Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,146,393 A→G R484R (CGT→CGC)  yijP ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,146,3930AG92.1% 1019.3 / 59.4 379R484R (CGT→CGC) yijPhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (20/10);  new base G (168/181);  total (188/191)
Fisher's exact test for biased strand distribution p-value = 5.82e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-01

Only 100 of 399 total aligned reads displayed.

TATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAA  >  NC_000913/4146301‑4146482
                                                                                            |                                                                                         
  tttACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGcg                                                                                         <  1:7249722/93‑1 (MQ=255)
   ttACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGgcgc                                                                                        >  2:9444804/1‑93 (MQ=255)
   ttACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGgcgc                                                                                        >  2:1346241/1‑93 (MQ=255)
   ttACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGgcgc                                                                                        >  2:3963500/1‑93 (MQ=255)
    tACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGcgcg                                                                                       <  2:7672069/93‑1 (MQ=255)
      cGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTc                                                                                     <  1:9831572/93‑1 (MQ=255)
      cGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTc                                                                                     <  2:8652682/93‑1 (MQ=255)
      cGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTc                                                                                     <  1:7837499/93‑1 (MQ=255)
      cGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTc                                                                                     <  1:7572779/93‑1 (MQ=255)
      cGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTc                                                                                     <  2:7271876/93‑1 (MQ=255)
        aTCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCgg                                                                                   >  2:6717064/1‑93 (MQ=255)
        aTCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCgg                                                                                   >  1:3685074/1‑93 (MQ=255)
        aTCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCgg                                                                                   <  2:6188221/93‑1 (MQ=255)
         tCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCggg                                                                                  >  2:841708/1‑93 (MQ=255)
          cAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGt                                                                                 <  2:2428240/93‑1 (MQ=255)
          cAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTAAATATGGCGCGTCGGGt                                                                                 >  2:2822474/1‑93 (MQ=255)
           aaCATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGtt                                                                                >  1:939682/1‑93 (MQ=255)
           aaCATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGtt                                                                                <  2:6118501/93‑1 (MQ=255)
           aaCATCCTGCGAGAAATAACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGtt                                                                                <  1:3166004/93‑1 (MQ=255)
            aCATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTg                                                                               <  2:5898245/93‑1 (MQ=255)
            aCATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTg                                                                               >  2:8585461/1‑93 (MQ=255)
              aTCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTc                                                                             >  1:1537934/1‑93 (MQ=255)
               tCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTcc                                                                            >  1:4690420/1‑93 (MQ=255)
               tCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTcc                                                                            <  1:2128295/93‑1 (MQ=255)
               tCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTcc                                                                            <  2:3802036/93‑1 (MQ=255)
                ccTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCt                                                                           <  1:6118467/93‑1 (MQ=255)
                 cTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGAGCGTCGGGTTGTCCTc                                                                          >  2:4433359/1‑93 (MQ=255)
                  tGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCa                                                                         <  2:1783400/93‑1 (MQ=255)
                  cgCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCa                                                                         <  1:7476063/92‑1 (MQ=255)
                     gagaAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTa                                                                      >  1:1901328/1‑93 (MQ=255)
                     gagaAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTa                                                                      >  2:2549919/1‑93 (MQ=255)
                          aTCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGccc                                                                 >  2:9391277/1‑93 (MQ=255)
                           tCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGcccc                                                                >  1:5161167/1‑93 (MQ=255)
                             acagggATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGCTGTCCTCATTATGCCCCTg                                                              >  2:797825/4‑93 (MQ=255)
                              cGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGa                                                             >  1:7097431/1‑93 (MQ=255)
                               ggggATGAGTCGCTTGCCATTGTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAg                                                            <  2:7043409/93‑1 (MQ=255)
                                 ggATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTATCCTCATTACGCCCCTGAggt                                                          >  1:3299957/1‑91 (MQ=255)
                                  gATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGttt                                                         <  1:7399193/93‑1 (MQ=255)
                                  gATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGttt                                                         <  2:2043224/93‑1 (MQ=255)
                                   aTGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGtttt                                                        <  2:3016471/93‑1 (MQ=255)
                                    tGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTtg                                                       >  1:5917667/1‑93 (MQ=255)
                                     gAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTtgt                                                      <  2:4380186/93‑1 (MQ=255)
                                      aGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTtgtg                                                     >  1:2418257/1‑93 (MQ=255)
                                      aGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTtgtg                                                     <  2:6825460/93‑1 (MQ=255)
                                         cGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTgcgg                                                  <  2:9632820/93‑1 (MQ=255)
                                          gCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTgcggc                                                 >  2:4803980/1‑93 (MQ=255)
                                          gCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTgcggc                                                 >  2:875371/1‑93 (MQ=255)
                                          gCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGGTTTGTgcggg                                                 >  2:262512/1‑92 (MQ=255)
                                            ttGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTgcggcgg                                               >  2:9173568/1‑93 (MQ=255)
                                              gCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTgcggcggcg                                             <  2:5320127/93‑1 (MQ=255)
                                              gCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTgcggcggcg                                             >  1:8564238/1‑93 (MQ=255)
                                               ccATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCgt                                            <  2:4516398/93‑1 (MQ=255)
                                               ccATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCgt                                            <  1:658207/93‑1 (MQ=255)
                                                cATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCgtg                                           <  1:1157740/93‑1 (MQ=255)
                                                   ttttCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCg                                        >  1:10017483/1‑93 (MQ=255)
                                                     ttCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTa                                      >  1:573067/1‑93 (MQ=255)
                                                      tCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTaa                                     >  2:7435973/1‑93 (MQ=255)
                                                      tCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTaa                                     <  1:6976649/93‑1 (MQ=255)
                                                      tCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTaa                                     >  1:2010668/1‑93 (MQ=255)
                                                      tCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTaa                                     >  1:2339125/1‑93 (MQ=255)
                                                       cTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTaaa                                    <  1:8136601/93‑1 (MQ=255)
                                                        tGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAAc                                   <  2:1891301/93‑1 (MQ=255)
                                                         gACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAAcc                                  >  1:3099580/1‑93 (MQ=255)
                                                          aCGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACct                                 >  2:10906/1‑93 (MQ=255)
                                                             tCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTACACCTCtt                              >  1:8842052/1‑93 (MQ=255)
                                                             tCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCtt                              <  2:3271496/93‑1 (MQ=255)
                                                             tCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCtt                              <  1:6836708/93‑1 (MQ=255)
                                                             tCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCtt                              >  2:1520529/1‑93 (MQ=255)
                                                             tCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCtt                              >  2:7222432/1‑93 (MQ=255)
                                                               cacaGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCa                            <  1:2198708/93‑1 (MQ=255)
                                                                ccaggagGAACGGGATGGTGGACATATGGCGCGTGCGGTGGTCCTCAGTACGCCCCTGAGTTTTGTGCGGCGGCTTGTCGTAAACCTCTTCAc                           <  1:9679577/92‑1 (MQ=255)
                                                                acaGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCAc                           >  2:4151311/1‑93 (MQ=255)
                                                                   gcagGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGt                        <  2:4872587/93‑1 (MQ=255)
                                                                    cagGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTg                       <  2:8806170/93‑1 (MQ=255)
                                                                     agGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTgg                      >  2:6633245/1‑93 (MQ=255)
                                                                      ggAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGt                     >  2:8933463/1‑93 (MQ=255)
                                                                      ggAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGt                     >  2:6592395/1‑93 (MQ=255)
                                                                      ggAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGt                     <  2:5668901/93‑1 (MQ=255)
                                                                          cGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCaga                 >  1:7725198/1‑93 (MQ=255)
                                                                            gAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAgaga               <  2:1179294/93‑1 (MQ=255)
                                                                            gAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAgaga               <  1:130489/93‑1 (MQ=255)
                                                                            gAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAgaga               >  2:8288014/1‑93 (MQ=255)
                                                                             aaTGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGaa              <  1:7963926/93‑1 (MQ=255)
                                                                              aTGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGaaa             <  1:451358/93‑1 (MQ=255)
                                                                              aTGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGaaa             <  2:215318/93‑1 (MQ=255)
                                                                               tGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAAt            >  2:353112/1‑93 (MQ=255)
                                                                               tGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTAACCGGGGTCAGCGaaaa            >  1:2718706/1‑92 (MQ=255)
                                                                                ggTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATa           <  1:4052394/93‑1 (MQ=255)
                                                                                ggTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAAACTCTTCACCGTGGTCAGAGAAATa           >  1:4959273/1‑93 (MQ=255)
                                                                                 gtgtACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATaa          <  2:5061855/93‑1 (MQ=255)
                                                                                  tgtACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATaaa         >  1:172126/1‑93 (MQ=255)
                                                                                  tgtACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAAAAATaaa         >  1:4218964/1‑93 (MQ=255)
                                                                                   gtACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAAc        <  2:2257852/93‑1 (MQ=255)
                                                                                   gtACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAAc        <  1:8321387/93‑1 (MQ=255)
                                                                                    tACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAAcc       <  1:5386907/93‑1 (MQ=255)
                                                                                    tACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAAcc       <  1:6396856/93‑1 (MQ=255)
                                                                                     aCATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCa      >  1:3836783/1‑93 (MQ=255)
                                                                                     aCATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCa      >  2:6420812/1‑93 (MQ=255)
                                                                                       atatGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAgg    <  1:9094862/93‑1 (MQ=255)
                                                                                        tatGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGa   >  1:8996910/1‑93 (MQ=255)
                                                                                            |                                                                                         
TATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAA  >  NC_000913/4146301‑4146482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 214 total aligned reads displayed.
GTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAAACCG  >  NC_000913/4146300‑4146486
                                                                                             |                                                                                             
GTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGG                                                                                               <  SRR4302540.2785081/94‑1 (MQ=60)
GTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGG                                                                                               >  SRR4302540.3942054/1‑94 (MQ=60)
GTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGG                                                                                               >  SRR4302540.9519311/1‑94 (MQ=60)
GTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGG                                                                                               >  SRR4302540.2172606/1‑94 (MQ=60)
GTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGG                                                                                               >  SRR4302540.4553224/1‑94 (MQ=60)
GTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGG                                                                                               >  SRR4302540.6061099/1‑94 (MQ=60)
GTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGG                                                                                               >  SRR4302540.7977664/1‑94 (MQ=60)
 TATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGC                                                                                              >  SRR4302540.26086/1‑94 (MQ=60)
 TATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGC                                                                                              >  SRR4302540.2881313/1‑94 (MQ=60)
 TATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGC                                                                                              <  SRR4302540.4140836/94‑1 (MQ=60)
 TATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGC                                                                                              <  SRR4302540.7026333/94‑1 (MQ=60)
 TATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGC                                                                                              <  SRR4302540.9577326/94‑1 (MQ=60)
 TATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGAC                                                                                              <  SRR4302540.9089880/94‑1 (MQ=60)
  ATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCG                                                                                             <  SRR4302540.3624638/94‑1 (MQ=60)
  ATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCG                                                                                             <  SRR4302540.1231951/94‑1 (MQ=60)
  ATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCG                                                                                             <  SRR4302540.5418313/94‑1 (MQ=60)
  ATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCG                                                                                             >  SRR4302540.1763451/1‑94 (MQ=60)
  ATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATAAGACG                                                                                             >  SRR4302540.5744253/1‑94 (MQ=60)
   TTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGC                                                                                            <  SRR4302540.341009/94‑1 (MQ=60)
   TTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGC                                                                                            <  SRR4302540.7330618/94‑1 (MQ=60)
     taaaaaaaagaacatgggagaaatgaggggtgtgagtcttttctttTTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGT                                                                                          <  SRR4302540.8736237/48‑1 (MQ=60)
      ACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTC                                                                                         <  SRR4302540.927581/94‑1 (MQ=60)
       CGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCG                                                                                        <  SRR4302540.7929068/94‑1 (MQ=60)
       CGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCG                                                                                        <  SRR4302540.7657734/94‑1 (MQ=60)
       CGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCG                                                                                        <  SRR4302540.9946555/94‑1 (MQ=60)
       CGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCG                                                                                        <  SRR4302540.1543224/94‑1 (MQ=60)
        GATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGG                                                                                       >  SRR4302540.7786327/1‑94 (MQ=60)
        GATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGG                                                                                       >  SRR4302540.3725341/1‑94 (MQ=60)
        GATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGG                                                                                       >  SRR4302540.3725342/1‑94 (MQ=60)
         ATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGG                                                                                      <  SRR4302540.7491809/94‑1 (MQ=60)
           CAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTT                                                                                    >  SRR4302540.1029563/1‑94 (MQ=60)
           CAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTT                                                                                    >  SRR4302540.4825178/1‑94 (MQ=60)
           CAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTT                                                                                    >  SRR4302540.949510/1‑94 (MQ=60)
            AACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGCCGGGTTG                                                                                   <  SRR4302540.2054249/94‑1 (MQ=60)
            AACATCCTGCGAGAAATAACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTG                                                                                   <  SRR4302540.3200433/94‑1 (MQ=60)
            AACATCCTGCGAGAAATAACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTG                                                                                   <  SRR4302540.3200432/94‑1 (MQ=60)
             ACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGT                                                                                  >  SRR4302540.2196426/1‑94 (MQ=60)
              CATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTC                                                                                 >  SRR4302540.1554941/1‑94 (MQ=60)
              CATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGGGTACATATGGCGCGTCGGGTTGTC                                                                                 >  SRR4302540.2371038/1‑94 (MQ=60)
               ATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCC                                                                                >  SRR4302540.11217/1‑94 (MQ=60)
               ATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCC                                                                                >  SRR4302540.3545758/1‑94 (MQ=60)
               ATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCC                                                                                >  SRR4302540.4740574/1‑94 (MQ=60)
               ATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCC                                                                                <  SRR4302540.7130894/94‑1 (MQ=60)
                TCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCT                                                                               <  SRR4302540.5498625/94‑1 (MQ=60)
                 CCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTC                                                                              <  SRR4302540.6186609/94‑1 (MQ=60)
                  CTGCGAGAGATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGACCGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCA                                                                             <  SRR4302540.3786708/94‑1 (MQ=60)
                   TGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCAT                                                                            >  SRR4302540.5969408/1‑94 (MQ=60)
                   TGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCAT                                                                            <  SRR4302540.7559496/94‑1 (MQ=60)
                   TGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCAT                                                                            <  SRR4302540.8665108/94‑1 (MQ=60)
                   CGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCAT                                                                            <  SRR4302540.7559495/94‑1 (MQ=60)
                    CCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATT                                                                           <  SRR4302540.2143004/94‑1 (MQ=60)
                     CGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTA                                                                          <  SRR4302540.10100812/94‑1 (MQ=60)
                     CGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTA                                                                          >  SRR4302540.1922119/1‑94 (MQ=60)
                      GAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTAC                                                                         >  SRR4302540.7940536/1‑94 (MQ=60)
                      GAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTAC                                                                         <  SRR4302540.7042645/94‑1 (MQ=60)
                       AGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACG                                                                        <  SRR4302540.2725000/94‑1 (MQ=60)
                       AGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACG                                                                        >  SRR4302540.6738191/1‑94 (MQ=60)
                         AAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCC                                                                      <  SRR4302540.5013053/94‑1 (MQ=60)
                          AATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCC                                                                     <  SRR4302540.4811602/94‑1 (MQ=60)
                          AATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCC                                                                     <  SRR4302540.9054689/94‑1 (MQ=60)
                          AATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCC                                                                     <  SRR4302540.5422192/94‑1 (MQ=60)
                           ATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCC                                                                    >  SRR4302540.5217275/1‑94 (MQ=60)
                           ATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCC                                                                    >  SRR4302540.1344229/1‑94 (MQ=60)
                            TCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCT                                                                   <  SRR4302540.8139375/94‑1 (MQ=60)
                              ACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGA                                                                 >  SRR4302540.1271236/1‑94 (MQ=60)
                              ACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGA                                                                 >  SRR4302540.7176409/1‑94 (MQ=60)
                               CGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAG                                                                <  SRR4302540.5700721/94‑1 (MQ=60)
                                GGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGT                                                               >  SRR4302540.2444033/1‑94 (MQ=60)
                                GGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGT                                                               >  SRR4302540.1920872/1‑94 (MQ=60)
                                GGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGT                                                               >  SRR4302540.6870285/1‑94 (MQ=60)
                                 GGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTT                                                              <  SRR4302540.5977469/94‑1 (MQ=60)
                                 GGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTATCCTCATTACGCCCCTGAGGT                                                              >  SRR4302540.3336080/1‑94 (MQ=60)
                                  GGATGAGTCGCTTGCCATTTTTCTGACGACCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTT                                                             >  SRR4302540.3183540/1‑94 (MQ=60)
                                   GATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTT                                                            <  SRR4302540.8839194/94‑1 (MQ=60)
                                   GATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTT                                                            <  SRR4302540.7481628/94‑1 (MQ=60)
                                   GATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGGCGGGTTGTCCTCATTACGCCCCTGAGTTTT                                                            >  SRR4302540.9594714/1‑94 (MQ=60)
                                    ATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTG                                                           >  SRR4302540.5983040/1‑94 (MQ=60)
                                    ATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTG                                                           <  SRR4302540.1684721/94‑1 (MQ=60)
                                    ATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTG                                                           >  SRR4302540.300103/1‑94 (MQ=60)
                                    ATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTG                                                           >  SRR4302540.7854151/1‑94 (MQ=60)
                                     TGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGT                                                          <  SRR4302540.3255383/94‑1 (MQ=60)
                                     TGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGT                                                          <  SRR4302540.7120496/94‑1 (MQ=60)
                                     TGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGT                                                          >  SRR4302540.3791371/1‑94 (MQ=60)
                                     TGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGT                                                          <  SRR4302540.4157196/94‑1 (MQ=60)
                                     TGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCCCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGT                                                          <  SRR4302540.7138118/94‑1 (MQ=60)
                                      GAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTG                                                         >  SRR4302540.2444321/1‑94 (MQ=60)
                                       AGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGC                                                        <  SRR4302540.544159/94‑1 (MQ=60)
                                       AGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGC                                                        >  SRR4302540.3198700/1‑94 (MQ=60)
                                       AGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGC                                                        <  SRR4302540.544158/94‑1 (MQ=60)
                                        GTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCG                                                       <  SRR4302540.7466472/94‑1 (MQ=60)
                                           GCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCG                                                    <  SRR4302540.4839814/94‑1 (MQ=60)
                                            CTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGG                                                   <  SRR4302540.3947337/94‑1 (MQ=60)
                                            CTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGGTGTCCTCATTACGCCCCTGAGTTTTGGGCGGCGG                                                   >  SRR4302540.1237135/1‑94 (MQ=60)
                                            CGTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGG                                                   <  SRR4302540.3224478/94‑1 (MQ=60)
                                             TTGCCTTTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGC                                                  <  SRR4302540.3782047/94‑1 (MQ=60)
                                             TTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGCGTTTTGTGCGGCGGC                                                  >  SRR4302540.9537120/1‑94 (MQ=60)
                                             TTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGC                                                  >  SRR4302540.6420441/1‑94 (MQ=60)
                                                CCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTG                                               <  SRR4302540.665231/94‑1 (MQ=60)
                                                 CATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGT                                              <  SRR4302540.1169781/94‑1 (MQ=60)
                                                 CATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGT                                              <  SRR4302540.720853/94‑1 (MQ=60)
                                                  ATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTC                                             <  SRR4302540.613498/94‑1 (MQ=60)
                                                  ATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTC                                             >  SRR4302540.7929319/1‑94 (MQ=60)
                                                  ATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTC                                             >  SRR4302540.9196560/1‑94 (MQ=60)
                                                  ATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTC                                             >  SRR4302540.8328947/1‑94 (MQ=60)
                                                  ATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTC                                             >  SRR4302540.829537/1‑94 (MQ=60)
                                                  ATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGGTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGGC                                             >  SRR4302540.7806322/1‑94 (MQ=60)
                                                  ATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTC                                             >  SRR4302540.2216946/1‑94 (MQ=60)
                                                   TTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCG                                            >  SRR4302540.10134081/1‑94 (MQ=60)
                                                     TTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTA                                          >  SRR4302540.579288/1‑94 (MQ=60)
                                                     TTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTA                                          <  SRR4302540.4050456/94‑1 (MQ=60)
                                                      TTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCGTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAA                                         <  SRR4302540.1970901/94‑1 (MQ=60)
                                                      TTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAA                                         <  SRR4302540.8441295/94‑1 (MQ=60)
                                                      TTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAA                                         >  SRR4302540.4336062/1‑94 (MQ=60)
                                                      TTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAA                                         >  SRR4302540.7274341/1‑94 (MQ=60)
                                                      TTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAA                                         >  SRR4302540.2032410/1‑94 (MQ=60)
                                                      TTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAA                                         >  SRR4302540.2364331/1‑94 (MQ=60)
                                                       TCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAA                                        <  SRR4302540.7054039/94‑1 (MQ=60)
                                                        CTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAAC                                       <  SRR4302540.8231035/94‑1 (MQ=60)
                                                        CTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAAC                                       <  SRR4302540.2754563/94‑1 (MQ=60)
                                                        CTGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAAC                                       <  SRR4302540.785875/94‑1 (MQ=60)
                                                         TGACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACC                                      >  SRR4302540.3133535/1‑94 (MQ=60)
                                                          GACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCT                                     <  SRR4302540.7288177/94‑1 (MQ=60)
                                                          GACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCT                                     <  SRR4302540.9588452/94‑1 (MQ=60)
                                                          GACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCT                                     <  SRR4302540.2325646/94‑1 (MQ=60)
                                                          GACGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCT                                     <  SRR4302540.6140871/94‑1 (MQ=60)
                                                           ACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGCTTTGTGCGGAGGCGTGTCGTAAACCTC                                    >  SRR4302540.7046206/1‑94 (MQ=60)
                                                            CGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCT                                   <  SRR4302540.9010363/94‑1 (MQ=60)
                                                            CGTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCT                                   >  SRR4302540.640226/1‑94 (MQ=60)
                                                             GTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTACACCTCTT                                  >  SRR4302540.8944329/1‑94 (MQ=60)
                                                             GTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTT                                  >  SRR4302540.7785419/1‑94 (MQ=60)
                                                             GTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTT                                  >  SRR4302540.9502234/1‑94 (MQ=60)
                                                             GTCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTT                                  >  SRR4302540.9852253/1‑94 (MQ=60)
                                                             GTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTT                                  >  SRR4302540.382950/1‑94 (MQ=60)
                                                              TCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTC                                 <  SRR4302540.6699599/94‑1 (MQ=60)
                                                              TCCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTC                                 <  SRR4302540.6912430/94‑1 (MQ=60)
                                                               CCACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCA                                >  SRR4302540.6166635/1‑94 (MQ=60)
                                                                CACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCAC                               <  SRR4302540.2222450/94‑1 (MQ=60)
                                                                CACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCAC                               >  SRR4302540.4569648/1‑94 (MQ=60)
                                                                CACAGCAGGAACGGAATGGTGTACATATGGCGCAACCGGATGACCTCATTACGCCCCTGAGATTTGTGCGGAGGAGTGTCGTAAACATCTTCAC                               >  SRR4302540.1259378/1‑94 (MQ=42)
                                                                 ccaggAGGAACGGGATGGTGGACATATGGCGCGTGCGGTGGTCCTCAGTACGCCCCTGAGTTTTGTGCGGCGGCTTGTCGTAAACCTCTTCACC                              <  SRR4302540.9793464/89‑1 (MQ=55)
                                                                 CCAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACC                              <  SRR4302540.2478563/94‑1 (MQ=60)
                                                                 ACAGCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACC                              <  SRR4302540.7586491/94‑1 (MQ=60)
                                                                  CAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCG                             >  SRR4302540.5208859/1‑94 (MQ=60)
                                                                  CAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCG                             >  SRR4302540.5208858/1‑94 (MQ=60)
                                                                   AGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGT                            >  SRR4302540.7527882/1‑94 (MQ=60)
                                                                    GCAGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTG                           >  SRR4302540.6502432/1‑94 (MQ=60)
                                                                      AGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGT                         <  SRR4302540.4533235/94‑1 (MQ=60)
                                                                      AGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGT                         >  SRR4302540.1202108/1‑94 (MQ=60)
                                                                      AGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAAACTCTTCACCGTGGT                         >  SRR4302540.6508045/1‑94 (MQ=60)
                                                                      AGGAACGGAATGGTGTACATATGGCGCGTCGGGTTGGCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGC                         >  SRR4302540.8624133/1‑94 (MQ=60)
                                                                       GGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTC                        >  SRR4302540.1804822/1‑94 (MQ=60)
                                                                       GGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTC                        >  SRR4302540.9900786/1‑94 (MQ=60)
                                                                       GGAACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTC                        <  SRR4302540.2258979/94‑1 (MQ=60)
                                                                         AACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAG                      >  SRR4302540.6579256/1‑94 (MQ=60)
                                                                          ACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGA                     >  SRR4302540.7815322/1‑94 (MQ=60)
                                                                          ACGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGA                     <  SRR4302540.2012839/94‑1 (MQ=60)
                                                                           CGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAG                    >  SRR4302540.7337982/1‑94 (MQ=60)
                                                                           CGGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAG                    <  SRR4302540.9144304/94‑1 (MQ=60)
                                                                            GGAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGA                   >  SRR4302540.8649582/1‑94 (MQ=60)
                                                                             GAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAA                  <  SRR4302540.131862/94‑1 (MQ=60)
                                                                             GAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAA                  <  SRR4302540.4163838/94‑1 (MQ=60)
                                                                             GAATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAA                  <  SRR4302540.8145075/94‑1 (MQ=60)
                                                                              AATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAA                 <  SRR4302540.1390480/94‑1 (MQ=60)
                                                                              AATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAA                 <  SRR4302540.8056741/94‑1 (MQ=60)
                                                                               ATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAAT                <  SRR4302540.224372/94‑1 (MQ=60)
                                                                               ATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAAT                <  SRR4302540.456167/94‑1 (MQ=60)
                                                                               ATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAAT                >  SRR4302540.2426118/1‑94 (MQ=60)
                                                                               ATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTAACCGGGGTCAGCGAAAA                >  SRR4302540.2748017/1‑94 (MQ=60)
                                                                               ATGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGCCGTGTCGTAAACCTCTTCACCGTGGcgagagaaaa                >  SRR4302540.2748018/1‑84 (MQ=60)
                                                                                TGGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAAACTCTTCACCGTGGTCAGAGAAATA               >  SRR4302540.5012886/1‑94 (MQ=60)
                                                                                 GGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAA              <  SRR4302540.4096085/94‑1 (MQ=60)
                                                                                 GGTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAA              <  SRR4302540.4407571/94‑1 (MQ=60)
                                                                                  GTGTCCATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAA             <  SRR4302540.9908903/94‑1 (MQ=60)
                                                                                  GTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAA             >  SRR4302540.4264893/1‑94 (MQ=60)
                                                                                  GTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAA             >  SRR4302540.173846/1‑94 (MQ=60)
                                                                                  GTGTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAAAAATAAA             >  SRR4302540.4264894/1‑94 (MQ=60)
                                                                                  GTGTACATATGGCGCGTCGGGTTGTCCTCATTACACCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAA             >  SRR4302540.1624354/1‑94 (MQ=60)
                                                                                  GTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAA             >  SRR4302540.7468480/1‑94 (MQ=60)
                                                                                    GTACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACC           <  SRR4302540.8417978/94‑1 (MQ=60)
                                                                                     TACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCA          >  SRR4302540.3878518/1‑94 (MQ=60)
                                                                                     TACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCA          <  SRR4302540.6468069/94‑1 (MQ=60)
                                                                                      ACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAG         <  SRR4302540.5803828/94‑1 (MQ=60)
                                                                                      ACATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAG         <  SRR4302540.8088296/94‑1 (MQ=60)
                                                                                       CATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGG        >  SRR4302540.461329/1‑94 (MQ=60)
                                                                                       CATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGG        <  SRR4302540.2034685/94‑1 (MQ=60)
                                                                                        ATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGA       >  SRR4302540.9104840/1‑94 (MQ=60)
                                                                                        ATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGA       >  SRR4302540.9104837/1‑94 (MQ=60)
                                                                                        ATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGA       <  SRR4302540.9204130/94‑1 (MQ=60)
                                                                                        ATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGA       <  SRR4302540.9450531/94‑1 (MQ=60)
                                                                                        ATATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGA       >  SRR4302540.7912729/1‑94 (MQ=60)
                                                                                         TATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAA      <  SRR4302540.199009/94‑1 (MQ=60)
                                                                                         TATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAA      >  SRR4302540.2222295/1‑94 (MQ=60)
                                                                                         TATGGCGCGTCGGGGTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAA      <  SRR4302540.7581485/94‑1 (MQ=60)
                                                                                          ATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAAA     <  SRR4302540.8355210/94‑1 (MQ=60)
                                                                                          ATGGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAAA     >  SRR4302540.8044930/1‑94 (MQ=60)
                                                                                            GGCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAAACC   >  SRR4302540.9189359/1‑94 (MQ=60)
                                                                                             GCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAAACCG  <  SRR4302540.5102406/94‑1 (MQ=60)
                                                                                             GCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAAACCG  <  SRR4302540.5642320/94‑1 (MQ=60)
                                                                                             GCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAAACCG  <  SRR4302540.8864948/94‑1 (MQ=60)
                                                                                             GCGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAAACCG  <  SRR4302540.2822836/94‑1 (MQ=60)
                                                                                             |                                                                                             
GTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGACGCGTCGGGTTGTCCTCATTACGCCCCTGAGTTTTGTGCGGCGGCGTGTCGTAAACCTCTTCACCGTGGTCAGAGAAATAAACCAGGAAACCG  >  NC_000913/4146300‑4146486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑