Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,225,139	T→C	intergenic (+49/‑171)	metH → / → yjbB	B12‑dependent homocysteine‑N5‑methyltetrahydrofolate transmethylase/putative alpha helix protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,225,139	0	T	C	100.0%	680.2 / NA	216	intergenic (+49/‑171)	metH/yjbB	B12‑dependent homocysteine‑N5‑methyltetrahydrofolate transmethylase/putative alpha helix protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (117/99); total (117/99)

Only 100 of 322 total aligned reads displayed.

GAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTA  >  NC_000913/4225049‑4225228

gggcgcTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAgcgc                                                                                          <  2:6035865/91‑1 (MQ=255)

gggcgcTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAgcgc                                                                                          >  2:2775574/3‑93 (MQ=255)

     ctggctggCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCATTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGt                                                                                     <  2:9401987/93‑1 (MQ=255)

     ctggctggCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGt                                                                                     >  2:1124376/1‑93 (MQ=255)

     ctggctggCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGt                                                                                     >  1:2791295/1‑93 (MQ=255)

      tggctggcACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGtc                                                                                    <  1:8394042/93‑1 (MQ=255)

      tggctggcACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGtc                                                                                    >  2:9514735/1‑93 (MQ=255)

       ggctggcACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGAGCGCTCAATGAGCGGCCTGtct                                                                                   >  2:808432/1‑93 (MQ=255)

          tggcACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCtt                                                                                >  2:3159234/1‑93 (MQ=255)

           ggcACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCttt                                                                               >  1:1312110/1‑93 (MQ=255)

            gcACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCCATGAGCGCCCTGTCTCtttt                                                                              >  2:5507809/1‑92 (MQ=255)

               ccGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGGGCTCAATCAGCGCCCTGTCTCttttttt                                                                           >  2:7471375/1‑92 (MQ=255)

               ccGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGGGCCCTGTCTCTttatta                                                                           >  2:2621339/1‑93 (MQ=255)

               ccGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTttatta                                                                           >  2:1660123/1‑93 (MQ=255)

                  aaTCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAata                                                                        <  1:6701496/93‑1 (MQ=255)

                    tcgggggTATGACGCGGACTGATTCAAAAATCTGTCTCTTTTCCTTACAAAAAACGGGGCGCTCAAGAAGCTCCCTGTCTCTTTATTAATATg                                                                      <  2:922650/90‑1 (MQ=255)

                    tCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGGGGTCAATGAACCGCCTGTCTCTTTTTTTatata                                                                      >  2:239350/1‑92 (MQ=255)

                      tGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGaa                                                                    <  2:5268866/93‑1 (MQ=255)

                       ggtgTATGACGCGGTCTGTTTCACAATTCTGTCTCTTTCCCTCACAACAAACAGGGCGCTCAAGGAGCCCCCTGTCTCTTTATTAATATGaaa                                                                   <  1:5659370/90‑1 (MQ=255)

                            aTGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACtt                                                              >  1:1679310/1‑93 (MQ=255)

                            aTGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACtt                                                              <  2:3442779/93‑1 (MQ=255)

                            aTGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACtt                                                              >  1:5352939/1‑93 (MQ=255)

                             tGACTCGGACTGATTCAAAAATCTGACGCTTTTCCTTACAAGAAACAGGGCTCTAAATAATGGCCCTGTCTCTTTATTAATATGAAACACTta                                                             <  2:2910904/93‑1 (MQ=255)

                             tGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCGCAATGAGCGCCCTGTTTTTTTATTAATATGAAACACTta                                                             >  1:478684/1‑93 (MQ=255)

                              gACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTta‑t                                                           <  1:1056678/93‑1 (MQ=255)

                              gACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTta‑t                                                           <  1:972364/93‑1 (MQ=255)

                               gcgcgGTGTGATTCACAAACCGATCACTTTCCCTGCCAACAAACGGGGCGCTAAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTta‑ta                                                          <  2:9766341/92‑1 (MQ=255)

                               aCGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTta‑ta                                                          <  1:4635922/93‑1 (MQ=255)

                                 gcgGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACt                                                        <  1:6275271/93‑1 (MQ=255)

                                  cgGACTGATTCACAAATTTGTCTTTTTTCTTAAAAAAAAACGGGGCGCTAAATGGGCCCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTg                                                       <  2:196527/93‑1 (MQ=255)

                                   ggACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGGGCTCAATGAGCGCCCCGTCCCTTTATTTATATTAAACACTTA‑TACTgg                                                      >  1:7401432/1‑93 (MQ=255)

                                   ggACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTgg                                                      >  1:5037276/1‑93 (MQ=255)

                                    gACTGATTCCCAAATCTGTCCCTTTCCCTTACAACAACCAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGa                                                     <  2:8311446/93‑1 (MQ=255)

                                    gACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGa                                                     >  1:9335055/1‑93 (MQ=255)

                                    gACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCCCAATGAGCGCCCCGTCTCTTTTTTTATTTTAAACACTTA‑TTCTTGa                                                     >  2:3895001/1‑93 (MQ=255)

                                      cTGATTCACAATCCTCTCCCTTTCCCTAACAACAAACAGGGCGCTAAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGaaa                                                   <  2:4303493/93‑1 (MQ=255)

                                       tGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAAc                                                  >  2:8210198/1‑93 (MQ=255)

                                       tGATTCACAAATCTGTCACTTTTCCTTAAAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAAc                                                  <  1:2647702/93‑1 (MQ=255)

                                        gATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACa                                                 <  1:8517750/93‑1 (MQ=255)

                                        gATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACa                                                 >  2:8769894/1‑93 (MQ=255)

                                        gATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACa                                                 <  2:7014834/93‑1 (MQ=255)

                                         aTTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGGGCTCAATTAGTGTCCTGTCTCTTTTTTTATGTTAAAAACCTATTCCTGGaaaag                                                 >  2:5539177/1‑91 (MQ=255)

                                          ttCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCCAAGAGCGCCCTGTCTCTTTATTTATATGAAACAATTA‑TACTTGaaaaaag                                               >  1:1920566/1‑89 (MQ=255)

                                          ttCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAgg                                               >  2:4423265/1‑93 (MQ=255)

                                          ttCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAgg                                               >  1:2997445/1‑93 (MQ=255)

                                          ttCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAgg                                               >  2:6430678/1‑93 (MQ=255)

                                            tgaaaaTCTGGCAGTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCt                                             <  2:760514/90‑1 (MQ=255)

                                              caAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCCATTAGCGCCCCGTCTCTTTTTTTATATTAAAAACTTA‑TACTTGAAACAAGCTgg                                           >  2:3805496/1‑93 (MQ=255)

                                                aaTCTGTCATTTTTCCTTACAACAAACAGGGCGCTCAATGAGCCCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGaa                                         <  2:4037608/93‑1 (MQ=255)

                                                aaTCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGaa                                         <  2:7920271/93‑1 (MQ=255)

                                                 agcTGTCACTTTTCCTTACAACAAACAGGGGCCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTCA‑TACTGGAAACAGGCTGGaat                                        <  1:1819844/91‑1 (MQ=255)

                                                  tCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGaata                                       <  1:817536/93‑1 (MQ=255)

                                                    tGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGaataat                                     >  1:758534/1‑93 (MQ=255)

                                                    tGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGaataat                                     <  1:8261956/93‑1 (MQ=255)

                                                      ttatttttCGTTACAATAAAAAGGCCGGTAAATGAGCCCACTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCt                                   <  1:4107185/89‑1 (MQ=255)

                                                       cACTTTTCATTCCAACAAACAGGCCGCGCAGTGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCtt                                  <  1:3896053/93‑1 (MQ=255)

                                                        ctttttCCTCACAACAACCAGGGCGCTAAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTa                                 <  1:4231736/91‑1 (MQ=255)

                                                        cttttcCCTAAAAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTa                                 <  2:473466/91‑1 (MQ=255)

                                                         cTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAg                                <  2:6840303/93‑1 (MQ=255)

                                                          ttttCCTAAAAACAAACGGGCCGCTCAAGGGCCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGc                               <  1:7214827/93‑1 (MQ=255)

                                                           tttCCTTAAAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGcc                              <  1:2695547/93‑1 (MQ=255)

                                                           ttcccTTACAACAACCGGGCCGCTCATTGAGCCCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGcc                              <  1:5912687/90‑1 (MQ=255)

                                                             tctttAAAACAGACAGGGCGCTCAATGAGCGCTCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCgg                            <  1:4110198/90‑1 (MQ=255)

                                                               cTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGaa                          <  1:6513102/93‑1 (MQ=255)

                                                               cTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGaa                          <  2:6109903/93‑1 (MQ=255)

                                                               cTTACAACAAACAGGGCGCTAAAGGGGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGaa                          <  2:7748144/93‑1 (MQ=255)

                                                                ttACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGGATAATCTTAGCCAGaaa                         >  2:9624426/1‑93 (MQ=255)

                                                                ttACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGaaa                         <  1:3539665/93‑1 (MQ=255)

                                                                ttACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGaaa                         >  1:3109185/1‑93 (MQ=255)

                                                                 tACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAAAAGGCTGGAATAATATTAGCCGGaaaa                        >  1:2501900/1‑93 (MQ=255)

                                                                  tcaacaaACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACCCTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGaaaaa                       <  2:8711904/92‑1 (MQ=255)

                                                                  acaacaAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGaaaaa                       >  1:3282257/1‑93 (MQ=255)

                                                                  acaacaAACAGGGCGCTAAATGAGCTCACTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGaaaaa                       <  2:3547043/93‑1 (MQ=255)

                                                                   cagaaaacaGGGCACAAAATGAGCGCGCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAAc                      <  1:465545/89‑1 (MQ=255)

                                                                   aaaaaaacgGGCCGCTAAATGAGCTCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAAc                      <  2:2239325/89‑1 (MQ=255)

                                                                    aacaaacaGGGCCCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACg                     <  2:9819593/93‑1 (MQ=255)

                                                                      caaacaGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGaa                   <  1:9501069/93‑1 (MQ=255)

                                                                      aaaacaGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGaa                   <  1:9201915/92‑1 (MQ=255)

                                                                       aaacaGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAAt                  >  2:1734777/1‑93 (MQ=255)

                                                                        accggggCGCTAAAGGAGCCCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATg                 <  1:9138682/89‑1 (MQ=255)

                                                                        aacaGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATg                 <  1:3619839/93‑1 (MQ=255)

                                                                        aacaGGGCGCTAAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATg                 <  1:8951935/93‑1 (MQ=255)

                                                                            gggCGTTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCaa             <  2:1245914/93‑1 (MQ=255)

                                                                             ggCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCCGGAAAACGAATGTCaaa            >  2:902363/1‑93 (MQ=255)

                                                                             ggCGCTAAATGAGCCCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCaaa            <  2:5791045/93‑1 (MQ=255)

                                                                               cgcTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAg          >  1:5181847/1‑93 (MQ=255)

                                                                                gcTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAgg         >  2:5917641/1‑93 (MQ=255)

                                                                                 cTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGc        >  2:1397268/1‑93 (MQ=255)

                                                                                 cTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGc        >  2:5384890/1‑93 (MQ=255)

                                                                                 cTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGc        >  2:9303107/1‑93 (MQ=255)

                                                                                    aaTGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGcaca     <  2:656275/93‑1 (MQ=255)

                                                                                    aaTGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGcaca     <  1:6879845/93‑1 (MQ=255)

                                                                                    aaTGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGcaca     >  1:2392214/1‑93 (MQ=255)

                                                                                    aaTGAGCGCCCTGTCTCTTTATTAATATGAAACACCTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGcaca     >  2:8475043/1‑93 (MQ=255)

                                                                                    aaTGAGCCCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGcaca     <  2:5186114/93‑1 (MQ=255)

                                                                                     aTGAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAg    <  2:6706356/93‑1 (MQ=255)

                                                                                      ggAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGTAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGt   <  1:8662380/92‑1 (MQ=255)

                                                                                       gAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTa  >  1:9898891/1‑93 (MQ=255)

                                                                                       gAGCGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTa  >  1:4352915/1‑93 (MQ=255)

                                                                                       gAGCGCCCTGCCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTa  <  1:3403024/93‑1 (MQ=255)

GAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTA‑TACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTA  >  NC_000913/4225049‑4225228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGA  >  NC_000913/4225046‑4225232

GTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGG                                                                                               >  SRR4302540.9011477/1‑94 (MQ=60)

GTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGC                                                                                               <  SRR4302540.2675662/94‑1 (MQ=60)

GTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGC                                                                                               >  SRR4302540.3278953/1‑94 (MQ=60)

GTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCCCTCAATGAGC                                                                                               <  SRR4302540.2675663/94‑1 (MQ=60)

   GGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCC                                                                                            <  SRR4302540.4152417/94‑1 (MQ=60)

     GCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCT                                                                                          <  SRR4302540.4333832/94‑1 (MQ=60)

     GCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCT                                                                                          <  SRR4302540.9391979/94‑1 (MQ=60)

       GCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGT                                                                                        >  SRR4302540.5199028/1‑94 (MQ=60)

       GCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGT                                                                                        >  SRR4302540.2821434/1‑94 (MQ=60)

        CTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGAGCCCCGTC                                                                                       >  SRR4302540.3888906/1‑94 (MQ=60)

         TGGCTGGCCCCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCT                                                                                      <  SRR4302540.2948113/94‑1 (MQ=60)

         TGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCT                                                                                      <  SRR4302540.8491451/94‑1 (MQ=60)

          GGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTC                                                                                     <  SRR4302540.8291245/94‑1 (MQ=60)

          GGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTC                                                                                     <  SRR4302540.3522685/94‑1 (MQ=60)

            CTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTT                                                                                   <  SRR4302540.5312784/94‑1 (MQ=60)

             TGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTT                                                                                  >  SRR4302540.1325745/1‑94 (MQ=60)

             TGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGCGCGCTCAATGAGCGCCCTGTCTCTTT                                                                                  >  SRR4302540.3896939/1‑94 (MQ=60)

              GGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTA                                                                                 >  SRR4302540.9888950/1‑94 (MQ=60)

               GCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTAT                                                                                <  SRR4302540.9493171/94‑1 (MQ=60)

                CACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTGTCTTTATT                                                                               >  SRR4302540.9803972/1‑94 (MQ=60)

                 ACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTA                                                                              <  SRR4302540.8302737/94‑1 (MQ=60)

                    GAATCTGGGTTATGACGCGGACTGATTCACAAATCTGTCTCTTTTCTTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATA                                                                           <  SRR4302540.9561559/94‑1 (MQ=60)

                    GAATCGGGGGTAGGACGCGGACTGATTCACAAATCTGTCCCTTTTCCTTACAACAAACAGGGCGCTGAATGAGCGCCCTGTCTCTTTATTAATA                                                                           <  SRR4302540.2659198/94‑1 (MQ=60)

                     AATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATAT                                                                          <  SRR4302540.6775470/94‑1 (MQ=60)

                      ATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATG                                                                         >  SRR4302540.360200/1‑94 (MQ=60)

                      ATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATG                                                                         <  SRR4302540.1257114/94‑1 (MQ=60)

                        CTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAA                                                                       >  SRR4302540.5733700/1‑94 (MQ=60)

                         tggggtatacccagaattaATTCAAAAATCCGTCATTTTTCCTTACAAAAAACAGGCCGCTCAATGAGCCCCCTGTCTCTTTATTAATATGAAA                                                                      <  SRR4302540.7655845/75‑1 (MQ=41)

                          GGTGTATGACGCGGTCTGTTTCACAATTCTGTCTCTTTCCCTCACAACAAACAGGGCGCTCAAGGAGCCCCCTGTCTCTTTATTAATATGAAAC                                                                     <  SRR4302540.5721686/94‑1 (MQ=46)

                          GGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAAC                                                                     <  SRR4302540.2695467/94‑1 (MQ=60)

                          GGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAAC                                                                     <  SRR4302540.3420653/94‑1 (MQ=60)

                             GTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACT                                                                  >  SRR4302540.6883882/1‑94 (MQ=60)

                              TATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTTATATGAAACACTT                                                                 >  SRR4302540.502515/1‑94 (MQ=60)

                              TATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTT                                                                 >  SRR4302540.1697537/1‑94 (MQ=60)

                              TATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTT                                                                 >  SRR4302540.5411708/1‑94 (MQ=60)

                              TATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTT                                                                 >  SRR4302540.4742824/1‑94 (MQ=60)

                              TATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTT                                                                 >  SRR4302540.1714188/1‑94 (MQ=60)

                               ATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCGCAATGAGCGCCCTGTTTTTTTATTAATATGAAACACTTA                                                                >  SRR4302540.483812/1‑94 (MQ=60)

                                TGCCGCGTACTGATTCAAAAATCTGTCTCTTTCCCTTACAACAAACGGGCCCCTCAAGGACCCCCCTGTCTCTTTATTAATATGAAACACTTAT                                                               <  SRR4302540.25394/94‑1 (MQ=26)

                                TGCCGCGGACTAATTAAAAAATCTGTCTTTTTTCTTTACAACAAAAAGGGCCCTCAAGGGGCCCCCTGTCTCTTTATTAATATGAAACACTTAT                                                               <  SRR4302540.253988/94‑1 (MQ=18)

                                TGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCCATGAGCGCCCTTTCTCTTTTTTAATATTAAAAACTTAT                                                               >  SRR4302540.7492699/1‑94 (MQ=60)

                                TGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTAT                                                               >  SRR4302540.6401106/1‑94 (MQ=60)

                                TGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCCGTCTCTTTTTTTATATTAAAAACTTAT                                                               >  SRR4302540.7241930/1‑94 (MQ=60)

                                 GACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATA                                                              <  SRR4302540.1067432/94‑1 (MQ=60)

                                 GACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATA                                                              <  SRR4302540.982362/94‑1 (MQ=60)

                                  ACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATAC                                                             <  SRR4302540.8618031/94‑1 (MQ=60)

                                  ACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATAC                                                             <  SRR4302540.4685559/94‑1 (MQ=60)

                                   CGCGGACTGATTCACAAATCTTTCATTTTTCCTAACAACAAACAGGGCGCTAAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACT                                                            <  SRR4302540.243510/94‑1 (MQ=60)

                                   CGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGCGCGCTCCATGAAAGGCCCGTCTCCTTATTTATATGAAACACTTATAAT                                                            >  SRR4302540.4153811/1‑94 (MQ=60)

                                    GCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTTATATGAAACACTTATACTG                                                           >  SRR4302540.9166993/1‑94 (MQ=60)

                                    GCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTG                                                           <  SRR4302540.6345082/94‑1 (MQ=60)

                                    GCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTG                                                           <  SRR4302540.3075191/94‑1 (MQ=60)

                                     CGGACTGATTCACAAATCTGTCACTTTTCCTTACATCAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGG                                                          >  SRR4302540.2943066/1‑94 (MQ=60)

                                     CGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGGGCTCAATGAGCGCCCCGTCCCTTTATTTATATTAAACACTTATACTGG                                                          >  SRR4302540.7483890/1‑94 (MQ=60)

                                     CGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGG                                                          <  SRR4302540.8309852/94‑1 (MQ=60)

                                     CGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGG                                                          >  SRR4302540.2411204/1‑94 (MQ=60)

                                     CGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGG                                                          >  SRR4302540.7950742/1‑94 (MQ=60)

                                     CGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGG                                                          >  SRR4302540.5091767/1‑94 (MQ=60)

                                      GGACTGATTCACAAATCTGTCTCTTTTCCTAACAAAAAACAGGGCGCTAAATGAGCCCCCTGTCTCTTTATTAATATGAAACACTTATACTGGA                                                         <  SRR4302540.7334559/94‑1 (MQ=60)

                                      GGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGA                                                         >  SRR4302540.8881947/1‑94 (MQ=60)

                                      GGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGA                                                         >  SRR4302540.9446339/1‑94 (MQ=60)

                                       GACTGATTCACAAATCTGTCATTTTTCCTTACAACAAACGGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAA                                                        <  SRR4302540.2493016/94‑1 (MQ=60)

                                       GACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAA                                                        <  SRR4302540.6969760/94‑1 (MQ=60)

                                         CTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAAC                                                      <  SRR4302540.9629917/94‑1 (MQ=60)

                                         CTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAAC                                                      >  SRR4302540.3073463/1‑94 (MQ=60)

                                         CTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAAC                                                      <  SRR4302540.942026/94‑1 (MQ=60)

                                          TGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACA                                                     >  SRR4302540.6517406/1‑94 (MQ=60)

                                          TGATTCACAAATCTGTCACTTTTCCTTAAAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACA                                                     <  SRR4302540.2676422/94‑1 (MQ=60)

                                           GATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAG                                                    <  SRR4302540.8616738/94‑1 (MQ=60)

                                            ATTCACAAATCTGTCACTTTTCCTTACAACAAACAGTGGGCTCAAAGAGCGCCCCGGCTCTCTATTAATATGAAAaaactatactagaaacaag                                                   >  SRR4302540.1941516/1‑75 (MQ=41)

                                            ATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCCAAGAGCGCCCTGTCTCTTTATTTATATGAAACAATTATACTtgaaaaaag                                                   >  SRR4302540.1941517/1‑85 (MQ=60)

                                            ATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGG                                                   >  SRR4302540.3030475/1‑94 (MQ=60)

                                             TTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGC                                                  >  SRR4302540.1007286/1‑94 (MQ=60)

                                             TTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGC                                                  >  SRR4302540.1842211/1‑94 (MQ=60)

                                             TTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGC                                                  >  SRR4302540.501588/1‑94 (MQ=60)

                                             TTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGC                                                  >  SRR4302540.4749273/1‑94 (MQ=60)

                                             TTCACAAATCTGTCACTTTTCCTTACAACAAACAGAGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAAAAGGC                                                  >  SRR4302540.217343/1‑94 (MQ=60)

                                              TCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAAAACTTATACTGGAAACAGGCT                                                 >  SRR4302540.4177400/1‑94 (MQ=60)

                                               CACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGGGCAATGAGGGCGCTGTCTCTTTTTTTATATGAAAAACTTATtattgaaaaaagctg                                                >  SRR4302540.5643190/1‑79 (MQ=49)

                                               CACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTG                                                >  SRR4302540.3527046/1‑94 (MQ=60)

                                                ACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGG                                               <  SRR4302540.6589110/94‑1 (MQ=60)

                                                ACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGG                                               <  SRR4302540.8602057/94‑1 (MQ=60)

                                                   AATCTGTCCTTTTCCCTGACAACAAACAGGGCACTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAAT                                            <  SRR4302540.9560917/94‑1 (MQ=60)

                                                   AATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAAT                                            >  SRR4302540.2803048/1‑94 (MQ=60)

                                                   AATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAAT                                            <  SRR4302540.1661229/94‑1 (MQ=60)

                                                    ATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGACCCCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATA                                           <  SRR4302540.8677583/94‑1 (MQ=60)

                                                    AGCTGTCACTTTTCCTTACAACAAACAGGGGCCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTCATACTGGAAACAGGCTGGAATA                                           <  SRR4302540.1839727/94‑1 (MQ=60)

                                                     TCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAA                                          <  SRR4302540.826225/94‑1 (MQ=60)

                                                     TCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCCGTCTCTTTTTTTATATTAAAAACTTTTACTGGGAAAAGGCTGGGATAA                                          >  SRR4302540.5600214/1‑94 (MQ=42)

                                                      CTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAAT                                         >  SRR4302540.766606/1‑94 (MQ=60)

                                                       TGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATC                                        <  SRR4302540.8357964/94‑1 (MQ=60)

                                                       TGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATC                                        >  SRR4302540.6926520/1‑94 (MQ=60)

                                                        GTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCT                                       >  SRR4302540.9994612/1‑94 (MQ=60)

                                                         TCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTT                                      <  SRR4302540.6645672/94‑1 (MQ=60)

                                                         TCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTT                                      <  SRR4302540.1739486/94‑1 (MQ=60)

                                                          CACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTA                                     >  SRR4302540.4411015/1‑94 (MQ=60)

                                                          CACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTA                                     <  SRR4302540.2924756/94‑1 (MQ=60)

                                                          CACTTTTCATTCCAACAAACAGGCCGCGCAGTGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTA                                     <  SRR4302540.3938336/94‑1 (MQ=60)

                                                           ctTTTTCCTCACAACAACCAGGGCGCTAAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAG                                    <  SRR4302540.4277796/92‑1 (MQ=60)

                                                            CTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGC                                   >  SRR4302540.5799681/1‑94 (MQ=60)

                                                             TTTTCCTAACAAAAAACAGGCCGCTCATGGAGCCCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCC                                  <  SRR4302540.9013676/94‑1 (MQ=60)

                                                              TTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCG                                 >  SRR4302540.3636350/1‑94 (MQ=60)

                                                              TTTCCTTAAAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCG                                 <  SRR4302540.2724648/94‑1 (MQ=60)

                                                              TTCCCTTACAACAACCGGGCCGCTCATTGAGCCCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCG                                 <  SRR4302540.5978009/94‑1 (MQ=60)

                                                               TTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG                                >  SRR4302540.8600562/1‑94 (MQ=60)

                                                                TCTTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGA                               <  SRR4302540.8392136/94‑1 (MQ=60)

                                                                TCTTTAAAACAGACAGGGCGCTCAATGAGCGCTCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGA                               <  SRR4302540.4154564/94‑1 (MQ=60)

                                                                TCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGA                               <  SRR4302540.4154565/94‑1 (MQ=60)

                                                                TCCTCACAAAAAACAGCGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGA                               <  SRR4302540.4154567/94‑1 (MQ=60)

                                                                 CCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAA                              >  SRR4302540.3353916/1‑94 (MQ=60)

                                                                  CTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAA                             <  SRR4302540.6585455/94‑1 (MQ=60)

                                                                  CTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAA                             >  SRR4302540.3143207/1‑94 (MQ=60)

                                                                   taaaAACAAGCAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAA                            <  SRR4302540.1948979/90‑1 (MQ=60)

                                                                   TTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAA                            <  SRR4302540.3578323/94‑1 (MQ=60)

                                                                   TTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAA                            >  SRR4302540.3236816/1‑94 (MQ=60)

                                                                   TTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCCGAAAA                            >  SRR4302540.4543753/1‑94 (MQ=60)

                                                                   TTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAAAAGGCTGGAATAATATTAGCCGGAAAA                            >  SRR4302540.2528898/1‑94 (MQ=60)

                                                                   TTACAACAAACAGGGCGCTCAATGAGCCCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAA                            <  SRR4302540.768771/94‑1 (MQ=60)

                                                                    TACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAA                           >  SRR4302540.3318131/1‑94 (MQ=60)

                                                                    TACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAA                           <  SRR4302540.3521166/94‑1 (MQ=60)

                                                                     ACAACAAACAGGGCGCTAAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAAC                          <  SRR4302540.4371240/94‑1 (MQ=60)

                                                                      CAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACA                         >  SRR4302540.5387576/1‑94 (MQ=60)

                                                                         CAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAAT                      <  SRR4302540.9613650/94‑1 (MQ=60)

                                                                         CAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAAT                      <  SRR4302540.6303362/94‑1 (MQ=60)

                                                                         AAAACAGGGCTCTAATTGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAAT                      <  SRR4302540.8477024/94‑1 (MQ=60)

                                                                         AAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAAT                      <  SRR4302540.9312322/94‑1 (MQ=60)

                                                                          AAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATG                     <  SRR4302540.4752958/94‑1 (MQ=60)

                                                                          AAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATG                     >  SRR4302540.1469187/1‑94 (MQ=60)

                                                                           AACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGT                    <  SRR4302540.3659441/94‑1 (MQ=60)

                                                                           AACAGGGCGCTAAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGT                    <  SRR4302540.9057492/94‑1 (MQ=60)

                                                                               GGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAA                >  SRR4302540.9836541/1‑94 (MQ=60)

                                                                               GGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAA                >  SRR4302540.4692623/1‑94 (MQ=60)

                                                                                GGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAA               <  SRR4302540.5215765/94‑1 (MQ=60)

                                                                                 GCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAG              <  SRR4302540.5793824/94‑1 (MQ=60)

                                                                                 GCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAG              >  SRR4302540.5238255/1‑94 (MQ=60)

                                                                                  CGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGG             >  SRR4302540.5589630/1‑94 (MQ=60)

                                                                                   GCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGC            >  SRR4302540.7603860/1‑94 (MQ=60)

                                                                                    CTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCA           >  SRR4302540.9264417/1‑94 (MQ=60)

                                                                                      CAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACA         <  SRR4302540.6015030/94‑1 (MQ=60)

                                                                                      CAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACA         >  SRR4302540.2417990/1‑94 (MQ=60)

                                                                                      CAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACA         <  SRR4302540.2762453/94‑1 (MQ=60)

                                                                                      CAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACA         >  SRR4302540.6798956/1‑94 (MQ=60)

                                                                                      AAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACA         <  SRR4302540.267803/94‑1 (MQ=60)

                                                                                       AATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAG        <  SRR4302540.3176437/94‑1 (MQ=60)

                                                                                       AATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAG        <  SRR4302540.6956104/94‑1 (MQ=60)

                                                                                       AATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAAACATCTTAGCCGGAAAAACGAATGTGAAAAGACCCAG        >  SRR4302540.2626677/1‑94 (MQ=60)

                                                                                        ATGAGCGCCCTGTCTCTTGATTAATATGAAACACTTATGCTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGT       <  SRR4302540.4696910/94‑1 (MQ=60)

                                                                                         TGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTA      >  SRR4302540.10014524/1‑94 (MQ=60)

                                                                                         TGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTA      >  SRR4302540.4400265/1‑94 (MQ=60)

                                                                                         TGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTA      <  SRR4302540.889445/94‑1 (MQ=60)

                                                                                          GAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAA     >  SRR4302540.627955/1‑94 (MQ=60)

                                                                                          GAGCGCCCTGCCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAA     <  SRR4302540.3440123/94‑1 (MQ=60)

                                                                                           AGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAA    >  SRR4302540.8933307/1‑94 (MQ=60)

                                                                                            GGGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAG   <  SRR4302540.4615491/94‑1 (MQ=60)

                                                                                            GCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAG   <  SRR4302540.3569158/94‑1 (MQ=60)

                                                                                             CGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGA  <  SRR4302540.2585408/94‑1 (MQ=60)

GTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGA  >  NC_000913/4225046‑4225232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`