Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,116,372 T→C intergenic (+32/+53) yiiU → / ← menG hypothetical protein/MenG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,116,3720TC100.0% 799.5 / NA 245intergenic (+32/+53)yiiU/menGhypothetical protein/MenG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (110/135);  total (110/135)

Only 100 of 255 total aligned reads displayed.

AGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATC  >  NC_000913/4116282‑4116461
                                                                                          |                                                                                         
  cagAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCgtgt                                                                                       <  1:1715059/93‑1 (MQ=255)
   agAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTa                                                                                      <  1:986059/93‑1 (MQ=255)
    gAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAg                                                                                     >  2:1714887/1‑93 (MQ=255)
     aaCGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAgg                                                                                    >  1:2020661/1‑93 (MQ=255)
         gCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCaa                                                                                >  1:118773/1‑93 (MQ=255)
         gCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCaa                                                                                >  2:4248263/1‑93 (MQ=255)
          cTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAAc                                                                               <  1:1735280/93‑1 (MQ=255)
             gCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAgg                                                                            <  2:2574519/93‑1 (MQ=255)
             gCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAgg                                                                            <  2:3331992/93‑1 (MQ=255)
                ggAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTaa                                                                         >  1:6098940/1‑93 (MQ=255)
                ggAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTaa                                                                         >  2:41135/1‑93 (MQ=255)
                  aaCGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACg                                                                       >  2:5520445/1‑93 (MQ=255)
                  aaCGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACg                                                                       >  2:2149796/1‑93 (MQ=255)
                  aaCGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACg                                                                       <  1:1920358/93‑1 (MQ=255)
                   aCGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGc                                                                      <  2:5665593/93‑1 (MQ=255)
                   aCGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGc                                                                      <  1:2739127/93‑1 (MQ=255)
                   aCGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGc                                                                      <  2:5148233/93‑1 (MQ=255)
                   aCGTCTACAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGc                                                                      >  2:4445485/1‑93 (MQ=255)
                     gTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAg                                                                    <  1:5250343/93‑1 (MQ=255)
                      tCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGa                                                                   <  2:2239024/93‑1 (MQ=255)
                      tCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGa                                                                   >  1:415930/1‑93 (MQ=255)
                        tGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGaaa                                                                 >  1:2337203/1‑93 (MQ=255)
                         gCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGaaaa                                                                >  1:2974453/1‑93 (MQ=255)
                            ggCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAg                                                             >  2:623956/1‑93 (MQ=255)
                            ggCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAg                                                             >  2:2713819/1‑93 (MQ=255)
                             gCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAgg                                                            <  2:6071662/93‑1 (MQ=255)
                             gCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAgg                                                            <  2:5719872/93‑1 (MQ=255)
                              cccTGCTGCGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGc                                                           <  2:1111400/93‑1 (MQ=255)
                               ccTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCa                                                          >  1:4660548/1‑93 (MQ=255)
                                ctgctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCAc                                                         <  2:5290572/93‑1 (MQ=255)
                                 tgctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAACAAGGCAcc                                                        >  2:3136558/1‑93 (MQ=255)
                                 tgctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCAcc                                                        <  1:632233/93‑1 (MQ=255)
                                 tgctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCAcc                                                        <  1:1534168/93‑1 (MQ=255)
                                  gctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCt                                                       <  2:3087489/93‑1 (MQ=255)
                                  gctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCt                                                       <  2:4999525/93‑1 (MQ=255)
                                   ctgGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCtt                                                      <  2:5854377/93‑1 (MQ=255)
                                      ggTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCg                                                   >  2:1573522/1‑93 (MQ=255)
                                       gTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCgg                                                  <  1:1053689/93‑1 (MQ=255)
                                       gTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCgg                                                  <  1:5457375/93‑1 (MQ=255)
                                            aTGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAACGCACCTTGCGGTGGCt                                             >  2:1943852/1‑93 (MQ=255)
                                             tGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCtt                                            >  2:2634384/1‑93 (MQ=255)
                                               gAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTc                                          >  2:2050074/1‑93 (MQ=255)
                                               gAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTc                                          <  1:4202326/93‑1 (MQ=255)
                                               gAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTc                                          <  1:2218801/93‑1 (MQ=255)
                                               gAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTc                                          >  1:453580/1‑93 (MQ=255)
                                                aaGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCt                                         <  1:4727795/93‑1 (MQ=255)
                                                aaGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCCTGCGGGGGCTTTCt                                         >  2:5393487/1‑93 (MQ=255)
                                                 agagGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCtt                                        >  1:2419692/1‑93 (MQ=255)
                                                  gagGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTa                                       <  1:258400/93‑1 (MQ=255)
                                                    ggTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATc                                     >  2:1795556/1‑93 (MQ=255)
                                                    ggTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGACAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATc                                     >  2:1740406/1‑93 (MQ=255)
                                                     gTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCa                                    <  1:5906963/93‑1 (MQ=255)
                                                       cTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCAtt                                  >  2:1884397/1‑93 (MQ=255)
                                                        tGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTc                                 >  2:2488127/1‑93 (MQ=255)
                                                         gATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCa                                <  2:4628240/93‑1 (MQ=255)
                                                          aTTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCaa                               >  1:6235060/1‑93 (MQ=255)
                                                           ttACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAat                              <  2:3970563/93‑1 (MQ=255)
                                                           ttACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAat                              <  2:2910349/93‑1 (MQ=255)
                                                           ttACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAat                              >  1:3738030/1‑93 (MQ=255)
                                                            tACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAata                             <  1:4461927/93‑1 (MQ=255)
                                                            tACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAata                             <  1:3526379/93‑1 (MQ=255)
                                                              cGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATc                           <  2:3894870/93‑1 (MQ=255)
                                                                cTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCa                         <  1:3079944/93‑1 (MQ=255)
                                                                 ttCATCTTCCGTGTAATTTCTTTGCTGTGTAGCCCAACAGGTAACGCAGAAAAAAGGCACCTCGCGGTGGGTTTCTTATCAATCAATATCCAg                        <  2:2357225/93‑1 (MQ=255)
                                                                 ttCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCCTCCGGGGCCTTTCTTCTTATTTAatattcca                        >  1:3418669/1‑89 (MQ=255)
                                                                  tCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGc                       <  2:2306797/93‑1 (MQ=255)
                                                                   cATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCg                      <  1:5036943/93‑1 (MQ=255)
                                                                   cATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCCTGCGGTGCCTTTCTTTTTATTCAATATTCAGCg                      >  2:5547728/1‑93 (MQ=255)
                                                                    aTCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCgg                     <  1:2787913/93‑1 (MQ=255)
                                                                    aTCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCgg                     >  2:666407/1‑93 (MQ=255)
                                                                    aTCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAACgg                     >  1:3956668/1‑93 (MQ=255)
                                                                    aTCTGCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCgg                     <  1:2910577/93‑1 (MQ=255)
                                                                     tCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTTAATATCCAGCGGa                    >  2:3656394/1‑93 (MQ=255)
                                                                      cTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAt                   <  1:6036921/93‑1 (MQ=255)
                                                                      cTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAt                   <  1:3234495/93‑1 (MQ=255)
                                                                      cTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAt                   <  1:5121749/93‑1 (MQ=255)
                                                                        tCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtct                 >  2:2101558/1‑93 (MQ=255)
                                                                         ccGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtctt                <  2:774251/93‑1 (MQ=255)
                                                                           gcgTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGAtcttct              >  1:5688845/1‑93 (MQ=255)
                                                                              tAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGaa           >  1:4941737/1‑93 (MQ=255)
                                                                              tAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCCTTGGGGGGCTTTCTTTTTATTCAATATCCAACGGATCTTCTGaa           >  1:75888/1‑93 (MQ=255)
                                                                               aaTTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGaaa          <  1:824989/93‑1 (MQ=255)
                                                                               aaTTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGaaa          <  1:2173867/93‑1 (MQ=255)
                                                                                 tttCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATTCAGCGGATCTTCTGAAAGa        >  1:2641344/1‑93 (MQ=255)
                                                                                 tttCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGa        >  2:5620477/1‑93 (MQ=255)
                                                                                  ttCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaa       <  2:3191008/93‑1 (MQ=255)
                                                                                  ttCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaa       <  1:4045292/93‑1 (MQ=255)
                                                                                  ttCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaa       <  2:1641640/93‑1 (MQ=255)
                                                                                   tCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaat      <  2:2938938/93‑1 (MQ=255)
                                                                                    ctgtACCGTGTAGACCAACAGGTGACGCAGAAACAAGGCACCTTGCGGTGCGTTGCATATCATCCAATATCCAGCGGATCATCTGAAAAaata     <  2:1458518/90‑1 (MQ=255)
                                                                                    cTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaata     >  2:3642001/1‑93 (MQ=255)
                                                                                    cTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaata     <  1:147162/93‑1 (MQ=255)
                                                                                    cTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaata     <  1:232725/93‑1 (MQ=255)
                                                                                     tttACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCCGAAAGaataa    <  2:3523911/93‑1 (MQ=255)
                                                                                      ttACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaataat   <  2:922830/93‑1 (MQ=255)
                                                                                      ttACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGaataat   <  2:1219489/93‑1 (MQ=255)
                                                                                       tACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATc  >  2:602046/1‑93 (MQ=255)
                                                                                       tACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATc  <  2:2006093/93‑1 (MQ=255)
                                                                                       tACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATc  <  1:5671886/93‑1 (MQ=255)
                                                                                       tACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATc  >  2:4698690/1‑93 (MQ=255)
                                                                                          |                                                                                         
AGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATC  >  NC_000913/4116282‑4116461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGG  >  NC_000913/4116279‑4116465
                                                                                             |                                                                                             
AACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACC                                                                                               <  SRR4302533.3217571/94‑1 (MQ=60)
AACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACC                                                                                               <  SRR4302533.2800153/94‑1 (MQ=60)
  CAGCAGAACGGCTGGCAGGAACGTCTGCTGGCCCTGCTGGGGCGCATGGAAGAGGGCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGT                                                                                             >  SRR4302533.1875875/1‑94 (MQ=60)
  CAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGT                                                                                             <  SRR4302533.773965/94‑1 (MQ=60)
     CAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTA                                                                                          <  SRR4302533.5735012/94‑1 (MQ=60)
     CAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTA                                                                                          <  SRR4302533.1725458/94‑1 (MQ=60)
     CAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGGCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTA                                                                                          >  SRR4302533.3542607/1‑94 (MQ=60)
      AGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAG                                                                                         <  SRR4302533.991829/94‑1 (MQ=60)
      AGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAG                                                                                         <  SRR4302533.3157416/94‑1 (MQ=60)
      AGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAG                                                                                         <  SRR4302533.3545124/94‑1 (MQ=60)
       GAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGG                                                                                        >  SRR4302533.2033104/1‑94 (MQ=60)
           GGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAA                                                                                    >  SRR4302533.119527/1‑94 (MQ=60)
             CTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACA                                                                                  <  SRR4302533.1745806/94‑1 (MQ=60)
             CTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACA                                                                                  >  SRR4302533.4223931/1‑94 (MQ=60)
              TGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGGCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAG                                                                                 >  SRR4302533.2849091/1‑94 (MQ=60)
                 CAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTATAGGCCAACAGGTA                                                                              >  SRR4302533.3658740/1‑94 (MQ=60)
                  AGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAA                                                                             >  SRR4302533.6135770/1‑94 (MQ=60)
                  AGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAA                                                                             <  SRR4302533.2344320/94‑1 (MQ=60)
                     AACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGC                                                                          >  SRR4302533.1631320/1‑94 (MQ=60)
                     AACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGC                                                                          <  SRR4302533.1932080/94‑1 (MQ=60)
                      ACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCA                                                                         <  SRR4302533.2755923/94‑1 (MQ=60)
                      ACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCA                                                                         <  SRR4302533.2254737/94‑1 (MQ=60)
                        GTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGA                                                                       >  SRR4302533.418428/1‑94 (MQ=60)
                        GTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGA                                                                       <  SRR4302533.5281896/94‑1 (MQ=60)
                          CTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAA                                                                     >  SRR4302533.5059373/1‑94 (MQ=60)
                          CTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAA                                                                     >  SRR4302533.2351669/1‑94 (MQ=60)
                           TGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAA                                                                    >  SRR4302533.2992653/1‑94 (MQ=60)
                              AGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAG                                                                 >  SRR4302533.2185933/1‑94 (MQ=60)
                                 CCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCA                                                              >  SRR4302533.4688791/1‑94 (MQ=60)
                                   CTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAagaaac                                                            >  SRR4302533.5381094/1‑88 (MQ=60)
                                   CTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACC                                                            >  SRR4302533.2071054/1‑94 (MQ=60)
                                    TGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCT                                                           >  SRR4302533.324503/1‑94 (MQ=60)
                                    TGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCT                                                           <  SRR4302533.2674610/94‑1 (MQ=60)
                                    TGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCT                                                           <  SRR4302533.1543384/94‑1 (MQ=60)
                                    TGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCT                                                           <  SRR4302533.635954/94‑1 (MQ=60)
                                      CTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTG                                                         <  SRR4302533.5232740/94‑1 (MQ=60)
                                       TGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGC                                                        <  SRR4302533.3436630/94‑1 (MQ=60)
                                        TGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCG                                                       <  SRR4302533.5711005/94‑1 (MQ=60)
                                        GGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCG                                                       >  SRR4302533.4922698/1‑94 (MQ=60)
                                         GGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGG                                                      <  SRR4302533.2343329/94‑1 (MQ=60)
                                          GTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGT                                                     <  SRR4302533.1483401/94‑1 (MQ=60)
                                          GTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGT                                                     <  SRR4302533.5490255/94‑1 (MQ=60)
                                          GTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGT                                                     <  SRR4302533.1059895/94‑1 (MQ=60)
                                              CATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCT                                                 <  SRR4302533.5971593/94‑1 (MQ=60)
                                               ATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTT                                                <  SRR4302533.4670389/94‑1 (MQ=60)
                                                TGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTT                                               <  SRR4302533.241743/94‑1 (MQ=60)
                                                TGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTT                                               >  SRR4302533.1407219/1‑94 (MQ=60)
                                                 GGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTC                                              >  SRR4302533.4289408/1‑94 (MQ=60)
                                                 GGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTC                                              >  SRR4302533.456291/1‑94 (MQ=60)
                                                 GGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTC                                              <  SRR4302533.2586130/94‑1 (MQ=60)
                                                 GGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTC                                              <  SRR4302533.877495/94‑1 (MQ=60)
                                                  GAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCT                                             <  SRR4302533.4227749/94‑1 (MQ=60)
                                                  GAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCT                                             >  SRR4302533.3770581/1‑94 (MQ=60)
                                                  GAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCT                                             <  SRR4302533.2232500/94‑1 (MQ=60)
                                                  GAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGGAACGCAGAAAAAAGGAACCTTGCGGTGCCTTTCT                                             >  SRR4302533.4076642/1‑94 (MQ=60)
                                                   AAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTT                                            >  SRR4302533.2434637/1‑94 (MQ=60)
                                                   AAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTT                                            <  SRR4302533.4756456/94‑1 (MQ=60)
                                                    AGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTA                                           <  SRR4302533.2241697/94‑1 (MQ=60)
                                                    AGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTA                                           >  SRR4302533.769345/1‑94 (MQ=60)
                                                    AGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTA                                           <  SRR4302533.1598058/94‑1 (MQ=60)
                                                     GAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTAT                                          <  SRR4302533.6035719/94‑1 (MQ=60)
                                                     GAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTAT                                          <  SRR4302533.259973/94‑1 (MQ=60)
                                                      AGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATC                                         >  SRR4302533.5119201/1‑94 (MQ=60)
                                                       GGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCA                                        <  SRR4302533.1808788/94‑1 (MQ=60)
                                                       GGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCA                                        <  SRR4302533.4331875/94‑1 (MQ=60)
                                                        GTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCAT                                       <  SRR4302533.5942633/94‑1 (MQ=60)
                                                          CTGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTC                                     <  SRR4302533.935692/94‑1 (MQ=60)
                                                           TGATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCA                                    <  SRR4302533.4524280/94‑1 (MQ=60)
                                                            GATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAA                                   >  SRR4302533.6272392/1‑94 (MQ=60)
                                                             ATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAAT                                  >  SRR4302533.3760957/1‑94 (MQ=60)
                                                             ATTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGCAACGCAGAAAAAAGGCAacgtgagggctgcttctttttattctat                                  >  SRR4302533.2239713/1‑66 (MQ=60)
                                                              TTACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATA                                 <  SRR4302533.6272762/94‑1 (MQ=60)
                                                               TACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATAT                                <  SRR4302533.3548109/94‑1 (MQ=60)
                                                               TACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATAT                                <  SRR4302533.4488914/94‑1 (MQ=60)
                                                                ACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATC                               >  SRR4302533.2786392/1‑94 (MQ=60)
                                                                ACGCTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGGGCCTTTCTTATCCTTCAATATC                               >  SRR4302533.2695450/1‑94 (MQ=60)
                                                                   CTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAG                            >  SRR4302533.753771/1‑94 (MQ=60)
                                                                   CTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAG                            >  SRR4302533.4972050/1‑94 (MQ=60)
                                                                   CTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAG                            <  SRR4302533.3098842/94‑1 (MQ=60)
                                                                   CTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAG                            <  SRR4302533.5376334/94‑1 (MQ=60)
                                                                   CTTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCCTCCGGGGCCTTTCTTcttatttaatattcca                            >  SRR4302533.3439792/1‑78 (MQ=60)
                                                                    TTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGC                           >  SRR4302533.3073010/1‑94 (MQ=60)
                                                                    TTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGC                           <  SRR4302533.219484/94‑1 (MQ=60)
                                                                    TTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGC                           <  SRR4302533.5153123/94‑1 (MQ=60)
                                                                    TTCATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAAGCACCTTGCGGCGGCTTTCTTTTTATTCAATATtcaac                           >  SRR4302533.4585424/1‑89 (MQ=60)
                                                                      CATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGG                         <  SRR4302533.5067445/94‑1 (MQ=60)
                                                                      CATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAACGG                         >  SRR4302533.3980785/1‑94 (MQ=60)
                                                                       ATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGA                        <  SRR4302533.2805042/94‑1 (MQ=60)
                                                                       ATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGA                        >  SRR4302533.5886996/1‑94 (MQ=60)
                                                                       ATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGA                        <  SRR4302533.3938796/94‑1 (MQ=60)
                                                                       ATCTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGA                        <  SRR4302533.3981944/94‑1 (MQ=60)
                                                                       ATCTGCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGA                        <  SRR4302533.2928445/94‑1 (MQ=60)
                                                                         CTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGTGGTGGCTTTTTTTTCATTTAATATCaagccggtg                      >  SRR4302533.1192449/1‑85 (MQ=60)
                                                                         CTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATC                      <  SRR4302533.5152693/94‑1 (MQ=60)
                                                                         CTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATC                      <  SRR4302533.3254426/94‑1 (MQ=60)
                                                                         CTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATC                      >  SRR4302533.2963377/1‑94 (MQ=60)
                                                                         CTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATC                      >  SRR4302533.6156148/1‑94 (MQ=60)
                                                                         CTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATC                      <  SRR4302533.6073503/94‑1 (MQ=60)
                                                                         CTTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATC                      >  SRR4302533.6086055/1‑94 (MQ=60)
                                                                          TTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCT                     <  SRR4302533.2888584/94‑1 (MQ=60)
                                                                          TTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCT                     <  SRR4302533.2656328/94‑1 (MQ=60)
                                                                          TTCCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCT                     >  SRR4302533.2592257/1‑94 (MQ=60)
                                                                            CCGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTC                   <  SRR4302533.5653150/94‑1 (MQ=60)
                                                                             CGCGTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCT                  >  SRR4302533.5723206/1‑94 (MQ=60)
                                                                                GTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATTCAGCGGATCTTCTGAA               >  SRR4302533.5607121/1‑94 (MQ=60)
                                                                                GTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAA               >  SRR4302533.4712835/1‑94 (MQ=60)
                                                                                GTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAA               >  SRR4302533.4971651/1‑94 (MQ=60)
                                                                                GTAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCCTTGGGGGGCTTTCTTTTTATTCAATATCCAACGGATCTTCTGAA               >  SRR4302533.76380/1‑94 (MQ=53)
                                                                                 TAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTTTCATTCAATATCCAGCGGGTCTTTTGAAA              >  SRR4302533.109361/1‑94 (MQ=60)
                                                                                 TAATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAA              >  SRR4302533.1938652/1‑94 (MQ=60)
                                                                                  AATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAG             <  SRR4302533.2187263/94‑1 (MQ=60)
                                                                                  AATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAG             <  SRR4302533.829831/94‑1 (MQ=60)
                                                                                   ATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATTCAGCGGATCTTCTGAAAGA            >  SRR4302533.2657575/1‑94 (MQ=60)
                                                                                   ATTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGA            <  SRR4302533.3089632/94‑1 (MQ=60)
                                                                                    TTTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAA           <  SRR4302533.4104835/94‑1 (MQ=60)
                                                                                     TTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAAT          <  SRR4302533.4069912/94‑1 (MQ=60)
                                                                                     TTCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAAT          <  SRR4302533.1202765/94‑1 (MQ=60)
                                                                                      TCTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAAGCACCTTGCGGGGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATA         >  SRR4302533.4753622/1‑94 (MQ=60)
                                                                                       CTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAA        <  SRR4302533.148102/94‑1 (MQ=60)
                                                                                       CTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAA        >  SRR4302533.1735679/1‑94 (MQ=60)
                                                                                       CTTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAA        <  SRR4302533.234162/94‑1 (MQ=60)
                                                                                        TTTACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAAT       <  SRR4302533.706480/94‑1 (MQ=60)
                                                                                          TACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCC     <  SRR4302533.6174199/94‑1 (MQ=60)
                                                                                          TACCGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCC     <  SRR4302533.5706139/94‑1 (MQ=60)
                                                                                             CGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGG  <  SRR4302533.5464459/94‑1 (MQ=60)
                                                                                             CGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGG  >  SRR4302533.5801112/1‑94 (MQ=60)
                                                                                             |                                                                                             
AACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCCCTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGG  >  NC_000913/4116279‑4116465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑