Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,011,430 A→G H263R (CAT→CGT)  metE → tetrahydropteroyltriglutamate methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,011,4300AG99.6% 779.7 / ‑5.4 242H263R (CAT→CGT) metEtetrahydropteroyltriglutamate methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (122/119);  minor base C (0/1);  total (122/120)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 254 total aligned reads displayed.

AGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCATGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATG  >  NC_000913/4011340‑4011519
                                                                                          |                                                                                         
 gggACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCgtgt                                                                                        >  2:2854158/1‑93 (MQ=255)
 gggACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCgtgt                                                                                        >  1:6185819/1‑93 (MQ=255)
 gggACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCgtgt                                                                                        >  2:4247962/1‑93 (MQ=255)
 gggACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCgtgt                                                                                        >  2:6041328/1‑93 (MQ=255)
  ggACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGtt                                                                                       >  1:502707/1‑93 (MQ=255)
  ggACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGtt                                                                                       >  1:413384/1‑93 (MQ=255)
   gACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTg                                                                                      >  2:2651232/1‑93 (MQ=255)
    aCAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGa                                                                                     >  2:402541/1‑93 (MQ=255)
    aCAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGa                                                                                     <  1:1588900/93‑1 (MQ=255)
    aCAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGa                                                                                     >  2:3836082/1‑93 (MQ=255)
    aCAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGa                                                                                     <  2:1493645/93‑1 (MQ=255)
      aGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGAcc                                                                                   >  1:5517188/1‑93 (MQ=255)
         tGAAGCTGCTGCTGACCACCTATTGTGAAGGCGTAACGCCAAATCGCGACGCGATGACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCg                                                                                <  1:408519/93‑1 (MQ=255)
           aacctgctgctgACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTCCTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTa                                                                              <  2:5437897/90‑1 (MQ=255)
           aaaCTGCTTCTGACTACCTATTTTGAAGTCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGTCCTCGTa                                                                              <  1:2248455/93‑1 (MQ=255)
           aaaCTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTa                                                                              >  2:2228603/1‑93 (MQ=255)
           aaaCTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTa                                                                              <  1:2768931/93‑1 (MQ=255)
              ctgctgctgACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACAt                                                                           <  2:3059315/93‑1 (MQ=255)
              ctgctgctgACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACAt                                                                           >  2:1227130/1‑93 (MQ=255)
              ctgctgctgACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACAt                                                                           >  1:1508228/1‑93 (MQ=255)
              ctgctgctgACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACAt                                                                           >  2:4418690/1‑93 (MQ=255)
                gctgctgACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATgg                                                                         <  2:2689327/93‑1 (MQ=255)
                   gctgACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCTTACATGGTaa                                                                      <  2:4174089/93‑1 (MQ=255)
                   gctgA‑CACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTaaa                                                                     <  2:6236274/93‑1 (MQ=255)
                    ctgACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTaaa                                                                     <  1:3413027/93‑1 (MQ=255)
                     tgACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAg                                                                    >  2:3984966/1‑93 (MQ=255)
                      gACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGa                                                                   >  2:4831797/1‑93 (MQ=255)
                       accaccTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGAt                                                                  <  1:5813727/93‑1 (MQ=255)
                        tcaccTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATTGTAAAGATg                                                                 <  2:1046734/92‑1 (MQ=255)
                        ccaccTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATg                                                                 <  2:3135614/93‑1 (MQ=255)
                         caccTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGa                                                                <  2:4546153/93‑1 (MQ=255)
                          accTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGAc                                                               <  1:4973540/93‑1 (MQ=255)
                          accTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGAc                                                               >  2:1859022/1‑93 (MQ=255)
                           ccTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACg                                                              <  2:3759917/93‑1 (MQ=255)
                           ccTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACg                                                              <  2:4683798/93‑1 (MQ=255)
                            cTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGt                                                             >  1:4651227/1‑93 (MQ=255)
                             tATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGtt                                                            <  2:2202818/93‑1 (MQ=255)
                             tATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGtt                                                            <  2:130119/93‑1 (MQ=255)
                             tATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGtt                                                            >  2:5667367/1‑93 (MQ=255)
                                   gAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGaa                                                      >  1:5475165/1‑93 (MQ=255)
                                     aGGCGTATCGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACt                                                    <  2:1528568/93‑1 (MQ=255)
                                        cGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCCTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGca                                                 <  1:5846371/93‑1 (MQ=255)
                                         gTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGcac                                                >  1:3791880/1‑93 (MQ=255)
                                         gTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGcac                                                >  2:5567018/1‑93 (MQ=255)
                                            aCGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAg                                             >  1:3987355/1‑93 (MQ=255)
                                            aCGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAg                                             <  1:2907394/93‑1 (MQ=255)
                                             cGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAgc                                            >  2:5890929/1‑93 (MQ=255)
                                              gCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAgcg                                           >  2:2874519/1‑93 (MQ=255)
                                                cAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCgcc                                         >  2:2478993/1‑93 (MQ=255)
                                                cAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCgcc                                         >  2:779861/1‑93 (MQ=255)
                                                 aaaTCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCgcct                                        <  1:5328553/93‑1 (MQ=255)
                                                   aTCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCgcctgc                                      >  2:358322/1‑93 (MQ=255)
                                                   aTCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCgcctgc                                      >  1:4714170/1‑93 (MQ=255)
                                                     ctcGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCgcctgcct                                    <  2:919972/93‑1 (MQ=255)
                                                      tcGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCtt                                   >  1:3752767/1‑93 (MQ=255)
                                                        gACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCt                                 >  2:5066088/1‑93 (MQ=255)
                                                         acacGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTg                                >  1:418916/1‑93 (MQ=255)
                                                         acacGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTg                                >  1:4492735/1‑93 (MQ=255)
                                                          cacGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGa                               <  1:5774012/93‑1 (MQ=255)
                                                          cacGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGa                               >  1:3241867/1‑93 (MQ=255)
                                                          cacGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGa                               >  2:5397842/1‑93 (MQ=255)
                                                           acGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGAc                              <  2:4757287/93‑1 (MQ=255)
                                                           acGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGAc                              <  2:3914647/93‑1 (MQ=255)
                                                            cGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGAca                             <  1:899774/93‑2 (MQ=255)
                                                            cGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACt                             <  1:773641/93‑1 (MQ=255)
                                                            cGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACt                             <  1:1715249/93‑1 (MQ=255)
                                                            cGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACt                             >  1:4294217/1‑93 (MQ=255)
                                                            cGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACt                             >  1:2332896/1‑93 (MQ=255)
                                                             gATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTg                            >  2:1177017/1‑93 (MQ=255)
                                                               ttACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGt                          <  2:2996619/93‑1 (MQ=255)
                                                                  cTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGc                       <  1:5194651/93‑1 (MQ=255)
                                                                   tGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCt                      >  1:2884048/1‑93 (MQ=255)
                                                                   tGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCt                      <  2:2451552/93‑1 (MQ=255)
                                                                      gcTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTc                   <  2:2082543/93‑1 (MQ=255)
                                                                       cTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCt                  >  1:2304632/1‑93 (MQ=255)
                                                                       cTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCt                  >  1:62957/1‑93 (MQ=255)
                                                                        tGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTg                 <  2:5496179/93‑1 (MQ=255)
                                                                         gCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGc                >  2:2283480/1‑93 (MQ=255)
                                                                         gCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGc                <  1:5924840/93‑1 (MQ=255)
                                                                         gCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGc                >  2:2139064/1‑93 (MQ=255)
                                                                           cTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCgg              <  1:1168715/93‑1 (MQ=255)
                                                                           cTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCgg              >  1:1049326/1‑93 (MQ=255)
                                                                           cTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCgg              >  1:3529478/1‑93 (MQ=255)
                                                                            tGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCggg             >  2:3158653/1‑93 (MQ=255)
                                                                            tGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCggg             >  1:2540100/1‑93 (MQ=255)
                                                                              ttCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTc           <  2:951974/93‑1 (MQ=255)
                                                                              ttCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTc           <  1:5605348/93‑1 (MQ=255)
                                                                               tCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCt          <  2:3695455/93‑1 (MQ=255)
                                                                                cAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTg         <  2:6252215/93‑1 (MQ=255)
                                                                                  gggTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGAt       <  2:5248913/93‑1 (MQ=255)
                                                                                  gggTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGAt       <  1:858802/93‑1 (MQ=255)
                                                                                  gggTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGAt       >  1:2415614/1‑93 (MQ=255)
                                                                                     tCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCaa    <  2:5353016/93‑1 (MQ=255)
                                                                                     tCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCaa    >  1:3107040/1‑93 (MQ=255)
                                                                                     tCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCaa    >  1:2173957/1‑93 (MQ=255)
                                                                                     tCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCaa    >  1:1687903/1‑93 (MQ=255)
                                                                                      cTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAAt   <  1:2030292/93‑1 (MQ=255)
                                                                                      cTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAAt   <  2:1919781/93‑1 (MQ=255)
                                                                                      cTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCATTCTGACTGGTTGCTGTCTGCGGGTCTGATCAAt   <  1:2093972/93‑1 (MQ=255)
                                                                                       tGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATg  >  1:5072032/1‑93 (MQ=255)
                                                                                          |                                                                                         
AGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCATGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATG  >  NC_000913/4011340‑4011519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCCAGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCATGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGGTC  >  NC_000913/4011337‑4011522
                                                                                             |                                                                                            
TCCAGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCG                                                                                              >  SRR4302533.3330641/1‑94 (MQ=60)
TCCAGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCG                                                                                              <  SRR4302533.3683351/94‑1 (MQ=60)
TCCAGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCG                                                                                              >  SRR4302533.4573909/1‑94 (MQ=60)
 CCAGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGT                                                                                             >  SRR4302533.811258/1‑94 (MQ=60)
  CAGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTG                                                                                            <  SRR4302533.2016914/94‑1 (MQ=60)
   AGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGT                                                                                           >  SRR4302533.6067850/1‑94 (MQ=60)
   AGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGT                                                                                           <  SRR4302533.551706/94‑1 (MQ=60)
   AGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGT                                                                                           >  SRR4302533.6222952/1‑94 (MQ=60)
    GGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTT                                                                                          <  SRR4302533.1614931/94‑1 (MQ=60)
    GGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTT                                                                                          >  SRR4302533.2469326/1‑94 (MQ=60)
    GGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTT                                                                                          >  SRR4302533.415865/1‑94 (MQ=60)
    GGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTT                                                                                          >  SRR4302533.5796875/1‑94 (MQ=60)
    GGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTT                                                                                          >  SRR4302533.505695/1‑94 (MQ=60)
      GACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTCA                                                                                        <  SRR4302533.587384/94‑1 (MQ=60)
       ACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGAC                                                                                       <  SRR4302533.1598509/94‑1 (MQ=60)
        CAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACC                                                                                      >  SRR4302533.1703042/1‑94 (MQ=60)
        CAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACC                                                                                      <  SRR4302533.3883443/94‑1 (MQ=60)
        CAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACC                                                                                      >  SRR4302533.5550501/1‑94 (MQ=60)
         AGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCT                                                                                     >  SRR4302533.255853/1‑94 (MQ=60)
            TGAAGCTGCTGCTGACCACCTATTGTGAAGGCGTAACGCCAAATCGCGACGCGATGACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGT                                                                                  <  SRR4302533.410972/94‑1 (MQ=60)
            GGAAACTGCTGCTGACCGCCTATTTTGAAGGCGTAACGCCAAAGCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGT                                                                                  <  SRR4302533.817265/94‑1 (MQ=60)
              AAACTGCTTCTGACTACCTATTTTGAAGTCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGTCCTCGTAC                                                                                <  SRR4302533.2262349/94‑1 (MQ=60)
              AAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTAC                                                                                <  SRR4302533.2785924/94‑1 (MQ=60)
              AAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTAC                                                                                <  SRR4302533.5835781/94‑1 (MQ=60)
              AAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTAC                                                                                <  SRR4302533.5852235/94‑1 (MQ=60)
                ACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACAT                                                                              >  SRR4302533.1517319/1‑94 (MQ=60)
                  TGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGG                                                                            >  SRR4302533.4599384/1‑94 (MQ=60)
                  TGATGCTGACCACCTATTTTGAAGGCGTAACGCAAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGG                                                                            <  SRR4302533.517414/94‑1 (MQ=60)
                    CTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTA                                                                          <  SRR4302533.4862641/94‑1 (MQ=60)
                    CTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTA                                                                          >  SRR4302533.4324938/1‑94 (MQ=60)
                      GCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAA                                                                        >  SRR4302533.5124414/1‑94 (MQ=60)
                       CTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAG                                                                       <  SRR4302533.3434111/94‑1 (MQ=60)
                       CTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAG                                                                       <  SRR4302533.124767/94‑1 (MQ=60)
                         GACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGAT                                                                     <  SRR4302533.2414066/94‑1 (MQ=60)
                         GACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGAT                                                                     <  SRR4302533.1296146/94‑1 (MQ=60)
                         GACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGAT                                                                     <  SRR4302533.683122/94‑1 (MQ=60)
                         GACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGAT                                                                     <  SRR4302533.2107630/94‑1 (MQ=60)
                          ACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATG                                                                    <  SRR4302533.5848839/94‑1 (MQ=60)
                             ACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACG                                                                 <  SRR4302533.5003696/94‑1 (MQ=60)
                              CCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGT                                                                >  SRR4302533.4679409/1‑94 (MQ=60)
                               CTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTT                                                               <  SRR4302533.1134425/94‑1 (MQ=60)
                               CTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTT                                                               >  SRR4302533.2085607/1‑94 (MQ=60)
                                TATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTG                                                              <  SRR4302533.4729197/94‑1 (MQ=60)
                                TATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTG                                                              >  SRR4302533.5036373/1‑94 (MQ=60)
                                CATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTG                                                              <  SRR4302533.6083498/94‑1 (MQ=60)
                                   TTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTG                                                           <  SRR4302533.2203186/94‑1 (MQ=60)
                                   TTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTG                                                           <  SRR4302533.3780020/94‑1 (MQ=60)
                                   TTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTG                                                           <  SRR4302533.5917172/94‑1 (MQ=60)
                                    TTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGA                                                          >  SRR4302533.1618578/1‑94 (MQ=60)
                                     TGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAA                                                         >  SRR4302533.5508167/1‑94 (MQ=60)
                                     TGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAA                                                         >  SRR4302533.2289169/1‑94 (MQ=60)
                                        AGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTG                                                      <  SRR4302533.3419677/94‑1 (MQ=60)
                                           CGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCAC                                                   >  SRR4302533.3815093/1‑94 (MQ=60)
                                           CGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCAC                                                   >  SRR4302533.2813184/1‑94 (MQ=60)
                                           CGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCCTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCAC                                                   <  SRR4302533.5881716/94‑1 (MQ=60)
                                              AACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAG                                                >  SRR4302533.4011644/1‑94 (MQ=60)
                                               ACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGC                                               >  SRR4302533.5320741/1‑94 (MQ=60)
                                               ACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGC                                               <  SRR4302533.2925243/94‑1 (MQ=60)
                                                CGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCG                                              <  SRR4302533.4186360/94‑1 (MQ=60)
                                                 GCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGC                                             >  SRR4302533.4688191/1‑94 (MQ=60)
                                                  CCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCC                                            >  SRR4302533.699937/1‑94 (MQ=60)
                                                  CCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCC                                            >  SRR4302533.42252/1‑94 (MQ=60)
                                                  CCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCC                                            <  SRR4302533.4211551/94‑1 (MQ=60)
                                                   CAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCT                                           >  SRR4302533.999873/1‑94 (MQ=60)
                                                   CAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCT                                           <  SRR4302533.439549/94‑1 (MQ=60)
                                                   CAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCT                                           >  SRR4302533.3519369/1‑94 (MQ=60)
                                                   CAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCT                                           >  SRR4302533.3236821/1‑94 (MQ=60)
                                                    AAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTG                                          <  SRR4302533.5360487/94‑1 (MQ=60)
                                                    AAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTG                                          >  SRR4302533.5627250/1‑94 (MQ=60)
                                                     AATCTCGACACGATTACTGTGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGC                                         <  SRR4302533.2820889/94‑1 (MQ=60)
                                                     AATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGC                                         >  SRR4302533.4742742/1‑94 (MQ=60)
                                                      ATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCC                                        >  SRR4302533.2291767/1‑94 (MQ=60)
                                                      ATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCC                                        >  SRR4302533.3038964/1‑94 (MQ=60)
                                                       TCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCT                                       >  SRR4302533.5068233/1‑94 (MQ=60)
                                                       TCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCT                                       <  SRR4302533.3661877/94‑1 (MQ=60)
                                                        CTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTT                                      >  SRR4302533.3775771/1‑94 (MQ=60)
                                                        CTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTT                                      >  SRR4302533.5383511/1‑94 (MQ=60)
                                                        CTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTT                                      >  SRR4302533.4067606/1‑94 (MQ=60)
                                                         TCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTC                                     <  SRR4302533.2387304/94‑1 (MQ=60)
                                                         TCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTC                                     >  SRR4302533.3356634/1‑94 (MQ=60)
                                                          CGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCT                                    <  SRR4302533.2120050/94‑1 (MQ=60)
                                                           GACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTG                                   >  SRR4302533.421424/1‑94 (MQ=60)
                                                           GACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTG                                   >  SRR4302533.4519945/1‑94 (MQ=60)
                                                            ACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGA                                  >  SRR4302533.3261847/1‑94 (MQ=60)
                                                             CACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGAC                                 <  SRR4302533.5808884/94‑1 (MQ=60)
                                                              ACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACT                                >  SRR4302533.4320043/1‑94 (MQ=60)
                                                              ACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACT                                >  SRR4302533.2347340/1‑94 (MQ=60)
                                                               CGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTG                               <  SRR4302533.1725648/94‑1 (MQ=60)
                                                               CGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTG                               <  SRR4302533.778190/94‑1 (MQ=60)
                                                               CGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACAG                               <  SRR4302533.905054/94‑1 (MQ=60)
                                                                 ATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGT                             <  SRR4302533.1561582/94‑1 (MQ=60)
                                                                   TACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTG                           >  SRR4302533.2337963/1‑94 (MQ=60)
                                                                     CTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGCACTGCACAAGACCCTGCCTTCTGACTGGTTGCT                         >  SRR4302533.2901785/1‑94 (MQ=60)
                                                                     CTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCT                         <  SRR4302533.5225983/94‑1 (MQ=60)
                                                                     CTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCT                         <  SRR4302533.562206/94‑1 (MQ=60)
                                                                     CTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCT                         >  SRR4302533.2901784/1‑94 (MQ=60)
                                                                     CTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCT                         <  SRR4302533.897332/94‑1 (MQ=60)
                                                                       GCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGT                       >  SRR4302533.4121562/1‑94 (MQ=60)
                                                                         GCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCT                     >  SRR4302533.63370/1‑94 (MQ=60)
                                                                         GCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCT                     >  SRR4302533.2318908/1‑94 (MQ=60)
                                                                            GCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCG                  <  SRR4302533.4116161/94‑1 (MQ=60)
                                                                            GCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCG                  <  SRR4302533.5960658/94‑1 (MQ=60)
                                                                             CCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGG                 >  SRR4302533.1055494/1‑94 (MQ=60)
                                                                             CCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGG                 <  SRR4302533.3092218/94‑1 (MQ=60)
                                                                             CCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGG                 >  SRR4302533.3551229/1‑94 (MQ=60)
                                                                             CCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGG                 <  SRR4302533.1855062/94‑1 (MQ=60)
                                                                              CTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGG                >  SRR4302533.2555737/1‑94 (MQ=60)
                                                                              CTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGG                >  SRR4302533.3888471/1‑94 (MQ=60)
                                                                              CTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGG                <  SRR4302533.5808046/94‑1 (MQ=60)
                                                                              CTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGG                <  SRR4302533.1175726/94‑1 (MQ=60)
                                                                               TGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGT               <  SRR4302533.1730748/94‑1 (MQ=60)
                                                                               TGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGT               >  SRR4302533.870055/1‑94 (MQ=60)
                                                                                GTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTC              >  SRR4302533.6261251/1‑94 (MQ=60)
                                                                                 TTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCT             <  SRR4302533.5639218/94‑1 (MQ=60)
                                                                                  TCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTG            <  SRR4302533.3560720/94‑1 (MQ=60)
                                                                                    AGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGAT          >  SRR4302533.2430537/1‑94 (MQ=60)
                                                                                     GGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATC         <  SRR4302533.863834/94‑1 (MQ=60)
                                                                                      GGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCA        <  SRR4302533.501608/94‑1 (MQ=60)
                                                                                       GTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAA       >  SRR4302533.1698132/1‑94 (MQ=60)
                                                                                       GTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAA       >  SRR4302533.3126136/1‑94 (MQ=60)
                                                                                       GTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAA       >  SRR4302533.2187353/1‑94 (MQ=60)
                                                                                         CTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATG     <  SRR4302533.2042823/94‑1 (MQ=60)
                                                                                         CTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATG     >  SRR4302533.5480353/1‑94 (MQ=60)
                                                                                         CTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATG     >  SRR4302533.5102689/1‑94 (MQ=60)
                                                                                         CTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATG     >  SRR4302533.2498906/1‑94 (MQ=60)
                                                                                         CTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCATTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATG     <  SRR4302533.2106867/94‑1 (MQ=60)
                                                                                          TGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGG    >  SRR4302533.1639098/1‑94 (MQ=60)
                                                                                           GCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGGT   <  SRR4302533.857/94‑1 (MQ=60)
                                                                                           GCGTGTTGACCTCCTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGGT   <  SRR4302533.4018238/94‑1 (MQ=60)
                                                                                            CGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGGTC  >  SRR4302533.4481746/1‑94 (MQ=60)
                                                                                            CGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGGTC  <  SRR4302533.4362797/94‑1 (MQ=60)
                                                                                             |                                                                                            
TCCAGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCATGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGGTC  >  NC_000913/4011337‑4011522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑