Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,050,408 A→G S264G (AGT→GGT)  hemN → O2‑independent coproporphyrinogen III oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,050,4080AG100.0% 826.6 / NA 258S264G (AGT→GGT) hemNO2‑independent coproporphyrinogen III oxidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (134/124);  total (134/124)

Only 100 of 266 total aligned reads displayed.

CTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCC  >  NC_000913/4050318‑4050497
                                                                                          |                                                                                         
cTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGt                                                                                         <  2:2558053/93‑1 (MQ=255)
 tGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTc                                                                                        >  1:4139017/1‑93 (MQ=255)
  gAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTcc                                                                                       <  2:4036340/93‑1 (MQ=255)
     ccccGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCgca                                                                                    >  1:4564040/1‑93 (MQ=255)
     ccccGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCgca                                                                                    <  2:3615100/93‑1 (MQ=255)
      cccGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCgcag                                                                                   <  2:2221253/93‑1 (MQ=255)
      cccGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCgcag                                                                                   >  1:3937363/1‑93 (MQ=255)
      cccGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCgcag                                                                                   >  1:4811660/1‑93 (MQ=255)
       ccGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCgcagc                                                                                  >  2:626753/1‑93 (MQ=255)
        cGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCgcagca                                                                                 >  2:2853605/1‑93 (MQ=255)
          aTCGTCTGAGCGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCaaa                                                                               >  1:4911269/1‑93 (MQ=255)
           tCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCaaaa                                                                              >  2:2470423/1‑93 (MQ=255)
           tCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCaaaa                                                                              >  1:368992/1‑93 (MQ=255)
             gTCTGAGTGTCTTTAACTACGCGCATCTGCCGCCCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCACAAAc                                                                            >  1:583702/1‑93 (MQ=255)
              tCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACt                                                                           >  1:1002509/1‑93 (MQ=255)
                tGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCg                                                                         >  1:6216475/1‑93 (MQ=255)
                tGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCg                                                                         >  1:705742/1‑93 (MQ=255)
                tGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCg                                                                         >  2:5314455/1‑93 (MQ=255)
                  aGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGat                                                                       >  1:5464700/1‑93 (MQ=255)
                   gtgtCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGata                                                                      <  1:2302823/93‑1 (MQ=255)
                    tgtCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGatat                                                                     >  2:5576779/1‑93 (MQ=255)
                    tgtCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGatat                                                                     <  2:2151043/93‑1 (MQ=255)
                      tCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATcc                                                                   >  1:2777703/1‑93 (MQ=255)
                      tCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATcc                                                                   <  1:1533186/93‑1 (MQ=255)
                       cTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCt                                                                  >  2:3129103/1‑93 (MQ=255)
                       cTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCt                                                                  <  2:523684/93‑1 (MQ=255)
                       cTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCt                                                                  >  2:1705187/1‑93 (MQ=255)
                        tttAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTg                                                                 >  1:1094248/1‑93 (MQ=255)
                        tttAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATCCTGACCTGCCGGGTCCGCAGCAAAAGCTCGATATCCTg                                                                 <  2:4367504/93‑1 (MQ=255)
                         atAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGc                                                                <  2:762383/92‑1 (MQ=255)
                          tAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCa                                                               >  1:2970821/1‑93 (MQ=255)
                           aaCTCCGCGCATCTGCCGACCATGTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAg                                                              <  1:5191746/93‑1 (MQ=255)
                            aCTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAgg                                                             <  2:3011820/93‑1 (MQ=255)
                             cTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGa                                                            >  2:687938/1‑93 (MQ=255)
                              tACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGaa                                                           >  1:1311882/1‑93 (MQ=255)
                              tACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGaa                                                           <  1:1849465/93‑1 (MQ=255)
                               aCGCGCATCTGCCGCCCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGaaa                                                          <  2:2963363/93‑1 (MQ=255)
                               aCGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGaaa                                                          >  1:2005398/1‑93 (MQ=255)
                                 gcgcATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGACAcc                                                        >  2:3658583/1‑93 (MQ=255)
                                   gcATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCAt                                                      >  1:1977923/1‑93 (MQ=255)
                                    cATCTGCCGGCTATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATc                                                     <  2:6086572/93‑1 (MQ=255)
                                     aTCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCg                                                    <  2:865626/93‑1 (MQ=255)
                                      tCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGc                                                   >  2:5079381/1‑93 (MQ=255)
                                      tCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGc                                                   <  1:5241871/93‑1 (MQ=255)
                                       cTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGcc                                                  <  2:5982756/93‑1 (MQ=255)
                                        tGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCt                                                 <  2:5499260/93‑1 (MQ=255)
                                        tGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCt                                                 >  2:1504696/1‑93 (MQ=255)
                                         gCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCtt                                                <  1:4239602/93‑1 (MQ=255)
                                          ccGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTc                                               <  1:6017541/93‑1 (MQ=255)
                                           cGAGCATGTTTGCGGCTCAGCCGAAAACCATAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTcc                                              <  2:4180358/93‑1 (MQ=255)
                                           cGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTcc                                              >  2:4779959/1‑93 (MQ=255)
                                           cGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTcc                                              <  2:176200/93‑1 (MQ=255)
                                            gACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCt                                             >  1:4781970/1‑93 (MQ=255)
                                             aCCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTg                                            >  2:896009/1‑93 (MQ=255)
                                             aCCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTg                                            >  2:946592/1‑93 (MQ=255)
                                             aCCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTg                                            >  1:5113537/1‑93 (MQ=255)
                                               cATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGAc                                          >  2:1339584/1‑93 (MQ=255)
                                                  ttttGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCa                                       <  1:4653991/93‑1 (MQ=255)
                                                   tttGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCaa                                      <  2:5939029/93‑1 (MQ=255)
                                                     tgctgcTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATc                                    >  2:3827026/1‑93 (MQ=255)
                                                         gctCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGc                                <  2:2691360/93‑1 (MQ=255)
                                                         gctCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGc                                >  2:1664203/1‑93 (MQ=255)
                                                           tCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTa                              >  2:2616323/1‑93 (MQ=255)
                                                            cAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTAt                             >  1:2487072/1‑93 (MQ=255)
                                                             aGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATc                            <  1:4340052/93‑1 (MQ=255)
                                                             aGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATc                            <  1:4465749/93‑1 (MQ=255)
                                                             aGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATc                            >  2:4010375/1‑93 (MQ=255)
                                                                gcAAAATCAAAGATTCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGt                         <  1:2672911/93‑1 (MQ=255)
                                                                gcAAAATCAAAGATGCTGGCCTGCCGGGTCCGCAGCAAAAAATCGATATACTGCAGGAAACCATCGCCTTCCTGACGCAATAGGGCTATCCGt                         >  2:1085795/1‑93 (MQ=255)
                                                                   aaaTCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTa                      >  1:1762749/1‑93 (MQ=255)
                                                                   aaaTCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTa                      <  1:3167707/93‑1 (MQ=255)
                                                                    aaTCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTAt                     <  2:6161818/93‑1 (MQ=255)
                                                                    aaTCAAAGATGCGGACCTGCCGGGTCCGCAACAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTAt                     <  1:741998/93‑1 (MQ=255)
                                                                     aTCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATc                    >  2:4728358/1‑93 (MQ=255)
                                                                      tCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCg                   >  1:5608424/1‑93 (MQ=255)
                                                                      tCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCg                   <  1:4047257/93‑1 (MQ=255)
                                                                      tCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCg                   >  2:1992563/1‑93 (MQ=255)
                                                                       cAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCgg                  <  2:3367297/93‑1 (MQ=255)
                                                                        aaaGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGt                 >  1:5510176/1‑93 (MQ=255)
                                                                         aaGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTa                <  1:2470423/93‑1 (MQ=255)
                                                                         aaGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTa                >  2:2254169/1‑93 (MQ=255)
                                                                          aGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTAt               <  1:3335165/93‑1 (MQ=255)
                                                                           gATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATg              >  1:1376882/1‑93 (MQ=255)
                                                                              gCTGACCTGCCGGGTCCGCAGCAAAAACTCGATACCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGAt           <  2:2048147/93‑1 (MQ=255)
                                                                               cTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATc          <  2:24326/93‑1 (MQ=255)
                                                                               cTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATc          <  2:2319713/93‑1 (MQ=255)
                                                                                 gACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCAc        >  1:4996842/1‑93 (MQ=255)
                                                                                 gACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCAc        >  2:3897655/1‑93 (MQ=255)
                                                                                 gACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCAc        >  2:1049946/1‑93 (MQ=255)
                                                                                 gACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCAc        <  2:1295493/93‑1 (MQ=255)
                                                                                 gACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCAc        >  1:2176647/1‑93 (MQ=255)
                                                                                  aCCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCTCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACt       <  1:4546723/93‑1 (MQ=255)
                                                                                  aCCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACt       <  2:4946441/93‑1 (MQ=255)
                                                                                   ccTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACtt      <  2:4518677/93‑1 (MQ=255)
                                                                                   ccTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACtt      <  2:4451868/93‑1 (MQ=255)
                                                                                    cTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACttt     <  1:4855585/93‑1 (MQ=255)
                                                                                    cTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACttt     >  1:837126/1‑93 (MQ=255)
                                                                                     tGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTg    >  2:4559237/1‑93 (MQ=255)
                                                                                      gCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGc   >  1:4753460/1‑93 (MQ=255)
                                                                                       ccGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGcc  >  1:2717923/1‑93 (MQ=255)
                                                                                          |                                                                                         
CTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCC  >  NC_000913/4050318‑4050497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTC  >  NC_000913/4050316‑4050501
                                                                                            |                                                                                             
AGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGG                                                                                              >  SRR4302533.4916824/1‑94 (MQ=60)
AGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGG                                                                                              <  SRR4302533.4363168/94‑1 (MQ=60)
  CTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTC                                                                                            >  SRR4302533.4164125/1‑94 (MQ=60)
  CTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTC                                                                                            >  SRR4302533.4164123/1‑94 (MQ=60)
   TGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCC                                                                                           <  SRR4302533.4768263/94‑1 (MQ=60)
      ACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCA                                                                                        >  SRR4302533.4591714/1‑94 (MQ=60)
       CCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAG                                                                                       >  SRR4302533.4840822/1‑94 (MQ=60)
       CCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAG                                                                                       >  SRR4302533.3961398/1‑94 (MQ=60)
         CCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCA                                                                                     <  SRR4302533.4126694/94‑1 (MQ=60)
           GATCGTCTGAGCGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAA                                                                                   >  SRR4302533.4941021/1‑94 (MQ=60)
            ATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAA                                                                                  >  SRR4302533.371220/1‑94 (MQ=60)
              CGTCTGAGTGTCTTTAACTACGCGCATCTGCCGCCCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCACAAAC                                                                                >  SRR4302533.587144/1‑94 (MQ=60)
               GTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACT                                                                               >  SRR4302533.1008362/1‑94 (MQ=60)
               GTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACT                                                                               >  SRR4302533.5634403/1‑94 (MQ=60)
                TCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTC                                                                              >  SRR4302533.2707856/1‑94 (MQ=60)
                TCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTC                                                                              <  SRR4302533.2773483/94‑1 (MQ=60)
                TCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTC                                                                              >  SRR4302533.5405353/1‑94 (MQ=60)
                TCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTC                                                                              >  SRR4302533.4924231/1‑94 (MQ=60)
                 CTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCG                                                                             >  SRR4302533.4823703/1‑94 (MQ=60)
                 CTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCG                                                                             >  SRR4302533.1800846/1‑94 (MQ=60)
                 CTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCG                                                                             >  SRR4302533.6253752/1‑94 (MQ=60)
                 CTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCG                                                                             >  SRR4302533.709909/1‑94 (MQ=60)
                 CTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGCTGCGCAGCAAAAACTCG                                                                             >  SRR4302533.5056701/1‑94 (MQ=60)
                   GAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGAT                                                                           <  SRR4302533.5177703/94‑1 (MQ=60)
                   GAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGAT                                                                           >  SRR4302533.5497630/1‑94 (MQ=60)
                    AGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATA                                                                          <  SRR4302533.2502070/94‑1 (MQ=60)
                     GTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATAT                                                                         <  SRR4302533.2317089/94‑1 (MQ=60)
                      TGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATC                                                                        <  SRR4302533.719731/94‑1 (MQ=60)
                       GTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCC                                                                       >  SRR4302533.2794766/1‑94 (MQ=60)
                        TCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCT                                                                      <  SRR4302533.1542396/94‑1 (MQ=60)
                         CTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTG                                                                     >  SRR4302533.1100724/1‑94 (MQ=60)
                         CTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTG                                                                     >  SRR4302533.2850130/1‑94 (MQ=60)
                         CTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTG                                                                     >  SRR4302533.1170721/1‑94 (MQ=60)
                           TTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCA                                                                   >  SRR4302533.2988988/1‑94 (MQ=60)
                             AACTCCGCGCATCTGCCGACCATGTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGG                                                                 <  SRR4302533.5223065/94‑1 (MQ=60)
                             AACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGG                                                                 <  SRR4302533.3127189/94‑1 (MQ=60)
                              ACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATTCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGA                                                                <  SRR4302533.5242853/94‑1 (MQ=60)
                              ACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGA                                                                >  SRR4302533.1053489/1‑94 (MQ=60)
                               CTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAA                                                               >  SRR4302533.1319755/1‑94 (MQ=60)
                                TACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAA                                                              <  SRR4302533.1860747/94‑1 (MQ=60)
                                TACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAA                                                              >  SRR4302533.2017748/1‑94 (MQ=60)
                                    CGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCAT                                                          >  SRR4302533.5237041/1‑94 (MQ=60)
                                    CGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCAT                                                          >  SRR4302533.1990035/1‑94 (MQ=60)
                                     GCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGCCCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATC                                                         <  SRR4302533.2420267/94‑1 (MQ=60)
                                     GCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATC                                                         <  SRR4302533.1990651/94‑1 (MQ=60)
                                     GCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATC                                                         <  SRR4302533.201288/94‑1 (MQ=60)
                                      CATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCG                                                        <  SRR4302533.774096/94‑1 (MQ=60)
                                       ATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAAGCATCGC                                                       >  SRR4302533.5348811/1‑94 (MQ=60)
                                        TCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCC                                                      <  SRR4302533.2592514/94‑1 (MQ=60)
                                        TCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCC                                                      <  SRR4302533.5273380/94‑1 (MQ=60)
                                          TGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTT                                                    <  SRR4302533.1536299/94‑1 (MQ=60)
                                           GCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTC                                                   <  SRR4302533.4265207/94‑1 (MQ=60)
                                            CCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCC                                                  <  SRR4302533.6054003/94‑1 (MQ=60)
                                             CGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATTCTGCAGGAAACCATCGCCTTCCT                                                 >  SRR4302533.894390/1‑94 (MQ=60)
                                             CGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCT                                                 <  SRR4302533.3196037/94‑1 (MQ=60)
                                             CGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCT                                                 >  SRR4302533.4810944/1‑94 (MQ=60)
                                             CGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCT                                                 >  SRR4302533.1333713/1‑94 (MQ=60)
                                              GACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTG                                                >  SRR4302533.3791617/1‑94 (MQ=60)
                                              GACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTG                                                >  SRR4302533.5144437/1‑94 (MQ=60)
                                              GACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTG                                                >  SRR4302533.4665045/1‑94 (MQ=60)
                                                CCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGAC                                              >  SRR4302533.4287083/1‑94 (MQ=60)
                                                CCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGAC                                              <  SRR4302533.1290734/94‑1 (MQ=60)
                                                    TTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAA                                          <  SRR4302533.238444/94‑1 (MQ=60)
                                                    TTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAA                                          <  SRR4302533.4682192/94‑1 (MQ=60)
                                                     TTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAAT                                         <  SRR4302533.916150/94‑1 (MQ=60)
                                                     TTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAAT                                         >  SRR4302533.5728939/1‑94 (MQ=60)
                                                       TGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCG                                       <  SRR4302533.3766401/94‑1 (MQ=60)
                                                       TGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCGGCAGGAAACCATCGCCTTCCTGACGCAATCG                                       <  SRR4302533.6242963/94‑1 (MQ=60)
                                                       TGCTGCTCAGCGCAAAAGCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCG                                       <  SRR4302533.5843536/94‑1 (MQ=60)
                                                         CTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGG                                     >  SRR4302533.2914390/1‑94 (MQ=60)
                                                          TGCTCAGCGCAAAATCAAAGATGCTGCCCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGC                                    <  SRR4302533.2206382/94‑1 (MQ=60)
                                                           GCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCT                                   <  SRR4302533.4822316/94‑1 (MQ=60)
                                                           GCTCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCT                                   <  SRR4302533.6162976/94‑1 (MQ=60)
                                                             TCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTAT                                 >  SRR4302533.5032405/1‑94 (MQ=60)
                                                             TCAGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTAT                                 >  SRR4302533.2502392/1‑94 (MQ=60)
                                                               AGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCA                               <  SRR4302533.4492759/94‑1 (MQ=60)
                                                               AGCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCA                               <  SRR4302533.4366212/94‑1 (MQ=60)
                                                                GCGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAG                              >  SRR4302533.4379810/1‑94 (MQ=60)
                                                                 CGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGT                             >  SRR4302533.4536597/1‑94 (MQ=60)
                                                                 CGCAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGT                             <  SRR4302533.1757876/94‑1 (MQ=60)
                                                                  GCAAAATCAAAGATTCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTT                            <  SRR4302533.2689308/94‑1 (MQ=60)
                                                                   CAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTT                           >  SRR4302533.4863665/1‑94 (MQ=60)
                                                                   CAAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTT                           >  SRR4302533.4110118/1‑94 (MQ=60)
                                                                    AAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTA                          >  SRR4302533.1773487/1‑94 (MQ=60)
                                                                    AAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTA                          >  SRR4302533.3650332/1‑94 (MQ=60)
                                                                    AAAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTA                          >  SRR4302533.4963819/1‑94 (MQ=60)
                                                                     AAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTAT                         <  SRR4302533.3187202/94‑1 (MQ=60)
                                                                     AAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTAT                         <  SRR4302533.2715908/94‑1 (MQ=60)
                                                                     AAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTAT                         <  SRR4302533.4791306/94‑1 (MQ=60)
                                                                     AAATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTAT                         >  SRR4302533.4648355/1‑94 (MQ=60)
                                                                      AATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATC                        <  SRR4302533.683735/94‑1 (MQ=60)
                                                                      AATCAAAGATGCGGACCTGCCGGGTCCGCAACAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATC                        <  SRR4302533.746367/94‑1 (MQ=60)
                                                                       ATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCG                       >  SRR4302533.5642315/1‑94 (MQ=60)
                                                                       ATCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCG                       <  SRR4302533.5554013/94‑1 (MQ=60)
                                                                        TCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCCATCGGGCTATCAGTTTATCGG                      >  SRR4302533.2193724/1‑94 (MQ=60)
                                                                        TCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGG                      <  SRR4302533.2515900/94‑1 (MQ=60)
                                                                        TCAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGG                      <  SRR4302533.4071901/94‑1 (MQ=60)
                                                                        ACAAAGATGCTTGCCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGG                      <  SRR4302533.4396785/94‑1 (MQ=60)
                                                                         CAAAGATGCTTACCTGCCGGGTCCGCAACAAAAACTCGATATCCTGCAGGCAACAATCGCCTTCCTGACGCAATCGGGCTATCAGCTTATCGGT                     >  SRR4302533.4488433/1‑94 (MQ=60)
                                                                         CAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGT                     >  SRR4302533.5543439/1‑94 (MQ=60)
                                                                         CAAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGT                     <  SRR4302533.1670214/94‑1 (MQ=60)
                                                                          AAAGATTCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTA                    <  SRR4302533.4579375/94‑1 (MQ=60)
                                                                          AAAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCATGAAACCATCGCCTTCCTGACGCAATCGCGCTATCAGTTTATCGGAA                    >  SRR4302533.6254695/1‑94 (MQ=60)
                                                                           AAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTAT                   <  SRR4302533.204409/94‑1 (MQ=60)
                                                                           AAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTAT                   <  SRR4302533.5912161/94‑1 (MQ=60)
                                                                           AAGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTAT                   <  SRR4302533.2485660/94‑1 (MQ=60)
                                                                            AGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATG                  >  SRR4302533.1385166/1‑94 (MQ=60)
                                                                            AGATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATG                  <  SRR4302533.3355773/94‑1 (MQ=60)
                                                                             GATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGG                 >  SRR4302533.4739431/1‑94 (MQ=60)
                                                                             GATACTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGG                 <  SRR4302533.4951583/94‑1 (MQ=60)
                                                                              ATGCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGA                >  SRR4302533.3163562/1‑94 (MQ=60)
                                                                                GCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATC              <  SRR4302533.4563838/94‑1 (MQ=60)
                                                                                GCTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATC              >  SRR4302533.421320/1‑94 (MQ=60)
                                                                                 CTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCA             <  SRR4302533.139164/94‑1 (MQ=60)
                                                                                 CTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCA             >  SRR4302533.764451/1‑94 (MQ=60)
                                                                                 CTGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCA             >  SRR4302533.6320964/1‑94 (MQ=60)
                                                                                  TGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCAC            >  SRR4302533.2190065/1‑94 (MQ=60)
                                                                                  TGACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCAC            >  SRR4302533.5027137/1‑94 (MQ=60)
                                                                                   GACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACT           <  SRR4302533.1868568/94‑1 (MQ=60)
                                                                                    ACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCTCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTT          <  SRR4302533.4574258/94‑1 (MQ=60)
                                                                                    ACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTT          >  SRR4302533.4860915/1‑94 (MQ=60)
                                                                                    ACCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTT          <  SRR4302533.5530991/94‑1 (MQ=60)
                                                                                     CCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTT         <  SRR4302533.1911278/94‑1 (MQ=60)
                                                                                     CCTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTT         >  SRR4302533.842030/1‑94 (MQ=60)
                                                                                      CTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTG        <  SRR4302533.4885010/94‑1 (MQ=60)
                                                                                      CTGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTG        >  SRR4302533.3540286/1‑94 (MQ=60)
                                                                                       TGCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGC       >  SRR4302533.4782277/1‑94 (MQ=60)
                                                                                        GCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCC      <  SRR4302533.406826/94‑1 (MQ=60)
                                                                                        GCCGGGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCC      >  SRR4302533.2734611/1‑94 (MQ=60)
                                                                                            GGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTC  >  SRR4302533.2060201/1‑94 (MQ=60)
                                                                                            |                                                                                             
AGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTC  >  NC_000913/4050316‑4050501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑