Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,050,408 A→G S264G (AGT→GGT)  hemN → O2‑independent coproporphyrinogen III oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,050,4080AG99.4% 1489.1 / ‑2.9 470S264G (AGT→GGT) hemNO2‑independent coproporphyrinogen III oxidase
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (252/214);  total (254/216)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 486 total aligned reads displayed.

CTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGT  >  NC_000913/4050318‑4050500
                                                                                          |                                                                                            
  gAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTcc                                                                                          >  2:3568041/1‑93 (MQ=255)
  gAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTcc                                                                                          <  2:4250071/93‑1 (MQ=255)
      cccGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCgcag                                                                                      >  2:1255046/1‑93 (MQ=255)
       ccGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCgcagc                                                                                     >  1:8039232/1‑93 (MQ=255)
       ccGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCgcagc                                                                                     <  1:9540851/93‑1 (MQ=255)
       ccGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCgcagc                                                                                     >  2:8748353/1‑93 (MQ=255)
           tCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCaaaa                                                                                 >  1:7782666/1‑93 (MQ=255)
           tCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCaaaa                                                                                 <  1:2213192/93‑1 (MQ=255)
            cGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCaaaaa                                                                                <  1:4295058/93‑1 (MQ=255)
             gTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAAc                                                                               >  2:9025710/1‑93 (MQ=255)
              tCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACt                                                                              >  2:6801491/1‑93 (MQ=255)
              tCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACt                                                                              <  2:3199718/93‑1 (MQ=255)
              tCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACt                                                                              >  2:529999/1‑93 (MQ=255)
              tCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGCCCGCAGCAAAAACt                                                                              >  2:7118152/1‑93 (MQ=255)
                tGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCg                                                                            >  2:9232211/1‑93 (MQ=255)
                 gattgtCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGa                                                                           <  2:1996128/90‑1 (MQ=255)
                 gAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGa                                                                           >  1:6778129/1‑93 (MQ=255)
                 gAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGa                                                                           >  2:5995361/1‑93 (MQ=255)
                 gAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGa                                                                           <  1:1751250/93‑1 (MQ=255)
                  aGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGat                                                                          >  1:9786959/1‑93 (MQ=255)
                  aGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGat                                                                          <  2:9447653/93‑1 (MQ=255)
                  aGTATCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGat                                                                          >  1:1147382/1‑93 (MQ=255)
                   gtgtCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGata                                                                         <  2:266741/93‑1 (MQ=255)
                   gtgtCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGata                                                                         <  1:2787244/93‑1 (MQ=255)
                   gtgtCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGata                                                                         <  2:8205085/93‑1 (MQ=255)
                    tgtCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGatat                                                                        >  2:1864487/1‑93 (MQ=255)
                    tgtCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGatat                                                                        <  1:8502367/93‑1 (MQ=255)
                    tgtCTTTAACTACGCGCATCTGCAGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTTACCTGCCGGGTCCGCAGCAAAAACCCGatat                                                                        >  2:3497580/1‑93 (MQ=255)
                     gtCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATc                                                                       <  2:9948300/93‑1 (MQ=255)
                      tCTTTAACTCCGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATcc                                                                      >  2:6731623/1‑93 (MQ=255)
                      tCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATcc                                                                      <  2:4783115/93‑1 (MQ=255)
                      gcgttAAATACGCGCATCTGCCGACCATGTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATcc                                                                      <  1:5741328/90‑1 (MQ=255)
                       cTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCt                                                                     <  1:6660352/93‑1 (MQ=255)
                       cTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCt                                                                     >  2:3894360/1‑93 (MQ=255)
                          tAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCa                                                                  >  1:3583725/1‑93 (MQ=255)
                             cTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGa                                                               >  2:3989006/1‑93 (MQ=255)
                              tACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGaa                                                              >  2:6205224/1‑93 (MQ=255)
                                cgcgCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAAc                                                            >  1:443301/1‑93 (MQ=255)
                                 gcgcATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAAcc                                                           >  1:7076525/1‑93 (MQ=255)
                                    cATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATc                                                        <  1:10024447/93‑1 (MQ=255)
                                    cATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATc                                                        >  2:5798722/1‑93 (MQ=255)
                                    cATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATCCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATc                                                        <  2:1212145/93‑1 (MQ=255)
                                      tCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGc                                                      >  1:4879252/1‑93 (MQ=255)
                                      tCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGc                                                      <  1:9249449/93‑1 (MQ=255)
                                      tCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGc                                                      >  1:8631587/1‑93 (MQ=255)
                                        tGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCt                                                    >  1:4744246/1‑93 (MQ=255)
                                          ccGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTc                                                  <  2:1564286/93‑1 (MQ=255)
                                            gACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCt                                                <  2:1418069/93‑1 (MQ=255)
                                             aCCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTg                                               >  1:3091633/1‑93 (MQ=255)
                                              ccATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGa                                              <  2:6502730/93‑1 (MQ=255)
                                              ccATTTTTGCTGCTCAGCGCAAAATCAAAGATCCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGa                                              <  1:5167122/93‑1 (MQ=255)
                                                aTTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACg                                            >  1:7233426/1‑93 (MQ=255)
                                                aTTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACg                                            >  1:6014956/1‑93 (MQ=255)
                                                 tttttGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGc                                           >  1:9895588/1‑93 (MQ=255)
                                                 tttttGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGc                                           >  2:2577462/1‑93 (MQ=255)
                                                    ttGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAAt                                        <  2:2208514/93‑1 (MQ=255)
                                                    ttGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAAt                                        <  1:8733777/93‑1 (MQ=255)
                                                    ttGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAAt                                        <  1:2140595/93‑1 (MQ=255)
                                                     tgctgcTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAAACATCGCCTTCCTGACGCAATc                                       >  2:5679381/1‑93 (MQ=255)
                                                      gctgctCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCg                                      <  1:4973467/93‑1 (MQ=255)
                                                      gctgctCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCg                                      <  2:4408233/93‑1 (MQ=255)
                                                      gctgctCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCg                                      <  1:1887237/93‑1 (MQ=255)
                                                       ctgctCAGCGCAAAATCAAAGATGCTGACCTGCCAGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCgg                                     <  2:6923016/93‑1 (MQ=255)
                                                         gctCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGc                                   >  2:3371436/1‑93 (MQ=255)
                                                         gctCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGc                                   >  1:62422/1‑93 (MQ=255)
                                                         gctCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGc                                   >  1:2374830/1‑93 (MQ=255)
                                                           tCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTa                                 >  2:3685960/1‑93 (MQ=255)
                                                           tCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTa                                 >  2:496385/1‑93 (MQ=255)
                                                             aGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATc                               <  2:4579533/93‑1 (MQ=255)
                                                              gcgcAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCa                              <  1:3290890/93‑1 (MQ=255)
                                                              gcgcAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCa                              >  1:1522081/1‑93 (MQ=255)
                                                                gcAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGt                            >  2:7972268/1‑93 (MQ=255)
                                                                  aaaaTCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGttt                          <  1:644568/93‑1 (MQ=255)
                                                                  aaaaTCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGttt                          >  1:9017346/1‑93 (MQ=255)
                                                                   aaaTCAGAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTACCTGACGCAATCGGGCTATCAGTTTa                         >  2:7318394/1‑93 (MQ=255)
                                                                   aaaTCAAAGATGCTGACCTGCCGGGTCCGCAGCTAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTa                         <  1:9682343/93‑1 (MQ=255)
                                                                   aaaTCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTa                         >  1:2483157/1‑93 (MQ=255)
                                                                   aaaTCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACCCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTa                         <  1:7008057/93‑1 (MQ=255)
                                                                     aTCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATc                       <  2:6659027/93‑1 (MQ=255)
                                                                      tCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCg                      >  1:1635409/1‑93 (MQ=255)
                                                                         aaGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTa                   >  1:3735092/1‑93 (MQ=255)
                                                                          aGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTAt                  >  2:8327624/1‑93 (MQ=255)
                                                                           gATGCTAACCTGCCGGGTCCGCAGCAAAAAATCGATATCCTGCAGGAAACCATCGCCTTCCTGAAGCAATCGGGCTATCAGTTTATCGGTATg                 >  2:5149056/1‑93 (MQ=255)
                                                                             tGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGa               <  2:1017111/93‑1 (MQ=255)
                                                                              gCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGAt              <  1:7527512/93‑1 (MQ=255)
                                                                              gCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGAt              <  1:5861428/93‑1 (MQ=255)
                                                                                 gACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCAc           >  2:2747475/1‑93 (MQ=255)
                                                                                 gACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCAc           >  2:968631/1‑93 (MQ=255)
                                                                                  aCCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGACACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACt          <  2:6324399/93‑1 (MQ=255)
                                                                                  aCCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACt          >  1:5476181/1‑93 (MQ=255)
                                                                                  aCCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACt          <  2:8471586/93‑1 (MQ=255)
                                                                                  aCCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACt          >  1:8354448/1‑93 (MQ=255)
                                                                                    cTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACttt        <  1:9121047/93‑1 (MQ=255)
                                                                                     tGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTg       >  2:3002557/1‑93 (MQ=255)
                                                                                      gCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGc      <  1:3874616/93‑1 (MQ=255)
                                                                                      gCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGc      >  1:4138608/1‑93 (MQ=255)
                                                                                      gCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGc      <  1:7352637/93‑1 (MQ=255)
                                                                                       ccGGGTCCGCAGCAAAACCTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGcc     <  2:7622849/93‑1 (MQ=255)
                                                                                       ccGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGcc     >  2:7781813/1‑93 (MQ=255)
                                                                                          aGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGt  >  2:8522168/1‑93 (MQ=255)
                                                                                          |                                                                                            
CTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGT  >  NC_000913/4050318‑4050500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 253 total aligned reads displayed.
GAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTC  >  NC_000913/4050315‑4050501
                                                                                             |                                                                                             
GAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGG                                                                                               <  SRR4302540.7906014/94‑1 (MQ=60)
    TGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCC                                                                                           >  SRR4302540.5906667/1‑94 (MQ=60)
    TGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCC                                                                                           <  SRR4302540.7568090/94‑1 (MQ=60)
    TGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCC                                                                                           <  SRR4302540.8387626/94‑1 (MQ=60)
    TGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCC                                                                                           <  SRR4302540.9839335/94‑1 (MQ=60)
      AACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGC                                                                                         >  SRR4302540.1037443/1‑94 (MQ=60)
       ACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTTCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCA                                                                                        >  SRR4302540.9849128/1‑94 (MQ=60)
        CCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAG                                                                                       <  SRR4302540.1916575/94‑1 (MQ=60)
        CCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAG                                                                                       <  SRR4302540.972159/94‑1 (MQ=60)
        CCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAG                                                                                       >  SRR4302540.3402687/1‑94 (MQ=60)
         CCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGC                                                                                      >  SRR4302540.635375/1‑94 (MQ=60)
         CCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGC                                                                                      >  SRR4302540.8132657/1‑94 (MQ=60)
          CCTATCGTCTGAGTGTCTTTAACGACGCGCATCTGCCGACCAGTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCA                                                                                     <  SRR4302540.9014464/94‑1 (MQ=60)
          CCGATCGTCTGGGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCA                                                                                     <  SRR4302540.7606801/94‑1 (MQ=60)
          CCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCA                                                                                     <  SRR4302540.9653753/94‑1 (MQ=60)
          CCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCA                                                                                     <  SRR4302540.1875570/94‑1 (MQ=60)
          CCGATCGTCTGACTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCA                                                                                     <  SRR4302540.9014465/94‑1 (MQ=60)
           CGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAA                                                                                    <  SRR4302540.244179/94‑1 (MQ=60)
           CGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAA                                                                                    >  SRR4302540.9412969/1‑94 (MQ=60)
           CGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAA                                                                                    >  SRR4302540.2442207/1‑94 (MQ=60)
           CGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAACCAAAGATGATAACCTGCCGGGTCCGCAGCAA                                                                                    >  SRR4302540.7883224/1‑94 (MQ=60)
            GATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAA                                                                                   <  SRR4302540.3589544/94‑1 (MQ=60)
              TCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAA                                                                                 <  SRR4302540.2237084/94‑1 (MQ=60)
              TCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAA                                                                                 >  SRR4302540.4227109/1‑94 (MQ=60)
              TCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAA                                                                                 >  SRR4302540.7264701/1‑94 (MQ=60)
               CGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAAC                                                                                <  SRR4302540.4341789/94‑1 (MQ=60)
               CGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAAC                                                                                >  SRR4302540.9307620/1‑94 (MQ=60)
               CGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAAC                                                                                <  SRR4302540.780747/94‑1 (MQ=60)
                GTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGGCGCAAAAACT                                                                               >  SRR4302540.2156449/1‑94 (MQ=60)
                GTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACT                                                                               >  SRR4302540.7413335/1‑94 (MQ=60)
                GTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACT                                                                               >  SRR4302540.6056852/1‑94 (MQ=60)
                GTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACT                                                                               >  SRR4302540.7145131/1‑94 (MQ=60)
                GTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACT                                                                               >  SRR4302540.4004001/1‑94 (MQ=60)
                GTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACT                                                                               >  SRR4302540.7032671/1‑94 (MQ=60)
                 TCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTC                                                                              <  SRR4302540.3679976/94‑1 (MQ=60)
                  CTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCACAAACTCG                                                                             >  SRR4302540.9776256/1‑94 (MQ=60)
                  CTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCG                                                                             >  SRR4302540.9776255/1‑94 (MQ=60)
                  CTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCG                                                                             >  SRR4302540.5972921/1‑94 (MQ=60)
                   TGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGA                                                                            >  SRR4302540.6853052/1‑94 (MQ=60)
                   TGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGA                                                                            >  SRR4302540.9283905/1‑94 (MQ=60)
                   TGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGA                                                                            >  SRR4302540.5617769/1‑94 (MQ=60)
                    GAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGAT                                                                           >  SRR4302540.9901628/1‑94 (MQ=60)
                    GAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGAT                                                                           <  SRR4302540.1770223/94‑1 (MQ=60)
                    GAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGAT                                                                           >  SRR4302540.3207755/1‑94 (MQ=60)
                    GAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGAT                                                                           >  SRR4302540.3822366/1‑94 (MQ=60)
                    GAGTATCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGAT                                                                           >  SRR4302540.1159353/1‑94 (MQ=60)
                     AGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATA                                                                          >  SRR4302540.8734707/1‑94 (MQ=60)
                      GTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATAT                                                                         >  SRR4302540.2742666/1‑94 (MQ=60)
                      GTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATAT                                                                         <  SRR4302540.2817346/94‑1 (MQ=60)
                       TGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATC                                                                        >  SRR4302540.2531233/1‑94 (MQ=60)
                       TGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATC                                                                        <  SRR4302540.10062536/94‑1 (MQ=60)
                       TGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATC                                                                        <  SRR4302540.8601167/94‑1 (MQ=60)
                        GTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCC                                                                       >  SRR4302540.8808083/1‑94 (MQ=60)
                        GTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCC                                                                       <  SRR4302540.8458822/94‑1 (MQ=60)
                         ttctcAACTACGCCCATCGGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCT                                                                      <  SRR4302540.5804616/89‑1 (MQ=60)
                          CTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTG                                                                     <  SRR4302540.6803790/94‑1 (MQ=60)
                          CTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTG                                                                     <  SRR4302540.6733993/94‑1 (MQ=60)
                           TTTAACTACGCGCATCTGCCGACCATTTTTGCTTCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGC                                                                    <  SRR4302540.2298548/94‑1 (MQ=60)
                           TTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGC                                                                    >  SRR4302540.849680/1‑94 (MQ=60)
                            TTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCA                                                                   >  SRR4302540.6062565/1‑94 (MQ=60)
                            TTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCA                                                                   >  SRR4302540.3622870/1‑94 (MQ=60)
                            TTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCA                                                                   <  SRR4302540.8203893/94‑1 (MQ=60)
                             TAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAG                                                                  <  SRR4302540.913233/94‑1 (MQ=60)
                              AACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGG                                                                 >  SRR4302540.8838686/1‑94 (MQ=60)
                               ACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGA                                                                <  SRR4302540.3638215/94‑1 (MQ=60)
                                CTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAA                                                               >  SRR4302540.3831846/1‑94 (MQ=60)
                                CTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAA                                                               <  SRR4302540.1605062/94‑1 (MQ=60)
                                 TACGCGCATCTGCCGCCCAGTGTTGCGGCTCAGCGCAAAAGCAAGGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAA                                                              <  SRR4302540.4701045/94‑1 (MQ=60)
                                 TACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAA                                                              >  SRR4302540.9958684/1‑94 (MQ=60)
                                 TACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAA                                                              <  SRR4302540.4701044/94‑1 (MQ=60)
                                 TACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAA                                                              <  SRR4302540.6693324/94‑1 (MQ=60)
                                  TCGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAAC                                                             <  SRR4302540.4255589/94‑1 (MQ=60)
                                  ACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAAC                                                             >  SRR4302540.288394/1‑94 (MQ=60)
                                  ACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAAC                                                             >  SRR4302540.448026/1‑94 (MQ=60)
                                   CGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACC                                                            >  SRR4302540.7155271/1‑94 (MQ=60)
                                     CGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCAT                                                          <  SRR4302540.7760557/94‑1 (MQ=60)
                                      GCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATC                                                         <  SRR4302540.7958099/94‑1 (MQ=60)
                                      GCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATC                                                         <  SRR4302540.446433/94‑1 (MQ=60)
                                       CATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCG                                                        <  SRR4302540.758340/94‑1 (MQ=60)
                                       CATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCG                                                        >  SRR4302540.1513293/1‑94 (MQ=60)
                                        ATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGC                                                       >  SRR4302540.4931640/1‑94 (MQ=60)
                                        ATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGC                                                       >  SRR4302540.5222568/1‑94 (MQ=60)
                                        ATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGC                                                       >  SRR4302540.297802/1‑94 (MQ=60)
                                        ATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGC                                                       >  SRR4302540.8731851/1‑94 (MQ=60)
                                         TCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCC                                                      <  SRR4302540.9360182/94‑1 (MQ=60)
                                         TCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCC                                                      >  SRR4302540.250965/1‑94 (MQ=60)
                                         TCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCC                                                      <  SRR4302540.6645959/94‑1 (MQ=60)
                                          CTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCT                                                     >  SRR4302540.4794874/1‑94 (MQ=60)
                                           TGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTT                                                    <  SRR4302540.488100/94‑1 (MQ=60)
                                            GCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTC                                                   <  SRR4302540.1747845/94‑1 (MQ=60)
                                             CCGCCCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCC                                                  <  SRR4302540.1242293/94‑1 (MQ=60)
                                             CCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCC                                                  >  SRR4302540.6130861/1‑94 (MQ=60)
                                             CCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCC                                                  >  SRR4302540.6865377/1‑94 (MQ=60)
                                             CCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCC                                                  <  SRR4302540.9481311/94‑1 (MQ=60)
                                              CGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCT                                                 >  SRR4302540.6386955/1‑94 (MQ=60)
                                              CGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCT                                                 >  SRR4302540.354782/1‑94 (MQ=60)
                                              CGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCT                                                 >  SRR4302540.8252299/1‑94 (MQ=60)
                                               GACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTG                                                >  SRR4302540.4845458/1‑94 (MQ=60)
                                               GACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTG                                                >  SRR4302540.3125544/1‑94 (MQ=60)
                                                ACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGA                                               >  SRR4302540.1694547/1‑94 (MQ=60)
                                                 CCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGAC                                              <  SRR4302540.2088357/94‑1 (MQ=60)
                                                 CCATTTTTGCTGCTCAGCGCAAAATCAAAGATCCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGAC                                              <  SRR4302540.5223324/94‑1 (MQ=60)
                                                  CATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCTGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACG                                             >  SRR4302540.2664483/1‑94 (MQ=60)
                                                  CATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACG                                             >  SRR4302540.6081660/1‑94 (MQ=60)
                                                  CATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACG                                             >  SRR4302540.7314103/1‑94 (MQ=60)
                                                   ATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTNCANNAAANCATCGCCNNCNTNANGC                                            >  SRR4302540.124972/1‑94 (MQ=60)
                                                   ATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGC                                            >  SRR4302540.6510165/1‑94 (MQ=60)
                                                   ATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGC                                            >  SRR4302540.10011149/1‑94 (MQ=60)
                                                       TTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATC                                        <  SRR4302540.8835192/94‑1 (MQ=60)
                                                       TTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATC                                        <  SRR4302540.4502020/94‑1 (MQ=60)
                                                       TTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATC                                        <  SRR4302540.2163600/94‑1 (MQ=60)
                                                       TTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATC                                        <  SRR4302540.7935650/94‑1 (MQ=60)
                                                       TTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATC                                        <  SRR4302540.7140232/94‑1 (MQ=60)
                                                        TGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCG                                       <  SRR4302540.7427215/94‑1 (MQ=60)
                                                        TGCCGCTCAGCGCAAAATCAAAGATCGTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCG                                       <  SRR4302540.4808349/94‑1 (MQ=60)
                                                         TCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGG                                      <  SRR4302540.2182808/94‑1 (MQ=60)
                                                         GCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGG                                      <  SRR4302540.1576069/94‑1 (MQ=60)
                                                         GCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGG                                      <  SRR4302540.5027288/94‑1 (MQ=60)
                                                         GCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGG                                      <  SRR4302540.1907898/94‑1 (MQ=60)
                                                          CTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGG                                     >  SRR4302540.9945498/1‑94 (MQ=60)
                                                           TGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAACAACTCGATATCCTGCTGGAAACCATCGCCTTCCTGACGCCATCGGGT                                    >  SRR4302540.2400418/1‑94 (MQ=60)
                                                           TGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGCAACCATCGCCTTCCTGACGCAATCGGCC                                    >  SRR4302540.5745072/1‑94 (MQ=60)
                                                           TGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGC                                    >  SRR4302540.2400419/1‑94 (MQ=60)
                                                           TGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGC                                    <  SRR4302540.9385173/94‑1 (MQ=60)
                                                           TGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGC                                    <  SRR4302540.1132898/94‑1 (MQ=60)
                                                           TGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGC                                    >  SRR4302540.63051/1‑94 (MQ=60)
                                                            GCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCT                                   >  SRR4302540.6835149/1‑94 (MQ=60)
                                                              TCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTAT                                 >  SRR4302540.9864476/1‑94 (MQ=60)
                                                              TCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTAT                                 <  SRR4302540.5279126/94‑1 (MQ=60)
                                                               CAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATC                                <  SRR4302540.8633036/94‑1 (MQ=60)
                                                               CAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATC                                <  SRR4302540.3294862/94‑1 (MQ=60)
                                                               CAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATC                                >  SRR4302540.8809368/1‑94 (MQ=60)
                                                                AGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCA                               >  SRR4302540.1538927/1‑94 (MQ=60)
                                                                AGCGCAAAATCAAAGATGCTGACCTGCCGGGGCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCA                               >  SRR4302540.6847616/1‑94 (MQ=60)
                                                                 accCAAAAGCAAAGATGATGACCTGCCGGGTCCGGAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAG                              <  SRR4302540.5808102/91‑1 (MQ=60)
                                                                 GCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAG                              <  SRR4302540.3326893/94‑1 (MQ=60)
                                                                 GCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAG                              <  SRR4302540.5808101/94‑1 (MQ=60)
                                                                   GCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTT                            <  SRR4302540.1106752/94‑1 (MQ=60)
                                                                    CAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTT                           >  SRR4302540.9446112/1‑94 (MQ=60)
                                                                    CAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTT                           >  SRR4302540.9125546/1‑94 (MQ=60)
                                                                     AAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTA                          >  SRR4302540.8588530/1‑94 (MQ=60)
                                                                     AAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTA                          >  SRR4302540.1385086/1‑94 (MQ=60)
                                                                     AAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTA                          <  SRR4302540.651437/94‑1 (MQ=60)
                                                                     AAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTA                          >  SRR4302540.5024191/1‑94 (MQ=60)
                                                                      AAATCAAAGATGCTGACCTGCCGGGTCCGCAGCTAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTAT                         <  SRR4302540.9796260/94‑1 (MQ=60)
                                                                      AAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGACACCATCCCCTTCCTGACGCAATAGGGCTAGCAGTTTAT                         >  SRR4302540.9214766/1‑94 (MQ=60)
                                                                      AAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTAT                         >  SRR4302540.5577612/1‑94 (MQ=60)
                                                                      AAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACCCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTAT                         <  SRR4302540.7085847/94‑1 (MQ=60)
                                                                      AAATCAAAGATGCTGACCTGCCGGGTCAGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTAT                         <  SRR4302540.40831/94‑1 (MQ=60)
                                                                        ttctcagcTGCTGCCCTGCCGGGTCCGCCGCAAAACCTCGATCTCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCG                       <  SRR4302540.7792591/86‑1 (MQ=60)
                                                                        ATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCG                       <  SRR4302540.4995765/94‑1 (MQ=60)
                                                                        ATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCG                       >  SRR4302540.1653299/1‑94 (MQ=60)
                                                                        ATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCG                       <  SRR4302540.4995767/94‑1 (MQ=60)
                                                                         TCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGG                      >  SRR4302540.4480880/1‑94 (MQ=60)
                                                                          CAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGT                     >  SRR4302540.5388660/1‑94 (MQ=60)
                                                                          CAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGT                     <  SRR4302540.1901836/94‑1 (MQ=60)
                                                                          CAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGT                     >  SRR4302540.6189450/1‑94 (MQ=60)
                                                                          CAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGT                     >  SRR4302540.7402363/1‑94 (MQ=60)
                                                                          CAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGT                     >  SRR4302540.8577200/1‑94 (MQ=60)
                                                                           AAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTA                    >  SRR4302540.7030275/1‑94 (MQ=60)
                                                                           AAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTA                    >  SRR4302540.2208893/1‑94 (MQ=60)
                                                                           AAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTA                    >  SRR4302540.10063532/1‑94 (MQ=60)
                                                                           AAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTA                    >  SRR4302540.3775845/1‑94 (MQ=60)
                                                                            tcgagtttttCCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTAT                   <  SRR4302540.1369108/84‑1 (MQ=60)
                                                                            AAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTAT                   <  SRR4302540.10011776/94‑1 (MQ=60)
                                                                              GATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGG                 >  SRR4302540.5533055/1‑94 (MQ=60)
                                                                                TGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGAT               <  SRR4302540.1658227/94‑1 (MQ=60)
                                                                                 GCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATC              <  SRR4302540.7611759/94‑1 (MQ=60)
                                                                                 GCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATC              <  SRR4302540.7257159/94‑1 (MQ=60)
                                                                                 GCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATC              <  SRR4302540.5926244/94‑1 (MQ=60)
                                                                                   TGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCAC            >  SRR4302540.7150551/1‑94 (MQ=60)
                                                                                   TGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCAC            <  SRR4302540.2167098/94‑1 (MQ=60)
                                                                                    GACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACT           >  SRR4302540.8451413/1‑94 (MQ=60)
                                                                                    GACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACT           >  SRR4302540.3941115/1‑94 (MQ=60)
                                                                                    GACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACT           >  SRR4302540.3118129/1‑94 (MQ=60)
                                                                                    GACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACT           >  SRR4302540.5536454/1‑94 (MQ=60)
                                                                                     ACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTT          >  SRR4302540.5109936/1‑94 (MQ=60)
                                                                                      CCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTT         <  SRR4302540.178158/94‑1 (MQ=60)
                                                                                      CCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTT         >  SRR4302540.2415915/1‑94 (MQ=60)
                                                                                      CCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTT         <  SRR4302540.3223073/94‑1 (MQ=60)
                                                                                       CTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTG        >  SRR4302540.5565198/1‑94 (MQ=60)
                                                                                       CTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTG        >  SRR4302540.7228541/1‑94 (MQ=60)
                                                                                       CTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTG        <  SRR4302540.9230681/94‑1 (MQ=60)
                                                                                       CTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTG        >  SRR4302540.1550737/1‑94 (MQ=60)
                                                                                       CTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTG        <  SRR4302540.9348356/94‑1 (MQ=60)
                                                                                       CTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTC        >  SRR4302540.8714490/1‑94 (MQ=60)
                                                                                        TGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGC       >  SRR4302540.997048/1‑94 (MQ=60)
                                                                                        TGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGC       >  SRR4302540.3740658/1‑94 (MQ=60)
                                                                                        TGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGC       >  SRR4302540.4183388/1‑94 (MQ=60)
                                                                                        CGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGC       <  SRR4302540.6227476/94‑1 (MQ=60)
                                                                                         GCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCC      <  SRR4302540.3916728/94‑1 (MQ=60)
                                                                                         GCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCC      >  SRR4302540.4277108/1‑94 (MQ=60)
                                                                                         GCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCC      <  SRR4302540.7434647/94‑1 (MQ=60)
                                                                                          CCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCC     <  SRR4302540.4371669/94‑1 (MQ=60)
                                                                                           CGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCG    <  SRR4302540.3296081/94‑1 (MQ=60)
                                                                                           CGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCG    <  SRR4302540.2441150/94‑1 (MQ=60)
                                                                                           CGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCG    >  SRR4302540.6722656/1‑94 (MQ=60)
                                                                                           CGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCG    <  SRR4302540.680383/94‑1 (MQ=60)
                                                                                           CGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCG    <  SRR4302540.807220/94‑1 (MQ=60)
                                                                                            GGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGT   >  SRR4302540.3210481/1‑94 (MQ=60)
                                                                                             |                                                                                             
GAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTC  >  NC_000913/4050315‑4050501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑