Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,244,543 T→C F61L (TTC→CTC)  malK → ATP‑binding component of transport system for maltose

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,244,5430TC99.7% 1208.4 / NA 374F61L (TTC→CTC) malKATP‑binding component of transport system for maltose
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (169/204);  minor base G (0/1);  total (169/205)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 392 total aligned reads displayed.

AATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTG  >  NC_000913/4244451‑4244632
                                                                                            |                                                                                         
   tCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCa                                                                                        >  2:8723242/1‑93 (MQ=255)
   tCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCa                                                                                        <  2:2338552/93‑1 (MQ=255)
    cGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCAt                                                                                       <  1:9404387/93‑1 (MQ=255)
    cGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCAt                                                                                       <  1:8687540/93‑1 (MQ=255)
       ggtgTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCgg                                                                                    <  2:8656160/93‑1 (MQ=255)
          ttttGTCGGACAGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTga                                                                                 <  2:7094871/92‑1 (MQ=255)
          gtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTga                                                                                 >  2:6674845/1‑93 (MQ=255)
           tttGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTgag                                                                                >  1:3857433/1‑93 (MQ=255)
           tttGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTgag                                                                                >  2:6237977/1‑93 (MQ=255)
             tGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGaa                                                                              >  2:8257202/1‑93 (MQ=255)
              gTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGaaa                                                                             <  1:9517979/93‑1 (MQ=255)
               tCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAAc                                                                            <  2:642003/93‑1 (MQ=255)
                cGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACg                                                                           <  1:6924462/93‑1 (MQ=255)
                cGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACg                                                                           <  1:7899420/93‑1 (MQ=255)
                 ggACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACgg                                                                          >  2:844532/1‑93 (MQ=255)
                   aCCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATAACCAGCGGCGACCTGCTCATCGGTGAGAAACGGat                                                                        <  1:1806028/93‑1 (MQ=255)
                     cGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatga                                                                      >  2:7822093/1‑93 (MQ=255)
                     cGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatga                                                                      >  1:119941/1‑93 (MQ=255)
                      gTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatgaa                                                                     <  2:6034460/93‑1 (MQ=255)
                       tCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatgaat                                                                    <  2:6762120/93‑1 (MQ=255)
                        cTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatgaatg                                                                   >  2:474969/1‑93 (MQ=255)
                        cTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatgaatg                                                                   <  1:6565791/93‑1 (MQ=255)
                         tGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatgaatga                                                                  <  1:2428090/93‑1 (MQ=255)
                         tGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatgaatga                                                                  >  2:1569369/1‑93 (MQ=255)
                           gCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGaca                                                                >  1:3332174/1‑93 (MQ=255)
                           gCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGaca                                                                <  1:5609206/93‑1 (MQ=255)
                           gCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGaca                                                                <  1:4999042/93‑1 (MQ=255)
                           gCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGaca                                                                <  2:2119588/93‑1 (MQ=255)
                           gCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGaca                                                                >  2:4608427/1‑93 (MQ=255)
                           gCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGaca                                                                <  1:1000136/93‑1 (MQ=255)
                            cTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGacac                                                               <  2:3351372/93‑1 (MQ=255)
                            cTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGacac                                                               <  2:5118723/93‑1 (MQ=255)
                            cTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGacac                                                               >  2:9769254/1‑93 (MQ=255)
                              gCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTc                                                             <  2:2271325/93‑1 (MQ=255)
                              gCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTc                                                             <  1:1764151/93‑1 (MQ=255)
                               cGGTAAATCGACTTTGCTGCGCGTGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGCAACGGATGCATGACACTcc                                                            <  1:8967262/93‑1 (MQ=38)
                               cGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTcc                                                            <  1:2729839/93‑1 (MQ=255)
                               cGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTcc                                                            <  1:3488969/93‑1 (MQ=255)
                                 gTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGc                                                          >  2:9518342/1‑93 (MQ=255)
                                 gTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGc                                                          >  2:4076276/1‑93 (MQ=255)
                                    aaTCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCcag                                                       >  2:1039703/1‑93 (MQ=255)
                                    aaTCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCcag                                                       >  1:6750870/1‑93 (MQ=255)
                                     agcGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCcagc                                                      <  1:3721409/91‑1 (MQ=255)
                                       cGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCcagcag                                                    >  1:5093530/1‑93 (MQ=255)
                                        gACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGa                                                   >  1:9962003/1‑93 (MQ=255)
                                         aCTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGaa                                                  <  1:9904045/93‑1 (MQ=255)
                                         aCTTTACTGACCATGAGTGCGGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGaa                                                  <  1:6264723/93‑1 (MQ=255)
                                          cTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAc                                                 <  1:2993018/93‑1 (MQ=255)
                                           tttACTGCGCATGATTGCCTGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGaagg                                                >  2:1996360/1‑91 (MQ=255)
                                            ttACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAcgc                                               <  2:2848915/93‑1 (MQ=255)
                                            ttACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAcgc                                               <  1:6630169/93‑1 (MQ=255)
                                              aCTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACgcgg                                             <  2:8561948/93‑1 (MQ=255)
                                                tGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACgcggcg                                           >  1:3662530/1‑93 (MQ=255)
                                                tGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACgcggcg                                           >  1:7287115/1‑93 (MQ=255)
                                                 gcgcATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGt                                          <  1:565475/93‑1 (MQ=255)
                                                   gcATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTg                                        >  1:1985915/1‑93 (MQ=255)
                                                   gcATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTg                                        >  2:7406583/1‑93 (MQ=255)
                                                   gcATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTg                                        <  1:6124311/93‑1 (MQ=255)
                                                    cATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTgg                                       <  2:4871658/93‑1 (MQ=255)
                                                    cATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTgg                                       >  1:9109646/1‑93 (MQ=255)
                                                     aTGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGt                                      >  2:1325688/1‑93 (MQ=255)
                                                      tGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGCGAAACGGATGAATGACACTCCGCCAGCAGAACGCCGCGTTGGTa                                     >  1:6854067/1‑93 (MQ=255)
                                                      tGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTa                                     <  2:3097167/93‑1 (MQ=255)
                                                       gATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGATACTCCGCCAGCAGAACGCGCCGTTGGTAt                                    >  2:9420626/1‑93 (MQ=255)
                                                       gATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTAt                                    >  1:7626054/1‑93 (MQ=255)
                                                          tGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGgt                                 <  2:2604219/93‑1 (MQ=255)
                                                          tGCCGGGCTTGAGACAATCCCCAGCGGCGTCCTGCTCATCGGTGAGAAACGAATGAATGACGCTCCGCTGGCAGAACGCGGCGTTGGTAAGgt                                 <  2:5681181/93‑1 (MQ=255)
                                                           tccGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGgtg                                <  1:7505873/92‑1 (MQ=255)
                                                             cGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGtt                              <  2:4458208/93‑1 (MQ=255)
                                                             cGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGtt                              >  1:7725197/1‑93 (MQ=255)
                                                              gggCTTGAGACGATCACCAGCGGCGACCTGCTCCTCGGTGAGAAACGGATGAATGACACTCGCCCAGCAAAACGGGGCGTGGGATTGGGGttt                             >  1:2837294/1‑93 (MQ=255)
                                                               ggCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTc                            <  1:1289360/93‑1 (MQ=255)
                                                               ggCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTc                            <  2:6634259/93‑1 (MQ=255)
                                                               ggCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTc                            >  1:8092805/1‑93 (MQ=255)
                                                               ggCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACAATCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTc                            >  1:5455133/1‑93 (MQ=255)
                                                                gCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCa                           <  1:4571537/93‑1 (MQ=255)
                                                                gCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCa                           <  1:8591551/93‑1 (MQ=255)
                                                                gCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCa                           <  1:4005445/93‑1 (MQ=255)
                                                                gCTTGAGACGATCACCAGCGGCAACCTGCTCATCGGTGAGAAACGGATTAATGACCCTCCGCCAGCAGAACGCGGCGTTGGTATGGTGtttta                           <  2:4348917/93‑3 (MQ=255)
                                                                 cTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAg                          <  1:4268504/93‑1 (MQ=255)
                                                                  ttGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGt                         <  2:5929239/93‑1 (MQ=255)
                                                                     agaCGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCCGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCtt                      >  1:3209917/1‑93 (MQ=255)
                                                                      gccGATCACCAGCGGCGCCCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACTCGGCGTTGGTATGGTGTTTCAGTCTTa                     <  1:5785246/91‑1 (MQ=255)
                                                                       aCGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAc                    <  1:1037849/93‑1 (MQ=255)
                                                                          aTCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAcgcg                 >  2:7764082/1‑93 (MQ=255)
                                                                          aTCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAcgcg                 >  1:2553054/1‑93 (MQ=255)
                                                                            cACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGct               >  2:7185094/1‑93 (MQ=255)
                                                                               cAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTa            >  2:9681082/1‑93 (MQ=255)
                                                                               cAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTa            <  1:637693/93‑1 (MQ=255)
                                                                                aGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTAt           <  2:3211717/93‑1 (MQ=255)
                                                                                 gcggcgACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATc          <  1:2317794/93‑1 (MQ=255)
                                                                                    gcgACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATcccc       <  1:1615346/93‑1 (MQ=255)
                                                                                      gACCTGCTCATCGGTGAGAAACGGATGAATGACGCTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCAc     <  2:3896970/93‑1 (MQ=255)
                                                                                      gACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCAc     >  2:5435229/1‑93 (MQ=255)
                                                                                       aCCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCAcc    >  1:1389475/1‑93 (MQ=255)
                                                                                        ccTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCt   <  2:7348177/93‑1 (MQ=255)
                                                                                        ccTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCt   >  1:472307/1‑93 (MQ=255)
                                                                                         cTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTg  <  2:8047556/93‑1 (MQ=255)
                                                                                         cTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTg  >  2:8944757/1‑93 (MQ=255)
                                                                                         cTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTg  <  1:7003199/93‑1 (MQ=255)
                                                                                            |                                                                                         
AATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTG  >  NC_000913/4244451‑4244632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 221 total aligned reads displayed.
AATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAG  >  NC_000913/4244451‑4244636
                                                                                            |                                                                                             
AATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCT                                                                                              >  SRR4302540.7561948/1‑94 (MQ=60)
 ATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTC                                                                                             <  SRR4302540.6791738/94‑1 (MQ=60)
 ATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTC                                                                                             <  SRR4302540.4638869/94‑1 (MQ=60)
   TCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCAT                                                                                           >  SRR4302540.6774316/1‑94 (MQ=60)
    CGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATC                                                                                          <  SRR4302540.4023553/94‑1 (MQ=60)
    CGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATC                                                                                          <  SRR4302540.9516248/94‑1 (MQ=60)
    CGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATC                                                                                          >  SRR4302540.6649835/1‑94 (MQ=60)
    CGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATC                                                                                          <  SRR4302540.5411328/94‑1 (MQ=60)
    CGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATC                                                                                          >  SRR4302540.10075335/1‑94 (MQ=60)
    CGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATC                                                                                          <  SRR4302540.8788413/94‑1 (MQ=60)
     GTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCG                                                                                         <  SRR4302540.5391637/94‑1 (MQ=60)
     GTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCG                                                                                         <  SRR4302540.4195122/94‑1 (MQ=60)
      TGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGG                                                                                        <  SRR4302540.5635460/94‑1 (MQ=60)
      TGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGG                                                                                        >  SRR4302540.6721573/1‑94 (MQ=60)
      TGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGG                                                                                        >  SRR4302540.6925320/1‑94 (MQ=60)
       GGTGTTTGTCGGACCATCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGT                                                                                       <  SRR4302540.3775251/94‑1 (MQ=60)
        GTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTG                                                                                      >  SRR4302540.1031939/1‑94 (MQ=60)
        GTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTG                                                                                      <  SRR4302540.5932661/94‑1 (MQ=60)
        GTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTG                                                                                      >  SRR4302540.7225740/1‑94 (MQ=60)
          GTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAG                                                                                    >  SRR4302540.3899371/1‑94 (MQ=60)
          GTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAG                                                                                    >  SRR4302540.1737282/1‑94 (MQ=60)
              GTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAAC                                                                                >  SRR4302540.5064290/1‑94 (MQ=60)
              GTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAAC                                                                                <  SRR4302540.6690318/94‑1 (MQ=60)
              GTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAAC                                                                                <  SRR4302540.9630699/94‑1 (MQ=60)
                ccgcCCGTCTGGCTGCGGTAAATCGGCTTTACCGCGCATGGTTGCCGGGCTTCAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGG                                                                              <  SRR4302540.3425209/90‑1 (MQ=60)
                CGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGG                                                                              <  SRR4302540.7991565/94‑1 (MQ=60)
                CGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGG                                                                              <  SRR4302540.7001521/94‑1 (MQ=60)
                CGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGG                                                                              <  SRR4302540.3425213/94‑1 (MQ=60)
                  GACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGAT                                                                            <  SRR4302540.1166264/94‑1 (MQ=60)
                  GACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGAT                                                                            <  SRR4302540.4632026/94‑1 (MQ=60)
                   ACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATAACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATG                                                                           <  SRR4302540.1825701/94‑1 (MQ=60)
                    CCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGA                                                                          >  SRR4302540.573942/1‑94 (MQ=60)
                    CCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGA                                                                          >  SRR4302540.121232/1‑94 (MQ=60)
                      GTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAAT                                                                        >  SRR4302540.10115296/1‑94 (MQ=60)
                      GTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAAT                                                                        <  SRR4302540.7942752/94‑1 (MQ=60)
                      GTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAAT                                                                        <  SRR4302540.4708847/94‑1 (MQ=60)
                      GTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAAT                                                                        <  SRR4302540.3632909/94‑1 (MQ=60)
                       TCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATG                                                                       <  SRR4302540.10090953/94‑1 (MQ=60)
                        CTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGA                                                                      >  SRR4302540.7779196/1‑94 (MQ=60)
                        CTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGA                                                                      >  SRR4302540.7779195/1‑94 (MQ=60)
                        CTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGA                                                                      >  SRR4302540.3755350/1‑94 (MQ=60)
                        CTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGA                                                                      <  SRR4302540.6638556/94‑1 (MQ=60)
                         TGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGAC                                                                     <  SRR4302540.2454249/94‑1 (MQ=60)
                         TGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGAC                                                                     <  SRR4302540.4254800/94‑1 (MQ=60)
                         TGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGAC                                                                     <  SRR4302540.5031247/94‑1 (MQ=60)
                         TGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGAC                                                                     <  SRR4302540.8155166/94‑1 (MQ=60)
                          TGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGATACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACA                                                                    <  SRR4302540.1652568/94‑1 (MQ=60)
                          GGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACA                                                                    >  SRR4302540.8799352/1‑94 (MQ=60)
                          GGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACA                                                                    >  SRR4302540.3368762/1‑94 (MQ=60)
                          GGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACA                                                                    <  SRR4302540.3903902/94‑1 (MQ=60)
                           GCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACAC                                                                   <  SRR4302540.5670914/94‑1 (MQ=60)
                           GCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACAC                                                                   <  SRR4302540.2950670/94‑1 (MQ=60)
                           GCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACAC                                                                   <  SRR4302540.1010295/94‑1 (MQ=60)
                           GCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACAC                                                                   <  SRR4302540.5053169/94‑1 (MQ=60)
                           GCTGCGGTAAATCGACTTGACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACAC                                                                   <  SRR4302540.1747762/94‑1 (MQ=60)
                           GCGGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACAC                                                                   <  SRR4302540.2950669/94‑1 (MQ=60)
                             TGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTC                                                                 >  SRR4302540.9585776/1‑94 (MQ=60)
                              acccctcttccgatctagcgcttATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCC                                                                <  SRR4302540.2361939/71‑1 (MQ=60)
                              GCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGCCCTGCTCATCGGTGAGAAACGGATGAATGACACTCC                                                                <  SRR4302540.1783574/94‑1 (MQ=60)
                              GCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCC                                                                <  SRR4302540.5772008/94‑1 (MQ=60)
                              GCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCC                                                                <  SRR4302540.1783286/94‑1 (MQ=60)
                               CGGTAAATCGACTTTGCTGCGCGTGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGCAACGGATGCATGACACTCCG                                                               <  SRR4302540.9073580/94‑1 (MQ=60)
                               CGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCG                                                               <  SRR4302540.2759291/94‑1 (MQ=60)
                               CGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCG                                                               <  SRR4302540.3526965/94‑1 (MQ=60)
                                  TAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCA                                                            >  SRR4302540.1913460/1‑94 (MQ=60)
                                   AAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCGGCCAG                                                           >  SRR4302540.790789/1‑94 (MQ=60)
                                   AAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAG                                                           <  SRR4302540.5693548/94‑1 (MQ=60)
                                   AAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAG                                                           >  SRR4302540.7846215/1‑94 (MQ=60)
                                   AAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAG                                                           >  SRR4302540.6825462/1‑94 (MQ=60)
                                    AATCGACTTTACTGCGCATGATTGCCGGGCTTTAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGC                                                          >  SRR4302540.1013242/1‑94 (MQ=60)
                                     atcgcgttttCTGCGCATGATTGCCTGTCTGGAGACGACCACCAGGGTCGACCTGCCGATCGGTGAGAAACGGATGAATGACACTCCGCCAGCA                                                         <  SRR4302540.9459217/84‑1 (MQ=35)
                                     AGCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCA                                                         <  SRR4302540.3762009/94‑1 (MQ=60)
                                      TCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAG                                                        >  SRR4302540.5148638/1‑94 (MQ=60)
                                      TCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAG                                                        >  SRR4302540.3109483/1‑94 (MQ=60)
                                       CGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGA                                                       >  SRR4302540.3622445/1‑94 (MQ=60)
                                       CGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGA                                                       >  SRR4302540.10078242/1‑94 (MQ=60)
                                       CGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGA                                                       >  SRR4302540.6304524/1‑94 (MQ=60)
                                       CGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGA                                                       >  SRR4302540.8170918/1‑94 (MQ=60)
                                         acttttctgcgcagtatgtCCGGGCTTGAGCCGATCACCAGTGGCGACCTGGTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAC                                                     <  SRR4302540.2498044/75‑1 (MQ=60)
                                         ACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAC                                                     <  SRR4302540.10019756/94‑1 (MQ=60)
                                         ACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAC                                                     <  SRR4302540.6496778/94‑1 (MQ=60)
                                         ACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAC                                                     <  SRR4302540.2498043/94‑1 (MQ=60)
                                          CTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACG                                                    <  SRR4302540.706015/94‑1 (MQ=60)
                                          CTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACG                                                    >  SRR4302540.645897/1‑94 (MQ=60)
                                          CTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACG                                                    <  SRR4302540.3026013/94‑1 (MQ=60)
                                          CTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACG                                                    >  SRR4302540.4775183/1‑94 (MQ=60)
                                          CTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACG                                                    >  SRR4302540.9335547/1‑94 (MQ=60)
                                           TTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGC                                                   >  SRR4302540.6834299/1‑94 (MQ=60)
                                            TTTCTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCCGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCG                                                  <  SRR4302540.2642106/94‑1 (MQ=60)
                                            TTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCG                                                  >  SRR4302540.2105524/1‑94 (MQ=60)
                                            TTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCG                                                  <  SRR4302540.1497929/94‑1 (MQ=60)
                                            TTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCG                                                  <  SRR4302540.3476679/94‑1 (MQ=60)
                                            TTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCG                                                  <  SRR4302540.6703563/94‑1 (MQ=60)
                                            TTACGGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCG                                                  <  SRR4302540.2218083/94‑1 (MQ=60)
                                             TACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGG                                                 <  SRR4302540.353016/94‑1 (MQ=60)
                                              ACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGC                                                <  SRR4302540.7546881/94‑1 (MQ=60)
                                               CTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCG                                               >  SRR4302540.3702618/1‑94 (MQ=60)
                                               CTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCG                                               >  SRR4302540.7368428/1‑94 (MQ=60)
                                                TGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGT                                              >  SRR4302540.795781/1‑94 (MQ=60)
                                                 TCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTT                                             <  SRR4302540.2856254/94‑1 (MQ=60)
                                                 GCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTT                                             <  SRR4302540.571624/94‑1 (MQ=60)
                                                  CGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAACCGGATGAATGACCCTCCGCCAGCAGAACGCGGCGTTG                                            <  SRR4302540.1497026/94‑1 (MQ=60)
                                                  CGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTG                                            >  SRR4302540.2007446/1‑94 (MQ=60)
                                                   GCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAAGGGATGAATGACACTCCGCCAGCAGAACGCGGCGTGGG                                           >  SRR4302540.9219121/1‑94 (MQ=60)
                                                   GCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGG                                           <  SRR4302540.6192520/94‑1 (MQ=60)
                                                   GCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGG                                           >  SRR4302540.9219122/1‑94 (MQ=60)
                                                     ATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGCGAAACGGATGAATGACACTCCGCCAGCAGAACGCCGCGTTGGTA                                         >  SRR4302540.6929994/1‑94 (MQ=60)
                                                     ATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCATAACGCGGCGTTGGTA                                         <  SRR4302540.1263349/94‑1 (MQ=60)
                                                     ATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTA                                         <  SRR4302540.6171020/94‑1 (MQ=60)
                                                     ATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTA                                         >  SRR4302540.3756667/1‑94 (MQ=60)
                                                     ATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTA                                         >  SRR4302540.6929995/1‑94 (MQ=60)
                                                      TGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTAT                                        >  SRR4302540.7711816/1‑94 (MQ=60)
                                                       GATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATG                                       <  SRR4302540.2912710/94‑1 (MQ=60)
                                                       GATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATG                                       >  SRR4302540.1819853/1‑94 (MQ=60)
                                                        ATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGG                                      >  SRR4302540.1200989/1‑94 (MQ=60)
                                                        ATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGG                                      >  SRR4302540.6820386/1‑94 (MQ=60)
                                                        ATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGG                                      >  SRR4302540.2510730/1‑94 (MQ=60)
                                                        ATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGG                                      >  SRR4302540.8616404/1‑94 (MQ=60)
                                                        ATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGG                                      <  SRR4302540.5627478/94‑1 (MQ=60)
                                                         TTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGT                                     <  SRR4302540.1165143/94‑1 (MQ=60)
                                                         TTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGT                                     <  SRR4302540.2004733/94‑1 (MQ=60)
                                                           TCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGT                                   <  SRR4302540.7589766/94‑1 (MQ=60)
                                                           GCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGT                                   >  SRR4302540.7112882/1‑94 (MQ=60)
                                                            CCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTT                                  >  SRR4302540.7815321/1‑94 (MQ=60)
                                                            CCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTT                                  <  SRR4302540.5426238/94‑1 (MQ=60)
                                                             CGGGCTTGAGACGATCACCAGCGTCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTT                                 <  SRR4302540.9106687/94‑1 (MQ=60)
                                                             CGGGCTTGAGACGATCACCAGCGGCGACCTGCTCCTCGGTGAGAAACGGATGAATGACACTCgcccagcaaaacggggcgtgggattggggttt                                 >  SRR4302540.2867985/1‑62 (MQ=60)
                                                             CGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTT                                 <  SRR4302540.7781742/94‑1 (MQ=60)
                                                              GGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTC                                >  SRR4302540.8186745/1‑94 (MQ=60)
                                                              GGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTC                                >  SRR4302540.8801849/1‑94 (MQ=60)
                                                               GGCTTGAGACGGTCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCA                               <  SRR4302540.7373924/94‑1 (MQ=60)
                                                               GGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCA                               <  SRR4302540.5694700/94‑1 (MQ=60)
                                                               GGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCA                               <  SRR4302540.1302680/94‑1 (MQ=60)
                                                               GGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCA                               <  SRR4302540.1302679/94‑1 (MQ=60)
                                                               GGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCA                               >  SRR4302540.3353165/1‑94 (MQ=60)
                                                               GGCGTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCA                               <  SRR4302540.4266991/94‑1 (MQ=60)
                                                                GCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAG                              <  SRR4302540.4048573/94‑1 (MQ=60)
                                                                GCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAG                              <  SRR4302540.756427/94‑1 (MQ=60)
                                                                GCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAG                              <  SRR4302540.8691428/94‑1 (MQ=60)
                                                                GCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAG                              <  SRR4302540.4620879/94‑1 (MQ=60)
                                                                 CTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGT                             <  SRR4302540.121856/94‑1 (MQ=60)
                                                                 CTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGT                             >  SRR4302540.5688941/1‑94 (MQ=60)
                                                                  TTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTC                            >  SRR4302540.9466917/1‑94 (MQ=60)
                                                                  TTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTC                            >  SRR4302540.7046874/1‑94 (MQ=60)
                                                                   TGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCT                           <  SRR4302540.1321348/94‑1 (MQ=60)
                                                                    GAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCCGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTT                          >  SRR4302540.3244766/1‑94 (MQ=60)
                                                                    GAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTT                          >  SRR4302540.445103/1‑94 (MQ=60)
                                                                    GAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTT                          >  SRR4302540.3244767/1‑94 (MQ=60)
                                                                    GAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTT                          >  SRR4302540.769228/1‑94 (MQ=60)
                                                                     AGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTA                         <  SRR4302540.9751076/94‑1 (MQ=60)
                                                                     AGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTA                         <  SRR4302540.5597120/94‑1 (MQ=60)
                                                                      GCCGATCACCAGCGGCGCCCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACTCGGCGTTGGTATGGTGTTTCAGTCTTAC                        <  SRR4302540.5849155/94‑1 (MQ=60)
                                                                      GACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAC                        >  SRR4302540.3123514/1‑94 (MQ=60)
                                                                      GACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAC                        <  SRR4302540.8495923/94‑1 (MQ=60)
                                                                      GACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAC                        <  SRR4302540.281171/94‑1 (MQ=60)
                                                                      GACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAC                        >  SRR4302540.6436143/1‑94 (MQ=60)
                                                                      GACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAC                        >  SRR4302540.6436144/1‑94 (MQ=60)
                                                                       aggaccgccgGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACG                       <  SRR4302540.5019905/84‑1 (MQ=60)
                                                                        CGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGC                      <  SRR4302540.326674/94‑1 (MQ=60)
                                                                        CGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGC                      <  SRR4302540.3781723/94‑1 (MQ=60)
                                                                        CGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGC                      >  SRR4302540.8705858/1‑94 (MQ=60)
                                                                        CGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGC                      >  SRR4302540.4717005/1‑94 (MQ=60)
                                                                        CGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGC                      <  SRR4302540.9552996/94‑1 (MQ=60)
                                                                         GATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCG                     >  SRR4302540.6959206/1‑94 (MQ=60)
                                                                         GATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCG                     >  SRR4302540.2580569/1‑94 (MQ=60)
                                                                         GATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCG                     >  SRR4302540.2580568/1‑94 (MQ=60)
                                                                         GATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCG                     >  SRR4302540.6280267/1‑94 (MQ=60)
                                                                         CATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGCCCCTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCG                     <  SRR4302540.7080754/94‑1 (MQ=60)
                                                                           TCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTGCGCCAGCAGAACGCGGCGGTGGTATGGTGTTTCAGTCTTACGCGCT                   >  SRR4302540.9180273/1‑94 (MQ=60)
                                                                           TCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCT                   >  SRR4302540.6278064/1‑94 (MQ=60)
                                                                            CACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTC                  >  SRR4302540.6415935/1‑94 (MQ=60)
                                                                            CACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTC                  <  SRR4302540.1211758/94‑1 (MQ=60)
                                                                            CACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTC                  >  SRR4302540.4588362/1‑94 (MQ=60)
                                                                            CACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTC                  <  SRR4302540.8289710/94‑1 (MQ=60)
                                                                             ACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCT                 >  SRR4302540.76559/1‑94 (MQ=60)
                                                                             ACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCA                 >  SRR4302540.8862556/1‑94 (MQ=60)
                                                                               CAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTAT               <  SRR4302540.644477/94‑1 (MQ=60)
                                                                               CAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTAT               <  SRR4302540.644478/94‑1 (MQ=60)
                                                                                CGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATC              <  SRR4302540.2384140/94‑1 (MQ=60)
                                                                                 GCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCC             <  SRR4302540.2342748/94‑1 (MQ=60)
                                                                                 GCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCC             <  SRR4302540.402641/94‑1 (MQ=60)
                                                                                  CGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACCCTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCC            <  SRR4302540.4566013/94‑1 (MQ=60)
                                                                                  CGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCC            >  SRR4302540.5095679/1‑94 (MQ=60)
                                                                                   GGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCC           >  SRR4302540.10039446/1‑94 (MQ=60)
                                                                                    GCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCA          <  SRR4302540.1633066/94‑1 (MQ=60)
                                                                                    GCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCA          >  SRR4302540.9668537/1‑94 (MQ=60)
                                                                                     CGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCAC         <  SRR4302540.8726041/94‑1 (MQ=60)
                                                                                      GACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACC        <  SRR4302540.5941313/94‑1 (MQ=60)
                                                                                      GACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACC        >  SRR4302540.3730004/1‑94 (MQ=60)
                                                                                      GACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACC        >  SRR4302540.2173473/1‑94 (MQ=60)
                                                                                      GACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACC        <  SRR4302540.1944128/94‑1 (MQ=60)
                                                                                      GACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACC        >  SRR4302540.1404343/1‑94 (MQ=60)
                                                                                      GACCTGCTCAGCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACC        <  SRR4302540.10041135/94‑1 (MQ=60)
                                                                                       ACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCT       >  SRR4302540.477366/1‑94 (MQ=60)
                                                                                         CTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGT     >  SRR4302540.7290140/1‑94 (MQ=60)
                                                                                         CTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGT     <  SRR4302540.7080905/94‑1 (MQ=60)
                                                                                         CTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGT     >  SRR4302540.978686/1‑94 (MQ=60)
                                                                                          TGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTC    >  SRR4302540.3255696/1‑94 (MQ=60)
                                                                                           GCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGGATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCA   >  SRR4302540.6649774/1‑94 (MQ=60)
                                                                                            CTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGATCTATCCCCACCTGTCAG  >  SRR4302540.6220936/1‑94 (MQ=60)
                                                                                            |                                                                                             
AATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAG  >  NC_000913/4244451‑4244636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑