Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,372,457 A→G V260A (GTG→GCG)  yjeK ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,372,4570AG99.8% 1676.1 / ‑5.2 516V260A (GTG→GCG) yjeKhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (223/291);  total (223/293)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-01

Only 100 of 547 total aligned reads displayed.

ACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCA  >  NC_000913/4372367‑4372546
                                                                                          |                                                                                         
aCTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTcgca                                                                                         >  1:538794/1‑93 (MQ=255)
aCTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTcgca                                                                                         <  1:8076184/93‑1 (MQ=255)
aCTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTcgca                                                                                         >  1:887986/1‑93 (MQ=255)
  tttATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATc                                                                                       >  1:7276745/1‑93 (MQ=255)
    tATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCAc                                                                                     <  1:3321707/93‑1 (MQ=255)
    tATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCAc                                                                                     <  2:2987942/93‑1 (MQ=255)
      tCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGt                                                                                   <  2:6950943/93‑1 (MQ=255)
       cGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTa                                                                                  <  1:3463189/93‑1 (MQ=255)
         aGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAAc                                                                                <  1:7005171/93‑1 (MQ=255)
            acaTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGa                                                                             <  2:3543742/93‑1 (MQ=255)
             caTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGaa                                                                            <  1:4122718/93‑1 (MQ=255)
             caTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGaa                                                                            <  1:7747686/93‑1 (MQ=255)
             caTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGaa                                                                            <  1:5011938/93‑1 (MQ=255)
             caTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGaa                                                                            <  2:9744025/93‑1 (MQ=255)
             caTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGaa                                                                            <  2:2108286/93‑1 (MQ=255)
              aTGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAAc                                                                           <  1:7501559/93‑1 (MQ=255)
                gCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGc                                                                         <  2:5853745/93‑1 (MQ=255)
                 cAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTTCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGct                                                                        >  2:8951337/1‑93 (MQ=255)
                  aGGTGATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGctc                                                                       <  2:3977363/93‑1 (MQ=255)
                   ggTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGctct                                                                      >  1:2012369/1‑93 (MQ=255)
                   ggTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGctct                                                                      >  1:1587746/1‑93 (MQ=255)
                   ggTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGctcg                                                                      >  2:6769268/1‑92 (MQ=255)
                    gTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTg                                                                     >  2:8281164/1‑93 (MQ=255)
                    gTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTg                                                                     <  2:9310712/93‑1 (MQ=255)
                      aaTACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGt                                                                   >  1:8745062/1‑93 (MQ=255)
                      aaTACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGt                                                                   >  1:1178205/1‑93 (MQ=255)
                       aTACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGtt                                                                  >  2:4162915/1‑93 (MQ=255)
                        tACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTc                                                                 <  1:8547957/93‑1 (MQ=255)
                         aCGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCa                                                                <  1:7944964/93‑1 (MQ=255)
                          cGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAg                                                               <  2:5086277/93‑1 (MQ=255)
                            gCAATACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCa                                                             <  2:2693929/93‑1 (MQ=255)
                              aTTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCaaa                                                           <  2:5614324/93‑1 (MQ=255)
                               ttACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAg                                                          >  1:5241789/1‑93 (MQ=255)
                                tccgccgcCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGt                                                         <  1:1348829/91‑1 (MQ=255)
                                 aCGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTa                                                        <  1:5711872/93‑1 (MQ=255)
                                  cGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGATCGCTCTGGTTCAGCAAAGTaa                                                       <  2:6118707/93‑1 (MQ=255)
                                  cGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTaa                                                       <  1:9803170/93‑1 (MQ=255)
                                  cGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTaa                                                       >  2:7397286/1‑93 (MQ=255)
                                   gccgccATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAAc                                                      <  2:8108905/93‑1 (MQ=255)
                                    ccGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACg                                                     >  2:638973/1‑93 (MQ=255)
                                    ccGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACg                                                     >  2:720140/1‑93 (MQ=255)
                                     cGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGc                                                    >  2:5751951/1‑93 (MQ=255)
                                     cGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGc                                                    >  1:425552/1‑93 (MQ=255)
                                      ggCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGcc                                                   >  2:761597/1‑93 (MQ=255)
                                       gCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGCACGCTCTGGTTCAGCAAAGTAACGCCt                                                  >  2:4850266/1‑93 (MQ=255)
                                       gCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCt                                                  >  1:2806292/1‑93 (MQ=255)
                                       gCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCt                                                  <  2:163137/93‑1 (MQ=255)
                                       gCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCt                                                  <  1:7639489/93‑1 (MQ=255)
                                       gCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCt                                                  <  1:6437985/93‑1 (MQ=255)
                                        cATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTa                                                 <  1:5927385/93‑1 (MQ=255)
                                          tCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAcc                                               <  1:8045079/93‑1 (MQ=255)
                                          tCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAcc                                               <  2:1911276/93‑1 (MQ=255)
                                           cGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAccc                                              <  2:3139047/93‑1 (MQ=255)
                                             aacaacGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCgg                                            >  1:4663046/1‑93 (MQ=255)
                                             aacaacGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAAGGCCTAccccc                                            >  1:9266669/1‑91 (MQ=255)
                                              acaacGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGgc                                           >  1:3167255/1‑93 (MQ=255)
                                               caacGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGgcg                                          >  1:1770389/1‑93 (MQ=255)
                                                 acGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCa                                        >  1:5654047/1‑93 (MQ=255)
                                                   gCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAAc                                      >  1:9021130/1‑93 (MQ=255)
                                                     aTTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACtt                                    <  2:4285586/93‑1 (MQ=255)
                                                      ttACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTa                                   >  2:5507840/1‑93 (MQ=255)
                                                      ttACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTa                                   <  1:6710511/93‑1 (MQ=255)
                                                      ttACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTa                                   >  2:10032646/1‑93 (MQ=255)
                                                      ttACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTa                                   >  2:7794168/1‑93 (MQ=255)
                                                       tACTCAGGTGTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACATAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAg                                  <  2:9867237/93‑1 (MQ=255)
                                                         cTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGcc                                <  2:9183586/93‑1 (MQ=255)
                                                         cTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGcc                                >  1:2399835/1‑93 (MQ=255)
                                                          tCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCa                               >  1:9511158/1‑93 (MQ=255)
                                                          tCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCa                               >  1:7091848/1‑93 (MQ=255)
                                                          tCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCa                               <  2:7693637/93‑1 (MQ=255)
                                                           cAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCAt                              <  1:2498782/93‑1 (MQ=255)
                                                           cAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCAt                              <  1:3779648/93‑1 (MQ=255)
                                                              gTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGc                           >  2:7903059/1‑93 (MQ=255)
                                                               tttGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGcc                          <  1:4046028/93‑1 (MQ=255)
                                                                   ccAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGAc                      >  1:9396834/1‑93 (MQ=255)
                                                                    cAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACg                     <  1:4554961/93‑1 (MQ=255)
                                                                     aGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACgg                    >  2:654330/1‑93 (MQ=255)
                                                                     aGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACgg                    >  2:1304123/1‑93 (MQ=255)
                                                                     aGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACgg                    >  1:2133989/1‑93 (MQ=255)
                                                                      gCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGa                   >  1:10004394/1‑93 (MQ=255)
                                                                      gCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGa                   >  1:3626472/1‑93 (MQ=255)
                                                                       cGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGaa                  <  2:8405526/93‑1 (MQ=255)
                                                                       cGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGaa                  <  2:9407425/93‑1 (MQ=255)
                                                                        gTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAAt                 >  2:5987255/1‑93 (MQ=255)
                                                                         tttGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATg                >  2:2305547/1‑93 (MQ=255)
                                                                          ttGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGt               <  2:3400750/93‑1 (MQ=255)
                                                                           tgggCGGTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGtt              >  2:1523491/4‑93 (MQ=255)
                                                                            gtgCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGttt             >  1:1807879/1‑93 (MQ=255)
                                                                              gCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCa           <  2:7548662/93‑1 (MQ=255)
                                                                               cGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCAt          <  1:2654904/93‑1 (MQ=255)
                                                                                 ttATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCt        <  1:7348444/93‑1 (MQ=255)
                                                                                 ttATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCt        >  2:3771787/1‑93 (MQ=255)
                                                                                 ttATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCt        >  2:8628658/1‑93 (MQ=255)
                                                                                  tATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTa       <  1:4903679/93‑1 (MQ=255)
                                                                                  tATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTa       <  1:2984434/93‑1 (MQ=255)
                                                                                    tCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTAcc     <  1:6873482/93‑1 (MQ=255)
                                                                                    tCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTAcc     <  2:4493118/93‑1 (MQ=255)
                                                                                    tCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTAcc     <  2:1125694/93‑1 (MQ=255)
                                                                                      gTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACATc   >  1:613359/1‑93 (MQ=255)
                                                                                       ttCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCa  <  1:1129413/93‑1 (MQ=255)
                                                                                          |                                                                                         
ACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCA  >  NC_000913/4372367‑4372546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 300 total aligned reads displayed.
TGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGG  >  NC_000913/4372364‑4372550
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TGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCG                                                                                               <  SRR4302540.168639/94‑1 (MQ=60)
TGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCG                                                                                               >  SRR4302540.9350409/1‑94 (MQ=60)
TGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCG                                                                                               <  SRR4302540.6922891/94‑1 (MQ=60)
 GTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGC                                                                                              >  SRR4302540.3614318/1‑94 (MQ=60)
  TACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCA                                                                                             >  SRR4302540.897413/1‑94 (MQ=60)
  TACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCA                                                                                             >  SRR4302540.544625/1‑94 (MQ=60)
  TACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCA                                                                                             <  SRR4302540.880045/94‑1 (MQ=60)
  TACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCA                                                                                             >  SRR4302540.8060650/1‑94 (MQ=60)
   ACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCAT                                                                                            <  SRR4302540.8169905/94‑1 (MQ=60)
   ACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCAT                                                                                            <  SRR4302540.7493273/94‑1 (MQ=60)
   ACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCAT                                                                                            >  SRR4302540.5755238/1‑94 (MQ=60)
   ACTTTATCGAGCACATGCAGGTAACACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCAT                                                                                            <  SRR4302540.9399501/94‑1 (MQ=60)
    CTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATC                                                                                           >  SRR4302540.7357936/1‑94 (MQ=60)
    CTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATC                                                                                           <  SRR4302540.9430683/94‑1 (MQ=60)
     TTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCA                                                                                          >  SRR4302540.1815143/1‑94 (MQ=60)
     TTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCA                                                                                          <  SRR4302540.3320700/94‑1 (MQ=60)
      TTATCGAGCACATGCAGGTAATACGTCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCAC                                                                                         <  SRR4302540.4268095/94‑1 (MQ=60)
      TTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCAC                                                                                         <  SRR4302540.2213024/94‑1 (MQ=60)
      TTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCAC                                                                                         <  SRR4302540.777171/94‑1 (MQ=60)
       TATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACG                                                                                        <  SRR4302540.3358148/94‑1 (MQ=60)
        ATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGT                                                                                       <  SRR4302540.1220956/94‑1 (MQ=60)
         TCGAGCACATGCAGGTAATCCGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTA                                                                                      <  SRR4302540.2830256/94‑1 (MQ=60)
          CGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAA                                                                                     >  SRR4302540.6424847/1‑94 (MQ=60)
          CGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAA                                                                                     <  SRR4302540.3500890/94‑1 (MQ=60)
           GAGCACATGCAGGTAATACGGCATTAGTCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAAC                                                                                    <  SRR4302540.4707756/94‑1 (MQ=60)
            AGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACA                                                                                   <  SRR4302540.7082912/94‑1 (MQ=60)
             GCACATGCAGTTAATACGGCATTACGCCGTCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAG                                                                                  <  SRR4302540.5785995/94‑1 (MQ=60)
             GCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAG                                                                                  <  SRR4302540.2522343/94‑1 (MQ=60)
             GCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGCTTGCCAGCGTGTGTGCGTTATCGTTCGCATCACGTAACAG                                                                                  >  SRR4302540.7648069/1‑94 (MQ=60)
              CACATGCAGGTAATACTGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGA                                                                                 <  SRR4302540.627098/94‑1 (MQ=60)
              CACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCATCGTTTGTGAGTTGTCGTTCGCATCATGTAGCAGA                                                                                 >  SRR4302540.3069138/1‑94 (MQ=60)
               ACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAA                                                                                <  SRR4302540.86484/94‑1 (MQ=60)
                CATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAAC                                                                               <  SRR4302540.8326814/94‑1 (MQ=60)
                CATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAAC                                                                               <  SRR4302540.3885878/94‑1 (MQ=60)
                CATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAAC                                                                               <  SRR4302540.4167243/94‑1 (MQ=60)
                CATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAAC                                                                               <  SRR4302540.8052729/94‑1 (MQ=60)
                CATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAAC                                                                               >  SRR4302540.5128018/1‑94 (MQ=60)
                CATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAAC                                                                               <  SRR4302540.7838148/94‑1 (MQ=60)
                CATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAAC                                                                               <  SRR4302540.5066202/94‑1 (MQ=60)
                CATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAAC                                                                               <  SRR4302540.2030971/94‑1 (MQ=60)
                CATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAAC                                                                               <  SRR4302540.211248/94‑1 (MQ=60)
                CATGCAGGTAAGTCCGCATTACTCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGGTCGCATCACGTAACAGAAC                                                                               <  SRR4302540.7080188/94‑1 (MQ=60)
                 ATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACG                                                                              <  SRR4302540.3765979/94‑1 (MQ=60)
                 ATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACG                                                                              <  SRR4302540.2860393/94‑1 (MQ=60)
                 ATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACG                                                                              <  SRR4302540.5729403/94‑1 (MQ=60)
                 ATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACG                                                                              <  SRR4302540.4140669/94‑1 (MQ=60)
                  TGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGC                                                                             <  SRR4302540.4882964/94‑1 (MQ=60)
                  TGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGC                                                                             >  SRR4302540.5574753/1‑94 (MQ=60)
                  TGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGC                                                                             >  SRR4302540.2378147/1‑94 (MQ=60)
                  TGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCATTCGCATCACGTAACAGCACGC                                                                             >  SRR4302540.6685449/1‑94 (MQ=60)
                   GCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCT                                                                            <  SRR4302540.6332262/94‑1 (MQ=60)
                   GCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCT                                                                            <  SRR4302540.4721620/94‑1 (MQ=60)
                    CAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTC                                                                           <  SRR4302540.685514/94‑1 (MQ=60)
                     AGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCT                                                                          >  SRR4302540.1605091/1‑94 (MQ=60)
                     AGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCT                                                                          >  SRR4302540.6249179/1‑94 (MQ=60)
                     AGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCT                                                                          >  SRR4302540.2034130/1‑94 (MQ=60)
                     AGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCT                                                                          <  SRR4302540.5439027/94‑1 (MQ=60)
                     AGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCT                                                                          <  SRR4302540.2015436/94‑1 (MQ=60)
                      GGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTG                                                                         <  SRR4302540.7597518/94‑1 (MQ=60)
                      GGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTG                                                                         <  SRR4302540.4133474/94‑1 (MQ=60)
                      GGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTG                                                                         >  SRR4302540.7075323/1‑94 (MQ=60)
                       GTAATCCGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGG                                                                        <  SRR4302540.8148359/94‑1 (MQ=60)
                        TAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGT                                                                       >  SRR4302540.8846553/1‑94 (MQ=60)
                        TAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGT                                                                       >  SRR4302540.8846552/1‑94 (MQ=60)
                         AATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTT                                                                      >  SRR4302540.4117148/1‑94 (MQ=60)
                         AATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTT                                                                      <  SRR4302540.3136726/94‑1 (MQ=60)
                         AATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTT                                                                      >  SRR4302540.500273/1‑94 (MQ=60)
                          ATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTC                                                                     >  SRR4302540.9742979/1‑94 (MQ=60)
                          ATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTC                                                                     <  SRR4302540.4659758/94‑1 (MQ=60)
                          ATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTC                                                                     <  SRR4302540.7972077/94‑1 (MQ=60)
                           TACGGCATTACGCCGGCGTCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCA                                                                    >  SRR4302540.8793981/1‑94 (MQ=60)
                           TACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCA                                                                    <  SRR4302540.8647259/94‑1 (MQ=60)
                           TACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCA                                                                    >  SRR4302540.1755142/1‑94 (MQ=60)
                           TACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCA                                                                    <  SRR4302540.1048141/94‑1 (MQ=60)
                            TCGGCATTACGCCGGCATCGAACAACGCATTCCTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAG                                                                   <  SRR4302540.2653785/94‑1 (MQ=60)
                            ACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAG                                                                   <  SRR4302540.5807930/94‑1 (MQ=60)
                            ACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAG                                                                   <  SRR4302540.8037568/94‑1 (MQ=60)
                             CGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGC                                                                  >  SRR4302540.5291410/1‑94 (MQ=60)
                              GGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCA                                                                 >  SRR4302540.1600938/1‑94 (MQ=60)
                              GGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCA                                                                 >  SRR4302540.1092179/1‑94 (MQ=60)
                              GGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCA                                                                 >  SRR4302540.2379432/1‑94 (MQ=60)
                                CATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAA                                                               <  SRR4302540.3981358/94‑1 (MQ=60)
                                 ATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAG                                                              >  SRR4302540.5298966/1‑94 (MQ=60)
                                 ATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAG                                                              <  SRR4302540.3425139/94‑1 (MQ=60)
                                 ATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAG                                                              >  SRR4302540.6012567/1‑94 (MQ=60)
                                 ATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAG                                                              <  SRR4302540.2511914/94‑1 (MQ=60)
                                     CGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAAC                                                          <  SRR4302540.3169465/94‑1 (MQ=60)
                                     CGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAAC                                                          <  SRR4302540.1309769/94‑1 (MQ=60)
                                     CGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAAC                                                          <  SRR4302540.2995333/94‑1 (MQ=60)
                                     CGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAAC                                                          <  SRR4302540.9917929/94‑1 (MQ=60)
                                     CGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAAC                                                          <  SRR4302540.9156495/94‑1 (MQ=60)
                                     CGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAAC                                                          <  SRR4302540.7567946/94‑1 (MQ=60)
                                     CGCCCGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAAC                                                          <  SRR4302540.7567947/94‑1 (MQ=60)
                                       CCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGC                                                        >  SRR4302540.430029/1‑94 (MQ=60)
                                       CCGGCATCGAACAACGCATTACTCAGGTTTGCAAGAGATTGGGCGTTATCGTTCGCATCACGTAACAGACCGCTCTGGTTCAGCAAAGAAACGC                                                        >  SRR4302540.9935916/1‑94 (MQ=60)
                                        CGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCC                                                       <  SRR4302540.9596115/94‑1 (MQ=60)
                                         GGTATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCT                                                      >  SRR4302540.9045282/1‑94 (MQ=60)
                                         GGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCT                                                      <  SRR4302540.4650758/94‑1 (MQ=60)
                                         GGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCT                                                      <  SRR4302540.4406941/94‑1 (MQ=60)
                                         GGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCT                                                      >  SRR4302540.2438962/1‑94 (MQ=60)
                                         GGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCT                                                      >  SRR4302540.2593581/1‑94 (MQ=60)
                                          GCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTA                                                     <  SRR4302540.7725456/94‑1 (MQ=60)
                                           CATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAC                                                    <  SRR4302540.7874502/94‑1 (MQ=60)
                                           CATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAC                                                    >  SRR4302540.6799749/1‑94 (MQ=60)
                                           CATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTAC                                                    <  SRR4302540.2534251/94‑1 (MQ=60)
                                            ATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACC                                                   >  SRR4302540.8885806/1‑94 (MQ=60)
                                            ATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACC                                                   >  SRR4302540.7135684/1‑94 (MQ=60)
                                             TCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCC                                                  <  SRR4302540.1375436/94‑1 (MQ=60)
                                             TCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCC                                                  <  SRR4302540.8138548/94‑1 (MQ=60)
                                             TCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCC                                                  <  SRR4302540.9642940/94‑1 (MQ=60)
                                               GTACAACGCATTACTCAGGTTTGCCAGCGTATGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGGTCAGCAAAGTAACGCCTACCCGG                                                >  SRR4302540.9033367/1‑94 (MQ=60)
                                               GAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAAGGCCTACCCcc                                                >  SRR4302540.9377501/1‑92 (MQ=60)
                                               GAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGAGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAGGTAACGCCTACACGG                                                >  SRR4302540.2653865/1‑94 (MQ=60)
                                                AACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGC                                               >  SRR4302540.8866909/1‑94 (MQ=60)
                                                AACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGC                                               >  SRR4302540.3201691/1‑94 (MQ=60)
                                                 ACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCG                                              >  SRR4302540.1669858/1‑94 (MQ=60)
                                                 ACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGAAAAGTAACGCCTACCCGGCG                                              >  SRR4302540.5850228/1‑94 (MQ=60)
                                                  CAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGC                                             >  SRR4302540.334306/1‑94 (MQ=60)
                                                  CAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGC                                             >  SRR4302540.2867961/1‑94 (MQ=60)
                                                  CAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGGTCAGCAAAGTAACGCCTACCCGGCGC                                             >  SRR4302540.7790340/1‑94 (MQ=60)
                                                   AACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCA                                            >  SRR4302540.5716295/1‑94 (MQ=60)
                                                     CGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAAC                                          >  SRR4302540.7176231/1‑94 (MQ=60)
                                                     CGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAAC                                          <  SRR4302540.9516829/94‑1 (MQ=60)
                                                     CGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAAC                                          >  SRR4302540.7420319/1‑94 (MQ=60)
                                                     CGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAAC                                          >  SRR4302540.9129377/1‑94 (MQ=60)
                                                      GCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACT                                         >  SRR4302540.4447027/1‑94 (MQ=60)
                                                      GCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACT                                         <  SRR4302540.7537080/94‑1 (MQ=60)
                                                       CATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTT                                        <  SRR4302540.10056867/94‑1 (MQ=60)
                                                         TTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAG                                      <  SRR4302540.6784559/94‑1 (MQ=60)
                                                          TACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGC                                     <  SRR4302540.5890503/94‑1 (MQ=60)
                                                           ACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCC                                    >  SRR4302540.2425709/1‑94 (MQ=60)
                                                           ACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCC                                    <  SRR4302540.7182400/94‑1 (MQ=60)
                                                           ACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCC                                    >  SRR4302540.3766193/1‑94 (MQ=60)
                                                            CTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCA                                   >  SRR4302540.9623816/1‑94 (MQ=60)
                                                            CTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCA                                   >  SRR4302540.972468/1‑94 (MQ=60)
                                                            CTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCA                                   >  SRR4302540.534481/1‑94 (MQ=60)
                                                            CTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCA                                   >  SRR4302540.7170761/1‑94 (MQ=60)
                                                            CTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCA                                   <  SRR4302540.180343/94‑1 (MQ=60)
                                                             TCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCAT                                  <  SRR4302540.3218170/94‑1 (MQ=60)
                                                             TCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCAT                                  <  SRR4302540.7389949/94‑1 (MQ=60)
                                                              CAGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCCAAGTAACGCCTACCCGGCGCAACTTAGCCATC                                 >  SRR4302540.1375638/1‑94 (MQ=60)
                                                              CAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATC                                 <  SRR4302540.3820879/94‑1 (MQ=60)
                                                              CAGGTTTGCCAGCGGTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATC                                 <  SRR4302540.3814196/94‑1 (MQ=60)
                                                               AGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCG                                <  SRR4302540.1774142/94‑1 (MQ=60)
                                                               AGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCG                                >  SRR4302540.1145980/1‑94 (MQ=60)
                                                               AGGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCG                                <  SRR4302540.7854477/94‑1 (MQ=60)
                                                                GGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGC                               >  SRR4302540.599462/1‑94 (MQ=60)
                                                                GGTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGC                               >  SRR4302540.274646/1‑94 (MQ=60)
                                                                 GTTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCC                              <  SRR4302540.8150924/94‑1 (MQ=60)
                                                                  TTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCT                             <  SRR4302540.4089655/94‑1 (MQ=60)
                                                                  TTTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCT                             <  SRR4302540.1190448/94‑1 (MQ=60)
                                                                   TTGCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTG                            >  SRR4302540.5017079/1‑94 (MQ=60)
                                                                     GCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGAC                          >  SRR4302540.9508647/1‑94 (MQ=60)
                                                                     GCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGAC                          <  SRR4302540.5634852/94‑1 (MQ=60)
                                                                     GCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGAC                          <  SRR4302540.4100538/94‑1 (MQ=60)
                                                                     GCCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGAC                          <  SRR4302540.498940/94‑1 (MQ=60)
                                                                      CCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACG                         <  SRR4302540.654626/94‑1 (MQ=60)
                                                                      CCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACG                         <  SRR4302540.5178759/94‑1 (MQ=60)
                                                                      CCAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACG                         <  SRR4302540.5455136/94‑1 (MQ=60)
                                                                       CAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGG                        >  SRR4302540.2700991/1‑94 (MQ=60)
                                                                       CAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGG                        <  SRR4302540.4604211/94‑1 (MQ=60)
                                                                       CAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGG                        <  SRR4302540.3556203/94‑1 (MQ=60)
                                                                       CAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGG                        >  SRR4302540.2156897/1‑94 (MQ=60)
                                                                       CAGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGG                        >  SRR4302540.1911426/1‑94 (MQ=60)
                                                                        acgGTTTGTGCGTTATCGGTCGCATCACGTAACAGAACGCTCTGGTGCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGA                       <  SRR4302540.5738234/91‑1 (MQ=60)
                                                                        AGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGA                       >  SRR4302540.3666160/1‑94 (MQ=60)
                                                                        AGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGA                       <  SRR4302540.8401070/94‑1 (MQ=60)
                                                                        AGCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGA                       >  SRR4302540.10120922/1‑94 (MQ=60)
                                                                         GCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGTGCAACTTAGCCATCGCCTGACGGAA                      <  SRR4302540.3496149/94‑1 (MQ=60)
                                                                         GCGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACAGAA                      <  SRR4302540.2096956/94‑1 (MQ=60)
                                                                          CGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAAT                     <  SRR4302540.8137297/94‑1 (MQ=60)
                                                                          CGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAAT                     >  SRR4302540.8190875/1‑94 (MQ=60)
                                                                          CGTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAAT                     >  SRR4302540.2938471/1‑94 (MQ=60)
                                                                           GTTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATG                    >  SRR4302540.7153363/1‑94 (MQ=60)
                                                                            TTTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGT                   >  SRR4302540.7052934/1‑94 (MQ=60)
                                                                             TTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTT                  >  SRR4302540.3522108/1‑94 (MQ=60)
                                                                             TTGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTT                  >  SRR4302540.6554525/1‑94 (MQ=60)
                                                                              TGTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTT                 >  SRR4302540.1827575/1‑94 (MQ=60)
                                                                               GTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTC                <  SRR4302540.1177160/94‑1 (MQ=60)
                                                                               GTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTC                >  SRR4302540.1297951/1‑94 (MQ=60)
                                                                               GTGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTC                <  SRR4302540.9215578/94‑1 (MQ=60)
                                                                                TGCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCA               >  SRR4302540.1165885/1‑94 (MQ=60)
                                                                                 GCGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCAT              <  SRR4302540.7222282/94‑1 (MQ=60)
                                                                                  CGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATC             >  SRR4302540.4824944/1‑94 (MQ=60)
                                                                                  CGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATC             <  SRR4302540.1813162/94‑1 (MQ=60)
                                                                                  CGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATC             <  SRR4302540.7686466/94‑1 (MQ=60)
                                                                                  CGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATC             <  SRR4302540.2683718/94‑1 (MQ=60)
                                                                                  CGTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTAATC             <  SRR4302540.7414877/94‑1 (MQ=60)
                                                                                   GTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCT            <  SRR4302540.4430958/94‑1 (MQ=60)
                                                                                   GTTATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCT            <  SRR4302540.419799/94‑1 (MQ=60)
                                                                                     TATCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTAC          <  SRR4302540.6048782/94‑1 (MQ=60)
                                                                                       TCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCT        <  SRR4302540.9264217/94‑1 (MQ=60)
                                                                                       TCGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCT        <  SRR4302540.6949665/94‑1 (MQ=60)
                                                                                        CGTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTC       <  SRR4302540.7819306/94‑1 (MQ=60)
                                                                                         GTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCA      >  SRR4302540.4880896/1‑94 (MQ=60)
                                                                                         GTTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCA      >  SRR4302540.8853602/1‑94 (MQ=60)
                                                                                          TTCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCAT     <  SRR4302540.1141166/94‑1 (MQ=60)
                                                                                           TCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATT    >  SRR4302540.5924086/1‑94 (MQ=60)
                                                                                           TCGCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATT    <  SRR4302540.3446182/94‑1 (MQ=60)
                                                                                             GCATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGG  >  SRR4302540.9598900/1‑94 (MQ=60)
                                                                                             |                                                                                             
TGTACTTTATCGAGCACATGCAGGTAATACGGCATTACGCCGGCATCGAACAACGCATTACTCAGGTTTGCCAGCGTTTGTGCGTTATCGTTCACATCACGTAACAGAACGCTCTGGTTCAGCAAAGTAACGCCTACCCGGCGCAACTTAGCCATCGCCTGACGGAATGTTTCATCTACCTCATTGG  >  NC_000913/4372364‑4372550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑