Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 245 579.6 28867798 97.7% 28203838 96.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,008,393 C→G A159G (GCT→GGT)  b4555 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,008,3930CG100.0% 779.5 / NA 197A159G (GCT→GGT) b4555hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (85/112);  total (85/112)

Only 100 of 209 total aligned reads displayed.

GGGGCGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTGGAAGTGATGGCGGGCGGCGTTTC‑AAAGGCCATGCGCTGGAAG‑GCTGGCG‑‑‑‑AACT‑G‑GCTAC‑AG‑C‑TGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCG  >  NC_000913/4008306‑4008461
                                                                                         |                                                                                
ggggCGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCAAGTGCTGGAAGCGGTGGCG‑‑‑‑a                                                                        >  2:3025377/1‑96 (MQ=255)
  ggCGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCG‑‑‑‑aag                                                                      <  1:12447895/96‑2 (MQ=255)
   gCGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCG‑‑‑‑aaga                                                                     <  1:1268475/96‑3 (MQ=255)
   gCGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCG‑‑‑‑aaga                                                                     <  2:599041/96‑3 (MQ=255)
    cGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCG‑‑‑‑aagaa                                                                    <  2:1345660/96‑4 (MQ=255)
    cGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCG‑‑‑‑aagaa                                                                    >  1:3301232/1‑93 (MQ=255)
    cGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCG‑‑‑‑aagaa                                                                    <  1:3615729/96‑4 (MQ=255)
     gATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCG‑‑‑‑aagaaa                                                                   <  2:10998203/96‑5 (MQ=255)
                                     cTGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGtt                                    <  2:3761128/96‑1 (MQ=255)
                                      tGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGttt                                   <  1:5021437/96‑2 (MQ=255)
                                      tGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGttt                                   <  2:5615949/96‑2 (MQ=255)
                                      tGTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGttt                                   <  1:6839357/96‑2 (MQ=255)
                                       gTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGtttg                                  <  1:9864495/96‑3 (MQ=255)
                                       gTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGtttg                                  >  2:12489683/1‑94 (MQ=255)
                                       gTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGtttg                                  >  2:9040325/1‑94 (MQ=255)
                                       gTCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGtttg                                  <  2:5241731/96‑3 (MQ=255)
                                       gTCTGGAAGTGAGGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGtttg                                  <  1:6083090/96‑3 (MQ=255)
                                        tCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGtttgg                                 >  2:7833193/1‑93 (MQ=255)
                                         cTGGATGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGtttggt                                <  1:4762551/96‑5 (MQ=255)
                                         cTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGtttggt                                >  2:446608/1‑92 (MQ=255)
                                                 gATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGAt                        <  2:6878174/96‑1 (MQ=255)
                                                 gATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGAt                        <  1:1072092/96‑1 (MQ=255)
                                                 gATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGAGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGAt                        <  2:1017290/96‑1 (MQ=255)
                                                  aTGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATg                       <  2:161048/96‑1 (MQ=255)
                                                  aTGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATg                       <  1:11708699/96‑1 (MQ=255)
                                                   tGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGAtcg                      >  1:12886350/1‑94 (MQ=255)
                                                    ggcgggcgGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTCC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGaa                     >  2:9543973/1‑96 (MQ=255)
                                                    ggcgggcgGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGaa                     <  1:13237122/96‑1 (MQ=255)
                                                    ggcgggcgGCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGaa                     <  1:13138591/96‑1 (MQ=255)
                                                     gcgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAac                    <  2:8316868/96‑1 (MQ=255)
                                                     gcgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAac                    <  1:3148361/96‑1 (MQ=255)
                                                     gcgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAac                    <  1:5164257/96‑1 (MQ=255)
                                                     gcgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAac                    <  1:10467316/96‑1 (MQ=255)
                                                      cgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAacg                   <  1:7580615/96‑1 (MQ=255)
                                                      cgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAacg                   <  1:4331098/96‑1 (MQ=255)
                                                      cgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAacg                   <  2:2577626/96‑1 (MQ=255)
                                                      cgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGAGTTTGGTGACGGGATGAacg                   >  2:9604794/1‑96 (MQ=255)
                                                       gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAacga                  >  2:8797669/1‑96 (MQ=255)
                                                       gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAacga                  >  2:10446427/1‑96 (MQ=255)
                                                       gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAacga                  >  1:6278348/1‑96 (MQ=255)
                                                       gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAacga                  >  2:6681137/1‑96 (MQ=255)
                                                       gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAacga                  <  1:6963532/96‑1 (MQ=255)
                                                       gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAacga                  >  2:4189712/1‑96 (MQ=255)
                                                       gggcggCGTGTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAacga                  <  1:380389/96‑1 (MQ=255)
                                                        gtcggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAacgac                 <  1:12859185/94‑1 (MQ=255)
                                                        ggcggcGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTACAAC‑C‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAacgac                 <  2:7490016/96‑1 (MQ=255)
                                                        ggcggcGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAacgac                 >  2:11684656/1‑96 (MQ=255)
                                                        ggcggcGTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAacgac                 >  2:2688130/1‑96 (MQ=255)
                                                        ggcggcGTTTCAAAAGGCCATGCGCTGGAAGCGGGGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAacgac                 >  1:1566776/1‑96 (MQ=255)
                                                        ggcggcGTATCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACT‑GAGCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAacgtc                 <  1:7369695/96‑3 (MQ=255)
                                                         gcggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAacgacg                <  2:12997738/96‑1 (MQ=255)
                                                          cggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGc               >  1:8004230/1‑96 (MQ=255)
                                                          cggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGc               <  1:934936/96‑1 (MQ=255)
                                                          cggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGc               >  2:7099164/1‑96 (MQ=255)
                                                           ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGcc              >  1:7394350/1‑96 (MQ=255)
                                                           ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGcc              <  1:3236505/96‑1 (MQ=255)
                                                           ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GATAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGcc              <  1:11448052/96‑1 (MQ=255)
                                                           ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGACGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGAATTGCGTTTGGTGACGGGATGAACGACGcc              >  2:14090384/1‑96 (MQ=255)
                                                           ggcgTTTCAAAAGGCCATGCGCTGGAAGCGGGGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGcc              >  2:1886487/1‑96 (MQ=255)
                                                            gcgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCg             >  2:4457700/1‑96 (MQ=255)
                                                             cgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGa            >  1:4288595/1‑96 (MQ=255)
                                                             cgTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGa            >  1:4703266/1‑96 (MQ=255)
                                                              gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGaa           >  2:7698105/1‑96 (MQ=255)
                                                              gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGaa           <  2:3393939/96‑1 (MQ=255)
                                                              gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGaa           >  2:6175746/1‑96 (MQ=255)
                                                              gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGaa           <  1:10187737/96‑1 (MQ=255)
                                                              gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGaa           >  2:13238161/1‑96 (MQ=255)
                                                              gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGaa           >  1:11103465/1‑96 (MQ=255)
                                                              gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGaa           <  2:13116170/96‑1 (MQ=255)
                                                              gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGaa           >  1:1305043/1‑96 (MQ=255)
                                                              gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGaa           >  1:13948971/1‑96 (MQ=255)
                                                              gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGaa           >  1:9484328/1‑96 (MQ=255)
                                                              gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGaa           >  1:3477950/1‑96 (MQ=255)
                                                              gTTTCAAAAGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGaa           <  1:711031/96‑1 (MQ=255)
                                                                ttcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAt         <  2:1977746/92‑1 (MQ=255)
                                                                ttcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAt         <  2:10664278/92‑1 (MQ=255)
                                                                ttcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAt         <  1:1861003/92‑1 (MQ=255)
                                                                ttcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAt         <  2:5010472/92‑1 (MQ=255)
                                                                 tcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATg        <  2:5734103/93‑1 (MQ=255)
                                                                 tcaaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATg        >  2:10076470/4‑96 (MQ=255)
                                                                 tcaaaaGGCCATGCGCTGGAAGCAGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATg        <  1:10058740/93‑1 (MQ=255)
                                                                  caaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AG‑CATGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGc       >  1:2711779/3‑96 (MQ=255)
                                                                   aaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGca      <  1:4128963/95‑2 (MQ=255)
                                                                   aaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCt      >  1:12519757/2‑96 (MQ=255)
                                                                   aaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCt      <  2:2208879/95‑1 (MQ=255)
                                                                   aaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCt      <  1:13381048/95‑1 (MQ=255)
                                                                   aaaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGAAGCCGAAATGCt      >  1:2024656/2‑96 (MQ=255)
                                                                    aaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTg     >  1:7888015/1‑96 (MQ=255)
                                                                    aaaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTg     <  1:14256729/96‑1 (MQ=255)
                                                                     aaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGt    <  1:5889603/96‑1 (MQ=255)
                                                                     aaGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGt    <  1:4934091/96‑1 (MQ=255)
                                                                      aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGtt   <  1:8861793/96‑2 (MQ=255)
                                                                      aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTc   <  1:1745716/96‑1 (MQ=255)
                                                                      aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTc   >  2:8011587/1‑96 (MQ=255)
                                                                      aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTc   <  1:3005409/96‑1 (MQ=255)
                                                                      aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTc   >  1:4364937/1‑96 (MQ=255)
                                                                      aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTc   >  2:1856400/1‑96 (MQ=255)
                                                                      aGGCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTCACGGGATGAACGACGCCGAAATGCTGTc   <  2:13877228/96‑1 (MQ=255)
                                                                       ggCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTct  <  2:9449805/96‑2 (MQ=255)
                                                                       ggCCATGCGCTGGAAGCGGTGGCGAAGAAACTGG‑GCTAC‑AGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCg  <  2:8897623/96‑1 (MQ=255)
                                                                                         |                                                                                
GGGGCGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTGGAAGTGATGGCGGGCGGCGTTTC‑AAAGGCCATGCGCTGGAAG‑GCTGGCG‑‑‑‑AACT‑G‑GCTAC‑AG‑C‑TGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCG  >  NC_000913/4008306‑4008461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAACGCTCGTTGGGGCGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTGGAAGTGATGGCGGGCGGCG‑TTTC‑AAAGGCCATGCGCTGGAAG‑GC‑‑TGGCG‑‑‑‑AA‑‑‑‑CT‑GGCTACAG‑C‑TGAAGGATTG‑ATTGCG‑‑TT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGG  >  NC_000913/4008295‑4008468
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TAACGCTCGTTGGGGCGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTGGAAGTGATGGCGGGGGGCGCTTTC‑AAAGGCCATGCGCTGGAAG‑NN‑‑N                                                                                            >  SRR4302518.8191341/1‑101 (MQ=60)
 NNNGCTCGTTGGGGCGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTGGAAGTGATGGCGGGCGGCG‑TTTCAAAAGGCCATGCGCTGGAAGCGC‑‑T                                                                                            <  SRR4302518.11115366/101‑1 (MQ=60)
                                                       NNNGTGATGGCGGGCGGCG‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGAT                               <  SRR4302518.11741891/101‑1 (MQ=18)
                                                       GAAGTGATGGCGGGCGGCG‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGAT                               <  SRR4302518.1114364/101‑1 (MQ=23)
                                                        NNNTGATGGCGGGCGGCG‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATG                              <  SRR4302518.13664866/101‑1 (MQ=17)
                                                        NNNTGATGGCGGGCGGCG‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATG                              <  SRR4302518.12262059/101‑1 (MQ=17)
                                                          GTGATGGCGGGCGGCG‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAA                            <  SRR4302518.4579327/101‑1 (MQ=18)
                                                           TGATGGCGGGCGGCG‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAAC                           <  SRR4302518.1641741/101‑1 (MQ=18)
                                                           TGATGGCGGGCGGCG‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAAC                           <  SRR4302518.3295537/101‑1 (MQ=18)
                                                           TGATGGCGGGCGGCG‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAAC                           <  SRR4302518.5430644/101‑1 (MQ=18)
                                                           GGATGGCGGGCGGCG‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAAC                           <  SRR4302518.4720110/101‑1 (MQ=17)
                                                            GATGGCGGGCGGCG‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACG                          <  SRR4302518.2106652/101‑1 (MQ=18)
                                                            GATGGCGGGCGGCG‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACG                          <  SRR4302518.4547432/101‑1 (MQ=18)
                                                             ATGGCGGGCGGCG‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGA                         <  SRR4302518.7333498/101‑1 (MQ=18)
                                                               GGCGGGCGGCG‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACG                       <  SRR4302518.1880780/101‑1 (MQ=18)
                                                                GCGGGCGGCG‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGC                      <  SRR4302518.971059/101‑1 (MQ=18)
                                                                 CGGGCGGCG‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCC                     <  SRR4302518.3386956/101‑1 (MQ=18)
                                                                 CGGGCGGCG‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCC                     <  SRR4302518.7255353/101‑1 (MQ=18)
                                                                  GGGCGGCG‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCG                    >  SRR4302518.6598371/1‑101 (MQ=18)
                                                                   GGCGGCG‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGA                   >  SRR4302518.4514232/1‑101 (MQ=18)
                                                                    GCGGCG‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAA                  <  SRR4302518.738790/101‑1 (MQ=18)
                                                                      GGCG‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAAT                >  SRR4302518.2801977/1‑101 (MQ=18)
                                                                      GGCG‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAAT                <  SRR4302518.1944757/101‑1 (MQ=18)
                                                                        CG‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGC              >  SRR4302518.4500920/1‑101 (MQ=20)
                                                                        CG‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGC              >  SRR4302518.4950324/1‑101 (MQ=20)
                                                                         G‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATNNN             >  SRR4302518.10207111/1‑101 (MQ=17)
                                                                         G‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATNNN             >  SRR4302518.9944202/1‑101 (MQ=17)
                                                                         G‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATNNN             >  SRR4302518.7959043/1‑101 (MQ=17)
                                                                         G‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATNNN             >  SRR4302518.14587260/1‑101 (MQ=17)
                                                                         G‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATNNN             >  SRR4302518.11622897/1‑101 (MQ=17)
                                                                         G‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCT             >  SRR4302518.5282400/1‑101 (MQ=22)
                                                                         G‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCT             >  SRR4302518.3639771/1‑101 (MQ=22)
                                                                         G‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCT             <  SRR4302518.4019725/101‑1 (MQ=22)
                                                                         G‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCG             >  SRR4302518.1358678/1‑101 (MQ=20)
                                                                         G‑TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCA             <  SRR4302518.4329339/101‑1 (MQ=20)
                                                                          nnntcaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCT             <  SRR4302518.13996534/95‑1 (MQ=23)
                                                                          nnntcaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCT             <  SRR4302518.7710979/95‑1 (MQ=23)
                                                                           nnncaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTG            <  SRR4302518.14914176/96‑1 (MQ=24)
                                                                           gttcaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTG            <  SRR4302518.3964086/96‑1 (MQ=24)
                                                                           TTTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTG            >  SRR4302518.3015330/1‑101 (MQ=23)
                                                                            nnnaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGT           <  SRR4302518.12117278/97‑1 (MQ=26)
                                                                            TTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGT           <  SRR4302518.5241394/101‑1 (MQ=25)
                                                                            TTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGT           <  SRR4302518.5190948/101‑1 (MQ=25)
                                                                            TTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGT           >  SRR4302518.3348434/1‑101 (MQ=25)
                                                                            TTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGT           <  SRR4302518.5124865/101‑1 (MQ=25)
                                                                            TTCAAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGT           <  SRR4302518.6188071/101‑1 (MQ=25)
                                                                            NNN‑AAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCGTCTG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTC          <  SRR4302518.9885816/101‑1 (MQ=18)
                                                                            NNN‑AAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTT          <  SRR4302518.9291507/101‑1 (MQ=25)
                                                                             tcaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGTTTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTC          >  SRR4302518.3061156/4‑101 (MQ=19)
                                                                             tcaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTNNN          >  SRR4302518.14030986/4‑101 (MQ=22)
                                                                             tcaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTNNN          >  SRR4302518.9055649/4‑101 (MQ=22)
                                                                             tcaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTC          <  SRR4302518.1495681/98‑1 (MQ=27)
                                                                             tcaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTC          <  SRR4302518.1823641/98‑1 (MQ=27)
                                                                             tcaAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTC          <  SRR4302518.3146525/98‑1 (MQ=27)
                                                                              caAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCG         <  SRR4302518.295536/99‑1 (MQ=29)
                                                                              caAAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAG‑CATGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCG         >  SRR4302518.2840817/3‑101 (MQ=29)
                                                                              A‑AAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGgcgg        >  SRR4302518.3644569/1‑97 (MQ=24)
                                                                              A‑AAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTNNN        >  SRR4302518.13107698/1‑101 (MQ=25)
                                                                              A‑AAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGAAGCCGAAATGCTGTCGA        >  SRR4302518.2118040/1‑101 (MQ=22)
                                                                                AAAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCNNN       >  SRR4302518.8283886/1‑101 (MQ=27)
                                                                                 AAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG      <  SRR4302518.6669245/101‑1 (MQ=32)
                                                                                 AAGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATG      <  SRR4302518.897486/101‑1 (MQ=32)
                                                                                 AAGGCCATGCGCTGG‑AG‑GCGGTGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCGGAAATGCTGTCGATG      >  SRR4302518.4422904/1‑101 (MQ=23)
                                                                                  AGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG     >  SRR4302518.6644713/1‑101 (MQ=34)
                                                                                  AGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG     >  SRR4302518.5495458/1‑101 (MQ=34)
                                                                                  AGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG     <  SRR4302518.466545/101‑1 (MQ=34)
                                                                                  AGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG     >  SRR4302518.4583866/1‑101 (MQ=34)
                                                                                  AGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG     >  SRR4302518.3165368/1‑101 (MQ=34)
                                                                                  AGGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGG     >  SRR4302518.110381/1‑101 (MQ=34)
                                                                                   GGCCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC    <  SRR4302518.2965700/101‑1 (MQ=36)
                                                                                     CCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGNNN  >  SRR4302518.11275641/1‑101 (MQ=34)
                                                                                     CCATGCGCTGGAAGCGG‑‑TGGCGAAGAAA‑‑‑‑CTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGNNN  >  SRR4302518.10510098/1‑101 (MQ=34)
                                                                                     CCATGCGCTGGAAGCGG‑‑TGGCG‑‑‑‑AAGCATCTGGGCTACAGCC‑TGAAGGATTGTATTGCG‑‑TTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGG  <  SRR4302518.7312009/101‑1 (MQ=39)
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TAACGCTCGTTGGGGCGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTGGAAGTGATGGCGGGCGGCG‑TTTC‑AAAGGCCATGCGCTGGAAG‑GC‑‑TGGCG‑‑‑‑AA‑‑‑‑CT‑GGCTACAG‑C‑TGAAGGATTG‑ATTGCG‑‑TT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGG  >  NC_000913/4008295‑4008468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑