Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,032,327 A→G Q44R (CAG→CGG)  hemG → protoporphyrin oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,032,3270AG99.8% 1465.5 / ‑5.0 452Q44R (CAG→CGG) hemGprotoporphyrin oxidase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (209/242);  total (209/243)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.63e-01

Only 100 of 466 total aligned reads displayed.

CGCGCGAGATTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACAGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATG  >  NC_000913/4032237‑4032416
                                                                                          |                                                                                         
  cgcgAGATTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTgg                                                                                       <  1:6964597/93‑1 (MQ=255)
      agaTTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGaaa                                                                                   >  1:923008/1‑93 (MQ=255)
      agaTTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGaaa                                                                                   >  2:8970401/1‑93 (MQ=255)
        aTTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAAc                                                                                 >  1:6065679/1‑93 (MQ=255)
          tGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTa                                                                               <  1:1217752/93‑1 (MQ=255)
          tGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTa                                                                               <  1:3346115/93‑1 (MQ=255)
           gCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGAGGGAAAACtct                                                                              >  2:2226346/1‑91 (MQ=255)
            cctcctACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATg                                                                             >  1:6570143/1‑93 (MQ=255)
             ctcctACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGa                                                                            >  2:9398355/1‑93 (MQ=255)
              tcctACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGAc                                                                           >  2:4166674/1‑93 (MQ=255)
              tcctACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGAc                                                                           >  2:6821396/1‑93 (MQ=255)
              tcctACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGAc                                                                           <  2:6471603/93‑1 (MQ=255)
              tcctACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGAc                                                                           >  1:7282594/1‑93 (MQ=255)
               cctACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGAcc                                                                          <  1:800992/93‑1 (MQ=255)
               cctACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGAcc                                                                          >  2:3199167/1‑93 (MQ=255)
                ctACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCg                                                                         >  1:7780735/1‑93 (MQ=255)
                ctACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCg                                                                         >  2:9642312/1‑93 (MQ=255)
                ctACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCg                                                                         >  1:9807245/1‑93 (MQ=255)
                 tACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCgt                                                                        >  2:4859983/1‑93 (MQ=255)
                 tACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCgt                                                                        >  2:7527929/1‑93 (MQ=255)
                 tACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCgt                                                                        >  1:9171092/1‑93 (MQ=255)
                 tACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCgt                                                                        >  1:4712328/1‑93 (MQ=255)
                    cTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGtgtg                                                                     >  2:6081854/1‑93 (MQ=255)
                     tGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTgg                                                                    >  1:5234716/1‑93 (MQ=255)
                     tGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTgg                                                                    <  1:9475014/93‑1 (MQ=255)
                        cTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCa                                                                 <  2:9571064/93‑1 (MQ=255)
                        cTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCa                                                                 <  1:2804982/93‑1 (MQ=255)
                         ttCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCAt                                                                >  2:2588250/1‑93 (MQ=255)
                         ttCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCAt                                                                >  2:8401164/1‑93 (MQ=255)
                          tCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTCTGACCGTGTGGTCAtt                                                               >  2:8003865/1‑93 (MQ=255)
                           cGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTg                                                              >  1:4042521/1‑93 (MQ=255)
                            ggAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTgg                                                             <  1:7134815/93‑1 (MQ=255)
                             gAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGt                                                            <  2:6992551/93‑1 (MQ=255)
                              aaCTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTg                                                           >  2:5409986/1‑93 (MQ=255)
                               aCTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGc                                                          <  1:1055788/93‑1 (MQ=255)
                                cTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCt                                                         >  2:8788487/1‑93 (MQ=255)
                                 tGAAAGAACTGGGGATCCAGTCGGATGTCGCCAATGTGCACCGCATTGAAGATCCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCtt                                                        <  2:3678927/93‑1 (MQ=255)
                                 tGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGAtt                                                        >  2:8331406/1‑93 (MQ=255)
                                   aaaGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCt                                                      >  1:4191451/1‑93 (MQ=255)
                                   aaaGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCt                                                      <  1:2744002/93‑1 (MQ=255)
                                    aaGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTa                                                     >  1:4303311/1‑93 (MQ=255)
                                     aGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTAt                                                    <  1:4946173/93‑1 (MQ=255)
                                     aGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGGGTGGTCATTGGTGCTTCTAt                                                    >  2:8565890/1‑93 (MQ=255)
                                      gAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTAtt                                                   <  2:5174303/93‑1 (MQ=255)
                                       gaCTGGGGAGGCGGGGGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAACCTATGACCGTGTGGTCATTGGTGCTTCTATTc                                                  <  1:5103114/92‑1 (MQ=255)
                                       aaCTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTc                                                  <  1:3559137/93‑1 (MQ=255)
                                         cTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGc                                                >  2:1386564/1‑93 (MQ=255)
                                         cTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGc                                                >  1:9036884/1‑93 (MQ=255)
                                           ggggATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTa                                              <  1:10011987/93‑1 (MQ=255)
                                           ggggAGCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTa                                              <  2:5703178/93‑1 (MQ=255)
                                            gggATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTAt                                             <  2:4629472/93‑1 (MQ=255)
                                              gATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATgg                                           <  1:8362473/93‑1 (MQ=255)
                                                tCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTc                                         <  2:5906150/93‑1 (MQ=255)
                                                tCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTc                                         >  1:8189228/1‑93 (MQ=255)
                                                tCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTc                                         <  2:2351066/93‑1 (MQ=255)
                                                 ccAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCa                                        >  1:5512601/1‑93 (MQ=255)
                                                   aGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACt                                      >  2:9797702/1‑93 (MQ=255)
                                                    ggCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTa                                     >  1:2347628/1‑93 (MQ=255)
                                                    ggCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTa                                     >  2:7891288/1‑93 (MQ=255)
                                                       ggATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCa                                  <  2:2155613/93‑1 (MQ=255)
                                                       ggATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCa                                  <  2:8169120/93‑1 (MQ=255)
                                                        gATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCAt                                 >  1:368052/1‑93 (MQ=255)
                                                        gATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCAt                                 >  1:4645380/1‑93 (MQ=255)
                                                        gATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCAt                                 >  1:7493212/1‑93 (MQ=255)
                                                         aTGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCAtt                                <  1:1672446/93‑1 (MQ=255)
                                                           gTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCa                              <  1:6632153/93‑1 (MQ=255)
                                                            tCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAg                             >  2:4089453/1‑93 (MQ=255)
                                                             cGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAgt                            <  1:9100526/93‑2 (MQ=255)
                                                               ccAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAgtgt                          >  2:159383/1‑93 (MQ=255)
                                                               ccAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAgtgt                          <  2:623139/93‑1 (MQ=255)
                                                                cAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGtt                         >  2:10001692/1‑93 (MQ=255)
                                                                   tgtgCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCa                      >  2:1825800/1‑93 (MQ=255)
                                                                   tgtgCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCa                      >  1:2448111/1‑93 (MQ=255)
                                                                   tgtgCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCa                      <  2:3418299/93‑1 (MQ=255)
                                                                   tgtgCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCa                      >  2:1982412/1‑93 (MQ=255)
                                                                   tgtgCACCGCATTGAAGAACCACGGGGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTccg                      >  2:1132647/1‑92 (MQ=255)
                                                                         ccGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAAtt                <  2:1706278/93‑1 (MQ=255)
                                                                         ccGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAAtt                >  2:1681030/1‑93 (MQ=255)
                                                                          cGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAAttt               <  2:267119/93‑1 (MQ=255)
                                                                          cGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAAttt               <  2:2492221/93‑1 (MQ=255)
                                                                            cATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGt             <  1:6242658/93‑1 (MQ=255)
                                                                             aTTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTc            >  1:2998175/1‑93 (MQ=255)
                                                                              ttGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCa           >  1:9600259/1‑93 (MQ=255)
                                                                                   gaaCCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCaaaaaa      >  2:7630524/1‑93 (MQ=255)
                                                                                   gaaCCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCaaaaaa      >  1:8842258/1‑93 (MQ=255)
                                                                                    aaCCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCaaaaaaa     >  1:2098114/1‑92 (MQ=255)
                                                                                    aaCCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAAc     <  1:823561/93‑1 (MQ=255)
                                                                                     aCCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACa    <  2:7285619/93‑1 (MQ=255)
                                                                                     aCCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACa    <  2:9282322/93‑1 (MQ=255)
                                                                                     aCCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACa    <  2:2892106/93‑1 (MQ=255)
                                                                                     aCCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACa    <  1:1366750/93‑1 (MQ=255)
                                                                                     aCCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACa    <  2:4317174/93‑1 (MQ=255)
                                                                                      ccACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACAt   >  1:5691363/1‑93 (MQ=255)
                                                                                      ccACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACAt   >  1:5900764/1‑93 (MQ=255)
                                                                                      ccACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACAt   >  2:6555375/1‑93 (MQ=255)
                                                                                      ccACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACAt   <  1:8643477/93‑1 (MQ=255)
                                                                                      ccACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACAt   >  2:4908225/1‑93 (MQ=255)
                                                                                      ccACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACAt   <  1:8549537/93‑1 (MQ=255)
                                                                                       cacgGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATg  >  2:9050252/1‑93 (MQ=255)
                                                                                       cacgGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATg  <  2:9203478/93‑1 (MQ=255)
                                                                                          |                                                                                         
CGCGCGAGATTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACAGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATG  >  NC_000913/4032237‑4032416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 247 total aligned reads displayed.
AAACGCGCGAGATTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACAGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGAC  >  NC_000913/4032234‑4032420
                                                                                             |                                                                                             
CAACGCGCGAGATTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACG                                                                                               <  SRR4302540.3445799/94‑1 (MQ=60)
AAACGCGCGAGATTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACG                                                                                               >  SRR4302540.8892994/1‑94 (MQ=60)
 AACGCGCGAGATTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGG                                                                                              <  SRR4302540.3482304/94‑1 (MQ=60)
 AACGCGCGAGATTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGG                                                                                              <  SRR4302540.2362988/94‑1 (MQ=60)
  ACGCGCGAGATTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGT                                                                                             <  SRR4302540.7850873/94‑1 (MQ=60)
    GCGCGAGATTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGG                                                                                           <  SRR4302540.4772977/94‑1 (MQ=60)
    GCGCGAGATTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGG                                                                                           <  SRR4302540.238046/94‑1 (MQ=60)
    GCGCGAGATGTCCCCCTACCTGGGTTCGGAACTGAAAGAACTGGAAATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGG                                                                                           <  SRR4302540.7523252/94‑1 (MQ=60)
     CGCGAGATTGCCTCCTGCCTGGCTTCGGATCTGAAAGAACTGGGGATCCAGGCTGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGG                                                                                          <  SRR4302540.10078388/94‑1 (MQ=60)
     CGCGAGATTGCCTCCTACCTGTCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGG                                                                                          <  SRR4302540.2805783/94‑1 (MQ=60)
     CGCGAGATTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGG                                                                                          <  SRR4302540.7042045/94‑1 (MQ=60)
     CGCGAGATTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGG                                                                                          >  SRR4302540.9511348/1‑94 (MQ=60)
      GCGAGATTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGA                                                                                         >  SRR4302540.4526135/1‑94 (MQ=60)
       CGAGATTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAA                                                                                        >  SRR4302540.9650230/1‑94 (MQ=60)
        GAGATTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAA                                                                                       >  SRR4302540.932740/1‑94 (MQ=60)
        GAGATTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAA                                                                                       <  SRR4302540.9331631/94‑1 (MQ=60)
         AGATTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAA                                                                                      <  SRR4302540.364281/94‑1 (MQ=60)
         AGATTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAA                                                                                      >  SRR4302540.5484211/1‑94 (MQ=60)
          GATTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAAC                                                                                     <  SRR4302540.5037240/94‑1 (MQ=60)
          GATTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAAC                                                                                     >  SRR4302540.6059687/1‑94 (MQ=60)
          GATTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAAC                                                                                     >  SRR4302540.6133112/1‑94 (MQ=60)
          GATTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAAC                                                                                     <  SRR4302540.9240369/94‑1 (MQ=60)
           ATTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACT                                                                                    <  SRR4302540.24384/94‑1 (MQ=60)
             TGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTAT                                                                                  <  SRR4302540.3382821/94‑1 (MQ=60)
             TGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTAT                                                                                  <  SRR4302540.5264905/94‑1 (MQ=60)
             TGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTAT                                                                                  <  SRR4302540.2675046/94‑1 (MQ=60)
             TGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTAT                                                                                  <  SRR4302540.1230220/94‑1 (MQ=60)
              GCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATG                                                                                 >  SRR4302540.6642941/1‑94 (MQ=60)
                CTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGACGAACCAAGTTGGGAAAACTATGAC                                                                               >  SRR4302540.2922877/1‑94 (MQ=60)
                CTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGAC                                                                               >  SRR4302540.5109675/1‑94 (MQ=60)
                CTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGAC                                                                               <  SRR4302540.5349644/94‑1 (MQ=60)
                CTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGAC                                                                               >  SRR4302540.7363864/1‑94 (MQ=60)
                CTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGAC                                                                               >  SRR4302540.8994295/1‑94 (MQ=60)
                 TCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCCTTGAAGAACCACGGTGGGAAAACTATGACC                                                                              >  SRR4302540.3563541/1‑94 (MQ=60)
                 TCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACC                                                                              <  SRR4302540.1277522/94‑1 (MQ=60)
                 TCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACC                                                                              <  SRR4302540.3444337/94‑1 (MQ=60)
                 TCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACC                                                                              <  SRR4302540.5787679/94‑1 (MQ=60)
                 CCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACC                                                                              <  SRR4302540.365900/94‑1 (MQ=60)
                  CCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCG                                                                             <  SRR4302540.6255485/94‑1 (MQ=60)
                  CCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCG                                                                             >  SRR4302540.9922038/1‑94 (MQ=60)
                  CCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCG                                                                             <  SRR4302540.809500/94‑1 (MQ=60)
                   CTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGT                                                                            >  SRR4302540.5195305/1‑94 (MQ=60)
                   CTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGT                                                                            >  SRR4302540.9281107/1‑94 (MQ=60)
                   CTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGT                                                                            >  SRR4302540.7042663/1‑94 (MQ=60)
                   CTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGT                                                                            >  SRR4302540.9227065/1‑94 (MQ=60)
                    TACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTG                                                                           >  SRR4302540.3956735/1‑94 (MQ=60)
                     ACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGT                                                                          <  SRR4302540.9301457/94‑1 (MQ=60)
                       CTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGG                                                                        <  SRR4302540.288735/94‑1 (MQ=60)
                       CTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGG                                                                        >  SRR4302540.8275521/1‑94 (MQ=60)
                        TGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGT                                                                       <  SRR4302540.9587386/94‑1 (MQ=60)
                         GGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTC                                                                      <  SRR4302540.4888965/94‑1 (MQ=60)
                           CTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCAT                                                                    <  SRR4302540.8692081/94‑1 (MQ=60)
                           CTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCAT                                                                    <  SRR4302540.8129217/94‑1 (MQ=60)
                           CTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCAT                                                                    <  SRR4302540.8294014/94‑1 (MQ=60)
                           CTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCAT                                                                    <  SRR4302540.5474404/94‑1 (MQ=60)
                            TTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATT                                                                   <  SRR4302540.9521292/94‑1 (MQ=60)
                            TTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATT                                                                   >  SRR4302540.1561448/1‑94 (MQ=60)
                             TCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTG                                                                  <  SRR4302540.3135134/94‑1 (MQ=60)
                             TCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTG                                                                  <  SRR4302540.4760868/94‑1 (MQ=60)
                             TCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTG                                                                  >  SRR4302540.4086097/1‑94 (MQ=60)
                             TCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTG                                                                  <  SRR4302540.4089081/94‑1 (MQ=60)
                              CGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGG                                                                 <  SRR4302540.9224155/94‑1 (MQ=60)
                              CGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGG                                                                 <  SRR4302540.8338025/94‑1 (MQ=60)
                               GGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGT                                                                <  SRR4302540.7214308/94‑1 (MQ=60)
                                GAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTG                                                               <  SRR4302540.7503408/94‑1 (MQ=60)
                                GAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTG                                                               >  SRR4302540.4885961/1‑94 (MQ=60)
                                GAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTG                                                               >  SRR4302540.895712/1‑94 (MQ=60)
                                GAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTG                                                               >  SRR4302540.8458492/1‑94 (MQ=60)
                                 AACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGC                                                              <  SRR4302540.6296612/94‑1 (MQ=60)
                                  ACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCT                                                             <  SRR4302540.1448036/94‑1 (MQ=60)
                                  ACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCT                                                             <  SRR4302540.1066531/94‑1 (MQ=60)
                                  ACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCT                                                             >  SRR4302540.8836352/1‑94 (MQ=60)
                                   CTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTT                                                            >  SRR4302540.9673193/1‑94 (MQ=60)
                                   CTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTT                                                            >  SRR4302540.6230184/1‑94 (MQ=60)
                                    TGAAAGAACTGGGGATCCAGGCTCATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTC                                                           >  SRR4302540.1849427/1‑94 (MQ=60)
                                    TGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTC                                                           >  SRR4302540.9833306/1‑94 (MQ=60)
                                     GAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCT                                                          >  SRR4302540.4237040/1‑94 (MQ=60)
                                     GAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCT                                                          <  SRR4302540.2868115/94‑1 (MQ=60)
                                      AAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTA                                                         <  SRR4302540.2773636/94‑1 (MQ=60)
                                      AAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTA                                                         >  SRR4302540.2256984/1‑94 (MQ=60)
                                      AAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTA                                                         >  SRR4302540.4350147/1‑94 (MQ=60)
                                        AGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATT                                                       <  SRR4302540.221422/94‑1 (MQ=60)
                                        AGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATT                                                       <  SRR4302540.4999570/94‑1 (MQ=60)
                                          gactggggaggcggggGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAACCTATGACCGTGTGGTCATTGGTGCTTCTATTCG                                                     <  SRR4302540.5158351/78‑1 (MQ=60)
                                          AACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCG                                                     >  SRR4302540.1041230/1‑94 (MQ=60)
                                          AACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCG                                                     >  SRR4302540.5989868/1‑94 (MQ=60)
                                          AACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCG                                                     <  SRR4302540.3598020/94‑1 (MQ=60)
                                          AACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCG                                                     >  SRR4302540.8178042/1‑94 (MQ=60)
                                          AACTGGGGATCCAGGCGGATGGCGCCCATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCG                                                     <  SRR4302540.3598021/94‑1 (MQ=60)
                                           ACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGC                                                    >  SRR4302540.9145342/1‑94 (MQ=60)
                                            CTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCT                                                   <  SRR4302540.5053399/94‑1 (MQ=60)
                                            CTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCT                                                   <  SRR4302540.1379928/94‑1 (MQ=60)
                                            CTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCT                                                   <  SRR4302540.4361872/94‑1 (MQ=60)
                                            CTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCT                                                   >  SRR4302540.5755425/1‑94 (MQ=60)
                                             TGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTA                                                  >  SRR4302540.8809698/1‑94 (MQ=60)
                                              GGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTAT                                                 <  SRR4302540.10128562/94‑1 (MQ=60)
                                              GGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTAT                                                 <  SRR4302540.8296083/94‑1 (MQ=60)
                                               GGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATG                                                <  SRR4302540.8983814/94‑1 (MQ=60)
                                               GCGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATG                                                <  SRR4302540.108043/94‑1 (MQ=60)
                                                GGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGG                                               <  SRR4302540.2731732/94‑1 (MQ=60)
                                                 GATCCAGGCGGATGTCGCTAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGT                                              <  SRR4302540.4262298/94‑1 (MQ=60)
                                                 GATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGT                                              <  SRR4302540.8459516/94‑1 (MQ=60)
                                                 GATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGT                                              >  SRR4302540.8867761/1‑94 (MQ=60)
                                                 GATCCAGGCGGATGTCGCCAATGAGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGCGTGGTCATTGGTGCTTCTATTCGCTATGGT                                              >  SRR4302540.6961653/1‑94 (MQ=60)
                                                  ATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTC                                             >  SRR4302540.56826/1‑94 (MQ=60)
                                                  ATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTC                                             <  SRR4302540.6369651/94‑1 (MQ=60)
                                                  ATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTC                                             >  SRR4302540.8284373/1‑94 (MQ=60)
                                                    CCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCAC                                           <  SRR4302540.9171656/94‑1 (MQ=60)
                                                    CCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCAC                                           <  SRR4302540.8733545/94‑1 (MQ=60)
                                                    CCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCAC                                           >  SRR4302540.12844/1‑94 (MQ=60)
                                                    CCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCAC                                           <  SRR4302540.9171657/94‑1 (MQ=60)
                                                     CAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACT                                          <  SRR4302540.8098368/94‑1 (MQ=60)
                                                     CAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACT                                          <  SRR4302540.9349075/94‑1 (MQ=60)
                                                     CAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACT                                          <  SRR4302540.5320397/94‑1 (MQ=60)
                                                     CAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACT                                          >  SRR4302540.5626404/1‑94 (MQ=60)
                                                      AGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTA                                         >  SRR4302540.2372914/1‑94 (MQ=60)
                                                       GGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTAC                                        <  SRR4302540.9673324/94‑1 (MQ=60)
                                                         CGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCA                                      <  SRR4302540.853299/94‑1 (MQ=60)
                                                         CGGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCA                                      >  SRR4302540.6979870/1‑94 (MQ=60)
                                                          GGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCAT                                     >  SRR4302540.5267701/1‑94 (MQ=60)
                                                          GGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCAT                                     >  SRR4302540.371844/1‑94 (MQ=60)
                                                          GGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCAT                                     >  SRR4302540.4695104/1‑94 (MQ=60)
                                                          GGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCAT                                     >  SRR4302540.4695105/1‑94 (MQ=60)
                                                          GGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCAT                                     >  SRR4302540.7576890/1‑94 (MQ=60)
                                                          GGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCAT                                     >  SRR4302540.2808034/1‑94 (MQ=60)
                                                          GGATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCAT                                     >  SRR4302540.570997/1‑94 (MQ=60)
                                                           GATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATT                                    <  SRR4302540.4134026/94‑1 (MQ=60)
                                                            ATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTC                                   <  SRR4302540.1690625/94‑1 (MQ=60)
                                                            ATGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTC                                   <  SRR4302540.5750637/94‑1 (MQ=60)
                                                             TGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCA                                  <  SRR4302540.4680704/94‑1 (MQ=60)
                                                             TGTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCA                                  <  SRR4302540.676370/94‑1 (MQ=60)
                                                              GTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAG                                 <  SRR4302540.6705561/94‑1 (MQ=60)
                                                              GTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAG                                 >  SRR4302540.6026693/1‑94 (MQ=60)
                                                              GTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAG                                 >  SRR4302540.5887821/1‑94 (MQ=60)
                                                              GTCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAG                                 >  SRR4302540.1139788/1‑94 (MQ=60)
                                                               TCGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGT                                <  SRR4302540.2821313/94‑1 (MQ=60)
                                                                CGCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTG                               <  SRR4302540.9209873/94‑1 (MQ=60)
                                                                 GCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGT                              <  SRR4302540.7341536/94‑1 (MQ=60)
                                                                 GCCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGT                              >  SRR4302540.1063479/1‑94 (MQ=60)
                                                                  CCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTT                             >  SRR4302540.2394243/1‑94 (MQ=60)
                                                                  CCAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTT                             <  SRR4302540.6694259/94‑1 (MQ=60)
                                                                   CAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTC                            >  SRR4302540.3374792/1‑94 (MQ=60)
                                                                   CAATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTC                            >  SRR4302540.2584968/1‑94 (MQ=60)
                                                                    AATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACGATTCAGTGTTCC                           >  SRR4302540.7273685/1‑94 (MQ=60)
                                                                    AATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCC                           <  SRR4302540.475270/94‑1 (MQ=60)
                                                                     ATGTGCACCGCATTGAAGAGCCACGGTGGGAAAGCTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCA                          <  SRR4302540.4287399/94‑1 (MQ=60)
                                                                     ATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCA                          <  SRR4302540.4287400/94‑1 (MQ=60)
                                                                     ATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCA                          >  SRR4302540.3872923/1‑94 (MQ=60)
                                                                     ATGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCA                          >  SRR4302540.2474471/1‑94 (MQ=60)
                                                                      TGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAG                         <  SRR4302540.8386387/94‑1 (MQ=60)
                                                                      TGTGCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAG                         <  SRR4302540.6613612/94‑1 (MQ=60)
                                                                         GCACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAA                      >  SRR4302540.784584/1‑94 (MQ=60)
                                                                          CACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTTCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAAT                     <  SRR4302540.3160065/94‑1 (MQ=60)
                                                                          CACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAAT                     <  SRR4302540.10081295/94‑1 (MQ=60)
                                                                          CACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAAT                     >  SRR4302540.6648666/1‑94 (MQ=60)
                                                                          CACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAAT                     >  SRR4302540.7831807/1‑94 (MQ=60)
                                                                           ACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATT                    >  SRR4302540.1810426/1‑94 (MQ=60)
                                                                           ACCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATT                    >  SRR4302540.8527216/1‑94 (MQ=60)
                                                                            CCGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTT                   <  SRR4302540.929358/94‑1 (MQ=60)
                                                                             CGCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTG                  <  SRR4302540.1621122/94‑1 (MQ=60)
                                                                              GCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGT                 <  SRR4302540.984102/94‑1 (MQ=60)
                                                                              GCATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGT                 <  SRR4302540.1627730/94‑1 (MQ=60)
                                                                               CATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTC                <  SRR4302540.6312133/94‑1 (MQ=60)
                                                                               CATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTC                >  SRR4302540.3031206/1‑94 (MQ=60)
                                                                                ATTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCA               >  SRR4302540.9713514/1‑94 (MQ=60)
                                                                                 TTGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAA              <  SRR4302540.8619834/94‑1 (MQ=60)
                                                                                  TGAAGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAA             >  SRR4302540.3724916/1‑94 (MQ=60)
                                                                                     AGAACGACGGGGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAA          <  SRR4302540.7931022/94‑1 (MQ=60)
                                                                                     AGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAA          <  SRR4302540.7931018/94‑1 (MQ=60)
                                                                                     AGAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAA          >  SRR4302540.8944538/1‑94 (MQ=60)
                                                                                      GAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAAC         >  SRR4302540.1830832/1‑94 (MQ=60)
                                                                                      GAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAAC         <  SRR4302540.7341957/94‑1 (MQ=60)
                                                                                      GAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAAC         <  SRR4302540.9822379/94‑1 (MQ=60)
                                                                                      GAACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAAA         >  SRR4302540.2120641/1‑94 (MQ=60)
                                                                                       AACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACA        <  SRR4302540.832316/94‑1 (MQ=60)
                                                                                        ACCACGGTGGGAAAACTATTGCCGTGGGGTCATTTGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACAT       <  SRR4302540.3515045/94‑1 (MQ=60)
                                                                                        ACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACAT       >  SRR4302540.5965926/1‑94 (MQ=60)
                                                                                        ACCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACAT       <  SRR4302540.7922397/94‑1 (MQ=60)
                                                                                         CCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATG      <  SRR4302540.669850/94‑1 (MQ=60)
                                                                                         CCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATG      <  SRR4302540.8194502/94‑1 (MQ=60)
                                                                                         CCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATG      <  SRR4302540.7191221/94‑1 (MQ=60)
                                                                                         CCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATG      <  SRR4302540.8648854/94‑1 (MQ=60)
                                                                                         CCACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATG      <  SRR4302540.8743882/94‑1 (MQ=60)
                                                                                          CACGGTGGGAAAACTATGACCGTTTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGC     >  SRR4302540.1817386/1‑94 (MQ=60)
                                                                                          CACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGC     <  SRR4302540.1123487/94‑1 (MQ=60)
                                                                                          CACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGC     <  SRR4302540.7464316/94‑1 (MQ=60)
                                                                                          CACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGC     >  SRR4302540.501241/1‑94 (MQ=60)
                                                                                           ACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCG    >  SRR4302540.319963/1‑94 (MQ=60)
                                                                                           ACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCG    <  SRR4302540.5787799/94‑1 (MQ=60)
                                                                                           ACGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCG    <  SRR4302540.8042871/94‑1 (MQ=60)
                                                                                            CGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTCCCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGA   <  SRR4302540.7255970/94‑1 (MQ=60)
                                                                                            CGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGA   >  SRR4302540.757745/1‑94 (MQ=60)
                                                                                            CGGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGA   <  SRR4302540.9076888/94‑1 (MQ=60)
                                                                                            CGGTGGGAAAACTATGACCGGGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGA   <  SRR4302540.7255969/94‑1 (MQ=60)
                                                                                             GGTGGGAAAACTATGACCGTGTTGTCATTGGTGCTTCTATTCGCTATGGTCACTGCCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGAC  <  SRR4302540.6786069/94‑1 (MQ=60)
                                                                                             GGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGAC  >  SRR4302540.2867148/1‑94 (MQ=60)
                                                                                             GGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGAC  >  SRR4302540.2182510/1‑94 (MQ=60)
                                                                                             GGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGAC  <  SRR4302540.4849421/94‑1 (MQ=60)
                                                                                             GGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGAC  <  SRR4302540.7848732/94‑1 (MQ=60)
                                                                                             GGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGTGTTCCAGGAATTTGTCAAAAAACATGCGAC  <  SRR4302540.7913118/94‑1 (MQ=60)
                                                                                             |                                                                                             
AAACGCGCGAGATTGCCTCCTACCTGGCTTCGGAACTGAAAGAACTGGGGATCCAGGCGGATGTCGCCAATGTGCACCGCATTGAAGAACCACAGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTCAAAAAACATGCGAC  >  NC_000913/4032234‑4032420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑