Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,295,417 G→A P510L (CCG→CTG)  fdhF ← selenopolypeptide subunit of formate dehydrogenase H

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,295,4170GA100.0% 951.7 / NA 252P510L (CCG→CTG) fdhFselenopolypeptide subunit of formate dehydrogenase H
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (132/120);  total (132/120)

Only 100 of 257 total aligned reads displayed.

GTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATT  >  NC_000913/4295327‑4295506
                                                                                          |                                                                                         
gTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCagg                                                                                         >  1:1010714/1‑93 (MQ=255)
  ccccTGATCGTCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGca                                                                                       <  2:4173385/93‑1 (MQ=255)
    ccTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGcaca                                                                                     <  1:4947066/93‑1 (MQ=255)
    ccTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGcaca                                                                                     >  1:319022/1‑93 (MQ=255)
    ccTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGcaca                                                                                     <  2:2782175/93‑1 (MQ=255)
    ccTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGcaca                                                                                     >  2:3982214/1‑93 (MQ=255)
      tGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGTCATCCAGGCACCGa                                                                                   >  2:4816022/1‑93 (MQ=255)
      tGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGa                                                                                   <  2:2298703/93‑1 (MQ=255)
      tGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGa                                                                                   >  1:6256074/1‑93 (MQ=255)
      tGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGa                                                                                   >  2:1805440/1‑93 (MQ=255)
      tGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGa                                                                                   >  1:1906827/1‑93 (MQ=255)
       gATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGAt                                                                                  <  1:6080638/93‑1 (MQ=255)
       gATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGAt                                                                                  >  1:6186375/1‑93 (MQ=255)
       gATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGAt                                                                                  >  1:1065389/1‑93 (MQ=255)
         tCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGa                                                                                <  1:5540931/93‑1 (MQ=255)
          cGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGAc                                                                               <  2:869353/93‑1 (MQ=255)
          cGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGAc                                                                               <  2:3128205/93‑1 (MQ=255)
             cATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCa                                                                            >  1:5785870/1‑93 (MQ=255)
              aTCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCaa                                                                           >  2:1115352/1‑93 (MQ=255)
              aTCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCaa                                                                           >  1:1692288/1‑93 (MQ=255)
              aTCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCaa                                                                           >  2:350822/1‑93 (MQ=255)
               tCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAAc                                                                          >  1:4865169/1‑93 (MQ=255)
               tCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAAc                                                                          >  2:4305100/1‑93 (MQ=255)
                cTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACt                                                                         <  1:5680133/93‑1 (MQ=255)
                cTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACt                                                                         <  2:6097299/93‑1 (MQ=255)
                 tGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCCTTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTc                                                                        >  2:301941/1‑93 (MQ=255)
                 tGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTc                                                                        >  2:5521220/1‑93 (MQ=255)
                  gAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCa                                                                       >  1:5869287/1‑93 (MQ=255)
                  gAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCa                                                                       <  2:5782723/93‑1 (MQ=255)
                  gAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCa                                                                       >  2:1965121/1‑93 (MQ=255)
                   aaGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCAt                                                                      <  1:4306224/93‑1 (MQ=255)
                   aaGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCAt                                                                      >  2:5743169/1‑93 (MQ=255)
                    aGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATc                                                                     <  1:1722373/93‑1 (MQ=255)
                     gTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATcc                                                                    >  2:2014324/1‑93 (MQ=255)
                       aTCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCa                                                                  <  2:2190619/93‑1 (MQ=255)
                        tCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAg                                                                 >  1:3793042/1‑93 (MQ=255)
                         cgcgGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGa                                                                <  1:867477/93‑1 (MQ=255)
                         cgcgGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGa                                                                <  1:3261441/93‑1 (MQ=255)
                            ggCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGAtct                                                             <  1:4501715/93‑1 (MQ=255)
                             gCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGAtctc                                                            >  1:3620607/1‑93 (MQ=255)
                              cAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTcc                                                           <  2:5373554/93‑1 (MQ=255)
                                aGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTg                                                         >  1:102134/1‑93 (MQ=255)
                                aGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTg                                                         >  2:2455834/1‑93 (MQ=255)
                                aGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTg                                                         <  1:2594056/93‑1 (MQ=255)
                                aGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTg                                                         <  1:2349965/93‑1 (MQ=255)
                                aGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTg                                                         >  2:378093/1‑93 (MQ=255)
                                aGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTg                                                         <  1:3445948/93‑1 (MQ=255)
                                  gCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTggg                                                       >  1:1796288/1‑93 (MQ=255)
                                   ccACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGgt                                                      <  2:5165762/93‑1 (MQ=255)
                                    cACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGgtg                                                     >  1:2399384/1‑93 (MQ=255)
                                    cACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGgtg                                                     >  1:3880916/1‑93 (MQ=255)
                                     aCTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGgtgt                                                    >  2:2662201/1‑93 (MQ=255)
                                       tgaatgaaGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGtgttg                                                  <  2:3418689/93‑1 (MQ=255)
                                        gaatgaaGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGtgttgt                                                 >  1:977203/1‑93 (MQ=255)
                                         aatgaaGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGtgttgtt                                                <  2:2820691/93‑1 (MQ=255)
                                          atgaaGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGtgttgttg                                               >  1:2712630/1‑93 (MQ=255)
                                              aGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGt                                           <  1:2284333/93‑1 (MQ=255)
                                               gCCCAGTTCACCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTg                                          <  2:5622193/93‑1 (MQ=255)
                                                cccAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGc                                         <  2:1307739/93‑1 (MQ=255)
                                                cccAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGc                                         <  2:3318592/93‑1 (MQ=255)
                                                cccAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGc                                         <  1:1104824/93‑1 (MQ=255)
                                                 ccAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCa                                        >  1:2875154/1‑93 (MQ=255)
                                                 ccAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCa                                        >  1:4208071/1‑93 (MQ=255)
                                                  cAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCAt                                       <  1:5934815/93‑1 (MQ=255)
                                                    gTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCg                                     >  1:146640/1‑93 (MQ=255)
                                                    gTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCg                                     >  2:2880547/1‑93 (MQ=255)
                                                    gTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCg                                     <  1:373368/93‑1 (MQ=255)
                                                     ttCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCgg                                    <  2:160881/93‑1 (MQ=255)
                                                      tCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGa                                   <  1:1220999/93‑1 (MQ=255)
                                                      tCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGa                                   <  2:5232476/93‑1 (MQ=255)
                                                      tCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGa                                   >  2:3950261/1‑93 (MQ=255)
                                                       cGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGAt                                  <  1:1281214/93‑1 (MQ=255)
                                                        gCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATa                                 >  1:1393073/1‑93 (MQ=255)
                                                        gCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATa                                 <  2:4643855/93‑1 (MQ=255)
                                                          ccATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGGTGACGCAACTCATCCCAGATCTCCTGGGCGTTGTTGTAGTGCATCGGATAAc                               >  1:5274919/1‑93 (MQ=255)
                                                            aTTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAAccc                             >  2:2147491/1‑93 (MQ=255)
                                                             ttttCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCa                            <  2:1580705/93‑1 (MQ=255)
                                                                tctcGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATAc                         <  2:5462249/93‑1 (MQ=255)
                                                                  tcGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACgg                       <  1:188454/93‑1 (MQ=255)
                                                                  tcGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACgg                       >  1:411719/1‑93 (MQ=255)
                                                                   cGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACggg                      >  1:3995948/1‑93 (MQ=255)
                                                                    gTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGt                     <  2:3532210/93‑1 (MQ=255)
                                                                     tAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTg                    >  1:4758153/1‑93 (MQ=255)
                                                                      aaGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTgg                   >  1:5294475/1‑93 (MQ=255)
                                                                        gTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCg                 >  1:5412228/1‑93 (MQ=255)
                                                                        gTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCg                 >  2:5384049/1‑93 (MQ=255)
                                                                         tCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGa                <  2:735337/93‑1 (MQ=255)
                                                                         tCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGa                >  1:3365749/1‑93 (MQ=255)
                                                                          cGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGAt               >  2:4943079/1‑93 (MQ=255)
                                                                           gCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGAtt              >  2:1356356/1‑93 (MQ=255)
                                                                              ccGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCa           <  2:5302018/93‑1 (MQ=255)
                                                                              ccGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCa           >  2:803037/1‑93 (MQ=255)
                                                                              ccGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCa           >  2:6226910/1‑93 (MQ=255)
                                                                               cGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCAc          >  2:2385065/1‑93 (MQ=255)
                                                                               cGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCAc          <  1:2145919/93‑1 (MQ=255)
                                                                                 tAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACtg        >  2:4099360/1‑93 (MQ=255)
                                                                                 tAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTATAGTGCATCGGATAACCCATACGGGTGGCGATTTCACtg        <  2:3938924/93‑1 (MQ=255)
                                                                                  aGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACtga       <  1:136092/93‑1 (MQ=255)
                                                                                    aaaTCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACtgatg     <  1:5064892/93‑1 (MQ=255)
                                                                                       tCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGAtt  <  1:537712/93‑1 (MQ=255)
                                                                                          |                                                                                         
GTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATT  >  NC_000913/4295327‑4295506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTGCC  >  NC_000913/4295324‑4295510
                                                                                             |                                                                                             
GAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCA                                                                                               <  SRR4302533.4073406/94‑1 (MQ=60)
GAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCA                                                                                               >  SRR4302533.1173795/1‑94 (MQ=60)
 AAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGTCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAG                                                                                              <  SRR4302533.3404656/94‑1 (MQ=60)
 AAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAG                                                                                              <  SRR4302533.2727323/94‑1 (MQ=60)
 AAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAG                                                                                              <  SRR4302533.730320/94‑1 (MQ=60)
 AAGTCCCCAGATCGGCATCTGAAGTATCGCGGCACGGCCACTAAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAG                                                                                              <  SRR4302533.472858/94‑1 (MQ=60)
  AGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGG                                                                                             >  SRR4302533.1016627/1‑94 (MQ=60)
   GTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGC                                                                                            <  SRR4302533.6320098/94‑1 (MQ=60)
     CCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCAC                                                                                          >  SRR4302533.101957/1‑94 (MQ=60)
      CCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACA                                                                                         <  SRR4302533.4833011/94‑1 (MQ=60)
      CCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACA                                                                                         >  SRR4302533.5950884/1‑94 (MQ=60)
      CCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACA                                                                                         >  SRR4302533.1375398/1‑94 (MQ=60)
      CCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACA                                                                                         >  SRR4302533.320977/1‑94 (MQ=60)
       CCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAG                                                                                        <  SRR4302533.4095639/94‑1 (MQ=60)
       CCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAG                                                                                        <  SRR4302533.4977011/94‑1 (MQ=60)
       CCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAG                                                                                        >  SRR4302533.522779/1‑94 (MQ=60)
        CTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGA                                                                                       >  SRR4302533.6293485/1‑94 (MQ=60)
        CTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGA                                                                                       >  SRR4302533.1918459/1‑94 (MQ=60)
         TGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGAT                                                                                      >  SRR4302533.6223510/1‑94 (MQ=60)
         TGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGAT                                                                                      >  SRR4302533.1071647/1‑94 (MQ=60)
         TGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACAGTAGAAATCCAGGCACAGAT                                                                                      >  SRR4302533.4229790/1‑94 (MQ=60)
          GATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATG                                                                                     >  SRR4302533.1055184/1‑94 (MQ=60)
          GATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATG                                                                                     <  SRR4302533.6117410/94‑1 (MQ=60)
          GATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATG                                                                                     >  SRR4302533.5306610/1‑94 (MQ=60)
           ATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGA                                                                                    <  SRR4302533.1304279/94‑1 (MQ=60)
            TCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGAC                                                                                   <  SRR4302533.5574393/94‑1 (MQ=60)
            TCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGAC                                                                                   <  SRR4302533.5386115/94‑1 (MQ=60)
             CGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACG                                                                                  >  SRR4302533.5417294/1‑94 (MQ=60)
               GCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCA                                                                                >  SRR4302533.5820815/1‑94 (MQ=60)
                CATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAA                                                                               >  SRR4302533.1702541/1‑94 (MQ=60)
                 ATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAAC                                                                              >  SRR4302533.6199199/1‑94 (MQ=60)
                 ATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAAC                                                                              >  SRR4302533.4894652/1‑94 (MQ=60)
                 ATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAAC                                                                              >  SRR4302533.32290/1‑94 (MQ=60)
                   CTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTC                                                                            >  SRR4302533.1605820/1‑94 (MQ=60)
                   CTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTC                                                                            <  SRR4302533.5714441/94‑1 (MQ=60)
                   CTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTC                                                                            <  SRR4302533.566078/94‑1 (MQ=60)
                    TGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCA                                                                           >  SRR4302533.5321610/1‑94 (MQ=60)
                    TGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCA                                                                           <  SRR4302533.951066/94‑1 (MQ=60)
                    TGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCA                                                                           >  SRR4302533.5904761/1‑94 (MQ=60)
                    TGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCA                                                                           >  SRR4302533.1833593/1‑94 (MQ=60)
                    TGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCA                                                                           >  SRR4302533.6068825/1‑94 (MQ=60)
                    TGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCA                                                                           >  SRR4302533.1436369/1‑94 (MQ=60)
                    TGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCA                                                                           >  SRR4302533.38324/1‑94 (MQ=60)
                      AAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATC                                                                         <  SRR4302533.4332134/94‑1 (MQ=60)
                       AGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCC                                                                        <  SRR4302533.1732817/94‑1 (MQ=60)
                       AGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCC                                                                        >  SRR4302533.1716233/1‑94 (MQ=60)
                        GTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCC                                                                       <  SRR4302533.3499953/94‑1 (MQ=60)
                         TATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCA                                                                      >  SRR4302533.6156144/1‑94 (MQ=60)
                          ATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAG                                                                     <  SRR4302533.1916391/94‑1 (MQ=60)
                          ATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAG                                                                     <  SRR4302533.1320248/94‑1 (MQ=60)
                          ATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAG                                                                     >  SRR4302533.3816258/1‑94 (MQ=60)
                            CGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGAT                                                                   <  SRR4302533.1227867/94‑1 (MQ=60)
                            CGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGAT                                                                   <  SRR4302533.872560/94‑1 (MQ=60)
                            CGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGAT                                                                   <  SRR4302533.3281559/94‑1 (MQ=60)
                              TGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCT                                                                 >  SRR4302533.6279940/1‑94 (MQ=60)
                               GGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTC                                                                >  SRR4302533.3642884/1‑94 (MQ=60)
                               GGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTC                                                                <  SRR4302533.4528986/94‑1 (MQ=60)
                                 CAAGTCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCT                                                              <  SRR4302533.3338259/94‑1 (MQ=60)
                                  AAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTG                                                             >  SRR4302533.102770/1‑94 (MQ=60)
                                   AGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGG                                                            <  SRR4302533.2364501/94‑1 (MQ=60)
                                   AGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGG                                                            <  SRR4302533.3467227/94‑1 (MQ=60)
                                   AGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGG                                                            >  SRR4302533.3957157/1‑94 (MQ=60)
                                   AGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGG                                                            <  SRR4302533.2609994/94‑1 (MQ=60)
                                    GGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGG                                                           >  SRR4302533.1807218/1‑94 (MQ=60)
                                     GCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGT                                                          >  SRR4302533.1106217/1‑94 (MQ=60)
                                      CCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTG                                                         >  SRR4302533.3904597/1‑94 (MQ=60)
                                      CCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTG                                                         >  SRR4302533.2414200/1‑94 (MQ=60)
                                          TGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGT                                                     >  SRR4302533.982935/1‑94 (MQ=60)
                                            AATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTG                                                   <  SRR4302533.2426116/94‑1 (MQ=60)
                                            AATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTG                                                   >  SRR4302533.2729286/1‑94 (MQ=60)
                                              TGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTA                                                 >  SRR4302533.4320931/1‑94 (MQ=60)
                                               GAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAG                                                >  SRR4302533.493516/1‑94 (MQ=60)
                                                AAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGT                                               <  SRR4302533.3454799/94‑1 (MQ=60)
                                                 AGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTG                                              >  SRR4302533.5092084/1‑94 (MQ=60)
                                                 AGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTG                                              <  SRR4302533.5764184/94‑1 (MQ=60)
                                                 AGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTG                                              <  SRR4302533.2298471/94‑1 (MQ=60)
                                                   CCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCA                                            <  SRR4302533.4733439/94‑1 (MQ=60)
                                                   CCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCA                                            >  SRR4302533.2892846/1‑94 (MQ=60)
                                                   CCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCA                                            >  SRR4302533.2892845/1‑94 (MQ=60)
                                                   CCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCA                                            >  SRR4302533.4233531/1‑94 (MQ=60)
                                                   CCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCA                                            <  SRR4302533.1111372/94‑1 (MQ=60)
                                                    CCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCAT                                           <  SRR4302533.2377294/94‑1 (MQ=60)
                                                    CCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCAT                                           <  SRR4302533.1908722/94‑1 (MQ=60)
                                                     CAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATC                                          <  SRR4302533.5970694/94‑1 (MQ=60)
                                                     CAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATC                                          >  SRR4302533.5668879/1‑94 (MQ=60)
                                                     CAGTTCGCCCATTTTCTCGTAAGTCGCACCATAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATC                                          <  SRR4302533.349463/94‑1 (MQ=60)
                                                      AGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCG                                         >  SRR4302533.4956832/1‑94 (MQ=60)
                                                      AGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCG                                         >  SRR4302533.147579/1‑94 (MQ=60)
                                                       GTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGG                                        >  SRR4302533.4241292/1‑94 (MQ=60)
                                                       GTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGG                                        <  SRR4302533.375624/94‑1 (MQ=60)
                                                       GTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGG                                        <  SRR4302533.3970707/94‑1 (MQ=60)
                                                         TCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGAT                                      <  SRR4302533.1228325/94‑1 (MQ=60)
                                                          CGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATA                                     <  SRR4302533.1288922/94‑1 (MQ=60)
                                                          CGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATA                                     >  SRR4302533.1401466/1‑94 (MQ=60)
                                                           GCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAA                                    <  SRR4302533.1035708/94‑1 (MQ=60)
                                                           GCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAA                                    <  SRR4302533.4528561/94‑1 (MQ=60)
                                                            CCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGGTGACGCAACTCATCCCAGATCTCCTGGGCGTTGTTGTAGTGCATCGGATAAC                                   >  SRR4302533.5306548/1‑94 (MQ=60)
                                                            CCCATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAAC                                   <  SRR4302533.3280854/94‑1 (MQ=60)
                                                               ATTTTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCA                                >  SRR4302533.5421404/1‑94 (MQ=60)
                                                                  TTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATAC                             <  SRR4302533.2359514/94‑1 (MQ=60)
                                                                  TTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATAC                             >  SRR4302533.5929813/1‑94 (MQ=60)
                                                                  TTCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATAC                             <  SRR4302533.1249499/94‑1 (MQ=60)
                                                                   TCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACG                            >  SRR4302533.3649013/1‑94 (MQ=60)
                                                                   TCTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACG                            <  SRR4302533.3778445/94‑1 (MQ=60)
                                                                    CTCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGG                           >  SRR4302533.414191/1‑94 (MQ=60)
                                                                     TCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGG                          <  SRR4302533.189627/94‑1 (MQ=60)
                                                                     TCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGG                          >  SRR4302533.4020276/1‑94 (MQ=60)
                                                                     TCGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGG                          >  SRR4302533.2744547/1‑94 (MQ=60)
                                                                      CGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGT                         <  SRR4302533.3790248/94‑1 (MQ=60)
                                                                      CGTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGT                         >  SRR4302533.5445053/1‑94 (MQ=60)
                                                                       GTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTG                        >  SRR4302533.4787020/1‑94 (MQ=60)
                                                                       GTAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTG                        >  SRR4302533.1748744/1‑94 (MQ=60)
                                                                        TAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGG                       >  SRR4302533.5326167/1‑94 (MQ=60)
                                                                        TAAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGG                       <  SRR4302533.1117661/94‑1 (MQ=60)
                                                                         AAGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGC                      <  SRR4302533.464570/94‑1 (MQ=60)
                                                                          AGTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCG                     >  SRR4302533.5444772/1‑94 (MQ=60)
                                                                           GTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGA                    >  SRR4302533.3386539/1‑94 (MQ=60)
                                                                           GTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGA                    >  SRR4302533.1546176/1‑94 (MQ=60)
                                                                           GTCGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGA                    <  SRR4302533.721930/94‑1 (MQ=60)
                                                                             CGCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATT                  >  SRR4302533.4106222/1‑94 (MQ=60)
                                                                              GCACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTT                 >  SRR4302533.2180111/1‑94 (MQ=60)
                                                                               CACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTC                <  SRR4302533.2587758/94‑1 (MQ=60)
                                                                               CACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTC                >  SRR4302533.5762597/1‑94 (MQ=60)
                                                                               CACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTC                <  SRR4302533.2623283/94‑1 (MQ=60)
                                                                                CCCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCA               <  SRR4302533.2238792/94‑1 (MQ=60)
                                                                                ACCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCA               >  SRR4302533.1431288/1‑94 (MQ=60)
                                                                                 CCGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCAC              <  SRR4302533.2311885/94‑1 (MQ=60)
                                                                                  CGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACT             >  SRR4302533.5833258/1‑94 (MQ=60)
                                                                                  CGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACT             >  SRR4302533.1736345/1‑94 (MQ=60)
                                                                                  CGTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACT             <  SRR4302533.2159136/94‑1 (MQ=60)
                                                                                   GTAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTG            >  SRR4302533.2912865/1‑94 (MQ=60)
                                                                                    TAGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGA           >  SRR4302533.1924226/1‑94 (MQ=60)
                                                                                     AGAAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGAT          <  SRR4302533.136951/94‑1 (MQ=60)
                                                                                       AAATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGA        <  SRR4302533.5095486/94‑1 (MQ=60)
                                                                                        AAGCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCTGATAACCCATACGGGTGGCGATTTCACTGATGAT       <  SRR4302533.1001609/94‑1 (MQ=60)
                                                                                         ATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATT      <  SRR4302533.3352055/94‑1 (MQ=60)
                                                                                         ATCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATT      <  SRR4302533.960618/94‑1 (MQ=60)
                                                                                          TCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTT     <  SRR4302533.540912/94‑1 (MQ=60)
                                                                                          TCCAGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTT     >  SRR4302533.4713944/1‑94 (MQ=60)
                                                                                             AGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTTCC  >  SRR4302533.5899552/1‑94 (MQ=60)
                                                                                             AGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTGCC  >  SRR4302533.2252982/1‑94 (MQ=60)
                                                                                             |                                                                                             
GAAGTCCCCTGATCGGCATCTGAAGTATCGCGGCAAGGCCACTGAATGAAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTGCC  >  NC_000913/4295324‑4295510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑