Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,392,958	G→A	G439S (GGC→AGC)	yjeF →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,392,958	0	G	A	100.0%	1093.1 / NA	290	G439S (GGC→AGC)	yjeF	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (144/146); total (144/146)

Only 100 of 301 total aligned reads displayed.

GCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCG  >  NC_000913/4392868‑4393047

 cGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGgcagca                                                                                        <  1:4886557/93‑1 (MQ=255)

 cGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGgcagca                                                                                        <  2:4805629/93‑1 (MQ=255)

  ggTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCAt                                                                                       >  2:4745666/1‑93 (MQ=255)

   gTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATg                                                                                      <  1:131969/93‑1 (MQ=255)

   gTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATg                                                                                      <  1:794716/93‑1 (MQ=255)

     gCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATggg                                                                                    >  1:4218659/1‑93 (MQ=255)

      cTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGc                                                                                   <  1:3652948/93‑1 (MQ=255)

      cTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGc                                                                                   >  2:2691541/1‑93 (MQ=255)

       tGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCg                                                                                  >  2:1939224/1‑93 (MQ=255)

        gAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGa                                                                                 >  2:5738208/1‑93 (MQ=255)

        gAAAGGTGCCGGAACCGTCGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGa                                                                                 <  2:3882800/93‑1 (MQ=255)

          aaGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGAtg                                                                               <  1:1627372/93‑1 (MQ=255)

           aGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGAtgt                                                                              <  1:2153712/93‑1 (MQ=255)

           aGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGAtgt                                                                              >  1:3230219/1‑93 (MQ=255)

            ggTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGAtgtg                                                                             >  1:3082104/1‑93 (MQ=255)

               gCCGGAGCCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGctc                                                                          <  2:1490583/93‑1 (MQ=255)

               gCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGctc                                                                          <  1:1177593/93‑1 (MQ=255)

                ccGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGctct                                                                         >  2:2583529/1‑93 (MQ=255)

                 cGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGctctc                                                                        >  2:3454128/1‑93 (MQ=255)

                 cGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGctctc                                                                        <  1:4347016/93‑1 (MQ=255)

                  ggAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGctctct                                                                       <  1:6211997/93‑1 (MQ=255)

                    aaCCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTgg                                                                     >  2:2828758/1‑93 (MQ=255)

                     aCCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGt                                                                    <  2:6203999/93‑1 (MQ=255)

                     aCCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGt                                                                    >  2:4165935/1‑93 (MQ=255)

                     aCCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGt                                                                    <  1:45637/93‑1 (MQ=255)

                     aCCGTGGTCGCCGCCCATCCTAACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGt                                                                    >  2:4010812/1‑93 (MQ=255)

                      ccGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGta                                                                   <  1:2907547/93‑1 (MQ=255)

                      ccGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGta                                                                   >  1:5157082/1‑93 (MQ=255)

                        gTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGtatt                                                                 >  2:6256742/1‑93 (MQ=255)

                        gTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGtatt                                                                 >  2:5912836/1‑93 (MQ=255)

                         tGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGtatta                                                                >  2:157303/1‑93 (MQ=255)

                         tGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGtatta                                                                <  2:5142554/93‑1 (MQ=255)

                          ggTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGtattat                                                               <  2:2049731/93‑1 (MQ=255)

                            tCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTg                                                             >  2:3560880/1‑93 (MQ=255)

                            tCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTg                                                             >  1:5621220/1‑93 (MQ=255)

                              gccgccCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGgc                                                           >  1:3892046/1‑93 (MQ=255)

                              gccgccCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGgc                                                           <  1:3471352/93‑1 (MQ=255)

                               ccgccCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGgcg                                                          <  1:5931026/93‑1 (MQ=255)

                                cgccCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGgcgc                                                         <  2:5463579/93‑1 (MQ=255)

                                 gccCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCa                                                        >  1:4491094/1‑93 (MQ=255)

                                 gccCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCa                                                        >  1:19704/1‑93 (MQ=255)

                                 gccCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCa                                                        >  1:2813958/1‑93 (MQ=255)

                                  cccATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCAt                                                       >  2:2261195/1‑93 (MQ=255)

                                  cccATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCAt                                                       >  2:4400779/1‑93 (MQ=255)

                                    cATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTg                                                     >  1:652944/1‑93 (MQ=255)

                                    cATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTg                                                     >  1:572285/1‑93 (MQ=255)

                                    cATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTg                                                     >  2:2952169/1‑93 (MQ=255)

                                    cATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTg                                                     >  2:2439244/1‑93 (MQ=255)

                                    cATCCTGAAGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTg                                                     >  2:3216927/1‑93 (MQ=255)

                                     aTCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGc                                                    >  2:567357/1‑93 (MQ=255)

                                      tCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCt                                                   >  1:683997/1‑93 (MQ=255)

                                      tCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCt                                                   <  2:1749329/93‑1 (MQ=255)

                                       ccTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCtt                                                  <  2:4331842/93‑1 (MQ=255)

                                        cTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTg                                                 >  1:2038005/1‑93 (MQ=255)

                                        cTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTg                                                 >  2:4602001/1‑93 (MQ=255)

                                        cTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTg                                                 >  1:4363612/1‑93 (MQ=255)

                                         tGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTgg                                                >  2:88969/1‑93 (MQ=255)

                                         tGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTgg                                                <  1:4275529/93‑1 (MQ=255)

                                         tGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTgg                                                <  2:1612520/93‑1 (MQ=255)

                                           aCGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGc                                              <  2:4898645/93‑1 (MQ=255)

                                             gCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCaa                                            >  1:1918539/1‑93 (MQ=255)

                                              cTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCaaa                                           >  2:3727266/1‑93 (MQ=255)

                                               tttAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCaaaa                                          >  2:3047388/1‑93 (MQ=255)

                                                ttAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCaaaaa                                         <  1:875215/93‑1 (MQ=255)

                                                 tAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAAc                                        <  1:5349078/93‑1 (MQ=255)

                                                    gCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGt                                     <  2:778124/93‑1 (MQ=255)

                                                     cATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTc                                    <  2:4670487/93‑1 (MQ=255)

                                                      attattGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCg                                   >  2:1805124/1‑93 (MQ=255)

                                                      attattGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCg                                   >  1:2999478/1‑93 (MQ=255)

                                                       ttattGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGCGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGc                                  >  1:6028169/1‑93 (MQ=255)

                                                         attGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCg                                >  1:1150238/1‑93 (MQ=255)

                                                         attGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCg                                >  2:2347092/1‑93 (MQ=255)

                                                          ttGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGt                               <  2:5065706/93‑1 (MQ=255)

                                                            gATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTat                             >  2:4862203/1‑93 (MQ=255)

                                                              tGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTatga                           >  2:3654216/1‑93 (MQ=255)

                                                               gCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTatgat                          <  1:2722371/93‑1 (MQ=255)

                                                                 cGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGc                        >  1:1007595/1‑93 (MQ=255)

                                                                 cGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGc                        <  1:2346799/93‑1 (MQ=255)

                                                                 cGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGc                        <  1:967526/93‑1 (MQ=255)

                                                                  ggAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCa                       <  2:4357122/93‑1 (MQ=255)

                                                                  ggAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCa                       >  2:703887/1‑93 (MQ=255)

                                                                  ggAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCa                       <  1:5003481/93‑1 (MQ=255)

                                                                   gAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAg                      >  2:3501364/1‑93 (MQ=255)

                                                                   gAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAg                      >  2:1889833/1‑93 (MQ=255)

                                                                   gAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAg                      >  1:2612817/1‑93 (MQ=255)

                                                                     aaTGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGcc                    <  1:3979452/93‑1 (MQ=255)

                                                                      aTGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCt                   <  2:3151263/93‑1 (MQ=255)

                                                                      aTGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCt                   >  2:1390973/1‑93 (MQ=255)

                                                                        gCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCtgt                 >  2:1075678/1‑93 (MQ=255)

                                                                           ggCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCa              <  1:1806741/93‑1 (MQ=255)

                                                                              aTGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGc           >  1:4728540/1‑93 (MQ=255)

                                                                              aTGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGc           >  1:5207837/1‑93 (MQ=255)

                                                                              aTGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGc           >  1:1475320/1‑93 (MQ=255)

                                                                              aTGGCGAGAGGCAGCATGGCCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGc           >  2:6113480/1‑93 (MQ=255)

                                                                               tGGCGAGCGGCAGCATTGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCt          >  1:2476965/1‑93 (MQ=255)

                                                                               tGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCt          >  1:4660726/1‑93 (MQ=255)

                                                                               tGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCt          >  2:1233951/1‑93 (MQ=255)

                                                                                 gCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCtgt        <  2:278025/93‑1 (MQ=255)

                                                                                     gcggcaGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTcg    >  2:5237444/1‑93 (MQ=255)

                                                                                      cggcagCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTcgc   <  2:4538601/93‑1 (MQ=255)

GCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCG  >  NC_000913/4392868‑4393047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACG  >  NC_000913/4392865‑4393051

GTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCA                                                                                               >  SRR4302533.2395687/1‑94 (MQ=60)

GTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCA                                                                                               >  SRR4302533.4052239/1‑94 (MQ=60)

GTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCA                                                                                               >  SRR4302533.5520275/1‑94 (MQ=60)

GTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCA                                                                                               <  SRR4302533.2103224/94‑1 (MQ=60)

 TAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGACGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGTGAGCGGCAG                                                                                              <  SRR4302533.3765384/94‑1 (MQ=60)

    CGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCAT                                                                                           <  SRR4302533.4916163/94‑1 (MQ=60)

     GGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATG                                                                                          >  SRR4302533.2883091/1‑94 (MQ=60)

      GTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTTATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGG                                                                                         <  SRR4302533.3125014/94‑1 (MQ=60)

      GTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGG                                                                                         <  SRR4302533.132800/94‑1 (MQ=60)

      GTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGG                                                                                         <  SRR4302533.4690639/94‑1 (MQ=60)

      GTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGG                                                                                         <  SRR4302533.799391/94‑1 (MQ=60)

       TGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGG                                                                                        >  SRR4302533.4244174/1‑94 (MQ=60)

       TGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGG                                                                                        <  SRR4302533.3086512/94‑1 (MQ=60)

         CTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCG                                                                                      >  SRR4302533.1465064/1‑94 (MQ=60)

         CTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCG                                                                                      <  SRR4302533.3675409/94‑1 (MQ=60)

         CTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCG                                                                                      >  SRR4302533.3339826/1‑94 (MQ=60)

           GAAAGGTGCCGCAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGAT                                                                                    <  SRR4302533.1274692/94‑1 (MQ=60)

            AAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATG                                                                                   <  SRR4302533.1532836/94‑1 (MQ=60)

             AAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGT                                                                                  <  SRR4302533.1637231/94‑1 (MQ=60)

             AAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGT                                                                                  >  SRR4302533.3250131/1‑94 (MQ=60)

              AGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTG                                                                                 <  SRR4302533.2166977/94‑1 (MQ=60)

              AGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTG                                                                                 >  SRR4302533.3101012/1‑94 (MQ=60)

               GGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGCGC                                                                                >  SRR4302533.5837376/1‑94 (MQ=60)

                 TGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTC                                                                              <  SRR4302533.2647976/94‑1 (MQ=60)

                  GCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCT                                                                             >  SRR4302533.1083326/1‑94 (MQ=60)

                  GCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCT                                                                             <  SRR4302533.1184661/94‑1 (MQ=60)

                   CCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTC                                                                            <  SRR4302533.103937/94‑1 (MQ=60)

                    CGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCT                                                                           <  SRR4302533.4373221/94‑1 (MQ=60)

                    CGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCT                                                                           >  SRR4302533.2700036/1‑94 (MQ=60)

                     GGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTG                                                                          <  SRR4302533.4033911/94‑1 (MQ=60)

                     GGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTG                                                                          >  SRR4302533.4803251/1‑94 (MQ=60)

                     GGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTG                                                                          <  SRR4302533.6249248/94‑1 (MQ=60)

                      GAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGG                                                                         <  SRR4302533.1937728/94‑1 (MQ=60)

                       AACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGT                                                                        <  SRR4302533.2813765/94‑1 (MQ=60)

                        ACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTA                                                                       <  SRR4302533.45955/94‑1 (MQ=60)

                        ACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTA                                                                       >  SRR4302533.5188217/1‑94 (MQ=60)

                         CCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTAT                                                                      <  SRR4302533.2925397/94‑1 (MQ=60)

                         CCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTAT                                                                      <  SRR4302533.21771/94‑1 (MQ=60)

                            TGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTAT                                                                   <  SRR4302533.5663884/94‑1 (MQ=60)

                            TGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTAT                                                                   <  SRR4302533.813418/94‑1 (MQ=60)

                             GGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATT                                                                  <  SRR4302533.4373628/94‑1 (MQ=60)

                              GTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTG                                                                 >  SRR4302533.5655197/1‑94 (MQ=60)

                               TCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGG                                                                <  SRR4302533.1252976/94‑1 (MQ=60)

                               TCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGG                                                                <  SRR4302533.686152/94‑1 (MQ=60)

                                CGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGC                                                               <  SRR4302533.4857205/94‑1 (MQ=60)

                                CGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGC                                                               <  SRR4302533.5990989/94‑1 (MQ=60)

                                CGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGC                                                               >  SRR4302533.3915801/1‑94 (MQ=60)

                                 GCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGTG                                                              <  SRR4302533.5979349/94‑1 (MQ=60)

                                 GCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCG                                                              <  SRR4302533.3492778/94‑1 (MQ=60)

                                  CCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGC                                                             >  SRR4302533.5513652/1‑94 (MQ=60)

                                  CCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGC                                                             <  SRR4302533.5966882/94‑1 (MQ=60)

                                   CGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCA                                                            >  SRR4302533.4518295/1‑94 (MQ=60)

                                   CGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCA                                                            <  SRR4302533.932062/94‑1 (MQ=60)

                                   CGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCA                                                            >  SRR4302533.19837/1‑94 (MQ=60)

                                   CGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCA                                                            >  SRR4302533.6304799/1‑94 (MQ=60)

                                   CGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCA                                                            <  SRR4302533.2286734/94‑1 (MQ=60)

                                   CGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCA                                                            >  SRR4302533.2831277/1‑94 (MQ=60)

                                    GCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCAT                                                           <  SRR4302533.1972647/94‑1 (MQ=60)

                                    GCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCAT                                                           <  SRR4302533.5214513/94‑1 (MQ=60)

                                     CCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATT                                                          <  SRR4302533.6252336/94‑1 (MQ=60)

                                     CCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATT                                                          >  SRR4302533.3960317/1‑94 (MQ=60)

                                      CCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTG                                                         <  SRR4302533.6103622/94‑1 (MQ=60)

                                      CCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTG                                                         <  SRR4302533.2458574/94‑1 (MQ=60)

                                      CCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTG                                                         >  SRR4302533.575662/1‑94 (MQ=60)

                                      CCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTG                                                         >  SRR4302533.656799/1‑94 (MQ=60)

                                        ATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCT                                                       >  SRR4302533.688043/1‑94 (MQ=60)

                                        ATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCT                                                       <  SRR4302533.116095/94‑1 (MQ=60)

                                        ATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCT                                                       <  SRR4302533.902612/94‑1 (MQ=60)

                                          CCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTG                                                     >  SRR4302533.4389951/1‑94 (MQ=60)

                                          CCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTG                                                     >  SRR4302533.2050574/1‑94 (MQ=60)

                                           CTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGG                                                    >  SRR4302533.3001843/1‑94 (MQ=60)

                                           CTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGG                                                    <  SRR4302533.4948332/94‑1 (MQ=60)

                                            TGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGG                                                   <  SRR4302533.4301247/94‑1 (MQ=60)

                                            TGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGG                                                   <  SRR4302533.1563484/94‑1 (MQ=60)

                                            TGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGG                                                   >  SRR4302533.4286250/1‑94 (MQ=60)

                                             GACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGC                                                  <  SRR4302533.191055/94‑1 (MQ=60)

                                              ACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCA                                                 >  SRR4302533.3620571/1‑94 (MQ=60)

                                               CGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAA                                                >  SRR4302533.1930249/1‑94 (MQ=60)

                                                 CTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAA                                              >  SRR4302533.835931/1‑94 (MQ=60)

                                                 CTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAA                                              >  SRR4302533.4549061/1‑94 (MQ=60)

                                                 CTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAA                                              >  SRR4302533.2092838/1‑94 (MQ=60)

                                                  TTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCGTTGCTTGGGCAAATA                                             >  SRR4302533.4701872/1‑94 (MQ=60)

                                                  TTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAA                                             >  SRR4302533.3855/1‑94 (MQ=60)

                                                  TTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAA                                             >  SRR4302533.2802953/1‑94 (MQ=60)

                                                  TTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAA                                             >  SRR4302533.1167449/1‑94 (MQ=60)

                                                  TTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAA                                             <  SRR4302533.5215751/94‑1 (MQ=60)

                                                  TTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTCGGCAAAAA                                             <  SRR4302533.571874/94‑1 (MQ=60)

                                                   TTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAAC                                            <  SRR4302533.6160398/94‑1 (MQ=60)

                                                   TTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAAC                                            <  SRR4302533.880351/94‑1 (MQ=60)

                                                    TAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACT                                           <  SRR4302533.5381169/94‑1 (MQ=60)

                                                    TAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACT                                           <  SRR4302533.353520/94‑1 (MQ=60)

                                                    TAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACT                                           >  SRR4302533.4607005/1‑94 (MQ=60)

                                                     AGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTT                                          >  SRR4302533.1887390/1‑94 (MQ=60)

                                                     AGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTG                                          <  SRR4302533.4448312/94‑1 (MQ=60)

                                                       GCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTC                                        <  SRR4302533.2029445/94‑1 (MQ=60)

                                                        CATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCG                                       >  SRR4302533.3017822/1‑94 (MQ=60)

                                                         ATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGC                                      <  SRR4302533.261505/94‑1 (MQ=60)

                                                         ATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGC                                      >  SRR4302533.3808837/1‑94 (MQ=60)

                                                         ATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGCGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGC                                      >  SRR4302533.6064703/1‑94 (MQ=60)

                                                          TTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTACTTGGGCAAAAACTGTCGCC                                     <  SRR4302533.4376330/94‑1 (MQ=60)

                                                           TATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCG                                    >  SRR4302533.1157107/1‑94 (MQ=60)

                                                              TGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTAT                                 >  SRR4302533.4685354/1‑94 (MQ=60)

                                                                ATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGA                               >  SRR4302533.4010233/1‑94 (MQ=60)

                                                                 TGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGAT                              <  SRR4302533.5801983/94‑1 (MQ=60)

                                                                  GCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATG                             <  SRR4302533.2739078/94‑1 (MQ=60)

                                                                   CCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGC                            <  SRR4302533.3466165/94‑1 (MQ=60)

                                                                   CCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGC                            >  SRR4302533.1013489/1‑94 (MQ=60)

                                                                   CCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGC                            <  SRR4302533.4331408/94‑1 (MQ=60)

                                                                   CCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGC                            >  SRR4302533.4422487/1‑94 (MQ=60)

                                                                    CGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCA                           <  SRR4302533.973200/94‑1 (MQ=60)

                                                                    CGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCA                           <  SRR4302533.2361322/94‑1 (MQ=60)

                                                                     GGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAG                          <  SRR4302533.5033806/94‑1 (MQ=60)

                                                                     GGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAG                          >  SRR4302533.2628854/1‑94 (MQ=60)

                                                                     GGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGCATGATGCAG                          <  SRR4302533.2081681/94‑1 (MQ=60)

                                                                        AATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCT                       <  SRR4302533.5034574/94‑1 (MQ=60)

                                                                        AATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCT                       <  SRR4302533.4003677/94‑1 (MQ=60)

                                                                         ATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTG                      >  SRR4302533.2584515/1‑94 (MQ=60)

                                                                           GCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTG                    >  SRR4302533.4989690/1‑94 (MQ=60)

                                                                            CAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGC                   >  SRR4302533.4349517/1‑94 (MQ=60)

                                                                            CAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGC                   <  SRR4302533.1639148/94‑1 (MQ=60)

                                                                              GGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAG                 <  SRR4302533.1817737/94‑1 (MQ=60)

                                                                              GGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAG                 <  SRR4302533.2026616/94‑1 (MQ=60)

                                                                              GGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAG                 >  SRR4302533.1428251/1‑94 (MQ=60)

                                                                              GGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAG                 <  SRR4302533.3095231/94‑1 (MQ=60)

                                                                              GGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAG                 <  SRR4302533.3006437/94‑1 (MQ=60)

                                                                                CATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGC               >  SRR4302533.1484237/1‑94 (MQ=60)

                                                                                CATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGC               >  SRR4302533.4757208/1‑94 (MQ=60)

                                                                                CATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGC               >  SRR4302533.511666/1‑94 (MQ=60)

                                                                                CATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGC               >  SRR4302533.5239211/1‑94 (MQ=60)

                                                                                 ATGGCGAGCGGCAGCATTGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCT              >  SRR4302533.2492241/1‑94 (MQ=60)

                                                                                 ATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCT              >  SRR4302533.4545318/1‑94 (MQ=60)

                                                                                 ATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCT              >  SRR4302533.5266365/1‑94 (MQ=60)

                                                                                 ATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCT              <  SRR4302533.3247860/94‑1 (MQ=60)

                                                                                 ATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCT              >  SRR4302533.4688970/1‑94 (MQ=60)

                                                                                     CGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGT          <  SRR4302533.5468889/94‑1 (MQ=60)

                                                                                      GAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTC         <  SRR4302533.714708/94‑1 (MQ=60)

                                                                                      GAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTC         <  SRR4302533.2870786/94‑1 (MQ=60)

                                                                                      GAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTC         <  SRR4302533.536822/94‑1 (MQ=60)

                                                                                       AGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCG        <  SRR4302533.2476541/94‑1 (MQ=60)

                                                                                       AGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCG        <  SRR4302533.3995575/94‑1 (MQ=60)

                                                                                       AGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCG        >  SRR4302533.720349/1‑94 (MQ=60)

                                                                                       AGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCG        <  SRR4302533.4254900/94‑1 (MQ=60)

                                                                                         CGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCG      >  SRR4302533.672259/1‑94 (MQ=60)

                                                                                          GGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGC     <  SRR4302533.1647591/94‑1 (MQ=60)

                                                                                           GCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCA    <  SRR4302533.5615842/94‑1 (MQ=60)

                                                                                            CAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCAC   <  SRR4302533.5357114/94‑1 (MQ=60)

                                                                                             AGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACG  >  SRR4302533.2073703/1‑94 (MQ=60)

                                                                                             AGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACG  >  SRR4302533.5392645/1‑94 (MQ=60)

                                                                                             AGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACG  >  SRR4302533.5300563/1‑94 (MQ=60)

GTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACG  >  NC_000913/4392865‑4393051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`