRR4302540.62348/1‑94 (MQ=60)
                                           GTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACA                                                   >  SRR4302540.5350473/1‑94 (MQ=60)
                                           GTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACA                                                   >  SRR4302540.9531489/1‑94 (MQ=60)
                                           GTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACA                                                   >  SRR4302540.9513478/1‑94 (MQ=60)
                                           GTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACA                                                   >  SRR4302540.9630041/1‑94 (MQ=60)
                                            TAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAA                                                  <  SRR4302540.6533214/94‑1 (MQ=60)
                                            TAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAA                                                  <  SRR4302540.2251058/94‑1 (MQ=60)
                                             AACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAG                                                 >  SRR4302540.2232114/1‑94 (MQ=60)
                                              ACGCCAAATCTCGACACGATTACTGCTCTGCCTGCTCATGGTCTGCGTCATGACGTCGTACATGGCAAAGATcactgtgctcaagcgcaaaatc                                                >  SRR4302540.4065683/1‑72 (MQ=24)
                                              ACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGC                                                <  SRR4302540.47954/94‑1 (MQ=60)
                                              ACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGC                                                <  SRR4302540.7245849/94‑1 (MQ=60)
                                              ACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGC                                                >  SRR4302540.4065965/1‑94 (MQ=60)
                                               CGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCG                                               <  SRR4302540.6200647/94‑1 (MQ=60)
                                               CGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCG                                               <  SRR4302540.8793057/94‑1 (MQ=60)
                                               CGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCG                                               >  SRR4302540.9825189/1‑94 (MQ=60)
                                                GCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGC                                              >  SRR4302540.7125068/1‑94 (MQ=60)
                                                GCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGC                                              >  SRR4302540.1353197/1‑94 (MQ=60)
                                                GCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTCCACAAGCGC                                              >  SRR4302540.1353196/1‑94 (MQ=60)
                                                CCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGC                                              <  SRR4302540.1306486/94‑1 (MQ=60)
                                                 CCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCC                                             <  SRR4302540.6564976/94‑1 (MQ=60)
                                                 CCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCC                                             >  SRR4302540.4945829/1‑94 (MQ=60)
                                                 CCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCC                                             >  SRR4302540.571740/1‑94 (MQ=60)
                                                  CAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCT                                            >  SRR4302540.122400/1‑94 (MQ=60)
                                                  CAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCT                                            >  SRR4302540.6645476/1‑94 (MQ=60)
                                                  CAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCT                                            >  SRR4302540.7582372/1‑94 (MQ=60)
                                                     ATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCC                                         >  SRR4302540.7979182/1‑94 (MQ=60)
                                                     ATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCC                                         >  SRR4302540.2900372/1‑94 (MQ=60)
                                                      TCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCT                                        >  SRR4302540.1258537/1‑94 (MQ=60)
                                                       CTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTT                                       <  SRR4302540.1466042/94‑1 (MQ=60)
                                                        TCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGTTGAACTGCACAAGCGCCTGCCTTC                                      >  SRR4302540.1685553/1‑94 (MQ=60)
                                                        TCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTC                                      <  SRR4302540.2612173/94‑1 (MQ=60)
                                                        TCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCATGTTGAACTCGTACATGGTAAAGATGAAGTTTCTGAACTccccaagagcccgcattt                                      >  SRR4302540.8206689/1‑76 (MQ=60)
                                                         CGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCT                                     <  SRR4302540.5277141/94‑1 (MQ=60)
                                                         CGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCT                                     <  SRR4302540.286872/94‑1 (MQ=60)
                                                          GACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTG                                    >  SRR4302540.5448522/1‑94 (MQ=60)
                                                          GACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTG                                    >  SRR4302540.770232/1‑94 (MQ=60)
                                                           ACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGA                                   >  SRR4302540.6391423/1‑94 (MQ=60)
                                                            CACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGAC                                  >  SRR4302540.8385340/1‑94 (MQ=60)
                                                            CACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGAC                                  >  SRR4302540.2346956/1‑94 (MQ=60)
                                                            CACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGAC                                  <  SRR4302540.2135227/94‑1 (MQ=60)
                                                            CACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGAC                                  >  SRR4302540.7924615/1‑94 (MQ=60)
                                                             ACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACT                                 <  SRR4302540.2459173/94‑1 (MQ=60)
                                                             ACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACT                                 <  SRR4302540.388284/94‑1 (MQ=60)
                                                             ACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACT                                 <  SRR4302540.2124395/94‑1 (MQ=60)
                                                               GATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGG                               >  SRR4302540.875257/1‑94 (MQ=60)
                                                                   ACTGCGCTGCCTGTTCAGGGTCTGCATGTTGACCTCGTACATGGTAAAGATGACGTTTCTGAACTGCACAAGCGCCTGCCTTCTGACTGGCTGC                           <  SRR4302540.2258294/94‑1 (MQ=60)
                                                                    CTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCT                          >  SRR4302540.533269/1‑94 (MQ=60)
                                                                    CTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCT                          <  SRR4302540.7631681/94‑1 (MQ=60)
                                                                     TGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTG                         >  SRR4302540.8480279/1‑94 (MQ=60)
                                                                     TGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTG                         >  SRR4302540.1615630/1‑94 (MQ=60)
                                                                      GCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGT                        >  SRR4302540.9664250/1‑94 (MQ=60)
                                                                       CGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTC                       >  SRR4302540.9242903/1‑94 (MQ=60)
                                                                       CGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTC                       <  SRR4302540.5566776/94‑1 (MQ=60)
                                                                       CGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTC                       >  SRR4302540.3783574/1‑94 (MQ=60)
                                                                        GCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCT                      <  SRR4302540.1695109/94‑1 (MQ=60)
                                                                        GCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCT                      >  SRR4302540.836584/1‑94 (MQ=60)
                                                                         CTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTG                     >  SRR4302540.921422/1‑94 (MQ=60)
                                                                          TGCCTGTTCTGGGTCTGCGTGTTGCCCTCGTACATGGTAAAGCTGCCGTTGCTGACCTGCACCAGCGCCTGCCTTCTGACTGGTTGCTGTCTGC                    <  SRR4302540.7780713/94‑1 (MQ=60)
                                                                          TGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGC                    <  SRR4302540.8006191/94‑1 (MQ=60)
                                                                           GCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCG                   <  SRR4302540.7366949/94‑1 (MQ=60)
                                                                           GCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCG                   >  SRR4302540.6658509/1‑94 (MQ=60)
                                                                           GCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCG                   <  SRR4302540.8620647/94‑1 (MQ=60)
                                                                             CTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGG                 >  SRR4302540.2548138/1‑94 (MQ=60)
                                                                             CTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGG                 >  SRR4302540.6402812/1‑94 (MQ=60)
                                                                             CTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGG                 >  SRR4302540.2306131/1‑94 (MQ=60)
                                                                                TTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCT              <  SRR4302540.9232206/94‑1 (MQ=60)
                                                                                TTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCT              >  SRR4302540.9282163/1‑94 (MQ=60)
                                                                                TTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCT              >  SRR4302540.1042835/1‑94 (MQ=60)
                                                                                 TCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTG             >  SRR4302540.423362/1‑94 (MQ=60)
                                                                                 TCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTG             <  SRR4302540.4501330/94‑1 (MQ=60)
                                                                                 TCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTG             <  SRR4302540.9221140/94‑1 (MQ=60)
                                                                                  CAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGA            >  SRR4302540.4105697/1‑94 (MQ=60)
                                                                                   AGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGAT           <  SRR4302540.3741927/94‑1 (MQ=60)
                                                                                    GGGTCTGCGTGTTGACCTCGTACATGGTAAAGTTGACGTTGCTGACCTGCACAAGGGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATC          >  SRR4302540.4293900/1‑94 (MQ=60)
                                                                                    GGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATC          >  SRR4302540.8149561/1‑94 (MQ=60)
                                                                                     GGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCA         <  SRR4302540.7193433/94‑1 (MQ=60)
                                                                                      GTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAA        >  SRR4302540.8765441/1‑94 (MQ=60)
                                                                                       TCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAAT       <  SRR4302540.4018768/94‑1 (MQ=60)
                                                                                        CTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCTTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATG      <  SRR4302540.1601694/94‑1 (MQ=60)
                                                                                        CTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATG      >  SRR4302540.2396633/1‑94 (MQ=60)
                                                                                         TGCGTGTTGACCTCGTACCTGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGG     >  SRR4302540.3511411/1‑94 (MQ=60)
                                                                                         TGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGG     >  SRR4302540.4397533/1‑94 (MQ=60)
                                                                                         TGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGG     <  SRR4302540.3393442/94‑1 (MQ=60)
                                                                                         TGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGG     <  SRR4302540.3393443/94‑1 (MQ=60)
                                                                                          GCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGGT    <  SRR4302540.491859/94‑1 (MQ=60)
                                                                                           CGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGGTC   <  SRR4302540.1653587/94‑1 (MQ=60)
                                                                                           CGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGGTC   >  SRR4302540.922445/1‑94 (MQ=60)
                                                                                           CGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGGTC   >  SRR4302540.6042312/1‑94 (MQ=60)
                                                                                            GTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACACGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGGTCG  >  SRR4302540.701348/1‑94 (MQ=60)
                                                                                            GTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGGTCG  >  SRR4302540.3268089/1‑94 (MQ=60)
                                                                                            GTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGGTCG  >  SRR4302540.9543747/1‑94 (MQ=60)
                                                                                            GTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGGTCG  >  SRR4302540.9828223/1‑94 (MQ=60)
                                                                                            |                                                                                             
CCAGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCATGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGGTCG  >  NC_000913/4011338‑4011523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑