Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,198,118 A→G D87G (GAC→GGC)  hupA → DNA‑binding protein HU‑alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,198,1180AG99.8% 1570.7 / NA 487D87G (GAC→GGC) hupADNA‑binding protein HU‑alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (218/268);  minor base C (0/1);  total (218/269)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 497 total aligned reads displayed.

AGCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGACGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGC  >  NC_000913/4198028‑4198207
                                                                                          |                                                                                         
aGCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCg                                                                                         >  2:4998657/1‑93 (MQ=255)
 gCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGgcgc                                                                                        <  1:624581/93‑1 (MQ=255)
      cTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTta                                                                                   >  2:4259793/1‑93 (MQ=255)
      cTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTta                                                                                   <  1:3003025/93‑1 (MQ=255)
       tGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTtaa                                                                                  <  1:5445687/93‑1 (MQ=255)
       tGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCCCAGTtaa                                                                                  >  2:6788095/1‑93 (MQ=255)
        ggCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTGTCTGGCAAGGCACTGAAAGGCGCAGTtaag                                                                                 <  1:1385008/93‑1 (MQ=255)
         gCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTtaagt                                                                                <  2:3949327/93‑1 (MQ=255)
           cGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTtaagtaa                                                                              <  2:974793/93‑1 (MQ=255)
           cGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTtaagtaa                                                                              <  1:804983/93‑1 (MQ=255)
           cGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTtaagtaa                                                                              >  1:8417460/1‑93 (MQ=255)
            gCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTtaagtaag                                                                             >  2:7818141/1‑93 (MQ=255)
            gCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTtaagtaag                                                                             >  1:7187207/1‑93 (MQ=255)
             cAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGa                                                                            <  2:9876732/93‑1 (MQ=255)
             cAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGa                                                                            <  2:9128350/93‑1 (MQ=255)
              aaCCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGAt                                                                           <  2:4812116/93‑1 (MQ=255)
              aaCCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGAt                                                                           <  2:872190/93‑1 (MQ=255)
              aaCCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGAt                                                                           >  2:4448516/1‑93 (MQ=255)
              aaCCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGAt                                                                           <  2:3086398/93‑1 (MQ=255)
                 ccGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGc                                                                        >  2:6656716/1‑93 (MQ=255)
                 ccGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGc                                                                        >  1:4619695/1‑93 (MQ=255)
                  cGCAGCCCGGTAAAGAAATCAAAATTGCCGCATCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCg                                                                       <  1:2961030/93‑1 (MQ=255)
                  cGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCg                                                                       >  2:5641045/1‑93 (MQ=255)
                  cGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCg                                                                       <  2:3373042/93‑1 (MQ=255)
                  cGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCg                                                                       <  2:7599972/93‑1 (MQ=255)
                   gCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGt                                                                      <  1:3251944/93‑1 (MQ=255)
                     aGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTgg                                                                    <  1:2635610/93‑1 (MQ=255)
                         cGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGt                                                                <  2:2203600/93‑1 (MQ=255)
                         cGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGt                                                                >  1:971320/1‑93 (MQ=255)
                         cGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGt                                                                >  1:38751/1‑93 (MQ=255)
                          ggTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTg                                                               >  2:135572/1‑93 (MQ=255)
                          ggTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTg                                                               >  1:407038/1‑93 (MQ=255)
                            tAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGaa                                                             <  2:5228876/93‑1 (MQ=255)
                             aaaGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAAc                                                            <  1:5821292/93‑1 (MQ=255)
                             aaaGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAAc                                                            >  2:9408256/1‑93 (MQ=255)
                             aaaGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAAc                                                            >  1:6602587/1‑93 (MQ=255)
                             aaaGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAAc                                                            >  2:7979149/1‑93 (MQ=255)
                                gAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGt                                                         >  2:7395557/1‑93 (MQ=255)
                                 aaaTCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGtt                                                        <  1:4482368/93‑1 (MQ=255)
                                 aaaTCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGtt                                                        >  2:8813373/1‑93 (MQ=255)
                                  aaTCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGttt                                                       <  1:9272483/93‑1 (MQ=255)
                                   aTCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGtttt                                                      <  1:9595093/93‑1 (MQ=255)
                                   aTCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGtttt                                                      >  2:9609928/1‑93 (MQ=255)
                                    tCAAAATTGACGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTa                                                     <  2:8361761/93‑1 (MQ=255)
                                     cAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGGTTTaa                                                    <  2:9001562/93‑1 (MQ=255)
                                      aaaaTTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAAc                                                   <  2:278922/93‑1 (MQ=255)
                                        aaTTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGa                                                 >  1:5097891/1‑93 (MQ=255)
                                        aaTTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGa                                                 >  1:6557394/1‑93 (MQ=255)
                                        aaTTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGa                                                 >  2:1233566/1‑93 (MQ=255)
                                         aTTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGaa                                                <  1:2130113/93‑1 (MQ=255)
                                         aTTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGaa                                                <  1:7395558/93‑1 (MQ=255)
                                         aTTCCCGCATCTAACGTACCGGCATTTGGTTCGGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGaa                                                <  1:2413683/93‑1 (MQ=255)
                                            gCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAggg                                             >  1:6385283/1‑93 (MQ=255)
                                             ccGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAgggg                                            <  2:813772/93‑1 (MQ=255)
                                             ccGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAgggg                                            <  2:1001798/93‑1 (MQ=255)
                                              cGCAGCTAACGTACCTGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGggt                                           <  2:6280507/93‑1 (MQ=255)
                                              cGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGggt                                           <  2:3689296/93‑1 (MQ=255)
                                               gCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGggtg                                          >  2:4552662/1‑93 (MQ=255)
                                                cAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGggtgg                                         <  1:1654582/93‑1 (MQ=255)
                                                  gCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGtt                                       <  2:1820605/93‑1 (MQ=255)
                                                  gCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGtt                                       <  1:7642845/93‑1 (MQ=255)
                                                     aaCGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGCAGGGGTGGTTTCa                                    >  2:6218974/1‑93 (MQ=255)
                                                     aaCGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCa                                    >  1:1884181/1‑93 (MQ=255)
                                                      aCGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCAc                                   >  2:720045/1‑93 (MQ=255)
                                                         tACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCAccac                                >  2:1216264/1‑91 (MQ=255)
                                                           ccGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCtt                              <  2:6944581/93‑1 (MQ=255)
                                                            cGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCttt                             <  2:4109238/93‑1 (MQ=255)
                                                             ggCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCtttt                            <  1:9029831/93‑1 (MQ=255)
                                                             ggCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCtttt                            <  1:2308564/93‑1 (MQ=255)
                                                             ggCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCtttt                            >  1:3737616/1‑93 (MQ=255)
                                                              gCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTg                           >  1:10011664/1‑93 (MQ=255)
                                                               cATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGt                          >  1:4686454/1‑93 (MQ=255)
                                                                aTTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTc                         <  1:4298384/93‑1 (MQ=255)
                                                                 tttGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCt                        >  1:8725613/1‑93 (MQ=255)
                                                                  ttGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCtt                       >  2:5391691/1‑93 (MQ=255)
                                                                  ttGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCtt                       >  2:1157700/1‑93 (MQ=255)
                                                                  ttGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCtt                       <  1:9844957/93‑1 (MQ=255)
                                                                  ttGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCtt                       <  1:7493970/93‑1 (MQ=255)
                                                                   tGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCttt                      <  1:6088442/93‑1 (MQ=255)
                                                                    gTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTc                     >  1:888863/1‑93 (MQ=255)
                                                                      ttCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTg                   <  1:3577397/93‑1 (MQ=255)
                                                                       tCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTgg                  <  2:3160323/93‑1 (MQ=255)
                                                                        cTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGCTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGc                 >  1:9157091/1‑93 (MQ=255)
                                                                         tGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCg                >  1:6757682/1‑93 (MQ=255)
                                                                           gCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTc              <  1:1276663/93‑1 (MQ=255)
                                                                           gCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTc              >  1:8567750/1‑93 (MQ=255)
                                                                             aaGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGa            <  2:6394346/93‑1 (MQ=255)
                                                                              aGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGAt           >  2:33046/1‑93 (MQ=255)
                                                                                gCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCa         <  1:5293024/93‑1 (MQ=255)
                                                                                gCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCa         <  1:1626496/93‑1 (MQ=255)
                                                                                 cACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCAt        >  1:3472970/1‑93 (MQ=255)
                                                                                   cTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTg      >  1:8254756/1‑93 (MQ=255)
                                                                                    tGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGa     <  2:6479353/93‑1 (MQ=255)
                                                                                    tGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGa     <  2:5842121/93‑1 (MQ=255)
                                                                                    tGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGa     >  2:2605809/1‑93 (MQ=255)
                                                                                     gAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGAt    >  2:6094584/1‑93 (MQ=255)
                                                                                     gAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGAt    >  1:7849564/1‑93 (MQ=255)
                                                                                     gAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGAt    <  2:3530552/93‑1 (MQ=255)
                                                                                     gAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGAt    >  2:1560237/1‑93 (MQ=255)
                                                                                       aaGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATgc  <  1:3410934/93‑1 (MQ=255)
                                                                                          |                                                                                         
AGCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGACGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGC  >  NC_000913/4198028‑4198207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 266 total aligned reads displayed.
CTGAGCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGACGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGG  >  NC_000913/4198025‑4198210
                                                                                             |                                                                                            
CTGAGCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGG                                                                                              <  SRR4302540.3771529/94‑1 (MQ=60)
CTGAGCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGG                                                                                              >  SRR4302540.6844882/1‑94 (MQ=60)
CTGAGCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGG                                                                                              >  SRR4302540.1177818/1‑94 (MQ=60)
CTGAGCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGG                                                                                              <  SRR4302540.308523/94‑1 (MQ=60)
 TGAGCGTACTGGCCGCAACCCGCAGACCGGTAAAGCAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGC                                                                                             >  SRR4302540.4336964/1‑94 (MQ=60)
 TGAGCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGC                                                                                             >  SRR4302540.5040777/1‑94 (MQ=60)
 TGAGCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGC                                                                                             >  SRR4302540.1771399/1‑94 (MQ=60)
 TGAGCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGC                                                                                             <  SRR4302540.6049668/94‑1 (MQ=60)
 CGAGCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTGCCGGCATTTGTTTCTGGCAAGGCACTGAAAGGC                                                                                             <  SRR4302540.6772340/94‑1 (MQ=60)
  GAGCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCG                                                                                            >  SRR4302540.8978548/1‑94 (MQ=60)
   AGCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGC                                                                                           <  SRR4302540.6184873/94‑1 (MQ=60)
    GCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCA                                                                                          >  SRR4302540.249926/1‑94 (MQ=60)
    GCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCA                                                                                          <  SRR4302540.631229/94‑1 (MQ=60)
     CGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAG                                                                                         >  SRR4302540.8604089/1‑94 (MQ=60)
      GTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGT                                                                                        >  SRR4302540.7653828/1‑94 (MQ=60)
      GTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGT                                                                                        <  SRR4302540.2932951/94‑1 (MQ=60)
      GTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGT                                                                                        >  SRR4302540.8997923/1‑94 (MQ=60)
        ACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTA                                                                                      >  SRR4302540.846770/1‑94 (MQ=60)
         CTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAA                                                                                     <  SRR4302540.1125496/94‑1 (MQ=60)
          TGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAG                                                                                    <  SRR4302540.5505615/94‑1 (MQ=60)
          TGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAG                                                                                    <  SRR4302540.8881670/94‑1 (MQ=60)
          TGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAG                                                                                    <  SRR4302540.1370378/94‑1 (MQ=60)
           GGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTGTCTGGCAAGGCACTGAAAGGCGCAGTTAAGT                                                                                   <  SRR4302540.1399811/94‑1 (MQ=60)
            GCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTA                                                                                  <  SRR4302540.87157/94‑1 (MQ=60)
             CCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAA                                                                                 >  SRR4302540.8515167/1‑94 (MQ=60)
              CGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAG                                                                                <  SRR4302540.3808771/94‑1 (MQ=60)
              CGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAG                                                                                <  SRR4302540.813536/94‑1 (MQ=60)
              CGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAG                                                                                >  SRR4302540.6287174/1‑94 (MQ=60)
              CGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAG                                                                                <  SRR4302540.7654580/94‑1 (MQ=60)
              CGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAG                                                                                >  SRR4302540.7267322/1‑94 (MQ=60)
               GCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGA                                                                               >  SRR4302540.2177967/1‑94 (MQ=60)
                CAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGAT                                                                              <  SRR4302540.2123626/94‑1 (MQ=60)
                 AACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATT                                                                             >  SRR4302540.9775020/1‑94 (MQ=60)
                 AACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATT                                                                             <  SRR4302540.10151009/94‑1 (MQ=60)
                 AACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATT                                                                             >  SRR4302540.6474227/1‑94 (MQ=60)
                   CCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGC                                                                           >  SRR4302540.4669222/1‑94 (MQ=60)
                    CCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCG                                                                          <  SRR4302540.9161821/94‑1 (MQ=60)
                    CCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCG                                                                          >  SRR4302540.5107086/1‑94 (MQ=60)
                    CCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCG                                                                          >  SRR4302540.7296880/1‑94 (MQ=60)
                     CGCAGCCCGGTAAAGAAATCAAAATTGCCGCATCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGT                                                                         <  SRR4302540.2993677/94‑1 (MQ=60)
                     CGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGT                                                                         >  SRR4302540.932954/1‑94 (MQ=60)
                     CGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGT                                                                         >  SRR4302540.10011108/1‑94 (MQ=60)
                     CGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGT                                                                         >  SRR4302540.9113233/1‑94 (MQ=60)
                      GCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTG                                                                        <  SRR4302540.3287378/94‑1 (MQ=60)
                        AGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGC                                                                      <  SRR4302540.2664146/94‑1 (MQ=60)
                        AGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGC                                                                      <  SRR4302540.9556531/94‑1 (MQ=60)
                        AGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGC                                                                      >  SRR4302540.1670896/1‑94 (MQ=60)
                         GACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCA                                                                     >  SRR4302540.9303443/1‑94 (MQ=60)
                          ACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAG                                                                    <  SRR4302540.3762116/94‑1 (MQ=60)
                          ACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAG                                                                    >  SRR4302540.1600625/1‑94 (MQ=60)
                           CCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAGGGCGCAGTTAAGTAAGATTGCGTGGCAGT                                                                   <  SRR4302540.8448715/94‑1 (MQ=60)
                           CCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGT                                                                   <  SRR4302540.8448714/94‑1 (MQ=60)
                           CCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGT                                                                   <  SRR4302540.9349365/94‑1 (MQ=60)
                           CCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGT                                                                   >  SRR4302540.39148/1‑94 (MQ=60)
                           CCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGT                                                                   >  SRR4302540.8179560/1‑94 (MQ=60)
                           CCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGT                                                                   >  SRR4302540.1415425/1‑94 (MQ=60)
                           CCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGT                                                                   <  SRR4302540.707967/94‑1 (MQ=60)
                           CCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGT                                                                   >  SRR4302540.8584985/1‑94 (MQ=60)
                            CGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTG                                                                  >  SRR4302540.411296/1‑94 (MQ=60)
                              GTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAA                                                                <  SRR4302540.7486584/94‑1 (MQ=60)
                               TAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAAC                                                               <  SRR4302540.241612/94‑1 (MQ=60)
                               TAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAAC                                                               <  SRR4302540.983968/94‑1 (MQ=60)
                               TAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAAC                                                               >  SRR4302540.6675702/1‑94 (MQ=60)
                               TAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAAC                                                               >  SRR4302540.6675703/1‑94 (MQ=60)
                               TAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAAC                                                               >  SRR4302540.1792999/1‑94 (MQ=60)
                               TAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAAA                                                               >  SRR4302540.5623858/1‑94 (MQ=60)
                                 AAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAG                                                             <  SRR4302540.5322494/94‑1 (MQ=60)
                                  ATAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGT                                                            <  SRR4302540.7964881/94‑1 (MQ=60)
                                  AGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGT                                                            <  SRR4302540.4614563/94‑1 (MQ=60)
                                   GAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTT                                                           <  SRR4302540.3832618/94‑1 (MQ=60)
                                   GAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTT                                                           >  SRR4302540.1998187/1‑94 (MQ=60)
                                   GAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTT                                                           >  SRR4302540.7199060/1‑94 (MQ=60)
                                   AAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTT                                                           <  SRR4302540.4683173/94‑1 (MQ=60)
                                    AAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTT                                                          <  SRR4302540.4530982/94‑1 (MQ=60)
                                    AAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTT                                                          <  SRR4302540.4778693/94‑1 (MQ=60)
                                    AAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTT                                                          >  SRR4302540.348019/1‑94 (MQ=60)
                                    AAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTT                                                          <  SRR4302540.5409335/94‑1 (MQ=60)
                                     TATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTT                                                         >  SRR4302540.2800132/1‑94 (MQ=60)
                                     AATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTT                                                         <  SRR4302540.9383354/94‑1 (MQ=60)
                                      ATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTA                                                        <  SRR4302540.7703938/94‑1 (MQ=60)
                                       TCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAA                                                       <  SRR4302540.6384484/94‑1 (MQ=60)
                                        CAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAAC                                                      <  SRR4302540.6533861/94‑1 (MQ=60)
                                        CAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAAC                                                      <  SRR4302540.1667275/94‑1 (MQ=60)
                                         AAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACG                                                     <  SRR4302540.3223536/94‑1 (MQ=60)
                                         AAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACG                                                     <  SRR4302540.4029974/94‑1 (MQ=60)
                                          AAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGA                                                    <  SRR4302540.9980067/94‑1 (MQ=60)
                                          AAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGA                                                    >  SRR4302540.6630102/1‑94 (MQ=60)
                                          AAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGA                                                    >  SRR4302540.5153059/1‑94 (MQ=60)
                                          AAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGA                                                    >  SRR4302540.4259489/1‑94 (MQ=60)
                                          AAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGA                                                    >  SRR4302540.3846049/1‑94 (MQ=60)
                                           AATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAACGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAA                                                   >  SRR4302540.4161831/1‑94 (MQ=60)
                                           AATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAA                                                   >  SRR4302540.3268551/1‑94 (MQ=60)
                                            ATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAG                                                  <  SRR4302540.7477961/94‑1 (MQ=60)
                                            ATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAG                                                  <  SRR4302540.1335147/94‑1 (MQ=60)
                                            ATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAG                                                  <  SRR4302540.2152968/94‑1 (MQ=60)
                                            ATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAG                                                  <  SRR4302540.2439698/94‑1 (MQ=60)
                                            ATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAG                                                  <  SRR4302540.2566980/94‑1 (MQ=60)
                                            ATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAG                                                  >  SRR4302540.3762445/1‑94 (MQ=60)
                                            ATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAG                                                  <  SRR4302540.7477962/94‑1 (MQ=60)
                                              TGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGG                                                <  SRR4302540.9724029/94‑1 (MQ=60)
                                              TGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGG                                                <  SRR4302540.8880488/94‑1 (MQ=60)
                                                gcggaggctaccgaccgggcttttgTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTGACGAAGGGGT                                              <  SRR4302540.4672440/69‑1 (MQ=60)
                                                CCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGT                                              <  SRR4302540.6052037/94‑1 (MQ=60)
                                                CCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGT                                              <  SRR4302540.6785142/94‑1 (MQ=60)
                                                CCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGG                                              >  SRR4302540.3034555/1‑94 (MQ=60)
                                                 CGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTG                                             >  SRR4302540.3482465/1‑94 (MQ=60)
                                                 CGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTG                                             <  SRR4302540.4442924/94‑1 (MQ=60)
                                                 CGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAATTTTAACGAAGGGGTG                                             <  SRR4302540.2233298/94‑1 (MQ=60)
                                                  GCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGG                                            <  SRR4302540.2447020/94‑1 (MQ=60)
                                                   CAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGT                                           <  SRR4302540.1596546/94‑1 (MQ=60)
                                                    AGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTT                                          <  SRR4302540.7799941/94‑1 (MQ=60)
                                                    AGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTT                                          >  SRR4302540.9954175/1‑94 (MQ=60)
                                                    AGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTT                                          >  SRR4302540.5154800/1‑94 (MQ=60)
                                                    AGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTT                                          >  SRR4302540.3717557/1‑94 (MQ=60)
                                                     GCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTT                                         <  SRR4302540.7728863/94‑1 (MQ=60)
                                                     GCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTT                                         <  SRR4302540.7728864/94‑1 (MQ=60)
                                                      CTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTC                                        >  SRR4302540.474128/1‑94 (MQ=60)
                                                       TAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCA                                       >  SRR4302540.1904821/1‑94 (MQ=60)
                                                        AACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGGTTCAC                                      >  SRR4302540.6670946/1‑94 (MQ=60)
                                                          CGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCC                                    >  SRR4302540.5303524/1‑94 (MQ=60)
                                                           GTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCC                                   >  SRR4302540.8114882/1‑94 (MQ=60)
                                                           GTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCC                                   >  SRR4302540.1392306/1‑94 (MQ=60)
                                                           GTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCC                                   <  SRR4302540.7928584/94‑1 (MQ=60)
                                                           GTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGGGGTTTCACCCC                                   >  SRR4302540.3480627/1‑94 (MQ=60)
                                                             TCCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTT                                 <  SRR4302540.915734/94‑1 (MQ=60)
                                                             ACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTT                                 >  SRR4302540.6849548/1‑94 (MQ=60)
                                                             ACCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTT                                 <  SRR4302540.9683494/94‑1 (MQ=60)
                                                              CCGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTT                                <  SRR4302540.5597911/94‑1 (MQ=60)
                                                               CGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTT                               <  SRR4302540.6276000/94‑1 (MQ=60)
                                                               CGGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTT                               <  SRR4302540.6806687/94‑1 (MQ=60)
                                                                GGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTG                              <  SRR4302540.9138192/94‑1 (MQ=60)
                                                                GGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTG                              <  SRR4302540.2333417/94‑1 (MQ=60)
                                                                GGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTG                              >  SRR4302540.9824656/1‑94 (MQ=60)
                                                                GGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTG                              >  SRR4302540.10128237/1‑94 (MQ=60)
                                                                CGCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTG                              <  SRR4302540.10111791/94‑1 (MQ=60)
                                                                 GCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGT                             <  SRR4302540.4634302/94‑1 (MQ=60)
                                                                 GCATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGT                             <  SRR4302540.3600597/94‑1 (MQ=60)
                                                                  CATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTCAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTC                            >  SRR4302540.4612900/1‑94 (MQ=60)
                                                                   ATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAtgagcgatttcccccattttgtat                           >  SRR4302540.3563806/1‑70 (MQ=60)
                                                                   ATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGTGGTGGTTTCACCCCTTTTGTCT                           >  SRR4302540.8799980/1‑94 (MQ=60)
                                                                   ATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCT                           >  SRR4302540.8826936/1‑94 (MQ=60)
                                                                   ATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCT                           >  SRR4302540.3563804/1‑94 (MQ=60)
                                                                   ATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCT                           >  SRR4302540.6387411/1‑94 (MQ=60)
                                                                   ATTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGGGGTTTaccccctttttttt                           >  SRR4302540.8661730/1‑80 (MQ=60)
                                                                    TTTGTTTCTGGGAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTT                          <  SRR4302540.3005981/94‑1 (MQ=60)
                                                                    TTTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTT                          >  SRR4302540.6155916/1‑94 (MQ=60)
                                                                    TTGGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTT                          <  SRR4302540.3005986/94‑1 (MQ=60)
                                                                     TTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTT                         <  SRR4302540.7577665/94‑1 (MQ=60)
                                                                     TTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTT                         <  SRR4302540.7577666/94‑1 (MQ=60)
                                                                     TTGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTT                         <  SRR4302540.9960041/94‑1 (MQ=60)
                                                                      TGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTC                        <  SRR4302540.6484043/94‑1 (MQ=60)
                                                                      TGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTC                        <  SRR4302540.6156201/94‑1 (MQ=60)
                                                                      TGTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTC                        >  SRR4302540.230296/1‑94 (MQ=60)
                                                                       GTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCT                       >  SRR4302540.6381935/1‑94 (MQ=60)
                                                                       GTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCT                       <  SRR4302540.5861982/94‑1 (MQ=60)
                                                                       GTTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCT                       <  SRR4302540.2378863/94‑1 (MQ=60)
                                                                        TTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTG                      <  SRR4302540.2047404/94‑1 (MQ=60)
                                                                        TTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTG                      <  SRR4302540.9697723/94‑1 (MQ=60)
                                                                        TTTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTG                      <  SRR4302540.3966709/94‑1 (MQ=60)
                                                                         TTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGG                     >  SRR4302540.4437098/1‑94 (MQ=60)
                                                                         TTCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGG                     <  SRR4302540.3616472/94‑1 (MQ=60)
                                                                          TCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGC                    <  SRR4302540.1846448/94‑1 (MQ=60)
                                                                          TCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGC                    <  SRR4302540.2715566/94‑1 (MQ=60)
                                                                          TCTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGCTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGC                    >  SRR4302540.9267014/1‑94 (MQ=60)
                                                                           CTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCG                   <  SRR4302540.8348668/94‑1 (MQ=60)
                                                                           CTGGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCG                   <  SRR4302540.668087/94‑1 (MQ=60)
                                                                             GGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTC                 <  SRR4302540.4097153/94‑1 (MQ=60)
                                                                             GGCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTC                 >  SRR4302540.8667257/1‑94 (MQ=60)
                                                                              GCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCG                <  SRR4302540.1289825/94‑1 (MQ=60)
                                                                              GCAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCG                <  SRR4302540.8264148/94‑1 (MQ=60)
                                                                               CAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGA               <  SRR4302540.8773004/94‑1 (MQ=60)
                                                                               CAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGA               <  SRR4302540.5378078/94‑1 (MQ=60)
                                                                               CAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGA               >  SRR4302540.4730054/1‑94 (MQ=60)
                                                                               CAAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTATTTCTGGCGTCGC               >  SRR4302540.1931358/1‑94 (MQ=60)
                                                                                AAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGAT              >  SRR4302540.972190/1‑94 (MQ=60)
                                                                                AAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGAT              >  SRR4302540.8119726/1‑94 (MQ=60)
                                                                                AAGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGAC              >  SRR4302540.3403068/1‑94 (MQ=60)
                                                                                 AGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATC             <  SRR4302540.1662260/94‑1 (MQ=60)
                                                                                 AGGCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATC             >  SRR4302540.9103149/1‑94 (MQ=60)
                                                                                   GCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCAT           <  SRR4302540.1644309/94‑1 (MQ=60)
                                                                                   GCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCAT           <  SRR4302540.6655770/94‑1 (MQ=60)
                                                                                   GCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCAT           <  SRR4302540.5350899/94‑1 (MQ=60)
                                                                                   GCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCAT           <  SRR4302540.7413399/94‑1 (MQ=60)
                                                                                   GCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCAT           <  SRR4302540.3317039/94‑1 (MQ=60)
                                                                                   GCACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCAT           >  SRR4302540.3510780/1‑94 (MQ=60)
                                                                                     ACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTG         <  SRR4302540.6062755/94‑1 (MQ=60)
                                                                                     ACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTG         >  SRR4302540.3331259/1‑94 (MQ=60)
                                                                                     ACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTG         >  SRR4302540.924216/1‑94 (MQ=60)
                                                                                     ACTGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTG         >  SRR4302540.9856954/1‑94 (MQ=60)
                                                                                       TGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGAT       >  SRR4302540.4245529/1‑94 (MQ=60)
                                                                                       TGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGAT       >  SRR4302540.7941282/1‑94 (MQ=60)
                                                                                       TGAAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGAT       <  SRR4302540.4427701/94‑1 (MQ=60)
                                                                                       TGAAAAGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGAT       <  SRR4302540.5417521/94‑1 (MQ=60)
                                                                                         AAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGC     >  SRR4302540.4731167/1‑94 (MQ=60)
                                                                                         AAAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGC     >  SRR4302540.175729/1‑94 (MQ=60)
                                                                                          AAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT    <  SRR4302540.3088402/94‑1 (MQ=60)
                                                                                          AAGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCT    >  SRR4302540.2002599/1‑94 (MQ=60)
                                                                                           AGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTG   >  SRR4302540.2494505/1‑94 (MQ=60)
                                                                                           AGGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTGTCACCCCTTTTGTCTTTCTGGCGTAGATCATTGATGCTG   >  SRR4302540.1095015/1‑94 (MQ=60)
                                                                                            GGCGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGG  >  SRR4302540.3175819/1‑94 (MQ=60)
                                                                                             |                                                                                            
CTGAGCGTACTGGCCGCAACCCGCAGACCGGTAAAGAAATCAAAATTGCCGCAGCTAACGTACCGGCATTTGTTTCTGGCAAGGCACTGAAAGACGCAGTTAAGTAAGATTGCGTGGCAGTGAACAGTTTTAACGAAGGGGTGGTTTCACCCCTTTTGTCTTTCTGGCGTCGATCATTGATGCTGG  >  NC_000913/4198025‑4198210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑