Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,091,351 C→T G168D (GGC→GAC)  rhaD ← rhamnulose‑phosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,091,3510CT100.0% 882.6 / NA 237G168D (GGC→GAC) rhaDrhamnulose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (115/122);  total (115/122)

Only 100 of 252 total aligned reads displayed.

GCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATC  >  NC_000913/4091261‑4091440
                                                                                          |                                                                                          
gCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCt                                                                                          >  1:2937468/1‑93 (MQ=255)
 cGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCtt                                                                                         <  2:5473846/93‑1 (MQ=255)
  ggTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTc                                                                                        <  2:5597183/93‑1 (MQ=255)
   gTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTcc                                                                                       >  1:3269120/1‑93 (MQ=255)
    tCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTccc                                                                                      <  2:83176/93‑1 (MQ=255)
    tCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTccc                                                                                      >  1:4133144/1‑93 (MQ=255)
    tCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTccc                                                                                      <  2:23627/93‑1 (MQ=255)
       ccTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCcaca                                                                                   <  1:2492216/93‑1 (MQ=255)
        cTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAg                                                                                  >  1:2602532/1‑93 (MQ=255)
        cTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAg                                                                                  <  1:1133981/93‑1 (MQ=255)
         tGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGt                                                                                 >  2:24142/1‑93 (MQ=255)
         tGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGt                                                                                 <  2:4787778/93‑1 (MQ=255)
         tGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGt                                                                                 >  1:6066179/1‑93 (MQ=255)
         tGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGt                                                                                 >  1:422940/1‑93 (MQ=255)
          tgCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGtt                                                                                <  2:2970369/92‑1 (MQ=255)
          ggCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGtt                                                                                >  1:878222/1‑93 (MQ=255)
          ggCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCAATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGtt                                                                                >  1:3080096/1‑93 (MQ=255)
           gCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACGGTTg                                                                               >  2:5817492/1‑93 (MQ=255)
             cGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGc                                                                             >  2:2695860/1‑93 (MQ=255)
             cGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGc                                                                             >  1:4682529/1‑93 (MQ=255)
              gATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCg                                                                            >  2:1363515/1‑93 (MQ=255)
               aTTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGa                                                                           >  1:4978557/1‑93 (MQ=255)
               aTTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGa                                                                           >  2:1129382/1‑93 (MQ=255)
               aTTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGa                                                                           >  1:1379515/1‑93 (MQ=255)
               aTTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGa                                                                           >  1:211564/1‑93 (MQ=255)
                tttCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAg                                                                          >  2:4919235/1‑93 (MQ=255)
                 ttCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGt                                                                         <  2:2867942/93‑1 (MQ=255)
                   cGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGa                                                                       <  2:1619055/93‑1 (MQ=255)
                   cGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGa                                                                       <  1:1714269/93‑1 (MQ=255)
                   cGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGa                                                                       <  2:5311791/93‑1 (MQ=255)
                   cGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGa                                                                       >  1:3513513/1‑93 (MQ=255)
                     tCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAg                                                                     >  2:3450783/1‑93 (MQ=255)
                     tCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAg                                                                     <  2:3564584/93‑1 (MQ=255)
                     tCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAg                                                                     >  2:3627938/1‑93 (MQ=255)
                      ccGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGa                                                                    <  1:3560190/93‑1 (MQ=255)
                        gTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATCCCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGAcc                                                                  <  1:4041845/93‑1 (MQ=255)
                        gTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGAcc                                                                  <  2:3460041/93‑1 (MQ=255)
                         tGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcg                                                                 <  2:2059149/93‑1 (MQ=255)
                          gCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcgc                                                                >  2:5206848/1‑93 (MQ=255)
                           ccGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACcgcg                                                               <  2:2257461/93‑1 (MQ=255)
                             gggCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgt                                                             <  1:2347520/93‑1 (MQ=255)
                               gccccATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgt‑gt                                                          <  1:3311488/90‑1 (MQ=255)
                               gCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgt‑gt                                                          >  2:2277681/1‑93 (MQ=255)
                               gCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgt‑gt                                                          <  1:1000569/93‑1 (MQ=255)
                               gCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGgt‑gt                                                          <  1:387636/93‑1 (MQ=255)
                                 aCCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCg                                                        >  2:1237955/1‑93 (MQ=255)
                                   cATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGtt                                                      >  2:3826267/1‑93 (MQ=255)
                                    aTCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGttt                                                     <  1:4641087/93‑1 (MQ=255)
                                        aCGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCaa                                                 <  2:4494048/93‑1 (MQ=255)
                                         cGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAg                                                >  1:1963178/1‑93 (MQ=255)
                                         cGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAg                                                <  2:2515816/93‑1 (MQ=255)
                                          ggCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGt                                               >  2:3336118/1‑93 (MQ=255)
                                           gCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTa                                              >  2:6248865/1‑93 (MQ=255)
                                             aaaaTGGCAGCGCCATCCGTGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACa                                            <  2:3275825/93‑1 (MQ=255)
                                             aaaaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACa                                            <  1:1021192/93‑1 (MQ=255)
                                              agaTGCCAACGCCATCCGGGAATACCACCAGTCTCTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACAt                                           <  1:2683643/91‑1 (MQ=255)
                                              aaaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACAt                                           >  1:5892278/1‑93 (MQ=255)
                                               aaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATa                                          <  2:4247818/93‑1 (MQ=255)
                                               aaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATa                                          <  1:3198280/93‑1 (MQ=255)
                                               aaTGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATa                                          <  1:3624522/93‑1 (MQ=255)
                                                  gCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGt                                       <  2:2155015/93‑1 (MQ=255)
                                                  gCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGt                                       <  1:4807750/93‑1 (MQ=255)
                                                  gCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGt                                       <  1:2416562/93‑1 (MQ=255)
                                                     aaCGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAg                                    <  1:2267474/93‑1 (MQ=255)
                                                     aaCGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAg                                    >  1:738897/1‑93 (MQ=255)
                                                      aCGCCATCCGGGAATACCACCAGGCACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAgg                                   <  1:4842018/93‑1 (MQ=255)
                                                      aCGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAgg                                   >  2:46407/1‑93 (MQ=255)
                                                         ccATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCg                                <  1:1788180/93‑1 (MQ=255)
                                                          cATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGa                               >  2:3186071/1‑93 (MQ=255)
                                                           aTCCGGGAAGACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGAt                              <  2:5049501/93‑1 (MQ=255)
                                                            tCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATc                             >  2:359057/1‑93 (MQ=255)
                                                            accGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGAtg                             >  2:6050942/2‑92 (MQ=255)
                                                              cGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAg                           >  2:2675148/1‑93 (MQ=255)
                                                              cGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAg                           >  1:3696414/1‑93 (MQ=255)
                                                               gggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAgg                          >  2:5670428/1‑93 (MQ=255)
                                                                ggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGt                         <  1:1929216/93‑1 (MQ=255)
                                                                ggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGt                         >  2:4114409/1‑93 (MQ=255)
                                                                ggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGt                         >  1:2058859/1‑93 (MQ=255)
                                                                ggAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGt                         <  2:2598506/93‑1 (MQ=255)
                                                                ggAATACCACCAGACACTCGGTGCTGCCTTACCACAGTTGGCGAGTGAAGACCGCGTT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGt                         <  2:5776812/93‑1 (MQ=255)
                                                                  aaTACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtg                       <  2:1848407/93‑1 (MQ=255)
                                                                  aaTACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtg                       <  1:5168067/93‑1 (MQ=255)
                                                                   aTACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtgg                      >  2:2306012/1‑93 (MQ=255)
                                                                    tACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtggt                     <  2:3723217/93‑1 (MQ=255)
                                                                      ccaccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTtggtgg                   <  1:4372540/93‑1 (MQ=255)
                                                                       caccaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGGAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggc                  >  2:2755328/1‑93 (MQ=255)
                                                                        accaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGTAGATCTCGGTGGTCG‑TCTCAAGAATATAGGTGAGGGCGATCAGGTTGgtggcg                 >  2:642917/1‑93 (MQ=255)
                                                                        accaGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcg                 >  1:1520226/1‑93 (MQ=255)
                                                                          caGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgtg               >  1:143817/1‑93 (MQ=255)
                                                                           aGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgtgg              <  1:6233475/93‑1 (MQ=255)
                                                                            gACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GGCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGgtggcgtggc             >  1:546345/1‑93 (MQ=255)
                                                                              cacTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAg           <  2:1509707/93‑1 (MQ=255)
                                                                               acTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGt          <  1:530611/93‑1 (MQ=255)
                                                                                 tCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGc        <  1:5625880/93‑1 (MQ=255)
                                                                                 tCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGc        >  2:4456348/1‑93 (MQ=255)
                                                                                 tCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGc        <  2:458817/93‑1 (MQ=255)
                                                                                  cGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCa       >  2:5952428/1‑93 (MQ=255)
                                                                                    gTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATg     >  1:2812319/1‑93 (MQ=255)
                                                                                      gctgtCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGAt   >  2:1055936/1‑93 (MQ=255)
                                                                                       ctgtCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATc  >  2:3099464/1‑93 (MQ=255)
                                                                                          |                                                                                          
GCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGT‑GTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATC  >  NC_000913/4091261‑4091440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACC  >  NC_000913/4091259‑4091443
                                                                                            |                                                                                            
GTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTC                                                                                             >  SRR4302533.3094615/1‑94 (MQ=60)
GTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTC                                                                                             >  SRR4302533.4684838/1‑94 (MQ=60)
GTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTC                                                                                             >  SRR4302533.171382/1‑94 (MQ=60)
 TGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCT                                                                                            >  SRR4302533.2955421/1‑94 (MQ=60)
 TGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCT                                                                                            <  SRR4302533.4691965/94‑1 (MQ=60)
    GGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCC                                                                                         <  SRR4302533.3003835/94‑1 (MQ=60)
    GGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCC                                                                                         >  SRR4302533.3289287/1‑94 (MQ=60)
     GTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCC                                                                                        >  SRR4302533.4158234/1‑94 (MQ=60)
       CGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCAC                                                                                      >  SRR4302533.3250273/1‑94 (MQ=60)
       CGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCAC                                                                                      >  SRR4302533.4704489/1‑94 (MQ=60)
        GCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACA                                                                                     >  SRR4302533.4214241/1‑94 (MQ=60)
         CCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAG                                                                                    <  SRR4302533.2065892/94‑1 (MQ=60)
         CCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAG                                                                                    >  SRR4302533.2618524/1‑94 (MQ=60)
         CCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAG                                                                                    <  SRR4302533.2507564/94‑1 (MQ=60)
         CCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAG                                                                                    <  SRR4302533.5531784/94‑1 (MQ=60)
          CTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGT                                                                                   >  SRR4302533.516554/1‑94 (MQ=60)
          CTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGT                                                                                   >  SRR4302533.6102891/1‑94 (MQ=60)
          CTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGT                                                                                   <  SRR4302533.1140722/94‑1 (MQ=60)
          CTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGT                                                                                   >  SRR4302533.4525121/1‑94 (MQ=60)
          CTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGT                                                                                   >  SRR4302533.425471/1‑94 (MQ=60)
          CTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCAAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCCCAGT                                                                                   >  SRR4302533.3663650/1‑94 (MQ=60)
           TGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTT                                                                                  >  SRR4302533.883378/1‑94 (MQ=60)
           TGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTT                                                                                  >  SRR4302533.319496/1‑94 (MQ=60)
           TGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTT                                                                                  <  SRR4302533.2151011/94‑1 (MQ=60)
           TGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCAATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTT                                                                                  >  SRR4302533.3098994/1‑94 (MQ=60)
             GCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGG                                                                                >  SRR4302533.5680792/1‑94 (MQ=60)
              CCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGC                                                                               >  SRR4302533.4710899/1‑94 (MQ=60)
               CGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCG                                                                              >  SRR4302533.1528752/1‑94 (MQ=60)
               CGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCG                                                                              <  SRR4302533.459367/94‑1 (MQ=60)
                GATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATCCCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGA                                                                             >  SRR4302533.3292398/1‑94 (MQ=60)
                GATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGA                                                                             >  SRR4302533.5008746/1‑94 (MQ=60)
                GATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGA                                                                             >  SRR4302533.212898/1‑94 (MQ=60)
                GATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGA                                                                             >  SRR4302533.690715/1‑94 (MQ=60)
                GATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGA                                                                             >  SRR4302533.1387816/1‑94 (MQ=60)
                 ATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAG                                                                            <  SRR4302533.6093224/94‑1 (MQ=60)
                 ATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATAACACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAG                                                                            >  SRR4302533.1089901/1‑94 (MQ=60)
                  TTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGT                                                                           <  SRR4302533.5289363/94‑1 (MQ=60)
                   TCCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTG                                                                          <  SRR4302533.3809494/94‑1 (MQ=60)
                    TCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGA                                                                         >  SRR4302533.3535173/1‑94 (MQ=60)
                     CGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAA                                                                        <  SRR4302533.1724661/94‑1 (MQ=60)
                      GTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAG                                                                       >  SRR4302533.3961614/1‑94 (MQ=60)
                      GTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAG                                                                       >  SRR4302533.2775835/1‑94 (MQ=60)
                       TCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGA                                                                      >  SRR4302533.3066857/1‑94 (MQ=60)
                        CCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGAC                                                                     <  SRR4302533.3582124/94‑1 (MQ=60)
                         CGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACC                                                                    <  SRR4302533.5551465/94‑1 (MQ=60)
                         CGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACC                                                                    <  SRR4302533.2666217/94‑1 (MQ=60)
                          GTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATCCCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCG                                                                   <  SRR4302533.4066444/94‑1 (MQ=60)
                           TGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGC                                                                  >  SRR4302533.2364693/1‑94 (MQ=60)
                               GGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTG                                                              <  SRR4302533.2362044/94‑1 (MQ=60)
                               GGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTG                                                              >  SRR4302533.2746871/1‑94 (MQ=60)
                                GGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGGTGTCTTTCCACAGTTGGGGAGTGAAGACCGCGGTGG                                                             >  SRR4302533.2283026/1‑94 (MQ=60)
                                 GCCCCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTC                                                            <  SRR4302533.3331938/94‑1 (MQ=60)
                                 GCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTC                                                            <  SRR4302533.389972/94‑1 (MQ=60)
                                 GCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTC                                                            <  SRR4302533.1006409/94‑1 (MQ=60)
                                 GCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCCGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTA                                                            <  SRR4302533.856280/94‑1 (MQ=60)
                                  CACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCG                                                           >  SRR4302533.1622256/1‑94 (MQ=60)
                                  CACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCG                                                           >  SRR4302533.4099832/1‑94 (MQ=60)
                                   ACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGT                                                          >  SRR4302533.4194522/1‑94 (MQ=60)
                                     CATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTT                                                        >  SRR4302533.1017443/1‑94 (MQ=60)
                                     CATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTT                                                        >  SRR4302533.2550904/1‑94 (MQ=60)
                                      ATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTT                                                       <  SRR4302533.4669207/94‑1 (MQ=60)
                                      ATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTT                                                       >  SRR4302533.2419045/1‑94 (MQ=60)
                                       TCCACGGCAAAATGCCAAGGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTC                                                      <  SRR4302533.1943943/94‑1 (MQ=60)
                                         CACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAA                                                    >  SRR4302533.1434820/1‑94 (MQ=60)
                                          ACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAG                                                   >  SRR4302533.1975163/1‑94 (MQ=60)
                                            GGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTA                                                 >  SRR4302533.4799053/1‑94 (MQ=60)
                                            GGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTA                                                 <  SRR4302533.4730508/94‑1 (MQ=60)
                                             GCAAAATGCCAACGCCATCCGGGAATACCACCAGACGCTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTAC                                                <  SRR4302533.2875581/94‑1 (MQ=60)
                                              CAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACA                                               <  SRR4302533.3260811/94‑1 (MQ=60)
                                               AAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACAT                                              <  SRR4302533.1027193/94‑1 (MQ=60)
                                               AAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACAT                                              >  SRR4302533.5927868/1‑94 (MQ=60)
                                                AGATGCCAACGCCATCCGGGAATACCACCAGTCTCTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATA                                             <  SRR4302533.2700087/94‑1 (MQ=60)
                                                 AATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAG                                            <  SRR4302533.5953895/94‑1 (MQ=60)
                                                 AATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAG                                            <  SRR4302533.3646825/94‑1 (MQ=60)
                                                 AATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAG                                            <  SRR4302533.3217963/94‑1 (MQ=60)
                                                    GCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTG                                         <  SRR4302533.2431488/94‑1 (MQ=60)
                                                    GCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTG                                         <  SRR4302533.4836858/94‑1 (MQ=60)
                                                    GCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTG                                         <  SRR4302533.1992646/94‑1 (MQ=60)
                                                    GCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACAGCGGTGTCGTTTTCAAGTACATAGGTG                                         <  SRR4302533.2233665/94‑1 (MQ=60)
                                                     CCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGA                                        >  SRR4302533.2557387/1‑94 (MQ=60)
                                                      CAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAG                                       >  SRR4302533.743250/1‑94 (MQ=60)
                                                      CAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAG                                       <  SRR4302533.712160/94‑1 (MQ=60)
                                                      CAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAG                                       <  SRR4302533.3492121/94‑1 (MQ=60)
                                                       AACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGG                                      <  SRR4302533.1416396/94‑1 (MQ=60)
                                                       AACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGG                                      <  SRR4302533.2281499/94‑1 (MQ=60)
                                                        ACGCCATCCGGGAATACCACCAGGCACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGG                                     <  SRR4302533.4871363/94‑1 (MQ=60)
                                                        ACGCCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGG                                     <  SRR4302533.114806/94‑1 (MQ=60)
                                                           CCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGA                                  <  SRR4302533.1799049/94‑1 (MQ=60)
                                                           CCATCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGA                                  >  SRR4302533.6252252/1‑94 (MQ=60)
                                                              TCCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCA                               <  SRR4302533.2461458/94‑1 (MQ=60)
                                                               CCGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAG                              >  SRR4302533.3719119/1‑94 (MQ=60)
                                                                CGGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGG                             <  SRR4302533.357389/94‑1 (MQ=60)
                                                                 GGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGT                            >  SRR4302533.2071553/1‑94 (MQ=60)
                                                                 GGGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGT                            >  SRR4302533.3673766/1‑94 (MQ=60)
                                                                  GGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTT                           <  SRR4302533.1790675/94‑1 (MQ=60)
                                                                  GGAATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTT                           <  SRR4302533.1940987/94‑1 (MQ=60)
                                                                    AATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGG                         <  SRR4302533.4276372/94‑1 (MQ=60)
                                                                    AATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGG                         <  SRR4302533.3580552/94‑1 (MQ=60)
                                                                    AATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGG                         <  SRR4302533.5199264/94‑1 (MQ=60)
                                                                     ATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGT                        <  SRR4302533.4881148/94‑1 (MQ=60)
                                                                     ATACCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGG                        >  SRR4302533.4942389/1‑94 (MQ=60)
                                                                        CCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGC                     >  SRR4302533.1840307/1‑94 (MQ=60)
                                                                        CCACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGC                     <  SRR4302533.4398931/94‑1 (MQ=60)
                                                                         CACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCG                    >  SRR4302533.1529378/1‑94 (MQ=60)
                                                                          ACCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGG                   >  SRR4302533.5280863/1‑94 (MQ=60)
                                                                           CCAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTG                  >  SRR4302533.144740/1‑94 (MQ=60)
                                                                            CAGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGG                 >  SRR4302533.3429808/1‑94 (MQ=60)
                                                                             AGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGC                <  SRR4302533.6270798/94‑1 (MQ=60)
                                                                             AGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGC                >  SRR4302533.1683785/1‑94 (MQ=60)
                                                                             AGACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGGCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGC                >  SRR4302533.549591/1‑94 (MQ=60)
                                                                               ACACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAG              <  SRR4302533.1921201/94‑1 (MQ=60)
                                                                                 ACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTG            <  SRR4302533.533767/94‑1 (MQ=60)
                                                                                 ACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTG            <  SRR4302533.5479135/94‑1 (MQ=60)
                                                                                 ACTCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTG            <  SRR4302533.4838396/94‑1 (MQ=60)
                                                                                   TCGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCA          <  SRR4302533.5659895/94‑1 (MQ=60)
                                                                                    CGGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCAG         >  SRR4302533.3935136/1‑94 (MQ=60)
                                                                                     GGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATG        >  SRR4302533.2829628/1‑94 (MQ=60)
                                                                                     GGTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATG        <  SRR4302533.1047239/94‑1 (MQ=60)
                                                                                      GTGCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGA       <  SRR4302533.3640020/94‑1 (MQ=60)
                                                                                        GCTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATC     <  SRR4302533.2126526/94‑1 (MQ=60)
                                                                                         CTGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCA    >  SRR4302533.5751522/1‑94 (MQ=60)
                                                                                          TGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCAC   >  SRR4302533.2419161/1‑94 (MQ=60)
                                                                                          TGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCAC   >  SRR4302533.2340676/1‑94 (MQ=60)
                                                                                          TGTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCAC   >  SRR4302533.5301487/1‑94 (MQ=60)
                                                                                           GTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACC  >  SRR4302533.274434/1‑94 (MQ=60)
                                                                                           GTCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGGTGGTGGCGTGGCAGTGGATGATCACC  >  SRR4302533.1136411/1‑94 (MQ=60)
                                                                                            |                                                                                            
GTGCGGTCGCCTGGCCGATTTCGTCCGTGCCGGGCACCATCCACGGCAAAATGCCAACGCCATCCGGGAATACCACCAGACACTCGGTGCTGCCTTCCCACAGTTGGCGAGTGAAGACCGCGGTGTCGTTTTCAAGTACATAGGTGAGGGCGATCAGGTTGGTGGCGTGGCAGTGCATGATCACC  >  NC_000913/4091259‑4091443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑