Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,295,417 G→A P510L (CCG→CTG)  fdhF ← selenopolypeptide subunit of formate dehydrogenase H

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,295,4170GA99.8% 1465.5 / ‑5.6 396P510L (CCG→CTG) fdhFselenopolypeptide subunit of formate dehydrogenase H
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (215/180);  total (215/181)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

Only 100 of 404 total aligned reads displayed.

GTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATT  >  NC_000913/4295327‑4295506
                                                                                          |                                                                                         
gTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCagg                                                                                         >  1:4543255/1‑93 (MQ=255)
gTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCagg                                                                                         <  2:3383565/93‑1 (MQ=255)
 tCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGc                                                                                        <  1:6589838/93‑1 (MQ=255)
 tCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGc                                                                                        >  1:6279435/1‑93 (MQ=255)
 tCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGc                                                                                        >  1:6164943/1‑93 (MQ=255)
 tCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGc                                                                                        >  1:7371544/1‑93 (MQ=255)
 gccccTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGc                                                                                        <  2:5451986/92‑1 (MQ=255)
  ccccTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGca                                                                                       >  2:6885741/1‑93 (MQ=255)
      tGATCGGCATCTGAAGTGTAGCGACAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGcacaaa                                                                                   <  1:8983123/93‑3 (MQ=255)
      tGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGa                                                                                   >  2:3598974/1‑93 (MQ=255)
      tGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGa                                                                                   >  1:69191/1‑93 (MQ=255)
      tGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGa                                                                                   <  1:1941114/93‑1 (MQ=255)
        aTCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATg                                                                                 >  2:7344870/1‑93 (MQ=255)
        aTCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATg                                                                                 >  2:9579780/1‑93 (MQ=255)
         tCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATgt                                                                                >  2:2068671/1‑92 (MQ=255)
         tCGGCATCTGAAGTATCGAGGCAAGGGCCCTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGa                                                                                >  1:6104184/1‑93 (MQ=255)
           ggCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACg                                                                              >  2:2476410/1‑93 (MQ=255)
            gCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGc                                                                             >  2:326679/1‑93 (MQ=255)
             cATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCa                                                                            >  2:4648135/1‑93 (MQ=255)
             cATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCa                                                                            >  1:1747814/1‑93 (MQ=255)
              aTCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCaa                                                                           >  2:3958522/1‑93 (MQ=255)
               tCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAAc                                                                          >  1:9971211/1‑93 (MQ=255)
               tCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAAc                                                                          <  1:2348962/93‑1 (MQ=255)
                 tGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTc                                                                        >  2:9216059/1‑93 (MQ=255)
                 tGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTc                                                                        <  2:7014264/93‑1 (MQ=255)
                 tGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTc                                                                        <  2:1536399/93‑1 (MQ=255)
                  gAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCa                                                                       <  2:4484005/93‑1 (MQ=255)
                  gAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCa                                                                       <  1:7680426/93‑1 (MQ=255)
                  gAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCa                                                                       <  1:1538258/93‑1 (MQ=255)
                    aGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATc                                                                     >  1:5028102/1‑93 (MQ=255)
                    aGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATc                                                                     <  2:7995954/93‑1 (MQ=255)
                      tATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATccc                                                                   <  1:4815810/93‑1 (MQ=255)
                      tATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATccc                                                                   <  1:421045/93‑1 (MQ=255)
                      gaTCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATccc                                                                   <  1:406365/92‑1 (MQ=255)
                        tCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAg                                                                 >  2:4998950/1‑93 (MQ=255)
                         cgcgGCAAGGCCACTGAATGAAGCTCAGTGCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGa                                                                <  2:230493/93‑1 (MQ=255)
                          gcgGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGAt                                                               <  2:4888951/93‑1 (MQ=255)
                          gcgGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGAt                                                               >  2:7531583/1‑93 (MQ=255)
                           cgGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGAtc                                                              <  2:9390159/93‑1 (MQ=255)
                            ggCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGAtct                                                             >  1:9573865/1‑93 (MQ=255)
                             gCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGAtctc                                                            >  1:3457609/1‑93 (MQ=255)
                             gCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGAtctc                                                            >  1:1163095/1‑93 (MQ=255)
                                 ggCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTgg                                                        >  2:6396606/1‑93 (MQ=255)
                                 ggCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTgg                                                        >  2:8176525/1‑93 (MQ=255)
                                      cTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGtgtt                                                   >  2:9852119/1‑93 (MQ=255)
                                       tgaatgaaGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGtgttg                                                  <  2:8389208/93‑1 (MQ=255)
                                       tgaatgaaGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGtgttg                                                  >  2:2917540/1‑93 (MQ=255)
                                       tgaatgaaGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGtgttg                                                  >  2:1365239/1‑93 (MQ=255)
                                       tgaatgaaGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGtgttg                                                  <  1:8458281/93‑1 (MQ=255)
                                        gaatgaaGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGtgttgt                                                 <  1:7157275/93‑1 (MQ=255)
                                        gaatgaaGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGtgttgt                                                 <  2:1174150/93‑1 (MQ=255)
                                          atgaaGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGtgttgttg                                               >  2:6869694/1‑93 (MQ=255)
                                           tgaaGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGtgttgttgt                                              >  2:3026566/1‑93 (MQ=255)
                                           tgaaGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGtgttgttgt                                              >  1:5806348/1‑93 (MQ=255)
                                           tgaaGCCCAGTTCGCCCATTTCCTCGTAAGTCGCACCGTAGAAATCCAGGCCCAGATGACGCAACTCATCCCAGATCTCCTGGGtgttgttgt                                              <  1:78595/93‑1 (MQ=255)
                                            gaaGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTa                                             <  2:1892040/93‑1 (MQ=255)
                                            gaaGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTa                                             <  1:5581291/93‑1 (MQ=255)
                                               gCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTg                                          >  2:5662307/1‑93 (MQ=255)
                                               gCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTg                                          >  2:1413421/1‑93 (MQ=255)
                                               gCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTg                                          >  1:5651016/1‑93 (MQ=255)
                                                cccAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGc                                         <  2:6141846/93‑1 (MQ=255)
                                                   aGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATc                                      <  2:6746185/93‑1 (MQ=255)
                                                    gTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCg                                     >  2:7159235/1‑93 (MQ=255)
                                                    gTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCg                                     >  1:441923/1‑93 (MQ=255)
                                                    gTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCg                                     >  1:9088253/1‑93 (MQ=255)
                                                     ttCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCgg                                    >  1:248602/1‑93 (MQ=255)
                                                      tCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGa                                   >  1:5183935/1‑93 (MQ=255)
                                                      tCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGa                                   <  2:9083900/93‑1 (MQ=255)
                                                        gCCCATTTTCTCGTAAGTCGCGCCGTAGAAATCCAGGCACAGATGACGCAGCTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATa                                 <  1:9508934/93‑1 (MQ=255)
                                                            aTTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAAccc                             >  2:743322/1‑93 (MQ=255)
                                                            aTTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAAccc                             >  1:8078459/1‑93 (MQ=255)
                                                             ttttCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCa                            >  2:10088/1‑93 (MQ=255)
                                                             ttttCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCa                            <  1:3289249/93‑1 (MQ=255)
                                                             ttttCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCa                            >  1:1050351/1‑93 (MQ=255)
                                                               ttCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATa                          <  2:5356913/93‑1 (MQ=255)
                                                               ttCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATa                          <  1:870949/93‑1 (MQ=255)
                                                                tctcGTAAGTCTCCCCGTAGAAATCCAGGCACAGATGACGCAACTCATCACAGATCTCCTGCGTGTTGTTGTAGTGCATCAGATAGCCCATAc                         <  2:6239000/93‑1 (MQ=255)
                                                                tctcGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATAc                         <  1:8260739/93‑1 (MQ=255)
                                                                 ctcGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACg                        <  2:4814753/93‑1 (MQ=255)
                                                                  tcGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACgg                       <  1:5265797/93‑1 (MQ=255)
                                                                  tcGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACgg                       <  2:6680049/93‑1 (MQ=255)
                                                                   cGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACggg                      <  1:3695979/93‑1 (MQ=255)
                                                                    gTAAGTCGCACCGTAGAAATCCACGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGt                     <  2:5496184/93‑1 (MQ=255)
                                                                     tAAGTCGCACCGTAGCACTCCAGGCACAGATGACGCACCTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTg                    <  2:5924215/93‑1 (MQ=255)
                                                                      aaGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTgg                   <  1:3910022/93‑1 (MQ=255)
                                                                       aGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGc                  <  1:7610648/93‑1 (MQ=255)
                                                                       aGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGc                  <  2:7864689/93‑1 (MQ=255)
                                                                       aGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGc                  <  1:885475/93‑1 (MQ=255)
                                                                         tCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGa                <  1:2071660/93‑1 (MQ=255)
                                                                         tCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGa                <  2:3681241/93‑1 (MQ=255)
                                                                             cccGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTc            <  1:9387694/92‑1 (MQ=255)
                                                                             aCCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTc            <  2:793074/93‑1 (MQ=255)
                                                                              ccGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCa           <  2:8862847/93‑1 (MQ=255)
                                                                                gTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACt         >  1:2624781/1‑93 (MQ=255)
                                                                                gTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACt         <  1:982262/93‑1 (MQ=255)
                                                                                gTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACt         <  2:6535589/93‑1 (MQ=255)
                                                                                    aaaTCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACtgatg     <  1:2903767/93‑1 (MQ=255)
                                                                                     aaTCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACtgatga    >  2:8543315/1‑93 (MQ=255)
                                                                                     aaTCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACtgatga    <  1:1276735/93‑1 (MQ=255)
                                                                                       tCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGAtt  <  2:4575193/93‑1 (MQ=255)
                                                                                          |                                                                                         
GTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATT  >  NC_000913/4295327‑4295506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 201 total aligned reads displayed.
GAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTGCC  >  NC_000913/4295324‑4295510
                                                                                             |                                                                                             
 AAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAG                                                                                              <  SRR4302540.5620288/94‑1 (MQ=60)
 AAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAG                                                                                              <  SRR4302540.1513697/94‑1 (MQ=60)
  AGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGG                                                                                             >  SRR4302540.4592418/1‑94 (MQ=60)
   GTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGC                                                                                            >  SRR4302540.6233636/1‑94 (MQ=60)
   GTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGC                                                                                            >  SRR4302540.7453711/1‑94 (MQ=60)
   GTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGC                                                                                            >  SRR4302540.6349312/1‑94 (MQ=60)
    TCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCA                                                                                           <  SRR4302540.6662825/94‑1 (MQ=60)
     CCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCAC                                                                                          >  SRR4302540.1812433/1‑94 (MQ=60)
     CCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCAC                                                                                          <  SRR4302540.6337059/94‑1 (MQ=60)
     CCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCAC                                                                                          <  SRR4302540.6337060/94‑1 (MQ=60)
      CCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACA                                                                                         <  SRR4302540.3170696/94‑1 (MQ=60)
       CCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAG                                                                                        >  SRR4302540.275619/1‑94 (MQ=60)
       CCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAG                                                                                        >  SRR4302540.9636242/1‑94 (MQ=60)
        CTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGA                                                                                       >  SRR4302540.69888/1‑94 (MQ=60)
        CTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGA                                                                                       >  SRR4302540.64966/1‑94 (MQ=60)
        CTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGA                                                                                       >  SRR4302540.1688116/1‑94 (MQ=60)
        CTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGA                                                                                       <  SRR4302540.755539/94‑1 (MQ=60)
        CTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGA                                                                                       >  SRR4302540.2324765/1‑94 (MQ=60)
         TGATCGGCATCTGAAGTGTAGCGACAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAAAT                                                                                      <  SRR4302540.9090302/94‑1 (MQ=60)
         TGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGAT                                                                                      >  SRR4302540.9119883/1‑94 (MQ=60)
         TGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGAT                                                                                      <  SRR4302540.9153750/94‑1 (MQ=60)
         TGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGAT                                                                                      <  SRR4302540.1962249/94‑1 (MQ=60)
         TGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGAT                                                                                      >  SRR4302540.1714412/1‑94 (MQ=60)
         TGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGAT                                                                                      >  SRR4302540.526484/1‑94 (MQ=60)
         TGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATACAGGCACAGAT                                                                                      >  SRR4302540.1886271/1‑94 (MQ=60)
         GGATCGGCATCTGAAGTATCGCGGCAAGGACACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGAT                                                                                      <  SRR4302540.8706176/94‑1 (MQ=60)
          GATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATG                                                                                     >  SRR4302540.4156267/1‑94 (MQ=60)
          GATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATG                                                                                     >  SRR4302540.122037/1‑94 (MQ=60)
          GATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATG                                                                                     >  SRR4302540.5521714/1‑94 (MQ=60)
          GATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATG                                                                                     >  SRR4302540.3113324/1‑94 (MQ=60)
           ATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGA                                                                                    >  SRR4302540.5097941/1‑94 (MQ=60)
           ATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGA                                                                                    <  SRR4302540.8855733/94‑1 (MQ=60)
           ATCGGCATCTGAAGTATCGAGGCAAGGGCCCTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGA                                                                                    >  SRR4302540.6172157/1‑94 (MQ=60)
            TCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGAC                                                                                   <  SRR4302540.5021259/94‑1 (MQ=60)
            TCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGAC                                                                                   <  SRR4302540.2432851/94‑1 (MQ=60)
            TCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGAC                                                                                   >  SRR4302540.5865587/1‑94 (MQ=60)
             CGGCATCTGAAGTATCGAGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACG                                                                                  <  SRR4302540.2070794/94‑1 (MQ=60)
             CGCCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACG                                                                                  <  SRR4302540.9888949/94‑1 (MQ=60)
              GGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGC                                                                                 >  SRR4302540.9042135/1‑94 (MQ=60)
              GGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGC                                                                                 >  SRR4302540.4630673/1‑94 (MQ=60)
               GCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCA                                                                                <  SRR4302540.10012366/94‑1 (MQ=60)
               GCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCA                                                                                >  SRR4302540.1766740/1‑94 (MQ=60)
                 ATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGTCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAAC                                                                              >  SRR4302540.5541542/1‑94 (MQ=60)
                 ATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAAC                                                                              >  SRR4302540.773657/1‑94 (MQ=60)
                 ATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAAC                                                                              >  SRR4302540.2717454/1‑94 (MQ=60)
                 ATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAAC                                                                              >  SRR4302540.10087525/1‑94 (MQ=60)
                 ATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAAC                                                                              >  SRR4302540.7948790/1‑94 (MQ=60)
                 ATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAAC                                                                              >  SRR4302540.7948794/1‑94 (MQ=60)
                  TCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACT                                                                             <  SRR4302540.2374261/94‑1 (MQ=60)
                   CTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTC                                                                            >  SRR4302540.3873842/1‑94 (MQ=60)
                   CTGAAGTATCGCGGCAAGCCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTC                                                                            >  SRR4302540.1274855/1‑94 (MQ=60)
                    TGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCA                                                                           >  SRR4302540.9647597/1‑94 (MQ=60)
                    TGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCA                                                                           >  SRR4302540.7460981/1‑94 (MQ=60)
                     GAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCAT                                                                          <  SRR4302540.7767357/94‑1 (MQ=60)
                     GAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCAT                                                                          <  SRR4302540.5805560/94‑1 (MQ=60)
                     GAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCAT                                                                          <  SRR4302540.8993507/94‑1 (MQ=60)
                     GAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCAT                                                                          <  SRR4302540.4228443/94‑1 (MQ=60)
                     GAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCAT                                                                          <  SRR4302540.1555268/94‑1 (MQ=60)
                      AAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATC                                                                         >  SRR4302540.5082519/1‑94 (MQ=60)
                      AAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATC                                                                         >  SRR4302540.9454785/1‑94 (MQ=60)
                       AGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCC                                                                        >  SRR4302540.4736866/1‑94 (MQ=60)
                        GTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCC                                                                       >  SRR4302540.8542737/1‑94 (MQ=60)
                        GTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCC                                                                       <  SRR4302540.4830786/94‑1 (MQ=60)
                         TATCGCGGCAAGGCCCCTGAATGAAGCCCATTTCG‑CCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAG                                                                     <  SRR4302540.2046605/94‑1 (MQ=60)
                         TATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCA                                                                      <  SRR4302540.7452416/94‑1 (MQ=60)
                         TATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCA                                                                      <  SRR4302540.425475/94‑1 (MQ=60)
                         TATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCA                                                                      <  SRR4302540.4867353/94‑1 (MQ=60)
                         GATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCA                                                                      <  SRR4302540.410615/94‑1 (MQ=60)
                         GATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCA                                                                      <  SRR4302540.1961152/94‑1 (MQ=60)
                            CGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGAT                                                                   <  SRR4302540.2467332/94‑1 (MQ=60)
                             GCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACAGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATC                                                                  >  SRR4302540.2823719/1‑94 (MQ=60)
                              CGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCT                                                                 >  SRR4302540.9686952/1‑94 (MQ=60)
                              CGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCT                                                                 <  SRR4302540.9630969/94‑1 (MQ=60)
                               GGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTC                                                                >  SRR4302540.3495242/1‑94 (MQ=60)
                               GGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTC                                                                >  SRR4302540.1175170/1‑94 (MQ=60)
                                GCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCC                                                               >  SRR4302540.8891911/1‑94 (MQ=60)
                                GCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCC                                                               >  SRR4302540.2297638/1‑94 (MQ=60)
                                 CAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCT                                                              <  SRR4302540.8751335/94‑1 (MQ=60)
                                 CAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCT                                                              >  SRR4302540.4355276/1‑94 (MQ=60)
                                 CAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCT                                                              <  SRR4302540.7811338/94‑1 (MQ=60)
                                  AAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTG                                                             <  SRR4302540.6364049/94‑1 (MQ=60)
                                  AAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTG                                                             <  SRR4302540.6364050/94‑1 (MQ=60)
                                   AGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGG                                                            <  SRR4302540.3452838/94‑1 (MQ=60)
                                     GCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGT                                                          <  SRR4302540.5036918/94‑1 (MQ=60)
                                     GCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGT                                                          >  SRR4302540.3099108/1‑94 (MQ=60)
                                      CCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTG                                                         >  SRR4302540.617882/1‑94 (MQ=60)
                                      CCAATGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTG                                                         <  SRR4302540.7701874/94‑1 (MQ=60)
                                         CTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTG                                                      >  SRR4302540.7223649/1‑94 (MQ=60)
                                         CTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTG                                                      >  SRR4302540.1009869/1‑94 (MQ=60)
                                          TGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGT                                                     <  SRR4302540.8556491/94‑1 (MQ=60)
                                           GAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTT                                                    >  SRR4302540.7043250/1‑94 (MQ=60)
                                           GAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTT                                                    <  SRR4302540.7237047/94‑1 (MQ=60)
                                            AATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTG                                                   <  SRR4302540.6173567/94‑1 (MQ=60)
                                            AATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTG                                                   <  SRR4302540.6173566/94‑1 (MQ=60)
                                             ATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGT                                                  >  SRR4302540.8006413/1‑94 (MQ=60)
                                             ATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGT                                                  >  SRR4302540.5870505/1‑94 (MQ=60)
                                              TGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTA                                                 <  SRR4302540.5299164/94‑1 (MQ=60)
                                              TGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATACCAGATCTCCTGGGTGTTGTTGTA                                                 >  SRR4302540.4492326/1‑94 (MQ=60)
                                              TGAAGCCCAGTTCGCCCATTTCCTCGTAAGTCGCACCGTAGAAATCCAGGCCCAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTA                                                 <  SRR4302540.79421/94‑1 (MQ=60)
                                               GAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAG                                                <  SRR4302540.1976826/94‑1 (MQ=60)
                                               GAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAG                                                <  SRR4302540.8272870/94‑1 (MQ=60)
                                               GAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAG                                                <  SRR4302540.5642729/94‑1 (MQ=60)
                                                AAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTGGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGT                                               <  SRR4302540.4441351/94‑1 (MQ=60)
                                                 AGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTG                                              >  SRR4302540.5713232/1‑94 (MQ=60)
                                                   CCCAGTTCGCCCATTTTCTCGTAAGTCGCACAGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCA                                            <  SRR4302540.1113632/94‑1 (MQ=60)
                                                    CCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCAT                                           >  SRR4302540.253090/1‑94 (MQ=60)
                                                    CCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCAT                                           <  SRR4302540.4094141/94‑1 (MQ=60)
                                                    CCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCAT                                           >  SRR4302540.8713002/1‑94 (MQ=60)
                                                    CCAGTTCGCCCATTTTCTCGTAAGTCGAACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGATGTAGTTCAT                                           >  SRR4302540.7324262/1‑94 (MQ=60)
                                                     CAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATC                                          >  SRR4302540.493569/1‑94 (MQ=60)
                                                      AGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCG                                         >  SRR4302540.9197435/1‑94 (MQ=60)
                                                      AGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCG                                         >  SRR4302540.9992945/1‑94 (MQ=60)
                                                      AGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCG                                         >  SRR4302540.446631/1‑94 (MQ=60)
                                                      AGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCG                                         <  SRR4302540.2132869/94‑1 (MQ=60)
                                                      AGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCG                                         >  SRR4302540.3784426/1‑94 (MQ=60)
                                                       GTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGG                                        >  SRR4302540.251010/1‑94 (MQ=60)
                                                        TTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGA                                       >  SRR4302540.5240368/1‑94 (MQ=60)
                                                         TCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGAT                                      <  SRR4302540.5843720/94‑1 (MQ=60)
                                                         TCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGAT                                      <  SRR4302540.3365338/94‑1 (MQ=60)
                                                          CGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATA                                     <  SRR4302540.987327/94‑1 (MQ=60)
                                                           GGCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAGCTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAA                                    <  SRR4302540.6700495/94‑1 (MQ=60)
                                                           GCCCATTTTCTCGTAAGTCGCGCCGTAGAAATCCAGGCACAGATGACGCAGCTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAA                                    <  SRR4302540.9621576/94‑1 (MQ=60)
                                                           GCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAA                                    >  SRR4302540.9294983/1‑94 (MQ=60)
                                                           GCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAA                                    <  SRR4302540.6062274/94‑1 (MQ=60)
                                                             CCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACC                                  >  SRR4302540.9617459/1‑94 (MQ=60)
                                                              CATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCC                                 <  SRR4302540.5674343/94‑1 (MQ=60)
                                                              CATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCC                                 >  SRR4302540.8172215/1‑94 (MQ=60)
                                                              CATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCC                                 <  SRR4302540.10079468/94‑1 (MQ=60)
                                                               ATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCA                                >  SRR4302540.5673163/1‑94 (MQ=60)
                                                               ATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCA                                >  SRR4302540.3447482/1‑94 (MQ=60)
                                                               ATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCA                                >  SRR4302540.1061038/1‑94 (MQ=60)
                                                                TTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCAT                               <  SRR4302540.3325230/94‑1 (MQ=60)
                                                                 TTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATA                              >  SRR4302540.3788231/1‑94 (MQ=60)
                                                                  TTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATAC                             <  SRR4302540.880195/94‑1 (MQ=60)
                                                                  TTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATAC                             <  SRR4302540.9578072/94‑1 (MQ=60)
                                                                  TTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATAC                             <  SRR4302540.354590/94‑1 (MQ=60)
                                                                   TCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACG                            <  SRR4302540.8356735/94‑1 (MQ=60)
                                                                   TCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACG                            <  SRR4302540.8356740/94‑1 (MQ=60)
                                                                   TCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACG                            >  SRR4302540.1389499/1‑94 (MQ=60)
                                                                    CTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGG                           >  SRR4302540.9181323/1‑94 (MQ=60)
                                                                    CTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGG                           >  SRR4302540.1152922/1‑94 (MQ=60)
                                                                    CTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGG                           <  SRR4302540.5879902/94‑1 (MQ=60)
                                                                     TCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGG                          <  SRR4302540.5323263/94‑1 (MQ=60)
                                                                     TCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGG                          <  SRR4302540.524792/94‑1 (MQ=60)
                                                                      CGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGT                         <  SRR4302540.3736334/94‑1 (MQ=60)
                                                                      CGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGT                         <  SRR4302540.7761191/94‑1 (MQ=60)
                                                                      CGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGG                         >  SRR4302540.8962403/1‑94 (MQ=60)
                                                                       GTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTG                        <  SRR4302540.4057816/94‑1 (MQ=60)
                                                                       GTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTG                        >  SRR4302540.1072404/1‑94 (MQ=60)
                                                                       GTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTG                        >  SRR4302540.9581894/1‑94 (MQ=60)
                                                                       GTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTG                        <  SRR4302540.3700522/94‑1 (MQ=60)
                                                                       GTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTG                        <  SRR4302540.5178831/94‑1 (MQ=60)
                                                                       GTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTG                        <  SRR4302540.1424633/94‑1 (MQ=60)
                                                                       GTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTG                        >  SRR4302540.8337454/1‑94 (MQ=60)
                                                                        TAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGG                       >  SRR4302540.2439552/1‑94 (MQ=60)
                                                                        TAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGG                       <  SRR4302540.6904579/94‑1 (MQ=60)
                                                                         AAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGC                      <  SRR4302540.3952395/94‑1 (MQ=60)
                                                                         AAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGC                      <  SRR4302540.8125795/94‑1 (MQ=60)
                                                                          AGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCG                     <  SRR4302540.7696185/94‑1 (MQ=60)
                                                                          AGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCG                     <  SRR4302540.176440/94‑1 (MQ=60)
                                                                          AGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCG                     <  SRR4302540.894870/94‑1 (MQ=60)
                                                                          AGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCG                     >  SRR4302540.4552435/1‑94 (MQ=60)
                                                                            TCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGAT                   <  SRR4302540.2093939/94‑1 (MQ=60)
                                                                             CGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATT                  >  SRR4302540.5085750/1‑94 (MQ=60)
                                                                              GCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTT                 <  SRR4302540.4068822/94‑1 (MQ=60)
                                                                                CCCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCA               <  SRR4302540.9499423/94‑1 (MQ=60)
                                                                                 CCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCAC              <  SRR4302540.6046370/94‑1 (MQ=60)
                                                                                  CGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACT             >  SRR4302540.5587943/1‑94 (MQ=60)
                                                                                  CGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACT             >  SRR4302540.2653167/1‑94 (MQ=60)
                                                                                  CGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACT             >  SRR4302540.2653166/1‑94 (MQ=60)
                                                                                  CGTAGAAAGCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACT             <  SRR4302540.5645372/94‑1 (MQ=60)
                                                                                   GTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTG            >  SRR4302540.6674467/1‑94 (MQ=60)
                                                                                   GTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTG            >  SRR4302540.9769845/1‑94 (MQ=60)
                                                                                   GTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTG            <  SRR4302540.992318/94‑1 (MQ=60)
                                                                                    TAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGA           <  SRR4302540.9326621/94‑1 (MQ=60)
                                                                                     AGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGAT          >  SRR4302540.44250/1‑94 (MQ=60)
                                                                                     AGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGAT          >  SRR4302540.8923385/1‑94 (MQ=60)
                                                                                     AGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGAT          <  SRR4302540.10114276/94‑1 (MQ=60)
                                                                                      GAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATG         <  SRR4302540.4485422/94‑1 (MQ=60)
                                                                                       AAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGT        >  SRR4302540.7278143/1‑94 (MQ=60)
                                                                                       AAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGA        >  SRR4302540.709008/1‑94 (MQ=60)
                                                                                       AAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGA        <  SRR4302540.2935464/94‑1 (MQ=60)
                                                                                       AAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGA        <  SRR4302540.253983/94‑1 (MQ=60)
                                                                                        AATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGAT       >  SRR4302540.8256110/1‑94 (MQ=60)
                                                                                        AATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGAT       >  SRR4302540.4135262/1‑94 (MQ=60)
                                                                                        AATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGAT       <  SRR4302540.7984029/94‑1 (MQ=60)
                                                                                        AATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGAT       <  SRR4302540.1289896/94‑1 (MQ=60)
                                                                                        AATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGAT       <  SRR4302540.1456437/94‑1 (MQ=60)
                                                                                        AATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGAT       >  SRR4302540.355428/1‑94 (MQ=60)
                                                                                        AATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGAT       <  SRR4302540.1289897/94‑1 (MQ=60)
                                                                                          TCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTT     <  SRR4302540.6313043/94‑1 (MQ=60)
                                                                                          TCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTT     <  SRR4302540.8469542/94‑1 (MQ=60)
                                                                                          TCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTT     <  SRR4302540.7630044/94‑1 (MQ=60)
                                                                                          TCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTT     <  SRR4302540.5730268/94‑1 (MQ=60)
                                                                                          TCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTT     >  SRR4302540.616772/1‑94 (MQ=60)
                                                                                           CCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGGGATTTCACTGATGATTTG    >  SRR4302540.3232480/1‑94 (MQ=60)
                                                                                           CCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTG    >  SRR4302540.435731/1‑94 (MQ=60)
                                                                                           CCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTG    >  SRR4302540.4610663/1‑94 (MQ=60)
                                                                                           CCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTG    <  SRR4302540.10088400/94‑1 (MQ=60)
                                                                                             AGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTGCC  >  SRR4302540.8378665/1‑94 (MQ=60)
                                                                                             |                                                                                             
GAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTGCC  >  NC_000913/4295324‑4295510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑