Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,116,372 T→C intergenic (+32/+53) yiiU → / ← menG hypothetical protein/MenG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,116,3720TC99.8% 1634.7 / ‑5.1 497intergenic (+32/+53)yiiU/menGhypothetical protein/MenG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (227/269);  minor base A (1/0);  total (228/269)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.47e-01

Only 100 of 516 total aligned reads displayed.

AGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATC  >  NC_000913/4116282‑4116461
                                                                                          |                                                                                         
 gcagAACGGCTGACAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACcgtg                                                                                        >  2:5615710/1‑93 (MQ=255)
  cagAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCgtgt                                                                                       >  1:9528670/1‑93 (MQ=255)
  cagAACGGCTGGCAGGAACGTCTGCAGGCCCTGCT‑GGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTa                                                                                      <  1:5095617/93‑1 (MQ=255)
   agAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTa                                                                                      >  1:4421918/1‑93 (MQ=255)
   agAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTa                                                                                      <  2:4844625/93‑1 (MQ=255)
   agAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTa                                                                                      <  1:4603653/93‑1 (MQ=255)
   agAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTa                                                                                      >  1:2822029/1‑93 (MQ=255)
   agAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTa                                                                                      >  2:2993442/1‑93 (MQ=255)
    gAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAg                                                                                     >  2:3342695/1‑93 (MQ=255)
    gAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGGCTGATTACACTTCATCTTCCGCGTAATTTCTTTACCGTGTAg                                                                                     >  1:4202057/1‑93 (MQ=255)
    gAACGGCTGGCAGGAACGTCTGCAGGCCCTCCTGGGTCGCGTGGAAGAGGGCTGATTCCGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAg                                                                                     >  2:3809051/1‑93 (MQ=255)
     aaCGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAgg                                                                                    >  2:4032485/1‑93 (MQ=255)
     aaCGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAgg                                                                                    >  2:5821441/1‑93 (MQ=255)
      aCGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATATTCCGCGTAATTTCTTTACCGTGTCGGc                                                                                   >  1:4674356/1‑93 (MQ=255)
         gCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCaa                                                                                >  1:9435190/1‑93 (MQ=255)
         gCTGGCAGGAACGTCTGCAGGCCCTGCTGGGGCGCATGGAAGAGGTCTGATTAAGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCaa                                                                                >  1:6198608/1‑93 (MQ=255)
          cTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAAc                                                                               <  1:2068528/93‑1 (MQ=255)
           tGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGTCAACa                                                                              >  1:3852548/1‑93 (MQ=255)
           tGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACa                                                                              <  2:9915321/93‑1 (MQ=255)
           tGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACa                                                                              <  1:3278842/93‑1 (MQ=255)
            ggCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAg                                                                             >  2:7264950/1‑93 (MQ=255)
             gCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAgg                                                                            >  2:3681655/1‑93 (MQ=255)
             gCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAgg                                                                            >  1:5604740/1‑93 (MQ=255)
                ggAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTac                                                                         >  2:3595055/1‑92 (MQ=255)
                ggAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTaa                                                                         >  1:2552402/1‑93 (MQ=255)
                ggAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTaa                                                                         >  2:2238894/1‑93 (MQ=255)
                 gAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAAc                                                                        <  1:8775653/93‑1 (MQ=255)
                 gAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAAc                                                                        <  2:3530982/93‑1 (MQ=255)
                   aCGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGc                                                                      <  1:7883930/93‑1 (MQ=255)
                      tCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGa                                                                   >  2:1196557/1‑93 (MQ=255)
                      tCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGa                                                                   >  1:8316105/1‑93 (MQ=255)
                       cTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGaa                                                                  >  2:534787/1‑93 (MQ=255)
                        tGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGaaa                                                                 >  1:6477145/1‑93 (MQ=255)
                        tGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGaaa                                                                 >  2:7893369/1‑93 (MQ=255)
                          cAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGaaaaa                                                               <  1:1527709/93‑1 (MQ=255)
                              cccTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGc                                                           <  2:1421909/93‑1 (MQ=255)
                               ccTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCa                                                          <  2:7504772/93‑1 (MQ=255)
                                 tgctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCAcc                                                        >  1:5378160/1‑93 (MQ=255)
                                 tgctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCAcc                                                        <  2:5116928/93‑1 (MQ=255)
                                  gctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCt                                                       >  2:8661935/1‑93 (MQ=255)
                                  gctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCt                                                       >  1:2356037/1‑93 (MQ=255)
                                  gctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCt                                                       <  2:6904846/93‑1 (MQ=255)
                                  gctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCt                                                       <  1:6661462/93‑1 (MQ=255)
                                   gtgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCtt                                                      <  1:7354881/92‑1 (MQ=255)
                                    tgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTg                                                     <  2:4144655/93‑1 (MQ=255)
                                     gggTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTgg                                                    >  2:8043701/1‑92 (MQ=255)
                                      ggTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCg                                                   <  2:1013351/93‑1 (MQ=255)
                                       gTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCgg                                                  <  1:1219002/93‑1 (MQ=255)
                                        tCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGt                                                 <  2:2431649/93‑1 (MQ=255)
                                        tCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGt                                                 >  1:10007898/1‑93 (MQ=255)
                                         cGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTg                                                <  2:9192541/93‑1 (MQ=255)
                                           cATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGcc                                              <  1:6326509/93‑1 (MQ=255)
                                             tGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCtt                                            <  2:8285677/93‑1 (MQ=255)
                                              ggAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTACCGCAGAAAAAAGGCGCCTTGCGGTGCCttt                                           <  2:543658/93‑1 (MQ=255)
                                               gAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTc                                          <  2:3053649/93‑1 (MQ=255)
                                               gAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGCGCCtttt                                          >  1:7727861/1‑92 (MQ=255)
                                                aaGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCt                                         >  1:1660325/1‑93 (MQ=255)
                                                 agagGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCtt                                        >  1:4873883/1‑93 (MQ=255)
                                                    ggTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGGCAACAGGTAACGCAGAAAAAAGGCAACTTGCGGGGCCTTTCTTATc                                     >  2:6002088/1‑93 (MQ=255)
                                                    ggTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGGCCTTCTTAtt                                     >  2:4288922/1‑92 (MQ=255)
                                                    ggTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATc                                     <  1:7481699/93‑1 (MQ=255)
                                                     gTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCa                                    >  1:5813332/1‑93 (MQ=255)
                                                      tCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGTCTTTCTTATCAt                                   >  2:2711573/1‑93 (MQ=255)
                                                      tCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCAt                                   <  2:3205889/93‑1 (MQ=255)
                                                      tCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCAt                                   <  2:7050703/93‑1 (MQ=255)
                                                       cTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCAtt                                  <  1:9685522/93‑1 (MQ=255)
                                                       cTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCAtt                                  <  1:5501430/93‑1 (MQ=255)
                                                          aTTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCaa                               >  2:9514005/1‑93 (MQ=255)
                                                          aTTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCaa                               <  2:9090310/93‑1 (MQ=255)
                                                          aTTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCaa                               >  1:8497663/1‑93 (MQ=255)
                                                             aCGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAatat                            <  1:839511/93‑1 (MQ=255)
                                                               gCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATcc                          <  1:7664917/93‑1 (MQ=255)
                                                               gCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATcc                          >  1:6027722/1‑93 (MQ=255)
                                                               gCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATcc                          >  1:1612083/1‑93 (MQ=255)
                                                                cTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCa                         >  2:6990540/1‑93 (MQ=255)
                                                                cTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCa                         >  1:3545042/1‑93 (MQ=255)
                                                                cTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCa                         >  1:397240/1‑93 (MQ=255)
                                                                  tCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACCGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATAATTCAATATCCAGc                       >  2:7760598/1‑93 (MQ=255)
                                                                    aTCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCgg                     >  2:839088/1‑93 (MQ=255)
                                                                     tCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGa                    <  1:7068665/93‑1 (MQ=255)
                                                                       ttCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtc                  <  1:9181366/93‑1 (MQ=255)
                                                                       ttCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtc                  >  1:6914160/1‑93 (MQ=255)
                                                                       ttCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCAGAtc                  >  2:9761989/1‑93 (MQ=255)
                                                                        tCCGCCTAATGTCTGTGCCGTTGAGGCCAACAGGTAACGCAGAAAAAAGGCCCCTGGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtct                 <  1:2929655/93‑1 (MQ=255)
                                                                         ccGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtctt                <  1:1809181/93‑1 (MQ=255)
                                                                         ccGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtctt                >  2:6637983/1‑93 (MQ=255)
                                                                          cgcgTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtcttc               >  1:9940506/1‑93 (MQ=255)
                                                                              tAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGaa           >  2:849013/1‑93 (MQ=255)
                                                                              tAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGaa           >  1:2658652/1‑93 (MQ=255)
                                                                              tAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATAT‑CAACGGATTTTCTTaaa          >  1:1440408/1‑93 (MQ=255)
                                                                               aaTTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGaaa          <  1:3119784/93‑1 (MQ=255)
                                                                                aTTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGGACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAg         >  2:4497560/1‑93 (MQ=255)
                                                                                 tttCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCCGCGGATCTTCTGAAAGa        >  1:8177285/1‑93 (MQ=255)
                                                                                 tttCTTGACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGa        <  1:6990137/93‑1 (MQ=255)
                                                                                  ttCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaa       <  1:4377474/93‑1 (MQ=255)
                                                                                    cTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaata     <  2:4614702/93‑1 (MQ=255)
                                                                                    cTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaata     <  1:6772764/93‑1 (MQ=255)
                                                                                    cTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaata     <  2:2092188/93‑1 (MQ=255)
                                                                                     tttACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGGGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaataa    >  2:2744516/1‑93 (MQ=255)
                                                                                      ttACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaataat   <  2:5247338/93‑1 (MQ=255)
                                                                                          |                                                                                         
AGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATC  >  NC_000913/4116282‑4116461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 296 total aligned reads displayed.
AACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGG  >  NC_000913/4116279‑4116465
                                                                                             |                                                                                             
AACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACC                                                                                               <  SRR4302540.5741279/94‑1 (MQ=60)
  CAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGT                                                                                             <  SRR4302540.9271322/94‑1 (MQ=60)
  CAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGT                                                                                             <  SRR4302540.1580012/94‑1 (MQ=60)
     CAGAACGGCTGGCAGGAACGTCTGCAGTCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTA                                                                                          <  SRR4302540.6359356/94‑1 (MQ=60)
     CAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTA                                                                                          >  SRR4302540.2150545/1‑94 (MQ=60)
     CAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTA                                                                                          >  SRR4302540.4469830/1‑94 (MQ=60)
      AGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTCCGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAG                                                                                         >  SRR4302540.5173711/1‑94 (MQ=60)
      AGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAG                                                                                         <  SRR4302540.4653109/94‑1 (MQ=60)
      AGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAG                                                                                         >  SRR4302540.2827529/1‑94 (MQ=60)
      AGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAG                                                                                         <  SRR4302540.3222072/94‑1 (MQ=60)
      AGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAG                                                                                         <  SRR4302540.5210896/94‑1 (MQ=60)
      AGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAG                                                                                         <  SRR4302540.7004338/94‑1 (MQ=60)
      AGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAG                                                                                         <  SRR4302540.7745456/94‑1 (MQ=60)
      AGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAG                                                                                         <  SRR4302540.7412990/94‑1 (MQ=60)
      AGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGGCTGATTACACTTCATCTTCCGCGTAATTTCTTTACCGTGTAG                                                                                         >  SRR4302540.4247784/1‑94 (MQ=60)
       GAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGG                                                                                        <  SRR4302540.3026797/94‑1 (MQ=60)
        AACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGC                                                                                       >  SRR4302540.6602666/1‑94 (MQ=60)
         ACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTTGGCC                                                                                      >  SRR4302540.7606839/1‑94 (MQ=60)
         ACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCC                                                                                      <  SRR4302540.433769/94‑1 (MQ=60)
          CGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCA                                                                                     >  SRR4302540.8371126/1‑94 (MQ=60)
          CGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCA                                                                                     <  SRR4302540.1036413/94‑1 (MQ=60)
           GGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAA                                                                                    <  SRR4302540.9858632/94‑1 (MQ=60)
           GGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGGCGGATGGAAGACGGCTGATTACGCATCATCTTCCGCGTAATTTCTTTACAGTGTACGCGAA                                                                                    >  SRR4302540.1620853/1‑94 (MQ=60)
            GCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTNATTTCTTTACCGTGTAGGCCAAC                                                                                   >  SRR4302540.4287938/1‑94 (MQ=60)
             CTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACA                                                                                  <  SRR4302540.2090777/94‑1 (MQ=60)
             CTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACA                                                                                  <  SRR4302540.4701228/94‑1 (MQ=60)
             CTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACA                                                                                  <  SRR4302540.4701229/94‑1 (MQ=60)
              TGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAG                                                                                 <  SRR4302540.2446540/94‑1 (MQ=60)
              TGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAG                                                                                 <  SRR4302540.3314656/94‑1 (MQ=60)
              TGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAG                                                                                 >  SRR4302540.3848394/1‑94 (MQ=60)
              TGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAG                                                                                 <  SRR4302540.3024192/94‑1 (MQ=60)
              TGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGCTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAG                                                                                 <  SRR4302540.3314655/94‑1 (MQ=60)
               GGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGG                                                                                >  SRR4302540.444456/1‑94 (MQ=60)
                GCAGGAGCGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGT                                                                               <  SRR4302540.7789445/94‑1 (MQ=60)
                GCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGT                                                                               <  SRR4302540.6050473/94‑1 (MQ=60)
                  AGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAA                                                                             >  SRR4302540.2579910/1‑94 (MQ=60)
                  AGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAA                                                                             >  SRR4302540.6895818/1‑94 (MQ=60)
                  AGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAA                                                                             <  SRR4302540.287190/94‑1 (MQ=60)
                   GGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAAC                                                                            >  SRR4302540.9392899/1‑94 (MQ=60)
                    GAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACG                                                                           <  SRR4302540.459899/94‑1 (MQ=60)
                    GAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACG                                                                           >  SRR4302540.8435072/1‑94 (MQ=60)
                     AACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGC                                                                          >  SRR4302540.2409934/1‑94 (MQ=60)
                      ACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCA                                                                         <  SRR4302540.7975981/94‑1 (MQ=60)
                       CGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAG                                                                        <  SRR4302540.2610274/94‑1 (MQ=60)
                        GTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGA                                                                       >  SRR4302540.8412639/1‑94 (MQ=60)
                        GTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGA                                                                       >  SRR4302540.8665124/1‑94 (MQ=60)
                        GTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGA                                                                       >  SRR4302540.1551877/1‑94 (MQ=60)
                          CTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAA                                                                     >  SRR4302540.6549208/1‑94 (MQ=60)
                          CTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAA                                                                     >  SRR4302540.8505864/1‑94 (MQ=60)
                             CAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAA                                                                  <  SRR4302540.1544627/94‑1 (MQ=60)
                              AGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAG                                                                 <  SRR4302540.730300/94‑1 (MQ=60)
                              AGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAG                                                                 <  SRR4302540.6393528/94‑1 (MQ=60)
                              AGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAG                                                                 >  SRR4302540.5618779/1‑94 (MQ=60)
                               GGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGG                                                                >  SRR4302540.6200747/1‑94 (MQ=60)
                                 CCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCA                                                              >  SRR4302540.9559300/1‑94 (MQ=60)
                                  CCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCAC                                                             <  SRR4302540.9395575/94‑1 (MQ=60)
                                  CCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCAC                                                             <  SRR4302540.5435202/94‑1 (MQ=60)
                                  CCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCAC                                                             >  SRR4302540.5622206/1‑94 (MQ=60)
                                   CTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACC                                                            >  SRR4302540.4664213/1‑94 (MQ=60)
                                   CTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACC                                                            >  SRR4302540.1227218/1‑94 (MQ=60)
                                    CGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCT                                                           <  SRR4302540.64885/94‑1 (MQ=60)
                                     GCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTT                                                          <  SRR4302540.5762543/94‑1 (MQ=60)
                                     GCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTT                                                          <  SRR4302540.7831041/94‑1 (MQ=60)
                                     GCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTT                                                          <  SRR4302540.6812532/94‑1 (MQ=60)
                                      GTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTG                                                         <  SRR4302540.7436910/94‑1 (MQ=60)
                                      CTGTGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTG                                                         <  SRR4302540.9950822/94‑1 (MQ=60)
                                       TGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGC                                                        <  SRR4302540.9599480/94‑1 (MQ=60)
                                       TGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGC                                                        >  SRR4302540.1465140/1‑94 (MQ=60)
                                       TGGGTCGCATGGAAGAGGTCTGATTACGCTTCATATTCAGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGC                                                        >  SRR4302540.1465138/1‑94 (MQ=60)
                                        GGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCG                                                       <  SRR4302540.1974105/94‑1 (MQ=60)
                                         GGTCGCATGGACGAGGTCTGATTACGCTTCATCTTCCGCGTCATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGG                                                      <  SRR4302540.9069406/94‑1 (MQ=60)
                                          GTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGT                                                     <  SRR4302540.8717098/94‑1 (MQ=60)
                                           TCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTG                                                    <  SRR4302540.1349823/94‑1 (MQ=60)
                                           TCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTG                                                    <  SRR4302540.1667165/94‑1 (MQ=60)
                                           TCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTG                                                    <  SRR4302540.5960143/94‑1 (MQ=60)
                                            CGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGC                                                   >  SRR4302540.6249305/1‑94 (MQ=60)
                                            CGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGC                                                   <  SRR4302540.9701030/94‑1 (MQ=60)
                                            CGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCCTGCGGTGC                                                   >  SRR4302540.7794163/1‑94 (MQ=60)
                                             GCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCACCAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCC                                                  >  SRR4302540.3524510/1‑94 (MQ=60)
                                             GCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCC                                                  <  SRR4302540.6721434/94‑1 (MQ=60)
                                             GCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCC                                                  <  SRR4302540.6721435/94‑1 (MQ=60)
                                              gaggaaggggttttatttcgtttttttctccgggGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCT                                                 <  SRR4302540.6158594/60‑1 (MQ=60)
                                              CATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCT                                                 <  SRR4302540.6396963/94‑1 (MQ=60)
                                               ATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGCAAAAAGGCACCTTGCGGTGCCTT                                                >  SRR4302540.3073043/1‑94 (MQ=60)
                                               ATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTT                                                >  SRR4302540.8471990/1‑94 (MQ=60)
                                                 GGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTC                                              >  SRR4302540.8066297/1‑94 (MQ=60)
                                                 GGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGCGCCTTTT                                              >  SRR4302540.7818067/1‑94 (MQ=60)
                                                  GAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCT                                             >  SRR4302540.6290777/1‑94 (MQ=60)
                                                  GAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCT                                             >  SRR4302540.1678412/1‑94 (MQ=60)
                                                   AAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTT                                            <  SRR4302540.7449087/94‑1 (MQ=60)
                                                   AAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTT                                            <  SRR4302540.5750172/94‑1 (MQ=60)
                                                    AGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTA                                           <  SRR4302540.4350199/94‑1 (MQ=60)
                                                    AGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTA                                           <  SRR4302540.3217785/94‑1 (MQ=60)
                                                    AGAGGTCTGATTACGCTTCATCTTCCGCGGAATTTCTTTACCGTGTAGGCCAAGAGGTAACGCGGAAGAAAGGCAGCGTGGGGTGCCTTTCctc                                           >  SRR4302540.690214/1‑91 (MQ=59)
                                                     GAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTAT                                          >  SRR4302540.5081687/1‑94 (MQ=60)
                                                     GAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTAT                                          <  SRR4302540.7483119/94‑1 (MQ=60)
                                                     GAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGGGCCTTTCTTAT                                          >  SRR4302540.2638625/1‑94 (MQ=60)
                                                      agtcctgtttccgtTTCATCTTCCGCTAAATTTC‑TTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCA                                        <  SRR4302540.7565214/80‑1 (MQ=60)
                                                      AGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTTCGGTGCGTTTCTTATT                                         >  SRR4302540.7144707/1‑94 (MQ=60)
                                                      AGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATC                                         <  SRR4302540.4425958/94‑1 (MQ=60)
                                                      AGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATC                                         >  SRR4302540.5434692/1‑94 (MQ=60)
                                                       GGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCA                                        <  SRR4302540.7565213/94‑1 (MQ=60)
                                                        GTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCAT                                       <  SRR4302540.10083950/94‑1 (MQ=60)
                                                        GTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCAT                                       >  SRR4302540.8586610/1‑94 (MQ=60)
                                                         TCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATT                                      <  SRR4302540.9575380/94‑1 (MQ=60)
                                                         TCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATT                                      <  SRR4302540.9177404/94‑1 (MQ=60)
                                                         TCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATT                                      >  SRR4302540.5113506/1‑94 (MQ=60)
                                                          CTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTC                                     >  SRR4302540.2849375/1‑94 (MQ=60)
                                                          CTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTC                                     <  SRR4302540.9799465/94‑1 (MQ=60)
                                                          CTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTC                                     <  SRR4302540.6232546/94‑1 (MQ=60)
                                                          CTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTC                                     <  SRR4302540.5562017/94‑1 (MQ=60)
                                                          CTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCCTGCGGGGCCTTTCTTATCATTC                                     >  SRR4302540.4231034/1‑94 (MQ=60)
                                                           TGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCA                                    <  SRR4302540.5503811/94‑1 (MQ=60)
                                                           TGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCA                                    <  SRR4302540.2155806/94‑1 (MQ=60)
                                                           TGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCA                                    >  SRR4302540.1328083/1‑94 (MQ=60)
                                                            GATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAA                                   >  SRR4302540.8596399/1‑94 (MQ=60)
                                                            GATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAA                                   >  SRR4302540.1771943/1‑94 (MQ=60)
                                                            GATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAA                                   >  SRR4302540.4746485/1‑94 (MQ=60)
                                                            GATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCATTTCTTATCATTCAA                                   >  SRR4302540.4746487/1‑94 (MQ=60)
                                                            GATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAAGCACCCTGCGGTGgccttttttttatttta                                   >  SRR4302540.3745951/1‑77 (MQ=60)
                                                            GATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGGAGGCCTACAGGTAACGCAGAAAAAAGGCACCTTGCGGGGGCTTTCTTATCcttcgc                                   >  SRR4302540.9568689/1‑88 (MQ=60)
                                                             ATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAcgcaaccttggggggccttctttttcttttaa                                  >  SRR4302540.3785149/1‑62 (MQ=60)
                                                             ATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCTGTGCCTTTATTATCctcacat                                  >  SRR4302540.788792/1‑87 (MQ=60)
                                                             ATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAAT                                  >  SRR4302540.788793/1‑94 (MQ=60)
                                                              TTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATA                                 <  SRR4302540.1811584/94‑1 (MQ=60)
                                                              TTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATA                                 <  SRR4302540.446755/94‑1 (MQ=60)
                                                               TACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATAT                                <  SRR4302540.9793001/94‑1 (MQ=60)
                                                               TACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATAT                                <  SRR4302540.6736331/94‑1 (MQ=60)
                                                                 CGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCC                              >  SRR4302540.1629742/1‑94 (MQ=60)
                                                                 CGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCC                              >  SRR4302540.8212027/1‑94 (MQ=60)
                                                                  GCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCA                             <  SRR4302540.4014168/94‑1 (MQ=60)
                                                                  GCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCA                             >  SRR4302540.3583758/1‑94 (MQ=60)
                                                                  GCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCA                             >  SRR4302540.401360/1‑94 (MQ=60)
                                                                  GCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCA                             <  SRR4302540.7751262/94‑1 (MQ=60)
                                                                  GCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCA                             <  SRR4302540.5120718/94‑1 (MQ=60)
                                                                  GCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCCTGCGGTGCCTTTCTTTTCATTCTATATTCA                             >  SRR4302540.2089633/1‑94 (MQ=60)
                                                                   CTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAG                            <  SRR4302540.5608716/94‑1 (MQ=60)
                                                                    TTCATCTTCCTCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGC                           <  SRR4302540.4581582/94‑1 (MQ=60)
                                                                    TTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATACAGC                           >  SRR4302540.3677323/1‑94 (MQ=60)
                                                                      CATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGG                         >  SRR4302540.8836872/1‑94 (MQ=60)
                                                                      CATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGG                         <  SRR4302540.10157441/94‑1 (MQ=60)
                                                                       ATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGA                        >  SRR4302540.8358304/1‑94 (MQ=60)
                                                                       ATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGA                        <  SRR4302540.7425647/94‑1 (MQ=60)
                                                                       ATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGA                        <  SRR4302540.3525579/94‑1 (MQ=60)
                                                                       ATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGA                        >  SRR4302540.1741480/1‑94 (MQ=60)
                                                                        TCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAT                       <  SRR4302540.7147290/94‑1 (MQ=60)
                                                                        TCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAT                       <  SRR4302540.7147292/94‑1 (MQ=60)
                                                                        TCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAT                       <  SRR4302540.3962766/94‑1 (MQ=60)
                                                                        TCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAT                       >  SRR4302540.8453743/1‑94 (MQ=60)
                                                                         CTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATC                      >  SRR4302540.6991100/1‑94 (MQ=60)
                                                                         CTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATC                      <  SRR4302540.6745479/94‑1 (MQ=60)
                                                                         CTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATC                      <  SRR4302540.9731178/94‑1 (MQ=60)
                                                                          TTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCT                     <  SRR4302540.6505860/94‑1 (MQ=60)
                                                                          TTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCCTGCGGTGgcttttttattatttaataatcatccgatgt                     >  SRR4302540.5517170/1‑63 (MQ=60)
                                                                           TCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTT                    <  SRR4302540.5956487/94‑1 (MQ=60)
                                                                           TCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTT                    <  SRR4302540.2961785/94‑1 (MQ=60)
                                                                            CCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTC                   >  SRR4302540.10056609/1‑94 (MQ=60)
                                                                             CGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCT                  >  SRR4302540.2735295/1‑94 (MQ=60)
                                                                              GCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTG                 <  SRR4302540.1563548/94‑1 (MQ=60)
                                                                                GTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGGACCCTGCGGTGCCCTTCTTATTATTCAATATTTAGCGGATCTTCTTAA               >  SRR4302540.5528514/1‑94 (MQ=60)
                                                                                GTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCaacggattttcttaaa               >  SRR4302540.1456046/1‑78 (MQ=60)
                                                                                GTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAA               >  SRR4302540.10066743/1‑94 (MQ=60)
                                                                                GTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAA               >  SRR4302540.2687502/1‑94 (MQ=60)
                                                                                GTAAGTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAA               <  SRR4302540.7065066/94‑1 (MQ=60)
                                                                                 TAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAA              <  SRR4302540.4167259/94‑1 (MQ=60)
                                                                                 TAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAA              <  SRR4302540.8524966/94‑1 (MQ=60)
                                                                                  TATTTCTTTTCCGTGGAGGCCAACAGGTAACGCAGAAAAAAGGCCCCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAG             <  SRR4302540.1427392/94‑1 (MQ=60)
                                                                                  AATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCAgcctgccggggctttcttttcatttaattatcttcgggatttttgtaaa             >  SRR4302540.3839357/1‑45 (MQ=60)
                                                                                  AATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAG             <  SRR4302540.3153883/94‑1 (MQ=60)
                                                                                   ATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCCGCGGATCTTCTGAAAGA            >  SRR4302540.8272270/1‑94 (MQ=60)
                                                                                   ATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGA            >  SRR4302540.8272272/1‑94 (MQ=60)
                                                                                   ATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGA            >  SRR4302540.579175/1‑94 (MQ=60)
                                                                                    TTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAA           <  SRR4302540.7067734/94‑1 (MQ=60)
                                                                                    TTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAA           >  SRR4302540.5465191/1‑94 (MQ=60)
                                                                                     TTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAAT          <  SRR4302540.4986384/94‑1 (MQ=60)
                                                                                     TTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAAT          <  SRR4302540.4425007/94‑1 (MQ=60)
                                                                                     TTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAAT          <  SRR4302540.7025749/94‑1 (MQ=60)
                                                                                     TTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAAT          <  SRR4302540.1853268/94‑1 (MQ=60)
                                                                                       CTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGtggaggcttttttatcattccatattcacgcgaagttctgaaagaatta        >  SRR4302540.9004437/1‑45 (MQ=60)
                                                                                       CTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAA        <  SRR4302540.6847611/94‑1 (MQ=60)
                                                                                       CTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAA        >  SRR4302540.4836880/1‑94 (MQ=60)
                                                                                       CTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAA        <  SRR4302540.3972602/94‑1 (MQ=60)
                                                                                       CGTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAA        <  SRR4302540.1893711/94‑1 (MQ=60)
                                                                                        TTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAAT       >  SRR4302540.3354172/1‑94 (MQ=60)
                                                                                        TTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAAT       >  SRR4302540.2896995/1‑94 (MQ=60)
                                                                                        TTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAAT       <  SRR4302540.1639344/94‑1 (MQ=60)
                                                                                        TTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAAT       <  SRR4302540.6543432/94‑1 (MQ=60)
                                                                                         TTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGGGCCTTTCTTATCATTCAATATCCAGCGGCTCTTCTGAAAAAAAAATC      >  SRR4302540.2561773/1‑94 (MQ=60)
                                                                                          TACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGgcccctgtggggggctttttttttttttatattcaatcggtatttttttaggataatac     >  SRR4302540.5504149/1‑35 (MQ=60)
                                                                                          TACCGTGGAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCC     <  SRR4302540.952711/94‑1 (MQ=60)
                                                                                           ACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCC    <  SRR4302540.10143348/94‑1 (MQ=60)
                                                                                           ACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCGTTCTTATCATTCAAGATCCAGCGGATCTTCTGAAAGAATAATCCC    <  SRR4302540.9209031/94‑1 (MQ=60)
                                                                                            CCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCG   >  SRR4302540.9523257/1‑94 (MQ=60)
                                                                                            CCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCG   <  SRR4302540.8144894/94‑1 (MQ=60)
                                                                                            CCGTGTAGGCCAACAGGGAACGCAGAAAAAAGGCACCTGGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCG   <  SRR4302540.3333583/94‑1 (MQ=60)
                                                                                             CGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTTCGGGGCCTTTCTTATCCTTCAATATCCACCGGGTCTTCTGAAAGAATAAACCCCG  >  SRR4302540.2364691/1‑94 (MQ=60)
                                                                                             CGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGG  <  SRR4302540.9130837/94‑1 (MQ=60)
                                                                                             CGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGG  <  SRR4302540.4923896/94‑1 (MQ=60)
                                                                                             CGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGG  <  SRR4302540.5885786/94‑1 (MQ=60)
                                                                                             CGTGTAGGCCAACAGGTAACGCAGAAAAAACGCACCcttcgggggccttcttctacttcaataaacagggggtcctcttaaagaaaaatccccg  >  SRR4302540.2364690/1‑36 (MQ=58)
                                                                                             |                                                                                             
AACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGG  >  NC_000913/4116279‑4116465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑