Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,130,739 A→G T182A (ACT→GCT)  metF → 5,10‑methylenetetrahydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,130,7390AG100.0% 1282.8 / NA 397T182A (ACT→GCT) metF5,10‑methylenetetrahydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (211/186);  total (211/186)

Only 100 of 412 total aligned reads displayed.

TATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATT  >  NC_000913/4130649‑4130828
                                                                                          |                                                                                         
tATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCt                                                                                         >  1:6388262/1‑93 (MQ=255)
tATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCt                                                                                         >  2:943231/1‑93 (MQ=255)
tATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCt                                                                                         >  1:6140326/1‑93 (MQ=255)
tATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCt                                                                                         >  2:3350969/1‑93 (MQ=255)
tATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCt                                                                                         >  1:8590391/1‑93 (MQ=255)
 aTCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTc                                                                                        <  2:6690550/93‑1 (MQ=255)
  tCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCa                                                                                       >  2:4154497/1‑93 (MQ=255)
    cGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGt                                                                                     <  1:6974337/93‑1 (MQ=255)
     ggAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGtt                                                                                    >  2:2860694/1‑93 (MQ=255)
     ggAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGtt                                                                                    >  2:5104186/1‑93 (MQ=255)
      gAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttt                                                                                   >  1:4818536/1‑92 (MQ=255)
       aaGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttct                                                                                  <  2:2292021/93‑1 (MQ=255)
        aGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttctt                                                                                 <  2:5538519/93‑1 (MQ=255)
        aGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttctt                                                                                 <  2:2115560/93‑1 (MQ=255)
        aGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttctt                                                                                 >  2:2474786/1‑93 (MQ=255)
         gTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttt                                                                                >  2:5693133/1‑92 (MQ=255)
         gTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttc                                                                                <  1:8531861/93‑1 (MQ=255)
         gTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttc                                                                                >  1:1072551/1‑93 (MQ=255)
         gTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttc                                                                                >  2:7486676/1‑93 (MQ=255)
          ttCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttct                                                                               <  2:5305223/93‑1 (MQ=255)
           tcgcccGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttctt                                                                              <  1:7960780/90‑1 (MQ=255)
           tCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttctt                                                                              <  1:555347/93‑1 (MQ=255)
           tCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGACAAACGCGCGATTGCTCAGttcttctt                                                                              >  1:5096335/1‑93 (MQ=255)
           tCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGACCCAACCGCGCGATTGCTCAGttcttctt                                                                              <  2:2952182/93‑1 (MQ=255)
              cccGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGa                                                                           <  1:3149164/93‑1 (MQ=255)
              cccGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGa                                                                           <  2:8157545/93‑1 (MQ=255)
              cccGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGa                                                                           <  1:6825689/93‑1 (MQ=255)
               ccGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGAt                                                                          <  2:9230531/93‑1 (MQ=255)
                cGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGCGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATg                                                                         <  2:6371791/93‑1 (MQ=255)
                cGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATg                                                                         <  2:1839481/93‑1 (MQ=255)
                cGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATg                                                                         >  1:1389989/1‑93 (MQ=255)
                 ggAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGt                                                                        <  1:1141495/93‑1 (MQ=255)
                   aaGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCg                                                                      <  2:7820100/93‑1 (MQ=255)
                    aGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGa                                                                     >  2:5172911/1‑93 (MQ=255)
                      cAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGaaa                                                                   <  1:4710179/93‑1 (MQ=255)
                      cAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGaaa                                                                   >  1:570668/1‑93 (MQ=255)
                      cAAAAAGCACTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGaaa                                                                   <  1:6909359/93‑1 (MQ=255)
                       aaaaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAg                                                                  <  1:2209120/93‑1 (MQ=255)
                       aaaaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAg                                                                  >  1:3607804/1‑93 (MQ=255)
                       aaaaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAg                                                                  >  2:6100402/1‑93 (MQ=255)
                       aaaaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAg                                                                  >  1:2699799/1‑93 (MQ=255)
                        aaaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGc                                                                 >  1:6281961/1‑93 (MQ=255)
                        aaaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGc                                                                 <  2:9025735/93‑1 (MQ=255)
                        aaaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGc                                                                 >  1:9097296/1‑93 (MQ=255)
                        aaaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGACAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGc                                                                 >  2:486797/1‑93 (MQ=255)
                         aaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCt                                                                >  2:2008966/1‑93 (MQ=255)
                         aaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCt                                                                >  1:9420094/1‑93 (MQ=255)
                         aaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGACAACCGCGCGATTGCTCAGTTCTTCTTCGCTGTCGAAAGCt                                                                >  1:6682172/1‑93 (MQ=255)
                           aGCGCTCAGTCGGATGTGCTTAATCTGAAACGCAAAGTGGATGTCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTAc                                                              >  2:6954815/1‑93 (MQ=255)
                                tCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCg                                                         >  2:4404505/1‑93 (MQ=255)
                                 cAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGt                                                        >  1:3789925/1‑93 (MQ=255)
                                 cAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGt                                                        >  1:5260255/1‑93 (MQ=255)
                                  aGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGtt                                                       <  2:598989/93‑1 (MQ=255)
                                  aGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGtt                                                       <  2:651145/93‑1 (MQ=255)
                                  aGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGtt                                                       >  1:7412817/1‑93 (MQ=255)
                                    gCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCCACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTAGCTGCGtttt                                                     >  2:5393963/1‑93 (MQ=255)
                                      ggATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCg                                                   >  1:6575602/1‑93 (MQ=255)
                                         tttGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGa                                                >  2:3788018/1‑93 (MQ=255)
                                          ttGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGAc                                               >  1:452676/1‑93 (MQ=255)
                                           tGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGAcc                                              <  1:5811004/93‑1 (MQ=255)
                                            gCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCg                                             >  2:3900699/1‑93 (MQ=255)
                                             cTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGc                                            <  1:9945022/93‑1 (MQ=255)
                                               tAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCtg                                          <  1:3872047/93‑1 (MQ=255)
                                               tAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCtg                                          >  1:9303967/1‑93 (MQ=255)
                                               tAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCtg                                          <  2:1041834/93‑1 (MQ=255)
                                                aaTCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCtgg                                         >  2:7367695/1‑92 (MQ=255)
                                                 aTCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCtgtg                                        >  1:8277802/1‑93 (MQ=255)
                                                  tCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGACAGCTACCTGCGTTTTCGTGACCGCTctgt                                       >  2:3520354/1‑93 (MQ=255)
                                                  tCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTgtgt                                       >  2:8314028/1‑93 (MQ=255)
                                                  tCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTgtgt                                       >  2:8771458/1‑93 (MQ=255)
                                                   cTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTa                                      <  1:1611987/93‑1 (MQ=255)
                                                    tGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTAt                                     <  2:1524537/93‑1 (MQ=255)
                                                     gAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATc                                    >  1:7196558/1‑93 (MQ=255)
                                                     gAAACGCAAAGTGGATGCCGGACCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATc                                    <  2:7169296/93‑1 (MQ=255)
                                                        aCGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATcggc                                 <  2:4561700/93‑1 (MQ=255)
                                                        aCGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATcggc                                 <  1:1209184/93‑1 (MQ=255)
                                                         cGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATcggcg                                <  2:6805038/93‑1 (MQ=255)
                                                          gCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATcggcgg                               <  2:14707/93‑1 (MQ=255)
                                                              aGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCAt                           <  2:4613470/93‑1 (MQ=255)
                                                                tGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCGTTg                         >  1:8913192/1‑93 (MQ=255)
                                                                    tGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCAATGGtgg                     >  1:3474915/1‑92 (MQ=255)
                                                                     gCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAtgtg                    <  1:4101611/93‑1 (MQ=255)
                                                                      ccGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTgg                   <  1:2490252/93‑1 (MQ=255)
                                                                        ggAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGGCTTGATGTggga                 >  2:1679482/1‑91 (MQ=255)
                                                                        ggAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCCTTGATGTGGaa                 >  1:1784910/1‑93 (MQ=255)
                                                                        ggAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGaa                 <  1:3016530/93‑1 (MQ=255)
                                                                        ggAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGaa                 <  1:5463748/93‑1 (MQ=255)
                                                                           gCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGTAatt              <  1:5925438/93‑1 (MQ=255)
                                                                           gCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGAatt              >  1:7889281/1‑93 (MQ=255)
                                                                           gCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGAatt              >  1:9687502/1‑93 (MQ=255)
                                                                              aaCCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGGATTGATTGGGAAattttt           >  1:2003785/1‑93 (MQ=255)
                                                                              aaCCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAattatt           <  2:3079091/93‑1 (MQ=255)
                                                                               aCCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTc          <  2:8515861/93‑1 (MQ=255)
                                                                               aCCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTc          <  1:4298865/93‑1 (MQ=255)
                                                                                ccGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTCATGTGGGAATTATTcc         >  2:2731682/1‑93 (MQ=255)
                                                                                   cgcgATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCggg      <  2:9135088/93‑1 (MQ=255)
                                                                                    gcgATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGGTGTGGGAATTATTCCGGGa     >  1:7664691/1‑93 (MQ=255)
                                                                                     cgATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGaa    <  1:6031949/93‑1 (MQ=255)
                                                                                      gATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTTCGGGAAt   >  1:8427790/1‑93 (MQ=255)
                                                                                       aTTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGGAtt  >  2:3196786/1‑93 (MQ=255)
                                                                                          |                                                                                         
TATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATT  >  NC_000913/4130649‑4130828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 230 total aligned reads displayed.
CGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGC  >  NC_000913/4130647‑4130832
                                                                                            |                                                                                             
CGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGC                                                                                              >  SRR4302540.10150780/1‑94 (MQ=60)
CGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGC                                                                                              <  SRR4302540.4989492/94‑1 (MQ=60)
CGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGC                                                                                              >  SRR4302540.6917505/1‑94 (MQ=60)
 GTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCT                                                                                             >  SRR4302540.6459377/1‑94 (MQ=60)
 GTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCT                                                                                             >  SRR4302540.2068485/1‑94 (MQ=60)
 GTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCT                                                                                             >  SRR4302540.8690254/1‑94 (MQ=60)
 GTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCT                                                                                             >  SRR4302540.8690255/1‑94 (MQ=60)
 GTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCT                                                                                             >  SRR4302540.6208712/1‑94 (MQ=60)
  TATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTC                                                                                            <  SRR4302540.16854/94‑1 (MQ=60)
   ATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCA                                                                                           >  SRR4302540.4171924/1‑94 (MQ=60)
   ATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGC‑TAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGCTTGCTCAG                                                                                          >  SRR4302540.68876/1‑94 (MQ=60)
     CCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGT                                                                                         >  SRR4302540.7225763/1‑94 (MQ=60)
     CCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGT                                                                                         >  SRR4302540.8777739/1‑94 (MQ=60)
      CGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTT                                                                                        <  SRR4302540.7051842/94‑1 (MQ=60)
      CGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTT                                                                                        >  SRR4302540.6033210/1‑94 (MQ=60)
       GGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTT                                                                                       >  SRR4302540.4870114/1‑94 (MQ=60)
       GGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTC                                                                                       >  SRR4302540.7492189/1‑94 (MQ=60)
       GGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTC                                                                                       >  SRR4302540.1670090/1‑94 (MQ=60)
          AGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTC                                                                                    >  SRR4302540.9789416/1‑94 (MQ=60)
          AGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTC                                                                                    >  SRR4302540.1083497/1‑94 (MQ=60)
          AGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTC                                                                                    >  SRR4302540.1083498/1‑94 (MQ=60)
          AGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTC                                                                                    >  SRR4302540.8943102/1‑94 (MQ=60)
          AGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTC                                                                                    <  SRR4302540.4935811/94‑1 (MQ=60)
           GTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCT                                                                                   <  SRR4302540.8630990/94‑1 (MQ=60)
           GTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCT                                                                                   <  SRR4302540.3557711/94‑1 (MQ=60)
           GTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCT                                                                                   >  SRR4302540.6844645/1‑94 (MQ=60)
            TTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTT                                                                                  >  SRR4302540.5151480/1‑94 (MQ=60)
            TTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGACAAACGCGCGATTGCTCAGTTCTTCTT                                                                                  >  SRR4302540.5151481/1‑94 (MQ=60)
             TCACCCGGAAGCAAAAAGCGTTCAGGCGGATTTGCTTAGTCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTC                                                                                 <  SRR4302540.561405/94‑1 (MQ=60)
             TCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTC                                                                                 <  SRR4302540.561406/94‑1 (MQ=60)
             TCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTC                                                                                 <  SRR4302540.5670119/94‑1 (MQ=60)
               ACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGA                                                                               >  SRR4302540.315158/1‑94 (MQ=60)
               ACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGA                                                                               >  SRR4302540.5692387/1‑94 (MQ=60)
                 CCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATT                                                                             >  SRR4302540.8034642/1‑94 (MQ=60)
                 CCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATG                                                                             >  SRR4302540.1404867/1‑94 (MQ=60)
                 CCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATG                                                                             >  SRR4302540.8034643/1‑94 (MQ=60)
                 CCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATG                                                                             >  SRR4302540.4508832/1‑94 (MQ=60)
                 CCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATG                                                                             <  SRR4302540.1875369/94‑1 (MQ=60)
                 CCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATG                                                                             >  SRR4302540.5835034/1‑94 (MQ=60)
                   GGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTC                                                                           <  SRR4302540.267957/94‑1 (MQ=60)
                   GGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTC                                                                           <  SRR4302540.7017168/94‑1 (MQ=60)
                   GGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTC                                                                           <  SRR4302540.5672525/94‑1 (MQ=60)
                   GGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTC                                                                           <  SRR4302540.8111513/94‑1 (MQ=60)
                   CGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTC                                                                           <  SRR4302540.7184927/94‑1 (MQ=60)
                    GAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCG                                                                          >  SRR4302540.2128819/1‑94 (MQ=60)
                     AAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGA                                                                         >  SRR4302540.5513324/1‑94 (MQ=60)
                     AAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGA                                                                         >  SRR4302540.6728557/1‑94 (MQ=60)
                      AGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAA                                                                        >  SRR4302540.9222083/1‑94 (MQ=60)
                      AGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAA                                                                        >  SRR4302540.1157142/1‑94 (MQ=60)
                      AGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAA                                                                        >  SRR4302540.4466138/1‑94 (MQ=60)
                       GCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAA                                                                       >  SRR4302540.3010536/1‑94 (MQ=60)
                       GCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAA                                                                       >  SRR4302540.576866/1‑94 (MQ=60)
                       GCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAA                                                                       <  SRR4302540.8388305/94‑1 (MQ=60)
                        CAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAG                                                                      <  SRR4302540.4760518/94‑1 (MQ=60)
                        CAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAG                                                                      >  SRR4302540.6234165/1‑94 (MQ=60)
                        CAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAG                                                                      >  SRR4302540.9738672/1‑94 (MQ=60)
                        CAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAG                                                                      >  SRR4302540.2728937/1‑94 (MQ=60)
                        CAAAAAGCACTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAG                                                                      <  SRR4302540.6986221/94‑1 (MQ=60)
                         AAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGC                                                                     >  SRR4302540.6351871/1‑94 (MQ=60)
                         AAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGC                                                                     <  SRR4302540.5991647/94‑1 (MQ=60)
                         AAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGC                                                                     <  SRR4302540.2232983/94‑1 (MQ=60)
                         AAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGC                                                                     >  SRR4302540.2155832/1‑94 (MQ=60)
                         AAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGC                                                                     >  SRR4302540.9206602/1‑94 (MQ=60)
                         AAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGC                                                                     <  SRR4302540.9056266/94‑1 (MQ=60)
                          AAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCT                                                                    >  SRR4302540.9532062/1‑94 (MQ=60)
                          AAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCT                                                                    >  SRR4302540.1174759/1‑94 (MQ=60)
                          AAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCT                                                                    >  SRR4302540.1442340/1‑94 (MQ=60)
                          AAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGACAACCGCGCGATTGCTCAGTTCTTCTTCGCTGTCGAAAGCT                                                                    >  SRR4302540.6755981/1‑94 (MQ=60)
                           AAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGACAGCTA                                                                   >  SRR4302540.4826783/1‑94 (MQ=60)
                           AAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTA                                                                   >  SRR4302540.3798720/1‑94 (MQ=60)
                           AAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTA                                                                   >  SRR4302540.750506/1‑94 (MQ=60)
                             AGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACC                                                                 <  SRR4302540.8639834/94‑1 (MQ=60)
                             AGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACC                                                                 >  SRR4302540.8141611/1‑94 (MQ=60)
                             AGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACC                                                                 >  SRR4302540.191247/1‑94 (MQ=60)
                             AGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACC                                                                 >  SRR4302540.2233307/1‑94 (MQ=60)
                             AGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACC                                                                 <  SRR4302540.6934898/94‑1 (MQ=60)
                               CGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTG                                                               <  SRR4302540.3518061/94‑1 (MQ=60)
                                GCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGC                                                              <  SRR4302540.987427/94‑1 (MQ=60)
                                 CTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCG                                                             >  SRR4302540.8037611/1‑94 (MQ=60)
                                 CTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCG                                                             >  SRR4302540.760880/1‑94 (MQ=60)
                                  TCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGGTTGCTCAGTTCTTCTTCGATGTCGAAAGCTAACTGCGT                                                            >  SRR4302540.8577785/1‑94 (MQ=60)
                                  TCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGT                                                            >  SRR4302540.3831245/1‑94 (MQ=60)
                                  TCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGT                                                            >  SRR4302540.5317647/1‑94 (MQ=60)
                                   CAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTT                                                           >  SRR4302540.2295081/1‑94 (MQ=60)
                                   CAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTT                                                           >  SRR4302540.6522629/1‑94 (MQ=60)
                                   CAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTT                                                           >  SRR4302540.309787/1‑94 (MQ=60)
                                   CAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTT                                                           >  SRR4302540.7495382/1‑94 (MQ=60)
                                    AGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTT                                                          >  SRR4302540.6827481/1‑94 (MQ=60)
                                    AGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTT                                                          <  SRR4302540.7139285/94‑1 (MQ=60)
                                      GCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTC                                                        <  SRR4302540.5855604/94‑1 (MQ=60)
                                       CGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCG                                                       >  SRR4302540.6648440/1‑94 (MQ=60)
                                        GGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGT                                                      <  SRR4302540.6386966/94‑1 (MQ=60)
                                        GGATTTGCTTAATCTGAAACGCAAAGTGGATGACGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGT                                                      <  SRR4302540.3738349/94‑1 (MQ=60)
                                        CGATTTTCTTAATCTGAAACGCAAAGTGGATGACGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGT                                                      <  SRR4302540.3738350/94‑1 (MQ=60)
                                         GATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTG                                                     <  SRR4302540.1159372/94‑1 (MQ=60)
                                         GATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTG                                                     <  SRR4302540.255602/94‑1 (MQ=60)
                                          ATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGA                                                    >  SRR4302540.6070334/1‑94 (MQ=60)
                                          ATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGA                                                    >  SRR4302540.3865409/1‑94 (MQ=60)
                                          ATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGA                                                    >  SRR4302540.6915848/1‑94 (MQ=60)
                                           TTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGAC                                                   >  SRR4302540.457507/1‑94 (MQ=60)
                                            TTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACC                                                  <  SRR4302540.4740953/94‑1 (MQ=60)
                                             TGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCG                                                 <  SRR4302540.5875229/94‑1 (MQ=60)
                                               CTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCT                                               <  SRR4302540.10061155/94‑1 (MQ=60)
                                               CTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCT                                               >  SRR4302540.9464158/1‑94 (MQ=60)
                                               CTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCT                                               >  SRR4302540.167198/1‑94 (MQ=60)
                                               CTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCT                                               >  SRR4302540.5365236/1‑94 (MQ=60)
                                                TTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTG                                              >  SRR4302540.9415095/1‑94 (MQ=60)
                                                TTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTG                                              >  SRR4302540.9415094/1‑94 (MQ=60)
                                                 TAATCTGAAACGCAAAGTGGATGCCGGGGCCAACCGCGCGATTGCTCAGTTATTTTTCGATGCGGACAGCTACCTGCGTTTTGGTTCCCGCTGT                                             >  SRR4302540.9432636/1‑94 (MQ=48)
                                                 TAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGT                                             <  SRR4302540.3914140/94‑1 (MQ=60)
                                                 TAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGT                                             >  SRR4302540.7036069/1‑94 (MQ=60)
                                                 TAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGT                                             >  SRR4302540.9432635/1‑94 (MQ=60)
                                                  AATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTG                                            >  SRR4302540.8373953/1‑94 (MQ=60)
                                                   ATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGT                                           >  SRR4302540.4190127/1‑94 (MQ=60)
                                                   ATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGT                                           >  SRR4302540.3939045/1‑94 (MQ=60)
                                                   ATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGT                                           >  SRR4302540.2712846/1‑94 (MQ=60)
                                                   ATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGT                                           <  SRR4302540.7465763/94‑1 (MQ=60)
                                                    TCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTT                                          >  SRR4302540.6517114/1‑94 (MQ=60)
                                                    TCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCCGTGTT                                          >  SRR4302540.1228432/1‑94 (MQ=60)
                                                      TGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATC                                        >  SRR4302540.7276776/1‑94 (MQ=60)
                                                      TGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATC                                        >  SRR4302540.4918541/1‑94 (MQ=60)
                                                      TGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATC                                        >  SRR4302540.6659818/1‑94 (MQ=60)
                                                       GAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCG                                       >  SRR4302540.6951498/1‑94 (MQ=60)
                                                       GAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCG                                       <  SRR4302540.9712431/94‑1 (MQ=60)
                                                        AAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGG                                      <  SRR4302540.3890341/94‑1 (MQ=60)
                                                          ACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCG                                    <  SRR4302540.1449361/94‑1 (MQ=60)
                                                          ACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCG                                    <  SRR4302540.1221589/94‑1 (MQ=60)
                                                           CGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGG                                   >  SRR4302540.3445989/1‑94 (MQ=60)
                                                           CGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGG                                   >  SRR4302540.3979021/1‑94 (MQ=60)
                                                           CGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGG                                   <  SRR4302540.5599484/94‑1 (MQ=60)
                                                            GCAAAGTCGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGG                                  <  SRR4302540.4853862/94‑1 (MQ=60)
                                                              AAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCT                                >  SRR4302540.926647/1‑94 (MQ=60)
                                                              AAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCG                                >  SRR4302540.5316334/1‑94 (MQ=60)
                                                              AAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCA                                >  SRR4302540.8500986/1‑94 (MQ=60)
                                                              AAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCA                                >  SRR4302540.5316332/1‑94 (MQ=60)
                                                              AAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCATCGATGTCGAAAGCTACCTGCGTTTTCGTGAACGCTGTGTATCGGCGGGCA                                >  SRR4302540.4325559/1‑94 (MQ=60)
                                                                AGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCGTT                              >  SRR4302540.4723285/1‑94 (MQ=60)
                                                                AGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATT                              <  SRR4302540.299135/94‑1 (MQ=60)
                                                                 GTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTG                             <  SRR4302540.7958353/94‑1 (MQ=60)
                                                                  TGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGggtttg                            >  SRR4302540.8264646/1‑88 (MQ=60)
                                                                  TGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGA                            <  SRR4302540.8613295/94‑1 (MQ=60)
                                                                  TGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGA                            >  SRR4302540.8264645/1‑94 (MQ=60)
                                                                    GATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCAgtgcag                          >  SRR4302540.9087248/1‑88 (MQ=60)
                                                                     GTGCCGGCGCCAACCGCGCGATTGCTCAGCTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGT                         <  SRR4302540.852146/94‑1 (MQ=60)
                                                                     ATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCcttcttgg                         >  SRR4302540.8729945/1‑86 (MQ=60)
                                                                     ATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCAatggtgg                         >  SRR4302540.3512753/1‑87 (MQ=60)
                                                                     ATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGT                         <  SRR4302540.2653592/94‑1 (MQ=60)
                                                                     ATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGT                         <  SRR4302540.6098961/94‑1 (MQ=60)
                                                                     ATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGG                         >  SRR4302540.5804694/1‑94 (MQ=60)
                                                                      TGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG                        <  SRR4302540.6163865/94‑1 (MQ=60)
                                                                       GCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG                       <  SRR4302540.4145860/94‑1 (MQ=60)
                                                                       GCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG                       <  SRR4302540.6758086/94‑1 (MQ=60)
                                                                       GCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG                       <  SRR4302540.5985660/94‑1 (MQ=60)
                                                                       GCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG                       <  SRR4302540.3536336/94‑1 (MQ=60)
                                                                       GCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG                       <  SRR4302540.9114159/94‑1 (MQ=60)
                                                                         CGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCCTTGATGTGGAA                     >  SRR4302540.1804297/1‑94 (MQ=60)
                                                                         CGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGA                     >  SRR4302540.6236531/1‑94 (MQ=60)
                                                                         CGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAA                     >  SRR4302540.6385610/1‑94 (MQ=60)
                                                                          GGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAA                    <  SRR4302540.5523887/94‑1 (MQ=60)
                                                                          GGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAA                    >  SRR4302540.3218651/1‑94 (MQ=60)
                                                                          GGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAA                    <  SRR4302540.3049689/94‑1 (MQ=60)
                                                                            AGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGGACTGATGTTGAAATT                  >  SRR4302540.1087811/1‑94 (MQ=60)
                                                                            AGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGAATT                  >  SRR4302540.9801459/1‑94 (MQ=60)
                                                                            AGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGAATT                  >  SRR4302540.7981364/1‑94 (MQ=60)
                                                                            AGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATT                  >  SRR4302540.5896354/1‑94 (MQ=60)
                                                                             GCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGTAATTA                 <  SRR4302540.5990907/94‑1 (MQ=60)
                                                                              CCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTAT                >  SRR4302540.3140271/1‑94 (MQ=60)
                                                                              CCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTAT                <  SRR4302540.7950878/94‑1 (MQ=60)
                                                                               CAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCGTTGATGTGGGAATTATT               >  SRR4302540.1922556/1‑94 (MQ=60)
                                                                               CAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATT               <  SRR4302540.224484/94‑1 (MQ=60)
                                                                                 ACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGTTCTGATGTGGAAATTATTCC             >  SRR4302540.5408749/1‑94 (MQ=60)
                                                                                 ACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTTC             >  SRR4302540.9809934/1‑94 (MQ=60)
                                                                                 ACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCC             <  SRR4302540.4358703/94‑1 (MQ=60)
                                                                                 ACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCC             <  SRR4302540.4345654/94‑1 (MQ=60)
                                                                                  CCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCG            <  SRR4302540.1350911/94‑1 (MQ=60)
                                                                                  CCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCG            <  SRR4302540.1299955/94‑1 (MQ=60)
                                                                                   CGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTTATGGGGGAATTATTTCGG           >  SRR4302540.8165616/1‑94 (MQ=60)
                                                                                   CGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGAATTATTTCGG           >  SRR4302540.645749/1‑94 (MQ=60)
                                                                                   CGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTtctg           >  SRR4302540.6343487/1‑90 (MQ=60)
                                                                                   CGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGG           >  SRR4302540.1090143/1‑94 (MQ=60)
                                                                                    GCGCTATTGCTCAGTTCTTCTTCGATGGCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGG          <  SRR4302540.7577077/94‑1 (MQ=60)
                                                                                    GCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGG          <  SRR4302540.5936677/94‑1 (MQ=60)
                                                                                     CGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGGTGTGGGAATTATTCCGGGA         >  SRR4302540.7751031/1‑94 (MQ=60)
                                                                                     CGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGA         <  SRR4302540.881288/94‑1 (MQ=60)
                                                                                      GCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTTCGGGAA        >  SRR4302540.473297/1‑94 (MQ=60)
                                                                                       CGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTTCGGGAAT       >  SRR4302540.8525631/1‑94 (MQ=60)
                                                                                       CGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAAT       <  SRR4302540.148371/94‑1 (MQ=60)
                                                                                       CGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAAT       <  SRR4302540.6098894/94‑1 (MQ=60)
                                                                                       CGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAAT       >  SRR4302540.3494791/1‑94 (MQ=60)
                                                                                        GATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATT      <  SRR4302540.3958739/94‑1 (MQ=60)
                                                                                         ATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCCTTGATGTGGAAATTATTCCGGGAATTT     >  SRR4302540.6973389/1‑94 (MQ=60)
                                                                                          TTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCCTTGGTGTGGAAATTATTCCGGGAATTTT    >  SRR4302540.5169376/1‑94 (MQ=60)
                                                                                          TTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGAATTATTCCGGGAATTTT    >  SRR4302540.291717/1‑94 (MQ=60)
                                                                                          TTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGGATTTT    >  SRR4302540.9741460/1‑94 (MQ=60)
                                                                                          TTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTT    >  SRR4302540.1343826/1‑94 (MQ=60)
                                                                                          TTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTT    <  SRR4302540.4584411/94‑1 (MQ=60)
                                                                                           TGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGAATTATTCCGGGGATTTTG   >  SRR4302540.2583703/1‑94 (MQ=60)
                                                                                            GCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCCTTGATGTGGGAAATATTCCGGGAATTTTtg  >  SRR4302540.9273991/1‑92 (MQ=60)
                                                                                            GCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGC  <  SRR4302540.1210682/94‑1 (MQ=60)
                                                                                            GCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGC  >  SRR4302540.10082953/1‑94 (MQ=60)
                                                                                            |                                                                                             
CGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGC  >  NC_000913/4130647‑4130832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑