Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,427,095 A→G T195A (ACC→GCC)  b4207 → FKBP‑type 22KD peptidyl‑prolyl cis‑trans isomerase (rotamase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,427,0950AG100.0% 732.9 / NA 230T195A (ACC→GCC) b4207FKBP‑type 22KD peptidyl‑prolyl cis‑trans isomerase (rotamase)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (114/116);  total (114/116)

Only 100 of 233 total aligned reads displayed.

ATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCACCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAAT  >  NC_000913/4427005‑4427183
                                                                                          |                                                                                        
aTGCGGGTAGGTTCTAAATGGGAACTGACTACCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGcc                                                                                        <  2:4857331/93‑1 (MQ=255)
 tGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGccc                                                                                       <  2:3257971/93‑1 (MQ=255)
  gCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCt                                                                                      >  2:1711975/1‑93 (MQ=255)
    cGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTgg                                                                                    <  1:5084979/93‑1 (MQ=255)
     ggTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGgt                                                                                   >  1:4197443/1‑93 (MQ=255)
      gTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGgtg                                                                                  <  1:4485005/93‑1 (MQ=255)
      gTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGgtg                                                                                  <  1:1150040/93‑1 (MQ=255)
       tAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGgtgt                                                                                 <  1:2480241/93‑1 (MQ=255)
       tAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGgggt                                                                                 >  2:2446986/1‑93 (MQ=255)
        aGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGtt                                                                                >  1:2891835/1‑93 (MQ=255)
        aGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGtt                                                                                <  1:3738954/93‑1 (MQ=255)
        aGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGtt                                                                                >  2:1557535/1‑93 (MQ=255)
        aGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGtt                                                                                <  2:1647664/93‑1 (MQ=255)
         ggTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGttt                                                                               >  1:458594/1‑93 (MQ=255)
          gTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTg                                                                              <  1:861669/93‑1 (MQ=255)
          gTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTg                                                                              >  2:3034094/1‑93 (MQ=255)
          gTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTg                                                                              >  1:611348/1‑93 (MQ=255)
           ttCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGa                                                                             <  2:5565282/93‑1 (MQ=255)
            tCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGTGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGaa                                                                            <  1:3380481/93‑1 (MQ=255)
            tCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGaa                                                                            <  1:2853135/93‑1 (MQ=255)
            tCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGaa                                                                            >  1:3169527/1‑93 (MQ=255)
            tCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGaa                                                                            <  2:5439678/93‑1 (MQ=255)
              tAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGt                                                                          <  2:1956295/93‑1 (MQ=255)
              tAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGt                                                                          <  2:3482353/93‑1 (MQ=255)
              tAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGt                                                                          <  1:1133544/93‑1 (MQ=255)
                  tGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGaa                                                                      <  2:6137335/93‑1 (MQ=255)
                  tGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGAGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGCGTTTGCAGTCGaa                                                                      >  2:4242948/1‑93 (MQ=255)
                    ggAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAAct                                                                    >  1:1969541/1‑93 (MQ=255)
                     gAACTGACTATCGCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAActg                                                                   >  2:5043474/1‑93 (MQ=255)
                     gAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAActg                                                                   >  2:3217861/1‑93 (MQ=255)
                     gAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAActg                                                                   <  1:5776065/93‑1 (MQ=255)
                      aaCTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAActgc                                                                  <  1:2229088/93‑1 (MQ=255)
                       aCTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAActgct                                                                 >  1:3590342/1‑93 (MQ=255)
                        cTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAActgctg                                                                >  2:812583/1‑93 (MQ=255)
                         tGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTgg                                                               >  1:6154992/1‑93 (MQ=255)
                         tGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTgg                                                               >  1:3806615/1‑93 (MQ=255)
                           aCTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGaa                                                             >  2:1476581/1‑93 (MQ=255)
                           aCTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGaa                                                             <  1:1458122/93‑1 (MQ=255)
                            cTATCCCGCAAGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGaaa                                                            <  1:1905834/93‑1 (MQ=255)
                             tATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAAt                                                           <  2:3947851/93‑1 (MQ=255)
                             tATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAAt                                                           >  2:387432/1‑93 (MQ=255)
                                 ccGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCctc                                                       >  1:4869878/1‑93 (MQ=255)
                                  cGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCctct                                                      >  2:3611246/1‑93 (MQ=255)
                                   gCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTa                                                     >  2:2230887/1‑93 (MQ=255)
                                   gCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGAATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTa                                                     <  1:5623618/93‑1 (MQ=255)
                                     aGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAag                                                   <  1:5721108/93‑1 (MQ=255)
                                      ggAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAagc                                                  <  1:348953/93‑1 (MQ=255)
                                      ggAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAagc                                                  <  2:1362508/93‑1 (MQ=255)
                                      ggAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAagc                                                  >  1:3334434/1‑93 (MQ=255)
                                        aaCTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAagcag                                                <  2:2073151/93‑1 (MQ=255)
                                        aaCTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAagcag                                                >  2:5945174/1‑93 (MQ=255)
                                        aaCTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAagcag                                                <  1:4391021/93‑1 (MQ=255)
                                            ggCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCa                                            <  1:1012211/93‑1 (MQ=255)
                                            ggCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCa                                            <  2:4138341/93‑1 (MQ=255)
                                               atatGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTTGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTc                                         <  2:3089840/93‑1 (MQ=255)
                                                 atGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTg                                       <  2:2485885/93‑1 (MQ=255)
                                                  tGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGt                                      >  2:327678/1‑93 (MQ=255)
                                                   ggCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGtt                                     <  2:5365714/93‑1 (MQ=255)
                                                    gCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTc                                    <  2:2012481/93‑1 (MQ=255)
                                                    gCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTc                                    <  1:4272152/93‑1 (MQ=255)
                                                       aGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTcccc                                 <  1:4065372/93‑1 (MQ=255)
                                                        gcgcGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCt                                <  2:2858998/93‑1 (MQ=255)
                                                        gcgcGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCt                                >  1:5339670/1‑93 (MQ=255)
                                                        gcgcGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCt                                <  1:5150930/93‑1 (MQ=255)
                                                        gcgcGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCt                                >  1:642714/1‑93 (MQ=255)
                                                        gcgcGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCt                                <  2:2773308/93‑1 (MQ=255)
                                                        gcgcGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCt                                >  2:978847/1‑93 (MQ=255)
                                                         cgcgGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTc                               <  1:3984983/93‑1 (MQ=255)
                                                           cggcgCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGa                             >  2:923641/1‑93 (MQ=255)
                                                            ggcgcaggcgcaTCCATCCCTCCGTTCAGCGCCCTGGCGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGac                            >  1:2081903/1‑92 (MQ=255)
                                                             gcgcaggcgcaTCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAAc                           >  1:3055188/1‑93 (MQ=255)
                                                             gcgcaggcgcaTCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAAc                           >  1:3939822/1‑93 (MQ=255)
                                                                caggcgcaTCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACgag                        >  1:2749441/1‑93 (MQ=255)
                                                                 aggcgcaTCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACgaga                       <  1:121454/93‑1 (MQ=255)
                                                                  ggcgcaTCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGagag                      >  1:1558698/1‑93 (MQ=255)
                                                                  ggcgcaTCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGagag                      >  2:4561916/1‑93 (MQ=255)
                                                                   gcgcaTCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAgg                     <  1:3700285/93‑1 (MQ=255)
                                                                    cgcaTCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAggg                    <  1:2146700/93‑1 (MQ=255)
                                                                     gcaTCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAgggg                   <  1:766654/93‑1 (MQ=255)
                                                                     gcaTCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAgggg                   >  2:363105/1‑93 (MQ=255)
                                                                       atccatccCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAg                 >  2:5846451/1‑93 (MQ=255)
                                                                        tccatccCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGc                >  1:4993472/1‑93 (MQ=255)
                                                                        tccatccCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGc                <  2:279267/93‑1 (MQ=255)
                                                                        tccatccCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGc                >  1:815887/1‑93 (MQ=255)
                                                                          catccCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAg              >  2:1675853/1‑93 (MQ=255)
                                                                            tccCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAgga            >  1:3137455/1‑92 (MQ=255)
                                                                            tccCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGc            >  1:547665/1‑93 (MQ=255)
                                                                            tccCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGc            >  2:1763091/1‑93 (MQ=255)
                                                                              ccTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGTGCAGGCAt          >  2:1285758/1‑93 (MQ=255)
                                                                              ccTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCAt          >  1:1945835/1‑93 (MQ=255)
                                                                              ccTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCAt          >  2:1110551/1‑93 (MQ=255)
                                                                               cTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCAtt         <  1:1306303/93‑1 (MQ=255)
                                                                                 ccGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCa       >  2:3454487/1‑93 (MQ=255)
                                                                                 ccGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCa       <  2:3426498/93‑1 (MQ=255)
                                                                                  cGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAg      <  1:2356322/93‑1 (MQ=255)
                                                                                  cGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAg      >  1:3469427/1‑93 (MQ=255)
                                                                                   gTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGc     >  1:2547364/1‑93 (MQ=255)
                                                                                     tCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCaa   <  1:5751345/93‑1 (MQ=255)
                                                                                      cAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAAt  <  2:2946353/93‑1 (MQ=255)
                                                                                      cAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAAt  >  1:4654706/1‑93 (MQ=255)
                                                                                          |                                                                                        
ATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCACCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAAT  >  NC_000913/4427005‑4427183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCACCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAACC  >  NC_000913/4427002‑4427188
                                                                                             |                                                                                             
CTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCG                                                                                               <  SRR4302533.5735408/94‑1 (MQ=60)
CTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCG                                                                                               >  SRR4302533.5700014/1‑94 (MQ=60)
 TGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGC                                                                                              <  SRR4302533.5884962/94‑1 (MQ=60)
 TGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGC                                                                                              >  SRR4302533.4995296/1‑94 (MQ=60)
  GATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCC                                                                                             <  SRR4302533.4138445/94‑1 (MQ=60)
  GATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCC                                                                                             >  SRR4302533.852129/1‑94 (MQ=60)
    TGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCT                                                                                           <  SRR4302533.2041724/94‑1 (MQ=60)
    TGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCT                                                                                           <  SRR4302533.4311807/94‑1 (MQ=60)
       CGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGT                                                                                        <  SRR4302533.5115708/94‑1 (MQ=60)
       CGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGT                                                                                        >  SRR4302533.4222852/1‑94 (MQ=60)
        GGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTG                                                                                       >  SRR4302533.217008/1‑94 (MQ=60)
        GGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTG                                                                                       >  SRR4302533.6161079/1‑94 (MQ=60)
         GTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGT                                                                                      >  SRR4302533.455937/1‑94 (MQ=60)
         GTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGT                                                                                      <  SRR4302533.4512161/94‑1 (MQ=60)
         GTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGT                                                                                      <  SRR4302533.1156908/94‑1 (MQ=60)
          TAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTT                                                                                     >  SRR4302533.2909611/1‑94 (MQ=60)
          TAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTT                                                                                     <  SRR4302533.2495535/94‑1 (MQ=60)
           AGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTT                                                                                    <  SRR4302533.3761885/94‑1 (MQ=60)
           AGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTT                                                                                    >  SRR4302533.461335/1‑94 (MQ=60)
            GGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTG                                                                                   >  SRR4302533.614942/1‑94 (MQ=60)
            GGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTG                                                                                   >  SRR4302533.3155768/1‑94 (MQ=60)
             GTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGA                                                                                  <  SRR4302533.866715/94‑1 (MQ=60)
             GTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGA                                                                                  >  SRR4302533.2038048/1‑94 (MQ=60)
              TTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAA                                                                                 >  SRR4302533.3189032/1‑94 (MQ=60)
               TCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGTGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAG                                                                                <  SRR4302533.3401350/94‑1 (MQ=60)
               TCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAG                                                                                <  SRR4302533.2870687/94‑1 (MQ=60)
                CTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAAGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGT                                                                               <  SRR4302533.2658622/94‑1 (MQ=60)
                 TAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTC                                                                              <  SRR4302533.1140284/94‑1 (MQ=60)
                  AAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCG                                                                             >  SRR4302533.6070878/1‑94 (MQ=60)
                   AATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGA                                                                            >  SRR4302533.4678211/1‑94 (MQ=60)
                   AATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGA                                                                            >  SRR4302533.5686545/1‑94 (MQ=60)
                     TGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAAC                                                                          <  SRR4302533.2158615/94‑1 (MQ=60)
                      GGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACT                                                                         >  SRR4302533.1981586/1‑94 (MQ=60)
                       GGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTG                                                                        >  SRR4302533.5133354/1‑94 (MQ=60)
                       GGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTG                                                                        <  SRR4302533.1369020/94‑1 (MQ=60)
                        GAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGC                                                                       <  SRR4302533.5810948/94‑1 (MQ=60)
                         AACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCT                                                                      >  SRR4302533.3612443/1‑94 (MQ=60)
                         AACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCT                                                                      <  SRR4302533.2242847/94‑1 (MQ=60)
                          ACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTG                                                                     >  SRR4302533.779480/1‑94 (MQ=60)
                           CTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGG                                                                    >  SRR4302533.6192023/1‑94 (MQ=60)
                           CTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGG                                                                    <  SRR4302533.3387011/94‑1 (MQ=60)
                           CTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGG                                                                    >  SRR4302533.3829906/1‑94 (MQ=60)
                           CTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGG                                                                    >  SRR4302533.2839415/1‑94 (MQ=60)
                             GACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAA                                                                  <  SRR4302533.502605/94‑1 (MQ=60)
                              ACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAA                                                                 <  SRR4302533.1466913/94‑1 (MQ=60)
                              ACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAA                                                                 >  SRR4302533.5395914/1‑94 (MQ=60)
                              ACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAA                                                                 >  SRR4302533.907853/1‑94 (MQ=60)
                               CTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAAT                                                                <  SRR4302533.374151/94‑1 (MQ=60)
                               CTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAAT                                                                <  SRR4302533.4868123/94‑1 (MQ=60)
                               CTATCCCGCAAGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAAT                                                                <  SRR4302533.1917460/94‑1 (MQ=60)
                                TATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATC                                                               >  SRR4302533.4224084/1‑94 (MQ=60)
                                   CCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTC                                                            >  SRR4302533.4899386/1‑94 (MQ=60)
                                   CCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTC                                                            >  SRR4302533.4899385/1‑94 (MQ=60)
                                   CCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCACTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTC                                                            >  SRR4302533.5800402/1‑94 (MQ=60)
                                     CGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTA                                                          <  SRR4302533.3203859/94‑1 (MQ=60)
                                      GCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGAATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAA                                                         <  SRR4302533.5657609/94‑1 (MQ=60)
                                       CAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAG                                                        <  SRR4302533.4568656/94‑1 (MQ=60)
                                        AGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGC                                                       >  SRR4302533.3355038/1‑94 (MQ=60)
                                        AGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGC                                                       <  SRR4302533.5755651/94‑1 (MQ=60)
                                         GGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCA                                                      <  SRR4302533.351083/94‑1 (MQ=60)
                                          GAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAG                                                     >  SRR4302533.3764492/1‑94 (MQ=60)
                                           AACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGC                                                    <  SRR4302533.350812/94‑1 (MQ=60)
                                           AACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGC                                                    <  SRR4302533.4417529/94‑1 (MQ=60)
                                            ACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCG                                                   >  SRR4302533.3508548/1‑94 (MQ=60)
                                             CTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGC                                                  >  SRR4302533.3730879/1‑94 (MQ=60)
                                             CTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGC                                                  >  SRR4302533.74143/1‑94 (MQ=60)
                                             CTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGC                                                  <  SRR4302533.4044726/94‑1 (MQ=60)
                                              TGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCA                                                 <  SRR4302533.5250439/94‑1 (MQ=60)
                                              TGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCA                                                 >  SRR4302533.2598045/1‑94 (MQ=60)
                                               GGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCAT                                                <  SRR4302533.1018134/94‑1 (MQ=60)
                                                GCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATT                                               <  SRR4302533.1513643/94‑1 (MQ=60)
                                                 CATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCGGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTC                                              >  SRR4302533.6342769/1‑94 (MQ=60)
                                                 CATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTC                                              <  SRR4302533.4492254/94‑1 (MQ=60)
                                                 CATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTC                                              >  SRR4302533.5353626/1‑94 (MQ=60)
                                                  ATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCT                                             <  SRR4302533.2288491/94‑1 (MQ=60)
                                                    ATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGT                                           <  SRR4302533.3276115/94‑1 (MQ=60)
                                                     TGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTT                                          >  SRR4302533.3065285/1‑94 (MQ=60)
                                                      GGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCTCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCGGTTC                                         <  SRR4302533.2399898/94‑1 (MQ=60)
                                                       GCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCC                                        <  SRR4302533.5645903/94‑1 (MQ=60)
                                                       GCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCC                                        <  SRR4302533.4297864/94‑1 (MQ=60)
                                                       GCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCC                                        <  SRR4302533.4008732/94‑1 (MQ=60)
                                                        CGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCC                                       >  SRR4302533.5667192/1‑94 (MQ=60)
                                                        CGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCAAACTGCTGGAAATCCTCTAACCAGCGCATTCTGTTCCC                                       >  SRR4302533.5403755/1‑94 (MQ=60)
                                                         GAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCC                                      <  SRR4302533.3885652/94‑1 (MQ=60)
                                                          AGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCT                                     <  SRR4302533.4090120/94‑1 (MQ=60)
                                                          AGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCT                                     >  SRR4302533.646491/1‑94 (MQ=60)
                                                          AGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCT                                     >  SRR4302533.5371691/1‑94 (MQ=60)
                                                          AGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCT                                     <  SRR4302533.3550894/94‑1 (MQ=60)
                                                           GCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTC                                    <  SRR4302533.5182046/94‑1 (MQ=60)
                                                           GCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTC                                    <  SRR4302533.682333/94‑1 (MQ=60)
                                                           GCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTC                                    <  SRR4302533.970100/94‑1 (MQ=60)
                                                           GCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTC                                    <  SRR4302533.2267652/94‑1 (MQ=60)
                                                           GCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTC                                    <  SRR4302533.2909310/94‑1 (MQ=60)
                                                           GCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTC                                    <  SRR4302533.1256112/94‑1 (MQ=60)
                                                            CGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCG                                   <  SRR4302533.4009245/94‑1 (MQ=60)
                                                              CGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGGGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAA                                 >  SRR4302533.1776297/1‑94 (MQ=60)
                                                              CGGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGCGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAC                                 >  SRR4302533.2094716/1‑94 (MQ=60)
                                                               GGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAAC                                >  SRR4302533.4138448/1‑94 (MQ=60)
                                                               GGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAAC                                >  SRR4302533.3073915/1‑94 (MQ=60)
                                                               GGCGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAAC                                >  SRR4302533.3963864/1‑94 (MQ=60)
                                                                 CGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGA                              >  SRR4302533.1939663/1‑94 (MQ=60)
                                                                 CGCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGA                              <  SRR4302533.3973465/94‑1 (MQ=60)
                                                                  GCAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAG                             >  SRR4302533.2766297/1‑94 (MQ=60)
                                                                   CAGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGA                            >  SRR4302533.17639/1‑94 (MQ=60)
                                                                    AGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAG                           >  SRR4302533.1568084/1‑94 (MQ=60)
                                                                    AGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAG                           <  SRR4302533.122221/94‑1 (MQ=60)
                                                                    AGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAG                           <  SRR4302533.6090155/94‑1 (MQ=60)
                                                                    AGGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAG                           >  SRR4302533.4286304/1‑94 (MQ=60)
                                                                     GGCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGG                          <  SRR4302533.1254718/94‑1 (MQ=60)
                                                                      GCGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGG                         <  SRR4302533.3723012/94‑1 (MQ=60)
                                                                       CGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGG                        >  SRR4302533.1969615/1‑94 (MQ=60)
                                                                       CGCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGG                        <  SRR4302533.2159922/94‑1 (MQ=60)
                                                                        GCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGA                       <  SRR4302533.771178/94‑1 (MQ=60)
                                                                        GCATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGA                       <  SRR4302533.1079806/94‑1 (MQ=60)
                                                                         CATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAG                      <  SRR4302533.1209643/94‑1 (MQ=60)
                                                                          ATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGC                     >  SRR4302533.820670/1‑94 (MQ=60)
                                                                          ATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGC                     >  SRR4302533.5023746/1‑94 (MQ=60)
                                                                          ATCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGC                     >  SRR4302533.3012289/1‑94 (MQ=60)
                                                                           TCCATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCA                    <  SRR4302533.3613365/94‑1 (MQ=60)
                                                                              ATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGC                 >  SRR4302533.550916/1‑94 (MQ=60)
                                                                              ATCCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGA                 >  SRR4302533.3156745/1‑94 (MQ=60)
                                                                                CCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCAT               >  SRR4302533.1957699/1‑94 (MQ=60)
                                                                                CCCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCAT               >  SRR4302533.3431102/1‑94 (MQ=60)
                                                                                 CCTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATT              >  SRR4302533.2557515/1‑94 (MQ=60)
                                                                                  CTCCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTC             <  SRR4302533.1314151/94‑1 (MQ=60)
                                                                                    CCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAG           >  SRR4302533.4982801/1‑94 (MQ=60)
                                                                                    CCGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAG           >  SRR4302533.3490844/1‑94 (MQ=60)
                                                                                     CGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGC          <  SRR4302533.2370890/94‑1 (MQ=60)
                                                                                     CGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGC          <  SRR4302533.1156668/94‑1 (MQ=60)
                                                                                     CGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGC          >  SRR4302533.2563042/1‑94 (MQ=60)
                                                                                     CGTTCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGC          <  SRR4302533.5758314/94‑1 (MQ=60)
                                                                                        TCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAAT       <  SRR4302533.5786041/94‑1 (MQ=60)
                                                                                        TCAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAAT       >  SRR4302533.4682911/1‑94 (MQ=60)
                                                                                         CAGCGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATA      <  SRR4302533.3166822/94‑1 (MQ=60)
                                                                                            CGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAAC   <  SRR4302533.1309287/94‑1 (MQ=60)
                                                                                            CGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAAC   <  SRR4302533.2625860/94‑1 (MQ=60)
                                                                                            CGCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAAC   <  SRR4302533.2453212/94‑1 (MQ=60)
                                                                                             GCCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAACC  >  SRR4302533.3742196/1‑94 (MQ=60)
                                                                                             |                                                                                             
CTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCACCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAACC  >  NC_000913/4427002‑4427188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑