Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,244,543 T→C F61L (TTC→CTC)  malK → ATP‑binding component of transport system for maltose

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,244,5430TC100.0% 744.6 / NA 229F61L (TTC→CTC) malKATP‑binding component of transport system for maltose
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (101/128);  total (101/128)

Only 100 of 231 total aligned reads displayed.

TTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTG  >  NC_000913/4244453‑4244632
                                                                                          |                                                                                         
ttCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTc                                                                                         >  2:1326296/1‑93 (MQ=255)
 tCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCa                                                                                        <  2:5964617/93‑1 (MQ=255)
  cGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCAt                                                                                       <  1:4030280/93‑1 (MQ=255)
   gtggtgTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATc                                                                                      <  1:1598443/93‑1 (MQ=255)
    tggtgTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCg                                                                                     >  2:6154152/1‑93 (MQ=255)
    tggtgTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCg                                                                                     <  2:1208201/93‑1 (MQ=255)
    tggtgTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCg                                                                                     <  2:4201764/93‑1 (MQ=255)
        gtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTga                                                                                 >  2:4679699/1‑93 (MQ=255)
        gtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTga                                                                                 <  2:4265764/93‑1 (MQ=255)
         tttGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTgag                                                                                >  1:3733277/1‑93 (MQ=255)
         tttGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTgag                                                                                <  2:4103895/93‑1 (MQ=255)
          ttGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTgaga                                                                               <  2:4438643/93‑1 (MQ=255)
           tGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGaa                                                                              <  1:2524235/93‑1 (MQ=255)
             tCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAAc                                                                            >  2:165777/1‑93 (MQ=255)
               ggcccGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGAGTGCCGGGCTTGAGAAGATCACCAGCGGCGCCCTGCTCATCCATGAGAAACgg                                                                          >  2:2137512/4‑93 (MQ=37)
               ggACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACgg                                                                          >  1:1183272/1‑93 (MQ=255)
               ggACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACgg                                                                          >  1:3984815/1‑93 (MQ=255)
                 aCCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGat                                                                        <  1:2471815/93‑1 (MQ=255)
                  ccGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatg                                                                       <  1:1330178/93‑1 (MQ=255)
                   cGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatga                                                                      <  1:3203323/93‑1 (MQ=255)
                     tCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatgaat                                                                    >  1:3912185/1‑93 (MQ=255)
                      cTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatgaatg                                                                   <  2:134458/93‑1 (MQ=255)
                       tGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatgaatgc                                                                  >  1:2587410/1‑92 (MQ=255)
                       tGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatgaatga                                                                  >  2:2815218/1‑93 (MQ=255)
                        ggCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGac                                                                 >  1:2150499/1‑93 (MQ=255)
                        ggCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGac                                                                 >  2:5808567/1‑93 (MQ=255)
                        ggCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGac                                                                 <  2:4773921/93‑1 (MQ=255)
                         gCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGaca                                                                <  1:625592/93‑1 (MQ=255)
                         gCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGaca                                                                >  1:4525011/1‑93 (MQ=255)
                           tGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACt                                                              >  2:1859948/1‑93 (MQ=255)
                            gCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTc                                                             <  2:1664501/93‑1 (MQ=255)
                            gCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTc                                                             <  2:3815548/93‑1 (MQ=255)
                            gCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTc                                                             <  1:6069908/93‑1 (MQ=255)
                              ggTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCg                                                           >  1:3669982/1‑93 (MQ=255)
                               gTAACTCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGc                                                          <  1:2970544/93‑1 (MQ=255)
                               gTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGc                                                          <  2:891703/93‑1 (MQ=255)
                                tAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGcc                                                         <  2:2446825/93‑1 (MQ=255)
                                   aTCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCcagc                                                      >  1:1000062/1‑93 (MQ=255)
                                    tCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCcagca                                                     <  1:1275523/93‑1 (MQ=255)
                                    tCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCcagca                                                     <  2:3046942/93‑1 (MQ=255)
                                       aCTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGaa                                                  <  2:2659772/93‑1 (MQ=255)
                                       aCTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGaa                                                  >  2:1549489/1‑93 (MQ=255)
                                       aCTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGaa                                                  <  1:981492/93‑1 (MQ=255)
                                        cTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAc                                                 <  1:1893433/93‑1 (MQ=255)
                                        cTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAc                                                 >  2:3519787/1‑93 (MQ=255)
                                         tttACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAcg                                                >  1:2862989/1‑93 (MQ=255)
                                         tttACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAcg                                                >  2:1295353/1‑93 (MQ=255)
                                          ttACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAcgc                                               <  1:4388097/93‑1 (MQ=255)
                                          ttACTGCGCATGATTGCCGGGCTTGAGACGAGCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAcgc                                               <  2:3189696/93‑1 (MQ=255)
                                           tACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGAAACTCGGACAGCAGAAcgcg                                              >  2:3097659/1‑93 (MQ=37)
                                            aCTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACgcgg                                             <  2:2904932/93‑1 (MQ=255)
                                            aCTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACgcgg                                             >  1:3775354/1‑93 (MQ=255)
                                             cTGCGCATGATTGCCGGGCTTGAGACGATCGCCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACgcggc                                            <  2:5913338/93‑1 (MQ=255)
                                             cTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACgcggc                                            <  2:3033778/93‑1 (MQ=255)
                                              tGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACgcggcg                                           <  1:1132276/93‑1 (MQ=255)
                                              tGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACgcggcg                                           <  1:4712163/93‑1 (MQ=255)
                                              tGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACgcggcg                                           >  1:2734477/1‑93 (MQ=255)
                                                    tGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTa                                     >  2:2397948/1‑93 (MQ=255)
                                                    tGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTa                                     <  2:2763121/93‑1 (MQ=255)
                                                     gATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTAt                                    >  1:3246957/1‑93 (MQ=255)
                                                       ttGCCGGGCTTGATACGATCACCATCGGCGACCTACTCATCGGTGAGAAACGGATGAATGACACACCGCCAGCAGAACGCGGCGTTGGTATgg                                  <  2:4148328/93‑1 (MQ=255)
                                                       ttGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATgg                                  <  2:1642475/93‑1 (MQ=255)
                                                        tGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGgt                                 <  2:3606200/93‑1 (MQ=255)
                                                          ccGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGgtgt                               >  1:5049916/1‑93 (MQ=255)
                                                            gggCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGTGGCGTTGGTATGGTGttt                             >  1:1971368/1‑93 (MQ=255)
                                                             ggCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTc                            <  2:5306330/93‑1 (MQ=255)
                                                             ggCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTc                            >  2:2801442/1‑93 (MQ=255)
                                                             ggCTTGAGACGATCACCAGCGGCGACCTGCTCATCCGTGAGAAACGGATGAATGACAATCCGCCAGCAGAACGCTGCGATGGTATGGTGTTTc                            >  2:2263851/1‑93 (MQ=255)
                                                              gCTTGAGACTATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCa                           <  2:5405693/93‑1 (MQ=255)
                                                              gCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCa                           >  1:591362/1‑93 (MQ=255)
                                                               cTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAg                          >  1:2062463/1‑93 (MQ=255)
                                                               cTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAg                          <  1:2236572/93‑1 (MQ=255)
                                                                ttGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGt                         <  2:755490/93‑1 (MQ=255)
                                                                ttGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGt                         <  2:5485346/93‑1 (MQ=255)
                                                                ttGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGt                         >  1:5577932/1‑93 (MQ=255)
                                                                ttGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGt                         >  2:366876/1‑93 (MQ=255)
                                                                ttGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGt                         >  2:5129513/1‑93 (MQ=255)
                                                                ttGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGt                         >  1:1627736/1‑93 (MQ=255)
                                                                 tGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTc                        <  2:1599905/93‑1 (MQ=255)
                                                                    gaCGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTa                     <  1:1039718/93‑1 (MQ=255)
                                                                    gaCGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTa                     <  1:5389176/93‑1 (MQ=255)
                                                                     aCGATCGCCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACGCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAc                    <  1:2208839/93‑1 (MQ=255)
                                                                     aCGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAc                    <  1:5149149/93‑1 (MQ=255)
                                                                      cGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAcg                   <  1:4424934/93‑1 (MQ=255)
                                                                      cGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAcg                   <  2:4559878/93‑1 (MQ=255)
                                                                      cGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAcg                   <  1:3902821/93‑1 (MQ=255)
                                                                      cGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAcc                   >  1:447977/1‑92 (MQ=255)
                                                                          cACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGct               <  2:3649010/93‑1 (MQ=255)
                                                                           cccAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGctc              <  1:1693978/92‑1 (MQ=255)
                                                                           aCCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGctc              <  1:1286806/93‑1 (MQ=255)
                                                                           aCCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGctc              <  1:498994/93‑1 (MQ=255)
                                                                             cAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTa            >  1:3323054/1‑93 (MQ=255)
                                                                              aGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTAt           <  2:397422/93‑1 (MQ=255)
                                                                               gcggcgACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATc          <  2:520080/93‑1 (MQ=255)
                                                                                    gACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCAc     >  1:2027624/1‑93 (MQ=255)
                                                                                    gACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCAc     <  2:3517580/93‑1 (MQ=255)
                                                                                     aCCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCAcc    <  2:4484131/93‑1 (MQ=255)
                                                                                     aCCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCAcc    <  1:5334662/93‑1 (MQ=255)
                                                                                      ccTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCt   <  1:4602430/93‑1 (MQ=255)
                                                                                       cTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTg  >  1:3482097/1‑93 (MQ=255)
                                                                                          |                                                                                         
TTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTG  >  NC_000913/4244453‑4244632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAG  >  NC_000913/4244450‑4244636
                                                                                             |                                                                                             
GAATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGC                                                                                               <  SRR4302533.4469312/94‑1 (MQ=60)
 AATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCT                                                                                              <  SRR4302533.4640390/94‑1 (MQ=60)
 AATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCT                                                                                              >  SRR4302533.4912073/1‑94 (MQ=60)
 AATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCT                                                                                              <  SRR4302533.5609693/94‑1 (MQ=60)
  ATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTC                                                                                             <  SRR4302533.2304784/94‑1 (MQ=60)
   TTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCA                                                                                            <  SRR4302533.5177909/94‑1 (MQ=60)
   TTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCA                                                                                            <  SRR4302533.1147133/94‑1 (MQ=60)
    TCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCAT                                                                                           <  SRR4302533.1082845/94‑1 (MQ=60)
     CGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATC                                                                                          <  SRR4302533.4054811/94‑1 (MQ=60)
      GTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCG                                                                                         >  SRR4302533.4267342/1‑94 (MQ=60)
      GTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCG                                                                                         >  SRR4302533.1893988/1‑94 (MQ=60)
      GTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCG                                                                                         <  SRR4302533.3764605/94‑1 (MQ=60)
      GTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCG                                                                                         <  SRR4302533.1608120/94‑1 (MQ=60)
       TGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGG                                                                                        <  SRR4302533.3824994/94‑1 (MQ=60)
           GTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAG                                                                                    >  SRR4302533.2514206/1‑94 (MQ=60)
           GTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAG                                                                                    >  SRR4302533.3756179/1‑94 (MQ=60)
              TGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAA                                                                                 <  SRR4302533.2539783/94‑1 (MQ=60)
              TGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAA                                                                                 <  SRR4302533.4714814/94‑1 (MQ=60)
               GTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAAC                                                                                <  SRR4302533.2051827/94‑1 (MQ=60)
               GTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAAC                                                                                >  SRR4302533.3841399/1‑94 (MQ=60)
               GTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAAA                                                                                >  SRR4302533.6087142/1‑94 (MQ=60)
                 CGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGG                                                                              >  SRR4302533.4009077/1‑94 (MQ=60)
                 CGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGG                                                                              <  SRR4302533.1494740/94‑1 (MQ=60)
                 CGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGG                                                                              >  SRR4302533.3722136/1‑94 (MQ=60)
                 CGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGG                                                                              >  SRR4302533.1190378/1‑94 (MQ=60)
                  GGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACCGA                                                                             >  SRR4302533.2827175/1‑94 (MQ=60)
                   GACCGTCTGGCCGCGGTAAATCGACTTTCCTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGAT                                                                            <  SRR4302533.4623276/94‑1 (MQ=60)
                    ACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATG                                                                           <  SRR4302533.2487059/94‑1 (MQ=60)
                    ACCGTCTGGCTGATGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTTAGACGATCCCCAGCGGCGACCTGCTCATCGGTGAGAAACGGATG                                                                           <  SRR4302533.921807/94‑1 (MQ=60)
                     CCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGA                                                                          >  SRR4302533.3194212/1‑94 (MQ=60)
                     CCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGA                                                                          <  SRR4302533.1338159/94‑1 (MQ=60)
                      CGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAA                                                                         <  SRR4302533.3223038/94‑1 (MQ=60)
                      CGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAA                                                                         >  SRR4302533.1299948/1‑94 (MQ=60)
                       GTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAAT                                                                        >  SRR4302533.3936075/1‑94 (MQ=60)
                        TCTGGCTGCGGTAAATCCACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATG                                                                       <  SRR4302533.1827861/94‑1 (MQ=60)
                         CTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGC                                                                      >  SRR4302533.2603317/1‑94 (MQ=60)
                         CTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGA                                                                      >  SRR4302533.4030962/1‑94 (MQ=60)
                         CTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGA                                                                      <  SRR4302533.2614515/94‑1 (MQ=60)
                          TGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGAC                                                                     <  SRR4302533.3255872/94‑1 (MQ=60)
                          TGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGAC                                                                     >  SRR4302533.5108310/1‑94 (MQ=60)
                          TGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGAC                                                                     >  SRR4302533.2163741/1‑94 (MQ=60)
                          TGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGAC                                                                     >  SRR4302533.21549/1‑94 (MQ=60)
                           GGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACA                                                                    >  SRR4302533.4552407/1‑94 (MQ=60)
                            GCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACAC                                                                   <  SRR4302533.629266/94‑1 (MQ=60)
                             CTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACT                                                                  <  SRR4302533.1390899/94‑1 (MQ=60)
                               GCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCC                                                                <  SRR4302533.6106634/94‑1 (MQ=60)
                               GCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCC                                                                <  SRR4302533.6230467/94‑1 (MQ=60)
                                CGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCG                                                               >  SRR4302533.3692542/1‑94 (MQ=60)
                                CGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCG                                                               >  SRR4302533.3290730/1‑94 (MQ=60)
                                 GGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGC                                                              >  SRR4302533.4021547/1‑94 (MQ=60)
                                  GTAACTCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCC                                                             <  SRR4302533.2988708/94‑1 (MQ=60)
                                   TAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCA                                                            >  SRR4302533.2618075/1‑94 (MQ=60)
                                    AAATCGACTTTACTGCGCATGATTGCCCGGCTTCAGCCGCTGACCAGCGGGGACCTGCTCATCGGTGAGTAACGGtagactgacactacaccac                                                           >  SRR4302533.5641951/1‑75 (MQ=30)
                                     AATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGC                                                          >  SRR4302533.1017118/1‑94 (MQ=60)
                                     AATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGC                                                          >  SRR4302533.3473173/1‑94 (MQ=60)
                                     AATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGC                                                          >  SRR4302533.1005899/1‑94 (MQ=60)
                                     AATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGC                                                          >  SRR4302533.1289010/1‑94 (MQ=60)
                                      ATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCA                                                         >  SRR4302533.2903015/1‑94 (MQ=60)
                                       TCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAG                                                        <  SRR4302533.1283193/94‑1 (MQ=60)
                                         GACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAA                                                      >  SRR4302533.5385856/1‑94 (MQ=60)
                                         GACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAA                                                      >  SRR4302533.1955066/1‑94 (MQ=60)
                                         GACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAA                                                      >  SRR4302533.2361590/1‑94 (MQ=60)
                                          ACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAC                                                     <  SRR4302533.987245/94‑1 (MQ=60)
                                           CTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACG                                                    <  SRR4302533.1904993/94‑1 (MQ=60)
                                           CTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACG                                                    >  SRR4302533.2880599/1‑94 (MQ=60)
                                            TTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGC                                                   >  SRR4302533.4669941/1‑94 (MQ=60)
                                             TTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCG                                                  <  SRR4302533.4414589/94‑1 (MQ=60)
                                              TACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGG                                                 >  SRR4302533.3798497/1‑94 (MQ=60)
                                               ACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGC                                                <  SRR4302533.4451525/94‑1 (MQ=60)
                                                CTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCG                                               >  SRR4302533.5321147/1‑94 (MQ=60)
                                                CTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCG                                               >  SRR4302533.2751249/1‑94 (MQ=60)
                                                 TGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGT                                              <  SRR4302533.4740723/94‑1 (MQ=60)
                                                 TGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGT                                              <  SRR4302533.1139008/94‑1 (MQ=60)
                                                  GCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTT                                             >  SRR4302533.2408648/1‑94 (MQ=60)
                                                   CGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTG                                            >  SRR4302533.5459507/1‑94 (MQ=60)
                                                   CGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTG                                            >  SRR4302533.5789161/1‑94 (MQ=60)
                                                       TGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTAT                                        >  SRR4302533.3266979/1‑94 (MQ=60)
                                                       TGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTAT                                        >  SRR4302533.1990861/1‑94 (MQ=60)
                                                        GATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATG                                       <  SRR4302533.1663165/94‑1 (MQ=60)
                                                         ATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGG                                      <  SRR4302533.3182043/94‑1 (MQ=60)
                                                         ATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGG                                      <  SRR4302533.1225211/94‑1 (MQ=60)
                                                          TTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGT                                     <  SRR4302533.4506568/94‑1 (MQ=60)
                                                            GCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGT                                   >  SRR4302533.5080460/1‑94 (MQ=60)
                                                             CCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTT                                  >  SRR4302533.5664714/1‑94 (MQ=60)
                                                              CGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGTGGCGTTGGTATGGTGTTT                                 >  SRR4302533.1983431/1‑94 (MQ=60)
                                                              CGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTT                                 >  SRR4302533.392903/1‑94 (MQ=60)
                                                              CGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTT                                 >  SRR4302533.2050379/1‑94 (MQ=60)
                                                                GGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCA                               >  SRR4302533.594854/1‑94 (MQ=60)
                                                                 GCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAG                              <  SRR4302533.2216809/94‑1 (MQ=60)
                                                                 GCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAG                              >  SRR4302533.2075181/1‑94 (MQ=60)
                                                                  CTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGT                             >  SRR4302533.1630918/1‑94 (MQ=60)
                                                                  CTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGT                             <  SRR4302533.2250399/94‑1 (MQ=60)
                                                                  CTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGT                             >  SRR4302533.5611642/1‑94 (MQ=60)
                                                                  CTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGT                             >  SRR4302533.1637595/1‑94 (MQ=60)
                                                                   TTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTC                            <  SRR4302533.938850/94‑1 (MQ=60)
                                                                    TGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCT                           <  SRR4302533.3652671/94‑1 (MQ=60)
                                                                       GACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAC                        <  SRR4302533.3474401/94‑1 (MQ=60)
                                                                       GACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAC                        <  SRR4302533.5421561/94‑1 (MQ=60)
                                                                       GACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAC                        >  SRR4302533.4883746/1‑94 (MQ=60)
                                                                       GACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAC                        <  SRR4302533.1045829/94‑1 (MQ=60)
                                                                        ACGATCGCCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACGCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACG                       <  SRR4302533.2222462/94‑1 (MQ=60)
                                                                        ACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACG                       <  SRR4302533.1221960/94‑1 (MQ=60)
                                                                        ACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACG                       <  SRR4302533.5180259/94‑1 (MQ=60)
                                                                        ACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACC                       >  SRR4302533.450654/1‑94 (MQ=60)
                                                                         CGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGC                      <  SRR4302533.3926657/94‑1 (MQ=60)
                                                                         CGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGC                      <  SRR4302533.5035160/94‑1 (MQ=60)
                                                                         CGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGC                      <  SRR4302533.4451682/94‑1 (MQ=60)
                                                                          GATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCG                     <  SRR4302533.4476849/94‑1 (MQ=60)
                                                                          GATCACCAGCGGCGACCTGCTCATCGGCGAGAAACGGATGAATGACACTCCGCCAGCAGAACTCGGCGTTCGTATGGTGTTTCAGTCTTACGGG                     >  SRR4302533.192876/1‑94 (MQ=60)
                                                                             CACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTC                  >  SRR4302533.2226121/1‑94 (MQ=60)
                                                                              CCCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCT                 <  SRR4302533.1704240/94‑1 (MQ=60)
                                                                              ACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCT                 <  SRR4302533.501964/94‑1 (MQ=60)
                                                                              ACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCT                 <  SRR4302533.1294547/94‑1 (MQ=60)
                                                                              ACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCT                 >  SRR4302533.4513614/1‑94 (MQ=60)
                                                                               CCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTA                >  SRR4302533.3343577/1‑94 (MQ=60)
                                                                                    GGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCC           <  SRR4302533.2555503/94‑1 (MQ=60)
                                                                                    GGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCC           <  SRR4302533.3954338/94‑1 (MQ=60)
                                                                                      CGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCAC         >  SRR4302533.2040142/1‑94 (MQ=60)
                                                                                        ACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCT       <  SRR4302533.5366639/94‑1 (MQ=60)
                                                                                         CCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTG      <  SRR4302533.4630313/94‑1 (MQ=60)
                                                                                         CCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTG      >  SRR4302533.3503577/1‑94 (MQ=60)
                                                                                          CTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGT     >  SRR4302533.114306/1‑94 (MQ=60)
                                                                                           TGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTC    <  SRR4302533.1288184/94‑1 (MQ=60)
                                                                                             CTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAG  >  SRR4302533.3654126/1‑94 (MQ=60)
                                                                                             |                                                                                             
GAATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAG  >  NC_000913/4244450‑4244636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑