Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 245 579.6 28867798 97.7% 28203838 96.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,007,718 2 bp→CG coding (943‑944/1023 nt) pldB → lysophospholipase L(2)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,007,7180GC99.6% 1757.5 / NA 444A315R (GCC→CGC) pldBlysophospholipase L(2)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (222/220);  minor base A (2/0);  total (224/220)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009134,007,7190CG99.8% 1765.4 / NA 445A315R (GCC→CGC) pldBlysophospholipase L(2)
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (224/220);  total (224/221)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 460 total aligned reads displayed.

CGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGGCCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTATG  >  NC_000913/4007628‑4007808
                                                                                          ||                                                                                         
cGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCa                                                                                       >  2:10998048/1‑96 (MQ=255)
cGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCa                                                                                       <  2:8018434/96‑1 (MQ=255)
 gCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAg                                                                                      >  2:13863092/1‑96 (MQ=255)
  cACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGt                                                                                     >  2:7522375/1‑96 (MQ=255)
   cccgccgCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTc                                                                                    <  1:1685530/95‑1 (MQ=255)
   atcgccgcGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTc                                                                                    >  1:10679985/3‑96 (MQ=255)
   aCCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTc                                                                                    <  1:9397978/96‑1 (MQ=255)
   aCCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACAATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTc                                                                                    <  2:1547243/96‑1 (MQ=255)
    ccgccgCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTcg                                                                                   <  2:488847/96‑1 (MQ=255)
    ccgccgCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTACTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTcg                                                                                   <  2:12886689/96‑1 (MQ=255)
      gccgcGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTcgcg                                                                                 >  2:8589726/1‑96 (MQ=255)
      gccgcGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTcgcg                                                                                 >  2:9549966/1‑96 (MQ=255)
      gccgcGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTagcg                                                                                 >  1:5662461/1‑96 (MQ=255)
          cgGGCCATCCCGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTcc                                                                             <  1:6324870/96‑1 (MQ=255)
            ggCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCaa                                                                           <  1:13830124/96‑2 (MQ=255)
            ggCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCAc                                                                           <  2:13443687/96‑1 (MQ=255)
            ggCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCAc                                                                           <  2:7036624/96‑1 (MQ=255)
            ggCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCAc                                                                           <  1:3246123/96‑1 (MQ=255)
             gCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACg                                                                          >  1:4357824/1‑96 (MQ=255)
             gCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACg                                                                          <  2:2748095/96‑1 (MQ=255)
               cATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGcc                                                                        >  2:169259/1‑96 (MQ=255)
               cATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGcc                                                                        >  1:284472/1‑96 (MQ=255)
                 tCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGccct                                                                      <  1:7748212/96‑3 (MQ=255)
                      tCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTg                                                                 >  1:8708316/1‑96 (MQ=255)
                        gAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGAt                                                               >  2:4231310/1‑96 (MQ=255)
                         aaGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGAtt                                                              <  1:11155877/96‑1 (MQ=255)
                         aaGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGAtt                                                              >  1:8040438/1‑96 (MQ=255)
                         aaGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAAGGCGCTCAGTCGCGCTCCACGCCATCGTTGAtt                                                              >  1:679118/1‑96 (MQ=255)
                          aggaggACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGAttt                                                             <  1:13546332/96‑1 (MQ=255)
                          aggaggACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGAttt                                                             >  2:8500376/1‑96 (MQ=255)
                           ggaggaCGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGAtttt                                                            <  1:1803612/96‑1 (MQ=255)
                            gaggaCGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGAttttt                                                           >  1:9625806/1‑96 (MQ=255)
                             aggaCGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGAtgtttt                                                          >  2:7637339/1‑96 (MQ=255)
                              ggaCGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTc                                                         >  2:12054100/1‑96 (MQ=255)
                               gaCGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCa                                                        >  1:10026349/1‑96 (MQ=255)
                                aCGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCaa                                                       >  1:2102454/1‑96 (MQ=255)
                                aCGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCaa                                                       >  2:3797891/1‑96 (MQ=255)
                                 cGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAAc                                                      <  1:4780030/96‑1 (MQ=255)
                                  ggCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACa                                                     <  1:3619777/96‑1 (MQ=255)
                                    ccGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAgg                                                   <  2:6642559/96‑1 (MQ=255)
                                    ccGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAgg                                                   >  1:7644355/1‑96 (MQ=255)
                                    ccGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAgg                                                   >  2:11718422/1‑96 (MQ=255)
                                    ccGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAgg                                                   >  1:4526594/1‑96 (MQ=255)
                                       ttGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCAt                                                >  1:6081120/1‑96 (MQ=255)
                                        tGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATa                                               >  2:6151387/1‑96 (MQ=255)
                                        tGGTAATTAAAGGGGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATa                                               >  2:3019515/1‑96 (MQ=255)
                                         ggTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATaa                                              <  1:8527954/96‑1 (MQ=255)
                                         ggTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATaa                                              <  1:9955610/96‑1 (MQ=255)
                                          gTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATatc                                             <  1:14315224/96‑3 (MQ=255)
                                          gTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAAc                                             >  1:4219104/1‑96 (MQ=255)
                                          gTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAAc                                             >  2:10261013/1‑96 (MQ=255)
                                          gTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAAc                                             >  2:221044/1‑96 (MQ=255)
                                            aaTTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTc                                           >  2:11115340/1‑96 (MQ=255)
                                             aTTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCa                                          >  2:4747315/1‑96 (MQ=255)
                                             aTTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCa                                          <  2:14057405/96‑1 (MQ=255)
                                              ttAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCAc                                         >  2:13551856/1‑96 (MQ=255)
                                              ttAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCAc                                         >  2:7339787/1‑96 (MQ=255)
                                               tAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCAcc                                        >  1:2481469/1‑96 (MQ=255)
                                                    gTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCg                                   <  2:9640629/96‑1 (MQ=255)
                                                     tGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCgg                                  <  1:1516412/96‑1 (MQ=255)
                                                      gCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGa                                 >  1:5157411/1‑96 (MQ=255)
                                                       cTTGCCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGaa                                <  1:3508135/96‑1 (MQ=255)
                                                        ttACCATGAGATCCTTTTTGAAAAGGACGCCATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGaaa                               <  1:6950971/96‑1 (MQ=255)
                                                          aCCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAAcc                             <  1:6190765/96‑1 (MQ=255)
                                                           ccATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCg                            >  1:8136051/1‑96 (MQ=255)
                                                             aTGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGct                          >  2:12297567/1‑96 (MQ=255)
                                                                agaTCCTTTTTGAAAAGGACGCAGTGCGCTCAGTCGCGCTCCACGCCGTCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTa                       <  1:9755254/96‑1 (MQ=255)
                                                                agaTCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCCCCCAGCGGAAACCGCTCTa                       <  2:8132504/96‑1 (MQ=255)
                                                                agaTCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTa                       >  2:1644525/1‑96 (MQ=255)
                                                                agaTCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTa                       <  2:4523540/96‑1 (MQ=255)
                                                                 gaTCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTAc                      <  1:3013315/96‑1 (MQ=255)
                                                                 gaTCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTAc                      <  1:920838/96‑1 (MQ=255)
                                                                 gaTCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTAc                      <  2:3231484/96‑1 (MQ=255)
                                                                    cttttttGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCGCCCAGCGGAAACCGCTCTACaga                   >  1:10158366/3‑96 (MQ=255)
                                                                    ccTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACaga                   <  2:12695099/96‑1 (MQ=255)
                                                                     cTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACagag                  <  1:12502818/96‑1 (MQ=255)
                                                                      tttttGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAgg                 <  2:9191989/96‑1 (MQ=255)
                                                                      tttttGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAgg                 >  2:12420419/1‑96 (MQ=255)
                                                                        tttGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGtt               <  1:14005693/96‑1 (MQ=255)
                                                                        tttGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGtt               >  2:8935235/1‑96 (MQ=255)
                                                                         ttGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGttt              >  2:534431/1‑96 (MQ=255)
                                                                         ttGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGttt              <  2:5201169/96‑1 (MQ=255)
                                                                         ttGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGttt              <  2:4136468/96‑1 (MQ=255)
                                                                          tGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTa             >  1:12008784/1‑96 (MQ=255)
                                                                           gAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTaa            >  2:5825202/1‑96 (MQ=255)
                                                                           gAAAAGGACGCAATACGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTaa            <  1:12793492/96‑1 (MQ=255)
                                                                              aaGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAAtt         <  2:14350589/96‑1 (MQ=255)
                                                                              aaGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAAtt         <  1:13636803/96‑1 (MQ=255)
                                                                               aGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAAttt        >  1:11731731/1‑96 (MQ=255)
                                                                               aGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAAttt        <  2:3436327/96‑1 (MQ=255)
                                                                                ggACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTc       <  1:8320425/96‑1 (MQ=255)
                                                                                ggACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTCTAAATTTc       <  1:12946883/96‑1 (MQ=255)
                                                                                 gACGCAATGCGCTCAGTCGCGCTCCACGCCCTCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCt      <  2:3726433/96‑1 (MQ=255)
                                                                                  aCGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCtt     <  2:1397956/96‑1 (MQ=255)
                                                                                   cGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTa    >  1:7307830/1‑96 (MQ=255)
                                                                                    gCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTAt   >  1:1048301/1‑96 (MQ=255)
                                                                                    gCAATGAGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTAt   >  2:13698000/1‑96 (MQ=255)
                                                                                     cAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTATg  <  2:5594853/96‑1 (MQ=255)
                                                                                     cAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTATg  >  2:11919458/1‑96 (MQ=255)
                                                                                     cAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTATg  <  2:2650472/96‑1 (MQ=255)
                                                                                          ||                                                                                         
CGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGGCCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTATG  >  NC_000913/4007628‑4007808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 240 total aligned reads displayed.
TTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGGCCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTATGTACCAG  >  NC_000913/4007618‑4007814
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TTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGC                                                                                                  <  SRR4302518.6837645/101‑1 (MQ=60)
TTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGC                                                                                                  <  SRR4302518.772678/101‑1 (MQ=60)
TTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAANNN                                                                                                  >  SRR4302518.14129182/1‑101 (MQ=60)
TCGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAANNN                                                                                                  >  SRR4302518.9066918/1‑101 (MQ=60)
NNNTGATCTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGC                                                                                                  <  SRR4302518.13491978/101‑1 (MQ=60)
NNNTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGACAAGGACGCAATGC                                                                                                  <  SRR4302518.11520492/101‑1 (MQ=60)
      ACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAG                                                                                            <  SRR4302518.4624477/101‑1 (MQ=60)
      ACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAG                                                                                            <  SRR4302518.732439/101‑1 (MQ=60)
        TCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTC                                                                                          >  SRR4302518.4337234/1‑101 (MQ=60)
        NNNGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTC                                                                                          <  SRR4302518.9852431/101‑1 (MQ=60)
        CCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTC                                                                                          <  SRR4302518.6495233/101‑1 (MQ=60)
         CCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCG                                                                                         <  SRR4302518.1632260/101‑1 (MQ=60)
          CGCACCGCCGCGGGCCATCCTGTCGAAGGAGGCGGGCGGTTGGTAGTTAAAGGGGCTTACCATGAGATCATTTTTGAAAAGGACGCAATGCGCTCAGTCGC                                                                                        >  SRR4302518.4752240/1‑101 (MQ=60)
          CGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGC                                                                                        <  SRR4302518.6589556/101‑1 (MQ=60)
          CGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGC                                                                                        <  SRR4302518.1764124/101‑1 (MQ=60)
           GCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACGATGAGGTCCTTTTTGAAAAGGACGCAATGCGCTCAGTCNNN                                                                                       >  SRR4302518.13308753/1‑101 (MQ=60)
           GCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCG                                                                                       >  SRR4302518.788230/1‑101 (MQ=60)
           GAACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGCTCCTTTTTGAAAAGGACGCAATGCGCTCAGTCNNN                                                                                       >  SRR4302518.11179801/1‑101 (MQ=60)
            NNNCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGC                                                                                      <  SRR4302518.10125218/101‑1 (MQ=60)
            CCCCGCCGCGGGCCATCCTGTCGAAGGAGGACGGGCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGC                                                                                      <  SRR4302518.5645098/101‑1 (MQ=60)
             ATCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCNNN                                                                                     >  SRR4302518.11181696/1‑101 (MQ=60)
             ACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCNNN                                                                                     >  SRR4302518.8040270/1‑101 (MQ=60)
              NNNCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTC                                                                                    <  SRR4302518.13596853/101‑1 (MQ=60)
               CGCCGCGGGCCATCCCGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCC                                                                                   <  SRR4302518.6647402/101‑1 (MQ=60)
               CGACGCGGGACATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCC                                                                                   <  SRR4302518.6504340/101‑1 (MQ=60)
                GCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCA                                                                                  >  SRR4302518.3846530/1‑101 (MQ=60)
                GCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTAGCGCTCCA                                                                                  >  SRR4302518.5949923/1‑101 (MQ=60)
                GCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGACGCGCTNNN                                                                                  >  SRR4302518.8483507/1‑101 (MQ=60)
                GCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCGCAGTCGCGCTNNN                                                                                  >  SRR4302518.9944332/1‑101 (MQ=60)
                 NNNCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCTC                                                                                 <  SRR4302518.9861716/101‑1 (MQ=60)
                 NNNCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCAC                                                                                 <  SRR4302518.14193010/101‑1 (MQ=60)
                 NNNCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCAA                                                                                 <  SRR4302518.14463128/101‑1 (MQ=60)
                 CCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCAC                                                                                 <  SRR4302518.3396965/101‑1 (MQ=60)
                 CCGCGGGACATCCTGTCGAAGGAGGACGGCCATTGGTAATTAAACGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCAC                                                                                 <  SRR4302518.1112402/101‑1 (MQ=60)
                  NNNGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACG                                                                                <  SRR4302518.11834630/101‑1 (MQ=60)
                  CGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACG                                                                                >  SRR4302518.2720612/1‑101 (MQ=60)
                   GCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGC                                                                               <  SRR4302518.3001306/101‑1 (MQ=60)
                   GCGGCCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGC                                                                               <  SRR4302518.1326037/101‑1 (MQ=60)
                    CGGGCCATCCTGTCGAAGGAGGACTGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCC                                                                              <  SRR4302518.82189/101‑1 (MQ=60)
                    CGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACNNN                                                                              >  SRR4302518.14199810/1‑101 (MQ=60)
                    CGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCC                                                                              <  SRR4302518.1076671/101‑1 (MQ=60)
                     GGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGNNN                                                                             >  SRR4302518.10139486/1‑101 (MQ=60)
                     GGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCGA                                                                             >  SRR4302518.2392183/1‑101 (MQ=60)
                     GGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCA                                                                             <  SRR4302518.4697941/101‑1 (MQ=60)
                     GGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCA                                                                             <  SRR4302518.3536189/101‑1 (MQ=60)
                      NNNCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCCT                                                                            <  SRR4302518.8140582/101‑1 (MQ=60)
                       NNNATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATG                                                                           <  SRR4302518.15018983/101‑1 (MQ=60)
                       NNNATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATC                                                                           <  SRR4302518.9347010/101‑1 (MQ=60)
                       GCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATG                                                                           >  SRR4302518.4576313/1‑101 (MQ=60)
                       GCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATC                                                                           <  SRR4302518.5326429/101‑1 (MQ=60)
                        CCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCG                                                                          >  SRR4302518.4323655/1‑101 (MQ=60)
                        CCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCANNN                                                                          >  SRR4302518.10421336/1‑101 (MQ=60)
                         CATCCTGTCGAAGGAGGACGGCCGTTTGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGCCGCACTGCGCTCAGTCGCGCTCCACGCCCTCGT                                                                         <  SRR4302518.3787005/101‑1 (MQ=60)
                         CATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGT                                                                         >  SRR4302518.295936/1‑101 (MQ=60)
                          NNNCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTT                                                                        <  SRR4302518.8599612/101‑1 (MQ=60)
                              nnngGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATT                                                                    <  SRR4302518.11678144/97‑1 (MQ=60)
                               NNNGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTT                                                                   <  SRR4302518.14168112/101‑1 (MQ=60)
                                TCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTT                                                                  >  SRR4302518.3645361/1‑101 (MQ=60)
                                TCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATNNN                                                                  >  SRR4302518.9131672/1‑101 (MQ=60)
                                TCGAAGGAGGACGGCCGTTGGGAATTAGAGGTGCTTACCATGAGATCCTTTTTGAAAAGGCCGCAATGCGCTCAGGCGCGCTCCACGCCATCGTTGATNNN                                                                  >  SRR4302518.10167738/1‑101 (MQ=60)
                                TCGAAGGAGGACGACCGTTGGAAATTAAAGGTGCTTACCATGAGATACTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATNNN                                                                  >  SRR4302518.14505974/1‑101 (MQ=60)
                                NNNAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATGCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTCT                                                                  <  SRR4302518.9452740/101‑1 (MQ=60)
                                NNNAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTT                                                                  <  SRR4302518.7816438/101‑1 (MQ=60)
                                GCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTT                                                                  <  SRR4302518.1884600/101‑1 (MQ=60)
                                 CGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTT                                                                 >  SRR4302518.5468864/1‑101 (MQ=60)
                                  GAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAGAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTNNN                                                                >  SRR4302518.8368145/1‑101 (MQ=60)
                                   AAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTNNN                                                               >  SRR4302518.8440753/1‑101 (MQ=60)
                                   AAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAAGGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTC                                                               >  SRR4302518.705507/1‑101 (MQ=60)
                                    NNNAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCA                                                              <  SRR4302518.8078846/101‑1 (MQ=60)
                                    AGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCA                                                              >  SRR4302518.88098/1‑101 (MQ=60)
                                    AGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCA                                                              >  SRR4302518.1767772/1‑101 (MQ=60)
                                      NNNGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACTCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAAC                                                            <  SRR4302518.13623020/101‑1 (MQ=60)
                                      GAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCNNN                                                            >  SRR4302518.10090296/1‑101 (MQ=60)
                                      GAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAAC                                                            <  SRR4302518.5030412/101‑1 (MQ=60)
                                      GAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAAC                                                            >  SRR4302518.1253240/1‑101 (MQ=60)
                                       AGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCANNN                                                           >  SRR4302518.8455697/1‑101 (MQ=60)
                                       AGGACGGCCGTTGGTAATTAAAGGGGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTaannn                                                           >  SRR4302518.7673311/1‑96 (MQ=60)
                                        NNNCGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAG                                                          <  SRR4302518.7670653/101‑1 (MQ=60)
                                        GGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAANNN                                                          >  SRR4302518.8138539/1‑101 (MQ=60)
                                        GGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAG                                                          <  SRR4302518.2049902/101‑1 (MQ=60)
                                         GACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGG                                                         >  SRR4302518.3734536/1‑101 (MQ=60)
                                          ACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGC                                                        >  SRR4302518.2201134/1‑101 (MQ=60)
                                           CGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGNNN                                                       >  SRR4302518.14810367/1‑101 (MQ=60)
                                            GTCCGTGGGTAATTGAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGAAATGCGCTCAGTCGCTGTCCACGCCATCGTTGATTTTTTCAACAGGCAT                                                      <  SRR4302518.7213838/101‑1 (MQ=60)
                                             NNNGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATA                                                     <  SRR4302518.8703192/101‑1 (MQ=60)
                                             GCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATA                                                     >  SRR4302518.1759286/1‑101 (MQ=60)
                                              NNNTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAA                                                    <  SRR4302518.10429723/101‑1 (MQ=60)
                                              NNNTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAA                                                    <  SRR4302518.8944070/101‑1 (MQ=60)
                                              NNNTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACACAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAA                                                    <  SRR4302518.9794241/101‑1 (MQ=60)
                                              CCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAA                                                    >  SRR4302518.4763863/1‑101 (MQ=60)
                                               NNNTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATATC                                                   <  SRR4302518.14977245/101‑1 (MQ=60)
                                               CGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATNNN                                                   >  SRR4302518.14383905/1‑101 (MQ=60)
                                               CGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATNNN                                                   >  SRR4302518.14378349/1‑101 (MQ=60)
                                               CGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAAC                                                   >  SRR4302518.2505012/1‑101 (MQ=60)
                                                GTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCAgannn                                                  >  SRR4302518.11602710/1‑96 (MQ=60)
                                                GTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATANNN                                                  >  SRR4302518.10980124/1‑101 (MQ=60)
                                                GTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACT                                                  >  SRR4302518.6507768/1‑101 (MQ=60)
                                                GTTGGTAATTAAAGGTGCTCCCCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACT                                                  <  SRR4302518.3130267/101‑1 (MQ=60)
                                                 nnngtaatgaagggtgcttagcatgtgttttttttaaAAAAGGACGCAATGCGCTCAGCCGCCCTCCCCGCCATCGTTGATTTTTTCAACAGGCATAACAC                                                 <  SRR4302518.14939071/64‑1 (MQ=40)
                                                 TTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTC                                                 >  SRR4302518.6389785/1‑101 (MQ=60)
                                                 TTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTC                                                 >  SRR4302518.4635203/1‑101 (MQ=60)
                                                 NNNGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTC                                                 <  SRR4302518.13783571/101‑1 (MQ=60)
                                                    NNNATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACC                                              <  SRR4302518.10169630/101‑1 (MQ=60)
                                                    GTAATTAAAGGTGCTTACCATGAGATCTTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCCCGCCATCGTTGATTTTTTCAACAGGCATAACTCACC                                              <  SRR4302518.833872/101‑1 (MQ=60)
                                                    GTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACC                                              <  SRR4302518.282312/101‑1 (MQ=60)
                                                     NNNTTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCC                                             <  SRR4302518.12456830/101‑1 (MQ=60)
                                                      AATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCA                                            >  SRR4302518.1884494/1‑101 (MQ=60)
                                                      AATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCA                                            >  SRR4302518.5878637/1‑101 (MQ=60)
                                                       NNNAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAG                                           <  SRR4302518.10454913/101‑1 (MQ=60)
                                                       ATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCNNN                                           >  SRR4302518.7807553/1‑101 (MQ=60)
                                                         TAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCG                                         <  SRR4302518.3713345/101‑1 (MQ=60)
                                                         TAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCG                                         >  SRR4302518.2601109/1‑101 (MQ=60)
                                                         TAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCG                                         <  SRR4302518.4894750/101‑1 (MQ=60)
                                                          AAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGG                                        <  SRR4302518.4395138/101‑1 (MQ=60)
                                                          AAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGG                                        <  SRR4302518.1581405/101‑1 (MQ=60)
                                                           AAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGA‑TTTTTCAACAGGCATAACTCACCCAGCGGCA                                      >  SRR4302518.887938/1‑101 (MQ=60)
                                                            AGGTGCTTGCCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAA                                      <  SRR4302518.3671449/101‑1 (MQ=60)
                                                            AGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCCCCCAGCGGAA                                      <  SRR4302518.6263559/101‑1 (MQ=60)
                                                             GGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCCATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAA                                     <  SRR4302518.7320206/101‑1 (MQ=60)
                                                             GGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGGTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGNNN                                     >  SRR4302518.8302843/1‑101 (MQ=60)
                                                               TGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAANNN                                   >  SRR4302518.14227335/1‑101 (MQ=60)
                                                               TGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACC                                   >  SRR4302518.6174676/1‑101 (MQ=60)
                                                               TGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACC                                   >  SRR4302518.6001042/1‑101 (MQ=60)
                                                               NNNTTACCATGAGTTCCTTTTTGAAAAGGGCGCAATGCGCTCAGTCCCCCTCCCCGCCATCGTTGATTTTTTCAACAGGCATAACTCCCCCAGCGGAAACC                                   <  SRR4302518.14506214/101‑1 (MQ=60)
                                                               GGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACC                                   <  SRR4302518.6506447/101‑1 (MQ=60)
                                                                NNNTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCG                                  <  SRR4302518.7908645/101‑1 (MQ=60)
                                                                GCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCG                                  >  SRR4302518.5423561/1‑101 (MQ=60)
                                                                 CTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACNNN                                 >  SRR4302518.13438123/1‑101 (MQ=60)
                                                                 CTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACNNN                                 >  SRR4302518.10234896/1‑101 (MQ=60)
                                                                 CTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGC                                 <  SRR4302518.1288327/101‑1 (MQ=60)
                                                                 CTTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGC                                 >  SRR4302518.1840834/1‑101 (MQ=60)
                                                                  NNNCCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCT                                <  SRR4302518.9733307/101‑1 (MQ=60)
                                                                   TACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGNNN                               >  SRR4302518.15014979/1‑101 (MQ=60)
                                                                   NNNCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATACCTCACCCAGCGGAAACCGCTC                               <  SRR4302518.11513014/101‑1 (MQ=60)
                                                                    TCCATGATATCCTTTTTGAAAAGGACGCAATGCGCTAAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACCCACCCAGCGGAAACCGCTCT                              <  SRR4302518.6694315/101‑1 (MQ=60)
                                                                     CCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTNNN                             >  SRR4302518.13519314/1‑101 (MQ=60)
                                                                     CCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTNNN                             >  SRR4302518.8539580/1‑101 (MQ=60)
                                                                     CCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTA                             >  SRR4302518.1485972/1‑101 (MQ=60)
                                                                     ACATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGGCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTNNN                             >  SRR4302518.14389017/1‑101 (MQ=60)
                                                                      nnngacacCCTTTTGGAAAAGGACGAGATGCCCTTAGTAGCGCTCAACGGCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTAC                            <  SRR4302518.9819703/93‑1 (MQ=60)
                                                                      CATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTAC                            >  SRR4302518.5339414/1‑101 (MQ=60)
                                                                      CATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTAC                            <  SRR4302518.956423/101‑1 (MQ=60)
                                                                      CATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTAC                            <  SRR4302518.1200651/101‑1 (MQ=60)
                                                                      CATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTAC                            <  SRR4302518.3154654/101‑1 (MQ=60)
                                                                      CATGAGATCCTTTTTGAAAAGGACGCAATGCGCACAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCAAGCCGAAAACGATCTAC                            >  SRR4302518.7287235/1‑101 (MQ=60)
                                                                       ATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACA                           <  SRR4302518.4511496/101‑1 (MQ=60)
                                                                       ATGAGATCCTTTTTAAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACA                           <  SRR4302518.5112862/101‑1 (MQ=60)
                                                                        TGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAG                          <  SRR4302518.704436/101‑1 (MQ=60)
                                                                        TGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAG                          >  SRR4302518.2087697/1‑101 (MQ=60)
                                                                        TGAGATCCTTTTTGAAAACGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGCTGATTTTTTCAACAGGCATAACTCACCCAGCGGAACCCGCTCTACAG                          <  SRR4302518.7270655/101‑1 (MQ=60)
                                                                         GAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGA                         <  SRR4302518.641812/101‑1 (MQ=60)
                                                                          NNNTCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAG                        <  SRR4302518.13090314/101‑1 (MQ=60)
                                                                          AGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAG                        >  SRR4302518.4479742/1‑101 (MQ=60)
                                                                           GCTCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGG                       >  SRR4302518.3684458/1‑101 (MQ=60)
                                                                           GATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGG                       >  SRR4302518.715637/1‑101 (MQ=60)
                                                                            ATCCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCGCTACAGAGGT                      >  SRR4302518.1221056/1‑101 (MQ=60)
                                                                             NNNTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTT                     <  SRR4302518.9000885/101‑1 (MQ=60)
                                                                             NNNTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTT                     <  SRR4302518.14646522/101‑1 (MQ=60)
                                                                             NNNTTTTTGAAAAGGACGCAATACGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTT                     <  SRR4302518.14636417/101‑1 (MQ=60)
                                                                              CTTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCATGCCATCGTTGATTTTTTCAACAGGCATAACTCGCCCAGCGGAAACCGCTCTACAGAGGNNN                    >  SRR4302518.10638632/1‑101 (MQ=60)
                                                                              CCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGNNN                    >  SRR4302518.12965318/1‑101 (MQ=60)
                                                                              CCTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACACAGCGGAAACCGCTCTACAGAGGNNN                    >  SRR4302518.8810114/1‑101 (MQ=60)
                                                                              CCTTTTTGAAAACGACGAAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTT                    <  SRR4302518.4284498/101‑1 (MQ=60)
                                                                              CCTTGTTGAAAAGGACGCGATGCGCTCAGTCGCGCTCCAGGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGNNN                    >  SRR4302518.10617849/1‑101 (MQ=60)
                                                                               CTTTTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTA                   >  SRR4302518.160419/1‑101 (MQ=60)
                                                                                NNNTTGAAAAGGACGCAATACGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAA                  <  SRR4302518.13389279/101‑1 (MQ=60)
                                                                                  TTTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAAT                >  SRR4302518.3274082/1‑101 (MQ=60)
                                                                                  NNNGAAAAGGACGCAATGCGCTCAGTCGAGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAAT                <  SRR4302518.10534922/101‑1 (MQ=60)
                                                                                  NNNGAAAAGGACGCAATGCGCTCAGTCGAGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAAT                <  SRR4302518.10559495/101‑1 (MQ=60)
                                                                                   TTGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAANNN               >  SRR4302518.9099244/1‑101 (MQ=60)
                                                                                    TGAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAANNN              >  SRR4302518.12581017/1‑101 (MQ=60)
                                                                                    TCAAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTT              >  SRR4302518.3636467/1‑101 (MQ=60)
                                                                                    NNNAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTT              <  SRR4302518.11770171/101‑1 (MQ=60)
                                                                                     NNNAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTC             <  SRR4302518.8729778/101‑1 (MQ=60)
                                                                                     NNNAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTCTAAATTTC             <  SRR4302518.13547185/101‑1 (MQ=60)
                                                                                      NNNAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCT            <  SRR4302518.8911415/101‑1 (MQ=60)
                                                                                      AAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCT            <  SRR4302518.6863/101‑1 (MQ=60)
                                                                                      AAAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTNNN            >  SRR4302518.10850034/1‑101 (MQ=60)
                                                                                       AAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTNNN           >  SRR4302518.14266568/1‑101 (MQ=60)
                                                                                       AAAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTT           >  SRR4302518.5024685/1‑101 (MQ=60)
                                                                                        AAGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTA          <  SRR4302518.229956/101‑1 (MQ=60)
                                                                                         NNNACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTAT         <  SRR4302518.10963728/101‑1 (MQ=60)
                                                                                         NNNACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTAT         <  SRR4302518.10244641/101‑1 (MQ=60)
                                                                                         AGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTAT         >  SRR4302518.841200/1‑101 (MQ=60)
                                                                                         AGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTAT         >  SRR4302518.4489264/1‑101 (MQ=60)
                                                                                         AGGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTNNN         >  SRR4302518.9695073/1‑101 (MQ=60)
                                                                                          NNNCGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTATG        <  SRR4302518.12424757/101‑1 (MQ=60)
                                                                                          GGACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTATG        <  SRR4302518.3158846/101‑1 (MQ=60)
                                                                                          GGACGCAATGCGCTCAGTAGCGCTTCACGCCATCGTTGATTTTTTCAACAGGCATAACTCGCCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTATG        <  SRR4302518.2808588/101‑1 (MQ=60)
                                                                                           GTCGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTATGT       <  SRR4302518.2772732/101‑1 (MQ=60)
                                                                                           GACGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTATGT       <  SRR4302518.4460629/101‑1 (MQ=60)
                                                                                             CGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTATGNNN     >  SRR4302518.13494086/1‑101 (MQ=60)
                                                                                             CGCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTATGNNN     >  SRR4302518.15103644/1‑101 (MQ=60)
                                                                                              GCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCTAGCGGAAACCGCTCTACGGAGGGGTAAATGTCTTATGTACC    >  SRR4302518.2914546/1‑101 (MQ=60)
                                                                                              GCAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTATGTACC    >  SRR4302518.1089612/1‑101 (MQ=60)
                                                                                               CAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTATGTACCA   <  SRR4302518.1365427/101‑1 (MQ=60)
                                                                                               CAATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTATGTACCA   >  SRR4302518.3310491/1‑101 (MQ=60)
                                                                                               CAATGCGCTCAGTCGCACTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCCCCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTATGTACCA   <  SRR4302518.3788212/101‑1 (MQ=60)
                                                                                                AATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGTATTAAATTTCTTATGTACCAT  >  SRR4302518.2598908/1‑101 (MQ=60)
                                                                                                AATGCGCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTATGTACNNN  >  SRR4302518.13101456/1‑101 (MQ=60)
                                                                                                    |                                                                                                
TTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAGGAGGACGGCCGTTGGTAATTAAAGGTGCTTACCATGAGATCCTTTTTGAAAAGGACGCAATGGCCTCAGTCGCGCTCCACGCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAAATTTCTTATGTACCAG  >  NC_000913/4007618‑4007814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑