Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,051,170 +C intergenic (+172/+279) hemN → / ← glnG O2‑independent coproporphyrinogen III oxidase/GlnG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,051,1701.C99.7% 1107.9 / ‑4.1 391intergenic (+172/+279)hemN/glnGO2‑independent coproporphyrinogen III oxidase/GlnG
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (224/166);  total (224/167)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.55e-01

Only 100 of 423 total aligned reads displayed.

TGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCCATCTGAGCATGATGCCTGATGCGGCG‑TGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACA  >  NC_000913/4051077‑4051256
                                                                                              |                                                                                      
 gggCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcgc                                                                                        >  1:9579141/1‑92 (MQ=255)
 gggCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcgc                                                                                        <  1:2916914/93‑2 (MQ=255)
 gggCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcgc                                                                                        <  1:3333490/93‑2 (MQ=255)
    caacaaAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATgcggcgctgg                                                                                     <  2:8156754/93‑5 (MQ=255)
       caaAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTcg                                                                                  <  2:7874885/93‑1 (MQ=25)
       caaAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTcg                                                                                  >  1:2409359/1‑93 (MQ=21)
       caaAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTcg                                                                                  >  1:9135303/1‑93 (MQ=21)
       caaAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTcg                                                                                  >  2:6641154/1‑93 (MQ=25)
        aaaaCCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTcgc                                                                                 >  1:169979/1‑93 (MQ=25)
           aCCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTc                                                                              >  1:2310653/1‑93 (MQ=21)
              tGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTa                                                                           <  2:2458779/93‑1 (MQ=21)
                ttGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATc                                                                         <  2:5663060/93‑1 (MQ=18)
                ttGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATc                                                                         >  1:4375647/1‑93 (MQ=21)
                 tGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCa                                                                        <  2:3686645/93‑1 (MQ=21)
                 tGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCa                                                                        <  1:7825578/93‑1 (MQ=21)
                    aaTTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGc                                                                     <  2:6346990/93‑1 (MQ=18)
                     attattGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGcc                                                                    >  2:7765259/1‑93 (MQ=18)
                       tattGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTa                                                                  <  1:7337412/93‑1 (MQ=18)
                       tattGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTa                                                                  >  1:8837022/1‑93 (MQ=18)
                        attGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTAc                                                                 >  1:6927576/1‑93 (MQ=17)
                        attGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTAc                                                                 <  2:7957530/93‑1 (MQ=17)
                          tGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGa                                                               >  2:8330689/1‑93 (MQ=17)
                          tGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGa                                                               <  2:9280476/93‑1 (MQ=17)
                           gCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGAc                                                              >  1:5446771/1‑93 (MQ=18)
                           gCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGAc                                                              >  1:1338476/1‑93 (MQ=17)
                           gCTTTTGTAGGCCGGATAAGGCGTTTACGACGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCCGGCCTACGAc                                                              >  1:2498755/1‑93 (MQ=17)
                            tttttGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACt                                                             <  2:1871221/92‑1 (MQ=17)
                            ctcttGTAGGCCGGATAATGCGGTTGCGCCGCATCATG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACt                                                             <  1:8656529/90‑1 (MQ=17)
                            cTTTGGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACt                                                             <  2:4415906/93‑1 (MQ=17)
                              tttGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACttt                                                           <  2:1510005/93‑1 (MQ=17)
                               ttGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCAGCGCTGGTCGCGTCTTATCAGGCCTACGACTTtc                                                          <  1:4055755/93‑1 (MQ=18)
                                tGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTtct                                                         >  1:5009243/1‑93 (MQ=18)
                                tGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTtct                                                         >  2:4817598/1‑93 (MQ=18)
                                 gTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTtctc                                                        <  2:7195020/93‑1 (MQ=18)
                                  tAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTcc                                                       >  2:4167258/1‑93 (MQ=18)
                                   aGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCg                                                      >  2:5153008/1‑93 (MQ=18)
                                    ggCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGc                                                     <  2:6443173/93‑1 (MQ=18)
                                     gCCGGATAAGGCGTTTCCGCCGCTTCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCa                                                    <  1:3672548/93‑1 (MQ=18)
                                      ccGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCAt                                                   >  1:6248276/1‑93 (MQ=18)
                                        ggATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATcg                                                 >  2:841287/1‑92 (MQ=18)
                                          aTAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCgg                                               <  1:4854989/93‑1 (MQ=18)
                                           tAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGc                                              >  1:5723673/1‑93 (MQ=21)
                                            aaGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCa                                             <  1:4196348/93‑1 (MQ=18)
                                             aGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCAt                                            >  1:7226172/1‑93 (MQ=21)
                                             aGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCAt                                            >  2:4644112/1‑93 (MQ=21)
                                              ggCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATa                                           >  1:9363396/1‑93 (MQ=21)
                                              ggCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATa                                           >  2:215987/1‑93 (MQ=21)
                                              ggCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATa                                           <  2:3469917/93‑1 (MQ=21)
                                               gCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATaa                                          <  2:6802475/93‑1 (MQ=21)
                                               gCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATaa                                          >  1:3035256/1‑93 (MQ=21)
                                                 gTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAaac                                        <  2:8585180/93‑1 (MQ=21)
                                                  tttACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAaaca                                       >  1:6607989/1‑93 (MQ=21)
                                                   ttACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAaacaa                                      <  1:9439152/93‑1 (MQ=21)
                                                   ttACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAaacaa                                      <  2:3200228/93‑1 (MQ=21)
                                                   ttACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCAGGCATAaacaa                                      <  1:9881039/93‑1 (MQ=21)
                                                     aCGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACg                                    >  2:1124835/1‑93 (MQ=25)
                                                      cgccgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGa                                   <  2:2282217/93‑1 (MQ=25)
                                                       gccgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAg                                  <  1:3200960/93‑1 (MQ=25)
                                                         cgcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCt                                >  2:8876350/1‑93 (MQ=25)
                                                          gcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCtt                               >  2:8712597/1‑93 (MQ=37)
                                                          gcATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCtt                               >  2:7008370/1‑93 (MQ=37)
                                                           cATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTc                              <  2:1801579/93‑1 (MQ=37)
                                                           cATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTc                              <  1:8421422/93‑1 (MQ=37)
                                                            gtCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCa                             <  1:5103849/92‑1 (MQ=37)
                                                            aTCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCa                             >  2:4998159/1‑93 (MQ=37)
                                                            aTCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCa                             >  2:9699558/1‑93 (MQ=37)
                                                            aTCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCa                             >  1:9801128/1‑93 (MQ=37)
                                                            aTCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCa                             <  1:7636320/93‑1 (MQ=37)
                                                             tCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAg                            >  2:9530379/1‑93 (MQ=37)
                                                             tCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAg                            >  1:1537579/1‑93 (MQ=37)
                                                                aggcATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCt                          >  2:5577955/4‑93 (MQ=255)
                                                                 ggcATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTa                         >  1:9725663/3‑93 (MQ=255)
                                                                   cATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTaaa                       <  1:3795320/93‑1 (MQ=255)
                                                                    aTCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAAc                      <  2:5390520/93‑1 (MQ=255)
                                                                     tCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACa                     >  2:4028045/1‑93 (MQ=255)
                                                                     tCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACa                     <  2:2589815/93‑1 (MQ=255)
                                                                     tCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACa                     >  1:2653716/1‑93 (MQ=255)
                                                                     tCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACa                     <  2:6061358/93‑1 (MQ=255)
                                                                     tCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACa                     >  2:3755451/1‑93 (MQ=255)
                                                                     tCTGAGAATAATGCATGATGCGGCGCAGGTCGCGTATTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACa                     <  1:8923884/93‑1 (MQ=37)
                                                                       tGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGc                   >  1:3563841/1‑93 (MQ=255)
                                                                        gAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGcc                  >  1:10027679/1‑93 (MQ=255)
                                                                        gAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGcc                  >  1:7890896/1‑93 (MQ=255)
                                                                          gCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCa                >  2:6925675/1‑93 (MQ=255)
                                                                          gCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCa                >  2:1725277/1‑93 (MQ=255)
                                                                            atgatgCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAAt              >  2:477628/1‑93 (MQ=255)
                                                                            atgatgCCCGATGGGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAAt              <  1:4613016/93‑1 (MQ=38)
                                                                             tgatgCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATc             >  1:2023250/1‑93 (MQ=255)
                                                                             tgatgCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATc             >  1:5364360/1‑93 (MQ=255)
                                                                                tgCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCAtt          <  1:6709226/93‑1 (MQ=255)
                                                                                 gCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTg         <  2:1195685/93‑1 (MQ=255)
                                                                                 gCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTg         >  2:3076594/1‑93 (MQ=255)
                                                                                  ccTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGc        <  1:6399121/93‑1 (MQ=255)
                                                                                   cTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCg       >  1:4509306/1‑93 (MQ=255)
                                                                                   cTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCg       <  2:3860095/93‑1 (MQ=255)
                                                                                    tGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCgg      >  1:2854824/1‑93 (MQ=255)
                                                                                     gATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGc     >  1:5919915/1‑93 (MQ=255)
                                                                                     gATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGc     >  2:7489325/1‑93 (MQ=255)
                                                                                      aTGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGca    >  1:6127851/1‑93 (MQ=255)
                                                                                        gcggcgcTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGcaca  <  1:8193608/93‑1 (MQ=255)
                                                                                              |                                                                                      
TGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCCATCTGAGCATGATGCCTGATGCGGCG‑TGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACA  >  NC_000913/4051077‑4051256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 239 total aligned reads displayed.
CTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCCATCTGAGCATGATGCCTGATGCGGCG‑TGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACAGCACG  >  NC_000913/4051076‑4051263
                                                                                              |                                                                                              
CTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCG                                                                                                <  SRR4302540.5089035/94‑1 (MQ=60)
CTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCG                                                                                                <  SRR4302540.7684634/94‑1 (MQ=60)
CTGGGAAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCATTTACGGCGCAGCAGG‑CATCTGAGCATGATGCCTGATGCGGCG                                                                                                >  SRR4302540.7151/1‑94 (MQ=60)
 TGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCG‑C                                                                                              >  SRR4302540.9692263/1‑94 (MQ=60)
  GGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGct                                                                                              <  SRR4302540.2948840/94‑3 (MQ=60)
  GGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGct                                                                                              <  SRR4302540.2948837/94‑3 (MQ=60)
   GGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGctg                                                                                             <  SRR4302540.3340528/94‑4 (MQ=60)
   GGCAACAAAACCTGTTGAAATTATTGCTTGTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGctg                                                                                             <  SRR4302540.3340527/94‑4 (MQ=60)
    GCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGG                                                                                            <  SRR4302540.6414299/94‑1 (MQ=60)
     CAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGctggc                                                                                           >  SRR4302540.3235721/1‑89 (MQ=60)
      AACAAAACCTGTTGAAATTATTGCTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTC                                                                                          <  SRR4302540.7156127/94‑1 (MQ=60)
      AACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGGCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGgtgggc                                                                                          >  SRR4302540.6325779/1‑88 (MQ=60)
      AACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTC                                                                                          >  SRR4302540.9148507/1‑94 (MQ=60)
      AACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTC                                                                                          <  SRR4302540.5837588/94‑1 (MQ=60)
      AACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTC                                                                                          >  SRR4302540.840818/1‑94 (MQ=60)
      AACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTC                                                                                          >  SRR4302540.6325778/1‑94 (MQ=60)
       ACAACACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG                                                                                         <  SRR4302540.2448018/94‑1 (MQ=60)
       ACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG                                                                                         <  SRR4302540.342127/94‑1 (MQ=60)
       ACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG                                                                                         <  SRR4302540.3281027/94‑1 (MQ=60)
       ACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG                                                                                         >  SRR4302540.9245083/1‑94 (MQ=60)
       ACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG                                                                                         <  SRR4302540.9097008/94‑1 (MQ=60)
       ACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCG                                                                                         >  SRR4302540.2435335/1‑94 (MQ=60)
        CAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGctgggcgc                                                                                        >  SRR4302540.1713798/1‑86 (MQ=60)
        CAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGC                                                                                        <  SRR4302540.666347/94‑1 (MQ=60)
        CAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGC                                                                                        >  SRR4302540.171682/1‑94 (MQ=60)
        CAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGC                                                                                        >  SRR4302540.2220315/1‑94 (MQ=60)
        CAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGACGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGC                                                                                        >  SRR4302540.171681/1‑94 (MQ=60)
         AAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCG                                                                                       <  SRR4302540.3776255/94‑1 (MQ=60)
          AAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGT                                                                                      >  SRR4302540.3576001/1‑94 (MQ=60)
          AAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGT                                                                                      >  SRR4302540.3696122/1‑94 (MQ=60)
          AAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGT                                                                                      >  SRR4302540.2892505/1‑94 (MQ=60)
           AACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTC                                                                                     >  SRR4302540.9073019/1‑94 (MQ=60)
           AACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTC                                                                                     >  SRR4302540.2335525/1‑94 (MQ=60)
           AACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTC                                                                                     >  SRR4302540.8069309/1‑94 (MQ=60)
            ACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCT                                                                                    <  SRR4302540.3037627/94‑1 (MQ=60)
             CCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGTCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTT                                                                                   >  SRR4302540.9543509/1‑94 (MQ=60)
             CCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTT                                                                                   >  SRR4302540.9334884/1‑94 (MQ=60)
              CTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTA                                                                                  >  SRR4302540.6807138/1‑94 (MQ=60)
               TGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTAT                                                                                 >  SRR4302540.6753450/1‑94 (MQ=60)
                GTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATC                                                                                >  SRR4302540.4423163/1‑94 (MQ=60)
                GTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATC                                                                                <  SRR4302540.9600563/94‑1 (MQ=60)
                GTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATC                                                                                >  SRR4302540.7510005/1‑94 (MQ=60)
                GTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATC                                                                                <  SRR4302540.1845786/94‑1 (MQ=60)
                GTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATC                                                                                >  SRR4302540.8197665/1‑94 (MQ=60)
                GTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATC                                                                                >  SRR4302540.7037079/1‑94 (MQ=60)
                 TTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCA                                                                               <  SRR4302540.9864747/94‑1 (MQ=60)
                  TGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAG                                                                              <  SRR4302540.7917027/94‑1 (MQ=60)
                  TGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAG                                                                              <  SRR4302540.9815259/94‑1 (MQ=60)
                  TGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAG                                                                              >  SRR4302540.5702170/1‑94 (MQ=60)
                  TGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCAGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAG                                                                              <  SRR4302540.2087486/94‑1 (MQ=60)
                    AAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGC                                                                            >  SRR4302540.5393984/1‑94 (MQ=60)
                       TTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTA                                                                         >  SRR4302540.8939231/1‑94 (MQ=60)
                        TATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTAC                                                                        >  SRR4302540.861252/1‑94 (MQ=60)
                        TATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTAC                                                                        >  SRR4302540.7004670/1‑94 (MQ=60)
                        TATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTAC                                                                        >  SRR4302540.637722/1‑94 (MQ=60)
                        TATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTAC                                                                        <  SRR4302540.6750906/94‑1 (MQ=60)
                        TATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTAC                                                                        <  SRR4302540.3107189/94‑1 (MQ=60)
                        TATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTAC                                                                        <  SRR4302540.7419298/94‑1 (MQ=60)
                         ATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACG                                                                       <  SRR4302540.3763415/94‑1 (MQ=60)
                         ATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACG                                                                       <  SRR4302540.10158548/94‑1 (MQ=60)
                         ATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACG                                                                       <  SRR4302540.2605087/94‑1 (MQ=60)
                         ATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACG                                                                       <  SRR4302540.7741383/94‑1 (MQ=60)
                           TGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGAC                                                                     >  SRR4302540.6998281/1‑94 (MQ=60)
                           TGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGAC                                                                     >  SRR4302540.5506709/1‑94 (MQ=60)
                           TGCTTTTGTAGGCCGGATAAGGCGTTTACGACGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCCGGCCTACGAC                                                                     >  SRR4302540.2525701/1‑94 (MQ=60)
                            GCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACT                                                                    <  SRR4302540.2683538/94‑1 (MQ=60)
                            GCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACT                                                                    >  SRR4302540.5376747/1‑94 (MQ=60)
                             TTTTTGGAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTT                                                                   <  SRR4302540.7982945/94‑1 (MQ=60)
                               TTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTC                                                                 >  SRR4302540.9585797/1‑94 (MQ=60)
                                TTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCT                                                                <  SRR4302540.7967574/94‑1 (MQ=60)
                                TTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCT                                                                <  SRR4302540.871947/94‑1 (MQ=60)
                                TTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCAGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCT                                                                <  SRR4302540.4099482/94‑1 (MQ=60)
                                TTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGACGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCT                                                                <  SRR4302540.9450869/94‑1 (MQ=60)
                                  GTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCC                                                              <  SRR4302540.3371590/94‑1 (MQ=60)
                                  GTAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCC                                                              <  SRR4302540.5539847/94‑1 (MQ=60)
                                   TAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCG                                                             <  SRR4302540.5111334/94‑1 (MQ=60)
                                   TAGGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCG                                                             >  SRR4302540.6638014/1‑94 (MQ=60)
                                   TAGGCCGGATAAGGCGATTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCG                                                             <  SRR4302540.7799173/94‑1 (MQ=60)
                                     GGCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCA                                                           >  SRR4302540.10111948/1‑94 (MQ=60)
                                      GCCGGATAAGGCGTTTCCGCCGCTTCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCAT                                                          <  SRR4302540.3712712/94‑1 (MQ=60)
                                      GCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCAT                                                          <  SRR4302540.2794860/94‑1 (MQ=60)
                                      GCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCAT                                                          <  SRR4302540.9943543/94‑1 (MQ=60)
                                      GCCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCAT                                                          >  SRR4302540.6317809/1‑94 (MQ=60)
                                       CTGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATC                                                         <  SRR4302540.9593751/94‑1 (MQ=60)
                                       CCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATC                                                         >  SRR4302540.433117/1‑94 (MQ=60)
                                       CCGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATC                                                         >  SRR4302540.5497445/1‑94 (MQ=60)
                                        CGGATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCC                                                        >  SRR4302540.6620057/1‑94 (MQ=60)
                                        CGGATAAGGCGTTTACGCCGCATCA‑GCCATCTGAGCATGATGCATGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCC                                                        <  SRR4302540.9920729/94‑1 (MQ=60)
                                        CGGATAAGGCGTTTACGACGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCC                                                        >  SRR4302540.4563875/1‑94 (MQ=60)
                                         GGATAAGGCGTTTACGCCACATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCG                                                       >  SRR4302540.452543/1‑94 (MQ=60)
                                          GATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGG                                                      <  SRR4302540.7338669/94‑1 (MQ=60)
                                           ATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGTCTTTCTCCGCATCCGGC                                                     >  SRR4302540.7530637/1‑94 (MQ=60)
                                           ATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGC                                                     >  SRR4302540.8898330/1‑94 (MQ=60)
                                           ATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGC                                                     >  SRR4302540.4441151/1‑94 (MQ=60)
                                           ATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGC                                                     >  SRR4302540.5786678/1‑94 (MQ=60)
                                           ATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGC                                                     <  SRR4302540.4907032/94‑1 (MQ=60)
                                           ATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGC                                                     >  SRR4302540.8467273/1‑94 (MQ=60)
                                           ATAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGC                                                     >  SRR4302540.5879680/1‑94 (MQ=60)
                                            TAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCA                                                    <  SRR4302540.9544252/94‑1 (MQ=60)
                                            TAAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCA                                                    >  SRR4302540.5782730/1‑94 (MQ=60)
                                             AAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCAT                                                   <  SRR4302540.4241994/94‑1 (MQ=60)
                                             AAGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCAT                                                   >  SRR4302540.7306745/1‑94 (MQ=60)
                                             AAGGCGTTTACGACGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTGCGCATCCGGAAT                                                   >  SRR4302540.7306746/1‑94 (MQ=60)
                                              AGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATA                                                  >  SRR4302540.9474896/1‑94 (MQ=60)
                                              AGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATA                                                  >  SRR4302540.9474897/1‑94 (MQ=60)
                                              AGGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATA                                                  >  SRR4302540.630810/1‑94 (MQ=60)
                                               GGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAA                                                 >  SRR4302540.3068567/1‑94 (MQ=60)
                                               GGCGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAA                                                 >  SRR4302540.6190582/1‑94 (MQ=60)
                                                 CGTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAAC                                               >  SRR4302540.4775371/1‑94 (MQ=60)
                                                  GTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACA                                              >  SRR4302540.2522184/1‑94 (MQ=60)
                                                  GTTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACA                                              >  SRR4302540.2431278/1‑94 (MQ=60)
                                                   TTTACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAA                                             <  SRR4302540.4200251/94‑1 (MQ=60)
                                                   TGTACGCCGCATCAGG‑CAACTGAGCATGAGTCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAA                                             <  SRR4302540.2348158/94‑1 (MQ=60)
                                                     TACGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACG                                           <  SRR4302540.9886736/94‑1 (MQ=60)
                                                       CGCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAG                                         <  SRR4302540.7106743/94‑1 (MQ=60)
                                                        GCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGC                                        >  SRR4302540.1904322/1‑94 (MQ=60)
                                                        GCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGC                                        <  SRR4302540.1586310/94‑1 (MQ=60)
                                                        GCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGC                                        >  SRR4302540.4636848/1‑94 (MQ=60)
                                                        GCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGC                                        <  SRR4302540.3235722/94‑1 (MQ=60)
                                                        GCCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGC                                        <  SRR4302540.5073362/94‑1 (MQ=60)
                                                         CCGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGCCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCT                                       <  SRR4302540.7899859/94‑1 (MQ=60)
                                                          CGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTT                                      >  SRR4302540.3961662/1‑94 (MQ=60)
                                                          CGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTT                                      <  SRR4302540.4219846/94‑1 (MQ=60)
                                                          CGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTT                                      >  SRR4302540.3115819/1‑94 (MQ=60)
                                                          CGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTT                                      >  SRR4302540.6730829/1‑94 (MQ=60)
                                                          CGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTT                                      >  SRR4302540.3561872/1‑94 (MQ=60)
                                                          CGCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTT                                      <  SRR4302540.6313194/94‑1 (MQ=60)
                                                           GCATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTC                                     >  SRR4302540.1455769/1‑94 (MQ=60)
                                                            CATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCA                                    <  SRR4302540.9628277/94‑1 (MQ=60)
                                                            CATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCA                                    >  SRR4302540.9915883/1‑94 (MQ=60)
                                                            CATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCA                                    <  SRR4302540.4362400/94‑1 (MQ=60)
                                                            CATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCA                                    <  SRR4302540.8519175/94‑1 (MQ=60)
                                                             ATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAG                                   >  SRR4302540.8164195/1‑94 (MQ=60)
                                                             ATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAG                                   <  SRR4302540.7722232/94‑1 (MQ=60)
                                                             ATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAG                                   >  SRR4302540.1554583/1‑94 (MQ=60)
                                                             ATCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAG                                   >  SRR4302540.4189652/1‑94 (MQ=60)
                                                              gtcaggCATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAG                                   <  SRR4302540.5158823/88‑1 (MQ=60)
                                                              gtcaggCATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAG                                   <  SRR4302540.5159099/88‑1 (MQ=60)
                                                              TCAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGC                                  <  SRR4302540.7311090/94‑1 (MQ=60)
                                                               CAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCT                                 <  SRR4302540.4583875/94‑1 (MQ=60)
                                                               CAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCT                                 >  SRR4302540.5989592/1‑94 (MQ=60)
                                                               CAGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCT                                 <  SRR4302540.9255223/94‑1 (MQ=60)
                                                                gaggcatttgagaataatgctcGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCT                                 <  SRR4302540.8988924/72‑1 (MQ=60)
                                                                AGG‑CATCTGAGCATGATGCGTGATGCGGGGCTGGTCGCGTGTTATCAGGGCTCCGACTTTGTCCGCATCCGGCATAAACAACGAGCTTCAGCTA                                >  SRR4302540.9839892/1‑94 (MQ=53)
                                                                AGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTA                                >  SRR4302540.9839893/1‑94 (MQ=60)
                                                                AGG‑CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTA                                >  SRR4302540.3965051/1‑94 (MQ=60)
                                                                    CATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAAC                             <  SRR4302540.3836683/94‑1 (MQ=60)
                                                                     ATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACA                            >  SRR4302540.7959672/1‑94 (MQ=60)
                                                                     ATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACA                            >  SRR4302540.900737/1‑94 (MQ=60)
                                                                     ATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACA                            >  SRR4302540.9968360/1‑94 (MQ=60)
                                                                     ATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACA                            <  SRR4302540.1384877/94‑1 (MQ=60)
                                                                     ATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACA                            >  SRR4302540.3679605/1‑94 (MQ=60)
                                                                     ATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACA                            >  SRR4302540.1415155/1‑94 (MQ=60)
                                                                     ATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACA                            >  SRR4302540.5488999/1‑94 (MQ=60)
                                                                     ATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACA                            >  SRR4302540.2121581/1‑94 (MQ=60)
                                                                     ATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACA                            >  SRR4302540.6115376/1‑94 (MQ=60)
                                                                     ATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACA                            >  SRR4302540.2682505/1‑94 (MQ=60)
                                                                     ATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCAGGCATAAACAACGAGCCTCAGCTAAACA                            >  SRR4302540.4665161/1‑94 (MQ=60)
                                                                     ATCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCAGAAGCAGGCATAAACAACGAGATTCAGCTACACA                            >  SRR4302540.900738/1‑94 (MQ=57)
                                                                      TCTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAG                           >  SRR4302540.2433939/1‑94 (MQ=60)
                                                                      TCTGAGAATAATGCATGATGCGGCGCAGGTCGCGTATTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAG                           <  SRR4302540.9027524/94‑1 (MQ=60)
                                                                       CTGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGC                          >  SRR4302540.3602769/1‑94 (MQ=60)
                                                                        TGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCC                         >  SRR4302540.7982992/1‑94 (MQ=60)
                                                                        TGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCC                         >  SRR4302540.10144349/1‑94 (MQ=60)
                                                                          AGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCA                       <  SRR4302540.3999643/94‑1 (MQ=60)
                                                                          AGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCA                       <  SRR4302540.8863495/94‑1 (MQ=60)
                                                                             ATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATC                    >  SRR4302540.2045114/1‑94 (MQ=60)
                                                                             ATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATC                    <  SRR4302540.4662517/94‑1 (MQ=60)
                                                                             ATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATC                    >  SRR4302540.5859122/1‑94 (MQ=60)
                                                                             ATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATC                    >  SRR4302540.5423289/1‑94 (MQ=60)
                                                                             ATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATA                    >  SRR4302540.3047994/1‑94 (MQ=60)
                                                                             ATGATGCCCGATGGGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATC                    <  SRR4302540.4662516/94‑1 (MQ=60)
                                                                              TGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCACAAACAACGAGCTTCAGCTAAACAGCCCAATCA                   >  SRR4302540.8984258/1‑94 (MQ=60)
                                                                               GATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCAT                  <  SRR4302540.5286439/94‑1 (MQ=60)
                                                                                ATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATT                 <  SRR4302540.2894037/94‑1 (MQ=60)
                                                                                ATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATT                 <  SRR4302540.8682336/94‑1 (MQ=60)
                                                                                 TGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTG                <  SRR4302540.4993947/94‑1 (MQ=60)
                                                                                 TGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTG                <  SRR4302540.6783263/94‑1 (MQ=60)
                                                                                 TGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCACAATCATTG                >  SRR4302540.7584173/1‑94 (MQ=60)
                                                                                  GCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGC               <  SRR4302540.1303513/94‑1 (MQ=60)
                                                                                   CCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTTCGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCG              <  SRR4302540.2725255/94‑1 (MQ=60)
                                                                                   CCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAACCAACGAGCTTCAGCTAAACAGCCCAATCATTGCG              >  SRR4302540.2753722/1‑94 (MQ=60)
                                                                                   CCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCG              <  SRR4302540.6470357/94‑1 (MQ=60)
                                                                                    CTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGG             >  SRR4302540.4426855/1‑94 (MQ=60)
                                                                                    CTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGG             >  SRR4302540.8885871/1‑94 (MQ=60)
                                                                                    CTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGG             >  SRR4302540.5020722/1‑94 (MQ=60)
                                                                                    CTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCCCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGG             >  SRR4302540.1144458/1‑94 (MQ=60)
                                                                                     TGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGC            >  SRR4302540.2551281/1‑94 (MQ=60)
                                                                                     TGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGC            >  SRR4302540.5985315/1‑94 (MQ=60)
                                                                                     TGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGC            >  SRR4302540.2924666/1‑94 (MQ=60)
                                                                                     TGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGC            >  SRR4302540.499601/1‑94 (MQ=60)
                                                                                      GATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCA           >  SRR4302540.6196106/1‑94 (MQ=60)
                                                                                       ATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCAC          >  SRR4302540.9700087/1‑94 (MQ=60)
                                                                                         GCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACAC        <  SRR4302540.8288814/94‑1 (MQ=60)
                                                                                         GCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACAC        >  SRR4302540.5276177/1‑94 (MQ=60)
                                                                                          CGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACA       >  SRR4302540.1024783/1‑94 (MQ=60)
                                                                                             CC‑TGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACAGCAC   <  SRR4302540.7227829/94‑1 (MQ=60)
                                                                                              C‑TGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACAGCACG  >  SRR4302540.8150209/1‑94 (MQ=60)
                                                                                              C‑TGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACAGCACG  <  SRR4302540.1756902/94‑1 (MQ=60)
                                                                                              C‑TGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACAGCACG  <  SRR4302540.295366/94‑1 (MQ=60)
                                                                                              |                                                                                              
CTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCCATCTGAGCATGATGCCTGATGCGGCG‑TGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACAGCACG  >  NC_000913/4051076‑4051263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑