Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,083,471 A→G intergenic (‑69/‑125) fdoG ← / → fdhD formate dehydrogenase‑O, major subunit/affects formate dehydrogenase‑N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,083,4710AG99.8% 1572.6 / ‑6.0 485intergenic (‑69/‑125)fdoG/fdhDformate dehydrogenase‑O, major subunit/affects formate dehydrogenase‑N
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (292/192);  total (292/193)
Fisher's exact test for biased strand distribution p-value = 3.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 558 total aligned reads displayed.

ACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAA  >  NC_000913/4083381‑4083560
                                                                                          |                                                                                         
aCTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAgggagtgggggt                                                                                         <  2:7115864/93‑1 (MQ=255)
  tGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTa                                                                                       >  1:6098189/1‑93 (MQ=255)
  tGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTa                                                                                       <  1:5056918/93‑1 (MQ=255)
     cTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTAttt                                                                                    >  2:1836028/1‑93 (MQ=255)
      ttCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTAtttt                                                                                   >  2:3653596/1‑93 (MQ=255)
      ttCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTAtttt                                                                                   >  2:8884031/1‑93 (MQ=255)
         tgctgACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCt                                                                                <  1:9805313/93‑1 (MQ=255)
         tgctgACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCt                                                                                >  1:5507858/1‑93 (MQ=255)
          gctgACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCtt                                                                               >  2:6658531/1‑93 (MQ=255)
           ctgACCTGCATGTATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCttt                                                                              <  2:9401887/93‑1 (MQ=255)
            tgACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTg                                                                             <  2:3006653/93‑1 (MQ=255)
              aCCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCg                                                                           >  1:3293701/1‑93 (MQ=255)
               ccTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCgg                                                                          <  2:5400278/93‑1 (MQ=255)
               ccTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCgg                                                                          >  1:3904507/1‑93 (MQ=255)
                 tGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGaa                                                                        >  1:2119656/1‑93 (MQ=255)
                 cgCATGGATTGCTCATTGTTTCGACATTGTCACGTCCCATTTACATTCGCGTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGaa                                                                        <  1:6465829/92‑1 (MQ=255)
                  gCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAg                                                                       <  2:8614939/93‑1 (MQ=255)
                   cATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAgg                                                                      <  1:2381451/93‑1 (MQ=255)
                   cATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAgg                                                                      <  2:6248940/93‑1 (MQ=255)
                       gATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGTAAGGGGcc                                                                  >  1:4698925/1‑93 (MQ=255)
                       gATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGcc                                                                  >  1:245482/1‑93 (MQ=255)
                       gATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGcc                                                                  <  1:8262041/93‑1 (MQ=255)
                       gATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGcc                                                                  >  1:1294796/1‑93 (MQ=255)
                        aTTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCg                                                                 <  1:5257749/93‑1 (MQ=255)
                          tGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCa                                                               >  1:788307/1‑93 (MQ=255)
                          tGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCa                                                               >  1:6918938/1‑93 (MQ=255)
                          tGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCa                                                               >  2:3365738/1‑93 (MQ=255)
                            cTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCaaa                                                             >  2:1100212/1‑93 (MQ=255)
                            cTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCaaa                                                             <  2:9565900/93‑1 (MQ=255)
                              ccTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAgg                                                           <  2:2904005/93‑1 (MQ=255)
                              ccTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAgg                                                           <  2:815512/93‑1 (MQ=255)
                                ttGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTc                                                         <  2:2769989/93‑1 (MQ=255)
                                ttGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTc                                                         >  2:4580822/1‑93 (MQ=255)
                                ttGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTc                                                         >  1:4893782/1‑93 (MQ=255)
                                ttGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTc                                                         >  2:4833301/1‑93 (MQ=255)
                                ttGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGCTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTc                                                         >  1:4395290/1‑93 (MQ=255)
                                 tGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTcc                                                        <  2:2571203/93‑1 (MQ=255)
                                   tttCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAg                                                      >  2:235666/1‑93 (MQ=255)
                                       gACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAAtt                                                  >  1:3143656/1‑93 (MQ=255)
                                        aCATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTg                                                 >  2:9844253/1‑93 (MQ=255)
                                          aTTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGc                                               >  1:8638434/1‑93 (MQ=255)
                                           ttGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGTAAGGGGCCGCAAAGGTCCAGAATTGGCt                                              >  1:8953685/1‑93 (MQ=255)
                                           ttGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCt                                              >  2:3228351/1‑93 (MQ=255)
                                           ttGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCt                                              >  1:4024081/1‑93 (MQ=255)
                                           ttGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCt                                              >  2:7021639/1‑93 (MQ=255)
                                            tGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCta                                             >  1:3009269/1‑92 (MQ=255)
                                            tGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTc                                             <  1:7232577/93‑1 (MQ=255)
                                             gTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCa                                            <  2:5673268/93‑1 (MQ=255)
                                              tCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCaa                                           >  1:9286737/1‑93 (MQ=255)
                                              tCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCaa                                           >  1:1237885/1‑93 (MQ=255)
                                              tCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCaa                                           <  1:1999651/93‑1 (MQ=255)
                                               cACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAAt                                          <  1:938085/93‑1 (MQ=255)
                                               cACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAAt                                          <  2:7281899/93‑1 (MQ=255)
                                               cACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAAt                                          >  1:2742564/1‑93 (MQ=255)
                                                 cGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAAttt                                        >  1:1695555/1‑93 (MQ=255)
                                                  gTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTc                                       >  1:9913028/1‑93 (MQ=255)
                                                   tCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTcc                                      >  1:1097430/1‑93 (MQ=255)
                                                   tCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTcc                                      >  1:9632622/1‑93 (MQ=255)
                                                   tCCCATTTACATACGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTcc                                      >  2:1988821/1‑93 (MQ=255)
                                                    cccATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTccc                                     >  2:9217303/1‑93 (MQ=255)
                                                     ccATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCt                                    >  2:6869955/1‑93 (MQ=255)
                                                      cATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtc                                   >  2:5032195/1‑93 (MQ=255)
                                                       aTTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTAGTTTTCGTTGTGGGAGGGGCCGCAACGGTCCAGGATTGGCTCAATCTCCCtca                                  >  1:5477533/1‑92 (MQ=255)
                                                        tttACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtcca                                 >  1:5887220/1‑93 (MQ=255)
                                                        tttACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtcca                                 >  1:4202430/1‑93 (MQ=255)
                                                        tttACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtcca                                 >  1:4605834/1‑93 (MQ=255)
                                                           aCATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtccatcc                              <  1:6676922/93‑1 (MQ=255)
                                                           aCATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtccatcc                              <  2:3470957/93‑1 (MQ=255)
                                                            cATTCGCTTGCTGCGTGTGCGGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtgcatcca                             >  2:3855307/1‑93 (MQ=255)
                                                            cATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtccatcca                             <  1:3480114/93‑1 (MQ=255)
                                                            cATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtccatcca                             >  2:6473697/1‑93 (MQ=255)
                                                             aTTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAg                            >  1:3571127/1‑93 (MQ=255)
                                                              ttCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTTCCTCCATCCAgg                           >  1:7978338/1‑93 (MQ=255)
                                                              ttCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAgg                           >  1:1022097/1‑93 (MQ=255)
                                                               tCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAATGTCCAGAATTGGCTCAATTACCCTCCATCCAGGa                          <  2:7407751/93‑1 (MQ=255)
                                                                 gCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAg                        >  2:413204/1‑93 (MQ=255)
                                                                 gCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAg                        <  1:9726213/93‑1 (MQ=255)
                                                                  cTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAgg                       >  1:7635789/1‑93 (MQ=255)
                                                                   ttGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAggg                      <  2:4708649/93‑1 (MQ=255)
                                                                   ttGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAggg                      <  1:1478515/93‑1 (MQ=255)
                                                                   ttGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAggg                      <  1:6271254/93‑1 (MQ=255)
                                                                      ctgcGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGttt                   <  2:839402/93‑1 (MQ=255)
                                                                        gcGTGTTCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGt                 <  2:7683555/93‑1 (MQ=255)
                                                                        gcGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGt                 >  2:4306070/1‑93 (MQ=255)
                                                                         cGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTa                >  1:7481808/1‑93 (MQ=255)
                                                                         cGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTa                <  1:188289/93‑1 (MQ=255)
                                                                           tgtgCAGGGAGTGGGGGTTATTTTTTTTTGCGGAAGGGGCCGCAAAGGTCCGGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTGGTaaa              >  2:141467/1‑92 (MQ=255)
                                                                           tgtgCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAAc              >  1:7266792/1‑93 (MQ=255)
                                                                           tgtgCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAAc              >  2:7818011/1‑93 (MQ=255)
                                                                            gtgCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACa             <  1:7453097/93‑1 (MQ=255)
                                                                             tgCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAg            >  1:8418194/1‑93 (MQ=255)
                                                                             tgCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAg            >  1:6342655/1‑93 (MQ=255)
                                                                                aGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAAt         <  1:5719092/93‑1 (MQ=255)
                                                                                   gagtgggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATAcc      >  1:2557437/1‑93 (MQ=255)
                                                                                    agtgggggtTATTTTTCTTTGCGGAAGGAGCCGCAAAGGTCCAGAATTGGCTCAATTTCCATCCATCCAGGAAGGGTTTGTAACAGAATAcaa     >  1:8491665/1‑91 (MQ=255)
                                                                                     gtgggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCAt    >  2:2144222/1‑93 (MQ=255)
                                                                                      tgggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATa   >  2:1690905/1‑93 (MQ=255)
                                                                                      tgggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATa   >  2:5907000/1‑93 (MQ=255)
                                                                                      tgggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATa   >  2:5267506/1‑93 (MQ=255)
                                                                                       gggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATaa  >  1:3733416/1‑93 (MQ=255)
                                                                                          |                                                                                         
ACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAA  >  NC_000913/4083381‑4083560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 333 total aligned reads displayed.
AGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTT  >  NC_000913/4083378‑4083564
                                                                                             |                                                                                             
AGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGG                                                                                               <  SRR4302540.2140214/94‑1 (MQ=60)
 GAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGG                                                                                              <  SRR4302540.9355017/94‑1 (MQ=60)
   ACTTCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTT                                                                                            <  SRR4302540.3406263/94‑1 (MQ=60)
   ACTCCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTT                                                                                            <  SRR4302540.5478648/94‑1 (MQ=60)
    CTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTA                                                                                           >  SRR4302540.1053196/1‑94 (MQ=60)
    CTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTA                                                                                           >  SRR4302540.6166079/1‑94 (MQ=60)
     TGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTAT                                                                                          >  SRR4302540.834809/1‑94 (MQ=60)
       CCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTT                                                                                        >  SRR4302540.7416775/1‑94 (MQ=60)
        CTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTT                                                                                       >  SRR4302540.5431205/1‑94 (MQ=60)
        CTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTT                                                                                       >  SRR4302540.9994803/1‑94 (MQ=60)
        CTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTT                                                                                       >  SRR4302540.3507061/1‑94 (MQ=60)
           CTGCTGGCCTGCATGGATTGCTCGTTGTTTCGACATTGTCACGTCCCTTTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCT                                                                                    <  SRR4302540.754937/94‑1 (MQ=60)
           CTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCT                                                                                    >  SRR4302540.4672070/1‑94 (MQ=60)
           CTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCT                                                                                    >  SRR4302540.5568518/1‑94 (MQ=60)
           CTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCT                                                                                    >  SRR4302540.8330618/1‑94 (MQ=60)
           CTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCT                                                                                    >  SRR4302540.2000986/1‑94 (MQ=60)
           CTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGCTATTTTTCT                                                                                    >  SRR4302540.9870550/1‑94 (MQ=60)
            TGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTT                                                                                   <  SRR4302540.9920091/94‑1 (MQ=60)
            TGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTT                                                                                   >  SRR4302540.1179458/1‑94 (MQ=60)
            TGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTT                                                                                   <  SRR4302540.5434461/94‑1 (MQ=60)
             GCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTT                                                                                  <  SRR4302540.5597545/94‑1 (MQ=60)
              CTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTG                                                                                 <  SRR4302540.6440228/94‑1 (MQ=60)
               TGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGC                                                                                >  SRR4302540.34016/1‑94 (MQ=60)
               TGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGC                                                                                >  SRR4302540.2544930/1‑94 (MQ=60)
               TGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGC                                                                                >  SRR4302540.6211047/1‑94 (MQ=60)
               TGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGC                                                                                >  SRR4302540.9704463/1‑94 (MQ=60)
               TGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGC                                                                                >  SRR4302540.5101596/1‑94 (MQ=60)
               TGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGC                                                                                <  SRR4302540.3588084/94‑1 (MQ=60)
                GACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCG                                                                               >  SRR4302540.3329742/1‑94 (MQ=60)
                GACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCG                                                                               <  SRR4302540.8760992/94‑1 (MQ=60)
                GACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCG                                                                               <  SRR4302540.4308567/94‑1 (MQ=60)
                GACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCG                                                                               <  SRR4302540.1291813/94‑1 (MQ=60)
                GACCTGCATGGAGTGCTCCTTGTTTCGACAGTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCG                                                                               <  SRR4302540.1291812/94‑1 (MQ=60)
                 ACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGG                                                                              >  SRR4302540.3946850/1‑94 (MQ=60)
                  CCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGA                                                                             >  SRR4302540.700459/1‑94 (MQ=60)
                   CTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAG                                                                            >  SRR4302540.9411241/1‑94 (MQ=60)
                   CTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAA                                                                            >  SRR4302540.3671651/1‑94 (MQ=60)
                   CTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAA                                                                            >  SRR4302540.5011613/1‑94 (MQ=60)
                   CTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAA                                                                            <  SRR4302540.9465968/94‑1 (MQ=60)
                   CTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAA                                                                            >  SRR4302540.2254625/1‑94 (MQ=60)
                   CTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAA                                                                            <  SRR4302540.6344873/94‑1 (MQ=60)
                   CTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAA                                                                            >  SRR4302540.6598306/1‑94 (MQ=60)
                    ctCATGGACTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAG                                                                           <  SRR4302540.133564/92‑1 (MQ=60)
                    TGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAG                                                                           <  SRR4302540.2594446/94‑1 (MQ=60)
                    TGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAG                                                                           >  SRR4302540.431541/1‑94 (MQ=60)
                    TGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAG                                                                           >  SRR4302540.5124136/1‑94 (MQ=60)
                    TGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAG                                                                           <  SRR4302540.133563/94‑1 (MQ=60)
                    CGCATGGATTGCTCATTGTTTCGACATTGTCACGTCCCATTTACATTCGCGTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAG                                                                           <  SRR4302540.6537783/94‑1 (MQ=60)
                     GCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGG                                                                          <  SRR4302540.5585798/94‑1 (MQ=60)
                     GCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGG                                                                          <  SRR4302540.8651492/94‑1 (MQ=60)
                     GCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGG                                                                          <  SRR4302540.6068604/94‑1 (MQ=60)
                     GCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGG                                                                          <  SRR4302540.6068605/94‑1 (MQ=60)
                      CATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGG                                                                         <  SRR4302540.2407109/94‑1 (MQ=60)
                      CATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGG                                                                         >  SRR4302540.5204368/1‑94 (MQ=60)
                      CATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGG                                                                         >  SRR4302540.6456404/1‑94 (MQ=60)
                       ATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGG                                                                        >  SRR4302540.4845266/1‑94 (MQ=60)
                       ATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGG                                                                        <  SRR4302540.2319433/94‑1 (MQ=60)
                       ATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGG                                                                        >  SRR4302540.9936454/1‑94 (MQ=60)
                        TGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGC                                                                       >  SRR4302540.3226826/1‑94 (MQ=60)
                         GGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCC                                                                      >  SRR4302540.247859/1‑94 (MQ=60)
                         GGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCC                                                                      >  SRR4302540.1308197/1‑94 (MQ=60)
                         GGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCC                                                                      <  SRR4302540.7559362/94‑1 (MQ=60)
                         GGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCC                                                                      <  SRR4302540.4540529/94‑1 (MQ=60)
                          GATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCG                                                                     >  SRR4302540.4673055/1‑94 (MQ=60)
                           ATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGC                                                                    >  SRR4302540.7834141/1‑94 (MQ=60)
                            TTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCA                                                                   >  SRR4302540.796689/1‑94 (MQ=60)
                            TTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCA                                                                   >  SRR4302540.6995925/1‑94 (MQ=60)
                            TTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCA                                                                   >  SRR4302540.2474445/1‑94 (MQ=60)
                             TGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAA                                                                  >  SRR4302540.233665/1‑94 (MQ=60)
                              GCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAA                                                                 <  SRR4302540.10055133/94‑1 (MQ=60)
                              GCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAA                                                                 >  SRR4302540.1686059/1‑94 (MQ=60)
                               CTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAG                                                                <  SRR4302540.8855284/94‑1 (MQ=60)
                               CTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAG                                                                <  SRR4302540.3277540/94‑1 (MQ=60)
                                TCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGG                                                               >  SRR4302540.4761600/1‑94 (MQ=60)
                                TCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGG                                                               <  SRR4302540.20097/94‑1 (MQ=60)
                                  CTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTC                                                             >  SRR4302540.4946411/1‑94 (MQ=60)
                                  CTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGCTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTC                                                             >  SRR4302540.4442977/1‑94 (MQ=60)
                                   TTGTTTCGACATTGTCACGTCCCATTTACATGCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCC                                                            <  SRR4302540.7457333/94‑1 (MQ=60)
                                    TGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCA                                                           >  SRR4302540.4474717/1‑94 (MQ=60)
                                    TGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCA                                                           >  SRR4302540.9120882/1‑94 (MQ=60)
                                    TGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCA                                                           >  SRR4302540.1115621/1‑94 (MQ=60)
                                     GTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAG                                                          >  SRR4302540.1036998/1‑94 (MQ=60)
                                      TTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGA                                                         >  SRR4302540.6906975/1‑94 (MQ=60)
                                       TTCGACAGTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAA                                                        <  SRR4302540.8064594/94‑1 (MQ=60)
                                        TCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAAT                                                       <  SRR4302540.1819259/94‑1 (MQ=60)
                                         CGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATT                                                      <  SRR4302540.7547391/94‑1 (MQ=60)
                                         CGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATT                                                      >  SRR4302540.3177919/1‑94 (MQ=60)
                                         CGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATT                                                      >  SRR4302540.2976543/1‑94 (MQ=60)
                                           ACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGG                                                    <  SRR4302540.3115813/94‑1 (MQ=60)
                                            CATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGC                                                   >  SRR4302540.8738779/1‑94 (MQ=60)
                                            CATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGC                                                   <  SRR4302540.2563746/94‑1 (MQ=60)
                                             ATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGTAAGGGGCCGCAAAGGTCCAGAATTGGCT                                                  >  SRR4302540.9059338/1‑94 (MQ=60)
                                             ATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCT                                                  >  SRR4302540.4067457/1‑94 (MQ=60)
                                             ATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCT                                                  >  SRR4302540.8428977/1‑94 (MQ=60)
                                             ATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCT                                                  <  SRR4302540.9689492/94‑1 (MQ=60)
                                              TTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTA                                                 >  SRR4302540.3042381/1‑94 (MQ=60)
                                               TGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCA                                                <  SRR4302540.7313239/94‑1 (MQ=60)
                                               TGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCGGCAAAGGTCCAGAATTGGCTCA                                                <  SRR4302540.5656724/94‑1 (MQ=60)
                                                GTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAA                                               >  SRR4302540.489847/1‑94 (MQ=60)
                                                GTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAA                                               >  SRR4302540.1250521/1‑94 (MQ=60)
                                                GTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAA                                               >  SRR4302540.3981963/1‑94 (MQ=60)
                                                GTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAA                                               >  SRR4302540.9397714/1‑94 (MQ=60)
                                                 TCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAAT                                              >  SRR4302540.2772180/1‑94 (MQ=60)
                                                 TCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAAT                                              <  SRR4302540.2021308/94‑1 (MQ=60)
                                                  CACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATT                                             <  SRR4302540.947903/94‑1 (MQ=60)
                                                  CACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATT                                             >  SRR4302540.8000754/1‑94 (MQ=60)
                                                   ACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTT                                            <  SRR4302540.7031233/94‑1 (MQ=60)
                                                   ACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTT                                            >  SRR4302540.1713929/1‑94 (MQ=60)
                                                    CGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTC                                           >  SRR4302540.10028808/1‑94 (MQ=60)
                                                    CGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTC                                           >  SRR4302540.6637653/1‑94 (MQ=60)
                                                    CGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGGGGGGGGTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTC                                           >  SRR4302540.6637654/1‑94 (MQ=60)
                                                     GTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCC                                          >  SRR4302540.9914362/1‑94 (MQ=60)
                                                      TCCCATTTACATTCTCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCC                                         <  SRR4302540.6528749/94‑1 (MQ=60)
                                                      TCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCC                                         >  SRR4302540.5230495/1‑94 (MQ=60)
                                                      TCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCC                                         >  SRR4302540.5562992/1‑94 (MQ=60)
                                                        CCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTC                                       >  SRR4302540.6161558/1‑94 (MQ=60)
                                                        CCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTC                                       >  SRR4302540.3560876/1‑94 (MQ=60)
                                                         CATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCC                                      >  SRR4302540.7693159/1‑94 (MQ=60)
                                                         CATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCC                                      >  SRR4302540.5537829/1‑94 (MQ=60)
                                                         CATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTAGTTTTCGTTGTGGGAGGGGCCGCAACGGTCCAGGATTGGCTCAATCTCCCTCA                                      >  SRR4302540.5537826/1‑94 (MQ=60)
                                                         CATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGGTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCcgc                                      >  SRR4302540.2050387/1‑91 (MQ=60)
                                                             TACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCC                                  <  SRR4302540.9757761/94‑1 (MQ=60)
                                                              ACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCA                                 <  SRR4302540.3088145/94‑1 (MQ=60)
                                                               CATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAG                                <  SRR4302540.7997403/94‑1 (MQ=60)
                                                               CATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAG                                <  SRR4302540.3518007/94‑1 (MQ=60)
                                                               CATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAG                                >  SRR4302540.3610135/1‑94 (MQ=60)
                                                                ATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTTCCTCCATCCAGG                               >  SRR4302540.8071279/1‑94 (MQ=60)
                                                                ATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGG                               >  SRR4302540.9813941/1‑94 (MQ=60)
                                                                ATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGG                               <  SRR4302540.7468921/94‑1 (MQ=60)
                                                                ATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGG                               <  SRR4302540.3808047/94‑1 (MQ=60)
                                                                ATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGG                               <  SRR4302540.4953005/94‑1 (MQ=60)
                                                                ATTCGCGTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGG                               <  SRR4302540.690503/94‑1 (MQ=60)
                                                                 TTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGA                              <  SRR4302540.3887268/94‑1 (MQ=60)
                                                                  TCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAA                             <  SRR4302540.5909484/94‑1 (MQ=60)
                                                                  TCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAA                             <  SRR4302540.9737062/94‑1 (MQ=60)
                                                                  TCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAA                             <  SRR4302540.1650503/94‑1 (MQ=60)
                                                                  TCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAA                             <  SRR4302540.8559759/94‑1 (MQ=60)
                                                                  TCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAA                             <  SRR4302540.4021264/94‑1 (MQ=60)
                                                                    GCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGG                           >  SRR4302540.2119206/1‑94 (MQ=60)
                                                                    GCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGG                           >  SRR4302540.7721695/1‑94 (MQ=60)
                                                                     CTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGT                          >  SRR4302540.8519613/1‑94 (MQ=60)
                                                                     CTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGG                          <  SRR4302540.1945699/94‑1 (MQ=60)
                                                                     CTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGG                          <  SRR4302540.7325228/94‑1 (MQ=60)
                                                                     CTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGG                          <  SRR4302540.2714800/94‑1 (MQ=60)
                                                                      TTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGT                         <  SRR4302540.6341030/94‑1 (MQ=60)
                                                                      TTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGT                         <  SRR4302540.867471/94‑1 (MQ=60)
                                                                      TTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGT                         >  SRR4302540.6321334/1‑94 (MQ=60)
                                                                      TTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGT                         <  SRR4302540.3640532/94‑1 (MQ=60)
                                                                       TGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTT                        >  SRR4302540.5852029/1‑94 (MQ=60)
                                                                       TGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTT                        <  SRR4302540.7606618/94‑1 (MQ=60)
                                                                       TGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTT                        <  SRR4302540.7606617/94‑1 (MQ=60)
                                                                       TGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTT                        >  SRR4302540.4465164/1‑94 (MQ=60)
                                                                        GCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTT                       <  SRR4302540.3775698/94‑1 (MQ=60)
                                                                         CTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGACTTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTG                      >  SRR4302540.5101105/1‑94 (MQ=60)
                                                                         CTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTG                      >  SRR4302540.3754954/1‑94 (MQ=60)
                                                                         CTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTG                      >  SRR4302540.7820451/1‑94 (MQ=60)
                                                                         CTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTG                      <  SRR4302540.6352986/94‑1 (MQ=60)
                                                                         CTGCGTGCGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTG                      >  SRR4302540.2470520/1‑94 (MQ=60)
                                                                          TGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGT                     <  SRR4302540.6009281/94‑1 (MQ=60)
                                                                           GCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTA                    >  SRR4302540.4102400/1‑94 (MQ=60)
                                                                            CGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAA                   >  SRR4302540.4324167/1‑94 (MQ=60)
                                                                             GTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAAC                  >  SRR4302540.7347873/1‑94 (MQ=60)
                                                                             GTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAAC                  >  SRR4302540.1931998/1‑94 (MQ=60)
                                                                             GTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAAC                  <  SRR4302540.7343985/94‑1 (MQ=60)
                                                                              TGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACA                 <  SRR4302540.187785/94‑1 (MQ=60)
                                                                              GGTGCAGGGAGGTGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACA                 <  SRR4302540.8506013/94‑1 (MQ=60)
                                                                               GTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAG                >  SRR4302540.6413287/1‑94 (MQ=60)
                                                                               GTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAG                >  SRR4302540.8515916/1‑94 (MQ=60)
                                                                               GTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAG                <  SRR4302540.7536186/94‑1 (MQ=60)
                                                                               GTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAG                <  SRR4302540.1831252/94‑1 (MQ=60)
                                                                                TGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGA               >  SRR4302540.9861993/1‑94 (MQ=60)
                                                                                TGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGA               <  SRR4302540.2902321/94‑1 (MQ=60)
                                                                                 GCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAGGGGTTTGTAACAGAA              >  SRR4302540.113206/1‑94 (MQ=60)
                                                                                 GCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAA              <  SRR4302540.6797677/94‑1 (MQ=60)
                                                                                 GCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAA              <  SRR4302540.9003862/94‑1 (MQ=60)
                                                                                 GCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAAGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAA              <  SRR4302540.5904181/94‑1 (MQ=60)
                                                                                  CAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAAT             <  SRR4302540.4211572/94‑1 (MQ=60)
                                                                                   AGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATA            <  SRR4302540.5782043/94‑1 (MQ=60)
                                                                                    GGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATAC           <  SRR4302540.9359418/94‑1 (MQ=60)
                                                                                     GGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACC          >  SRR4302540.2585000/1‑94 (MQ=60)
                                                                                     GGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACC          <  SRR4302540.4716152/94‑1 (MQ=60)
                                                                                      GAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCA         >  SRR4302540.4160228/1‑94 (MQ=60)
                                                                                      GAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCA         <  SRR4302540.1089505/94‑1 (MQ=60)
                                                                                       AGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCAT        >  SRR4302540.148956/1‑94 (MQ=60)
                                                                                       AGTGGGGGTTATTGTTCTTTGCGTAAGGCGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCAT        <  SRR4302540.2870744/94‑1 (MQ=60)
                                                                                        GTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATA       >  SRR4302540.8533609/1‑94 (MQ=60)
                                                                                        GTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATA       <  SRR4302540.9830449/94‑1 (MQ=60)
                                                                                        GTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATA       >  SRR4302540.4072465/1‑94 (MQ=60)
                                                                                          GGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAAT     <  SRR4302540.8265171/94‑1 (MQ=60)
                                                                                           GGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATG    >  SRR4302540.7157921/1‑94 (MQ=60)
                                                                                           GGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATG    >  SRR4302540.338278/1‑94 (MQ=60)
                                                                                           GGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATG    >  SRR4302540.5468404/1‑94 (MQ=60)
                                                                                           GGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATG    >  SRR4302540.3725026/1‑94 (MQ=60)
                                                                                           GGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATG    >  SRR4302540.3151437/1‑94 (MQ=60)
                                                                                            GGGTTATTTTTCTTTGCGGAAGGGGCCGCACAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGTGTTTGTAACAGAATACCATAATGT   <  SRR4302540.1158076/94‑1 (MQ=60)
                                                                                            GGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGT   >  SRR4302540.732473/1‑94 (MQ=60)
                                                                                            GGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGT   >  SRR4302540.1159842/1‑94 (MQ=60)
                                                                                            GGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGT   <  SRR4302540.8382149/94‑1 (MQ=60)
                                                                                            GGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGT   <  SRR4302540.2551353/94‑1 (MQ=60)
                                                                                            GGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGT   >  SRR4302540.8760781/1‑94 (MQ=60)
                                                                                             |                                                                                             
AGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTT  >  NC_000913/4083378‑4083564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑