Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,083,471 A→G intergenic (‑69/‑125) fdoG ← / → fdhD formate dehydrogenase‑O, major subunit/affects formate dehydrogenase‑N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,083,4710AG99.6% 769.8 / ‑5.9 239intergenic (‑69/‑125)fdoG/fdhDformate dehydrogenase‑O, major subunit/affects formate dehydrogenase‑N
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (127/111);  minor base T (0/1);  total (127/112)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 265 total aligned reads displayed.

CTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAA  >  NC_000913/4083382‑4083560
                                                                                         |                                                                                         
cTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGtt                                                                                        >  2:5405779/1‑93 (MQ=255)
cTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGtt                                                                                        >  1:2994376/1‑93 (MQ=255)
cTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGtt                                                                                        <  1:2686997/93‑1 (MQ=255)
 tGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTa                                                                                       >  2:5241347/1‑93 (MQ=255)
  gCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTAt                                                                                      >  2:4034944/1‑93 (MQ=255)
   ccTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTAtt                                                                                     >  1:5627671/1‑93 (MQ=255)
   ccTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTAtt                                                                                     <  1:3900063/93‑1 (MQ=255)
   ccTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGATAtt                                                                                     >  1:1565512/1‑93 (MQ=255)
      tCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTAttttt                                                                                  <  2:4629707/93‑1 (MQ=255)
       ctgctgACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGGTAtttttt                                                                                 >  1:3540773/1‑92 (MQ=255)
        tgctgACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCt                                                                                >  1:1159637/1‑93 (MQ=255)
        tgctgACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCt                                                                                <  1:3600638/93‑1 (MQ=255)
           tgACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTg                                                                             <  2:1315966/93‑1 (MQ=255)
            gACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGc                                                                            <  1:2489977/93‑1 (MQ=255)
            gACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGc                                                                            >  2:2250367/1‑93 (MQ=255)
            gACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGc                                                                            >  1:1208845/1‑93 (MQ=255)
            gACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGc                                                                            <  2:5806560/93‑1 (MQ=255)
             aCCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCg                                                                           >  2:297295/1‑93 (MQ=255)
             aCCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCg                                                                           <  1:2623795/93‑1 (MQ=255)
              ccTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCgg                                                                          <  2:1713280/93‑1 (MQ=255)
              ccTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCgg                                                                          >  2:4894633/1‑93 (MQ=255)
               cTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGa                                                                         >  1:5006124/1‑93 (MQ=255)
               cTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGa                                                                         <  2:2078109/93‑1 (MQ=255)
                tGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGaa                                                                        <  2:3033703/93‑1 (MQ=255)
                tGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGCGGTTATTTTTCTTTGCGGaa                                                                        <  2:3588966/93‑1 (MQ=255)
                 gCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAg                                                                       >  1:38913/1‑93 (MQ=255)
                  cATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAgg                                                                      <  1:5958023/93‑1 (MQ=255)
                   aTGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAggg                                                                     <  2:5905527/93‑1 (MQ=255)
                   aTGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAggg                                                                     <  2:96040/93‑1 (MQ=255)
                    tGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAgggg                                                                    >  1:150063/1‑93 (MQ=255)
                    tGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAgggg                                                                    >  1:4499753/1‑93 (MQ=255)
                     ggATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGc                                                                   <  2:2510148/93‑1 (MQ=255)
                      gATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGcc                                                                  <  1:3241060/93‑1 (MQ=255)
                       aTTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCg                                                                 >  1:832324/1‑93 (MQ=255)
                        ttGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGc                                                                <  2:4068647/93‑1 (MQ=255)
                        ttGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGc                                                                >  2:135325/1‑93 (MQ=255)
                        ttGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGc                                                                >  1:5355351/1‑93 (MQ=255)
                          gCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCaa                                                              <  1:1906004/93‑1 (MQ=255)
                            tCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAg                                                            >  2:5166125/1‑93 (MQ=255)
                              cTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGt                                                          >  2:3746081/1‑93 (MQ=255)
                              cTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGt                                                          >  2:3415888/1‑93 (MQ=255)
                              cTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGt                                                          >  2:3881511/1‑93 (MQ=255)
                              cTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGt                                                          <  1:3022132/93‑1 (MQ=255)
                               ttGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTc                                                         >  1:6146800/1‑93 (MQ=255)
                               ttGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTc                                                         >  2:351950/1‑93 (MQ=255)
                               ttGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTc                                                         <  2:5196647/93‑1 (MQ=255)
                                tGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTcc                                                        <  1:4773018/93‑1 (MQ=255)
                                 gTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCa                                                       <  2:3092815/93‑1 (MQ=255)
                                  tttCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAg                                                      >  1:4163298/1‑93 (MQ=255)
                                    tCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGaa                                                    >  1:6185006/1‑93 (MQ=255)
                                    tCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGaa                                                    <  1:2103538/93‑1 (MQ=255)
                                    tCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGaa                                                    >  2:5025878/1‑93 (MQ=255)
                                    tCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGaa                                                    <  1:2191399/93‑1 (MQ=255)
                                     cGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAAt                                                   <  2:1801482/93‑1 (MQ=255)
                                     cGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAAt                                                   <  1:722990/93‑1 (MQ=255)
                                      gACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAAtt                                                  >  2:4501564/1‑93 (MQ=255)
                                       aCATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTg                                                 >  2:6042221/1‑93 (MQ=255)
                                       aCATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTg                                                 >  2:2733973/1‑93 (MQ=255)
                                         aTTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGc                                               >  2:877080/1‑93 (MQ=255)
                                            gTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCa                                            <  1:2255949/93‑1 (MQ=255)
                                            gTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCa                                            >  1:4690682/1‑93 (MQ=255)
                                             tCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCaa                                           >  1:2798552/1‑93 (MQ=255)
                                               aCGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAAtt                                         >  2:3165924/1‑93 (MQ=255)
                                                  tCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTcc                                      <  1:461120/93‑1 (MQ=255)
                                                  tCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTcc                                      >  1:3993588/1‑93 (MQ=255)
                                                   cccATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTccc                                     <  1:3086988/93‑1 (MQ=255)
                                                    ccATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCt                                    >  1:2338034/1‑93 (MQ=255)
                                                      aTTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtcc                                  >  2:677892/1‑93 (MQ=255)
                                                       tttACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtcca                                 >  1:5218650/1‑93 (MQ=255)
                                                       tttACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtcca                                 >  1:5402250/1‑93 (MQ=255)
                                                         tACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCtccatc                               <  2:1214697/93‑1 (MQ=255)
                                                             ttCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAgg                           >  1:926568/1‑93 (MQ=255)
                                                             ttCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGGCCAGAATTGGCTCAATTTCCCTCCATCCAgg                           >  2:1657826/1‑93 (MQ=255)
                                                               cGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGaa                         >  1:1139469/1‑93 (MQ=255)
                                                                gCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAg                        <  2:381071/93‑1 (MQ=255)
                                                                  ttGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAggg                      >  2:1384059/1‑93 (MQ=255)
                                                                    gctgcGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGtt                    <  1:2847967/93‑1 (MQ=255)
                                                                    gctgcGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGtt                    <  1:3324680/93‑1 (MQ=255)
                                                                    gctgcGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGtt                    <  2:4979311/93‑1 (MQ=255)
                                                                    gctgcGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGtt                    >  2:6185119/1‑93 (MQ=255)
                                                                    gctgcGTGTGCAGGGAGAGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGACCAGCATTGGCTCAATTTCCCTCCATCCAGGAAGGGtt                    >  2:5319866/1‑93 (MQ=255)
                                                                     ctgcGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGttt                   <  1:106071/93‑1 (MQ=255)
                                                                      tgcGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTg                  >  1:4085436/1‑93 (MQ=255)
                                                                      tgcGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTg                  >  1:1647150/1‑93 (MQ=255)
                                                                      tgcGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTg                  <  1:1009427/93‑1 (MQ=255)
                                                                       gcGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGGTTGt                 >  2:1540168/1‑93 (MQ=255)
                                                                        cGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTa                <  1:4430580/93‑1 (MQ=255)
                                                                        cGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTa                <  2:1936169/93‑1 (MQ=255)
                                                                          tgtgCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAAc              >  1:4079239/1‑93 (MQ=255)
                                                                           gtgCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACa             <  2:6142026/93‑1 (MQ=255)
                                                                             gCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGa           <  1:2897191/93‑1 (MQ=255)
                                                                              cAGCGAGTGGCATTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGaa          <  1:3677628/93‑1 (MQ=255)
                                                                               aGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAAt         >  2:5715135/1‑93 (MQ=255)
                                                                                gggagtgggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATa        <  1:5720993/93‑1 (MQ=255)
                                                                                 ggagtgggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATAc       <  2:259564/93‑1 (MQ=255)
                                                                                   agtgggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTTGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCa     >  2:6012115/1‑93 (MQ=255)
                                                                                   agtgggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCa     <  1:512038/93‑1 (MQ=255)
                                                                                    gtgggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCAt    >  1:1414221/1‑93 (MQ=255)
                                                                                     tgggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATa   <  1:5547658/93‑1 (MQ=255)
                                                                                      gggggtTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATaa  <  2:4240728/93‑1 (MQ=255)
                                                                                         |                                                                                         
CTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAA  >  NC_000913/4083382‑4083560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTT  >  NC_000913/4083378‑4083564
                                                                                             |                                                                                             
AGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGG                                                                                               >  SRR4302533.5593221/1‑94 (MQ=60)
 GAACTTCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGG                                                                                              <  SRR4302533.4232814/94‑1 (MQ=60)
 GAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGG                                                                                              <  SRR4302533.895377/94‑1 (MQ=60)
 GAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGG                                                                                              <  SRR4302533.306463/94‑1 (MQ=60)
   ACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTT                                                                                            >  SRR4302533.3012684/1‑94 (MQ=60)
    CTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTA                                                                                           <  SRR4302533.2703470/94‑1 (MQ=60)
     TGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTAT                                                                                          >  SRR4302533.4109297/1‑94 (MQ=60)
     TGCCTTCTGCTCACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTAT                                                                                          <  SRR4302533.5481184/94‑1 (MQ=60)
      GCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATT                                                                                         >  SRR4302533.5661696/1‑94 (MQ=60)
      GCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATT                                                                                         <  SRR4302533.6255949/94‑1 (MQ=60)
      GCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGATATT                                                                                         >  SRR4302533.1574940/1‑94 (MQ=60)
       CCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTT                                                                                        >  SRR4302533.4044841/1‑94 (MQ=60)
       CCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTT                                                                                        <  SRR4302533.3923867/94‑1 (MQ=60)
        CTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTT                                                                                       >  SRR4302533.4602325/1‑94 (MQ=60)
         TTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTT                                                                                      >  SRR4302533.4555012/1‑94 (MQ=60)
          TCTGCTGACCTGCATGGTTTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTT                                                                                     >  SRR4302533.1147081/1‑94 (MQ=60)
          TCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGGTATTTTTT                                                                                     >  SRR4302533.3562589/1‑94 (MQ=60)
           CTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCT                                                                                    >  SRR4302533.1166596/1‑94 (MQ=60)
            TGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTT                                                                                   >  SRR4302533.2996389/1‑94 (MQ=60)
            TGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTT                                                                                   <  SRR4302533.3622793/94‑1 (MQ=60)
              CTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTG                                                                                 <  SRR4302533.3966465/94‑1 (MQ=60)
              CTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTG                                                                                 >  SRR4302533.1050569/1‑94 (MQ=60)
              CTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTG                                                                                 <  SRR4302533.4184321/94‑1 (MQ=60)
               TGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGC                                                                                >  SRR4302533.1216105/1‑94 (MQ=60)
                GACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCG                                                                               <  SRR4302533.2505312/94‑1 (MQ=60)
                 ACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGG                                                                              <  SRR4302533.2639900/94‑1 (MQ=60)
                  CCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGA                                                                             >  SRR4302533.5036460/1‑94 (MQ=60)
                   CTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAA                                                                            <  SRR4302533.6178254/94‑1 (MQ=60)
                    TGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAG                                                                           >  SRR4302533.39170/1‑94 (MQ=60)
                     GCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGG                                                                          >  SRR4302533.2701289/1‑94 (MQ=60)
                      CATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGG                                                                         <  SRR4302533.5994060/94‑1 (MQ=60)
                       ATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGG                                                                        >  SRR4302533.1645184/1‑94 (MQ=60)
                       ATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGG                                                                        >  SRR4302533.151027/1‑94 (MQ=60)
                       ATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGG                                                                        >  SRR4302533.4527002/1‑94 (MQ=60)
                         GGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCC                                                                      <  SRR4302533.3344982/94‑1 (MQ=60)
                          GATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCG                                                                     <  SRR4302533.3261034/94‑1 (MQ=60)
                          GATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCG                                                                     <  SRR4302533.4665029/94‑1 (MQ=60)
                          GATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCG                                                                     >  SRR4302533.837201/1‑94 (MQ=60)
                           ATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGC                                                                    >  SRR4302533.1274530/1‑94 (MQ=60)
                           ATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGC                                                                    >  SRR4302533.5387497/1‑94 (MQ=60)
                             TGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAA                                                                  >  SRR4302533.410270/1‑94 (MQ=60)
                              GCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAA                                                                 <  SRR4302533.1917630/94‑1 (MQ=60)
                               CTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAG                                                                >  SRR4302533.3057290/1‑94 (MQ=60)
                                TCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGG                                                               >  SRR4302533.863111/1‑94 (MQ=60)
                                 CCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGT                                                              <  SRR4302533.5615612/94‑1 (MQ=60)
                                 CCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGC                                                              >  SRR4302533.4396697/1‑94 (MQ=60)
                                 CCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCAGCAAAGGC                                                              >  SRR4302533.1111864/1‑94 (MQ=60)
                                  CTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTC                                                             <  SRR4302533.2720696/94‑1 (MQ=60)
                                  CTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTC                                                             >  SRR4302533.5140606/1‑94 (MQ=60)
                                  CTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTC                                                             >  SRR4302533.6183805/1‑94 (MQ=60)
                                  CTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTC                                                             <  SRR4302533.3040621/94‑1 (MQ=60)
                                    TGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCA                                                           <  SRR4302533.4801942/94‑1 (MQ=60)
                                    TGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCA                                                           <  SRR4302533.2985837/94‑1 (MQ=60)
                                     GTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAG                                                          >  SRR4302533.4188522/1‑94 (MQ=60)
                                      TTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGA                                                         >  SRR4302533.2319271/1‑94 (MQ=60)
                                      TTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGA                                                         <  SRR4302533.3271644/94‑1 (MQ=60)
                                      TTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGA                                                         >  SRR4302533.790399/1‑94 (MQ=60)
                                       TTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAA                                                        >  SRR4302533.6222138/1‑94 (MQ=60)
                                       TTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAA                                                        <  SRR4302533.6272710/94‑1 (MQ=60)
                                        TCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAAT                                                       <  SRR4302533.2116507/94‑1 (MQ=60)
                                        TCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAAT                                                       <  SRR4302533.2204903/94‑1 (MQ=60)
                                         CGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATT                                                      <  SRR4302533.727266/94‑1 (MQ=60)
                                         CGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATT                                                      <  SRR4302533.3697171/94‑1 (MQ=60)
                                         CGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATT                                                      <  SRR4302533.3697172/94‑1 (MQ=60)
                                          GACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTG                                                     <  SRR4302533.2832981/94‑1 (MQ=60)
                                          GACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTG                                                     <  SRR4302533.4687497/94‑1 (MQ=60)
                                          GACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTG                                                     >  SRR4302533.392330/1‑94 (MQ=60)
                                          GACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTG                                                     >  SRR4302533.2187114/1‑94 (MQ=60)
                                          GACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCAAGAATTG                                                     <  SRR4302533.317523/94‑1 (MQ=60)
                                          GACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGGTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTG                                                     >  SRR4302533.1553943/1‑94 (MQ=60)
                                            CATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGC                                                   >  SRR4302533.2681603/1‑94 (MQ=60)
                                            CATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGC                                                   >  SRR4302533.4931819/1‑94 (MQ=60)
                                            CATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGC                                                   >  SRR4302533.3812912/1‑94 (MQ=60)
                                               TGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCA                                                >  SRR4302533.4719115/1‑94 (MQ=60)
                                                GTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAA                                               <  SRR4302533.2269906/94‑1 (MQ=60)
                                                GTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAA                                               >  SRR4302533.2815765/1‑94 (MQ=60)
                                                GTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAA                                               >  SRR4302533.1093627/1‑94 (MQ=60)
                                                  CACGTCCCATTTACATTCGCTTGCTGCGTGGGCAGGGAGGGGGGGTTATTTTggcttgcgaccgtggccccaaaggtccagaagtggctcaagt                                             >  SRR4302533.1697030/1‑52 (MQ=49)
                                                   ACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTT                                            >  SRR4302533.5262106/1‑94 (MQ=60)
                                                     GTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCC                                          >  SRR4302533.2142553/1‑94 (MQ=60)
                                                     GTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCC                                          >  SRR4302533.4017906/1‑94 (MQ=60)
                                                     GTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCC                                          >  SRR4302533.1937938/1‑94 (MQ=60)
                                                     GTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCC                                          >  SRR4302533.2481272/1‑94 (MQ=60)
                                                     GTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCC                                          >  SRR4302533.5725610/1‑94 (MQ=60)
                                                      TCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCC                                         <  SRR4302533.6208808/94‑1 (MQ=60)
                                                      TCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCC                                         <  SRR4302533.463871/94‑1 (MQ=60)
                                                      TCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCC                                         <  SRR4302533.186872/94‑1 (MQ=60)
                                                       CCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGTGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCT                                        <  SRR4302533.2873669/94‑1 (MQ=60)
                                                       CCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCT                                        <  SRR4302533.3105969/94‑1 (MQ=60)
                                                       CCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCT                                        >  SRR4302533.2352506/1‑94 (MQ=60)
                                                         CATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCC                                      >  SRR4302533.1374439/1‑94 (MQ=60)
                                                         CATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCC                                      <  SRR4302533.3498846/94‑1 (MQ=60)
                                                          ATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCA                                     >  SRR4302533.5250061/1‑94 (MQ=60)
                                                          ATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCA                                     >  SRR4302533.5434713/1‑94 (MQ=60)
                                                          ATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCA                                     >  SRR4302533.3610647/1‑94 (MQ=60)
                                                          ATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCA                                     <  SRR4302533.5634079/94‑1 (MQ=60)
                                                           TTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCACAGGTCCAGAATTGGCTCAATTTCCCTCCAT                                    >  SRR4302533.1210506/1‑94 (MQ=60)
                                                           TTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCAT                                    <  SRR4302533.5949602/94‑1 (MQ=60)
                                                           TTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCAT                                    >  SRR4302533.222922/1‑94 (MQ=60)
                                                            TTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATC                                   >  SRR4302533.829820/1‑94 (MQ=60)
                                                            TTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATC                                   >  SRR4302533.5416912/1‑94 (MQ=60)
                                                            TTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATC                                   >  SRR4302533.3104037/1‑94 (MQ=60)
                                                            TTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATC                                   <  SRR4302533.6041514/94‑1 (MQ=60)
                                                            TTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATC                                   <  SRR4302533.4322479/94‑1 (MQ=60)
                                                              ACATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCA                                 <  SRR4302533.3021225/94‑1 (MQ=60)
                                                               CATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAG                                >  SRR4302533.2348734/1‑94 (MQ=60)
                                                                ATTCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGG                               >  SRR4302533.932003/1‑94 (MQ=60)
                                                                  TCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAA                             <  SRR4302533.1426225/94‑1 (MQ=60)
                                                                  TCGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAA                             >  SRR4302533.1146250/1‑94 (MQ=60)
                                                                   CGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAG                            <  SRR4302533.4977010/94‑1 (MQ=60)
                                                                   CGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAG                            <  SRR4302533.2037897/94‑1 (MQ=60)
                                                                   CGCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAG                            <  SRR4302533.5303553/94‑1 (MQ=60)
                                                                    GCTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAACGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGG                           >  SRR4302533.2459772/1‑94 (MQ=60)
                                                                     CTTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGG                          >  SRR4302533.5569537/1‑94 (MQ=60)
                                                                      TTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGT                         <  SRR4302533.4721117/94‑1 (MQ=60)
                                                                      TTGCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGT                         <  SRR4302533.5178104/94‑1 (MQ=60)
                                                                        GCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTT                       <  SRR4302533.3345219/94‑1 (MQ=60)
                                                                        GCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTT                       <  SRR4302533.2887964/94‑1 (MQ=60)
                                                                        GCTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTT                       <  SRR4302533.2865492/94‑1 (MQ=60)
                                                                         CTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTG                      >  SRR4302533.2539707/1‑94 (MQ=60)
                                                                         CTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTG                      >  SRR4302533.4110306/1‑94 (MQ=60)
                                                                         CTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTG                      <  SRR4302533.106730/94‑1 (MQ=60)
                                                                         CTGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTG                      >  SRR4302533.1657127/1‑94 (MQ=60)
                                                                          TGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGT                     <  SRR4302533.1015332/94‑1 (MQ=60)
                                                                          TGCGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGT                     >  SRR4302533.3489296/1‑94 (MQ=60)
                                                                            CGTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAA                   <  SRR4302533.4457363/94‑1 (MQ=60)
                                                                             GTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAAC                  >  SRR4302533.383864/1‑94 (MQ=60)
                                                                             GTGTGCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAAC                  >  SRR4302533.4104071/1‑94 (MQ=60)
                                                                                 GCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAA              <  SRR4302533.4167566/94‑1 (MQ=60)
                                                                                 GCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAA              <  SRR4302533.1598195/94‑1 (MQ=60)
                                                                                 GCAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAA              <  SRR4302533.2915002/94‑1 (MQ=60)
                                                                                  CAGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGGTTGTAACAGAAT             >  SRR4302533.2753615/1‑94 (MQ=60)
                                                                                  CAGCGAGTGGGCGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAAT             <  SRR4302533.151772/94‑1 (MQ=60)
                                                                                  CAGCGAGTGGCATTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAAT             <  SRR4302533.3700232/94‑1 (MQ=60)
                                                                                   AGGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGTGGCAAAGGTCCAGAATTGGCTCAATTTCCCCCCATCCAGGAAGGGTTTGTAACAGAATA            <  SRR4302533.2021518/94‑1 (MQ=60)
                                                                                    GGGAGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATAC           <  SRR4302533.5755535/94‑1 (MQ=60)
                                                                                       AGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCAT        >  SRR4302533.4008371/1‑94 (MQ=60)
                                                                                       AGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCAT        >  SRR4302533.1422774/1‑94 (MQ=60)
                                                                                       AGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCAT        <  SRR4302533.1195085/94‑1 (MQ=60)
                                                                                       AGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCAT        <  SRR4302533.515081/94‑1 (MQ=60)
                                                                                       AGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCAT        <  SRR4302533.508132/94‑1 (MQ=60)
                                                                                       AGTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTACATCCAGGAAGGGTTTGTAACAGAATACCAT        >  SRR4302533.5946882/1‑94 (MQ=60)
                                                                                        GTGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGTATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATA       >  SRR4302533.4465643/1‑94 (MQ=60)
                                                                                         TGGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAA      <  SRR4302533.5581162/94‑1 (MQ=60)
                                                                                          GGGGGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAAT     >  SRR4302533.3779961/1‑94 (MQ=60)
                                                                                             GGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTT  <  SRR4302533.5751501/94‑1 (MQ=60)
                                                                                             |                                                                                             
AGAACTGCCTTCTGCTGACCTGCATGGATTGCTCCTTGTTTCGACATTGTCACGTCCCATTTACATTCGCTTGCTGCGTGTGCAGGGAGTGGGAGTTATTTTTCTTTGCGGAAGGGGCCGCAAAGGTCCAGAATTGGCTCAATTTCCCTCCATCCAGGAAGGGTTTGTAACAGAATACCATAATGTT  >  NC_000913/4083378‑4083564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑