Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,392,958 G→A G439S (GGC→AGC)  yjeF → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,392,9580GA99.5% 1550.0 / ‑5.0 423G439S (GGC→AGC) yjeFhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (231/189);  total (233/190)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

Only 100 of 446 total aligned reads displayed.

GCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGC  >  NC_000913/4392868‑4393046
                                                                                          |                                                                                        
gCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCggcagc                                                                                        >  1:8674024/1‑93 (MQ=255)
gCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCggcagc                                                                                        >  1:9782101/1‑93 (MQ=255)
gCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCggcagc                                                                                        <  2:5344447/93‑1 (MQ=255)
 cGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGgcagca                                                                                       <  2:960609/93‑1 (MQ=255)
   gTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATg                                                                                     <  1:1475351/93‑1 (MQ=255)
    tGCTGAAAGGTGCCGGAACGGTGGTCGCCGCCCAGCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATgg                                                                                    <  1:2459112/93‑1 (MQ=255)
    tGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATgg                                                                                    <  2:9226967/93‑1 (MQ=255)
     gCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATggg                                                                                   <  1:6135874/93‑1 (MQ=255)
     gCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATggg                                                                                   >  2:4948229/1‑93 (MQ=255)
       tGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCg                                                                                 >  2:4259691/1‑93 (MQ=255)
       tGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCg                                                                                 <  1:6848739/93‑1 (MQ=255)
        gAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGa                                                                                <  1:4626739/93‑1 (MQ=255)
             gTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCGTTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGc                                                                           >  2:1201881/1‑93 (MQ=255)
                 cGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGctctc                                                                       <  2:7339882/93‑1 (MQ=255)
                  ggAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGctctct                                                                      <  2:2144599/93‑1 (MQ=255)
                    aaCCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTgg                                                                    >  1:9246122/1‑93 (MQ=255)
                     aCCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGt                                                                   <  1:5101258/93‑1 (MQ=255)
                      ccGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGta                                                                  >  2:2706518/1‑93 (MQ=255)
                      ccGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGta                                                                  <  2:1035822/93‑1 (MQ=255)
                         tGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGtatta                                                               <  1:6043206/93‑1 (MQ=255)
                         tGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGtatta                                                               <  2:5908518/93‑1 (MQ=255)
                         tGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGaatta                                                               >  2:8122420/1‑93 (MQ=255)
                          gctCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTTCTCTCTGGtattat                                                              <  2:6394829/91‑1 (MQ=255)
                           gTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTattatt                                                             <  1:6401275/93‑1 (MQ=255)
                             cgccgcCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTgg                                                           <  2:7742542/93‑1 (MQ=255)
                              gccgccCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGgc                                                          <  2:4641351/93‑1 (MQ=255)
                              gccgccCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGgc                                                          <  2:48690/93‑1 (MQ=255)
                              gccgccCATCCTGACGCTTTAGGCATTATTGATCCCGGAAATGCAGGCATGGCGAGCGTCGGCATGGGCGCTGTGTTCTCTGGGATTTTTCgc                                                          >  2:9722260/1‑93 (MQ=255)
                                 gccCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCa                                                       <  2:6800640/93‑1 (MQ=255)
                                 gccCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCa                                                       >  1:1913869/1‑93 (MQ=255)
                                 gccCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCa                                                       <  1:345793/93‑1 (MQ=255)
                                  cccATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCAt                                                      <  1:8300502/93‑1 (MQ=255)
                                    cATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTg                                                    >  1:2632096/1‑93 (MQ=255)
                                    cATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTg                                                    >  1:720667/1‑93 (MQ=255)
                                    cATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTg                                                    <  2:2482991/93‑1 (MQ=255)
                                     aTCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGc                                                   <  1:1037214/93‑1 (MQ=255)
                                      tCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCt                                                  <  1:7151709/93‑1 (MQ=255)
                                      tCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCt                                                  >  2:7858555/1‑93 (MQ=255)
                                      tCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCt                                                  >  2:1609663/1‑93 (MQ=255)
                                       ccTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCtt                                                 <  1:5608974/93‑1 (MQ=255)
                                       ccTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCtt                                                 <  1:5268545/93‑1 (MQ=255)
                                        cTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTg                                                <  1:6590050/93‑1 (MQ=255)
                                        cTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTg                                                >  2:9455108/1‑93 (MQ=255)
                                        cTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTg                                                >  1:8813386/1‑93 (MQ=255)
                                          gACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTCGCGCATTGCTTggg                                              >  1:75507/1‑93 (MQ=255)
                                           aCGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGc                                             <  1:4429482/93‑1 (MQ=255)
                                           aCGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGc                                             >  2:3015107/1‑93 (MQ=255)
                                            cGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCa                                            >  2:4468982/1‑93 (MQ=255)
                                             gCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCaa                                           >  1:430763/1‑93 (MQ=255)
                                               tttAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCaaaa                                         <  2:8974089/93‑1 (MQ=255)
                                               tttAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCaaaa                                         >  1:1073450/1‑93 (MQ=255)
                                               tttAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCaaaa                                         <  2:6631543/93‑1 (MQ=255)
                                                ttAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCaaaaa                                        >  1:5496978/1‑93 (MQ=255)
                                                 tAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTTGGATTATTGGCGCATTGCTTGGGCAAAAAc                                       >  2:5597102/1‑93 (MQ=255)
                                                 tAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAAc                                       <  1:4013858/93‑1 (MQ=255)
                                                 tAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAAc                                       <  1:3816103/93‑1 (MQ=255)
                                                 tAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAAc                                       <  2:9154617/93‑1 (MQ=255)
                                                  aGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACt                                      <  1:7733653/93‑1 (MQ=255)
                                                   ggCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTg                                     >  2:6005933/1‑93 (MQ=255)
                                                     cATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTc                                   <  1:4174575/93‑1 (MQ=255)
                                                      attattGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCg                                  >  2:1262544/1‑93 (MQ=255)
                                                       ttattGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGc                                 >  2:2221953/1‑93 (MQ=255)
                                                        tattGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGcc                                >  2:2881427/1‑93 (MQ=255)
                                                         attGATGCCGGAAATGCAGGCCTGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTCTTGGCGCATGGCTTGGGCAAAAATCGccgccg                               >  1:1368219/1‑93 (MQ=255)
                                                         attGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCg                               >  1:2275935/1‑93 (MQ=255)
                                                         attGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCg                               >  2:4164391/1‑93 (MQ=255)
                                                           tGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTa                             <  2:7186466/93‑1 (MQ=255)
                                                           tGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTa                             <  2:2369032/93‑1 (MQ=255)
                                                            gATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTat                            >  1:3749049/1‑93 (MQ=255)
                                                            gATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTat                            <  2:3875020/93‑1 (MQ=255)
                                                            gATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTat                            <  2:8462494/93‑1 (MQ=255)
                                                            gATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATCGGCGCATTGCTTGGGCAAAAACTGTCGCCGTat                            >  1:8506294/1‑93 (MQ=255)
                                                             aTGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTatg                           >  1:9097344/1‑93 (MQ=255)
                                                              tGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTatga                          <  1:6376732/93‑1 (MQ=255)
                                                              tGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTatga                          <  1:7103403/93‑1 (MQ=255)
                                                               gCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTatgat                         >  2:1272006/1‑93 (MQ=255)
                                                               gCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTatgat                         <  1:5823596/93‑1 (MQ=255)
                                                                  ggAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCa                      >  1:9633083/1‑93 (MQ=255)
                                                                  ggAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCa                      <  2:8577215/93‑1 (MQ=255)
                                                                  ggAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCa                      <  2:8073941/93‑1 (MQ=255)
                                                                   gAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAg                     >  2:1832996/1‑93 (MQ=255)
                                                                   gAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAg                     <  2:1923095/93‑1 (MQ=255)
                                                                     aaTGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGcc                   <  1:2589677/93‑1 (MQ=255)
                                                                       tGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCtg                 <  2:7629858/93‑1 (MQ=255)
                                                                         cAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCtgtg               >  2:8758021/1‑93 (MQ=255)
                                                                          aGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGc              >  1:315255/1‑93 (MQ=255)
                                                                           ggCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCa             >  2:1484131/1‑93 (MQ=255)
                                                                            gCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAg            >  1:8072853/1‑93 (MQ=255)
                                                                            gCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAg            <  2:3571964/93‑1 (MQ=255)
                                                                               tGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAACAACTGTCGCCGTATGATGCAGCCTGTGCAGGcg         >  1:10010942/1‑92 (MQ=255)
                                                                               tGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCt         >  1:3048236/1‑93 (MQ=255)
                                                                               tGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCt         >  2:3661013/1‑93 (MQ=255)
                                                                               tGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCt         <  2:3233848/93‑1 (MQ=255)
                                                                               tGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCt         >  2:1127243/1‑93 (MQ=255)
                                                                                ggCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCtg        >  1:1704570/1‑93 (MQ=255)
                                                                                 gCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCtgt       <  2:8894691/93‑1 (MQ=255)
                                                                                 gCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCtgt       <  1:4734610/93‑1 (MQ=255)
                                                                                 gCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCtgt       <  1:7933856/93‑1 (MQ=255)
                                                                                   gAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTAAGATGCAGCCTGTGCAGGCTgtgt     >  2:6936489/1‑93 (MQ=255)
                                                                                     gcggcaGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTcg   <  2:6222430/93‑1 (MQ=255)
                                                                                          |                                                                                        
GCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGC  >  NC_000913/4392868‑4393046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 227 total aligned reads displayed.
GTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACG  >  NC_000913/4392865‑4393051
                                                                                             |                                                                                             
GTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCA                                                                                               >  SRR4302540.4756158/1‑94 (MQ=60)
GTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCA                                                                                               >  SRR4302540.8675139/1‑94 (MQ=60)
 TAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAG                                                                                              <  SRR4302540.843511/94‑1 (MQ=60)
  AGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGC                                                                                             >  SRR4302540.9896741/1‑94 (MQ=60)
  AGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGC                                                                                             >  SRR4302540.5163350/1‑94 (MQ=60)
  AGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGC                                                                                             >  SRR4302540.4995202/1‑94 (MQ=60)
  AGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGC                                                                                             >  SRR4302540.8774774/1‑94 (MQ=60)
  AGCGGTGCTGAAAGGTGCCGGAACCCTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGC                                                                                             >  SRR4302540.5712474/1‑94 (MQ=60)
    CGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCAT                                                                                           <  SRR4302540.8561180/94‑1 (MQ=60)
    CGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCAT                                                                                           <  SRR4302540.8263224/94‑1 (MQ=60)
    CGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCCAGCGGCAGCAT                                                                                           >  SRR4302540.7545421/1‑94 (MQ=60)
     GGTGCTGAAAGGTGCCGGACCCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATG                                                                                          <  SRR4302540.2058064/94‑1 (MQ=60)
     GGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATG                                                                                          <  SRR4302540.3019022/94‑1 (MQ=60)
     GGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATG                                                                                          >  SRR4302540.5973459/1‑94 (MQ=60)
     GGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATG                                                                                          >  SRR4302540.8094104/1‑94 (MQ=60)
     GGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATG                                                                                          >  SRR4302540.8146352/1‑94 (MQ=60)
     GGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATG                                                                                          >  SRR4302540.3811866/1‑94 (MQ=60)
     GGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATG                                                                                          >  SRR4302540.3811864/1‑94 (MQ=60)
     GGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATGGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATG                                                                                          <  SRR4302540.4330363/94‑1 (MQ=60)
      GTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGG                                                                                         <  SRR4302540.1491482/94‑1 (MQ=60)
      GTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGG                                                                                         <  SRR4302540.1491484/94‑1 (MQ=60)
      GTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGG                                                                                         <  SRR4302540.7230769/94‑1 (MQ=60)
      GTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGG                                                                                         >  SRR4302540.2452325/1‑94 (MQ=60)
       TGCTGAAAGGTGCCGGAACGGTGGTCGCCGCCCAGCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGG                                                                                        <  SRR4302540.2485569/94‑1 (MQ=60)
       GGCTGAAAGGTGCCGGAGCCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGG                                                                                        <  SRR4302540.2485570/94‑1 (MQ=60)
        GCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACTCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGC                                                                                       <  SRR4302540.819350/94‑1 (MQ=60)
        GCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGC                                                                                       >  SRR4302540.3615918/1‑94 (MQ=60)
        GCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGC                                                                                       <  SRR4302540.6204215/94‑1 (MQ=60)
        GCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGC                                                                                       >  SRR4302540.105614/1‑94 (MQ=60)
         CTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCG                                                                                      >  SRR4302540.6187822/1‑94 (MQ=60)
         CTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCG                                                                                      >  SRR4302540.6406488/1‑94 (MQ=60)
           GAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGAT                                                                                    <  SRR4302540.4676307/94‑1 (MQ=60)
           GAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGAT                                                                                    <  SRR4302540.2104373/94‑1 (MQ=60)
            AAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATG                                                                                   <  SRR4302540.372188/94‑1 (MQ=60)
            AAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATG                                                                                   >  SRR4302540.10104796/1‑94 (MQ=60)
            AAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATG                                                                                   >  SRR4302540.10104798/1‑94 (MQ=60)
            AAAGCTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATG                                                                                   <  SRR4302540.372187/94‑1 (MQ=60)
                GTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCT                                                                               >  SRR4302540.3153071/1‑94 (MQ=60)
                 TGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTC                                                                              <  SRR4302540.9107333/94‑1 (MQ=60)
                 TGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTC                                                                              <  SRR4302540.9107334/94‑1 (MQ=60)
                 TGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTC                                                                              <  SRR4302540.9377481/94‑1 (MQ=60)
                  GCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCT                                                                             <  SRR4302540.1393644/94‑1 (MQ=60)
                  GCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCT                                                                             >  SRR4302540.9497844/1‑94 (MQ=60)
                   CCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTC                                                                            >  SRR4302540.6184528/1‑94 (MQ=60)
                    CGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCT                                                                           <  SRR4302540.390390/94‑1 (MQ=60)
                     GGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTG                                                                          >  SRR4302540.9899009/1‑94 (MQ=60)
                     GGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTG                                                                          >  SRR4302540.5561562/1‑94 (MQ=60)
                     GGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTG                                                                          >  SRR4302540.7712108/1‑94 (MQ=60)
                      GAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGG                                                                         >  SRR4302540.4043742/1‑94 (MQ=60)
                      GAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGG                                                                         <  SRR4302540.8350426/94‑1 (MQ=60)
                      GAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGG                                                                         >  SRR4302540.9356833/1‑94 (MQ=60)
                       AACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGT                                                                        >  SRR4302540.6415098/1‑94 (MQ=60)
                       AACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGT                                                                        >  SRR4302540.9207532/1‑94 (MQ=60)
                        ACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTA                                                                       >  SRR4302540.5163086/1‑94 (MQ=60)
                        ACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTA                                                                       >  SRR4302540.6108378/1‑94 (MQ=60)
                        ACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTA                                                                       <  SRR4302540.5156470/94‑1 (MQ=60)
                         CCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTAT                                                                      <  SRR4302540.4460174/94‑1 (MQ=60)
                         CCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTAT                                                                      >  SRR4302540.4441831/1‑94 (MQ=60)
                         CCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTAT                                                                      >  SRR4302540.559953/1‑94 (MQ=60)
                          CGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATT                                                                     >  SRR4302540.5932797/1‑94 (MQ=60)
                          CGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATT                                                                     >  SRR4302540.1114746/1‑94 (MQ=60)
                           GTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTA                                                                    >  SRR4302540.8901056/1‑94 (MQ=60)
                           GTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTA                                                                    <  SRR4302540.6265993/94‑1 (MQ=60)
                           GTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTA                                                                    >  SRR4302540.6627117/1‑94 (MQ=60)
                            TGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTAT                                                                   <  SRR4302540.6110331/94‑1 (MQ=60)
                              GTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTG                                                                 <  SRR4302540.1593601/94‑1 (MQ=60)
                              GTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTG                                                                 <  SRR4302540.6472524/94‑1 (MQ=60)
                                CGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGC                                                               >  SRR4302540.5462792/1‑94 (MQ=60)
                                CGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGC                                                               <  SRR4302540.6569953/94‑1 (MQ=60)
                                CGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGC                                                               <  SRR4302540.7751654/94‑1 (MQ=60)
                                  CCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGC                                                             >  SRR4302540.7970765/1‑94 (MQ=60)
                                  CCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGC                                                             <  SRR4302540.7817813/94‑1 (MQ=60)
                                   CGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCA                                                            >  SRR4302540.1015659/1‑94 (MQ=60)
                                   CGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCA                                                            >  SRR4302540.1934765/1‑94 (MQ=60)
                                   CGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCA                                                            >  SRR4302540.723896/1‑94 (MQ=60)
                                   CGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCA                                                            >  SRR4302540.7156372/1‑94 (MQ=60)
                                    GCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCAT                                                           >  SRR4302540.3362356/1‑94 (MQ=60)
                                    GCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCAT                                                           >  SRR4302540.2821537/1‑94 (MQ=60)
                                    GCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCAT                                                           <  SRR4302540.349313/94‑1 (MQ=60)
                                    GCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCAT                                                           >  SRR4302540.6650733/1‑94 (MQ=60)
                                     CCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATT                                                          <  SRR4302540.8396877/94‑1 (MQ=60)
                                      CCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTG                                                         <  SRR4302540.9853090/94‑1 (MQ=60)
                                      CCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTG                                                         >  SRR4302540.728394/1‑94 (MQ=60)
                                      CCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTG                                                         <  SRR4302540.9304192/94‑1 (MQ=60)
                                      CCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTG                                                         <  SRR4302540.8724095/94‑1 (MQ=60)
                                      CCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTG                                                         >  SRR4302540.2660585/1‑94 (MQ=60)
                                      CCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTG                                                         >  SRR4302540.2660583/1‑94 (MQ=60)
                                       CATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGC                                                        <  SRR4302540.8212184/94‑1 (MQ=60)
                                        ATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCT                                                       <  SRR4302540.1047763/94‑1 (MQ=60)
                                        ATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCT                                                       >  SRR4302540.461302/1‑94 (MQ=60)
                                        ATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCT                                                       <  SRR4302540.5302286/94‑1 (MQ=60)
                                        ATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCT                                                       >  SRR4302540.8894075/1‑94 (MQ=60)
                                        ATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCT                                                       >  SRR4302540.5810550/1‑94 (MQ=60)
                                         TCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTTGCGCATTGCTT                                                      >  SRR4302540.6453692/1‑94 (MQ=60)
                                         TCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTT                                                      <  SRR4302540.8644437/94‑1 (MQ=60)
                                         TCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTT                                                      <  SRR4302540.7231405/94‑1 (MQ=60)
                                          CCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTG                                                     >  SRR4302540.8915289/1‑94 (MQ=60)
                                          CCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTG                                                     <  SRR4302540.5670679/94‑1 (MQ=60)
                                          CCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTG                                                     >  SRR4302540.4229027/1‑94 (MQ=60)
                                           CTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGG                                                    <  SRR4302540.6663042/94‑1 (MQ=60)
                                            TGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGTCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGG                                                   >  SRR4302540.7577694/1‑94 (MQ=60)
                                            TGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGG                                                   >  SRR4302540.3792675/1‑94 (MQ=60)
                                            TGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGG                                                   >  SRR4302540.4063578/1‑94 (MQ=60)
                                            TGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTCGCGCATTGCTTGGG                                                   >  SRR4302540.76290/1‑94 (MQ=60)
                                             GACGCTTTTGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGC                                                  <  SRR4302540.645356/94‑1 (MQ=60)
                                              ACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCA                                                 <  SRR4302540.4477476/94‑1 (MQ=60)
                                              ACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCA                                                 >  SRR4302540.6717372/1‑94 (MQ=60)
                                               CGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAA                                                >  SRR4302540.435317/1‑94 (MQ=60)
                                                GCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAA                                               >  SRR4302540.1620105/1‑94 (MQ=60)
                                                GCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAA                                               >  SRR4302540.2058471/1‑94 (MQ=60)
                                                 CTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCACAA                                              >  SRR4302540.1084411/1‑94 (MQ=60)
                                                 CTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAA                                              >  SRR4302540.1084412/1‑94 (MQ=60)
                                                 CTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAA                                              >  SRR4302540.1801195/1‑94 (MQ=60)
                                                  TTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAA                                             >  SRR4302540.2599419/1‑94 (MQ=60)
                                                  TTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAA                                             >  SRR4302540.5557501/1‑94 (MQ=60)
                                                   TTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAAC                                            <  SRR4302540.4353158/94‑1 (MQ=60)
                                                   TTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAAC                                            >  SRR4302540.8673300/1‑94 (MQ=60)
                                                    TAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACT                                           <  SRR4302540.4057091/94‑1 (MQ=60)
                                                    TAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACT                                           <  SRR4302540.3857655/94‑1 (MQ=60)
                                                    TAGGCATTATTGATGCCGGAAAGGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACT                                           <  SRR4302540.4057092/94‑1 (MQ=60)
                                                       GCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTC                                        <  SRR4302540.1522664/94‑1 (MQ=60)
                                                        CATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCG                                       <  SRR4302540.4219878/94‑1 (MQ=60)
                                                        CATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCG                                       >  SRR4302540.5801424/1‑94 (MQ=60)
                                                         ATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGC                                      >  SRR4302540.788704/1‑94 (MQ=60)
                                                         ATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGC                                      >  SRR4302540.6000102/1‑94 (MQ=60)
                                                         ATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGC                                      >  SRR4302540.2645593/1‑94 (MQ=60)
                                                         ATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCAATGTGCTCTCTGGTATTATTGGCGAATTGCTTGGGCACAAAGTGTCCC                                      >  SRR4302540.7885202/1‑94 (MQ=60)
                                                          TTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCC                                     >  SRR4302540.1020418/1‑94 (MQ=60)
                                                          TTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCC                                     <  SRR4302540.7251253/94‑1 (MQ=60)
                                                           TATTGATGCCGGAAATGCAGGCCTGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTCTTGGCGCATGGCTTGGGCAAAAAtcgccgccg                                    >  SRR4302540.1382706/1‑85 (MQ=60)
                                                           TATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCG                                    >  SRR4302540.2300373/1‑94 (MQ=60)
                                                            ATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGT                                   >  SRR4302540.6838870/1‑94 (MQ=60)
                                                            ATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGT                                   <  SRR4302540.61684/94‑1 (MQ=60)
                                                            ATTGATGCCGGAAATGCAGGCATGGCGAGCCGCAGCATGGGCGATGTGCTCTCTGTTATTATTAGCGCATTGCTTGGGCAAAAACTTTCGCCGT                                   >  SRR4302540.2843051/1‑94 (MQ=60)
                                                             TTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTA                                  >  SRR4302540.7057780/1‑94 (MQ=60)
                                                             TTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTA                                  >  SRR4302540.297874/1‑94 (MQ=60)
                                                              TGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAACACTGTCGCCGTAT                                 >  SRR4302540.4127322/1‑94 (MQ=60)
                                                              TGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTAT                                 <  SRR4302540.8557861/94‑1 (MQ=60)
                                                              TGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTAT                                 <  SRR4302540.3411967/94‑1 (MQ=60)
                                                              TGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTAT                                 >  SRR4302540.3789955/1‑94 (MQ=60)
                                                              TGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTAT                                 >  SRR4302540.2478843/1‑94 (MQ=60)
                                                              TGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATCGGCGCATTGCTTGGGCAAAAACTGTCGCCGTAT                                 >  SRR4302540.8605149/1‑94 (MQ=60)
                                                               GATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATG                                >  SRR4302540.9206651/1‑94 (MQ=60)
                                                               GATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATG                                <  SRR4302540.2448743/94‑1 (MQ=60)
                                                               GATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATG                                <  SRR4302540.1813201/94‑1 (MQ=60)
                                                                ATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGA                               >  SRR4302540.7233418/1‑94 (MQ=60)
                                                                 TGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGAT                              <  SRR4302540.6447727/94‑1 (MQ=60)
                                                                 TGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGAT                              <  SRR4302540.1593422/94‑1 (MQ=60)
                                                                 TGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGAT                              <  SRR4302540.4607252/94‑1 (MQ=60)
                                                                 TGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGAT                              <  SRR4302540.5493476/94‑1 (MQ=60)
                                                                 TGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGAT                              <  SRR4302540.7182437/94‑1 (MQ=60)
                                                                  GCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATG                             <  SRR4302540.5887971/94‑1 (MQ=60)
                                                                  GCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATG                             <  SRR4302540.9187498/94‑1 (MQ=60)
                                                                   CCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGC                            <  SRR4302540.1174542/94‑1 (MQ=60)
                                                                   CCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGC                            >  SRR4302540.7272782/1‑94 (MQ=60)
                                                                    CGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCA                           >  SRR4302540.9746585/1‑94 (MQ=60)
                                                                    CGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCA                           <  SRR4302540.2628859/94‑1 (MQ=60)
                                                                       AAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCC                        >  SRR4302540.4767201/1‑94 (MQ=60)
                                                                        AATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCT                       <  SRR4302540.2617594/94‑1 (MQ=60)
                                                                         ATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGGAGCCTG                      >  SRR4302540.340838/1‑94 (MQ=60)
                                                                         ATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTG                      >  SRR4302540.4952008/1‑94 (MQ=60)
                                                                         ATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTG                      <  SRR4302540.6893480/94‑1 (MQ=60)
                                                                          TGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGT                     >  SRR4302540.10132201/1‑94 (MQ=60)
                                                                            CAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGC                   >  SRR4302540.7821170/1‑94 (MQ=60)
                                                                            CAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGC                   >  SRR4302540.318394/1‑94 (MQ=60)
                                                                             AGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCA                  >  SRR4302540.7217035/1‑94 (MQ=60)
                                                                              GGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTTGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAG                 <  SRR4302540.4933896/94‑1 (MQ=60)
                                                                              GGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAG                 <  SRR4302540.3165983/94‑1 (MQ=60)
                                                                              GGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAG                 >  SRR4302540.6471121/1‑94 (MQ=60)
                                                                              GGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAG                 >  SRR4302540.8166523/1‑94 (MQ=60)
                                                                              GGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAG                 <  SRR4302540.7056633/94‑1 (MQ=60)
                                                                              GGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAG                 >  SRR4302540.5140723/1‑94 (MQ=60)
                                                                               GCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGG                <  SRR4302540.1120790/94‑1 (MQ=60)
                                                                                CATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGC               <  SRR4302540.4688517/94‑1 (MQ=60)
                                                                                 ATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAACAACTGTCGCCGTATGATGCAGCCTGTGCAGGCG              >  SRR4302540.10127510/1‑94 (MQ=60)
                                                                                 ATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCT              >  SRR4302540.5487457/1‑94 (MQ=60)
                                                                                 ATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCT              >  SRR4302540.3081682/1‑94 (MQ=60)
                                                                                 ATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCT              >  SRR4302540.4542707/1‑94 (MQ=60)
                                                                                 ATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCT              >  SRR4302540.4678751/1‑94 (MQ=60)
                                                                                 ATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCT              <  SRR4302540.9328284/94‑1 (MQ=60)
                                                                                  TGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTG             >  SRR4302540.1723005/1‑94 (MQ=60)
                                                                                  TGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTG             <  SRR4302540.7847433/94‑1 (MQ=60)
                                                                                    GCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTG           <  SRR4302540.8026328/94‑1 (MQ=60)
                                                                                     CGAGCGGCAGCATTGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGT          <  SRR4302540.1103781/94‑1 (MQ=60)
                                                                                     CGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGT          <  SRR4302540.8893471/94‑1 (MQ=60)
                                                                                     CGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGT          <  SRR4302540.9473501/94‑1 (MQ=60)
                                                                                     CGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATCGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGT          >  SRR4302540.2498590/1‑94 (MQ=60)
                                                                                      GAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTC         >  SRR4302540.4404339/1‑94 (MQ=60)
                                                                                       AGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAACACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCG        <  SRR4302540.3898950/94‑1 (MQ=60)
                                                                                       AGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCG        <  SRR4302540.9821033/94‑1 (MQ=60)
                                                                                       AGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGAAGCCTGTGCAGGCTGTGTCG        <  SRR4302540.5345733/94‑1 (MQ=60)
                                                                                        GCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGC       >  SRR4302540.7083623/1‑94 (MQ=60)
                                                                                         CGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCG      <  SRR4302540.9810189/94‑1 (MQ=60)
                                                                                          GGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGC     >  SRR4302540.1875122/1‑94 (MQ=60)
                                                                                           GCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCA    >  SRR4302540.7480442/1‑94 (MQ=60)
                                                                                            CAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCAC   <  SRR4302540.6819202/94‑1 (MQ=60)
                                                                                            CAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCAC   <  SRR4302540.7046480/94‑1 (MQ=60)
                                                                                            CAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCAC   >  SRR4302540.2491865/1‑94 (MQ=60)
                                                                                             AGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACG  >  SRR4302540.1982160/1‑94 (MQ=60)
                                                                                             AGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACG  <  SRR4302540.7843469/94‑1 (MQ=60)
                                                                                             |                                                                                             
GTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACG  >  NC_000913/4392865‑4393051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑