Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,130,739 A→G T182A (ACT→GCT)  metF → 5,10‑methylenetetrahydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,130,7390AG100.0% 704.1 / NA 218T182A (ACT→GCT) metF5,10‑methylenetetrahydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (101/117);  total (101/117)

Only 100 of 218 total aligned reads displayed.

TATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAAT  >  NC_000913/4130649‑4130827
                                                                                          |                                                                                        
tATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCt                                                                                        >  1:4147628/1‑93 (MQ=255)
tATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCt                                                                                        <  2:1335113/93‑1 (MQ=255)
 aTCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTc                                                                                       >  1:171649/1‑93 (MQ=255)
  tCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCa                                                                                      <  1:1156072/93‑1 (MQ=255)
  tCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCa                                                                                      <  1:1549855/93‑1 (MQ=255)
  tCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCa                                                                                      <  2:6105370/93‑1 (MQ=255)
  tCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCa                                                                                      >  2:4862250/1‑93 (MQ=255)
   ccGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAg                                                                                     <  1:5345039/93‑1 (MQ=255)
     ggAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGtt                                                                                   >  2:2322539/1‑93 (MQ=255)
      gAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttc                                                                                  <  1:2085684/93‑1 (MQ=255)
       aaGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttct                                                                                 >  1:1431357/1‑93 (MQ=255)
       aaGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttct                                                                                 <  2:686271/93‑1 (MQ=255)
        aGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttctt                                                                                >  2:1231242/1‑93 (MQ=255)
         gTTCACCCGGAAGGAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttc                                                                               <  2:1133622/93‑1 (MQ=255)
         gTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttc                                                                               <  1:4194445/93‑1 (MQ=255)
          ttCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttct                                                                              <  1:4295656/93‑1 (MQ=255)
          ttCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttct                                                                              >  2:3700100/1‑93 (MQ=255)
           tCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttctt                                                                             <  2:6033781/93‑1 (MQ=255)
            cACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttcttc                                                                            >  1:493193/1‑93 (MQ=255)
             aCCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAACTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCg                                                                           <  1:3379126/93‑1 (MQ=255)
               ccGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGAt                                                                         <  1:624506/93‑1 (MQ=255)
               ccGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGAt                                                                         <  2:5965393/93‑1 (MQ=255)
               ccGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGAt                                                                         <  2:2136373/93‑1 (MQ=255)
                cGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATg                                                                        >  2:246849/1‑93 (MQ=255)
                 ggAAGCAGAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGt                                                                       >  1:3118140/1‑93 (MQ=255)
                 ggAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGt                                                                       <  2:2009326/93‑1 (MQ=255)
                 ggAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGt                                                                       <  2:1304237/93‑1 (MQ=255)
                  gAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTc                                                                      <  2:734357/93‑1 (MQ=255)
                   aaGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCg                                                                     <  1:296816/93‑1 (MQ=255)
                    aGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGa                                                                    <  1:4049850/93‑1 (MQ=255)
                     gCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGaa                                                                   >  1:3296317/1‑93 (MQ=255)
                       aaaaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAg                                                                 >  1:641855/1‑93 (MQ=255)
                            gcgcTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTAcc                                                            >  2:434779/1‑93 (MQ=255)
                            gcgcTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTAcc                                                            >  1:5178948/1‑93 (MQ=255)
                            gcgcTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTAcc                                                            <  2:411052/93‑1 (MQ=255)
                             cgcTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCt                                                           <  1:5996308/93‑1 (MQ=255)
                                tCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCg                                                        >  1:2330494/1‑93 (MQ=255)
                                  aGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGtt                                                      <  2:3158862/93‑1 (MQ=255)
                                  aGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGtt                                                      >  2:4157486/1‑93 (MQ=255)
                                   ggCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGttt                                                     >  2:3972652/1‑93 (MQ=255)
                                    gCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGtttt                                                    <  2:1942035/93‑1 (MQ=255)
                                     cGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTc                                                   >  1:4571936/1‑93 (MQ=255)
                                     cGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTc                                                   <  2:798154/93‑1 (MQ=255)
                                       gATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGt                                                 >  1:3818215/1‑93 (MQ=255)
                                       gATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGt                                                 >  2:3253376/1‑93 (MQ=255)
                                       gATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGt                                                 <  1:1919568/93‑1 (MQ=255)
                                       gATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGt                                                 >  2:4240138/1‑93 (MQ=255)
                                        aTTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTg                                                >  2:1649030/1‑93 (MQ=255)
                                         tttGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGa                                               >  2:1108289/1‑93 (MQ=255)
                                          ttGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGAc                                              >  2:5476025/1‑93 (MQ=255)
                                          ttGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGAc                                              <  1:5377734/93‑1 (MQ=255)
                                           tGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGAcc                                             >  2:2825755/1‑93 (MQ=255)
                                             cTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGc                                           <  1:5293590/93‑1 (MQ=255)
                                             cTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGc                                           >  1:3989434/1‑93 (MQ=255)
                                               tAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCtg                                         <  1:2594036/93‑1 (MQ=255)
                                                  tCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTgtgt                                      <  2:5101592/93‑1 (MQ=255)
                                                  tCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTgtgt                                      >  1:2784181/1‑93 (MQ=255)
                                                     gAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATc                                   <  1:816769/93‑1 (MQ=255)
                                                     gAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATc                                   <  1:5596733/93‑1 (MQ=255)
                                                     gAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATc                                   <  1:180752/93‑1 (MQ=255)
                                                     gAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATc                                   <  2:4567481/93‑1 (MQ=255)
                                                       aaCGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATcgg                                 <  1:4434866/93‑1 (MQ=255)
                                                        aCGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATcggc                                <  2:5172958/93‑1 (MQ=255)
                                                          gCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATcggcgg                              >  2:5905860/1‑93 (MQ=255)
                                                          gCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATcggcgg                              >  1:3593837/1‑93 (MQ=255)
                                                            aaaGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGc                            <  1:1696112/93‑1 (MQ=255)
                                                            aaaGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGc                            <  1:185040/93‑1 (MQ=255)
                                                                 ggATGCCGGATCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGa                       <  1:1003924/93‑1 (MQ=255)
                                                                 ggATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGa                       >  1:3442565/1‑93 (MQ=255)
                                                                 ggATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGa                       <  2:4777466/93‑1 (MQ=255)
                                                                 ggATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGa                       <  1:3161578/93‑1 (MQ=255)
                                                                     gCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAtgtg                   >  2:3364566/1‑93 (MQ=255)
                                                                      ccGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTgg                  <  2:256028/93‑1 (MQ=255)
                                                                      ccGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTgg                  <  1:5904005/93‑1 (MQ=255)
                                                                       cGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGGATTTATGTggg                 >  2:3477/1‑92 (MQ=255)
                                                                        ggAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTTATGTggga                >  1:333686/1‑91 (MQ=255)
                                                                        ggAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGaa                >  1:5023758/1‑93 (MQ=255)
                                                                         gAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGGATTGATGTGGgaa               >  2:2640659/1‑93 (MQ=255)
                                                                         gAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGaaa               <  2:2612505/93‑1 (MQ=255)
                                                                         gAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGaaa               <  2:5555958/93‑1 (MQ=255)
                                                                          aGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGAat              >  2:3323002/1‑93 (MQ=255)
                                                                            ccAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGAatta            >  2:4815259/1‑93 (MQ=255)
                                                                            ccAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAatta            <  1:1123434/93‑1 (MQ=255)
                                                                             cAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGAattat           >  2:2853894/1‑93 (MQ=255)
                                                                               aCCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTc         <  2:3022882/93‑1 (MQ=255)
                                                                               aCCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTc         >  2:833407/1‑93 (MQ=255)
                                                                               aCCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTc         <  1:4709915/93‑1 (MQ=255)
                                                                                ccGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTcc        <  1:621114/93‑1 (MQ=255)
                                                                                ccGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTcc        <  1:5060655/93‑1 (MQ=255)
                                                                                 cgcgcgATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCg       <  1:1305277/93‑1 (MQ=255)
                                                                                  gcgcgATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTTCgg      >  1:3695595/1‑93 (MQ=255)
                                                                                   cgcgATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCggg     <  1:903990/93‑1 (MQ=255)
                                                                                   cgcgATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCggg     <  2:2960449/93‑1 (MQ=255)
                                                                                    gcgATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGGATTTATGTGGAAATTATTCCGGGa    >  1:89399/1‑93 (MQ=255)
                                                                                    gcgATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCGTTGATGTGGGAATTATTCCCGGa    >  2:3619926/1‑93 (MQ=255)
                                                                                    gcgATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGa    <  1:3622424/93‑1 (MQ=255)
                                                                                    gcgATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGa    <  1:2205013/93‑1 (MQ=255)
                                                                                     cgATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGaa   <  2:5731173/93‑1 (MQ=255)
                                                                                      gATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCCTTGATGTGGAAATTATTCCGGGCAt  >  1:1170250/1‑93 (MQ=255)
                                                                                      gATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAAt  <  1:491272/93‑1 (MQ=255)
                                                                                          |                                                                                        
TATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAAT  >  NC_000913/4130649‑4130827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGC  >  NC_000913/4130646‑4130832
                                                                                             |                                                                                             
GCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTG                                                                                               <  SRR4302533.2245756/94‑1 (MQ=60)
GCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTG                                                                                               >  SRR4302533.1926618/1‑94 (MQ=60)
GCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTG                                                                                               >  SRR4302533.1320911/1‑94 (MQ=60)
 CGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGC                                                                                              >  SRR4302533.2946498/1‑94 (MQ=60)
  GTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCATCCGCGCGATTGCT                                                                                             >  SRR4302533.3626717/1‑94 (MQ=60)
  GTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCT                                                                                             <  SRR4302533.4107297/94‑1 (MQ=60)
  GTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCT                                                                                             >  SRR4302533.4172756/1‑94 (MQ=60)
   TATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTC                                                                                            >  SRR4302533.172731/1‑94 (MQ=60)
     TCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAG                                                                                          <  SRR4302533.1163004/94‑1 (MQ=60)
     TCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAG                                                                                          <  SRR4302533.1559194/94‑1 (MQ=60)
      CCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGT                                                                                         <  SRR4302533.5377093/94‑1 (MQ=60)
       CGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTT                                                                                        <  SRR4302533.1556864/94‑1 (MQ=60)
        GGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTC                                                                                       <  SRR4302533.4945961/94‑1 (MQ=60)
         GAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCT                                                                                      <  SRR4302533.2098528/94‑1 (MQ=60)
         GAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCT                                                                                      >  SRR4302533.1440007/1‑94 (MQ=60)
           AGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGACAACCGCGCGATTGCTCAGTTCTTC                                                                                    >  SRR4302533.1312220/1‑94 (MQ=60)
            GTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCT                                                                                   <  SRR4302533.4219833/94‑1 (MQ=60)
            GTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCT                                                                                   <  SRR4302533.2989338/94‑1 (MQ=60)
            GTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCT                                                                                   <  SRR4302533.891119/94‑1 (MQ=60)
             TTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTT                                                                                  <  SRR4302533.4321491/94‑1 (MQ=60)
              TCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTC                                                                                 >  SRR4302533.496137/1‑94 (MQ=60)
                ACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGA                                                                               >  SRR4302533.1955175/1‑94 (MQ=60)
                ACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAACTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGA                                                                               <  SRR4302533.3399986/94‑1 (MQ=60)
                  CCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATG                                                                             <  SRR4302533.628172/94‑1 (MQ=60)
                   CGGAAGCAGAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGT                                                                            >  SRR4302533.3137313/1‑94 (MQ=60)
                    GGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTC                                                                           <  SRR4302533.1773987/94‑1 (MQ=60)
                     GAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCG                                                                          <  SRR4302533.3443714/94‑1 (MQ=60)
                      AAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGA                                                                         <  SRR4302533.298634/94‑1 (MQ=60)
                      AAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGA                                                                         <  SRR4302533.5297883/94‑1 (MQ=60)
                       AGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAA                                                                        >  SRR4302533.3316663/1‑94 (MQ=60)
                       AGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAA                                                                        >  SRR4302533.4775084/1‑94 (MQ=60)
                       AGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAA                                                                        <  SRR4302533.4074519/94‑1 (MQ=60)
                        GCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAA                                                                       <  SRR4302533.4238925/94‑1 (MQ=60)
                         CAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAG                                                                      >  SRR4302533.645629/1‑94 (MQ=60)
                          AAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGC                                                                     <  SRR4302533.2104420/94‑1 (MQ=60)
                          AAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGC                                                                     >  SRR4302533.6317025/1‑94 (MQ=60)
                           AAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCT                                                                    >  SRR4302533.3696535/1‑94 (MQ=60)
                              AGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACC                                                                 >  SRR4302533.5210225/1‑94 (MQ=60)
                                CGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTG                                                               <  SRR4302533.6032624/94‑1 (MQ=60)
                                CGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTG                                                               <  SRR4302533.759997/94‑1 (MQ=60)
                                 GCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGC                                                              <  SRR4302533.4625502/94‑1 (MQ=60)
                                  CTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCG                                                             >  SRR4302533.2344924/1‑94 (MQ=60)
                                     AGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTT                                                          >  SRR4302533.5629811/1‑94 (MQ=60)
                                     AGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTT                                                          >  SRR4302533.4512617/1‑94 (MQ=60)
                                      GGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTT                                                         >  SRR4302533.1139236/1‑94 (MQ=60)
                                       GCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTC                                                        <  SRR4302533.4427566/94‑1 (MQ=60)
                                       GCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTC                                                        <  SRR4302533.3525880/94‑1 (MQ=60)
                                       GCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTC                                                        >  SRR4302533.4599641/1‑94 (MQ=60)
                                        CGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCG                                                       >  SRR4302533.907689/1‑94 (MQ=60)
                                        CGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCG                                                       >  SRR4302533.4553786/1‑94 (MQ=60)
                                         GGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGT                                                      >  SRR4302533.3841562/1‑94 (MQ=60)
                                         GGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGT                                                      <  SRR4302533.4047307/94‑1 (MQ=60)
                                          GATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTG                                                     <  SRR4302533.1931286/94‑1 (MQ=60)
                                           ATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGA                                                    >  SRR4302533.5028983/1‑94 (MQ=60)
                                           ATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGACAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGA                                                    >  SRR4302533.4087161/1‑94 (MQ=60)
                                             TTTCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACC                                                  <  SRR4302533.3317067/94‑1 (MQ=60)
                                             TTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACC                                                  <  SRR4302533.6081656/94‑1 (MQ=60)
                                             TTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACC                                                  <  SRR4302533.4213588/94‑1 (MQ=60)
                                             TTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACC                                                  <  SRR4302533.5410050/94‑1 (MQ=60)
                                             TTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACC                                                  >  SRR4302533.5381159/1‑94 (MQ=60)
                                               GCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGC                                                >  SRR4302533.953065/1‑94 (MQ=60)
                                               GCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGC                                                >  SRR4302533.3958376/1‑94 (MQ=60)
                                               GCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGC                                                >  SRR4302533.4013733/1‑94 (MQ=60)
                                                CTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCT                                               <  SRR4302533.5325278/94‑1 (MQ=60)
                                                CTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCT                                               >  SRR4302533.1861949/1‑94 (MQ=60)
                                                  TAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGT                                             <  SRR4302533.3187296/94‑1 (MQ=60)
                                                  TAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGT                                             <  SRR4302533.2609974/94‑1 (MQ=60)
                                                    ATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGT                                           >  SRR4302533.2801288/1‑94 (MQ=60)
                                                     TCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTA                                          <  SRR4302533.296785/94‑1 (MQ=60)
                                                     TCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTA                                          >  SRR4302533.2525845/1‑94 (MQ=60)
                                                     TCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTA                                          >  SRR4302533.1654496/1‑94 (MQ=60)
                                                       TGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATC                                        <  SRR4302533.5079614/94‑1 (MQ=60)
                                                       TGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATC                                        <  SRR4302533.1023991/94‑1 (MQ=60)
                                                       TGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATC                                        >  SRR4302533.171426/1‑94 (MQ=60)
                                                        GAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCG                                       <  SRR4302533.181878/94‑1 (MQ=60)
                                                        GAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCG                                       <  SRR4302533.3163475/94‑1 (MQ=60)
                                                        GAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCG                                       <  SRR4302533.821555/94‑1 (MQ=60)
                                                        GAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCG                                       <  SRR4302533.5630549/94‑1 (MQ=60)
                                                         AAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGG                                      <  SRR4302533.1169207/94‑1 (MQ=60)
                                                          AACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGC                                     <  SRR4302533.4461675/94‑1 (MQ=60)
                                                           ACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCG                                    >  SRR4302533.1526719/1‑94 (MQ=60)
                                                            CGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGG                                   >  SRR4302533.3615950/1‑94 (MQ=60)
                                                              CAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGC                                 >  SRR4302533.3365286/1‑94 (MQ=60)
                                                              CAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGC                                 >  SRR4302533.1423207/1‑94 (MQ=60)
                                                               AAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCA                                <  SRR4302533.186189/94‑1 (MQ=60)
                                                               AAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCA                                >  SRR4302533.1852507/1‑94 (MQ=60)
                                                               AAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCA                                <  SRR4302533.1706384/94‑1 (MQ=60)
                                                                  GTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTG                             <  SRR4302533.2239561/94‑1 (MQ=60)
                                                                   TGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGA                            >  SRR4302533.3463828/1‑94 (MQ=60)
                                                                    GGATGCCGGATCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT                           <  SRR4302533.1009793/94‑1 (MQ=60)
                                                                    GGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT                           <  SRR4302533.3181034/94‑1 (MQ=60)
                                                                         CCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGA                      <  SRR4302533.5939661/94‑1 (MQ=60)
                                                                          CGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGGATTGATGTGGGA                     >  SRR4302533.193418/1‑94 (MQ=60)
                                                                          CGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTTATGTGGGA                     >  SRR4302533.335726/1‑94 (MQ=60)
                                                                          CGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAA                     >  SRR4302533.5054181/1‑94 (MQ=60)
                                                                          CGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAA                     >  SRR4302533.5682536/1‑94 (MQ=60)
                                                                            GAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAAT                   <  SRR4302533.2834219/94‑1 (MQ=60)
                                                                             ATCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAAAT                  >  SRR4302533.5977574/1‑94 (MQ=60)
                                                                              GCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCACTGATGTGGGAATTA                 >  SRR4302533.3455388/1‑94 (MQ=60)
                                                                               CCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTAT                <  SRR4302533.2797012/94‑1 (MQ=60)
                                                                               CCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTAT                >  SRR4302533.5816991/1‑94 (MQ=60)
                                                                               CCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTAT                <  SRR4302533.6281399/94‑1 (MQ=60)
                                                                               CCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTAT                >  SRR4302533.815035/1‑94 (MQ=60)
                                                                               CCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTAT                <  SRR4302533.1130099/94‑1 (MQ=60)
                                                                                 AACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAANTNATTC              >  SRR4302533.5729797/1‑94 (MQ=60)
                                                                                 AACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTC              <  SRR4302533.5062223/94‑1 (MQ=60)
                                                                                  ACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCC             <  SRR4302533.5343980/94‑1 (MQ=60)
                                                                                  ACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCC             <  SRR4302533.4738460/94‑1 (MQ=60)
                                                                                   CCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCG            <  SRR4302533.5091235/94‑1 (MQ=60)
                                                                                   CCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCG            >  SRR4302533.3818689/1‑94 (MQ=60)
                                                                                   CCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCG            <  SRR4302533.624764/94‑1 (MQ=60)
                                                                                    CGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTTCGG           >  SRR4302533.3718295/1‑94 (MQ=60)
                                                                                    CGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGG           <  SRR4302533.1313122/94‑1 (MQ=60)
                                                                                     GCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGG          <  SRR4302533.3848830/94‑1 (MQ=60)
                                                                                      CGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGGATTTATGTGGAAATTATTCCGGGA         >  SRR4302533.89965/1‑94 (MQ=60)
                                                                                      CGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGA         <  SRR4302533.5137323/94‑1 (MQ=60)
                                                                                      CGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGA         <  SRR4302533.909291/94‑1 (MQ=60)
                                                                                       GCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAA        <  SRR4302533.2218610/94‑1 (MQ=60)
                                                                                       GCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAA        <  SRR4302533.3644716/94‑1 (MQ=60)
                                                                                        CGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCCTTGATGTGGAAATTATTCCGGGCAT       >  SRR4302533.1177276/1‑94 (MQ=60)
                                                                                         GATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATT      <  SRR4302533.494208/94‑1 (MQ=60)
                                                                                           TTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGGATTGGTGTGGAAATTATTTCGGTGATTTT    >  SRR4302533.1060339/1‑94 (MQ=60)
                                                                                            TGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGAATTATTCtggggtgtttg   >  SRR4302533.3591319/1‑83 (MQ=60)
                                                                                             GCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTTCGGGAACTTTTC  >  SRR4302533.2468087/1‑94 (MQ=60)
                                                                                             GCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGC  <  SRR4302533.3613841/94‑1 (MQ=60)
                                                                                             |                                                                                             
GCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGC  >  NC_000913/4130646‑4130832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑