Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,370,019 G→A D473N (GAC→AAC)  mopA → GroEL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,370,0190GA93.9% 1433.5 / 37.5 443D473N (GAC→AAC) mopAGroEL
Reads supporting (aligned to +/- strand):  ref base G (9/18);  new base A (186/230);  total (195/248)
Fisher's exact test for biased strand distribution p-value = 3.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

Only 100 of 477 total aligned reads displayed.

GCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCT  >  NC_000913/4369929‑4370108
                                                                                          |                                                                                         
 cACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACg                                                                                        >  2:9703170/1‑93 (MQ=255)
 cACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACg                                                                                        <  2:2159129/93‑1 (MQ=255)
    tGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGGAACGGCa                                                                                     >  1:7297337/1‑93 (MQ=255)
    tGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCa                                                                                     >  2:4506398/1‑93 (MQ=255)
    tGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCa                                                                                     >  2:3212364/1‑93 (MQ=255)
         gCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTAc                                                                                <  2:80588/93‑1 (MQ=255)
         gCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTAc                                                                                >  2:6658082/1‑93 (MQ=255)
         gCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTAc                                                                                <  1:7602889/93‑1 (MQ=255)
         gCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTAc                                                                                <  2:9096255/93‑1 (MQ=255)
          cAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACg                                                                               <  2:3792631/93‑1 (MQ=255)
           aaTGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACgg                                                                              >  1:2319312/1‑93 (MQ=255)
           aaTGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACgg                                                                              <  2:2948370/93‑1 (MQ=255)
           aaTGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGGTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACgg                                                                              <  1:391007/93‑1 (MQ=255)
            aTGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGt                                                                             <  1:9553397/93‑1 (MQ=255)
            aTGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGt                                                                             >  1:4867531/1‑93 (MQ=255)
               gAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTaa                                                                          >  1:6405515/1‑92 (MQ=255)
                aaGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGGTACa                                                                         >  2:7252984/1‑93 (MQ=255)
                 aGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACaa                                                                        <  1:2961203/93‑1 (MQ=255)
                 aGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACaa                                                                        <  1:8744271/93‑1 (MQ=255)
                 aGCGCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACaa                                                                        <  1:6224008/93‑1 (MQ=255)
                  gCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAAc                                                                       <  1:4638564/93‑1 (MQ=255)
                    tCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACgc                                                                     <  2:9451983/93‑1 (MQ=255)
                     ccGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACgca                                                                    >  2:6103788/1‑93 (MQ=255)
                      cGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAA‑GCCGCGACGGCAACTTCGGTTACAAcgccgc                                                                  >  2:4921662/1‑93 (MQ=255)
                      cGCTGCGTCAGAGCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTGACAACgcag                                                                   >  2:8979000/1‑93 (MQ=255)
                       gCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAATgcagc                                                                  >  2:2001352/1‑93 (MQ=255)
                         tGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCaa                                                                <  1:4371207/93‑1 (MQ=255)
                          gCGTCAGATCGTATTGAACTGCGGCGAAGACCCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAAc                                                               <  2:3421277/93‑1 (MQ=255)
                          gCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAAc                                                               >  1:9927086/1‑93 (MQ=255)
                             tCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCga                                                            >  2:3042562/1‑93 (MQ=255)
                               aGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCgaag                                                          >  2:6377117/1‑93 (MQ=255)
                               aGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCgaag                                                          >  1:6634248/1‑93 (MQ=255)
                                gATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCgaaga                                                         >  1:5817554/1‑93 (MQ=255)
                                gATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCgaaga                                                         <  2:8173567/93‑1 (MQ=255)
                                gATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCgaaga                                                         <  1:5059309/93‑1 (MQ=255)
                                gATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCgaaga                                                         >  2:6522479/1‑93 (MQ=255)
                                  tCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGGAt                                                       >  2:1458393/1‑93 (MQ=255)
                                  tCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAAt                                                       <  2:8716718/93‑1 (MQ=255)
                                  tCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAAt                                                       <  2:5843389/93‑1 (MQ=255)
                                   cGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAGAATa                                                      <  2:1785544/93‑1 (MQ=255)
                                   cGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATa                                                      <  1:4014684/93‑1 (MQ=255)
                                   cGTATTGAACTGCAGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATa                                                      <  1:7125413/93‑1 (MQ=255)
                                   cGTACTGACCTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATa                                                      <  2:3840/93‑1 (MQ=255)
                                    gTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATAc                                                     >  1:5150956/1‑93 (MQ=255)
                                    gTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCAGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATAc                                                     <  1:1980099/93‑1 (MQ=255)
                                     tATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCACCCGACGAATACg                                                    >  1:2703823/1‑93 (MQ=255)
                                     tATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACg                                                    >  1:93502/1‑93 (MQ=255)
                                      aTTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACgg                                                   >  1:6948334/1‑93 (MQ=255)
                                      aTTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACgg                                                   <  1:7877252/93‑1 (MQ=255)
                                       ttGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGc                                                  <  2:6250340/93‑1 (MQ=255)
                                        tGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCa                                                 >  2:4171114/1‑93 (MQ=255)
                                          aaCTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAAc                                               <  2:8850153/93‑1 (MQ=255)
                                          aaCTGCGGCGAAGAACCGTCTGTTGTTGCTAACAACGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAAc                                               <  2:7165397/93‑1 (MQ=255)
                                           aCTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAGCGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACa                                              <  2:2774548/93‑1 (MQ=255)
                                           aCTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACa                                              >  2:9188946/1‑93 (MQ=255)
                                              gcggcgAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGa                                           <  1:7498217/93‑1 (MQ=255)
                                                 gcgAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCg                                        >  1:10008092/1‑93 (MQ=255)
                                                   gaagaaCCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGAc                                      >  2:2582061/1‑93 (MQ=255)
                                                     agaaCCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACAt                                    <  2:4048085/93‑1 (MQ=255)
                                                      gaaCCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATg                                   >  2:10029769/1‑93 (MQ=255)
                                                       aaCCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATgg                                  <  2:9598131/93‑1 (MQ=255)
                                                         ccGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGCCATGGGt                                >  2:8531056/1‑93 (MQ=255)
                                                           gTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTAt                              >  1:2052180/1‑93 (MQ=255)
                                                            tCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATc                             <  2:469992/93‑1 (MQ=255)
                                                            tCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATc                             >  1:3473348/1‑93 (MQ=255)
                                                            tCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACGACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATc                             <  1:8563901/93‑1 (MQ=255)
                                                             cTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATcc                            <  1:8852506/93‑1 (MQ=255)
                                                              tgttgtTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCt                           <  2:7493223/93‑1 (MQ=255)
                                                              tgttgtTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCt                           <  2:70642/93‑1 (MQ=255)
                                                              tgttgtTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATAGACATGGGTATccc                           >  1:4258393/1‑92 (MQ=255)
                                                                ttgttgCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTgg                         <  2:8017060/93‑1 (MQ=255)
                                                                    tgCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATcc                     <  1:9715240/93‑1 (MQ=255)
                                                                      cTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATccaa                   <  2:826488/93‑1 (MQ=255)
                                                                      cTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATccaa                   <  1:3377038/93‑1 (MQ=255)
                                                                      cTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATAAGGCAACATGATCGACATGGGCATCCTGGATccaa                   >  1:2174579/1‑93 (MQ=255)
                                                                        aaCGCCGTTAAAGGCGGCAACGGCAACGACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATccaacc                 <  1:1887036/93‑1 (MQ=255)
                                                                        aaCACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCAAAGAATACGGCAACATGATCGACATGGGTATCCTGGATccaacc                 >  2:644481/1‑93 (MQ=255)
                                                                          cacCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATccaaccaa               <  1:1524878/93‑1 (MQ=255)
                                                                            ccGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAg             <  1:6768605/93‑1 (MQ=255)
                                                                            ccGTGAAAGGCGGCAAGTGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAg             <  1:8683473/93‑1 (MQ=255)
                                                                             cGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGt            <  2:8456352/93‑1 (MQ=255)
                                                                              gTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTa           >  1:5529036/1‑93 (MQ=255)
                                                                              gTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTa           >  1:9009925/1‑93 (MQ=255)
                                                                               ttAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTaa          <  1:8406965/93‑1 (MQ=255)
                                                                               ttAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGaaa          >  1:3254673/1‑93 (MQ=255)
                                                                               ttAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTaa          >  2:1196954/1‑93 (MQ=255)
                                                                               ttAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTaa          >  1:5728611/1‑93 (MQ=255)
                                                                               ttAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTaa          <  1:1095636/93‑1 (MQ=255)
                                                                                tAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTAGATCCAACCAAAGTAAc         >  2:5314433/1‑93 (MQ=255)
                                                                                 aaaGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATTGGTATCCTGGATCCAACCAAAGTAACt        <  1:5969350/93‑1 (MQ=255)
                                                                                 aaaGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACt        <  2:6750101/93‑1 (MQ=255)
                                                                                  aaGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTc       <  1:6596112/93‑1 (MQ=255)
                                                                                  aaGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTc       <  1:2545210/93‑1 (MQ=255)
                                                                                   aGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATGCTGGATCCAACCACAGTAACTCg      >  2:5534799/1‑93 (MQ=255)
                                                                                   aGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACAAGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCg      <  1:5220329/93‑1 (MQ=255)
                                                                                    ggcggcAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGt     >  2:4276235/1‑93 (MQ=255)
                                                                                    ggcggcAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGt     <  2:2233915/93‑1 (MQ=255)
                                                                                    ggcggcAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGt     >  1:1206274/1‑93 (MQ=255)
                                                                                     gcggcaACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGtt    >  2:7841909/1‑93 (MQ=255)
                                                                                       ggcaacggcaacTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTtct  <  2:5701599/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCT  >  NC_000913/4369929‑4370108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 254 total aligned reads displayed.
GTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTC  >  NC_000913/4369926‑4370112
                                                                                             |                                                                                             
GTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCA                                                                                               <  SRR4302540.9101239/94‑1 (MQ=60)
 TGGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAA                                                                                              <  SRR4302540.9125173/94‑1 (MQ=60)
  TGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAAC                                                                                             >  SRR4302540.1692899/1‑94 (MQ=60)
    CACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGG                                                                                           <  SRR4302540.6220435/94‑1 (MQ=60)
     ACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGC                                                                                          >  SRR4302540.13637/1‑94 (MQ=60)
      CTGCGTGCAAGCGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCA                                                                                         <  SRR4302540.9579626/94‑1 (MQ=60)
          GTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTA                                                                                     >  SRR4302540.7968993/1‑94 (MQ=60)
          GTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTA                                                                                     >  SRR4302540.5130735/1‑94 (MQ=60)
           TGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTAC                                                                                    >  SRR4302540.2446922/1‑94 (MQ=60)
           TGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTAC                                                                                    >  SRR4302540.1087024/1‑94 (MQ=60)
           TGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTAC                                                                                    >  SRR4302540.3333474/1‑94 (MQ=60)
           TGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTAC                                                                                    >  SRR4302540.6987507/1‑94 (MQ=60)
            GCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACG                                                                                   <  SRR4302540.7688316/94‑1 (MQ=60)
            GCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACG                                                                                   <  SRR4302540.6827950/94‑1 (MQ=60)
             CAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGG                                                                                  >  SRR4302540.9298631/1‑94 (MQ=60)
             CAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGG                                                                                  <  SRR4302540.1192939/94‑1 (MQ=60)
             CAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGG                                                                                  >  SRR4302540.2344278/1‑94 (MQ=60)
             CAATGGAAGCTACGCTGCGTCAGATCGTATTGAACTGCGGAGAAGAACCGTCTGTTGTTGATAACACCGATAAAGGCGGCGACGGCAACTACGG                                                                                  >  SRR4302540.6297164/1‑94 (MQ=60)
              AATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGGTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGT                                                                                 <  SRR4302540.395049/94‑1 (MQ=60)
               ATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTT                                                                                >  SRR4302540.3362579/1‑94 (MQ=60)
               ATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTT                                                                                <  SRR4302540.9666391/94‑1 (MQ=60)
                TGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTA                                                                               <  SRR4302540.7512929/94‑1 (MQ=60)
                 GGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTAC                                                                              >  SRR4302540.4043368/1‑94 (MQ=60)
                 GGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTAA                                                                              >  SRR4302540.1002974/1‑94 (MQ=60)
                 GGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTAA                                                                              >  SRR4302540.6476812/1‑94 (MQ=60)
                  GAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACA                                                                             >  SRR4302540.5541218/1‑94 (MQ=60)
                   AAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAA                                                                            <  SRR4302540.966821/94‑1 (MQ=60)
                   AAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAA                                                                            <  SRR4302540.2785467/94‑1 (MQ=60)
                    AGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAAC                                                                           <  SRR4302540.2993852/94‑1 (MQ=60)
                    AGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAAC                                                                           <  SRR4302540.8845759/94‑1 (MQ=60)
                    AGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAAC                                                                           <  SRR4302540.36099/94‑1 (MQ=60)
                    AGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAAC                                                                           <  SRR4302540.8173015/94‑1 (MQ=60)
                    AGCGCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAAC                                                                           <  SRR4302540.6293233/94‑1 (MQ=60)
                      CTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTACAGGCGGCAACGGCAAATACGGTTACAACGC                                                                         >  SRR4302540.5849401/1‑94 (MQ=60)
                      CTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGC                                                                         <  SRR4302540.6576556/94‑1 (MQ=60)
                      CTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGC                                                                         <  SRR4302540.6576557/94‑1 (MQ=60)
                        CCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAG                                                                       <  SRR4302540.5289797/94‑1 (MQ=60)
                        CCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAG                                                                       >  SRR4302540.9698333/1‑94 (MQ=60)
                        CCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAG                                                                       <  SRR4302540.6675286/94‑1 (MQ=60)
                         CGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGC                                                                      >  SRR4302540.5139510/1‑94 (MQ=60)
                          GCTGCGTCAGATCGTATTGACCTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTCACGGCGACGACGGCAACTATGGCTACAAAGCAGCA                                                                     >  SRR4302540.7652676/1‑94 (MQ=60)
                          GCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCA                                                                     >  SRR4302540.6246317/1‑94 (MQ=60)
                          GCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCA                                                                     >  SRR4302540.1171096/1‑94 (MQ=60)
                          GCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCA                                                                     >  SRR4302540.2034998/1‑94 (MQ=60)
                            TGCGTCAGATCGTATTGAACTGCGGCGAAGAGCCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCTGCAAC                                                                   <  SRR4302540.6780171/94‑1 (MQ=60)
                            TGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAAC                                                                   <  SRR4302540.4418689/94‑1 (MQ=60)
                            TGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAAC                                                                   >  SRR4302540.10042979/1‑94 (MQ=60)
                            TGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAAC                                                                   <  SRR4302540.2240180/94‑1 (MQ=60)
                            TGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAAC                                                                   <  SRR4302540.8505991/94‑1 (MQ=60)
                             ccggcaagtgctgtttAACTGGGGCGCAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACC                                                                  <  SRR4302540.3283344/78‑1 (MQ=60)
                             GCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACC                                                                  <  SRR4302540.7194223/94‑1 (MQ=60)
                             GCGTCAGATCGTATTCAACTGCGGCGAAGATCCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACC                                                                  <  SRR4302540.10066983/94‑1 (MQ=60)
                              CGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCG                                                                 <  SRR4302540.1957738/94‑1 (MQ=60)
                               GTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGA                                                                >  SRR4302540.6707077/1‑94 (MQ=60)
                               GTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGA                                                                >  SRR4302540.8773182/1‑94 (MQ=60)
                               GTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGA                                                                >  SRR4302540.7373541/1‑94 (MQ=60)
                               GTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGA                                                                <  SRR4302540.7681415/94‑1 (MQ=60)
                               GTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGA                                                                <  SRR4302540.7681416/94‑1 (MQ=60)
                                 taggTCGTATTTAACTGCTGGGAAGAACCGTCGTGTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAG                                                              <  SRR4302540.5998296/90‑1 (MQ=60)
                                 CAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAG                                                              >  SRR4302540.6707671/1‑94 (MQ=60)
                                 CAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAG                                                              >  SRR4302540.2395072/1‑94 (MQ=60)
                                 CAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAA                                                              >  SRR4302540.4354716/1‑94 (MQ=60)
                                  AGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGA                                                             >  SRR4302540.5881864/1‑94 (MQ=60)
                                  AGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGA                                                             <  SRR4302540.4566809/94‑1 (MQ=60)
                                  AGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGA                                                             <  SRR4302540.6935704/94‑1 (MQ=60)
                                  AGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGA                                                             <  SRR4302540.9947450/94‑1 (MQ=60)
                                  AGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGA                                                             <  SRR4302540.1451545/94‑1 (MQ=60)
                                   GATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAGAA                                                            >  SRR4302540.2288855/1‑94 (MQ=60)
                                   GATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAA                                                            <  SRR4302540.9772717/94‑1 (MQ=60)
                                   GATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAA                                                            <  SRR4302540.5259154/94‑1 (MQ=60)
                                   GATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAA                                                            <  SRR4302540.5110739/94‑1 (MQ=60)
                                    ATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAAT                                                           >  SRR4302540.2172485/1‑94 (MQ=60)
                                    ATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAAT                                                           <  SRR4302540.5464447/94‑1 (MQ=60)
                                    ATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAAT                                                           <  SRR4302540.9899023/94‑1 (MQ=60)
                                    ATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAAT                                                           <  SRR4302540.8557904/94‑1 (MQ=60)
                                    ATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAAT                                                           <  SRR4302540.9495613/94‑1 (MQ=60)
                                     TCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATA                                                          <  SRR4302540.6801433/94‑1 (MQ=60)
                                      CGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATAC                                                         <  SRR4302540.4057927/94‑1 (MQ=60)
                                      CGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATAC                                                         >  SRR4302540.8093369/1‑94 (MQ=60)
                                      CGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATAC                                                         <  SRR4302540.3891671/94‑1 (MQ=60)
                                      CGTATTGAACTGCAGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATAC                                                         <  SRR4302540.7204770/94‑1 (MQ=60)
                                       GTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCACCCGACGAATACG                                                        >  SRR4302540.2732991/1‑94 (MQ=60)
                                       GTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACG                                                        <  SRR4302540.7703438/94‑1 (MQ=60)
                                       GTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACG                                                        <  SRR4302540.577458/94‑1 (MQ=60)
                                       GTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCAGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACG                                                        <  SRR4302540.2001578/94‑1 (MQ=60)
                                         ATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGC                                                      <  SRR4302540.5042036/94‑1 (MQ=60)
                                         ATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGC                                                      <  SRR4302540.7969256/94‑1 (MQ=60)
                                            GAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGTCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAAC                                                   <  SRR4302540.8796501/94‑1 (MQ=60)
                                            GAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAAC                                                   <  SRR4302540.9397619/94‑1 (MQ=60)
                                              ACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACAT                                                 >  SRR4302540.2190723/1‑94 (MQ=60)
                                              ACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACAT                                                 <  SRR4302540.7876082/94‑1 (MQ=60)
                                               CTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATG                                                >  SRR4302540.1993834/1‑94 (MQ=60)
                                                 GCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGAT                                              >  SRR4302540.8820545/1‑94 (MQ=60)
                                                 GCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGAT                                              <  SRR4302540.5885196/94‑1 (MQ=60)
                                                 GCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGAT                                              <  SRR4302540.7581995/94‑1 (MQ=60)
                                                  CGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATC                                             <  SRR4302540.7028650/94‑1 (MQ=60)
                                                  CGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATC                                             <  SRR4302540.4277450/94‑1 (MQ=60)
                                                  CGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATC                                             <  SRR4302540.4846149/94‑1 (MQ=60)
                                                  CGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATC                                             <  SRR4302540.4933360/94‑1 (MQ=60)
                                                   GGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCG                                            >  SRR4302540.10124644/1‑94 (MQ=60)
                                                   GGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCG                                            <  SRR4302540.8996897/94‑1 (MQ=60)
                                                   GGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCG                                            >  SRR4302540.6119370/1‑94 (MQ=60)
                                                    GCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGA                                           <  SRR4302540.5869745/94‑1 (MQ=60)
                                                     CGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGAC                                          >  SRR4302540.9496789/1‑94 (MQ=60)
                                                     CGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGAC                                          <  SRR4302540.8859285/94‑1 (MQ=60)
                                                     CGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGAC                                          <  SRR4302540.4265825/94‑1 (MQ=60)
                                                     CGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGAC                                          >  SRR4302540.2317385/1‑94 (MQ=60)
                                                     CGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGAC                                          <  SRR4302540.2603810/94‑1 (MQ=60)
                                                      GAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACA                                         >  SRR4302540.1044207/1‑94 (MQ=60)
                                                       AAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACAT                                        <  SRR4302540.4057438/94‑1 (MQ=60)
                                                       AAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACAT                                        >  SRR4302540.8769365/1‑94 (MQ=60)
                                                       AAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACAT                                        <  SRR4302540.42206/94‑1 (MQ=60)
                                                       AAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACAT                                        <  SRR4302540.6443028/94‑1 (MQ=60)
                                                       AAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACAT                                        <  SRR4302540.330902/94‑1 (MQ=60)
                                                       AAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACAT                                        <  SRR4302540.4555238/94‑1 (MQ=60)
                                                        AGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATG                                       <  SRR4302540.4319137/94‑1 (MQ=60)
                                                        AGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATG                                       >  SRR4302540.6303709/1‑94 (MQ=60)
                                                        AGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATG                                       >  SRR4302540.7597953/1‑94 (MQ=60)
                                                         GAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGG                                      <  SRR4302540.9284595/94‑1 (MQ=60)
                                                           ACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGT                                    <  SRR4302540.4900646/94‑1 (MQ=60)
                                                            CCGTCTGTTGTTGCTACCACCGTTGAAGGCGGCAACGGCAACTACGGTTACAACGCAGCACCCGAAGAATACGGCAACATGATCGACATGGGTA                                   <  SRR4302540.9040309/94‑1 (MQ=60)
                                                            CCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTA                                   <  SRR4302540.8726298/94‑1 (MQ=60)
                                                            CCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTA                                   >  SRR4302540.3438917/1‑94 (MQ=60)
                                                             CGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTAT                                  >  SRR4302540.2074273/1‑94 (MQ=60)
                                                             CGTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTAT                                  >  SRR4302540.7134472/1‑94 (MQ=60)
                                                              GTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATC                                 >  SRR4302540.6999716/1‑94 (MQ=60)
                                                              GTCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATC                                 >  SRR4302540.3511164/1‑94 (MQ=60)
                                                               TCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCC                                >  SRR4302540.2740738/1‑94 (MQ=60)
                                                               TCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCC                                <  SRR4302540.5676021/94‑1 (MQ=60)
                                                               TCTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACGACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCC                                <  SRR4302540.8663354/94‑1 (MQ=60)
                                                                CTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCT                               >  SRR4302540.650317/1‑94 (MQ=60)
                                                                CTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCT                               >  SRR4302540.283168/1‑94 (MQ=60)
                                                                CTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCT                               <  SRR4302540.8954915/94‑1 (MQ=60)
                                                                CTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCT                               <  SRR4302540.4859780/94‑1 (MQ=60)
                                                                CTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCT                               <  SRR4302540.1258676/94‑1 (MQ=60)
                                                                CTGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATAGACATGGGTATCCC                               >  SRR4302540.4304718/1‑94 (MQ=60)
                                                                 tgttttttcgtaccccggTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTG                              <  SRR4302540.3521111/76‑1 (MQ=60)
                                                                 TGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTG                              >  SRR4302540.2130394/1‑94 (MQ=60)
                                                                 TGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTG                              >  SRR4302540.1113612/1‑94 (MQ=60)
                                                                 TGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTG                              >  SRR4302540.5043616/1‑94 (MQ=60)
                                                                 TGTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTG                              <  SRR4302540.5935786/94‑1 (MQ=60)
                                                                  GTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGG                             >  SRR4302540.5822668/1‑94 (MQ=60)
                                                                  GTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGG                             <  SRR4302540.4022668/94‑1 (MQ=60)
                                                                  GTTGTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGG                             >  SRR4302540.5269334/1‑94 (MQ=60)
                                                                     GTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATC                          >  SRR4302540.2058180/1‑94 (MQ=60)
                                                                     GTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATC                          >  SRR4302540.1710813/1‑94 (MQ=60)
                                                                     GTTGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATC                          <  SRR4302540.4862446/94‑1 (MQ=60)
                                                                      TTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCC                         <  SRR4302540.3475884/94‑1 (MQ=60)
                                                                       TGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCA                        <  SRR4302540.9829393/94‑1 (MQ=60)
                                                                       TGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCA                        >  SRR4302540.7206701/1‑94 (MQ=60)
                                                                       TGCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCA                        >  SRR4302540.7206700/1‑94 (MQ=60)
                                                                        GCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAC                       >  SRR4302540.5766115/1‑94 (MQ=60)
                                                                        GCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAA                       <  SRR4302540.3128200/94‑1 (MQ=60)
                                                                        GCTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATAAGGCAACATGATCGACATGGGCATCCTGGATCCAA                       >  SRR4302540.2198032/1‑94 (MQ=60)
                                                                         CTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAAC                      >  SRR4302540.3526068/1‑94 (MQ=60)
                                                                         CTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAAC                      >  SRR4302540.6956549/1‑94 (MQ=60)
                                                                         CTAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAAC                      <  SRR4302540.3413928/94‑1 (MQ=60)
                                                                          TAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACC                     <  SRR4302540.4635684/94‑1 (MQ=60)
                                                                          TAACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACC                     <  SRR4302540.2009800/94‑1 (MQ=60)
                                                                           AACGCCGTTAAAGGCGGCAACGGCAACGACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCA                    <  SRR4302540.1907696/94‑1 (MQ=60)
                                                                           AACACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCA                    >  SRR4302540.4469981/1‑94 (MQ=60)
                                                                             CACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAA                  <  SRR4302540.1541761/94‑1 (MQ=60)
                                                                             CACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAA                  >  SRR4302540.525628/1‑94 (MQ=60)
                                                                             CACCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCACCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAA                  >  SRR4302540.525627/1‑94 (MQ=60)
                                                                               CCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGT                <  SRR4302540.8784313/94‑1 (MQ=60)
                                                                               CCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGA                >  SRR4302540.8865521/1‑94 (MQ=60)
                                                                               CCGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAAGACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGT                >  SRR4302540.5705351/1‑94 (MQ=60)
                                                                               CCGTTAAAGGCGGCAACGGCAACGACGGTTACAACGCAGCAACCGACGAATACGGCAACATGATCGACATGGGCATCCTGGATCCAACCAAAGA                >  SRR4302540.5801712/1‑94 (MQ=60)
                                                                               CCGTGAAAGGCGGCAAGTGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGT                <  SRR4302540.8784312/94‑1 (MQ=60)
                                                                                CGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTA               >  SRR4302540.9118014/1‑94 (MQ=60)
                                                                                CGTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTA               >  SRR4302540.5589933/1‑94 (MQ=60)
                                                                                 GTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAA              >  SRR4302540.3100957/1‑94 (MQ=60)
                                                                                 GTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAA              <  SRR4302540.888270/94‑1 (MQ=60)
                                                                                 GTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAA              >  SRR4302540.7494261/1‑94 (MQ=60)
                                                                                 GTTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGAAA              >  SRR4302540.3290151/1‑94 (MQ=60)
                                                                                  tgaacGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAAC             <  SRR4302540.2673693/89‑1 (MQ=60)
                                                                                  TTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAAC             <  SRR4302540.8504544/94‑1 (MQ=60)
                                                                                  TTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAAC             >  SRR4302540.5143767/1‑94 (MQ=60)
                                                                                  TTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAAC             <  SRR4302540.1106900/94‑1 (MQ=60)
                                                                                  TTAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAAC             <  SRR4302540.2673692/94‑1 (MQ=60)
                                                                                  TTAAAGGCGGCAACGCGAACTACGATTGCACCGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCACCCAAAGTAAC             <  SRR4302540.1750172/94‑1 (MQ=60)
                                                                                   TAAAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACT            >  SRR4302540.5350894/1‑94 (MQ=60)
                                                                                     AAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCG          <  SRR4302540.2572641/94‑1 (MQ=60)
                                                                                     AAGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCG          >  SRR4302540.6704204/1‑94 (MQ=60)
                                                                                      AGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGT         >  SRR4302540.7436416/1‑94 (MQ=60)
                                                                                      AGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGT         >  SRR4302540.1218657/1‑94 (MQ=60)
                                                                                      AGGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGT         <  SRR4302540.607631/94‑1 (MQ=60)
                                                                                       GGCGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTT        <  SRR4302540.315777/94‑1 (MQ=60)
                                                                                         CGGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCT      <  SRR4302540.7984835/94‑1 (MQ=60)
                                                                                          GGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTG     >  SRR4302540.7424280/1‑94 (MQ=60)
                                                                                          GGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTG     >  SRR4302540.680611/1‑94 (MQ=60)
                                                                                          GGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTG     >  SRR4302540.8471670/1‑94 (MQ=60)
                                                                                          GGCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTG     >  SRR4302540.6593615/1‑94 (MQ=60)
                                                                                           GCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGC    <  SRR4302540.2579968/94‑1 (MQ=60)
                                                                                           GCAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGC    <  SRR4302540.963603/94‑1 (MQ=60)
                                                                                            CAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCT   <  SRR4302540.562718/94‑1 (MQ=60)
                                                                                            CAACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCT   <  SRR4302540.5504480/94‑1 (MQ=60)
                                                                                             AACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTC  >  SRR4302540.8967437/1‑94 (MQ=60)
                                                                                             AACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTC  <  SRR4302540.839131/94‑1 (MQ=60)
                                                                                             AACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTC  >  SRR4302540.6428031/1‑94 (MQ=60)
                                                                                             |                                                                                             
GTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTC  >  NC_000913/4369926‑4370112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑