Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,427,095 A→G T195A (ACC→GCC)  b4207 → FKBP‑type 22KD peptidyl‑prolyl cis‑trans isomerase (rotamase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,427,0950AG99.3% 1338.2 / ‑2.9 427T195A (ACC→GCC) b4207FKBP‑type 22KD peptidyl‑prolyl cis‑trans isomerase (rotamase)
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (253/171);  total (255/172)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 446 total aligned reads displayed.

ATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCACCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATA  >  NC_000913/4427005‑4427184
                                                                                          |                                                                                         
aTGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGcc                                                                                         <  1:8333197/93‑1 (MQ=255)
aTGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGcc                                                                                         >  2:10026071/1‑93 (MQ=255)
aTGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGcc                                                                                         >  2:3354343/1‑93 (MQ=255)
 tGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGccc                                                                                        >  1:2190741/1‑93 (MQ=255)
   gcGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTg                                                                                      <  2:8648247/92‑1 (MQ=255)
   ccGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTg                                                                                      <  1:8859009/93‑1 (MQ=255)
   ccGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCCCCGTTCAGCGCCCTg                                                                                      >  1:2561247/1‑93 (MQ=255)
    cGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTgg                                                                                     <  1:4072730/93‑1 (MQ=255)
     ggTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGgt                                                                                    >  1:6385147/1‑93 (MQ=255)
     ggTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGgt                                                                                    >  1:9650565/1‑93 (MQ=255)
     ggTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGgt                                                                                    >  2:7253115/1‑93 (MQ=255)
     ggTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCACCCCTCCGTTCAGCGCCCTGgt                                                                                    <  2:5660137/93‑1 (MQ=255)
      gTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGgtg                                                                                   >  2:9685952/1‑93 (MQ=255)
       tAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGgtgt                                                                                  >  2:107030/1‑93 (MQ=255)
       tAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGAGCCCTGgtgt                                                                                  >  2:6069755/1‑93 (MQ=255)
        aGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGtt                                                                                 <  2:7914923/93‑1 (MQ=255)
         ggTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGttt                                                                                <  2:35298/93‑1 (MQ=255)
         ggTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGttt                                                                                >  1:2970745/1‑93 (MQ=255)
         ggTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGttt                                                                                <  2:7432117/93‑1 (MQ=255)
         ggTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGttt                                                                                >  2:7730734/1‑93 (MQ=255)
          gTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTg                                                                               >  2:2748127/1‑93 (MQ=255)
           ttCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGa                                                                              >  2:7559066/1‑93 (MQ=255)
            tCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGaa                                                                             <  1:9932807/93‑1 (MQ=255)
            tCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGaa                                                                             <  1:5271961/93‑1 (MQ=255)
             cTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAg                                                                            >  2:3469720/1‑93 (MQ=255)
             cTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAg                                                                            >  1:8698227/1‑93 (MQ=255)
             cTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAg                                                                            >  2:9371841/1‑93 (MQ=255)
              tAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGt                                                                           <  1:111022/93‑1 (MQ=255)
              tAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGt                                                                           <  2:8426578/93‑1 (MQ=255)
               aaaTGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTc                                                                          <  2:9563183/93‑1 (MQ=255)
                 aTGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGa                                                                        >  1:9117327/1‑93 (MQ=255)
                 aTGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGa                                                                        >  2:4159982/1‑93 (MQ=255)
                 aTGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGa                                                                        <  1:3255246/93‑1 (MQ=255)
                  tGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGaa                                                                       <  1:6517334/93‑1 (MQ=255)
                  tGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGaa                                                                       <  1:7885299/93‑1 (MQ=255)
                  agggAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGaa                                                                       <  1:7168788/92‑1 (MQ=255)
                      aaCTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAActgc                                                                   <  2:7069517/93‑1 (MQ=255)
                        cTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAActgctg                                                                 <  1:3426758/93‑1 (MQ=255)
                         tGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTgg                                                                >  1:3631701/1‑93 (MQ=255)
                         tGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCACTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTgg                                                                >  1:4924372/1‑93 (MQ=255)
                         cgACTATCCCGCAGGAACTGGCATATGGCGAGCGCGCCGCAGGCGCATCCATCCCTCCGTGCAGCGCCCTGATGTTTGAAGTCGAACtggtgg                                                                <  2:1276573/92‑1 (MQ=255)
                             tATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAAt                                                            >  1:9364599/1‑93 (MQ=255)
                             tATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAAt                                                            >  1:2376631/1‑93 (MQ=255)
                             tATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAAt                                                            >  1:7579537/1‑93 (MQ=255)
                               tCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATcc                                                          >  2:9830329/1‑93 (MQ=255)
                               tCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATcc                                                          >  1:3164121/1‑93 (MQ=255)
                               tCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATcc                                                          >  1:9455755/1‑93 (MQ=255)
                                cccGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCct                                                         >  1:3734935/1‑93 (MQ=255)
                                 ccGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCctc                                                        >  1:1911150/1‑93 (MQ=255)
                                 ccGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCctc                                                        <  1:5069041/93‑1 (MQ=255)
                                  cGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCctct                                                       >  2:1334986/1‑93 (MQ=255)
                                   gCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTa                                                      <  2:3308313/93‑1 (MQ=255)
                                     aGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAag                                                    >  2:1450623/1‑93 (MQ=255)
                                      ggAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAagc                                                   <  1:1377163/93‑1 (MQ=255)
                                      ggAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCCCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAagc                                                   <  2:6514254/93‑1 (MQ=255)
                                       gAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAagca                                                  <  2:5265243/93‑1 (MQ=255)
                                       gAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAagca                                                  >  2:3674314/1‑93 (MQ=255)
                                       aaaatGGCATAAAGAGAGCGAGGCGCAGGAGCATCCATACCTCCATTCAACGCAATGGTATTTGAAGTCGAACTGCTGGAAATACTATAaaca                                                  <  1:8943485/89‑1 (MQ=255)
                                        aaCTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAagcag                                                 >  2:256017/1‑93 (MQ=255)
                                          cTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAgcg                                               >  1:9020886/1‑93 (MQ=255)
                                           tGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCATCTAAGCAgcgc                                              >  1:768210/1‑93 (MQ=255)
                                              cATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCAtt                                           >  1:5969879/1‑93 (MQ=255)
                                                tatGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCt                                         >  2:2507385/1‑93 (MQ=255)
                                                tatGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCt                                         >  2:313774/1‑93 (MQ=255)
                                                  tGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGt                                       <  2:9234719/93‑1 (MQ=255)
                                                   ggCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGtt                                      <  2:391939/93‑1 (MQ=255)
                                                     cGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGACGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTcc                                    >  1:6657430/1‑93 (MQ=255)
                                                      gAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTccc                                   >  2:6172321/1‑93 (MQ=255)
                                                          gcggcgCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCg                               >  1:5538017/1‑93 (MQ=255)
                                                          gcggcgCAGGCGCACCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTca                               <  2:4957372/93‑2 (MQ=255)
                                                            ggcgcaggcgcaTCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCACTCGaa                             >  2:9160731/1‑93 (MQ=255)
                                                            cgcgcaggcccaTCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGaa                             <  2:1985133/92‑1 (MQ=255)
                                                             gcgcaggggcaTCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAAc                            <  1:4688542/93‑1 (MQ=255)
                                                             gcgcaggcgcaTCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAAc                            >  1:4785049/1‑93 (MQ=255)
                                                               gcaggcgcaTCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACga                          <  1:8479544/93‑1 (MQ=255)
                                                                caggcgcaTCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACgag                         <  2:9873857/93‑1 (MQ=255)
                                                                 aggcgcaTCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACgaga                        >  2:1673661/1‑93 (MQ=255)
                                                                 aggcgcaTCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACgaga                        <  1:3925825/93‑1 (MQ=255)
                                                                 aggcgcaTCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTAGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACgaga                        <  2:4292589/93‑1 (MQ=255)
                                                                   gcgcaTCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAgg                      <  1:8147029/93‑1 (MQ=255)
                                                                    cgcaTCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAggg                     <  2:7936574/93‑1 (MQ=255)
                                                                     gcaTCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAgggg                    <  2:5878811/93‑1 (MQ=255)
                                                                     gcaTCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAgggg                    <  1:2686109/93‑1 (MQ=255)
                                                                       ctccatccCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAg                  <  1:6946421/92‑1 (MQ=255)
                                                                        tccatccCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGc                 <  2:4533804/93‑1 (MQ=255)
                                                                          catccCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACCGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAg               >  2:4727548/1‑93 (MQ=255)
                                                                            tccCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGc             >  1:6280545/1‑93 (MQ=255)
                                                                            tccCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGc             <  1:1566165/93‑1 (MQ=255)
                                                                             cccTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCa            <  2:675214/93‑1 (MQ=255)
                                                                             cccTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCa            <  2:1868691/93‑1 (MQ=255)
                                                                                tCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTc         >  2:227081/1‑93 (MQ=255)
                                                                                 ccGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCa        >  2:4054338/1‑93 (MQ=255)
                                                                                 ccGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCa        >  1:10035972/1‑93 (MQ=255)
                                                                                  cGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAg       <  1:4350018/93‑1 (MQ=255)
                                                                                   gTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGc      >  1:1709982/1‑93 (MQ=255)
                                                                                     tCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCaa    >  1:6731803/1‑93 (MQ=255)
                                                                                     tCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCaa    >  1:5436981/1‑93 (MQ=255)
                                                                                      cAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAAt   >  2:319438/1‑93 (MQ=255)
                                                                                      cAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAAt   <  2:8885746/93‑1 (MQ=255)
                                                                                       aGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGTAGGCATTCAGCAATa  <  1:3037316/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCACCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATA  >  NC_000913/4427005‑4427184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 231 total aligned reads displayed.
CTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCACCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAACC  >  NC_000913/4427002‑4427188
                                                                                             |                                                                                             
CTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCG                                                                                               <  SRR4302540.3422574/94‑1 (MQ=60)
  GATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCC                                                                                             >  SRR4302540.7694393/1‑94 (MQ=60)
  GATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCC                                                                                             >  SRR4302540.8362122/1‑94 (MQ=60)
  GATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCC                                                                                             >  SRR4302540.5015718/1‑94 (MQ=60)
  GATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCC                                                                                             >  SRR4302540.6651881/1‑94 (MQ=60)
   ATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCC                                                                                            >  SRR4302540.9903584/1‑94 (MQ=60)
   ATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCC                                                                                            <  SRR4302540.8429928/94‑1 (MQ=60)
   ATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCC                                                                                            >  SRR4302540.2214396/1‑94 (MQ=60)
   ATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCACGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCC                                                                                            <  SRR4302540.8717039/94‑1 (MQ=60)
    TGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCT                                                                                           >  SRR4302540.9191306/1‑94 (MQ=60)
     GCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTG                                                                                          >  SRR4302540.9616543/1‑94 (MQ=60)
     GCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTG                                                                                          >  SRR4302540.214959/1‑94 (MQ=60)
     GCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCCCCGTTCAGCGCCCTG                                                                                          >  SRR4302540.2588858/1‑94 (MQ=60)
      CCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGG                                                                                         >  SRR4302540.10062915/1‑94 (MQ=60)
       CGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGT                                                                                        <  SRR4302540.4116631/94‑1 (MQ=60)
       CGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGT                                                                                        >  SRR4302540.9764200/1‑94 (MQ=60)
       CGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGT                                                                                        >  SRR4302540.10081512/1‑94 (MQ=60)
       CGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGT                                                                                        >  SRR4302540.6456227/1‑94 (MQ=60)
       CGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGG                                                                                        >  SRR4302540.4919814/1‑94 (MQ=60)
         GTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGT                                                                                      >  SRR4302540.2328932/1‑94 (MQ=60)
         GTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGT                                                                                      >  SRR4302540.5773608/1‑94 (MQ=60)
          TAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTT                                                                                     >  SRR4302540.67842/1‑94 (MQ=60)
          TAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTT                                                                                     >  SRR4302540.9484897/1‑94 (MQ=60)
           AGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTT                                                                                    >  SRR4302540.6148317/1‑94 (MQ=60)
           AGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTT                                                                                    <  SRR4302540.6409932/94‑1 (MQ=60)
           AGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTT                                                                                    >  SRR4302540.3003557/1‑94 (MQ=60)
           AGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTT                                                                                    >  SRR4302540.4658035/1‑94 (MQ=60)
            GGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTG                                                                                   >  SRR4302540.3958440/1‑94 (MQ=60)
            GGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTG                                                                                   >  SRR4302540.5009986/1‑94 (MQ=60)
              TTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAA                                                                                 >  SRR4302540.3678061/1‑94 (MQ=60)
              TTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAA                                                                                 <  SRR4302540.8107255/94‑1 (MQ=60)
              TTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAA                                                                                 >  SRR4302540.4964845/1‑94 (MQ=60)
               TCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAG                                                                                <  SRR4302540.5329509/94‑1 (MQ=60)
               TCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAG                                                                                <  SRR4302540.10048730/94‑1 (MQ=60)
               TCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAG                                                                                <  SRR4302540.2862996/94‑1 (MQ=60)
               TCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAG                                                                                >  SRR4302540.9054050/1‑94 (MQ=60)
               TCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAG                                                                                >  SRR4302540.8799198/1‑94 (MQ=60)
                CTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGT                                                                               <  SRR4302540.5576219/94‑1 (MQ=60)
                CTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGT                                                                               >  SRR4302540.5205771/1‑94 (MQ=60)
                CTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGT                                                                               <  SRR4302540.9700377/94‑1 (MQ=60)
                CTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAACGCGGCGACGGGTCATCCATGCCTCCGTTCAGCGCCCTGGTGTCTGAAGT                                                                               >  SRR4302540.5205772/1‑94 (MQ=60)
                 TAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTC                                                                              <  SRR4302540.112209/94‑1 (MQ=60)
                  AAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCG                                                                             >  SRR4302540.4750854/1‑94 (MQ=60)
                  AAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCG                                                                             >  SRR4302540.4431154/1‑94 (MQ=60)
                   AATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGA                                                                            >  SRR4302540.9212848/1‑94 (MQ=60)
                   AATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGA                                                                            >  SRR4302540.9226917/1‑94 (MQ=60)
                   AATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGA                                                                            >  SRR4302540.7409157/1‑94 (MQ=60)
                    ATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAA                                                                           >  SRR4302540.4717047/1‑94 (MQ=60)
                    ATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAA                                                                           <  SRR4302540.5356272/94‑1 (MQ=60)
                    ATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAA                                                                           >  SRR4302540.3393002/1‑94 (MQ=60)
                    ATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAA                                                                           <  SRR4302540.8947503/94‑1 (MQ=60)
                    ATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAA                                                                           <  SRR4302540.3290729/94‑1 (MQ=60)
                     TGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAAC                                                                          <  SRR4302540.1077724/94‑1 (MQ=60)
                     TGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAAC                                                                          <  SRR4302540.6589728/94‑1 (MQ=60)
                     TGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAAC                                                                          >  SRR4302540.6569741/1‑94 (MQ=60)
                     TGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAAC                                                                          <  SRR4302540.7977359/94‑1 (MQ=60)
                     AGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAAC                                                                          <  SRR4302540.7248705/94‑1 (MQ=60)
                      GGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACT                                                                         >  SRR4302540.4917757/1‑94 (MQ=60)
                       GGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTG                                                                        <  SRR4302540.4067396/94‑1 (MQ=60)
                       GGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTG                                                                        <  SRR4302540.2431249/94‑1 (MQ=60)
                        GAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGC                                                                       >  SRR4302540.8174069/1‑94 (MQ=60)
                         AACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCT                                                                      <  SRR4302540.8924500/94‑1 (MQ=60)
                         AACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCT                                                                      <  SRR4302540.6736153/94‑1 (MQ=60)
                          ACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTG                                                                     >  SRR4302540.5820623/1‑94 (MQ=60)
                          ACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTG                                                                     >  SRR4302540.95021/1‑94 (MQ=60)
                          ACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTG                                                                     >  SRR4302540.6572748/1‑94 (MQ=60)
                           CTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGG                                                                    >  SRR4302540.4814418/1‑94 (MQ=60)
                           CTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGG                                                                    <  SRR4302540.3464001/94‑1 (MQ=60)
                           CTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGG                                                                    >  SRR4302540.3671451/1‑94 (MQ=60)
                            TGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGA                                                                   >  SRR4302540.936237/1‑94 (MQ=60)
                            TGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGA                                                                   >  SRR4302540.4989453/1‑94 (MQ=60)
                             GACTATCCCGCAGGCACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAA                                                                  >  SRR4302540.5943819/1‑94 (MQ=60)
                             GACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAA                                                                  >  SRR4302540.7678067/1‑94 (MQ=60)
                             GACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAA                                                                  <  SRR4302540.624048/94‑1 (MQ=60)
                             GACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAA                                                                  <  SRR4302540.7000698/94‑1 (MQ=60)
                              ACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAA                                                                 >  SRR4302540.9002711/1‑94 (MQ=60)
                              ACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGAGTTTGAAGTGGAACTGCTGGAAA                                                                 >  SRR4302540.9002713/1‑94 (MQ=60)
                              ACTAGCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAA                                                                 <  SRR4302540.3792449/94‑1 (MQ=60)
                               CTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAAT                                                                >  SRR4302540.2402245/1‑94 (MQ=60)
                               CTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAAT                                                                >  SRR4302540.7664597/1‑94 (MQ=60)
                               CTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAAT                                                                >  SRR4302540.9476109/1‑94 (MQ=60)
                               CTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAAT                                                                <  SRR4302540.4466950/94‑1 (MQ=60)
                               CTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAAT                                                                >  SRR4302540.819195/1‑94 (MQ=60)
                                 ATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCC                                                              <  SRR4302540.3211275/94‑1 (MQ=60)
                                 ATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCC                                                              >  SRR4302540.9567971/1‑94 (MQ=60)
                                 ATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCC                                                              >  SRR4302540.3198535/1‑94 (MQ=60)
                                  TCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCT                                                             >  SRR4302540.3775686/1‑94 (MQ=60)
                                  TCCCGCAGGAACTGGCATATGGCGAGCCCGCCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCT                                                             <  SRR4302540.6988068/94‑1 (MQ=60)
                                   CCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTC                                                            >  SRR4302540.1932030/1‑94 (MQ=60)
                                   CCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTC                                                            >  SRR4302540.5374935/1‑94 (MQ=60)
                                    CCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCT                                                           <  SRR4302540.5123848/94‑1 (MQ=60)
                                    CCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCT                                                           >  SRR4302540.1806705/1‑94 (MQ=60)
                                    CCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGGTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCT                                                           >  SRR4302540.2650536/1‑94 (MQ=60)
                                     CGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTA                                                          >  SRR4302540.3185122/1‑94 (MQ=60)
                                      GCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAA                                                         <  SRR4302540.5495564/94‑1 (MQ=60)
                                      GCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAA                                                         >  SRR4302540.3418425/1‑94 (MQ=60)
                                      GCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAA                                                         >  SRR4302540.1534778/1‑94 (MQ=60)
                                       CAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAG                                                        >  SRR4302540.1496764/1‑94 (MQ=60)
                                       CAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAG                                                        <  SRR4302540.4901310/94‑1 (MQ=60)
                                       CAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGCGTTTGAAGTCGAACTGCTGGAAATCCTCTAAG                                                        >  SRR4302540.6402664/1‑94 (MQ=60)
                                        AGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGC                                                       >  SRR4302540.5525697/1‑94 (MQ=60)
                                        AGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGC                                                       >  SRR4302540.9266572/1‑94 (MQ=60)
                                        AGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGC                                                       >  SRR4302540.8839253/1‑94 (MQ=60)
                                         GGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCA                                                      <  SRR4302540.1391809/94‑1 (MQ=60)
                                         GGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCA                                                      <  SRR4302540.317442/94‑1 (MQ=60)
                                         GGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGAAAATCCTCTAAGCA                                                      <  SRR4302540.9538444/94‑1 (MQ=60)
                                          GAACTGGCATATGGCGAGCGCCGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAG                                                     <  SRR4302540.6857778/94‑1 (MQ=60)
                                           AACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGC                                                    >  SRR4302540.3835948/1‑94 (MQ=60)
                                            ACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCG                                                   >  SRR4302540.9129130/1‑94 (MQ=60)
                                             CTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGC                                                  >  SRR4302540.9904336/1‑94 (MQ=60)
                                             CTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCATCTAAGCAGCGC                                                  >  SRR4302540.776395/1‑94 (MQ=60)
                                                 CATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTC                                              <  SRR4302540.3717760/94‑1 (MQ=60)
                                                 CATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTC                                              >  SRR4302540.3063199/1‑94 (MQ=60)
                                                 CATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTC                                              >  SRR4302540.3269473/1‑94 (MQ=60)
                                                  ATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCT                                             >  SRR4302540.5652616/1‑94 (MQ=60)
                                                  ATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCT                                             >  SRR4302540.1770078/1‑94 (MQ=60)
                                                  ATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCT                                             >  SRR4302540.6366005/1‑94 (MQ=60)
                                                  ATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCT                                             >  SRR4302540.7630757/1‑94 (MQ=60)
                                                   TATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTT                                            >  SRR4302540.2713823/1‑94 (MQ=60)
                                                   TATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTG                                            >  SRR4302540.3626667/1‑94 (MQ=60)
                                                    ATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGT                                           >  SRR4302540.3069489/1‑94 (MQ=60)
                                                       GCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGACGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCC                                        >  SRR4302540.6731042/1‑94 (MQ=60)
                                                       GCGAGCGCGGCGCAGGCGCAGCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCC                                        <  SRR4302540.10113931/94‑1 (MQ=60)
                                                        GGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCC                                       <  SRR4302540.2545850/94‑1 (MQ=60)
                                                         GAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCC                                      >  SRR4302540.6555138/1‑94 (MQ=60)
                                                         GAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCC                                      >  SRR4302540.4076088/1‑94 (MQ=60)
                                                         GAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCC                                      >  SRR4302540.2083260/1‑94 (MQ=60)
                                                          AGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCT                                     <  SRR4302540.410718/94‑1 (MQ=60)
                                                          AGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCT                                     <  SRR4302540.1495886/94‑1 (MQ=60)
                                                           GCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTC                                    <  SRR4302540.6923653/94‑1 (MQ=60)
                                                           GCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTC                                    <  SRR4302540.8529891/94‑1 (MQ=60)
                                                            CGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCG                                   >  SRR4302540.5599011/1‑94 (MQ=60)
                                                             GCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGA                                  >  SRR4302540.9640270/1‑94 (MQ=60)
                                                             GCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGA                                  <  SRR4302540.420640/94‑1 (MQ=60)
                                                              CGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAA                                 <  SRR4302540.10106860/94‑1 (MQ=60)
                                                              CGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAA                                 <  SRR4302540.8432665/94‑1 (MQ=60)
                                                               GGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAAC                                <  SRR4302540.1016755/94‑1 (MQ=60)
                                                               GGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAAC                                >  SRR4302540.4836206/1‑94 (MQ=60)
                                                                GCGCAGGGGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACG                               <  SRR4302540.4738684/94‑1 (MQ=60)
                                                                 CGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGATCTGCTGGAAATCCTCTTAGCTGCGCATTCTGTTCCCCTCTAACGA                              >  SRR4302540.9071138/1‑94 (MQ=60)
                                                                  GCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAG                             >  SRR4302540.7186092/1‑94 (MQ=60)
                                                                  GCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAG                             <  SRR4302540.8578034/94‑1 (MQ=60)
                                                                    AGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAG                           <  SRR4302540.8559560/94‑1 (MQ=60)
                                                                    AGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAG                           <  SRR4302540.8305008/94‑1 (MQ=60)
                                                                    AGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAG                           <  SRR4302540.2276342/94‑1 (MQ=60)
                                                                    AGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAG                           >  SRR4302540.3599299/1‑94 (MQ=60)
                                                                    AGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAG                           <  SRR4302540.3968299/94‑1 (MQ=60)
                                                                    AGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAG                           <  SRR4302540.8440231/94‑1 (MQ=60)
                                                                     GGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGG                          <  SRR4302540.1700560/94‑1 (MQ=60)
                                                                     GGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGG                          <  SRR4302540.1520826/94‑1 (MQ=60)
                                                                     GGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGG                          <  SRR4302540.7901045/94‑1 (MQ=60)
                                                                     GGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGG                          <  SRR4302540.7901046/94‑1 (MQ=60)
                                                                     GGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGG                          <  SRR4302540.8705501/94‑1 (MQ=60)
                                                                      GCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGG                         <  SRR4302540.1302995/94‑1 (MQ=60)
                                                                      GCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGG                         <  SRR4302540.8241606/94‑1 (MQ=60)
                                                                       CGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGG                        <  SRR4302540.8921567/94‑1 (MQ=60)
                                                                       CGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGG                        <  SRR4302540.6678004/94‑1 (MQ=60)
                                                                        GCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGA                       <  SRR4302540.2715146/94‑1 (MQ=60)
                                                                          CTCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGC                     <  SRR4302540.7023731/94‑1 (MQ=60)
                                                                            CCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAG                   <  SRR4302540.6282512/94‑1 (MQ=60)
                                                                             CATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGG                  >  SRR4302540.338666/1‑94 (MQ=60)
                                                                              ATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGC                 <  SRR4302540.6754436/94‑1 (MQ=60)
                                                                              ATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGC                 >  SRR4302540.6350434/1‑94 (MQ=60)
                                                                               TCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCA                <  SRR4302540.1583348/94‑1 (MQ=60)
                                                                                CCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCAT               >  SRR4302540.6676684/1‑94 (MQ=60)
                                                                                 CCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATT              >  SRR4302540.2800497/1‑94 (MQ=60)
                                                                                 CCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATT              >  SRR4302540.2242006/1‑94 (MQ=60)
                                                                                 CCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATT              >  SRR4302540.782008/1‑94 (MQ=60)
                                                                                  CTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTC             >  SRR4302540.3018851/1‑94 (MQ=60)
                                                                                  CTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTC             <  SRR4302540.7632911/94‑1 (MQ=60)
                                                                                   TCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCA            <  SRR4302540.4363476/94‑1 (MQ=60)
                                                                                   TCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCA            >  SRR4302540.10152690/1‑94 (MQ=60)
                                                                                   TCCGTTCAGCGCCCGGGTGTTTGAAGTCGAACTGCTATAAAGCCTCTAAGCAGCGCATGCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCA            <  SRR4302540.1720436/94‑1 (MQ=60)
                                                                                    CCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAG           <  SRR4302540.8992754/94‑1 (MQ=60)
                                                                                     CGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCATCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGC          >  SRR4302540.7602125/1‑94 (MQ=60)
                                                                                     CGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGTAGGCATTCAGC          <  SRR4302540.2147198/94‑1 (MQ=60)
                                                                                     CGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGC          >  SRR4302540.1728455/1‑94 (MQ=60)
                                                                                     CGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGC          >  SRR4302540.909118/1‑94 (MQ=60)
                                                                                     CGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGC          <  SRR4302540.3694930/94‑1 (MQ=60)
                                                                                     CGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGC          >  SRR4302540.9137303/1‑94 (MQ=60)
                                                                                     CGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGC          >  SRR4302540.1728465/1‑94 (MQ=60)
                                                                                     CGTTCAGCACCCTGGTGTTTGAAGTCGAACGGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGC          <  SRR4302540.4192311/94‑1 (MQ=60)
                                                                                       TTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAA        >  SRR4302540.5496782/1‑94 (MQ=60)
                                                                                        TCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAAT       >  SRR4302540.7123540/1‑94 (MQ=60)
                                                                                        TCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAAT       <  SRR4302540.5179991/94‑1 (MQ=60)
                                                                                         CAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATA      <  SRR4302540.2663660/94‑1 (MQ=60)
                                                                                         CAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATA      <  SRR4302540.3229120/94‑1 (MQ=60)
                                                                                         CAGCACCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATA      >  SRR4302540.9318065/1‑94 (MQ=60)
                                                                                          AGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGTAGGCATTCAGCAATAA     <  SRR4302540.3070654/94‑1 (MQ=60)
                                                                                          AGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAA     >  SRR4302540.3555527/1‑94 (MQ=60)
                                                                                          AGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAA     <  SRR4302540.3022283/94‑1 (MQ=60)
                                                                                          AGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAA     <  SRR4302540.1386619/94‑1 (MQ=60)
                                                                                            CGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAAC   <  SRR4302540.204817/94‑1 (MQ=60)
                                                                                            CGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTCCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAAC   <  SRR4302540.10145718/94‑1 (MQ=60)
                                                                                            CGCCCTGGTGTTTGAAGACGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAAC   <  SRR4302540.1984621/94‑1 (MQ=60)
                                                                                             GCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAACC  <  SRR4302540.1113631/94‑1 (MQ=60)
                                                                                             GCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAACC  >  SRR4302540.5954185/1‑94 (MQ=60)
                                                                                             GCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAACC  >  SRR4302540.4312386/1‑94 (MQ=60)
                                                                                             GCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAACC  <  SRR4302540.4656688/94‑1 (MQ=60)
                                                                                             GCCCTGGTGTTTGAAGCCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAACC  >  SRR4302540.1189491/1‑94 (MQ=60)
                                                                                             |                                                                                             
CTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCACCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAACC  >  NC_000913/4427002‑4427188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑