Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,091,351 C→T G168D (GGC→GAC)  rhaD ← rhamnulose‑phosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,091,3510CT99.7% 1288.4 / NA 354G168D (GGC→GAC) rhaDrhamnulose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (142/210);  total (143/211)
Fisher's exact test for biased strand distribution p-value = 4.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 367 total aligned reads displayed.

GCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATC  >  NC_000913/4091261‑4091440
                                                                                          |                                                                                         
  ggTCGCCTGGCCTATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTc                                                                                       <  1:3256849/93‑1 (MQ=255)
   gTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTcc                                                                                      >  1:5753579/1‑93 (MQ=255)
      cccTGGCCCATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCcac                                                                                   <  1:2700147/92‑1 (MQ=255)
       gcTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCcaca                                                                                  <  1:4498780/92‑1 (MQ=255)
         tGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGt                                                                                >  2:9809085/1‑93 (MQ=255)
         tGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGt                                                                                <  2:7324393/93‑1 (MQ=255)
         tGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGt                                                                                >  1:6487593/1‑93 (MQ=255)
         tGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGt                                                                                >  1:5590660/1‑93 (MQ=255)
         tGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGt                                                                                >  1:1854512/1‑93 (MQ=255)
          ggCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGtt                                                                               <  1:9096760/93‑1 (MQ=255)
            ccGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTgg                                                                             >  2:2604060/1‑93 (MQ=255)
            ccGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTgg                                                                             <  2:8513671/93‑1 (MQ=255)
              gATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCg                                                                           >  1:8854502/1‑93 (MQ=255)
              gATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCg                                                                           >  1:759201/1‑93 (MQ=255)
              gATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCg                                                                           <  1:5284715/93‑1 (MQ=255)
              gATTTAGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCg                                                                           >  2:5011292/1‑93 (MQ=255)
              gATGTCGTCCGTGCCGGGCTCGATCCACGGCAAATTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCg                                                                           <  1:1691593/93‑1 (MQ=255)
               aTTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGa                                                                          <  1:1060796/93‑1 (MQ=255)
               aTTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGa                                                                          >  1:4139800/1‑93 (MQ=255)
               aTTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGa                                                                          <  2:8845914/93‑1 (MQ=255)
               aTTCCGTCCGTGCAGCGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGa                                                                          <  2:9017532/93‑1 (MQ=255)
                   cGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGa                                                                      >  2:1758786/1‑93 (MQ=255)
                   cGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGa                                                                      <  2:4515306/93‑1 (MQ=255)
                     tCCGTCCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAg                                                                    <  2:3998647/93‑1 (MQ=255)
                        gTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGTAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGAcc                                                                 <  1:7145639/93‑1 (MQ=255)
                          gCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGTGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcgc                                                               <  1:533943/93‑1 (MQ=255)
                          gCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcgc                                                               >  2:2976141/1‑93 (MQ=255)
                           ccGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcgcg                                                              <  1:3735257/93‑1 (MQ=255)
                            cGGGCCCCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCgg                                                             <  2:6322075/93‑1 (MQ=255)
                            cGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATCCCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCgg                                                             <  1:6208614/93‑1 (MQ=255)
                            cGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCgg                                                             <  2:4762533/93‑1 (MQ=255)
                              ggCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgtg                                                           >  2:1451905/1‑93 (MQ=255)
                               gCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgtgt                                                          <  2:1730615/93‑1 (MQ=255)
                               gCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgtgt                                                          <  2:4240781/93‑1 (MQ=255)
                                cACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGCCCGCGgtgtg                                                         >  1:8340409/1‑92 (MQ=255)
                                cACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTc                                                         >  2:10031880/1‑93 (MQ=255)
                                   cATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGAc                                                                  >  2:559068/1‑81 (MQ=255)
                                    aTCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGttt                                                     <  2:4096643/93‑1 (MQ=255)
                                     tCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGtttt                                                    >  2:6951010/1‑93 (MQ=255)
                                     tCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGCTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCgagtt                                                    >  2:5332723/1‑89 (MQ=255)
                                      ccACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTc                                                   <  1:3291852/93‑1 (MQ=255)
                                      ccACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTc                                                   <  1:2352584/93‑1 (MQ=255)
                                       cACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCa                                                  >  1:7863197/1‑93 (MQ=255)
                                        ccGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCaa                                                 <  1:8471042/92‑1 (MQ=255)
                                         cGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAg                                                >  2:9380575/1‑93 (MQ=255)
                                          ggCAAAATGCCACCGCCATCCGGGAATCCCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGt                                               <  2:9193939/93‑1 (MQ=255)
                                           gCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTa                                              <  1:134996/93‑1 (MQ=255)
                                            cAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTAc                                             <  1:6660586/93‑1 (MQ=255)
                                             aaaaTGCCAACGCGATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACa                                            <  1:5098566/93‑1 (MQ=255)
                                             aaaaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACa                                            <  1:2444727/93‑1 (MQ=255)
                                              aaaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACAt                                           >  2:7064381/1‑93 (MQ=255)
                                               aaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATa                                          <  1:5227994/93‑1 (MQ=255)
                                               aaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATa                                          <  2:7399751/93‑1 (MQ=255)
                                                aTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAg                                         <  1:491120/93‑1 (MQ=255)
                                                   ccAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTg                                      <  2:7795175/93‑1 (MQ=255)
                                                    cAACGCCATCGGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGa                                     <  1:8282535/93‑1 (MQ=255)
                                                    cAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGa                                     <  2:2789468/93‑1 (MQ=255)
                                                     aaCGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAg                                    >  1:6043785/1‑93 (MQ=255)
                                                      aCGCCATCCGGGAATACCCCCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAgg                                   <  1:2973523/93‑1 (MQ=255)
                                                       cGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAggg                                  <  1:3599737/93‑1 (MQ=255)
                                                         ccATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCg                                <  2:9723262/93‑1 (MQ=255)
                                                         ccATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCg                                <  2:1145253/93‑1 (MQ=255)
                                                          cATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGa                               >  2:5452958/1‑93 (MQ=255)
                                                            tCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATc                             >  2:8330173/1‑93 (MQ=255)
                                                            tCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATc                             <  2:8008433/93‑1 (MQ=255)
                                                            tCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATc                             >  1:9746621/1‑93 (MQ=255)
                                                            tCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATc                             <  1:8901991/93‑1 (MQ=255)
                                                             ccGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCa                            >  2:2418784/1‑93 (MQ=255)
                                                              cGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAg                           <  1:9332801/93‑1 (MQ=255)
                                                                ggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGt                         <  2:235746/93‑1 (MQ=255)
                                                                ggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGt                         <  2:8686545/93‑1 (MQ=255)
                                                                ggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGt                         <  1:4703907/93‑1 (MQ=255)
                                                                ggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGt                         >  2:5883422/1‑93 (MQ=255)
                                                                ggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGt                         <  2:5773318/93‑1 (MQ=255)
                                                                gaaaTACCCCCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGt                         <  2:6761555/91‑1 (MQ=255)
                                                                  aaTACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtg                       >  2:3215716/1‑93 (MQ=255)
                                                                   aTACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtgg                      <  1:4372082/93‑1 (MQ=255)
                                                                    tACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtggt                     >  1:5797240/1‑93 (MQ=255)
                                                                     accaccAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtggtg                    <  1:8556809/93‑1 (MQ=255)
                                                                     accaccAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtggtg                    <  1:2674132/93‑1 (MQ=255)
                                                                        accaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGCTCAGGTTGggggcg                 >  1:1588508/1‑93 (MQ=255)
                                                                         ccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgt                <  1:7274210/93‑1 (MQ=255)
                                                                         ccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgt                <  1:7941730/93‑1 (MQ=255)
                                                                         ccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgt                <  2:6274972/93‑1 (MQ=255)
                                                                          caGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgtg               <  1:6922787/93‑1 (MQ=255)
                                                                          caGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgtg               >  2:7649156/1‑93 (MQ=255)
                                                                          caGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGGTGgtggcgtg               >  1:3861421/1‑93 (MQ=255)
                                                                            gtcacTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgtggc             <  2:3589664/91‑1 (MQ=255)
                                                                              cacTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAg           <  1:9211893/93‑1 (MQ=255)
                                                                               ccTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGt          <  2:2020776/92‑1 (MQ=255)
                                                                               acTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGt          <  2:8569269/93‑1 (MQ=255)
                                                                                cTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTg         >  1:7010690/1‑93 (MQ=255)
                                                                                 tCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGc        >  2:3751182/1‑93 (MQ=255)
                                                                                  cGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGAGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCa       >  1:206882/1‑93 (MQ=255)
                                                                                    gTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATg     >  1:1115500/1‑93 (MQ=255)
                                                                                     tgctgtCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCTGTGCATGa    <  1:8876858/93‑1 (MQ=255)
                                                                                      gctgtCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGCGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGAt   >  1:8081797/1‑93 (MQ=255)
                                                                                      gctgtCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGAt   >  1:8653685/1‑93 (MQ=255)
                                                                                      gctgtCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGAt   <  2:4713304/93‑1 (MQ=255)
                                                                                       ctgtCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATc  >  1:4948715/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATC  >  NC_000913/4091261‑4091440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 213 total aligned reads displayed.
TGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACCC  >  NC_000913/4091258‑4091444
                                                                                             |                                                                                             
TGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAACACCACCAGACACTCGGTGCTGT                                                                                               >  SRR4302540.2358134/1‑94 (MQ=60)
 GTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTC                                                                                              >  SRR4302540.7810311/1‑94 (MQ=60)
  TGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCT                                                                                             >  SRR4302540.6463044/1‑94 (MQ=60)
   GCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTT                                                                                            <  SRR4302540.59377/94‑1 (MQ=60)
     GGTCGCCTGGCCTATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCC                                                                                          <  SRR4302540.3292354/94‑1 (MQ=60)
     GGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGCCACTCGGTGCTGTCTTCC                                                                                          >  SRR4302540.5817331/1‑94 (MQ=60)
     GGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCC                                                                                          >  SRR4302540.5817076/1‑94 (MQ=60)
      GTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCC                                                                                         >  SRR4302540.144479/1‑94 (MQ=60)
      GTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCC                                                                                         >  SRR4302540.175058/1‑94 (MQ=60)
       TCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCA                                                                                        <  SRR4302540.7078420/94‑1 (MQ=60)
       TCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCA                                                                                        <  SRR4302540.8979734/94‑1 (MQ=60)
        CGCCTGGCCGATTTAGTCCGCGCCGGGCTCCATCCACGGCAAAATGCCACCGCCATCCGGTAATACCACCAGACACTCGGTGCTGTCTTCCCAC                                                                                       <  SRR4302540.5369203/94‑1 (MQ=60)
         GCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACA                                                                                      >  SRR4302540.9007668/1‑94 (MQ=60)
         CCCTGGCCCATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACA                                                                                      <  SRR4302540.2729288/94‑1 (MQ=60)
          GCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAG                                                                                     <  SRR4302540.4547550/94‑1 (MQ=60)
           CTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCCCAGT                                                                                    >  SRR4302540.2731920/1‑94 (MQ=60)
           CTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGT                                                                                    <  SRR4302540.7151679/94‑1 (MQ=60)
           CTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGT                                                                                    <  SRR4302540.5021639/94‑1 (MQ=60)
           CTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGT                                                                                    >  SRR4302540.6559740/1‑94 (MQ=60)
           CTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGT                                                                                    >  SRR4302540.5652179/1‑94 (MQ=60)
           CTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGT                                                                                    <  SRR4302540.1831123/94‑1 (MQ=60)
           CTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGT                                                                                    >  SRR4302540.1932463/1‑94 (MQ=60)
           CTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGT                                                                                    >  SRR4302540.3273954/1‑94 (MQ=60)
           CTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGT                                                                                    >  SRR4302540.1874848/1‑94 (MQ=60)
            TGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTT                                                                                   <  SRR4302540.1544489/94‑1 (MQ=60)
             GGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTG                                                                                  <  SRR4302540.2352813/94‑1 (MQ=60)
             GGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTG                                                                                  <  SRR4302540.3760598/94‑1 (MQ=60)
             GGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTG                                                                                  <  SRR4302540.9206061/94‑1 (MQ=60)
             GGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTG                                                                                  <  SRR4302540.9540413/94‑1 (MQ=60)
             GGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTG                                                                                  >  SRR4302540.5171657/1‑94 (MQ=60)
               CCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGC                                                                                >  SRR4302540.8092500/1‑94 (MQ=60)
               CCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGC                                                                                <  SRR4302540.9509655/94‑1 (MQ=60)
               CCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGC                                                                                <  SRR4302540.7187147/94‑1 (MQ=60)
                CGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCG                                                                               >  SRR4302540.767282/1‑94 (MQ=60)
                CGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCG                                                                               <  SRR4302540.756185/94‑1 (MQ=60)
                CGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCG                                                                               >  SRR4302540.8956944/1‑94 (MQ=60)
                 GATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGA                                                                              >  SRR4302540.9239546/1‑94 (MQ=60)
                 GATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGA                                                                              >  SRR4302540.4184598/1‑94 (MQ=60)
                 GATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGA                                                                              <  SRR4302540.5342463/94‑1 (MQ=60)
                 GATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGA                                                                              <  SRR4302540.2599704/94‑1 (MQ=60)
                 GATGTCGTCCGTGCCGGGCTCGATCCACGGCAAATTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGA                                                                              <  SRR4302540.1709938/94‑1 (MQ=60)
                  ATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAG                                                                             >  SRR4302540.9500289/1‑94 (MQ=60)
                  ATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAG                                                                             <  SRR4302540.1071594/94‑1 (MQ=60)
                      CGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAA                                                                         <  SRR4302540.2428275/94‑1 (MQ=60)
                      CGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAA                                                                         <  SRR4302540.4398025/94‑1 (MQ=60)
                      CGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAA                                                                         <  SRR4302540.7049183/94‑1 (MQ=60)
                       GTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAG                                                                        <  SRR4302540.6450379/94‑1 (MQ=60)
                       GTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAG                                                                        <  SRR4302540.1620623/94‑1 (MQ=60)
                       GTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAG                                                                        <  SRR4302540.4640200/94‑1 (MQ=60)
                        TCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGC                                                                       >  SRR4302540.7948606/1‑94 (MQ=60)
                        GCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGA                                                                       <  SRR4302540.793689/94‑1 (MQ=60)
                         CCGTGCCGGTCCCCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGAC                                                                      <  SRR4302540.7528614/94‑1 (MQ=60)
                          atctagcgcttCCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACC                                                                     <  SRR4302540.565151/83‑1 (MQ=60)
                          CGTGCCGGGCCCCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACC                                                                     <  SRR4302540.5651670/94‑1 (MQ=60)
                          CGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACC                                                                     <  SRR4302540.1795452/94‑1 (MQ=60)
                           GTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGTAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCG                                                                    <  SRR4302540.7225260/94‑1 (MQ=60)
                           GTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCG                                                                    <  SRR4302540.6638463/94‑1 (MQ=60)
                           GTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCG                                                                    <  SRR4302540.3942731/94‑1 (MQ=60)
                            TGCCGGCCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGC                                                                   <  SRR4302540.6267001/94‑1 (MQ=60)
                             GCCGGGCCCCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCG                                                                  <  SRR4302540.8975696/94‑1 (MQ=60)
                             GCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGTGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCG                                                                  <  SRR4302540.539715/94‑1 (MQ=60)
                             GCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCG                                                                  <  SRR4302540.539714/94‑1 (MQ=60)
                             GCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCG                                                                  <  SRR4302540.9272161/94‑1 (MQ=60)
                              CCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGG                                                                 <  SRR4302540.3776011/94‑1 (MQ=60)
                               CGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATCCCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT                                                                <  SRR4302540.6277716/94‑1 (MQ=60)
                               CGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT                                                                <  SRR4302540.8937218/94‑1 (MQ=60)
                               CGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT                                                                >  SRR4302540.93301/1‑94 (MQ=60)
                                  GCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGCCCGCGGTGTG                                                             >  SRR4302540.8437216/1‑94 (MQ=60)
                                  GCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTC                                                             >  SRR4302540.8437215/1‑94 (MQ=60)
                                   CACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCG                                                            >  SRR4302540.5236422/1‑94 (MQ=60)
                                    ACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGT                                                           <  SRR4302540.9270021/94‑1 (MQ=60)
                                     CCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTT                                                          <  SRR4302540.4464793/94‑1 (MQ=60)
                                       ATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTT                                                        <  SRR4302540.5741154/94‑1 (MQ=60)
                                       ATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTT                                                        >  SRR4302540.9356781/1‑94 (MQ=60)
                                         CCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCA                                                      >  SRR4302540.7955066/1‑94 (MQ=60)
                                         CCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCA                                                      <  SRR4302540.2377919/94‑1 (MQ=60)
                                         CCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCA                                                      <  SRR4302540.3327869/94‑1 (MQ=60)
                                         CCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCA                                                      <  SRR4302540.3327581/94‑1 (MQ=60)
                                          gctcttccgatctagcgcttATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAA                                                     <  SRR4302540.8832557/74‑1 (MQ=60)
                                          CACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAA                                                     <  SRR4302540.8468621/94‑1 (MQ=60)
                                          CACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAA                                                     >  SRR4302540.5953176/1‑94 (MQ=60)
                                           CCGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAG                                                    <  SRR4302540.8569421/94‑1 (MQ=60)
                                           CCGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAG                                                    <  SRR4302540.3410582/94‑1 (MQ=60)
                                           ACGGCAAAATGCCAACTCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAG                                                    <  SRR4302540.8569426/94‑1 (MQ=60)
                                            CGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGT                                                   <  SRR4302540.7027104/94‑1 (MQ=60)
                                            CGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTGGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGT                                                   <  SRR4302540.3699497/94‑1 (MQ=60)
                                              GCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTAC                                                 <  SRR4302540.8256638/94‑1 (MQ=60)
                                              GCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTAC                                                 <  SRR4302540.136411/94‑1 (MQ=60)
                                               CAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACA                                                <  SRR4302540.6734228/94‑1 (MQ=60)
                                                AAAATGCCAACGCGATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACAT                                               <  SRR4302540.5153743/94‑1 (MQ=60)
                                                AAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACAT                                               <  SRR4302540.2471058/94‑1 (MQ=60)
                                                 AAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATA                                              >  SRR4302540.5832737/1‑94 (MQ=60)
                                                  AATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAG                                             <  SRR4302540.5285008/94‑1 (MQ=60)
                                                   ATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGG                                            <  SRR4302540.496378/94‑1 (MQ=60)
                                                   ATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGG                                            <  SRR4302540.8444075/94‑1 (MQ=60)
                                                   ATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGG                                            >  SRR4302540.4214586/1‑94 (MQ=60)
                                                   ATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGG                                            <  SRR4302540.4075191/94‑1 (MQ=60)
                                                    TGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGT                                           <  SRR4302540.4263554/94‑1 (MQ=60)
                                                    TGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGT                                           >  SRR4302540.5427679/1‑94 (MQ=60)
                                                      GCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGA                                         <  SRR4302540.818004/94‑1 (MQ=60)
                                                      CCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGA                                         <  SRR4302540.4763822/94‑1 (MQ=60)
                                                      CCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTATCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGAGA                                         >  SRR4302540.3314241/1‑94 (MQ=60)
                                                       CAACGCCATCGGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAG                                        <  SRR4302540.8378732/94‑1 (MQ=60)
                                                       CAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAG                                        >  SRR4302540.7256634/1‑94 (MQ=60)
                                                       CAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAG                                        <  SRR4302540.9148813/94‑1 (MQ=60)
                                                       CAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAG                                        >  SRR4302540.6110917/1‑94 (MQ=60)
                                                        AACTCGATCCGGGAATACCTCCAGACGCTCGGTGCTGTCTTCCCGCAGTTGGCGAGTGAAGTCCGCGGTGTCGTTTTCAAGTACATAGGTGAGG                                       <  SRR4302540.1049767/94‑1 (MQ=60)
                                                        AACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGG                                       <  SRR4302540.3057723/94‑1 (MQ=60)
                                                        AACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGCCGTTTTCAAGTACATAGGTGAGG                                       >  SRR4302540.1360682/1‑94 (MQ=60)
                                                        AACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTACCACAGTTGGCGAGTGAAGACCGCGGTGCCGTTTTCAAGTACATAGGTGAGG                                       >  SRR4302540.1360681/1‑94 (MQ=60)
                                                         ACGCCATCCGGGAATACCCCCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGG                                      <  SRR4302540.3006393/94‑1 (MQ=60)
                                                          CGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGC                                     <  SRR4302540.3639074/94‑1 (MQ=60)
                                                          CGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGC                                     <  SRR4302540.2412208/94‑1 (MQ=60)
                                                          CGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGC                                     <  SRR4302540.1309322/94‑1 (MQ=60)
                                                           GCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCG                                    <  SRR4302540.9996182/94‑1 (MQ=60)
                                                            CCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACCTAGGTGAGGGCGA                                   >  SRR4302540.3743495/1‑94 (MQ=60)
                                                             CATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGAT                                  >  SRR4302540.8807600/1‑94 (MQ=60)
                                                             CATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGAT                                  >  SRR4302540.4934759/1‑94 (MQ=60)
                                                              ATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATC                                 >  SRR4302540.9861003/1‑94 (MQ=60)
                                                              ATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATC                                 >  SRR4302540.6017505/1‑94 (MQ=60)
                                                              ATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATC                                 >  SRR4302540.7954254/1‑94 (MQ=60)
                                                              ATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATC                                 <  SRR4302540.4430072/94‑1 (MQ=60)
                                                              ATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATC                                 >  SRR4302540.7134023/1‑94 (MQ=60)
                                                              AGCCGGGAATACCACCAGACACTCGGTGCTGTCTACCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATC                                 <  SRR4302540.2564884/94‑1 (MQ=60)
                                                               TCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCA                                <  SRR4302540.9005042/94‑1 (MQ=60)
                                                               TCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCA                                <  SRR4302540.9005043/94‑1 (MQ=60)
                                                                CCGGGAATACCGCCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAG                               <  SRR4302540.3481699/94‑1 (MQ=60)
                                                                CCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAG                               <  SRR4302540.3837806/94‑1 (MQ=60)
                                                                CCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAG                               <  SRR4302540.1490653/94‑1 (MQ=60)
                                                                 CGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGG                              <  SRR4302540.8673321/94‑1 (MQ=60)
                                                                 CGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGG                              <  SRR4302540.9444077/94‑1 (MQ=60)
                                                                 CGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGG                              <  SRR4302540.8969012/94‑1 (MQ=60)
                                                                 CGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGG                              >  SRR4302540.4630674/1‑94 (MQ=60)
                                                                 CGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGG                              <  SRR4302540.8969011/94‑1 (MQ=60)
                                                                 CGGGAATACCACCAGACACTCGGTGCTGGCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGG                              <  SRR4302540.3501031/94‑1 (MQ=60)
                                                                  GGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGT                             >  SRR4302540.6665444/1‑94 (MQ=60)
                                                                  GGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGCGTCGTTTTCAAGGACATAGGTGAGGGCGATCAGGT                             >  SRR4302540.5352332/1‑94 (MQ=60)
                                                                   GGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTT                            <  SRR4302540.4754174/94‑1 (MQ=60)
                                                                    GAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTG                           >  SRR4302540.5546613/1‑94 (MQ=60)
                                                                     AATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGG                          >  SRR4302540.204826/1‑94 (MQ=60)
                                                                      ATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGT                         >  SRR4302540.2727829/1‑94 (MQ=60)
                                                                      ATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGT                         >  SRR4302540.27058/1‑94 (MQ=60)
                                                                      ATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGT                         >  SRR4302540.5861299/1‑94 (MQ=60)
                                                                      ATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGT                         <  SRR4302540.4419575/94‑1 (MQ=60)
                                                                      ATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGT                         <  SRR4302540.4989661/94‑1 (MQ=60)
                                                                        ACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGG                       <  SRR4302540.2703083/94‑1 (MQ=60)
                                                                        ACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGG                       <  SRR4302540.8656198/94‑1 (MQ=60)
                                                                         CCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGC                      >  SRR4302540.8001870/1‑94 (MQ=60)
                                                                          CACCAGACACTCGGTGCTGTCTTCCCACAGTTTGCGAGTGAAGACCGCGGTGTCGTTGTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCG                     >  SRR4302540.8625191/1‑94 (MQ=60)
                                                                          CACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGCTCAGGTTGGGGGCG                     >  SRR4302540.1605862/1‑94 (MQ=60)
                                                                          CACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGGGTCGTTTTCAAGTACATAGGTGAGGTCGATCAGGTTGGTGGCG                     >  SRR4302540.8625190/1‑94 (MQ=60)
                                                                           ACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGT                    <  SRR4302540.496652/94‑1 (MQ=60)
                                                                           ACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGT                    <  SRR4302540.6380993/94‑1 (MQ=60)
                                                                            CCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTG                   <  SRR4302540.7355377/94‑1 (MQ=60)
                                                                            CCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTG                   <  SRR4302540.697412/94‑1 (MQ=60)
                                                                            CCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTG                   <  SRR4302540.8034281/94‑1 (MQ=60)
                                                                            CCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTG                   <  SRR4302540.2655683/94‑1 (MQ=60)
                                                                            CCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGGTGGTGGCGTG                   >  SRR4302540.3903395/1‑94 (MQ=60)
                                                                             CAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGG                  <  SRR4302540.6999823/94‑1 (MQ=60)
                                                                               GACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCA                >  SRR4302540.8081354/1‑94 (MQ=60)
                                                                               GACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCA                <  SRR4302540.8026450/94‑1 (MQ=60)
                                                                                GCACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAG               <  SRR4302540.6241451/94‑1 (MQ=60)
                                                                                 CACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGT              <  SRR4302540.9322424/94‑1 (MQ=60)
                                                                                 CACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGT              <  SRR4302540.6944975/94‑1 (MQ=60)
                                                                                  ACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTG             >  SRR4302540.2266014/1‑94 (MQ=60)
                                                                                  ACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTG             >  SRR4302540.7088515/1‑94 (MQ=60)
                                                                                  ACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTG             >  SRR4302540.4778235/1‑94 (MQ=60)
                                                                                   CTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGC            <  SRR4302540.5205023/94‑1 (MQ=60)
                                                                                   CTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGC            <  SRR4302540.1991580/94‑1 (MQ=60)
                                                                                    TCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGAGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCA           >  SRR4302540.208908/1‑94 (MQ=60)
                                                                                     CGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCAT          >  SRR4302540.1125084/1‑94 (MQ=60)
                                                                                     CGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCAT          <  SRR4302540.5111052/94‑1 (MQ=60)
                                                                                     CGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCAT          >  SRR4302540.5105372/1‑94 (MQ=60)
                                                                                     CGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCAT          >  SRR4302540.7562485/1‑94 (MQ=60)
                                                                                     CGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCAT          <  SRR4302540.5329683/94‑1 (MQ=60)
                                                                                     CGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCAT          <  SRR4302540.8177821/94‑1 (MQ=60)
                                                                                     CGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCAG          >  SRR4302540.1125083/1‑94 (MQ=60)
                                                                                      GGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATG         >  SRR4302540.2833629/1‑94 (MQ=60)
                                                                                      GGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATG         >  SRR4302540.1127056/1‑94 (MQ=60)
                                                                                       GTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGA        >  SRR4302540.2996838/1‑94 (MQ=60)
                                                                                       GTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGA        <  SRR4302540.1666619/94‑1 (MQ=60)
                                                                                       GTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGA        <  SRR4302540.8558144/94‑1 (MQ=60)
                                                                                       GTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGA        <  SRR4302540.8420533/94‑1 (MQ=60)
                                                                                        TGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGCGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGAT       >  SRR4302540.8175598/1‑94 (MQ=60)
                                                                                        TGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCTGTGCATGAT       <  SRR4302540.8979589/94‑1 (MQ=60)
                                                                                        TGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGAT       <  SRR4302540.10038101/94‑1 (MQ=60)
                                                                                        TGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGAT       >  SRR4302540.8754210/1‑94 (MQ=60)
                                                                                         GCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATC      >  SRR4302540.5002166/1‑94 (MQ=60)
                                                                                         GCTGGCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATC      <  SRR4302540.3950181/94‑1 (MQ=60)
                                                                                          CTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCA     >  SRR4302540.4068961/1‑94 (MQ=60)
                                                                                          CTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCA     >  SRR4302540.8849357/1‑94 (MQ=60)
                                                                                           TGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCAC    <  SRR4302540.1762403/94‑1 (MQ=60)
                                                                                           TGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCAC    >  SRR4302540.7948748/1‑94 (MQ=60)
                                                                                           TGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCAC    >  SRR4302540.6259258/1‑94 (MQ=60)
                                                                                           TGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGGGGCGGGGCAGTGCATGATCAC    >  SRR4302540.7053470/1‑94 (MQ=60)
                                                                                            GTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACC   <  SRR4302540.8764632/94‑1 (MQ=60)
                                                                                            GTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACC   >  SRR4302540.6686759/1‑94 (MQ=60)
                                                                                            GTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACC   <  SRR4302540.3481638/94‑1 (MQ=60)
                                                                                            GTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGACCAGGTTGGTGGCGTGGCAGTGCATGATCACC   <  SRR4302540.3085420/94‑1 (MQ=60)
                                                                                             TCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACCC  <  SRR4302540.3706208/94‑1 (MQ=60)
                                                                                             |                                                                                             
TGTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACCC  >  NC_000913/4091258‑4091444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑